CN1296492C - 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 - Google Patents

一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 Download PDF

Info

Publication number
CN1296492C
CN1296492C CNB2004100904233A CN200410090423A CN1296492C CN 1296492 C CN1296492 C CN 1296492C CN B2004100904233 A CNB2004100904233 A CN B2004100904233A CN 200410090423 A CN200410090423 A CN 200410090423A CN 1296492 C CN1296492 C CN 1296492C
Authority
CN
China
Prior art keywords
nucleic acid
sequence
capture probe
overhang
acid capture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB2004100904233A
Other languages
English (en)
Chinese (zh)
Other versions
CN1635165A (zh
Inventor
邵威
赵永超
孙义民
乔继英
魏华江
杨卫平
周玉祥
程京
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tsinghua University
CapitalBio Corp
Original Assignee
Tsinghua University
CapitalBio Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CNB2004100904233A priority Critical patent/CN1296492C/zh
Application filed by Tsinghua University, CapitalBio Corp filed Critical Tsinghua University
Priority to CA002586408A priority patent/CA2586408A1/en
Priority to JP2007541638A priority patent/JP4664373B2/ja
Priority to EP04797371A priority patent/EP1816476B1/en
Priority to PCT/CN2004/001340 priority patent/WO2006053463A1/zh
Priority to US11/791,157 priority patent/US8680016B2/en
Priority to AU2004324960A priority patent/AU2004324960A1/en
Priority to AT04797371T priority patent/ATE545030T1/de
Publication of CN1635165A publication Critical patent/CN1635165A/zh
Application granted granted Critical
Publication of CN1296492C publication Critical patent/CN1296492C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB2004100904233A 2004-11-18 2004-11-18 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法 Expired - Fee Related CN1296492C (zh)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CNB2004100904233A CN1296492C (zh) 2004-11-18 2004-11-18 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法
JP2007541638A JP4664373B2 (ja) 2004-11-18 2004-11-23 バイオチップをベースにした核酸結合タンパク質の試験方法
EP04797371A EP1816476B1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip
PCT/CN2004/001340 WO2006053463A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip
CA002586408A CA2586408A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip
US11/791,157 US8680016B2 (en) 2004-11-18 2004-11-23 Testing method of nucleic acid binding protein based on biochip
AU2004324960A AU2004324960A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip
AT04797371T ATE545030T1 (de) 2004-11-18 2004-11-23 Testverfahren für nukleinsäurebindendes protein auf biochipbasis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNB2004100904233A CN1296492C (zh) 2004-11-18 2004-11-18 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法

Publications (2)

Publication Number Publication Date
CN1635165A CN1635165A (zh) 2005-07-06
CN1296492C true CN1296492C (zh) 2007-01-24

Family

ID=34847597

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB2004100904233A Expired - Fee Related CN1296492C (zh) 2004-11-18 2004-11-18 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法

Country Status (8)

Country Link
US (1) US8680016B2 (https=)
EP (1) EP1816476B1 (https=)
JP (1) JP4664373B2 (https=)
CN (1) CN1296492C (https=)
AT (1) ATE545030T1 (https=)
AU (1) AU2004324960A1 (https=)
CA (1) CA2586408A1 (https=)
WO (1) WO2006053463A1 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8394590B2 (en) 2006-08-02 2013-03-12 California Institute Of Technology Capture agents and related methods and systems for detecting and/or sorting targets
CA2659745A1 (en) 2006-08-02 2008-02-07 California Institute Of Technology Methods and systems for detecting and/or sorting targets
EP2167633A4 (en) 2007-07-16 2014-12-24 California Inst Of Techn MICROFLUIDIC DEVICES, METHODS AND SYSTEMS FOR DETECTING TARGET MOLECULES
CN102112626A (zh) * 2008-04-09 2011-06-29 加州理工学院 用于检测和/或分类靶标的捕获剂以及相关方法和系统
KR101090982B1 (ko) 2009-03-16 2011-12-08 경희대학교 산학협력단 양자점-앱타머 접합체를 이용한 생분석 방법
JP2012524907A (ja) * 2009-04-24 2012-10-18 ディスカヴァーエックス コーポレイション 検出可能なタンパク質を用いた細胞分析
FI3489685T3 (fi) 2011-11-21 2023-12-21 Zoetis Services Llc Signaalin vahvistaminen lateraalivirtaus- ja vastaavissa immunomäärityksissä
CN103048463A (zh) * 2012-05-02 2013-04-17 中国科学院广州生物医药与健康研究院 一种基于不同严谨度的用于检测dna结合蛋白的微孔板核酸杂交elisa方法
EP4148118A1 (en) 2012-08-24 2023-03-15 Yale University System, device and method for high-throughput multi-plexed detection
EP3062252A4 (en) * 2013-10-22 2017-06-07 KIM, Sung-Chun Marker for generating binding information on biomolecules and nucleic acids, preparation method therefor, and method and apparatus for analyzing biomolecule by using same
TWI691716B (zh) 2014-08-13 2020-04-21 美商艾巴希斯公司 電漿特異性結合搭配物檢定中之信號放大
US10584366B2 (en) 2014-12-03 2020-03-10 IsoPlexis Corporation Analysis and screening of cell secretion profiles
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
CA2991532C (en) 2015-08-04 2024-03-05 Abaxis, Inc. Signal amplification in solution-based plasmonic specific-binding partner assays
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP3538891B1 (en) 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
CN118275398A (zh) 2017-01-30 2024-07-02 硕腾服务有限责任公司 基于溶液的等离子体振子特异性结合配偶体测定和金属纳米结构
CN114072499B (zh) * 2019-04-30 2024-08-06 Encodia公司 用于制备分析物的方法和相关试剂盒
WO2024138466A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 检测组合物及蛋白捕获检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002014550A2 (en) * 2000-08-14 2002-02-21 Transgenetics Incorporated Transcription factor target gene discovery
CN1435492A (zh) * 2003-03-05 2003-08-13 东南大学 一种非标记检测dna结合蛋白的芯片及其制备、应用方法
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
WO2004087919A1 (ja) * 2003-03-28 2004-10-14 National Institute Of Advanced Industrial Science And Technology マイクロアレイを用いたアプタマーの取得方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449603A (en) * 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
WO1993015226A1 (en) 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
US20030054378A1 (en) 1997-05-08 2003-03-20 Isao Karube Method for detecting target nucleotide sequence
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
CA2327561A1 (en) * 2000-12-27 2002-06-27 Pierre Rousseau Methods for the screening and/or for the identification of differentially active nucleic acid binding factors of nucleic acid binding elements using dna microarray technology (dpa):dna proteomic array
CA2442367A1 (en) 2001-03-30 2002-10-24 Clontech Laboratories, Inc. Method for detecting multiple dna binding protein and dna interactions in a sample, and devices, systems and kits for practicing the same
US7258974B2 (en) 2001-04-23 2007-08-21 Michael F. Chou Transcription factor network discovery methods
AU2003256814A1 (en) * 2002-07-26 2004-02-16 Alfa Wasserman, Inc. Macromolecular protection assay
AU2003295692A1 (en) * 2002-11-15 2004-06-15 Sangamo Biosciences, Inc. Methods and compositions for analysis of regulatory sequences
US20040115794A1 (en) * 2002-12-12 2004-06-17 Affymetrix, Inc. Methods for detecting transcriptional factor binding sites

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002014550A2 (en) * 2000-08-14 2002-02-21 Transgenetics Incorporated Transcription factor target gene discovery
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
CN1435492A (zh) * 2003-03-05 2003-08-13 东南大学 一种非标记检测dna结合蛋白的芯片及其制备、应用方法
WO2004087919A1 (ja) * 2003-03-28 2004-10-14 National Institute Of Advanced Industrial Science And Technology マイクロアレイを用いたアプタマーの取得方法

Also Published As

Publication number Publication date
EP1816476A4 (en) 2008-08-20
JP4664373B2 (ja) 2011-04-06
CA2586408A1 (en) 2006-05-26
ATE545030T1 (de) 2012-02-15
CN1635165A (zh) 2005-07-06
WO2006053463A1 (en) 2006-05-26
EP1816476B1 (en) 2012-02-08
JP2008520212A (ja) 2008-06-19
EP1816476A1 (en) 2007-08-08
AU2004324960A1 (en) 2006-05-26
US20090018025A1 (en) 2009-01-15
US8680016B2 (en) 2014-03-25

Similar Documents

Publication Publication Date Title
CN1296492C (zh) 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法
JP2018529314A5 (https=)
JP2008520212A5 (https=)
CN101906486B (zh) 检测多种鱼类病原的基因芯片及其检测方法
JP4268944B2 (ja) 核酸の検出あるいは定量方法
CN101705299A (zh) 牛微卫星标记组合及其应用
EP2679689B1 (en) Method for improved quantification of miRNAs
CN104988144A (zh) 检测土壤中10种常见病原微生物的基因液相芯片及其检测方法
CN1745180A (zh) 用于表达基因检测的信号扩增方法
KR20090080679A (ko) 트리클로로에틸렌 노출 여부 확인용 바이오마커 및 이를이용한 확인 방법
CN104152568B (zh) 高通量str序列核心重复数检测方法
HK1079552A1 (en) A biochip-based method for nucleic acid capable of combining specific sequence binding proteins assay
HK1079552B (en) A biochip-based method for nucleic acid capable of combining specific sequence binding proteins assay
RU2402771C2 (ru) Способ скрининга сердечно-сосудистых заболеваний и биочип для осуществления этого способа
JP2023536385A (ja) コロナウイルスを含む呼吸器ウイルスを検出する構成物および方法
KR20160091262A (ko) 휘발성 유기 화합물의 인체 노출 여부 확인용 특이적 바이오마커 및 이를 이용한 휘발성 유기 화합물의 노출 여부 확인 방법
WO2014156513A1 (ja) 変異の検出方法
KR102725028B1 (ko) 바이러스성출혈성패혈증 바이러스(vhsv)를 검출하기 위한 루프 매개 등온증폭용 프라이머 세트 및 그의 용도
CN1435492A (zh) 一种非标记检测dna结合蛋白的芯片及其制备、应用方法
KR101383653B1 (ko) 프로피온알데히드 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법
HK1104852A (en) A testing method of nucleic acid binding protein based on biochip
CN1721547A (zh) 核酸外切酶ⅲ消化包被在微孔板上的含有特殊标记的双链核酸分子检测转录因子蛋白
KR101383641B1 (ko) 부틸알데히드 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법
KR101609373B1 (ko) 저급 지방족 포화 알데하이드 류 특이적 메틸화 마커 유전자 및 이를 이용한 저급 지방족 포화 알데하이드 류 노출 여부 확인 방법
CN1733933A (zh) 核酸外切酶Ⅲ消化Label－dsDNA微阵列芯片检测转录因子蛋白

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1079552

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
C56 Change in the name or address of the patentee

Owner name: CAPITALBIO CORPORATION CO., LTD.

Free format text: FORMER NAME: CAPITALBIO CORPORATION

CP01 Change in the name or title of a patent holder

Address after: 102206 Beijing City, Changping District Life Science Park Road No. 18

Patentee after: CAPITALBIO Corp.

Patentee after: TSINGHUA University

Address before: 102206 Beijing City, Changping District Life Science Park Road No. 18

Patentee before: CAPITALBIO Corp.

Patentee before: Tsinghua University

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20070124

Termination date: 20211118

CF01 Termination of patent right due to non-payment of annual fee