CN117916378A - 用于制造cdp-胆碱的重组微生物和使用该重组微生物的cdp-胆碱的制造方法 - Google Patents

用于制造cdp-胆碱的重组微生物和使用该重组微生物的cdp-胆碱的制造方法 Download PDF

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Publication number
CN117916378A
CN117916378A CN202280060889.5A CN202280060889A CN117916378A CN 117916378 A CN117916378 A CN 117916378A CN 202280060889 A CN202280060889 A CN 202280060889A CN 117916378 A CN117916378 A CN 117916378A
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China
Prior art keywords
choline
ctp
amino acid
dna
acid sequence
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Pending
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CN202280060889.5A
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English (en)
Chinese (zh)
Inventor
堀一将
森田敏彦
西野恒代
氏原哲朗
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Kyowa Hakko Bio Co Ltd
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Kyowa Hakko Bio Co Ltd
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Publication of CN117916378A publication Critical patent/CN117916378A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07015—Choline-phosphate cytidylyltransferase (2.7.7.15)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202280060889.5A 2021-09-10 2022-09-09 用于制造cdp-胆碱的重组微生物和使用该重组微生物的cdp-胆碱的制造方法 Pending CN117916378A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-147985 2021-09-10
JP2021147985 2021-09-10
PCT/JP2022/033972 WO2023038128A1 (ja) 2021-09-10 2022-09-09 Ｃｄｐ－コリンの製造に用いる組換え微生物及び該組換え微生物を用いるｃｄｐ－コリンの製造方法

Publications (1)

Publication Number Publication Date
CN117916378A true CN117916378A (zh) 2024-04-19

Family

ID=85506465

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280060889.5A Pending CN117916378A (zh) 2021-09-10 2022-09-09 用于制造cdp-胆碱的重组微生物和使用该重组微生物的cdp-胆碱的制造方法

Country Status (8)

Country Link
US (1) US20250043255A1 (https=)
EP (1) EP4400588A4 (https=)
JP (1) JPWO2023038128A1 (https=)
KR (1) KR20240043794A (https=)
CN (1) CN117916378A (https=)
AU (1) AU2022344719B2 (https=)
CA (1) CA3231307A1 (https=)
WO (1) WO2023038128A1 (https=)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58110600A (ja) 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
JP2545078B2 (ja) 1987-04-06 1996-10-16 協和醗酵工業株式会社 核酸関連物質の製造法
JP3369236B2 (ja) 1992-01-30 2003-01-20 協和醗酵工業株式会社 シチジンジリン酸コリンの製造法
KR100555161B1 (ko) 1996-09-17 2006-11-30 교와 핫꼬 고교 가부시끼가이샤 당뉴클레오티드류및복합당질의제조법
US20050164359A1 (en) 2002-05-08 2005-07-28 Hashimoto Shin-Ichi Process for producing cytidine 5'-diphosphate choline
KR20070023830A (ko) 2005-08-23 2007-03-02 주식회사 엘티아이 발광다이오드를 광원으로 사용하는 백라이트 유닛 및 그의결선 방법
JP5112869B2 (ja) 2005-08-23 2013-01-09 協和発酵バイオ株式会社 シチジンジリン酸コリンの製造法
CN104774799B (zh) * 2015-04-17 2018-01-30 南京工业大学 一株表达胆碱激酶及磷酰胆碱胞苷转移酶的基因工程菌及其构建方法与应用
CN109207415B (zh) * 2017-07-07 2022-09-16 苏州华赛生物工程技术有限公司 一种生产胞二磷胆碱的重组微生物及生产胞二磷胆碱的方法
JP7401368B2 (ja) 2020-03-24 2023-12-19 フルテック株式会社 自動ドア

Also Published As

Publication number Publication date
KR20240043794A (ko) 2024-04-03
WO2023038128A1 (ja) 2023-03-16
CA3231307A1 (en) 2023-03-16
EP4400588A4 (en) 2025-10-08
US20250043255A1 (en) 2025-02-06
AU2022344719A1 (en) 2024-03-28
EP4400588A1 (en) 2024-07-17
AU2022344719B2 (en) 2025-12-11
JPWO2023038128A1 (https=) 2023-03-16

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