CN1332802A - 用甲基营养酵母表达系统生产重组莫内林 - Google Patents

用甲基营养酵母表达系统生产重组莫内林 Download PDF

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Publication number
CN1332802A
CN1332802A CN99815165A CN99815165A CN1332802A CN 1332802 A CN1332802 A CN 1332802A CN 99815165 A CN99815165 A CN 99815165A CN 99815165 A CN99815165 A CN 99815165A CN 1332802 A CN1332802 A CN 1332802A
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CN
China
Prior art keywords
nucleic acid
neilin
pichia pastoris
dna
isolating nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN99815165A
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English (en)
Chinese (zh)
Inventor
段陵浔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GenWay Biotech Inc
Original Assignee
GenWay Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GenWay Biotech Inc filed Critical GenWay Biotech Inc
Publication of CN1332802A publication Critical patent/CN1332802A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN99815165A 1998-12-31 1999-12-09 用甲基营养酵母表达系统生产重组莫内林 Pending CN1332802A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11452998P 1998-12-31 1998-12-31
US60/114,529 1998-12-31

Publications (1)

Publication Number Publication Date
CN1332802A true CN1332802A (zh) 2002-01-23

Family

ID=22355787

Family Applications (1)

Application Number Title Priority Date Filing Date
CN99815165A Pending CN1332802A (zh) 1998-12-31 1999-12-09 用甲基营养酵母表达系统生产重组莫内林

Country Status (9)

Country Link
EP (1) EP1141304A2 (ko)
JP (1) JP2002534074A (ko)
KR (1) KR20020008819A (ko)
CN (1) CN1332802A (ko)
AU (1) AU3116100A (ko)
BR (1) BR9916724A (ko)
CA (1) CA2364005A1 (ko)
IL (1) IL143771A0 (ko)
WO (1) WO2000040603A2 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023222111A1 (zh) * 2022-05-20 2023-11-23 南京百斯杰生物工程有限公司 具有高甜度的甜味蛋白莫内林(Monellin)突变体及其制备方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3998798A (en) * 1972-12-22 1976-12-21 The Trustees Of The University Of Pennsylvania Monellin, a sweet polypeptide derived from fruit of dioscoreophyllum cumminsii
ATE108185T1 (de) * 1987-06-19 1994-07-15 Univ California Neue klasse von niedrigkalorischen proteinsüssstoffen.
US6001410A (en) * 1996-07-25 1999-12-14 International Flavors & Fragrances Inc. Fruit liqueur beverage containing recombinant monellin to enhance the alcoholic impact

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023222111A1 (zh) * 2022-05-20 2023-11-23 南京百斯杰生物工程有限公司 具有高甜度的甜味蛋白莫内林(Monellin)突变体及其制备方法

Also Published As

Publication number Publication date
WO2000040603A3 (en) 2000-09-21
IL143771A0 (en) 2002-04-21
CA2364005A1 (en) 2000-07-13
BR9916724A (pt) 2001-09-11
WO2000040603A2 (en) 2000-07-13
AU3116100A (en) 2000-07-24
EP1141304A2 (en) 2001-10-10
KR20020008819A (ko) 2002-01-31
JP2002534074A (ja) 2002-10-15

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