AU2022344719B2 - Recombinant microorganism used for producing cdp-choline, and method for producing cdp-choline using said recombinant microorganism - Google Patents

Recombinant microorganism used for producing cdp-choline, and method for producing cdp-choline using said recombinant microorganism

Info

Publication number
AU2022344719B2
AU2022344719B2 AU2022344719A AU2022344719A AU2022344719B2 AU 2022344719 B2 AU2022344719 B2 AU 2022344719B2 AU 2022344719 A AU2022344719 A AU 2022344719A AU 2022344719 A AU2022344719 A AU 2022344719A AU 2022344719 B2 AU2022344719 B2 AU 2022344719B2
Authority
AU
Australia
Prior art keywords
choline
dna
ctp
cdp
phosphocholine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2022344719A
Other languages
English (en)
Other versions
AU2022344719A1 (en
Inventor
Kazumasa Hori
Toshihiko Morita
Tsuneyo Nishino
Tetsuro Ujihara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyowa Hakko Bio Co Ltd
Original Assignee
Kyowa Hakko Bio Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Bio Co Ltd filed Critical Kyowa Hakko Bio Co Ltd
Publication of AU2022344719A1 publication Critical patent/AU2022344719A1/en
Application granted granted Critical
Publication of AU2022344719B2 publication Critical patent/AU2022344719B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07015—Choline-phosphate cytidylyltransferase (2.7.7.15)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2022344719A 2021-09-10 2022-09-09 Recombinant microorganism used for producing cdp-choline, and method for producing cdp-choline using said recombinant microorganism Active AU2022344719B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-147985 2021-09-10
JP2021147985 2021-09-10
PCT/JP2022/033972 WO2023038128A1 (ja) 2021-09-10 2022-09-09 Ｃｄｐ－コリンの製造に用いる組換え微生物及び該組換え微生物を用いるｃｄｐ－コリンの製造方法

Publications (2)

Publication Number Publication Date
AU2022344719A1 AU2022344719A1 (en) 2024-03-28
AU2022344719B2 true AU2022344719B2 (en) 2025-12-11

Family

ID=85506465

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2022344719A Active AU2022344719B2 (en) 2021-09-10 2022-09-09 Recombinant microorganism used for producing cdp-choline, and method for producing cdp-choline using said recombinant microorganism

Country Status (8)

Country Link
US (1) US20250043255A1 (https=)
EP (1) EP4400588A4 (https=)
JP (1) JPWO2023038128A1 (https=)
KR (1) KR20240043794A (https=)
CN (1) CN117916378A (https=)
AU (1) AU2022344719B2 (https=)
CA (1) CA3231307A1 (https=)
WO (1) WO2023038128A1 (https=)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58110600A (ja) 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
JP2545078B2 (ja) 1987-04-06 1996-10-16 協和醗酵工業株式会社 核酸関連物質の製造法
JP3369236B2 (ja) 1992-01-30 2003-01-20 協和醗酵工業株式会社 シチジンジリン酸コリンの製造法
KR100555161B1 (ko) 1996-09-17 2006-11-30 교와 핫꼬 고교 가부시끼가이샤 당뉴클레오티드류및복합당질의제조법
US20050164359A1 (en) 2002-05-08 2005-07-28 Hashimoto Shin-Ichi Process for producing cytidine 5'-diphosphate choline
KR20070023830A (ko) 2005-08-23 2007-03-02 주식회사 엘티아이 발광다이오드를 광원으로 사용하는 백라이트 유닛 및 그의결선 방법
JP5112869B2 (ja) 2005-08-23 2013-01-09 協和発酵バイオ株式会社 シチジンジリン酸コリンの製造法
CN104774799B (zh) * 2015-04-17 2018-01-30 南京工业大学 一株表达胆碱激酶及磷酰胆碱胞苷转移酶的基因工程菌及其构建方法与应用
CN109207415B (zh) * 2017-07-07 2022-09-16 苏州华赛生物工程技术有限公司 一种生产胞二磷胆碱的重组微生物及生产胞二磷胆碱的方法
JP7401368B2 (ja) 2020-03-24 2023-12-19 フルテック株式会社 自動ドア

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Database Uniprot 13 November 2019 uploaded URL: https://rest.uniprot.org/uniprotkb/A0A5C8H8H0.txt>. Definition: Full=Phosphocholine cytidylyltransferase family protein. Accession No.A0A5C8H8H0 *
Database Uniprot 18 May 2010 uploaded URL: https://rest.uniprot.org/uniprotkb/D4J4S5.txt Definition: SubName: Full=CTP:phosphocholine cytidylyltransferase involved in choline phosphorylation for cell surface LPS epitopes Accession No.D4J4S5 *
Database Uniprot 20 January 2009 uploaded. URL: https://rest.uniprot.org/uniprotkb/B6W AFl.txt Definition: SubName: Full=Cholinephosphate cytidylyltransferase/choline kinase. Accession No.B6WAF1 *
Database Uniprot 22 November 2017 uploaded: https://rest.uniprot.org/uniprotkb/A0A1H9I468.txt>. Definition: SubName: Full=CTP:phosphocholine cytidylyltransferase. Accession No.A0A1H9I468 *

Also Published As

Publication number Publication date
KR20240043794A (ko) 2024-04-03
WO2023038128A1 (ja) 2023-03-16
CA3231307A1 (en) 2023-03-16
EP4400588A4 (en) 2025-10-08
US20250043255A1 (en) 2025-02-06
AU2022344719A1 (en) 2024-03-28
CN117916378A (zh) 2024-04-19
EP4400588A1 (en) 2024-07-17
JPWO2023038128A1 (https=) 2023-03-16

Similar Documents

Publication Publication Date Title
JP7244613B2 (ja) 希少糖の製造法
CN110494553B (zh) 茶氨酸的制造方法
WO2022168992A1 (ja) １，３－フコシルトランスフェラーゼ活性を有する蛋白質及びフコース含有糖質の製造法
JP7763170B2 (ja) ジペプチドの製造法
AU2022344719B2 (en) Recombinant microorganism used for producing cdp-choline, and method for producing cdp-choline using said recombinant microorganism
JP7770553B2 (ja) Ｎａｍｐｔ活性を有する蛋白質およびｎｍｎの製造方法
JP6441806B2 (ja) Ｎ−アセチルノイラミン酸及びｎ−アセチルノイラミン酸含有糖質の製造法
US20260002181A1 (en) Method for producing 4-(aminomethyl)cyclohexane-1-carboxylic acid
RU2853140C2 (ru) Рекомбинантный микроорганизм, используемый для продукции цдф-холина, и способ продукции цдф-холина с использованием указанного рекомбинантного микроорганизма
JP7697887B2 (ja) 改変されたラクトースパーミアーゼを有する微生物及びラクトース含有オリゴ糖の製造法
AU2022360817A1 (en) Microorganism having ability to produce n-acetylneuraminic acid and/or n-acetylneuraminic acid-containing carbohydrate and method for producing n-acetylneuraminic acid and/or n-acetylneuraminic acid-containing carbohydrate using said microorganism
JP2003274948A (ja) Ｕｄｐ−グルクロン酸の製造法
EP4296360A1 (en) Modified protein having alpha1,2-fucosyltransferase activity and method for producing fucose-containing carbohydrate
JP2021191241A (ja) β−ポリリンゴ酸の製造法
JP2022001556A (ja) 蛋白質及び３−ヒドロキシイソ吉草酸の製造方法

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)