KR960037826A - 택서스속 식물세포의 반연속식 배양방법 - Google Patents

택서스속 식물세포의 반연속식 배양방법 Download PDF

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Publication number
KR960037826A
KR960037826A KR1019950010204A KR19950010204A KR960037826A KR 960037826 A KR960037826 A KR 960037826A KR 1019950010204 A KR1019950010204 A KR 1019950010204A KR 19950010204 A KR19950010204 A KR 19950010204A KR 960037826 A KR960037826 A KR 960037826A
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South Korea
Prior art keywords
taxus
plant cells
semi
culturing
taxol
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KR1019950010204A
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English (en)
Inventor
스탈허트 로이
리 아담스 토미
최형균
김상익
윤정환
홍승서
이현수
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김경환
주식회사 삼양제넥스
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=19413178&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR960037826(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 김경환, 주식회사 삼양제넥스 filed Critical 김경환
Priority to KR1019950010204A priority Critical patent/KR960037826A/ko
Priority to DE69635873T priority patent/DE69635873T2/de
Priority to DE69623223T priority patent/DE69623223T2/de
Priority to CNB011448776A priority patent/CN1184322C/zh
Priority to EP96912316A priority patent/EP0769064B1/en
Priority to EP01123786A priority patent/EP1164197B1/en
Priority to JP8532385A priority patent/JP3066873B2/ja
Priority to AU55166/96A priority patent/AU5516696A/en
Priority to US08/652,492 priority patent/US5871979A/en
Priority to PCT/KR1996/000060 priority patent/WO1996034110A1/en
Priority to CN96190419A priority patent/CN1084390C/zh
Priority to KR1019960704669A priority patent/KR0184848B1/ko
Publication of KR960037826A publication Critical patent/KR960037826A/ko
Priority to US09/218,406 priority patent/US6248572B1/en
Priority to JP2000036519A priority patent/JP3331204B2/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Epoxy Compounds (AREA)

Abstract

본 발명은 택솔의 대량생산을 위한 택서스속 식물세포의 반연속식 배양방법에 관한 것이다. 본 발명의 반연속적 배양방법은 택서스속 식물세포의 배양도중 당을 첨가하여 거듭 배양한 다음, 배양액의 일부를 새로운 세포 배양배지로 재희석하고 다시 배양하여 택솔 생산의 새로운 사이클이 시작되도록 하고, 나머지 배양액은 다른 발효조로 옮겨 다시 당을 첨가하고 배양함으로써, 택솔 생산 식물세포를 배양하는 방법인다. 본 발명의 반연속식 배양법을 이용하여 택서스속 식물세포를 배양하면, 미생물에 의한 오염을 방지하면서 택속을 생산하는 식물세포를 고수율로 배양할 수 있다.

Description

택서스속 식물세포의 반연속식 배양방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (3)

  1. (i) 택서스속(Taxus genus) 식물세포의 배양개시와 함께 배양액에 대하여 1 내지 10％( w/v)의 당(sugar)을 첨가하여, 5 내지 30일 되는 시점까지 배양하는 공정; 및, (ii) 전기에서 배양된 총 배양액의 1/10 내지 1/2(v/v)을 다른 발효조로 옮겨 공정 (i)을 반복시키고, 나머지 배양액에는 0.5 내지 10％(w/v)의 당을 첨가하여 택솔의 생산이 최고로 되는 시간까지 배양하는 공정을 포함하는 택서스속 식물세포의 반연속적 배양방법.
  2. 제1항에 있어서, 택서스속(Taxusgenus) 식물세포는 택서스 브레비폴리아(Taxus brevifolia), 택서스 카나덴시스(Taxus canadensis), 택서스 쿠스피다타(Taxus cuspidata), 택서스 바카타(Taxus baccata), 택서스 글로보사(Taxus globosa), 택서스 플로리다나(Taxus floridana), 택서스 월리치아나(Taxus wallichiana), 택서스 메디아(Taxus media) 및 택서스 치넨시스(Taxus chinensis)로 구성된 그룹으로부터 선택되는 것을 특징으로 하는 방법.
  3. 제1항에 있어서, 택솔의 생산이 최고로 되는 시간은 배양개시 후 30 내지 60일 경과한 시간인 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019950010204A 1995-04-27 1995-04-27 택서스속 식물세포의 반연속식 배양방법 KR960037826A (ko)

Priority Applications (14)

Application Number Priority Date Filing Date Title
KR1019950010204A KR960037826A (ko) 1995-04-27 1995-04-27 택서스속 식물세포의 반연속식 배양방법
CN96190419A CN1084390C (zh) 1995-04-27 1996-04-27 通过半连续培养大量生产紫杉醇的方法
JP8532385A JP3066873B2 (ja) 1995-04-27 1996-04-27 半連続培養によるタキソールの大量生産方法
US08/652,492 US5871979A (en) 1995-04-27 1996-04-27 Method for mass production of taxol by semi-continuous culture with Taxus chinensis cell culture
CNB011448776A CN1184322C (zh) 1995-04-27 1996-04-27 通过分批或半连续培养大量生产紫杉醇的方法
EP96912316A EP0769064B1 (en) 1995-04-27 1996-04-27 A method for mass production of taxol by semi-continuous culture
EP01123786A EP1164197B1 (en) 1995-04-27 1996-04-27 A method for mass production of taxol by elicitations
DE69635873T DE69635873T2 (de) 1995-04-27 1996-04-27 Verfahren zur Massenproduktion von Taxol mittels Induktion
AU55166/96A AU5516696A (en) 1995-04-27 1996-04-27 A method for mass production of taxol by semi-continuous cul ture
DE69623223T DE69623223T2 (de) 1995-04-27 1996-04-27 Methode zur massenproduktion von taxol mittels semi-kontinuierlicher kultur
PCT/KR1996/000060 WO1996034110A1 (en) 1995-04-27 1996-04-27 A method for mass production of taxol by semi-continuous culture
KR1019960704669A KR0184848B1 (en) 1995-04-27 1996-08-26 Method for mass production of taxol by semi-continuous culture
US09/218,406 US6248572B1 (en) 1995-04-27 1998-12-22 Production of taxol from taxus plant cell culture adding silver nitrate
JP2000036519A JP3331204B2 (ja) 1995-04-27 2000-02-15 半連続培養によるタキソールの大量生産方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950010204A KR960037826A (ko) 1995-04-27 1995-04-27 택서스속 식물세포의 반연속식 배양방법

Publications (1)

Publication Number Publication Date
KR960037826A true KR960037826A (ko) 1996-11-19

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ID=19413178

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950010204A KR960037826A (ko) 1995-04-27 1995-04-27 택서스속 식물세포의 반연속식 배양방법

Country Status (8)

Country Link
US (1) US5871979A (ko)
EP (2) EP1164197B1 (ko)
JP (2) JP3066873B2 (ko)
KR (1) KR960037826A (ko)
CN (2) CN1084390C (ko)
AU (1) AU5516696A (ko)
DE (2) DE69635873T2 (ko)
WO (1) WO1996034110A1 (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7264951B1 (en) 1992-02-20 2007-09-04 Phyton, Inc. Enhanced production of taxol and taxanes by cell cultures of Taxus species
US6248572B1 (en) * 1995-04-27 2001-06-19 Samyang, Genex, Corporation Production of taxol from taxus plant cell culture adding silver nitrate
HUP9901788A3 (en) * 1996-05-24 2002-07-29 Phyton Inc Ithaca Enhanced production of taxanes by cell cultures of taxus species
TW200304947A (en) * 2002-02-08 2003-10-16 Bristol Myers Squibb Co Compositions and methods for latering biosynthesis of taxanes and taxane-related compounds
WO2006129988A1 (en) * 2005-06-03 2006-12-07 Samyang Genex Corporation Mass production of secondary metabolite in plant cell culture by treatment of saccharide mixture in medicum
KR20120059451A (ko) 2009-04-03 2012-06-08 다이아나플랜트사이언스, 아이엔씨. 식물 세포 배양 유래 프로시아니딘의 생산 및 추출
CN101696436B (zh) * 2009-10-30 2011-09-28 广东科伦药业有限公司 培养红豆杉植物细胞生产紫杉醇的专用培养基
BR112013008175A2 (pt) 2010-10-04 2016-06-21 Dianaplantsciences Sas produção e extração de procianidinas de culturas celulares vegetais
CN105349592B (zh) * 2015-12-09 2018-11-27 天津艾赛博生物技术有限公司 代谢互补的植物细胞系混种培养生产次级代谢产物的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3539180A1 (de) * 1985-11-05 1987-05-07 Consortium Elektrochem Ind Aureobasidium-pullulans-stamm, herstellungsverfahren und verwendung
US5019504A (en) * 1989-03-23 1991-05-28 The United States Of America As Represented By The Secretary Of Agriculture Production of taxol or taxol-like compounds in cell culture
DE69216003D1 (de) * 1991-02-12 1997-01-30 Nippon Steel Corp Verfahren zur herstellung von taxol mittels taxusspezies-zellkultur
US5344775A (en) * 1991-11-15 1994-09-06 Escagenetics Corporation Synthesis of taxanes in culture using pseudocalluscells
US5407816A (en) * 1992-02-20 1995-04-18 Phyton Catalytic, Inc. Enhanced production of taxol and taxanes by cell cultures of taxus species
CA2131532A1 (en) * 1992-04-01 1993-10-14 Steven R. Wann Induction of somatic embryogenesis in taxus, and the production of taxane-ring containing alkaloids therefrom
US5279953A (en) * 1992-06-24 1994-01-18 Esca Genetics Corporation In vivo production of taxanes
DE69433120T2 (de) * 1993-11-15 2004-07-01 Mitsui Chemicals, Inc. Methode zur Herstellung von Taxan-Typ Diterpen und Methode zur Gewinnung von Kulturzellen die Taxan-Typ Diterpen in hohen Ausbeuten herstellen

Also Published As

Publication number Publication date
CN1184322C (zh) 2005-01-12
US5871979A (en) 1999-02-16
CN1153533A (zh) 1997-07-02
CN1084390C (zh) 2002-05-08
WO1996034110A1 (en) 1996-10-31
DE69623223D1 (de) 2002-10-02
DE69635873T2 (de) 2006-11-23
EP1164197B1 (en) 2006-03-08
CN1394961A (zh) 2003-02-05
JPH10504726A (ja) 1998-05-12
DE69635873D1 (de) 2006-05-04
EP0769064B1 (en) 2002-08-28
AU5516696A (en) 1996-11-18
EP0769064A1 (en) 1997-04-23
JP2000197497A (ja) 2000-07-18
DE69623223T2 (de) 2003-04-30
JP3331204B2 (ja) 2002-10-07
EP1164197A1 (en) 2001-12-19
JP3066873B2 (ja) 2000-07-17

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