CN1250743C - 测定核酸碱基序列的方法 - Google Patents

测定核酸碱基序列的方法 Download PDF

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Publication number
CN1250743C
CN1250743C CNB018213898A CN01821389A CN1250743C CN 1250743 C CN1250743 C CN 1250743C CN B018213898 A CNB018213898 A CN B018213898A CN 01821389 A CN01821389 A CN 01821389A CN 1250743 C CN1250743 C CN 1250743C
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CN
China
Prior art keywords
primer
sequence
dna polymerase
primers
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CNB018213898A
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English (en)
Chinese (zh)
Other versions
CN1483082A (zh
Inventor
上森隆司
山下裕兄
外园成和
佐藤好美
向井博之
浅田起代藏
加藤郁之进
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Takara Bio Inc
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Takara Bio Inc
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Publication of CN1483082A publication Critical patent/CN1483082A/zh
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Publication of CN1250743C publication Critical patent/CN1250743C/zh
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB018213898A 2000-10-30 2001-10-30 测定核酸碱基序列的方法 Expired - Fee Related CN1250743C (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP331513/00 2000-10-30
JP2000331513 2000-10-30
JP331513/2000 2000-10-30

Publications (2)

Publication Number Publication Date
CN1483082A CN1483082A (zh) 2004-03-17
CN1250743C true CN1250743C (zh) 2006-04-12

Family

ID=18807845

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB018213898A Expired - Fee Related CN1250743C (zh) 2000-10-30 2001-10-30 测定核酸碱基序列的方法

Country Status (8)

Country Link
US (1) US20040203008A1 (https=)
EP (1) EP1331275A4 (https=)
JP (1) JP4284063B2 (https=)
KR (1) KR100735137B1 (https=)
CN (1) CN1250743C (https=)
AU (1) AU2001296027A1 (https=)
TW (1) TWI316964B (https=)
WO (1) WO2002036822A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040185477A1 (en) * 2003-01-10 2004-09-23 Sention Inc. Methods of detecting differences in genomic sequence representation
JP5519088B2 (ja) * 2004-09-28 2014-06-11 ソニー株式会社 核酸分子塩基配列の決定方法及び決定装置
EP4230747A3 (en) 2008-03-28 2023-11-15 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CN101638691B (zh) * 2009-08-28 2011-12-21 陆学东 用于核酸pcr产物直接测序的测序引物和测序方法
KR101183199B1 (ko) 2009-11-16 2012-09-14 (주)지노믹트리 지노타이핑 방법
KR101613612B1 (ko) 2010-07-29 2016-04-20 다카라 바이오 가부시키가이샤 표적 염기를 검출하기 위한 rna를 함유한 프로브를 제조하기 위한 방법
CA2931140C (en) * 2014-02-05 2022-08-30 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Error-free sequencing of dna
CN111429969A (zh) * 2020-03-31 2020-07-17 中国人民解放军军事科学院军事医学研究院 一种细菌中功能性前噬菌体及其位置与序列的检测方法
US11649441B2 (en) * 2020-10-07 2023-05-16 Abclonal Science, Inc. Taq DNA polymerase mutants for probe qPCR

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3379774B2 (ja) * 1992-10-16 2003-02-24 タカラバイオ株式会社 塩基配列決定方法
CA2185590A1 (en) * 1995-09-18 1997-03-19 Kazunori Okano Dna sequencing method and dna sample preparation method
US5935793A (en) * 1996-09-27 1999-08-10 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method using tagged primers
JPH10191999A (ja) * 1997-01-17 1998-07-28 Hitachi Ltd Dna塩基配列決定用試料作製方法及び試薬キット
WO1999007883A1 (en) * 1997-08-11 1999-02-18 Visible Genetics Inc. Method and kit for hla class i typing dna
GB9717061D0 (en) * 1997-08-13 1997-10-15 Tepnel Medical Ltd Amplification of nucleic acids
JPH11341986A (ja) * 1998-06-02 1999-12-14 Hitachi Ltd Dna試料調製法および試薬
AUPQ008799A0 (en) * 1999-04-30 1999-05-27 Tillett, Daniel Genome sequencing

Also Published As

Publication number Publication date
KR100735137B1 (ko) 2007-07-03
CN1483082A (zh) 2004-03-17
US20040203008A1 (en) 2004-10-14
EP1331275A4 (en) 2004-08-04
EP1331275A1 (en) 2003-07-30
JP4284063B2 (ja) 2009-06-24
WO2002036822A1 (fr) 2002-05-10
TWI316964B (https=) 2009-11-11
KR20030045124A (ko) 2003-06-09
JPWO2002036822A1 (ja) 2004-03-11
AU2001296027A1 (en) 2002-05-15

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Granted publication date: 20060412

Termination date: 20101030