CA2586408A1 - A testing method of nucleic acid binding protein based on biochip - Google Patents

A testing method of nucleic acid binding protein based on biochip Download PDF

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Publication number
CA2586408A1
CA2586408A1 CA002586408A CA2586408A CA2586408A1 CA 2586408 A1 CA2586408 A1 CA 2586408A1 CA 002586408 A CA002586408 A CA 002586408A CA 2586408 A CA2586408 A CA 2586408A CA 2586408 A1 CA2586408 A1 CA 2586408A1
Authority
CA
Canada
Prior art keywords
nucleic acid
overhang
sequence
acid capture
probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002586408A
Other languages
English (en)
French (fr)
Inventor
Wei Shao
Yongchao Zhao
Yimin Sun
Jiying Qiao
Huajiang Wei
Weiping Yang
Yuxiang Zhou
Jing Cheng
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tsinghua University
CapitalBio Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2586408A1 publication Critical patent/CA2586408A1/en
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002586408A 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip Abandoned CA2586408A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CNB2004100904233A CN1296492C (zh) 2004-11-18 2004-11-18 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法
CN200410090423.3 2004-11-18
PCT/CN2004/001340 WO2006053463A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip

Publications (1)

Publication Number Publication Date
CA2586408A1 true CA2586408A1 (en) 2006-05-26

Family

ID=34847597

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002586408A Abandoned CA2586408A1 (en) 2004-11-18 2004-11-23 A testing method of nucleic acid binding protein based on biochip

Country Status (8)

Country Link
US (1) US8680016B2 (https=)
EP (1) EP1816476B1 (https=)
JP (1) JP4664373B2 (https=)
CN (1) CN1296492C (https=)
AT (1) ATE545030T1 (https=)
AU (1) AU2004324960A1 (https=)
CA (1) CA2586408A1 (https=)
WO (1) WO2006053463A1 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8394590B2 (en) 2006-08-02 2013-03-12 California Institute Of Technology Capture agents and related methods and systems for detecting and/or sorting targets
AU2007281479A1 (en) 2006-08-02 2008-02-07 California Institute Of Technology Methods and systems for detecting and/or sorting targets
WO2009012340A2 (en) 2007-07-16 2009-01-22 California Institute Of Technology Microfluidic devices, methods and systems for detecting target molecules
JP2011518553A (ja) * 2008-04-09 2011-06-30 カリフォルニア インスティチュート オブ テクノロジー 標的を検出及び／又は分類する捕捉剤および関連する方法およびシステム
KR101090982B1 (ko) 2009-03-16 2011-12-08 경희대학교 산학협력단 양자점-앱타머 접합체를 이용한 생분석 방법
WO2010124157A1 (en) * 2009-04-24 2010-10-28 Ambit Biosciences Corporation Cellular assay employing detectable protein
PT3489685T (pt) 2011-11-21 2024-01-04 Zoetis Services Llc Amplificação de sinais em imunoensaios de fluxo lateral e relacionados
CN103048463A (zh) * 2012-05-02 2013-04-17 中国科学院广州生物医药与健康研究院 一种基于不同严谨度的用于检测dna结合蛋白的微孔板核酸杂交elisa方法
CN108267581A (zh) 2012-08-24 2018-07-10 耶鲁大学 用于高通量多重检测的系统、装置和方法
CN105960644B (zh) * 2013-10-22 2018-07-10 金圣千 用于生成生物分子和核酸的结合信息的标记物及其制备方法，利用上述标记物的生物分子分析方法及装置
TWI691716B (zh) 2014-08-13 2020-04-21 美商艾巴希斯公司 電漿特異性結合搭配物檢定中之信號放大
CN112782140A (zh) 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
EP4465021A3 (en) 2015-08-04 2025-02-26 Zoetis Services LLC Signal amplification in solution-based plasmonic specific-binding partner assays
EP3510398B1 (en) 2016-09-12 2026-02-25 Isoplexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP4036578A1 (en) 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法，以及其制造方法
WO2018140953A1 (en) * 2017-01-30 2018-08-02 Abaxis, Inc. Solution-based plasmon specific-binding partner assays and metallic nanostructures
EP3963070A4 (en) * 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
CN119731334A (zh) * 2022-12-28 2025-03-28 深圳华大生命科学研究院 检测组合物及蛋白捕获检测方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449603A (en) * 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
WO1993015226A1 (en) 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
US20030054378A1 (en) * 1997-05-08 2003-03-20 Isao Karube Method for detecting target nucleotide sequence
US20030215821A1 (en) * 1999-04-20 2003-11-20 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
EP1425579A2 (en) * 2000-08-14 2004-06-09 TransGenetics Incorporated Transcription factor target gene discovery
CA2327561A1 (en) * 2000-12-27 2002-06-27 Pierre Rousseau Methods for the screening and/or for the identification of differentially active nucleic acid binding factors of nucleic acid binding elements using dna microarray technology (dpa):dna proteomic array
US20020177218A1 (en) 2001-03-30 2002-11-28 Yu Fang Methods of detecting multiple DNA binding protein and DNA interactions in a sample, and devices, systems and kits for practicing the same
WO2002086095A2 (en) 2001-04-23 2002-10-31 Chou Michael F Transcription factor network discovery methods
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
AU2003256814A1 (en) * 2002-07-26 2004-02-16 Alfa Wasserman, Inc. Macromolecular protection assay
US20060166206A1 (en) * 2002-11-15 2006-07-27 Sangamo Biosciences, Inc. Methods and compositions for analysis of regulatory sequences
US20040115794A1 (en) * 2002-12-12 2004-06-17 Affymetrix, Inc. Methods for detecting transcriptional factor binding sites
CN1435492A (zh) * 2003-03-05 2003-08-13 东南大学 一种非标记检测dna结合蛋白的芯片及其制备、应用方法
JP3944576B2 (ja) * 2003-03-28 2007-07-11 独立行政法人産業技術総合研究所 マイクロアレイを用いたアプタマーの取得方法

Also Published As

Publication number Publication date
ATE545030T1 (de) 2012-02-15
JP4664373B2 (ja) 2011-04-06
US8680016B2 (en) 2014-03-25
AU2004324960A1 (en) 2006-05-26
CN1296492C (zh) 2007-01-24
US20090018025A1 (en) 2009-01-15
EP1816476B1 (en) 2012-02-08
CN1635165A (zh) 2005-07-06
EP1816476A1 (en) 2007-08-08
JP2008520212A (ja) 2008-06-19
WO2006053463A1 (en) 2006-05-26
EP1816476A4 (en) 2008-08-20

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Legal Events

Date Code Title Description
FZDE Discontinued