CU24009B1 - Método de producción de lipasa recombinante lip2 - Google Patents
Método de producción de lipasa recombinante lip2Info
- Publication number
- CU24009B1 CU24009B1 CU2008000242A CU20080242A CU24009B1 CU 24009 B1 CU24009 B1 CU 24009B1 CU 2008000242 A CU2008000242 A CU 2008000242A CU 20080242 A CU20080242 A CU 20080242A CU 24009 B1 CU24009 B1 CU 24009B1
- Authority
- CU
- Cuba
- Prior art keywords
- lip2
- lipase
- recombinant
- carbon
- resistant
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Botany (AREA)
- Obesity (AREA)
- Diabetes (AREA)
- Biophysics (AREA)
- Nutrition Science (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se divulga un método para producir lipasa recombinante LIP2 ácido-resistente. El método utiliza en la etapa de precultivo células de una cepa de Yarrowia lipolytica productora en exceso de lipasa ácido-resistente, la cepa recombinante de Yarrowia lipolytica, YL-LIP2-6C depositada bajo el número I-3542 en la Colección Nacional de Cultivos de Microorganismos (C.N.C.M). Como medio de cultivo se utiliza Glucosa, KH2PO4, Na2HPO4, H3BO3, (NH4)2SO4, C5H8O4NNa, MgSO4, CaCl2, MnSO4 y ZnSO4; estando el medio enriquecido con oligoelementos y vitaminas. El método comprende una etapa de fermentación que incluye una fase de crecimiento celular con aporte de glucosa como única fuente de carbono y una etapa de inducción de la producción de lipasa con aporte de ácido oleico como única fuente de carbono.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/FR2006/001352 WO2007144475A1 (fr) | 2006-06-15 | 2006-06-15 | Procede de production de lipase, cellule de yarrowia lipolytica transformee apte a produire ladite lipase et leurs applications |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CU20080242A7 CU20080242A7 (es) | 2012-06-21 |
| CU24009B1 true CU24009B1 (es) | 2014-06-27 |
Family
ID=37591884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CU2008000242A CU24009B1 (es) | 2006-06-15 | 2008-12-15 | Método de producción de lipasa recombinante lip2 |
Country Status (23)
| Country | Link |
|---|---|
| US (2) | US8334130B2 (es) |
| EP (1) | EP2035556B1 (es) |
| JP (1) | JP5592648B2 (es) |
| KR (1) | KR101497159B1 (es) |
| CN (1) | CN101506359B (es) |
| AU (1) | AU2006344466B2 (es) |
| BR (1) | BRPI0621829B8 (es) |
| CA (1) | CA2657949C (es) |
| CR (1) | CR10515A (es) |
| CU (1) | CU24009B1 (es) |
| DK (1) | DK2035556T3 (es) |
| EA (1) | EA017963B1 (es) |
| EC (1) | ECSP099057A (es) |
| ES (1) | ES2550537T3 (es) |
| HK (1) | HK1129702A1 (es) |
| MX (1) | MX2008015978A (es) |
| NO (1) | NO342291B1 (es) |
| NZ (1) | NZ573601A (es) |
| PL (1) | PL2035556T3 (es) |
| PT (1) | PT2035556E (es) |
| SI (1) | SI2035556T1 (es) |
| UA (1) | UA97246C2 (es) |
| WO (1) | WO2007144475A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2327776A1 (en) | 2009-11-30 | 2011-06-01 | Institut National De La Recherche Agronomique | Method for the production of Very Long Chain Fatty Acids (VLCFA) by fermentation with a recombinant Yarrowia sp |
| EP2576795A1 (en) * | 2010-05-28 | 2013-04-10 | Csir | A method for producing a polypeptide in yarrowia lipolytica |
| CN102533692A (zh) * | 2010-12-08 | 2012-07-04 | 刘刚 | 一种酸性条件下具有降解脂肪能力的脂肪酶 |
| CN106754446B (zh) * | 2016-10-31 | 2020-05-19 | 华中科技大学 | 一种转化解脂耶氏酵母及其构建方法与应用 |
| JP2022545718A (ja) | 2019-08-30 | 2022-10-28 | コデクシス， インコーポレイテッド | 操作されたリパーゼ改変体 |
| FR3111559A1 (fr) * | 2020-06-18 | 2021-12-24 | Azurrx Biopharma, Inc. | Formulations non porcines et leurs procédés |
| KR102510688B1 (ko) | 2022-06-15 | 2023-03-17 | 아스티스(주) | 야로위아 리폴리티카 변이균주 및 이를 포함하는 기능성 화장료 조성물 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7116394A (en) * | 1993-07-01 | 1995-01-24 | Merck & Co., Inc. | Culture medium for recombinant yeasts |
| AU2000244105A1 (en) * | 2000-04-28 | 2001-11-12 | Laboratoires Mayoly Spindler | Cloning and expressing an acid-resistant extracellular lipase of yarrowia lipolytica |
| EP2298872A3 (en) * | 2004-09-30 | 2011-08-10 | Novozymes A/S | Polypeptides having lipase activity and polynucleotides encoding same |
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2006
- 2006-06-15 JP JP2009514831A patent/JP5592648B2/ja active Active
- 2006-06-15 ES ES06764790.9T patent/ES2550537T3/es active Active
- 2006-06-15 PL PL06764790T patent/PL2035556T3/pl unknown
- 2006-06-15 PT PT67647909T patent/PT2035556E/pt unknown
- 2006-06-15 BR BRPI0621829A patent/BRPI0621829B8/pt active IP Right Grant
- 2006-06-15 EA EA200970012A patent/EA017963B1/ru not_active IP Right Cessation
- 2006-06-15 CA CA2657949A patent/CA2657949C/fr active Active
- 2006-06-15 KR KR1020097000800A patent/KR101497159B1/ko active IP Right Grant
- 2006-06-15 MX MX2008015978A patent/MX2008015978A/es active IP Right Grant
- 2006-06-15 WO PCT/FR2006/001352 patent/WO2007144475A1/fr active Application Filing
- 2006-06-15 UA UAA200814355A patent/UA97246C2/ru unknown
- 2006-06-15 EP EP06764790.9A patent/EP2035556B1/fr active Active
- 2006-06-15 NZ NZ573601A patent/NZ573601A/en unknown
- 2006-06-15 DK DK06764790.9T patent/DK2035556T3/en active
- 2006-06-15 SI SI200631987T patent/SI2035556T1/sl unknown
- 2006-06-15 US US12/304,822 patent/US8334130B2/en active Active
- 2006-06-15 CN CN2006800549402A patent/CN101506359B/zh active Active
- 2006-06-15 AU AU2006344466A patent/AU2006344466B2/en active Active
-
2008
- 2008-12-09 NO NO20085119A patent/NO342291B1/no unknown
- 2008-12-15 CU CU2008000242A patent/CU24009B1/es active IP Right Grant
- 2008-12-17 CR CR10515A patent/CR10515A/es unknown
-
2009
- 2009-01-12 EC EC2009009057A patent/ECSP099057A/es unknown
- 2009-09-17 HK HK09108505.2A patent/HK1129702A1/zh unknown
-
2012
- 2012-08-10 US US13/571,869 patent/US8834867B2/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant of patent |