DD284053A5 - Ein verfahren zur verstaerkung von nucleotiden sequenzen - Google Patents

Ein verfahren zur verstaerkung von nucleotiden sequenzen Download PDF

Info

Publication number
DD284053A5
DD284053A5 DD89331212A DD33121289A DD284053A5 DD 284053 A5 DD284053 A5 DD 284053A5 DD 89331212 A DD89331212 A DD 89331212A DD 33121289 A DD33121289 A DD 33121289A DD 284053 A5 DD284053 A5 DD 284053A5
Authority
DD
German Democratic Republic
Prior art keywords
nucleic acid
vectorette
primer
initiation
target nucleic
Prior art date
Application number
DD89331212A
Other languages
German (de)
English (en)
Inventor
Alexander F Markham
John C Smith
Rashida Anwar
Original Assignee
��������@��������@����������@���k��
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ��������@��������@����������@���k�� filed Critical ��������@��������@����������@���k��
Publication of DD284053A5 publication Critical patent/DD284053A5/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08GMACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G77/00Macromolecular compounds obtained by reactions forming a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon in the main chain of the macromolecule
    • C08G77/42Block-or graft-polymers containing polysiloxane sequences
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DD89331212A 1988-07-28 1989-07-27 Ein verfahren zur verstaerkung von nucleotiden sequenzen DD284053A5 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB888818020A GB8818020D0 (en) 1988-07-28 1988-07-28 Method for amplification of nucleotide sequences

Publications (1)

Publication Number Publication Date
DD284053A5 true DD284053A5 (de) 1990-10-31

Family

ID=10641273

Family Applications (1)

Application Number Title Priority Date Filing Date
DD89331212A DD284053A5 (de) 1988-07-28 1989-07-27 Ein verfahren zur verstaerkung von nucleotiden sequenzen

Country Status (24)

Country Link
EP (1) EP0356021A3 (pt)
JP (1) JPH02174679A (pt)
KR (1) KR910003100A (pt)
CN (1) CN1040220A (pt)
AU (1) AU635212B2 (pt)
BG (1) BG89380A (pt)
BR (1) BR8903792A (pt)
DD (1) DD284053A5 (pt)
DK (1) DK367389A (pt)
FI (1) FI893589A (pt)
GB (3) GB8818020D0 (pt)
HU (1) HUT53944A (pt)
IE (1) IE62579B1 (pt)
IL (1) IL91049A0 (pt)
IS (1) IS3495A7 (pt)
JO (1) JO1598B1 (pt)
MT (1) MTP1043B (pt)
NO (1) NO893054L (pt)
NZ (1) NZ230053A (pt)
PT (1) PT91292B (pt)
TN (1) TNSN89084A1 (pt)
YU (1) YU151789A (pt)
ZA (1) ZA895473B (pt)
ZW (1) ZW8789A1 (pt)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes
GB8920211D0 (en) * 1989-09-07 1989-10-18 Ici Plc Diagnostic method
IE65771B1 (en) * 1990-01-25 1995-11-15 Zeneca Ltd Amplification of nucleotide sequences using vectorette units
CA2042808C (en) * 1990-05-17 2000-04-25 Toshihiko Kishimoto Method of extracting particular nucleic acid fragment
GB9011454D0 (en) * 1990-05-22 1990-07-11 Medical Res Council Polynucleotide amplification
AU1426392A (en) * 1991-01-22 1992-08-27 Board Of Regents, The University Of Texas System 5' and 3' polymerase chain reaction walking from known dna sequences
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5597694A (en) * 1993-10-07 1997-01-28 Massachusetts Institute Of Technology Interspersed repetitive element-bubble amplification of nucleic acids
US5516653A (en) 1993-12-28 1996-05-14 Synaptic Pharmaceutical Corporation DNA encoding a human neuropeptide Y/peptide YY/pancreatic polypeptide receptor (Y4) and uses thereof
RU2111254C1 (ru) * 1994-07-11 1998-05-20 Институт молекулярной биологии им.В.А.Энгельгардта РАН СПОСОБ ДЕТЕКЦИИ ДИФФЕРЕНЦИАЛЬНО ЭКСПРЕССИРУЮЩИХСЯ МАТРИЧНЫХ РНК И КЛОНИРОВАНИЯ СООТВЕТСТВУЮЩИХ ИМ ФРАГМЕНТОВ кДНК
EP0804618B1 (en) * 1994-11-28 1999-01-27 E.I. Du Pont De Nemours And Company Compound microsatellite primers for the detection of genetic polymorphisms
EP0873415B1 (en) * 1995-04-14 2008-09-10 University Of Alabama Research Foundation Fusion protein delivery system and uses thereof
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
WO1997019193A2 (en) 1995-11-21 1997-05-29 Yale University Unimolecular segment amplification and detection
EP1007730A4 (en) * 1996-08-30 2004-04-28 Invitrogen Corp METHODS OF IDENTIFYING AND ISOLATING SPECIFIC NUCLEOTIDE SEQUENCES IN cDNA AND GENOMIC DNA
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
US7622300B2 (en) 1998-06-03 2009-11-24 Kappes John C Trans-lentiviral vector particles and transduction of eukaryotic cells therewith
US6555342B1 (en) 1998-06-03 2003-04-29 Uab Research Foundation Fusion protein delivery system and uses thereof
US6316229B1 (en) 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
US6255082B1 (en) 1998-09-15 2001-07-03 Yale University Artificial long terminal repeat vectors
AU770993B2 (en) 1998-09-15 2004-03-11 Yale University Molecular cloning using rolling circle amplification
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
DE19849348A1 (de) * 1998-10-26 2000-04-27 Univ Ludwigs Albert Linear Amplification mediated PCR (=LAM PCR)
AU2001271722B2 (en) 2000-06-30 2006-04-13 Qiagen, Gmbh Signal amplification with lollipop probes
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6573051B2 (en) 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
KR100758610B1 (ko) * 2001-05-07 2007-09-13 (주)바이오니아 염기서열이 전혀 알려지지 아니한 디옥시리보핵산의선택적 증폭 방법
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
WO2007044671A2 (en) 2005-10-06 2007-04-19 Lucigen Corporation Thermostable viral polymerases and methods of use
SG169356A1 (en) 2006-02-08 2011-03-30 Illumina Cambridge Ltd Method for sequencing a polynucleotide template
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2011025477A1 (en) 2009-08-25 2011-03-03 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
GB2501238B (en) 2012-03-16 2018-10-24 Cambridge Entpr Ltd Methods for obtaining liquid from a solid phase
ES2905448T3 (es) 2012-05-10 2022-04-08 Massachusetts Gen Hospital Métodos para determinar una secuencia nucleotídica
EP4219744A3 (en) 2014-01-27 2023-08-30 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
GB2566986A (en) * 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
AU5404890A (en) * 1989-02-14 1990-09-05 Biogen, Inc. Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them

Also Published As

Publication number Publication date
GB8917143D0 (en) 1989-09-13
GB8916572D0 (en) 1989-09-06
PT91292A (pt) 1990-02-08
FI893589A0 (fi) 1989-07-27
NO893054D0 (no) 1989-07-26
NO893054L (no) 1990-01-29
AU3896689A (en) 1990-03-22
IE62579B1 (en) 1995-04-05
YU151789A (en) 1991-04-30
IE892307L (en) 1990-01-28
KR910003100A (ko) 1991-02-26
EP0356021A3 (en) 1991-10-09
EP0356021A2 (en) 1990-02-28
HUT53944A (en) 1990-12-28
AU635212B2 (en) 1993-03-18
GB8818020D0 (en) 1988-09-01
BR8903792A (pt) 1990-03-20
PT91292B (pt) 1995-03-01
JPH02174679A (ja) 1990-07-06
JO1598B1 (en) 1989-12-16
DK367389D0 (da) 1989-07-25
ZW8789A1 (en) 1990-10-24
GB2221909B (en) 1992-11-11
IL91049A0 (en) 1990-02-09
IS3495A7 (is) 1990-01-29
MTP1043B (en) 1990-06-14
FI893589A (fi) 1990-01-29
BG89380A (bg) 1993-12-24
TNSN89084A1 (fr) 1991-02-04
CN1040220A (zh) 1990-03-07
DK367389A (da) 1990-01-29
NZ230053A (en) 1992-07-28
GB2221909A (en) 1990-02-21
ZA895473B (en) 1990-03-28

Similar Documents

Publication Publication Date Title
DD284053A5 (de) Ein verfahren zur verstaerkung von nucleotiden sequenzen
DE69432586T2 (de) Methode zum generieren von einzelsträngigen dna molekülen
DE3650762T2 (de) Kit zur Verwendung bei der Amplifizierung und beim Nachweis von Nukleinsäuresequenzen
DE69507646T2 (de) Mikrosatelliteverbindung für detektion genetisches polymorphismen
DE69432919T2 (de) Verfahren für den Nachweis spezifischer Polynukleotiden
DE68915202T2 (de) Verfahren zur Vermehrung und zum Nachweis von Nukleinsäuresequenzen.
DE3687287T3 (de) Verfahren zur Amplifikation von Nukleinsäuresequenzen.
DE69119083T2 (de) Direkte klonierung von pcr amplifizierten nukleinsäuren
DE69030955T2 (de) Nukleinsäureverstärkung
DE60034878T2 (de) Verfahren zur Amplifizierung einer Nukleinsäuresequenz unter Verwendung eines chimären Primers
DE69133582T2 (de) Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase
DE60025840T2 (de) Methode zum nachweis von variationen oder polymorphismen
DE69524653T2 (de) Amplifizierung von langen Nukleinsäuresequenzen mit PCR
DE69527171T2 (de) Strangverdrängungsamplifikation unter Verwendung von thermophilen Enzymen
DE69627189T2 (de) Verfahren zum nachweis und zur entfernung von mutantsequenzen, die während enzymatischer amplifikation entstehen
DE69603723T2 (de) Verfahren zum nachweis von nukleinsäuren
DE69804143T2 (de) Verfahren zur charakterisierung von nukleinsäuremolekulen, das die erzeugung von verlängerbaren aufwärtsgelegenen dns-fragmenten, die durch spaltung der nukleinsäure an einer abasischen stelle entsteht, umfasst
DE60029323T2 (de) Verfahren zur analyse der dna-methylierung mit hoher durchsatzrate
DE69713866T2 (de) Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung
DE69421277T2 (de) NUKLEINSäURE-SEQUENZANALYSE DURCH DIE METHODE DER PARALLELEN PRIMEREXTENSION
DE68925717T2 (de) Verfahren und Reagenzkombination zur Bestimmung von Nukleotidsequenzen
DE69636895T2 (de) Die Verwendung der 3'-wesentlichen Bearbeitungswirksamkeit von DNS-Polymerase
DE69605803T2 (de) Nachweis von fehlpaarungen durch spaltung mit resolvase auf einem festträger
DE60014350T2 (de) Verfahren zur erkennung und/oder analyse, unter verwendung von primertechniken, von einfachen nukleotidpolymorphismen in restriktionsfragmenten speziell aus amplifizierten restriktionsfragmenten generiert durch aflp
CA2073184A1 (en) Compositions and methods for analyzing genomic variation

Legal Events

Date Code Title Description
RPI Change in the person, name or address of the patentee (searches according to art. 11 and 12 extension act)
RPV Change in the person, the name or the address of the representative (searches according to art. 11 and 12 extension act)
ENJ Ceased due to non-payment of renewal fee