CN1187451C - 在转化的酵母细胞中生产蛋白质的方法 - Google Patents

在转化的酵母细胞中生产蛋白质的方法 Download PDF

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Publication number
CN1187451C
CN1187451C CNB998087068A CN99808706A CN1187451C CN 1187451 C CN1187451 C CN 1187451C CN B998087068 A CNB998087068 A CN B998087068A CN 99808706 A CN99808706 A CN 99808706A CN 1187451 C CN1187451 C CN 1187451C
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China
Prior art keywords
yeast
plasmid
gene
dna
marker gene
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Expired - Lifetime
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CNB998087068A
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English (en)
Chinese (zh)
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CN1309712A (zh
Inventor
T·杰尔德森
K·瓦德
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Novo Nordisk AS
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Novo Nordisk AS
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245Escherichia (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CNB998087068A 1998-07-16 1999-07-02 在转化的酵母细胞中生产蛋白质的方法 Expired - Lifetime CN1187451C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA199800945 1998-07-16
DKPA199800945 1998-07-16
DKPA199900754 1999-05-28
DKPA199900754 1999-05-28

Publications (2)

Publication Number Publication Date
CN1309712A CN1309712A (zh) 2001-08-22
CN1187451C true CN1187451C (zh) 2005-02-02

Family

ID=26064588

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB998087068A Expired - Lifetime CN1187451C (zh) 1998-07-16 1999-07-02 在转化的酵母细胞中生产蛋白质的方法

Country Status (16)

Country Link
EP (1) EP1097228B1 (hu)
JP (1) JP4668414B2 (hu)
KR (1) KR20010071929A (hu)
CN (1) CN1187451C (hu)
AT (1) ATE318919T1 (hu)
AU (1) AU4499399A (hu)
BR (1) BR9912106B1 (hu)
CA (1) CA2337635A1 (hu)
DE (1) DE69930118T2 (hu)
ES (1) ES2260917T3 (hu)
HU (1) HUP0102697A3 (hu)
IL (1) IL140403A0 (hu)
NO (1) NO20010246L (hu)
PL (1) PL201350B1 (hu)
RU (1) RU2238323C2 (hu)
WO (1) WO2000004172A1 (hu)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100783287B1 (ko) * 2006-08-09 2007-12-06 한국생명공학연구원 텔로미어 전사 비활성화에 관여하는 화합물의 동정을 위한균주의 제조
JP2016521701A (ja) 2013-06-07 2016-07-25 ノヴォ ノルディスク アー/エス 成熟インスリンポリペプチドを作製するための方法
WO2020106599A1 (en) * 2018-11-19 2020-05-28 Basf Se A recombinant yeast cell

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE238425T1 (de) * 1993-07-23 2003-05-15 Dsm Nv Selektionmarker-genfreie rekombinante stämme: verfahren zur ihrer herstellung und die verwendung dieser stämme
DE4428402A1 (de) * 1994-08-11 1996-02-15 Boehringer Mannheim Gmbh Gentherapeutisches Verfahren unter Verwendung von DNA-Vektoren ohne Selektionsmarker-Gen
EP0814165B1 (en) * 1996-06-21 2003-04-09 Suntory Limited Method for constructing transformed yeast cells, that do not contain a selective marker gene
JP4090090B2 (ja) * 1996-06-21 2008-05-28 サントリー株式会社 選択マーカー遺伝子を持たない形質転換体の作製方法

Also Published As

Publication number Publication date
NO20010246D0 (no) 2001-01-15
JP4668414B2 (ja) 2011-04-13
AU4499399A (en) 2000-02-07
ATE318919T1 (de) 2006-03-15
BR9912106A (pt) 2001-05-02
EP1097228A1 (en) 2001-05-09
WO2000004172A1 (en) 2000-01-27
BR9912106B1 (pt) 2012-09-04
HUP0102697A2 (hu) 2001-11-28
KR20010071929A (ko) 2001-07-31
RU2238323C2 (ru) 2004-10-20
CN1309712A (zh) 2001-08-22
EP1097228B1 (en) 2006-03-01
JP2002520061A (ja) 2002-07-09
IL140403A0 (en) 2002-02-10
NO20010246L (no) 2001-01-15
CA2337635A1 (en) 2000-01-27
DE69930118D1 (de) 2006-04-27
PL345584A1 (en) 2001-12-17
ES2260917T3 (es) 2006-11-01
DE69930118T2 (de) 2006-11-02
HUP0102697A3 (en) 2003-09-29
PL201350B1 (pl) 2009-04-30

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