EA200970012A1 - Способ получения липазы, трансформированная клетка yarrowia lipolytica, способная продуцировать указанную липазу, и их применение - Google Patents

Способ получения липазы, трансформированная клетка yarrowia lipolytica, способная продуцировать указанную липазу, и их применение

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Publication number
EA200970012A1
EA200970012A1 EA200970012A EA200970012A EA200970012A1 EA 200970012 A1 EA200970012 A1 EA 200970012A1 EA 200970012 A EA200970012 A EA 200970012A EA 200970012 A EA200970012 A EA 200970012A EA 200970012 A1 EA200970012 A1 EA 200970012A1
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EA
Eurasian Patent Office
Prior art keywords
lipase
yarrowia lipolytica
abilant
application
obtaining
Prior art date
Application number
EA200970012A
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English (en)
Other versions
EA017963B1 (ru
Inventor
Ив Леблон
Николя Му
Ален Марти
Жан-Луи Юрибеларреа
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Лаборатуар Мейоли Спэндлер
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Publication date
Application filed by Лаборатуар Мейоли Спэндлер filed Critical Лаборатуар Мейоли Спэндлер
Publication of EA200970012A1 publication Critical patent/EA200970012A1/ru
Publication of EA017963B1 publication Critical patent/EA017963B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Epidemiology (AREA)
  • Obesity (AREA)
  • Immunology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Способ получения кислотоустойчивой рекомбинантной липазы Yarrowia lipolytica, в котором используется культуральная среда без каких-либо продуктов животного происхождения или неохарактеризованных смесей, таких как триптон, пептон или молочная сыворотка, а также его применение. Рекомбинантный штамм Yarrowia lipolytica, продуцирующий повышенное количество липазы Lip2, обозначенный YL-LIP2-6C и депонированный в Национальной Коллекции Культур Микроорганизмов (C.N.C.M.), под номером 1-3542, 15 декабря 2005, а также его применение.
EA200970012A 2006-06-15 2006-06-15 Способ получения липазы, трансформированная клетка yarrowia lipolytica, способная продуцировать указанную липазу, и их применение EA017963B1 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/FR2006/001352 WO2007144475A1 (fr) 2006-06-15 2006-06-15 Procede de production de lipase, cellule de yarrowia lipolytica transformee apte a produire ladite lipase et leurs applications

Publications (2)

Publication Number Publication Date
EA200970012A1 true EA200970012A1 (ru) 2009-10-30
EA017963B1 EA017963B1 (ru) 2013-04-30

Family

ID=37591884

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200970012A EA017963B1 (ru) 2006-06-15 2006-06-15 Способ получения липазы, трансформированная клетка yarrowia lipolytica, способная продуцировать указанную липазу, и их применение

Country Status (23)

Country Link
US (2) US8334130B2 (ru)
EP (1) EP2035556B1 (ru)
JP (1) JP5592648B2 (ru)
KR (1) KR101497159B1 (ru)
CN (1) CN101506359B (ru)
AU (1) AU2006344466B2 (ru)
BR (1) BRPI0621829B8 (ru)
CA (1) CA2657949C (ru)
CR (1) CR10515A (ru)
CU (1) CU24009B1 (ru)
DK (1) DK2035556T3 (ru)
EA (1) EA017963B1 (ru)
EC (1) ECSP099057A (ru)
ES (1) ES2550537T3 (ru)
HK (1) HK1129702A1 (ru)
MX (1) MX2008015978A (ru)
NO (1) NO342291B1 (ru)
NZ (1) NZ573601A (ru)
PL (1) PL2035556T3 (ru)
PT (1) PT2035556E (ru)
SI (1) SI2035556T1 (ru)
UA (1) UA97246C2 (ru)
WO (1) WO2007144475A1 (ru)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2327776A1 (en) 2009-11-30 2011-06-01 Institut National De La Recherche Agronomique Method for the production of Very Long Chain Fatty Acids (VLCFA) by fermentation with a recombinant Yarrowia sp
US20130295605A1 (en) * 2010-05-28 2013-11-07 Csir Method for producing a polypeptide in yarrowia lipolytica
CN102533692A (zh) * 2010-12-08 2012-07-04 刘刚 一种酸性条件下具有降解脂肪能力的脂肪酶
CN106754446B (zh) * 2016-10-31 2020-05-19 华中科技大学 一种转化解脂耶氏酵母及其构建方法与应用
AU2020336468A1 (en) 2019-08-30 2022-03-03 Societe Des Produits Nestle S.A. Engineered lipase variants
FR3111559A1 (fr) * 2020-06-18 2021-12-24 Azurrx Biopharma, Inc. Formulations non porcines et leurs procédés
KR102510688B1 (ko) 2022-06-15 2023-03-17 아스티스(주) 야로위아 리폴리티카 변이균주 및 이를 포함하는 기능성 화장료 조성물

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0706562A4 (en) * 1993-07-01 2000-12-06 Merck & Co Inc CULTURE MEDIUM FOR RECOMBINANT YEAST
PT1276874E (pt) 2000-04-28 2008-01-11 Mayoly Spindler Lab Clonagem e expressão de uma lipase extracelular resistente aos ácidos de yarrowia lipolytica
CN101084307B (zh) * 2004-09-30 2011-06-01 诺维信股份有限公司 具有脂肪酶活性的多肽和编码其的多核苷酸

Also Published As

Publication number Publication date
BRPI0621829A2 (pt) 2011-12-20
NO20085119L (no) 2009-03-02
KR101497159B1 (ko) 2015-03-03
EP2035556B1 (fr) 2015-07-29
CU20080242A7 (es) 2012-06-21
EP2035556A1 (fr) 2009-03-18
US20120003716A1 (en) 2012-01-05
ECSP099057A (es) 2009-08-28
AU2006344466B2 (en) 2013-08-15
CN101506359B (zh) 2013-09-04
DK2035556T3 (en) 2015-11-09
WO2007144475A1 (fr) 2007-12-21
MX2008015978A (es) 2009-03-26
HK1129702A1 (zh) 2009-12-04
US8334130B2 (en) 2012-12-18
SI2035556T1 (sl) 2015-12-31
ES2550537T3 (es) 2015-11-10
US8834867B2 (en) 2014-09-16
BRPI0621829B1 (pt) 2018-04-10
US20130052180A1 (en) 2013-02-28
PL2035556T3 (pl) 2015-12-31
AU2006344466A1 (en) 2007-12-21
KR20090029808A (ko) 2009-03-23
NZ573601A (en) 2011-12-22
CR10515A (es) 2009-04-21
BRPI0621829B8 (pt) 2021-05-25
CU24009B1 (es) 2014-06-27
CA2657949C (fr) 2014-07-15
JP2009539395A (ja) 2009-11-19
UA97246C2 (ru) 2012-01-25
PT2035556E (pt) 2015-11-04
NO342291B1 (no) 2018-04-30
EA017963B1 (ru) 2013-04-30
JP5592648B2 (ja) 2014-09-17
CA2657949A1 (fr) 2007-12-21
CN101506359A (zh) 2009-08-12

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