CN1184322C - 通过分批或半连续培养大量生产紫杉醇的方法 - Google Patents
通过分批或半连续培养大量生产紫杉醇的方法 Download PDFInfo
- Publication number
- CN1184322C CN1184322C CNB011448776A CN01144877A CN1184322C CN 1184322 C CN1184322 C CN 1184322C CN B011448776 A CNB011448776 A CN B011448776A CN 01144877 A CN01144877 A CN 01144877A CN 1184322 C CN1184322 C CN 1184322C
- Authority
- CN
- China
- Prior art keywords
- taxol
- culture
- syg
- taxinol
- mass production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Epoxy Compounds (AREA)
Abstract
Description
仪器 | HPLC(Waters,U.S.A.) |
柱 | Capcell Pack C18UG 120(长度:250mm,内径:4.6mm) |
柱温度 | 40℃ |
流动相 | CH3CN:水(20-100%梯度) |
流速 | 1.0毫升/分钟 |
注射体积 | 10μl |
检测器 | UV(227nm),ATTE=3 |
中国紫杉SYG-1 | 特征 |
大小 | 50-150μm |
可移动性 | (-) |
凝集性 | 弱 |
聚集性 | (+) |
剪切应力的适应性 | 强 |
培养物颜色 | 淡棕色 |
培养温度 | 24℃ |
特征 | SYG-1 | USP5407816 | USP5019504 | WO92/13961 |
紫杉醇分泌模式 | 胞内 | 胞外 | 不清楚 | 不清楚 |
光度要求 | 暗处 | 需光 | 暗处 | 暗处 |
紫杉醇产率 | ~300mg/L | 153mg/L | 1-3mg/L | 0.04%DCW |
Claims (2)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950010204A KR960037826A (ko) | 1995-04-27 | 1995-04-27 | 택서스속 식물세포의 반연속식 배양방법 |
KR10204/1995 | 1995-04-27 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN96190419A Division CN1084390C (zh) | 1995-04-27 | 1996-04-27 | 通过半连续培养大量生产紫杉醇的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1394961A CN1394961A (zh) | 2003-02-05 |
CN1184322C true CN1184322C (zh) | 2005-01-12 |
Family
ID=19413178
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN96190419A Expired - Lifetime CN1084390C (zh) | 1995-04-27 | 1996-04-27 | 通过半连续培养大量生产紫杉醇的方法 |
CNB011448776A Expired - Lifetime CN1184322C (zh) | 1995-04-27 | 1996-04-27 | 通过分批或半连续培养大量生产紫杉醇的方法 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN96190419A Expired - Lifetime CN1084390C (zh) | 1995-04-27 | 1996-04-27 | 通过半连续培养大量生产紫杉醇的方法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US5871979A (zh) |
EP (2) | EP1164197B1 (zh) |
JP (2) | JP3066873B2 (zh) |
KR (1) | KR960037826A (zh) |
CN (2) | CN1084390C (zh) |
AU (1) | AU5516696A (zh) |
DE (2) | DE69635873T2 (zh) |
WO (1) | WO1996034110A1 (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7264951B1 (en) | 1992-02-20 | 2007-09-04 | Phyton, Inc. | Enhanced production of taxol and taxanes by cell cultures of Taxus species |
US6248572B1 (en) * | 1995-04-27 | 2001-06-19 | Samyang, Genex, Corporation | Production of taxol from taxus plant cell culture adding silver nitrate |
DK0954596T3 (da) * | 1996-05-24 | 2006-05-15 | Phyton Inc | Foröget produktion af taxaner ved celledyrkning af Taxus-art |
TW200304947A (en) * | 2002-02-08 | 2003-10-16 | Bristol Myers Squibb Co | Compositions and methods for latering biosynthesis of taxanes and taxane-related compounds |
WO2006129988A1 (en) * | 2005-06-03 | 2006-12-07 | Samyang Genex Corporation | Mass production of secondary metabolite in plant cell culture by treatment of saccharide mixture in medicum |
US8568798B2 (en) | 2009-04-03 | 2013-10-29 | Dianaplantsciences, S.A.S. | Production and extraction of procyanidins from plant cell cultures |
CN101696436B (zh) * | 2009-10-30 | 2011-09-28 | 广东科伦药业有限公司 | 培养红豆杉植物细胞生产紫杉醇的专用培养基 |
CN103237457A (zh) | 2010-10-04 | 2013-08-07 | 戴安娜植物科学有限公司 | 从植物细胞培养物产生和提取原花青素 |
CN105349592B (zh) * | 2015-12-09 | 2018-11-27 | 天津艾赛博生物技术有限公司 | 代谢互补的植物细胞系混种培养生产次级代谢产物的方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3539180A1 (de) * | 1985-11-05 | 1987-05-07 | Consortium Elektrochem Ind | Aureobasidium-pullulans-stamm, herstellungsverfahren und verwendung |
US5019504A (en) * | 1989-03-23 | 1991-05-28 | The United States Of America As Represented By The Secretary Of Agriculture | Production of taxol or taxol-like compounds in cell culture |
ATE146527T1 (de) * | 1991-02-12 | 1997-01-15 | Nippon Steel Corp | Verfahren zur herstellung von taxol mittels taxusspezies-zellkultur |
US5344775A (en) * | 1991-11-15 | 1994-09-06 | Escagenetics Corporation | Synthesis of taxanes in culture using pseudocalluscells |
US5407816A (en) * | 1992-02-20 | 1995-04-18 | Phyton Catalytic, Inc. | Enhanced production of taxol and taxanes by cell cultures of taxus species |
CA2131532A1 (en) * | 1992-04-01 | 1993-10-14 | Steven R. Wann | Induction of somatic embryogenesis in taxus, and the production of taxane-ring containing alkaloids therefrom |
US5279953A (en) * | 1992-06-24 | 1994-01-18 | Esca Genetics Corporation | In vivo production of taxanes |
DE69426692T2 (de) * | 1993-11-15 | 2001-06-21 | Mitsui Chemicals Inc | Methode zur herstellung von taxan-diterpen und methode zum einten von kulturzellen, die taxan-diterpen in hohen ausbeuten herstellen |
-
1995
- 1995-04-27 KR KR1019950010204A patent/KR960037826A/ko active IP Right Grant
-
1996
- 1996-04-27 DE DE69635873T patent/DE69635873T2/de not_active Expired - Lifetime
- 1996-04-27 DE DE69623223T patent/DE69623223T2/de not_active Revoked
- 1996-04-27 CN CN96190419A patent/CN1084390C/zh not_active Expired - Lifetime
- 1996-04-27 JP JP8532385A patent/JP3066873B2/ja not_active Expired - Lifetime
- 1996-04-27 EP EP01123786A patent/EP1164197B1/en not_active Expired - Lifetime
- 1996-04-27 AU AU55166/96A patent/AU5516696A/en not_active Abandoned
- 1996-04-27 EP EP96912316A patent/EP0769064B1/en not_active Revoked
- 1996-04-27 WO PCT/KR1996/000060 patent/WO1996034110A1/en active IP Right Grant
- 1996-04-27 CN CNB011448776A patent/CN1184322C/zh not_active Expired - Lifetime
- 1996-04-27 US US08/652,492 patent/US5871979A/en not_active Expired - Lifetime
-
2000
- 2000-02-15 JP JP2000036519A patent/JP3331204B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CN1084390C (zh) | 2002-05-08 |
JP3066873B2 (ja) | 2000-07-17 |
JP3331204B2 (ja) | 2002-10-07 |
JP2000197497A (ja) | 2000-07-18 |
KR960037826A (ko) | 1996-11-19 |
CN1153533A (zh) | 1997-07-02 |
AU5516696A (en) | 1996-11-18 |
JPH10504726A (ja) | 1998-05-12 |
CN1394961A (zh) | 2003-02-05 |
EP1164197B1 (en) | 2006-03-08 |
DE69623223T2 (de) | 2003-04-30 |
EP0769064A1 (en) | 1997-04-23 |
US5871979A (en) | 1999-02-16 |
EP0769064B1 (en) | 2002-08-28 |
WO1996034110A1 (en) | 1996-10-31 |
DE69635873T2 (de) | 2006-11-23 |
DE69623223D1 (de) | 2002-10-02 |
DE69635873D1 (de) | 2006-05-04 |
EP1164197A1 (en) | 2001-12-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1082546C (zh) | 植物细胞培养期间改变培养基温度来大量生产紫杉醇的方法 | |
M. Cusidó et al. | Improved paclitaxel and baccatin III production in suspension cultures of Taxusmedia | |
Fett-Neto et al. | Cell culture of Taxus as a source of the antineoplastic drug taxol and related taxanes | |
EP0642586A4 (en) | CULTURAL -i (TAXUS) FABRIC AS A SOURCE OF TAXOL, RELATED TAXANS AND OTHER NEW ANTI-TUMOR / ANTI-VIRAL SUBSTANCES (01/20/94). | |
JP2008131955A (ja) | タクスス・シネンシスの細胞培養からタキソール及び他のタキサンの合計の収量および回収を増強させる方法 | |
CN1184322C (zh) | 通过分批或半连续培养大量生产紫杉醇的方法 | |
JP2795542B2 (ja) | イチイ種の胚培養からタキソールおよびタキサン類を生産する方法 | |
EP0568821A1 (en) | Callus cell induction and the preparation of taxanes | |
CA2080000A1 (en) | Process for producing taxol by cell culture of taxus species | |
US6248572B1 (en) | Production of taxol from taxus plant cell culture adding silver nitrate | |
US6638742B1 (en) | Methods for obtaining taxanes | |
CN1212404C (zh) | 一种提高埃博霉素a产量的发酵工艺方法 | |
Syklowska-Baranek et al. | Taxane production in suspension culture of Taxus× media var. Hicksii carried out in flasks and bioreactor | |
KR0169079B1 (ko) | 탁산형 디테르펜의 제조방법 | |
CN101054561A (zh) | 一种可将糖基水解为羟基的微生物及其应用 | |
CN1118578C (zh) | 一种利用中国红豆杉细胞生产紫杉烷类的方法 | |
JP3897267B2 (ja) | タキサン型ジテルペンの製造方法 | |
KR100428634B1 (ko) | 진균을 이용한 탁솔의 제조 | |
CN1209168A (zh) | 由植物细胞培养物生产植物化学物质的生产和筛选方法 | |
CN1156581C (zh) | 脂质体包封的浆果赤霉素iii衍生物及其用途 | |
CN117448174A (zh) | 一株红树林沉积物来源真菌及其应用 | |
Wickremesinhe | Callus and cell suspension cultures of Taxus as a source of taxol and related taxanes | |
KR20010072758A (ko) | C-4 데아세틸탁산의 제조 방법 | |
KR20000037548A (ko) | 주목나무를 이용한 택솔의 제조방법 | |
CN1046350A (zh) | 细胞分裂素混合粉剂及其制造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: SAMYANG GENEX BIO CO., LTD. Free format text: FORMER OWNER: SAMYANG GENEX CORP. Effective date: 20130516 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20130516 Address after: Seoul, South Korea Patentee after: Nicks, Ji biological Miyaki Address before: Seoul, South Kerean Patentee before: Samyang Genex Co., Ltd. |
|
ASS | Succession or assignment of patent right |
Owner name: SAMYANG BIOPHARMACEUTICALS CORPORATION Free format text: FORMER OWNER: SAMYANG GENEX BIO CO., LTD. Effective date: 20140313 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20140313 Address after: Seoul, South Kerean Patentee after: Samyang Biopharmaceuticals Corporation Address before: Seoul, South Korea Patentee before: Nicks, Ji biological Miyaki |
|
CX01 | Expiry of patent term |
Granted publication date: 20050112 |
|
EXPY | Termination of patent right or utility model |