CN1370230A - Dna的同时分离和定量 - Google Patents

Dna的同时分离和定量 Download PDF

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Publication number
CN1370230A
CN1370230A CN00811856.6A CN00811856A CN1370230A CN 1370230 A CN1370230 A CN 1370230A CN 00811856 A CN00811856 A CN 00811856A CN 1370230 A CN1370230 A CN 1370230A
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China
Prior art keywords
target material
dna
dna target
mixture
particle
Prior art date
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Pending
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CN00811856.6A
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English (en)
Chinese (zh)
Inventor
艾伦·M·特雷巴
雷克斯·M·比特纳
苏珊·C·科勒
克雷格·E·史密斯
丹尼尔·D·凯法特
史蒂文·J·埃肯贝里
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Promega Corp
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Promega Corp
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Filing date
Publication date
Priority claimed from US09/377,986 external-priority patent/US6673631B1/en
Application filed by Promega Corp filed Critical Promega Corp
Publication of CN1370230A publication Critical patent/CN1370230A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN00811856.6A 1999-08-20 2000-08-18 Dna的同时分离和定量 Pending CN1370230A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/377,986 US6673631B1 (en) 1997-01-21 1999-08-20 Simultaneous isolation and quantitation of DNA
US09/377,986 1999-08-20

Publications (1)

Publication Number Publication Date
CN1370230A true CN1370230A (zh) 2002-09-18

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ID=23491269

Family Applications (1)

Application Number Title Priority Date Filing Date
CN00811856.6A Pending CN1370230A (zh) 1999-08-20 2000-08-18 Dna的同时分离和定量

Country Status (8)

Country Link
EP (1) EP1204741B1 (https=)
JP (1) JP4425513B2 (https=)
CN (1) CN1370230A (https=)
AT (1) ATE280225T1 (https=)
AU (1) AU780319B2 (https=)
CA (1) CA2379503A1 (https=)
DE (1) DE60015148T2 (https=)
WO (1) WO2001014590A2 (https=)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100531895C (zh) * 2004-07-29 2009-08-26 恰根有限公司 用于纯化和分离生物聚合物的单片成型体
CN101818143A (zh) * 2010-04-28 2010-09-01 长春市博坤生物科技有限公司 基于二氧化硅磁性复合微粒的超低量dna提取试剂盒及方法
CN102667481A (zh) * 2009-12-23 2012-09-12 皇家飞利浦电子股份有限公司 分析物测量设备和方法
CN102834518A (zh) * 2010-01-08 2012-12-19 霍夫曼-拉罗奇有限公司 改进的从磁性玻璃颗粒中的核酸回收
CN101010592B (zh) * 2004-07-26 2013-08-07 拜奥默里克斯有限公司 将磁性粒子与流体分离、混合和浓缩的设备和方法及其在提纯方法中的用途
CN104560952A (zh) * 2014-12-29 2015-04-29 福建师范大学 一种基于磁珠法快速提取革兰氏阳性菌基因组dna的方法
CN105603056A (zh) * 2014-11-21 2016-05-25 丰益(上海)生物技术研发中心有限公司 一种植物油脂掺杂检测的方法
CN109321634A (zh) * 2017-07-26 2019-02-12 上海之江生物科技股份有限公司 核酸均一化方法及其试剂盒和应用
CN110168366A (zh) * 2017-01-04 2019-08-23 新加坡科技研究局 用于基于颗粒的生物测定的过筛垂直流系统
CN110511923A (zh) * 2012-01-25 2019-11-29 基因细胞生物系统有限公司 生物分子分离

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003046146A2 (en) 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
WO2003057910A2 (en) 2002-01-08 2003-07-17 Roche Diagnostics Gmbh Use of silica material in an amplification reaction
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
EP1690938A1 (de) * 2005-02-11 2006-08-16 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden
US20090277790A1 (en) 2006-04-14 2009-11-12 Nec Corporation Individual identification method and apparatus
JP2009065900A (ja) * 2007-09-13 2009-04-02 Olympus Corp 標的ｒｎａの解析方法及び保存方法
JP2011504591A (ja) * 2007-11-26 2011-02-10 アトノミックス アクティーゼルスカブ 信号対ノイズ比を増すための手段および方法を備える、統合型分離および検出カートリッジ
DE102008047790A1 (de) * 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
DE112011102524T5 (de) 2010-07-29 2013-07-11 Roche Diagnostics Gmbh Präparation generischer Proben
US9725754B2 (en) 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
AU2013240200B2 (en) * 2012-03-28 2016-10-20 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for the collection and isolation of nucleic acids from biological specimens
WO2014035090A1 (ko) 2012-08-28 2014-03-06 주식회사 바이오큐브시스템 생물학적 시료로부터 핵산 증폭 반응용 생물학적 분자를 신속하게 분리하기 위한 다공성 고체상 및 이의 용도
EP3294448A4 (en) 2015-05-14 2018-12-12 Longhorn Vaccines and Diagnostics, LLC Rapid methods for the extraction of nucleic acids from biological samples
JP2018526021A (ja) * 2015-09-11 2018-09-13 コーニング インコーポレイテッド 血液試料からの核酸精製のための組成物及び方法
WO2019035335A1 (ja) * 2017-08-18 2019-02-21 Ａｇｃ株式会社 核酸の回収方法、核酸結合用担体および核酸回収キット
US20210260600A1 (en) * 2020-02-01 2021-08-26 Sunil Mehta Automated device and method to purify biomaterials from a mixture by using magnetic particles and disposable product-contact materials
EP4437127A1 (en) * 2021-11-23 2024-10-02 Bioo Scientific Corporation Compositions and methods for nucleic acid normalization

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101010592B (zh) * 2004-07-26 2013-08-07 拜奥默里克斯有限公司 将磁性粒子与流体分离、混合和浓缩的设备和方法及其在提纯方法中的用途
CN100531895C (zh) * 2004-07-29 2009-08-26 恰根有限公司 用于纯化和分离生物聚合物的单片成型体
CN102667481A (zh) * 2009-12-23 2012-09-12 皇家飞利浦电子股份有限公司 分析物测量设备和方法
CN102834518A (zh) * 2010-01-08 2012-12-19 霍夫曼-拉罗奇有限公司 改进的从磁性玻璃颗粒中的核酸回收
CN102834518B (zh) * 2010-01-08 2015-04-01 霍夫曼-拉罗奇有限公司 改进的从磁性玻璃颗粒中的核酸回收
CN101818143A (zh) * 2010-04-28 2010-09-01 长春市博坤生物科技有限公司 基于二氧化硅磁性复合微粒的超低量dna提取试剂盒及方法
CN110511923A (zh) * 2012-01-25 2019-11-29 基因细胞生物系统有限公司 生物分子分离
CN105603056A (zh) * 2014-11-21 2016-05-25 丰益(上海)生物技术研发中心有限公司 一种植物油脂掺杂检测的方法
CN105603056B (zh) * 2014-11-21 2020-09-25 丰益(上海)生物技术研发中心有限公司 一种植物油脂掺杂检测的方法
CN104560952A (zh) * 2014-12-29 2015-04-29 福建师范大学 一种基于磁珠法快速提取革兰氏阳性菌基因组dna的方法
CN110168366A (zh) * 2017-01-04 2019-08-23 新加坡科技研究局 用于基于颗粒的生物测定的过筛垂直流系统
CN109321634A (zh) * 2017-07-26 2019-02-12 上海之江生物科技股份有限公司 核酸均一化方法及其试剂盒和应用

Also Published As

Publication number Publication date
EP1204741B1 (en) 2004-10-20
ATE280225T1 (de) 2004-11-15
AU780319B2 (en) 2005-03-17
EP1204741A2 (en) 2002-05-15
DE60015148D1 (de) 2004-11-25
WO2001014590A3 (en) 2001-10-11
DE60015148T2 (de) 2006-02-16
CA2379503A1 (en) 2001-03-01
AU6789500A (en) 2001-03-19
WO2001014590A2 (en) 2001-03-01
JP4425513B2 (ja) 2010-03-03
JP2003507049A (ja) 2003-02-25

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