DK1735459T3 - Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er - Google Patents
Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'erInfo
- Publication number
- DK1735459T3 DK1735459T3 DK05715157.3T DK05715157T DK1735459T3 DK 1735459 T3 DK1735459 T3 DK 1735459T3 DK 05715157 T DK05715157 T DK 05715157T DK 1735459 T3 DK1735459 T3 DK 1735459T3
- Authority
- DK
- Denmark
- Prior art keywords
- methods
- quantification
- expression
- monitoring
- well
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200400578 | 2004-04-07 | ||
DKPA200401146 | 2004-07-23 | ||
DKPA200401218 | 2004-08-11 | ||
DKPA200401587 | 2004-10-15 | ||
DKPA200500140 | 2005-01-28 | ||
PCT/DK2005/000239 WO2005098029A2 (en) | 2004-04-07 | 2005-04-07 | Methods for quantification of micrornas and small interfering rnas |
Publications (1)
Publication Number | Publication Date |
---|---|
DK1735459T3 true DK1735459T3 (da) | 2012-05-29 |
Family
ID=35116021
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK05715157.3T DK1735459T3 (da) | 2004-04-07 | 2005-04-07 | Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1735459B1 (da) |
JP (1) | JP5192229B2 (da) |
CN (1) | CN1961081B (da) |
AT (1) | ATE542918T1 (da) |
CA (1) | CA2562390C (da) |
DK (1) | DK1735459T3 (da) |
ES (1) | ES2380923T3 (da) |
WO (1) | WO2005098029A2 (da) |
Families Citing this family (67)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040219565A1 (en) | 2002-10-21 | 2004-11-04 | Sakari Kauppinen | Oligonucleotides useful for detecting and analyzing nucleic acids of interest |
US8192937B2 (en) | 2004-04-07 | 2012-06-05 | Exiqon A/S | Methods for quantification of microRNAs and small interfering RNAs |
US20070099196A1 (en) * | 2004-12-29 | 2007-05-03 | Sakari Kauppinen | Novel oligonucleotide compositions and probe sequences useful for detection and analysis of micrornas and their target mRNAs |
US8071306B2 (en) | 2005-01-25 | 2011-12-06 | Merck Sharp & Dohme Corp. | Methods for quantitating small RNA molecules |
DE602006016739D1 (de) * | 2005-01-25 | 2010-10-21 | Rosetta Inpharmatics Llc | Verfahren zur quantifizierung kleiner rna-moleküle |
WO2006130761A1 (en) | 2005-05-31 | 2006-12-07 | Applera Corporation | Linear amplification of short nucleic acids |
JP2007082436A (ja) * | 2005-09-20 | 2007-04-05 | Bioinformatics Institute For Global Good Inc | 機能性RNAが制御するターゲットmRNAの予測・同定方法及びその利用方法 |
EP1966390A1 (en) * | 2005-12-29 | 2008-09-10 | Exiqon A/S | Detection of tissue origin of cancer |
JP5814505B2 (ja) | 2006-04-03 | 2015-11-17 | ロシュ・イノベーション・センター・コペンハーゲン・アクティーゼルスカブRoche Innovation Center Copenhagen A/S | antimiRNAアンチセンスオリゴヌクレオチドを含む医薬組成物 |
EP3431602A1 (en) | 2006-04-03 | 2019-01-23 | Roche Innovation Center Copenhagen A/S | Pharmaceutical composition comprising anti-mirna antisense oligonucleotides |
US8207325B2 (en) | 2006-04-03 | 2012-06-26 | Univ. of Copenhagen | MicroRNA biomarkers for human breast and lung cancer |
US7955848B2 (en) | 2006-04-03 | 2011-06-07 | Trustees Of Dartmouth College | MicroRNA biomarkers for human breast and lung cancer |
CN101082060B (zh) * | 2006-06-01 | 2012-09-05 | 苏州吉玛基因药物科技有限公司 | 新型微核糖核酸定量pcr(聚合酶链式反应)检测方法 |
US8580494B2 (en) | 2006-08-25 | 2013-11-12 | Research Foundation For Mental Hygiene, Inc. | Methods and compositions for amplification and detection of MicroRNAs |
WO2008040355A2 (en) * | 2006-10-06 | 2008-04-10 | Exiqon A/S | Novel methods for quantification of micrornas and small interfering rnas |
US8188255B2 (en) | 2006-10-20 | 2012-05-29 | Exiqon A/S | Human microRNAs associated with cancer |
WO2008046911A2 (en) | 2006-10-20 | 2008-04-24 | Exiqon A/S | Novel human micrornas associated with cancer |
EP1914317A1 (en) * | 2006-10-21 | 2008-04-23 | Biolytix AG | A method for qualitative and quantitative detection of short nucleic acid sequences of about 8 to 50 nucleotides in length |
US7645578B2 (en) | 2006-10-31 | 2010-01-12 | Agilent Technologies, Inc. | Cleavage of RNA at redundant sites |
AU2008228243B2 (en) | 2007-03-22 | 2014-05-15 | Santaris Pharma A/S | RNA antagonist compounds for the inhibition of Apo-B100 expression |
DK2149605T3 (da) | 2007-03-22 | 2013-09-30 | Santaris Pharma As | Korte RNA antagonist forbindelser til modulering af det ønskede mRNA |
EP1978104A1 (en) * | 2007-04-03 | 2008-10-08 | Freie Universität Berlin | A method for the quantitative analysis of RNA molecules in a sample |
AU2008306327B2 (en) | 2007-10-04 | 2014-05-15 | Roche Innovation Center Copenhagen A/S | Micromirs |
CA2702584A1 (en) * | 2007-10-09 | 2009-04-16 | Igor Vassily Kutyavin | Methods, compositions and kits for the improved detection of small rna molecules |
KR20100099158A (ko) | 2007-11-09 | 2010-09-10 | 보드 오브 리전츠 더 유니버시티 오브 텍사스 시스템 | 심근세포 생존과 심장 회복을 제어하는 MIR-15 집단의 마이크로RNAs |
WO2009098988A1 (ja) | 2008-02-04 | 2009-08-13 | Genecare Research Institute Co., Ltd. | siRNA検出方法 |
US8404659B2 (en) | 2008-03-07 | 2013-03-26 | Santaris Pharma A/S | Pharmaceutical compositions for treatment of MicroRNA related diseases |
ES2541442T3 (es) | 2008-08-01 | 2015-07-20 | Roche Innovation Center Copenhagen A/S | Modulación mediada por microARN de factores estimulantes de colonias |
US20100240049A1 (en) | 2009-01-16 | 2010-09-23 | Cepheid | Methods of Detecting Cervical Cancer |
JP2012514994A (ja) * | 2009-01-19 | 2012-07-05 | システミック・スコットランド・リミテッド | 非コードrna発現アッセイを用いた方法 |
ES2541356T3 (es) * | 2009-02-02 | 2015-07-17 | Exiqon A/S | Procedimiento de cuantificación de especies pequeñas de ARN |
WO2010088668A2 (en) | 2009-02-02 | 2010-08-05 | Cepheid | Methods of detecting sepsis |
EP2419537B1 (en) | 2009-04-13 | 2014-01-29 | Somagenics, Inc. | Methods and compositions for detection of small rnas |
WO2010122538A1 (en) | 2009-04-24 | 2010-10-28 | Santaris Pharma A/S | Pharmaceutical compositions for treatment of hcv patients that are non-responders to interferon |
US8563528B2 (en) | 2009-07-21 | 2013-10-22 | Santaris Pharma A/S | Antisense oligomers targeting PCSK9 |
KR101822609B1 (ko) * | 2010-06-24 | 2018-01-26 | 주식회사 파나진 | Pna 기반의 실시간 pcr 클램핑을 이용한 마이크로rna 발현양상의 분석 방법 및 키트 |
US9200314B2 (en) * | 2010-07-21 | 2015-12-01 | Takara Bio Inc. | Synthetic siRNA detection method |
CA2825522A1 (en) | 2011-01-26 | 2012-08-02 | Cepheid | Methods of detecting lung cancer |
CN102154505B (zh) * | 2011-04-20 | 2013-03-20 | 苟德明 | 一种检测miRNA的方法及引物及其应用 |
WO2013096839A1 (en) | 2011-12-22 | 2013-06-27 | Somagenics, Inc. | Methods of constructing small rna libraries and their use for expression profiling of target rnas |
EP2814984A4 (en) | 2012-02-14 | 2015-07-29 | Univ Johns Hopkins | MIRNA ANALYSIS PROCEDURE |
US9163235B2 (en) | 2012-06-21 | 2015-10-20 | MiRagen Therapeutics, Inc. | Inhibitors of the miR-15 family of micro-RNAs |
CN111793621A (zh) | 2012-11-02 | 2020-10-20 | 生命技术公司 | 用于增强pcr特异性的新颖组合物、方法和试剂盒 |
WO2014113614A1 (en) * | 2013-01-16 | 2014-07-24 | The Regents Of The University Of California | Label free molecular detection methods, systems and devices |
WO2014113598A2 (en) | 2013-01-16 | 2014-07-24 | The Regents Of The University Of California | Microfluidic devices to extract, concentrate and isolate molecules |
JP6479759B2 (ja) * | 2013-03-14 | 2019-03-06 | アーノルド, ライル, ジェイ.ARNOLD, Lyle, J. | 固体支持体上での核酸増幅方法 |
CN103218543B (zh) * | 2013-03-27 | 2016-04-13 | 中国科学院计算技术研究所 | 一种区分蛋白编码基因和非编码基因的方法及系统 |
CN112263682A (zh) | 2013-06-27 | 2021-01-26 | 罗氏创新中心哥本哈根有限公司 | 靶向pcsk9的反义寡聚体和缀合物 |
CN104109708B (zh) * | 2013-12-31 | 2015-05-13 | 厦门成坤生物技术有限公司 | 一种直接测定生物样品中未经分离的小核酸的方法及检测试剂盒 |
EP2942400A1 (en) | 2014-05-09 | 2015-11-11 | Lifecodexx AG | Multiplex detection of DNA that originates from a specific cell-type |
EP3140421B8 (en) | 2014-05-09 | 2020-05-06 | Eurofins LifeCodexx GmbH | Detection of dna that originates from a specific cell-type and related methods |
CN104598775B (zh) * | 2014-12-31 | 2018-10-16 | 北京邮电大学 | 一种rna编辑事件识别方法 |
JP6383015B2 (ja) | 2015-01-22 | 2018-08-29 | アークレイ株式会社 | ターゲット分析チップおよびターゲット分析方法 |
CN104673901B (zh) * | 2015-02-06 | 2018-05-11 | 中国医学科学院基础医学研究所 | 用于检测靶核酸的用途和试剂盒 |
WO2016149021A1 (en) | 2015-03-13 | 2016-09-22 | Life Technologies Corporation | Methods, compositions and kits for small rna capture, detection and quantification |
CN104975078A (zh) * | 2015-05-18 | 2015-10-14 | 上海市农业科学院 | 一种检测转基因植物中靶标siRNA池的方法 |
TWI676679B (zh) * | 2015-06-02 | 2019-11-11 | 奎克生技光電股份有限公司 | 核苷酸序列、通用反向引子、通用反轉錄引子、引子設計方法及miRNA檢測方法 |
HUE050491T2 (hu) | 2015-11-10 | 2020-12-28 | Eurofins Lifecodexx Gmbh | Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között |
US11014957B2 (en) | 2015-12-21 | 2021-05-25 | Realseq Biosciences, Inc. | Methods of library construction for polynucleotide sequencing |
WO2017158840A1 (ja) | 2016-03-18 | 2017-09-21 | 株式会社 東芝 | 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法 |
CZ2016248A3 (cs) * | 2016-04-29 | 2017-12-20 | Ústav molekulární genetiky AV ČR, v.v.i. | Způsob detekce specifických mikroRNA pomocí templátové DNA a molekula templátové DNA pro použití při tomto způsobu |
WO2019011875A1 (en) * | 2017-07-11 | 2019-01-17 | Qiagen Gmbh | QUANTIFICATION OF ANTISENSE OLIGONUCLEOTIDES (ASOS) |
WO2020239822A1 (en) * | 2019-05-27 | 2020-12-03 | The European Molecular Biology Laboratory | Nucleic acid construct binding to influenza polymerase pb1 rna synthesis active site |
EP3901286A1 (en) * | 2020-04-24 | 2021-10-27 | Mirnax Biosens, S.L. | Bivalent reverse primer |
US20220389497A1 (en) * | 2019-11-04 | 2022-12-08 | Mirnax Biosens, S.L. | Bivalent reverse primer |
CN111665351B (zh) * | 2020-06-20 | 2021-12-03 | 江南大学 | 一种快速、特异性测定rna含量的方法 |
CN113322523B (zh) * | 2021-06-17 | 2024-03-19 | 翌圣生物科技(上海)股份有限公司 | Rna快速建库方法及其应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20020034161A (ko) * | 1999-07-19 | 2002-05-08 | 알. 씨. 제닝스 | 불충분한 핵산 서열의 증폭방법 및 그 방법의 수행장치 |
JP2004532615A (ja) * | 2000-12-27 | 2004-10-28 | インヴィトロジェン コーポレーション | 核酸の検出および識別のためのプライマーおよび方法 |
US20040235005A1 (en) * | 2002-10-23 | 2004-11-25 | Ernest Friedlander | Methods and composition for detecting targets |
US7790867B2 (en) * | 2002-12-05 | 2010-09-07 | Rosetta Genomics Inc. | Vaccinia virus-related nucleic acids and microRNA |
AU2003297541A1 (en) * | 2002-12-18 | 2004-07-14 | Third Wave Technologies, Inc. | Detection of small nucleic acids |
EP1644533A4 (en) * | 2003-07-02 | 2007-11-14 | Perkinelmer Las Inc | ANALYSIS AND METHOD OF MARKING AND DETECTING RNA MICROSEQUENCES AND SMALL INTERFERENCE RNA SEQUENCES |
EP1675965A1 (en) * | 2003-10-14 | 2006-07-05 | Novartis AG | Oligonucleotide microarray |
-
2005
- 2005-04-07 EP EP05715157A patent/EP1735459B1/en active Active
- 2005-04-07 CA CA2562390A patent/CA2562390C/en active Active
- 2005-04-07 ES ES05715157T patent/ES2380923T3/es active Active
- 2005-04-07 DK DK05715157.3T patent/DK1735459T3/da active
- 2005-04-07 CN CN2005800176883A patent/CN1961081B/zh active Active
- 2005-04-07 AT AT05715157T patent/ATE542918T1/de active
- 2005-04-07 WO PCT/DK2005/000239 patent/WO2005098029A2/en active Application Filing
- 2005-04-07 JP JP2007506659A patent/JP5192229B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
ES2380923T3 (es) | 2012-05-21 |
ATE542918T1 (de) | 2012-02-15 |
WO2005098029A3 (en) | 2005-12-15 |
CN1961081B (zh) | 2012-09-05 |
EP1735459B1 (en) | 2012-01-25 |
JP5192229B2 (ja) | 2013-05-08 |
CN1961081A (zh) | 2007-05-09 |
CA2562390A1 (en) | 2005-10-20 |
CA2562390C (en) | 2014-12-02 |
EP1735459A2 (en) | 2006-12-27 |
JP2007532100A (ja) | 2007-11-15 |
WO2005098029A2 (en) | 2005-10-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK1735459T3 (da) | Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er | |
WO2008040355A3 (en) | Novel methods for quantification of micrornas and small interfering rnas | |
Yoshimoto et al. | MALAT1 long non-coding RNA in cancer | |
Beaudoin et al. | Exploring mRNA 3′-UTR G-quadruplexes: evidence of roles in both alternative polyadenylation and mRNA shortening | |
Ichii et al. | Altered expression of microRNA miR-146a correlates with the development of chronic renal inflammation | |
Lopez et al. | Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing | |
DK1597391T3 (da) | Anvendelse af intron-RNA til måling af genekspression | |
Friedman et al. | Most mammalian mRNAs are conserved targets of microRNAs | |
Wu et al. | Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells | |
WO2006069584A3 (en) | NOVEL OLIGONUCLEOTIDE COMPOSITIONS AND PROBE SEQUENCES USEFUL FOR DETECTION AND ANALYSIS OF microRNAs AND THEIR TARGET mRNAs | |
WO2009152928A3 (de) | Verfahren zur quantitativen analyse von nukleinsäuren, marker dafür und deren verwendung | |
Badal et al. | MicroRNAs and their applications in kidney diseases | |
WO2006119266A3 (en) | Human micrornas and methods for inhibiting same | |
ATE411400T1 (de) | Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen | |
ATE437962T1 (de) | Verfahren für die behandlung von proliferativen erkrankungen von brustzellen | |
Clarke et al. | Circulating microRNA 122 in the methionine and choline‐deficient mouse model of non‐alcoholic steatohepatitis | |
DK1730315T3 (da) | Polymorphismer i NOD2/Card15 gen | |
WO2004111190A3 (en) | IN VIVO HIGH THROUGHPUT SELECTION OF EFFECTIVE RNAi PROBES | |
Chen et al. | Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation | |
WO2006012468A3 (en) | Detection of oligonuleotides by dual hybridization | |
WO2008094516A8 (en) | Multi-targeting short interfering rnas | |
ATE528008T1 (de) | Rnai-modulation von aha und ihre therapeutische verwendung | |
ATE505562T1 (de) | Doppel-oligonukleotid- nukleinsäurenachweisverfahren | |
MX2009002462A (es) | Siarn y metodos de manufactura del mismo. | |
Meister et al. | MicroRNAs in the hypothalamus |