CN1961081B - 用于定量微rna和小干扰rna的新方法 - Google Patents

用于定量微rna和小干扰rna的新方法 Download PDF

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CN1961081B
CN1961081B CN2005800176883A CN200580017688A CN1961081B CN 1961081 B CN1961081 B CN 1961081B CN 2005800176883 A CN2005800176883 A CN 2005800176883A CN 200580017688 A CN200580017688 A CN 200580017688A CN 1961081 B CN1961081 B CN 1961081B
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sequence
probe
rna
template
lna
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CN1961081A (zh
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N·雅各布森
L·孔斯巴克
S·考皮南
S·M·埃克瓦尔德
P·莫里森
P·S·尼尔森
M·诺霍尔姆
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Qiagen GmbH
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Exiqon AS
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN2005800176883A 2004-04-07 2005-04-07 用于定量微rna和小干扰rna的新方法 Expired - Lifetime CN1961081B (zh)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
DKPA200400578 2004-04-07
DKPA200400578 2004-04-07
DKPA200401146 2004-07-23
DKPA200401146 2004-07-23
DKPA200401218 2004-08-11
DKPA200401218 2004-08-11
DKPA200401587 2004-10-15
DKPA200401587 2004-10-15
DKPA200500140 2005-01-28
DKPA200500140 2005-01-28
PCT/DK2005/000239 WO2005098029A2 (en) 2004-04-07 2005-04-07 Methods for quantification of micrornas and small interfering rnas

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CN1961081A CN1961081A (zh) 2007-05-09
CN1961081B true CN1961081B (zh) 2012-09-05

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EP (1) EP1735459B1 (OSRAM)
JP (1) JP5192229B2 (OSRAM)
CN (1) CN1961081B (OSRAM)
AT (1) ATE542918T1 (OSRAM)
CA (1) CA2562390C (OSRAM)
DK (1) DK1735459T3 (OSRAM)
ES (1) ES2380923T3 (OSRAM)
WO (1) WO2005098029A2 (OSRAM)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
EP1959012A3 (en) * 2004-12-29 2009-12-30 Exiqon A/S Novel oligonucleotide compositions and probe sequences useful for detection and analysis of microRNAs and their target mRNAs
ATE480644T1 (de) * 2005-01-25 2010-09-15 Rosetta Inpharmatics Llc Verfahren zur quantifizierung kleiner rna- moleküle
US8071306B2 (en) 2005-01-25 2011-12-06 Merck Sharp & Dohme Corp. Methods for quantitating small RNA molecules
US7556943B2 (en) 2005-05-31 2009-07-07 Applied Biosystems, Llc Linear amplification of short nucleic acids
JP2007082436A (ja) * 2005-09-20 2007-04-05 Bioinformatics Institute For Global Good Inc 機能性RNAが制御するターゲットmRNAの予測・同定方法及びその利用方法
EP1966390A1 (en) * 2005-12-29 2008-09-10 Exiqon A/S Detection of tissue origin of cancer
US8207325B2 (en) 2006-04-03 2012-06-26 Univ. of Copenhagen MicroRNA biomarkers for human breast and lung cancer
ES2569558T3 (es) 2006-04-03 2016-05-11 Roche Innovation Center Copenhagen A/S Composición farmacéutica que comprende oligonucleótidos antisentido anti-ARNmi
US7955848B2 (en) 2006-04-03 2011-06-07 Trustees Of Dartmouth College MicroRNA biomarkers for human breast and lung cancer
MX2008012219A (es) 2006-04-03 2008-10-02 Santaris Pharma As Composicion farmaceutica que comprende oligonucleotidos antisentido anti-miarn.
US8580494B2 (en) * 2006-08-25 2013-11-12 Research Foundation For Mental Hygiene, Inc. Methods and compositions for amplification and detection of MicroRNAs
WO2008040355A2 (en) * 2006-10-06 2008-04-10 Exiqon A/S Novel methods for quantification of micrornas and small interfering rnas
US8188255B2 (en) 2006-10-20 2012-05-29 Exiqon A/S Human microRNAs associated with cancer
EP2090665A2 (en) 2006-10-20 2009-08-19 Exiqon A/S Novel human microRNAs associated with cancer
EP1914317A1 (en) * 2006-10-21 2008-04-23 Biolytix AG A method for qualitative and quantitative detection of short nucleic acid sequences of about 8 to 50 nucleotides in length
US7645578B2 (en) 2006-10-31 2010-01-12 Agilent Technologies, Inc. Cleavage of RNA at redundant sites
WO2008113832A2 (en) 2007-03-22 2008-09-25 Santaris Pharma A/S SHORT RNA ANTAGONIST COMPOUNDS FOR THE MODULATION OF TARGET mRNA
US8470791B2 (en) 2007-03-22 2013-06-25 Santaris Pharma A/S RNA antagonist compounds for the inhibition of Apo-B100 expression
EP1978104A1 (en) * 2007-04-03 2008-10-08 Freie Universität Berlin A method for the quantitative analysis of RNA molecules in a sample
AU2008306327B2 (en) 2007-10-04 2014-05-15 Roche Innovation Center Copenhagen A/S Micromirs
AU2008310955B2 (en) * 2007-10-09 2015-01-29 Igor Vassily Kutyavin Methods, compositions and kits for the improved detection of small RNA molecules
JP5654352B2 (ja) 2007-11-09 2015-01-14 ボード・オブ・リージエンツ,ザ・ユニバーシテイ・オブ・テキサス・システム miR−15ファミリーのマイクロRNAによる心筋細胞生存及び心臓修復の調節
WO2009098988A1 (ja) * 2008-02-04 2009-08-13 Genecare Research Institute Co., Ltd. siRNA検出方法
US8404659B2 (en) 2008-03-07 2013-03-26 Santaris Pharma A/S Pharmaceutical compositions for treatment of MicroRNA related diseases
ES2541442T3 (es) 2008-08-01 2015-07-20 Roche Innovation Center Copenhagen A/S Modulación mediada por microARN de factores estimulantes de colonias
US20100240049A1 (en) 2009-01-16 2010-09-23 Cepheid Methods of Detecting Cervical Cancer
KR20110138341A (ko) * 2009-01-19 2011-12-27 시스테믹 스코틀랜드 리미티드 비암호화 rna 발현 검정법을 이용한 방법
DK2391736T3 (en) * 2009-02-02 2015-05-11 Exiqon As A method for the quantitation of small RNA species
AU2010207975B2 (en) 2009-02-02 2016-04-28 Cepheid Methods of detecting sepsis
US8962253B2 (en) 2009-04-13 2015-02-24 Somagenics Inc. Methods and compositions for detection of small RNAs
WO2010122538A1 (en) 2009-04-24 2010-10-28 Santaris Pharma A/S Pharmaceutical compositions for treatment of hcv patients that are non-responders to interferon
EP2456870A1 (en) 2009-07-21 2012-05-30 Santaris Pharma A/S Antisense oligomers targeting pcsk9
KR101822609B1 (ko) * 2010-06-24 2018-01-26 주식회사 파나진 Pna 기반의 실시간 pcr 클램핑을 이용한 마이크로rna 발현양상의 분석 방법 및 키트
US9200314B2 (en) 2010-07-21 2015-12-01 Takara Bio Inc. Synthetic siRNA detection method
JP2014513521A (ja) 2011-01-26 2014-06-05 セファイド 肺癌を検出する方法
CN102154505B (zh) * 2011-04-20 2013-03-20 苟德明 一种检测miRNA的方法及引物及其应用
WO2013096839A1 (en) 2011-12-22 2013-06-27 Somagenics, Inc. Methods of constructing small rna libraries and their use for expression profiling of target rnas
JP2015507928A (ja) 2012-02-14 2015-03-16 ザ・ジョンズ・ホプキンス・ユニバーシティ Mirna解析方法
US9163235B2 (en) 2012-06-21 2015-10-20 MiRagen Therapeutics, Inc. Inhibitors of the miR-15 family of micro-RNAs
EP3628746B1 (en) 2012-11-02 2025-08-27 Life Technologies Corporation Small rna capture, detection and quantification
US9862987B2 (en) 2013-01-16 2018-01-09 The Regents Of The University Of California Label free molecular detection methods, systems and devices
WO2014113598A2 (en) 2013-01-16 2014-07-24 The Regents Of The University Of California Microfluidic devices to extract, concentrate and isolate molecules
US10174352B2 (en) * 2013-03-14 2019-01-08 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids on solid support
CN103218543B (zh) * 2013-03-27 2016-04-13 中国科学院计算技术研究所 一种区分蛋白编码基因和非编码基因的方法及系统
MX391977B (es) 2013-06-27 2025-03-21 Roche Innovation Ct Copenhagen As Oligómeros antisentido y conjugados con diana en la proteína-convertasa subtilisina/kexina tipo 9(pcsk9).
CN104109708B (zh) * 2013-12-31 2015-05-13 厦门成坤生物技术有限公司 一种直接测定生物样品中未经分离的小核酸的方法及检测试剂盒
EP2942400A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
CN107223159A (zh) 2014-05-09 2017-09-29 科戴克斯生命股份公司 源自特定细胞类型的dna的检测及相关方法
ES2904909T3 (es) * 2014-10-08 2022-04-06 Univ Cornell Método para la identificación y la cuantificación relativa de cambios en la expresión de secuencias de ácidos nucleicos, en variantes de corte y empalme, en la translocación, en el número de copias o en la metilación utilizando reacciones combinadas de nucleasa, de ligadura y de polimerasa con prevención de arrastre
CN104598775B (zh) * 2014-12-31 2018-10-16 北京邮电大学 一种rna编辑事件识别方法
JP6383015B2 (ja) 2015-01-22 2018-08-29 アークレイ株式会社 ターゲット分析チップおよびターゲット分析方法
CN104673901B (zh) * 2015-02-06 2018-05-11 中国医学科学院基础医学研究所 用于检测靶核酸的用途和试剂盒
WO2016149021A1 (en) 2015-03-13 2016-09-22 Life Technologies Corporation Methods, compositions and kits for small rna capture, detection and quantification
CN104975078A (zh) * 2015-05-18 2015-10-14 上海市农业科学院 一种检测转基因植物中靶标siRNA池的方法
TWI676679B (zh) * 2015-06-02 2019-11-11 奎克生技光電股份有限公司 核苷酸序列、通用反向引子、通用反轉錄引子、引子設計方法及miRNA檢測方法
EP3168309B8 (en) 2015-11-10 2020-06-03 Eurofins LifeCodexx GmbH Detection of foetal chromosomal aneuploidies using dna regions that are differentially methylated between the foetus and the pregnant female
US11014957B2 (en) 2015-12-21 2021-05-25 Realseq Biosciences, Inc. Methods of library construction for polynucleotide sequencing
JP6577660B2 (ja) 2016-03-18 2019-09-18 株式会社東芝 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法
CZ2016248A3 (cs) * 2016-04-29 2017-12-20 Ústav molekulární genetiky AV ČR, v.v.i. Způsob detekce specifických mikroRNA pomocí templátové DNA a molekula templátové DNA pro použití při tomto způsobu
WO2019011875A1 (en) * 2017-07-11 2019-01-17 Qiagen Gmbh QUANTIFICATION OF ANTISENSE OLIGONUCLEOTIDES (ASOS)
WO2020127620A1 (en) * 2018-12-20 2020-06-25 F. Hoffmann-La Roche Ag Detection of target nucleic acid by solid-phase molography
WO2020239822A1 (en) * 2019-05-27 2020-12-03 The European Molecular Biology Laboratory Nucleic acid construct binding to influenza polymerase pb1 rna synthesis active site
US20220389497A1 (en) * 2019-11-04 2022-12-08 Mirnax Biosens, S.L. Bivalent reverse primer
EP3901286A1 (en) * 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
CN111665351B (zh) * 2020-06-20 2021-12-03 江南大学 一种快速、特异性测定rna含量的方法
CN113322523B (zh) * 2021-06-17 2024-03-19 翌圣生物科技(上海)股份有限公司 Rna快速建库方法及其应用
CN116200492B (zh) * 2022-11-08 2025-04-01 浙江大学 一种基于探针锚定双链的小核酸检测试剂盒及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001006004A2 (en) * 1999-07-19 2001-01-25 Cambridge University Technical Services Ltd. A method for amplifying low abundance nucleic acid sequences and means for performing said method
CA2433330A1 (en) * 2000-12-27 2002-07-25 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US7790867B2 (en) * 2002-12-05 2010-09-07 Rosetta Genomics Inc. Vaccinia virus-related nucleic acids and microRNA
WO2004057017A2 (en) * 2002-12-18 2004-07-08 Third Wave Technologies, Inc. Detection of small nucleic acids
AU2004254636A1 (en) * 2003-07-02 2005-01-13 Perkinelmer Las, Inc. Assay and process for labeling and detection of micro RNA and small interfering RNA sequences
US20080045417A1 (en) * 2003-10-14 2008-02-21 Jan Weiler Oligonucleotide Microarray

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Grad et al.Computational and Experimenttal Identification of C.elegans microRNAs.《Molecular Cell》.Call Press,2003,第11卷(第5期),1253-1263. *

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Publication number Publication date
ES2380923T3 (es) 2012-05-21
DK1735459T3 (da) 2012-05-29
EP1735459B1 (en) 2012-01-25
WO2005098029A3 (en) 2005-12-15
CN1961081A (zh) 2007-05-09
WO2005098029A2 (en) 2005-10-20
ATE542918T1 (de) 2012-02-15
EP1735459A2 (en) 2006-12-27
CA2562390C (en) 2014-12-02
CA2562390A1 (en) 2005-10-20
JP2007532100A (ja) 2007-11-15
JP5192229B2 (ja) 2013-05-08

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