CN1468251B - 一种用于均相检测核酸的特异性双链探针及其实施方法 - Google Patents
一种用于均相检测核酸的特异性双链探针及其实施方法 Download PDFInfo
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- CN1468251B CN1468251B CN018170129A CN01817012A CN1468251B CN 1468251 B CN1468251 B CN 1468251B CN 018170129 A CN018170129 A CN 018170129A CN 01817012 A CN01817012 A CN 01817012A CN 1468251 B CN1468251 B CN 1468251B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
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- Biotechnology (AREA)
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- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract
Description
Claims (20)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN018170129A CN1468251B (zh) | 2000-10-10 | 2001-10-05 | 一种用于均相检测核酸的特异性双链探针及其实施方法 |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN00130711.8 | 2000-10-10 | ||
CN00130711 | 2000-10-10 | ||
CN01101446.6 | 2001-01-13 | ||
CN01101446A CN1348096A (zh) | 2000-10-10 | 2001-01-13 | 一种均相特异性检测核酸的探针及应用方法 |
CN018170129A CN1468251B (zh) | 2000-10-10 | 2001-10-05 | 一种用于均相检测核酸的特异性双链探针及其实施方法 |
PCT/US2001/031246 WO2002030946A1 (en) | 2000-10-10 | 2001-10-05 | Specific double-stranded probes for homogeneous detection of nucleic acid and their application methods |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1468251A CN1468251A (zh) | 2004-01-14 |
CN1468251B true CN1468251B (zh) | 2012-11-21 |
Family
ID=25739622
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN01101446A Pending CN1348096A (zh) | 2000-10-10 | 2001-01-13 | 一种均相特异性检测核酸的探针及应用方法 |
CN018170129A Expired - Fee Related CN1468251B (zh) | 2000-10-10 | 2001-10-05 | 一种用于均相检测核酸的特异性双链探针及其实施方法 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN01101446A Pending CN1348096A (zh) | 2000-10-10 | 2001-01-13 | 一种均相特异性检测核酸的探针及应用方法 |
Country Status (8)
Country | Link |
---|---|
US (2) | US7799522B2 (zh) |
EP (1) | EP1339732B1 (zh) |
JP (1) | JP3999653B2 (zh) |
CN (2) | CN1348096A (zh) |
AU (2) | AU2001296647B2 (zh) |
BR (1) | BR0114575A (zh) |
CA (1) | CA2424856C (zh) |
WO (1) | WO2002030946A1 (zh) |
Families Citing this family (50)
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CN1348096A (zh) * | 2000-10-10 | 2002-05-08 | 栾国彦 | 一种均相特异性检测核酸的探针及应用方法 |
US9261460B2 (en) * | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
CN1294276C (zh) * | 2003-07-22 | 2007-01-10 | 厦门大学 | 检测核酸的等长双链特异性探针 |
CN1860241A (zh) * | 2003-07-30 | 2006-11-08 | 财团法人理工学振兴会 | 部分双链型核酸分子 |
WO2005061734A2 (en) * | 2003-12-03 | 2005-07-07 | Abbott Laboratories | Double stranded linear nucleic acid probe and uses thereof |
US20050260624A1 (en) * | 2004-02-28 | 2005-11-24 | Wang Chang N J | Novel nucleic acid complexes and detection thereof |
DE102004026494A1 (de) * | 2004-05-27 | 2005-12-22 | Ruprecht-Karls-Universität Heidelberg | Hochselektiver Nachweis von DNA-Molekülen mit gemischten PNA-DNA-Systemen |
JP2008501679A (ja) * | 2004-06-02 | 2008-01-24 | エーエスエム サイエンティフィック, インコーポレイテッド | 2’−ニトロベンジル改変型リボヌクレオチド |
GB0419325D0 (en) * | 2004-09-01 | 2004-09-29 | Perkinelmer Ltd | A method of analysing a sample including fluorescent labels and apparatus therefor |
GB0510979D0 (en) * | 2005-05-28 | 2005-07-06 | Kbiosciences Ltd | Detection system for PCR assay |
US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
US9133504B2 (en) | 2006-06-05 | 2015-09-15 | California Institute Of Technology | Real time microarrays |
US7972786B2 (en) * | 2006-07-07 | 2011-07-05 | Brandeis University | Detection and analysis of influenza virus |
US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
EP1911852B1 (en) * | 2006-10-12 | 2009-07-29 | Bio-Rad Pasteur | Double-stranded probes for the fluorescent detection of nucleic acids |
JP5013604B2 (ja) * | 2007-07-24 | 2012-08-29 | 株式会社スギヨ | 海藻ペーストの製造方法及びその方法によって得られた海藻ペースト |
WO2010130877A2 (en) * | 2009-05-12 | 2010-11-18 | Abacus Diagnostica Oy | Method for detecting nucleic acids |
CN101845498B (zh) * | 2010-04-29 | 2013-05-08 | 厦门艾德生物医药科技有限公司 | 一种用于核酸特异识别检测的探针 |
DE102010025161A1 (de) * | 2010-06-25 | 2011-12-29 | Qiagen Gmbh | Verfahren zum Nachweis von Ribonukleinsäuren |
US20120052502A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Real-time pcr detection using stabilized probes |
US9284602B2 (en) | 2010-10-27 | 2016-03-15 | President And Fellows Of Harvard College | Compositions of toehold primer duplexes and methods of use |
WO2012151328A2 (en) | 2011-05-02 | 2012-11-08 | President And Fellows Of Harvard College | Spatial sequestration of dynamic nucleic acid circuits |
CN108342453A (zh) | 2011-05-09 | 2018-07-31 | 富鲁达公司 | 基于探针的核酸检测 |
US9644231B2 (en) | 2011-05-09 | 2017-05-09 | Fluidigm Corporation | Nucleic acid detection using probes |
DE102011078342A1 (de) * | 2011-06-29 | 2013-01-03 | Aj Innuscreen Gmbh | Rehybridisierendes Sondensystem zur qualitativen und quantitativen Messung von spezifischen Nukleinsäuren in Echtzeit |
ES2755934T3 (es) * | 2011-10-31 | 2020-04-24 | Eiken Chemical | Método para detectar ácido nucleico diana |
MX2015001961A (es) * | 2012-08-16 | 2015-09-10 | Nvs Technologies Inc | Metodos y sistemas para ensayos. |
CN105378160B (zh) | 2013-08-12 | 2018-04-17 | 伯乐生命医学产品有限公司 | 具有碱基配对的寡聚体的扩增报告子 |
JP5928906B2 (ja) * | 2013-08-27 | 2016-06-01 | 横河電機株式会社 | 核酸配列計測方法、核酸配列計測用デバイス、核酸配列計測用デバイスの製造方法および核酸配列計測装置 |
CA2934226C (en) * | 2013-12-16 | 2022-12-06 | William Marsh Rice University | Fine-tuned ultraspecific nucleic acid hybridization probes |
US20180320223A1 (en) * | 2015-03-19 | 2018-11-08 | Quandx Inc. | Ided double-stranded probes for detection of nucleic acid and uses of same |
US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
CN105506126B (zh) * | 2016-01-12 | 2019-02-26 | 重庆大学 | 具有较大张力的小环形核苷酸探针及其制备方法和应用 |
EP3415636B1 (en) | 2016-02-09 | 2020-11-25 | Eiken Kagaku Kabushiki Kaisha | Method for detecting target nucleic acid and nucleic acid probe used therein |
WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
JP6584986B2 (ja) * | 2016-03-18 | 2019-10-02 | 株式会社東芝 | 核酸検出方法 |
WO2017216635A1 (en) | 2016-06-16 | 2017-12-21 | Bio-Rad Laboratories, Inc. | Method of detecting salmonella typhimurium |
GB201617491D0 (en) * | 2016-10-14 | 2016-11-30 | Moorlodge Biotech Ventures Limited | Amplification of nucleic acids |
CN107236815A (zh) * | 2017-07-18 | 2017-10-10 | 江西贤聚景欣医药生物科技有限公司 | 用于靶核酸序列检测的多重淬灭荧光探针及方法 |
KR101899371B1 (ko) * | 2017-07-25 | 2018-10-29 | (주)엔바이오텍 | 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법 |
JP7035972B2 (ja) * | 2018-11-09 | 2022-03-15 | 横河電機株式会社 | 核酸配列計測用デバイス |
CN109652516A (zh) * | 2018-12-29 | 2019-04-19 | 中国人民解放军军事科学院军事医学研究院 | 一种双链寡核苷酸核酸探针的结构和用途 |
EP3937780A4 (en) | 2019-03-14 | 2022-12-07 | InSilixa, Inc. | METHODS AND SYSTEMS FOR TIMED FLUORESCENCE-BASED DETECTION |
WO2022132955A2 (en) * | 2020-12-16 | 2022-06-23 | Proof Diagnostics, Inc. | Coronavirus rapid diagnostics |
WO2023023673A2 (en) | 2021-08-20 | 2023-02-23 | Kasa Bio, L.L.C. | Compositions and methods for multiplex detection of mirna and other polynucelotides |
EP4148143A1 (en) * | 2021-09-13 | 2023-03-15 | Consejo Superior De Investigaciones Científicas (CSIC) | Polypurine reverse hoogsteen hairpins and parallel clamps and their use as biosensors |
US20230313283A1 (en) * | 2022-02-28 | 2023-10-05 | Trustees Of Boston University | Isothermal nucleic acid detection assays and uses thereof |
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-
2001
- 2001-01-13 CN CN01101446A patent/CN1348096A/zh active Pending
- 2001-10-05 BR BR0114575-4A patent/BR0114575A/pt not_active IP Right Cessation
- 2001-10-05 AU AU2001296647A patent/AU2001296647B2/en not_active Ceased
- 2001-10-05 US US10/398,832 patent/US7799522B2/en not_active Expired - Lifetime
- 2001-10-05 AU AU9664701A patent/AU9664701A/xx active Pending
- 2001-10-05 WO PCT/US2001/031246 patent/WO2002030946A1/en active Application Filing
- 2001-10-05 JP JP2002534331A patent/JP3999653B2/ja not_active Expired - Fee Related
- 2001-10-05 CA CA2424856A patent/CA2424856C/en not_active Expired - Fee Related
- 2001-10-05 CN CN018170129A patent/CN1468251B/zh not_active Expired - Fee Related
- 2001-10-05 EP EP01977535A patent/EP1339732B1/en not_active Expired - Lifetime
-
2010
- 2010-08-11 US US12/854,619 patent/US9157112B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2424856C (en) | 2014-03-11 |
CA2424856A1 (en) | 2002-04-18 |
JP3999653B2 (ja) | 2007-10-31 |
AU2001296647B2 (en) | 2006-03-16 |
US7799522B2 (en) | 2010-09-21 |
EP1339732A1 (en) | 2003-09-03 |
EP1339732B1 (en) | 2012-08-01 |
CN1468251A (zh) | 2004-01-14 |
US20110129828A1 (en) | 2011-06-02 |
AU9664701A (en) | 2002-04-22 |
US20040023269A1 (en) | 2004-02-05 |
BR0114575A (pt) | 2004-01-06 |
JP2004511227A (ja) | 2004-04-15 |
US9157112B2 (en) | 2015-10-13 |
EP1339732A4 (en) | 2004-04-21 |
CN1348096A (zh) | 2002-05-08 |
WO2002030946A1 (en) | 2002-04-18 |
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