DE60015148D1 - Simultane isolierung und quantifizierung von dna - Google Patents

Simultane isolierung und quantifizierung von dna

Info

Publication number
DE60015148D1
DE60015148D1 DE60015148T DE60015148T DE60015148D1 DE 60015148 D1 DE60015148 D1 DE 60015148D1 DE 60015148 T DE60015148 T DE 60015148T DE 60015148 T DE60015148 T DE 60015148T DE 60015148 D1 DE60015148 D1 DE 60015148D1
Authority
DE
Germany
Prior art keywords
dna target
target material
methods
dna
complex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60015148T
Other languages
English (en)
Other versions
DE60015148T2 (de
Inventor
M Tereba
M Bitner
C Koller
E Smith
D Kephart
J Ekenberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/377,986 external-priority patent/US6673631B1/en
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of DE60015148D1 publication Critical patent/DE60015148D1/de
Publication of DE60015148T2 publication Critical patent/DE60015148T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE60015148T 1999-08-20 2000-08-18 Simultane isolierung und quantifizierung von dna Expired - Lifetime DE60015148T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/377,986 US6673631B1 (en) 1997-01-21 1999-08-20 Simultaneous isolation and quantitation of DNA
US377986 1999-08-20
PCT/US2000/022827 WO2001014590A2 (en) 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of dna

Publications (2)

Publication Number Publication Date
DE60015148D1 true DE60015148D1 (de) 2004-11-25
DE60015148T2 DE60015148T2 (de) 2006-02-16

Family

ID=23491269

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60015148T Expired - Lifetime DE60015148T2 (de) 1999-08-20 2000-08-18 Simultane isolierung und quantifizierung von dna

Country Status (8)

Country Link
EP (1) EP1204741B1 (de)
JP (1) JP4425513B2 (de)
CN (1) CN1370230A (de)
AT (1) ATE280225T1 (de)
AU (1) AU780319B2 (de)
CA (1) CA2379503A1 (de)
DE (1) DE60015148T2 (de)
WO (1) WO2001014590A2 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4106026B2 (ja) 2001-11-28 2008-06-25 アプレラ コーポレイション 選択的な核酸の単離方法および組成物
WO2003057910A2 (en) 2002-01-08 2003-07-17 Roche Diagnostics Gmbh Use of silica material in an amplification reaction
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
EP1621890A1 (de) * 2004-07-26 2006-02-01 bioMerieux B.V. Gerät und Methode zur Separation, Mischung und Konzentrierung magnetischer Partikel mit Flüssigkeiten und deren Verwendungen in Reinigungsmethoden
EP1690938A1 (de) * 2005-02-11 2006-08-16 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden
WO2007119779A1 (ja) 2006-04-14 2007-10-25 Nec Corporation 個体識別方法および装置
JP2009065900A (ja) * 2007-09-13 2009-04-02 Olympus Corp 標的ｒｎａの解析方法及び保存方法
JP2011504592A (ja) * 2007-11-26 2011-02-10 アトノミックス アクティーゼルスカブ 双峰性サイズ分布を有する磁気粒子を備える、統合型分離および検出カートリッジ
DE102008047790A1 (de) * 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
US20120258553A1 (en) * 2009-12-23 2012-10-11 Koninklijke Philips Electronics N.V. Analyte measurement apparatus and method
US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
CN101818143A (zh) * 2010-04-28 2010-09-01 长春市博坤生物科技有限公司 基于二氧化硅磁性复合微粒的超低量dna提取试剂盒及方法
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
JP6169489B2 (ja) 2010-07-29 2017-07-26 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 一般的な試料調製
US9725754B2 (en) 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
CN110511923A (zh) * 2012-01-25 2019-11-29 基因细胞生物系统有限公司 生物分子分离
CA2868805C (en) * 2012-03-28 2018-09-04 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for the collection and isolation of nucleic acids from biological specimens
EP2891716B1 (de) * 2012-08-28 2018-03-28 Biocube System Inc. Poröse festphase zur schnellen isolierung biologischer moleküle für eine nukleinsäureamplifizierungsreaktion aus biologischen proben und verwendung davon
CN105603056B (zh) * 2014-11-21 2020-09-25 丰益(上海)生物技术研发中心有限公司 一种植物油脂掺杂检测的方法
CN104560952A (zh) * 2014-12-29 2015-04-29 福建师范大学 一种基于磁珠法快速提取革兰氏阳性菌基因组dna的方法
WO2016183292A1 (en) 2015-05-14 2016-11-17 Longhorn Vaccines And Diagnostics, Llc Rapid methods for the extraction of nucleic acids from biological samples
WO2017044710A1 (en) * 2015-09-11 2017-03-16 Corning Incorporated Compositions and methods for nucleic acid purification from blood samples
US20190329244A1 (en) * 2017-01-04 2019-10-31 Agency For Science, Technology And Research Sieve-through vertical flow system for particle-based bioassays
CN109321634A (zh) * 2017-07-26 2019-02-12 上海之江生物科技股份有限公司 核酸均一化方法及其试剂盒和应用
WO2019035335A1 (ja) * 2017-08-18 2019-02-21 Ａｇｃ株式会社 核酸の回収方法、核酸結合用担体および核酸回収キット
US20230159912A1 (en) * 2021-11-23 2023-05-25 Bioo Scientific Corporation Compositions and methods for nucleic acid normalization

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Also Published As

Publication number Publication date
JP2003507049A (ja) 2003-02-25
JP4425513B2 (ja) 2010-03-03
EP1204741B1 (de) 2004-10-20
WO2001014590A2 (en) 2001-03-01
AU780319B2 (en) 2005-03-17
DE60015148T2 (de) 2006-02-16
CN1370230A (zh) 2002-09-18
CA2379503A1 (en) 2001-03-01
AU6789500A (en) 2001-03-19
EP1204741A2 (de) 2002-05-15
WO2001014590A3 (en) 2001-10-11
ATE280225T1 (de) 2004-11-15

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