CY1123348T1 - Μεθοδος για την παρασκευη ενισχυμενων πολυδυναμων βλαστοκυτταρων απο μεσεγχυματικα βλαστοκυτταρα με τη χρηση κλασματος φλοροταννινης - Google Patents

Μεθοδος για την παρασκευη ενισχυμενων πολυδυναμων βλαστοκυτταρων απο μεσεγχυματικα βλαστοκυτταρα με τη χρηση κλασματος φλοροταννινης

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Publication number
CY1123348T1
CY1123348T1 CY20201100887T CY201100887T CY1123348T1 CY 1123348 T1 CY1123348 T1 CY 1123348T1 CY 20201100887 T CY20201100887 T CY 20201100887T CY 201100887 T CY201100887 T CY 201100887T CY 1123348 T1 CY1123348 T1 CY 1123348T1
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Cyprus
Prior art keywords
stem cells
enhanced
pluripotent stem
preparation
fraction
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CY20201100887T
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English (en)
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Sang Yeon Lee
Won Ju Jung
Ho Bin Kim
Min Sun Oh
Kye Ho Lee
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Bbhc
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Publication date
Priority claimed from PCT/KR2015/005183 external-priority patent/WO2015178728A1/ko
Application filed by Bbhc filed Critical Bbhc
Publication of CY1123348T1 publication Critical patent/CY1123348T1/el

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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/357—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having two or more oxygen atoms in the same ring, e.g. crown ethers, guanadrel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/76—Undefined extracts from plants
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C12N2506/025—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells from extra-embryonic cells, e.g. trophoblast, placenta
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1392—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from mesenchymal stem cells from other natural sources

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  • Health & Medical Sciences (AREA)
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  • Veterinary Medicine (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Η παρούσα εφεύρεση αναφέρεται σε μια σύνθεση μέσου για την αποδιαφοροποίηση των ενισχυμένων παντοδύναμων βλαστοκυττάρων, η οποία περιέχει ένα κλάσμα φλοροταννίνης, το οποίο εκχυλίστηκε και απομονώθηκε από έναν τύπο καφέ φυκιών που επιλέχθηκε από την ομάδα που αποτελείται από τα Ecklonia cava, Dictyopteris prolifera, Dictyota coriacea, Sargassum homeri, Ishige okamurai και τα παρόμοια. Επιπλέον, η παρούσα εφεύρεση αναφέρεται σε μια μέθοδο για την παρασκευή ενισχυμένων παντοδύναμων βλαστοκυττάρων χρησιμοποιώντας τη σύνθεση μέσου. Τα ενισχυμένα παντοδύναμα βλαστοκύτταρα μπορούν να παρασκευαστούν με ασφάλεια, εύκολα και αποτελεσματικά με τη χρήση μεσεγχυματικών βλαστοκυττάρων με τη χρήση της σύνθεσης μέσου της παρούσας εφεύρεσης, και τα παρασκευασμένα ενισχυμένα παντοδύναμα βλαστοκύτταρα μπορούν να διαφοροποιηθούν σε διάφορα κύτταρα και έτσι μπορούν να είναι χρήσιμα ως ένας κυτταρικός θεραπευτικός παράγοντας.
CY20201100887T 2014-05-23 2020-09-21 Μεθοδος για την παρασκευη ενισχυμενων πολυδυναμων βλαστοκυτταρων απο μεσεγχυματικα βλαστοκυτταρα με τη χρηση κλασματος φλοροταννινης CY1123348T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20140062526 2014-05-23
KR1020150071240A KR101699761B1 (ko) 2014-05-23 2015-05-21 플로로탄닌 분획물을 이용한 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법
PCT/KR2015/005183 WO2015178728A1 (ko) 2014-05-23 2015-05-22 플로로탄닌 분획물을 이용한 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법

Publications (1)

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CY1123348T1 true CY1123348T1 (el) 2021-12-31

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EP (1) EP3147352B1 (el)
JP (1) JP6839982B2 (el)
KR (1) KR101699761B1 (el)
CN (1) CN105960452B (el)
AU (1) AU2015262123B2 (el)
CA (1) CA2949949C (el)
CY (1) CY1123348T1 (el)
DK (1) DK3147352T3 (el)
ES (1) ES2820577T3 (el)
HR (1) HRP20201525T1 (el)
HU (1) HUE050877T2 (el)
IL (1) IL249170A0 (el)
LT (1) LT3147352T (el)
MX (1) MX2016015357A (el)
MY (1) MY179152A (el)
PH (1) PH12016502330A1 (el)
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PT (1) PT3147352T (el)
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Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6700175B2 (ja) 2013-11-01 2020-05-27 ビービーエイチシー・カンパニー・リミテッドＢｂｈｃ Ｃｏ．， Ｌｔｄ． 間葉系幹細胞から誘導多能性幹細胞を製造する方法及びその方法によって製造された誘導多能性幹細胞
KR101966315B1 (ko) * 2017-04-26 2019-04-08 제주대학교 산학협력단 감태 추출물과 괭생이모자반 추출물을 이용한 면역억제용 조성물
CN113194743A (zh) * 2018-12-05 2021-07-30 博塔医疗株式会社 包含褐藻多酚作为有效成分的用于改善食物香味的组合物
WO2021145742A1 (ko) * 2020-01-17 2021-07-22 주식회사 만나스 플로로탄닌을 유효성분으로 포함하는 운동 기능 개선용 조성물
CN111557869A (zh) * 2020-06-19 2020-08-21 湖北楚天香妆生物科技有限公司 昆布提取物的应用
CN114438037B (zh) * 2022-01-25 2024-06-04 深圳市乐土生物医药有限公司 一种制备诱导性间充质干细胞的方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5818874A (ja) * 1981-07-28 1983-02-03 Shin Kobe Electric Mach Co Ltd 鉛蓄電池の製造法
JP2932660B2 (ja) 1990-10-09 1999-08-09 松下電器産業株式会社 スクリーン印刷用スキージ装置
JP4146146B2 (ja) 2002-03-25 2008-09-03 熊本県 フロロタンニン類を主成分とする抗菌剤
US7910755B2 (en) * 2004-09-29 2011-03-22 Harbor Biosciences, Inc. Stem cell expansion and uses
BRPI0716762A2 (pt) * 2006-09-13 2013-09-24 Abbott Lab melhorias da cultura celular
WO2009157610A1 (en) * 2008-06-26 2009-12-30 Pusan National University Industry-University Cooperation Foundation Selenium dedifferentiated cell, preparation method and usage thereof
JP2010030917A (ja) * 2008-07-25 2010-02-12 Guraiko Materials:Kk 肝炎予防剤又は肝炎治療剤
KR101166257B1 (ko) * 2009-06-16 2012-07-19 한국생명공학연구원 미분화 다능성줄기세포의 제작, 유지 및 증식을 위한 뉴로펩타이드 ｙ를 포함하는 배지 조성물 및 이를 이용한 다능성줄기세포의 배양 방법
CN103210082A (zh) * 2010-09-15 2013-07-17 雷蒙特亚特特拉维夫大学有限公司 扩增胰岛β细胞和使其再分化的方法
KR101807704B1 (ko) * 2011-04-12 2017-12-13 차의과학대학교 산학협력단 역분화-증진제를 이용한 역분화 만능 줄기세포의 제조방법
US20150072416A1 (en) * 2012-05-29 2015-03-12 Korea Research Institute Of Bioscience And Biotech Metabolite for improving production, maintenance and proliferation of pluripotent stem cells, composition comprising the same, and method of culturing pluripotent stem cell using the same
KR101542849B1 (ko) * 2013-11-01 2015-08-10 주식회사 비비에이치씨 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 간세포로 분화시키는 방법
KR101544195B1 (ko) * 2013-11-01 2015-08-12 주식회사 비비에이치씨 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법 및 그 방법에 의해 제조된 유도만능 줄기세포
KR101542850B1 (ko) * 2013-11-01 2015-08-10 주식회사 비비에이치씨 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 지방세포로 분화시키는 방법
KR101542847B1 (ko) * 2013-11-01 2015-08-10 주식회사 비비에이치씨 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 신경세포로 분화시키는 방법
KR101542846B1 (ko) * 2013-11-01 2015-08-10 주식회사 비비에이치씨 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 연골세포로 분화시키는 방법
KR101542848B1 (ko) * 2013-11-01 2015-08-10 주식회사 비비에이치씨 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 골아세포로 분화시키는 방법

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LT3147352T (lt) 2020-12-10
PT3147352T (pt) 2020-09-29
CA2949949A1 (en) 2015-11-26
RU2668803C2 (ru) 2018-10-02
ES2820577T3 (es) 2021-04-21
US10131881B2 (en) 2018-11-20
HUE050877T2 (hu) 2021-01-28
EP3147352A4 (en) 2018-01-24
KR101699761B1 (ko) 2017-01-25
DK3147352T3 (da) 2020-09-28
US20170107481A1 (en) 2017-04-20
EP3147352A1 (en) 2017-03-29
AU2015262123B2 (en) 2018-04-19
JP2017516455A (ja) 2017-06-22
JP6839982B2 (ja) 2021-03-10
MX2016015357A (es) 2017-06-20
RS60817B1 (sr) 2020-10-30
AU2015262123A1 (en) 2016-08-25
PH12016502330B1 (en) 2017-02-06
CN105960452A (zh) 2016-09-21
SI3147352T1 (sl) 2021-01-29
PH12016502330A1 (en) 2017-02-06
IL249170A0 (en) 2017-01-31
CN105960452B (zh) 2019-11-22
RU2016150687A3 (el) 2018-06-25
SG11201609804RA (en) 2016-12-29
CA2949949C (en) 2023-03-28
EP3147352B1 (en) 2020-08-12
PL3147352T3 (pl) 2021-01-25
RU2016150687A (ru) 2018-06-25
MY179152A (en) 2020-10-29
KR20150135136A (ko) 2015-12-02
HRP20201525T1 (hr) 2021-02-19

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