JP5894180B2 - 深さ分解能が向上した顕微鏡検査 - Google Patents

深さ分解能が向上した顕微鏡検査 Download PDF

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JP5894180B2
JP5894180B2 JP2013539209A JP2013539209A JP5894180B2 JP 5894180 B2 JP5894180 B2 JP 5894180B2 JP 2013539209 A JP2013539209 A JP 2013539209A JP 2013539209 A JP2013539209 A JP 2013539209A JP 5894180 B2 JP5894180 B2 JP 5894180B2
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JP2014501915A5 (enExample
JP2014501915A (ja
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カルクブレナー、トーマス
リッペルト、ヘルムート
クレッペ、インゴ
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Carl Zeiss Microscopy GmbH
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6447—Fluorescence; Phosphorescence by visual observation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Multimedia (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
JP2013539209A 2010-11-16 2011-11-11 深さ分解能が向上した顕微鏡検査 Active JP5894180B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102010044013A DE102010044013A1 (de) 2010-11-16 2010-11-16 Tiefenauflösungsgesteigerte Mikroskopie
DE102010044013.2 2010-11-16
PCT/EP2011/069918 WO2012065910A1 (de) 2010-11-16 2011-11-11 Tiefenauflösungsgesteigerte mikroskopie

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JP2014501915A JP2014501915A (ja) 2014-01-23
JP2014501915A5 JP2014501915A5 (enExample) 2014-12-25
JP5894180B2 true JP5894180B2 (ja) 2016-03-23

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US (1) US9201011B2 (enExample)
EP (1) EP2641078B1 (enExample)
JP (1) JP5894180B2 (enExample)
DE (1) DE102010044013A1 (enExample)
WO (1) WO2012065910A1 (enExample)

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DE102012201003B4 (de) * 2012-01-24 2024-07-25 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie
JP6338597B2 (ja) * 2013-01-09 2018-06-06 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ 無線周波数多重励起を用いた蛍光イメージングのための装置及び方法
DE102013102988A1 (de) * 2013-03-22 2014-09-25 Leica Microsystems Cms Gmbh Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten
DE102013208926A1 (de) * 2013-05-14 2014-11-20 Carl Zeiss Microscopy Gmbh Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie
DE102013208927A1 (de) * 2013-05-14 2014-11-20 Carl Zeiss Microscopy Gmbh Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie
DE102013009042A1 (de) 2013-05-28 2014-12-04 Carl Zeiss Microscopy Gmbh Lumineszenzmikroskopie
DE102013106895B4 (de) * 2013-07-01 2015-09-17 Leica Microsystems Cms Gmbh Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten
DE102013216124A1 (de) * 2013-08-14 2015-02-19 Carl Zeiss Microscopy Gmbh Hochauflösende 3D-Fluoreszenzmikroskopie
JP6223157B2 (ja) * 2013-12-04 2017-11-01 オリンパス株式会社 三次元画像撮像方法、三次元画像解析方法、及び三次元画像撮像システム
DE102013226277A1 (de) * 2013-12-17 2015-06-18 Leica Microsystems Cms Gmbh Verfahren und Vorrichtung zum Untersuchen einer Probe mittels optischer Projektionstomografie
WO2015108846A1 (en) 2014-01-14 2015-07-23 Applied Scientific Instrumentation, Inc. Light sheet generator
ES2959506T3 (es) 2014-03-18 2024-02-26 Univ California Citómetro de flujo paralelo que usa multiplexación por radiofrecuencia, y método
EP2963672A1 (en) * 2014-06-30 2016-01-06 FEI Company Computational scanning microscopy with improved resolution
JP6510239B2 (ja) * 2015-01-08 2019-05-08 オリンパス株式会社 蛍光顕微鏡
DE102015209756A1 (de) * 2015-05-28 2016-12-01 Carl Zeiss Microscopy Gmbh Anordnung und Verfahren zur Lichtblattmikroskopie
JP6856635B2 (ja) 2015-10-13 2021-04-07 オメガ バイオシステムズ インコーポレイテッド マルチモードの蛍光撮像フローサイトメトリシステム
AU2017234815B2 (en) 2016-03-17 2022-11-03 Becton, Dickinson And Company Cell sorting using a high throughput fluorescence flow cytometer
US10876970B2 (en) * 2016-04-12 2020-12-29 The Board Of Regents Of The University Of Texas System Light-sheet microscope with parallelized 3D image acquisition
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FR3055704B1 (fr) * 2016-09-02 2018-10-05 Centre National De La Recherche Scientifique Procede de detection d'especes fluorescentes reversiblement photocommutables a haute frequence
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US10690898B2 (en) * 2016-09-15 2020-06-23 Molecular Devices (Austria) GmbH Light-field microscope with selective-plane illumination
JP6932036B2 (ja) * 2017-07-31 2021-09-08 シスメックス株式会社 細胞撮像方法、細胞撮像装置、粒子撮像方法および粒子撮像装置
JP2019086529A (ja) * 2017-11-01 2019-06-06 株式会社ニコン 顕微鏡、照明装置、及び観察方法
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DE102010044013A1 (de) 2012-05-16
US20130302905A1 (en) 2013-11-14
US9201011B2 (en) 2015-12-01
EP2641078B1 (de) 2015-01-07
JP2014501915A (ja) 2014-01-23
WO2012065910A1 (de) 2012-05-24
EP2641078A1 (de) 2013-09-25

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