CA2364005A1 - Production of recombinant monellin using methylotrophic yeast expression system - Google Patents

Production of recombinant monellin using methylotrophic yeast expression system Download PDF

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Publication number
CA2364005A1
CA2364005A1 CA002364005A CA2364005A CA2364005A1 CA 2364005 A1 CA2364005 A1 CA 2364005A1 CA 002364005 A CA002364005 A CA 002364005A CA 2364005 A CA2364005 A CA 2364005A CA 2364005 A1 CA2364005 A1 CA 2364005A1
Authority
CA
Canada
Prior art keywords
nucleic acid
amino acid
chimeric protein
monellin
isolated nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA002364005A
Other languages
English (en)
French (fr)
Inventor
Lingxun Duan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GenWay Biotech Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2364005A1 publication Critical patent/CA2364005A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CA002364005A 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system Pending CA2364005A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11452998P 1998-12-31 1998-12-31
US60/114,529 1998-12-31
PCT/US1999/029213 WO2000040603A2 (en) 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system

Publications (1)

Publication Number Publication Date
CA2364005A1 true CA2364005A1 (en) 2000-07-13

Family

ID=22355787

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002364005A Pending CA2364005A1 (en) 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system

Country Status (9)

Country Link
EP (1) EP1141304A2 (ko)
JP (1) JP2002534074A (ko)
KR (1) KR20020008819A (ko)
CN (1) CN1332802A (ko)
AU (1) AU3116100A (ko)
BR (1) BR9916724A (ko)
CA (1) CA2364005A1 (ko)
IL (1) IL143771A0 (ko)
WO (1) WO2000040603A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117088958A (zh) * 2022-05-20 2023-11-21 南京百斯杰生物工程有限公司 具有高甜度的甜味蛋白莫内林(Monellin)突变体及其制备方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3998798A (en) * 1972-12-22 1976-12-21 The Trustees Of The University Of Pennsylvania Monellin, a sweet polypeptide derived from fruit of dioscoreophyllum cumminsii
ATE108185T1 (de) * 1987-06-19 1994-07-15 Univ California Neue klasse von niedrigkalorischen proteinsüssstoffen.
US6001410A (en) * 1996-07-25 1999-12-14 International Flavors & Fragrances Inc. Fruit liqueur beverage containing recombinant monellin to enhance the alcoholic impact

Also Published As

Publication number Publication date
EP1141304A2 (en) 2001-10-10
KR20020008819A (ko) 2002-01-31
AU3116100A (en) 2000-07-24
CN1332802A (zh) 2002-01-23
JP2002534074A (ja) 2002-10-15
WO2000040603A3 (en) 2000-09-21
BR9916724A (pt) 2001-09-11
WO2000040603A2 (en) 2000-07-13
IL143771A0 (en) 2002-04-21

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