CN117795089A

CN117795089A - 用于改善的乙醇发酵的工程化微生物

用于改善的乙醇发酵的工程化微生物 Download PDF

Info

Publication number: CN117795089A
Authority: CN; China
Prior art keywords: seq; host cell; cell; nos; polynucleotide encoding
Prior art date: 2021-06-07
Legal status : Pending

Application number

CN202280054851.7A

Other languages

English (en)

Chinese (zh)

Inventor

E·阿兰

H·R·亚兹迪

M·G·卡特利特

C·L·斯特拉勒

Current Assignee

Novozymes AS

Original Assignee

Novozymes AS

Priority date

2021-06-07

Filing date

2022-06-06

Publication date

2024-03-29

2022-06-06 Application filed by Novozymes AS filed Critical Novozymes AS

2024-03-29 Publication of CN117795089A publication Critical patent/CN117795089A/zh

Status Pending legal-status Critical Current

Links

LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 140
238000000855 fermentation Methods 0.000 title claims abstract description 105
230000004151 fermentation Effects 0.000 title claims abstract description 104
244000005700 microbiome Species 0.000 title description 25
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 432
238000000034 method Methods 0.000 claims abstract description 132
102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims abstract description 90
108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims abstract description 90
239000000463 material Substances 0.000 claims abstract description 55
108010035824 Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) Proteins 0.000 claims abstract description 37
229920002472 Starch Polymers 0.000 claims abstract description 34
239000008107 starch Substances 0.000 claims abstract description 34
235000019698 starch Nutrition 0.000 claims abstract description 34
229920002678 cellulose Polymers 0.000 claims abstract description 31
239000001913 cellulose Substances 0.000 claims abstract description 31
XJLXINKUBYWONI-NNYOXOHSSA-O NADP(+) Chemical class NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-O 0.000 claims abstract 9
210000004027 cell Anatomy 0.000 claims description 281
102000040430 polynucleotide Human genes 0.000 claims description 193
108091033319 polynucleotide Proteins 0.000 claims description 193
239000002157 polynucleotide Substances 0.000 claims description 193
102100022624 Glucoamylase Human genes 0.000 claims description 175
108090000637 alpha-Amylases Proteins 0.000 claims description 173
102000004139 alpha-Amylases Human genes 0.000 claims description 166
108090000765 processed proteins & peptides Proteins 0.000 claims description 156
102000004196 processed proteins & peptides Human genes 0.000 claims description 155
229920001184 polypeptide Polymers 0.000 claims description 154
229940024171 alpha-amylase Drugs 0.000 claims description 145
108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 142
240000004808 Saccharomyces cerevisiae Species 0.000 claims description 130
235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 130
108091005804 Peptidases Proteins 0.000 claims description 129
150000001413 amino acids Chemical class 0.000 claims description 124
239000004365 Protease Substances 0.000 claims description 114
125000003275 alpha amino acid group Chemical group 0.000 claims description 79
108090000623 proteins and genes Proteins 0.000 claims description 73
239000000203 mixture Substances 0.000 claims description 43
238000004519 manufacturing process Methods 0.000 claims description 39
230000037361 pathway Effects 0.000 claims description 22
150000002972 pentoses Chemical class 0.000 claims description 14
210000005253 yeast cell Anatomy 0.000 claims description 13
238000003501 co-culture Methods 0.000 claims description 12
241000235070 Saccharomyces Species 0.000 claims description 11
241000235648 Pichia Species 0.000 claims description 9
241000223252 Rhodotorula Species 0.000 claims description 9
239000003795 chemical substances by application Substances 0.000 claims description 6
239000003995 emulsifying agent Substances 0.000 claims description 6
241000222120 Candida <Saccharomycetales> Species 0.000 claims description 5
108010041921 Glycerolphosphate Dehydrogenase Proteins 0.000 claims description 5
102000000587 Glycerolphosphate Dehydrogenase Human genes 0.000 claims description 5
241000235649 Kluyveromyces Species 0.000 claims description 5
241000235346 Schizosaccharomyces Species 0.000 claims description 5
241000235013 Yarrowia Species 0.000 claims description 5
239000003963 antioxidant agent Substances 0.000 claims description 5
108010032776 glycerol-1-phosphatase Proteins 0.000 claims description 5
238000012216 screening Methods 0.000 claims description 5
239000004094 surface-active agent Substances 0.000 claims description 5
230000008961 swelling Effects 0.000 claims description 5
241000722885 Brettanomyces Species 0.000 claims description 4
241001337994 Cryptococcus <scale insect> Species 0.000 claims description 4
238000012258 culturing Methods 0.000 claims description 4
241000795633 Olea <sea slug> Species 0.000 claims description 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
108010078791 Carrier Proteins Proteins 0.000 abstract description 117
102000042092 Glucose transporter family Human genes 0.000 abstract description 86
108091052347 Glucose transporter family Proteins 0.000 abstract description 86
235000001014 amino acid Nutrition 0.000 description 133
102000035195 Peptidases Human genes 0.000 description 129
229940024606 amino acid Drugs 0.000 description 114
230000000694 effects Effects 0.000 description 101
108091026890 Coding region Proteins 0.000 description 95
235000019419 proteases Nutrition 0.000 description 91
108010064785 Phospholipases Proteins 0.000 description 67
102000015439 Phospholipases Human genes 0.000 description 67
102000004190 Enzymes Human genes 0.000 description 65
108090000790 Enzymes Proteins 0.000 description 65
229940088598 enzyme Drugs 0.000 description 61
108010087472 Trehalase Proteins 0.000 description 60
102100029677 Trehalase Human genes 0.000 description 59
ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 51
238000006467 substitution reaction Methods 0.000 description 50
108010006035 Metalloproteases Proteins 0.000 description 43
102000005741 Metalloproteases Human genes 0.000 description 43
230000014509 gene expression Effects 0.000 description 43
108020004414 DNA Proteins 0.000 description 42
101100316936 African swine fever virus (strain Badajoz 1971 Vero-adapted) Ba71V-104 gene Proteins 0.000 description 35
108050008938 Glucoamylases Proteins 0.000 description 34
XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical class NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 33
238000012217 deletion Methods 0.000 description 33
230000037430 deletion Effects 0.000 description 33
241000894007 species Species 0.000 description 33
239000013598 vector Substances 0.000 description 31
125000000539 amino acid group Chemical group 0.000 description 30
240000008042 Zea mays Species 0.000 description 29
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 29
235000002017 Zea mays subsp mays Nutrition 0.000 description 29
235000005822 corn Nutrition 0.000 description 29
235000010980 cellulose Nutrition 0.000 description 28
125000003729 nucleotide group Chemical group 0.000 description 27
239000012634 fragment Substances 0.000 description 25
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 24
241000193385 Geobacillus stearothermophilus Species 0.000 description 24
102220198290 rs1057519956 Human genes 0.000 description 24
102000004169 proteins and genes Human genes 0.000 description 23
241000351920 Aspergillus nidulans Species 0.000 description 22
230000001580 bacterial effect Effects 0.000 description 22
108010076504 Protein Sorting Signals Proteins 0.000 description 21
SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 21
230000004927 fusion Effects 0.000 description 21
238000003780 insertion Methods 0.000 description 21
230000037431 insertion Effects 0.000 description 21
235000018102 proteins Nutrition 0.000 description 21
230000003197 catalytic effect Effects 0.000 description 20
230000001461 cytolytic effect Effects 0.000 description 20
SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 19
102220466851 HLA class II histocompatibility antigen, DR beta 4 chain_V59A_mutation Human genes 0.000 description 19
239000002773 nucleotide Substances 0.000 description 19
241001494489 Thielavia Species 0.000 description 18
239000002609 medium Substances 0.000 description 18
239000000047 product Substances 0.000 description 17
102220080264 rs372250472 Human genes 0.000 description 17
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
241000193830 Bacillus <bacterium> Species 0.000 description 16
PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 16
239000002361 compost Substances 0.000 description 16
239000002689 soil Substances 0.000 description 16
239000013604 expression vector Substances 0.000 description 15
241000985513 Penicillium oxalicum Species 0.000 description 14
238000003556 assay Methods 0.000 description 14
108010047754 beta-Glucosidase Proteins 0.000 description 14
102000006995 beta-Glucosidase Human genes 0.000 description 14
KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 14
108010053754 Aldehyde reductase Proteins 0.000 description 13
241000235645 Pichia kudriavzevii Species 0.000 description 13
230000002538 fungal effect Effects 0.000 description 13
150000007523 nucleic acids Chemical group 0.000 description 13
239000013612 plasmid Substances 0.000 description 13
239000000523 sample Substances 0.000 description 13
239000000758 substrate Substances 0.000 description 13
102000016912 Aldehyde Reductase Human genes 0.000 description 12
108010058076 D-xylulose reductase Proteins 0.000 description 12
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 12
108020004711 Nucleic Acid Probes Proteins 0.000 description 12
238000006460 hydrolysis reaction Methods 0.000 description 12
239000002853 nucleic acid probe Substances 0.000 description 12
102000039446 nucleic acids Human genes 0.000 description 12
108020004707 nucleic acids Proteins 0.000 description 12
UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 11
241000223218 Fusarium Species 0.000 description 11
102100026974 Sorbitol dehydrogenase Human genes 0.000 description 11
238000006243 chemical reaction Methods 0.000 description 11
230000007062 hydrolysis Effects 0.000 description 11
229920001282 polysaccharide Polymers 0.000 description 11
239000005017 polysaccharide Substances 0.000 description 11
150000004804 polysaccharides Chemical class 0.000 description 11
102220093346 rs876661018 Human genes 0.000 description 11
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 10
OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 10
108020004705 Codon Proteins 0.000 description 10
241000196324 Embryophyta Species 0.000 description 10
102100029089 Xylulose kinase Human genes 0.000 description 10
230000010076 replication Effects 0.000 description 10
108091022915 xylulokinase Proteins 0.000 description 10
240000006439 Aspergillus oryzae Species 0.000 description 9
235000002247 Aspergillus oryzae Nutrition 0.000 description 9
108010059892 Cellulase Proteins 0.000 description 9
241000123346 Chrysosporium Species 0.000 description 9
241000235403 Rhizomucor miehei Species 0.000 description 9
108700040099 Xylose isomerases Proteins 0.000 description 9
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 9
238000010367 cloning Methods 0.000 description 9
230000000295 complement effect Effects 0.000 description 9
238000013461 design Methods 0.000 description 9
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 9
235000019833 protease Nutrition 0.000 description 9
108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 8
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 8
102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 8
102000012479 Serine Proteases Human genes 0.000 description 8
108010022999 Serine Proteases Proteins 0.000 description 8
238000002105 Southern blotting Methods 0.000 description 8
PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 8
CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 8
-1 at least 25% less Chemical compound 0.000 description 8
239000012876 carrier material Substances 0.000 description 8
238000009396 hybridization Methods 0.000 description 8
HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 8
238000005457 optimization Methods 0.000 description 8
102200086056 rs41494349 Human genes 0.000 description 8
235000000346 sugar Nutrition 0.000 description 8
241000228212 Aspergillus Species 0.000 description 7
102220546833 Nuclear pore complex protein Nup85_A27K_mutation Human genes 0.000 description 7
230000010354 integration Effects 0.000 description 7
230000035772 mutation Effects 0.000 description 7
239000001384 succinic acid Substances 0.000 description 7
238000013518 transcription Methods 0.000 description 7
230000035897 transcription Effects 0.000 description 7
239000004382 Amylase Substances 0.000 description 6
101000757144 Aspergillus niger Glucoamylase Proteins 0.000 description 6
241000972773 Aulopiformes Species 0.000 description 6
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 6
125000003412 L-alanyl group Chemical group [H]N([H])[C@@](C([H])([H])[H])(C(=O)[*])[H] 0.000 description 6
108010080643 L-xylulose reductase Proteins 0.000 description 6
102100029137 L-xylulose reductase Human genes 0.000 description 6
241000228178 Thermoascus Species 0.000 description 6
230000007423 decrease Effects 0.000 description 6
239000008103 glucose Substances 0.000 description 6
108020004999 messenger RNA Proteins 0.000 description 6
230000008569 process Effects 0.000 description 6
102200081484 rs1553259760 Human genes 0.000 description 6
235000019515 salmon Nutrition 0.000 description 6
102000040811 transporter activity Human genes 0.000 description 6
108091092194 transporter activity Proteins 0.000 description 6
108010021809 Alcohol dehydrogenase Proteins 0.000 description 5
102000007698 Alcohol dehydrogenase Human genes 0.000 description 5
241000223600 Alternaria Species 0.000 description 5
241000235349 Ascomycota Species 0.000 description 5
241001225321 Aspergillus fumigatus Species 0.000 description 5
241000228245 Aspergillus niger Species 0.000 description 5
241000193744 Bacillus amyloliquefaciens Species 0.000 description 5
108010029675 Bacillus licheniformis alpha-amylase Proteins 0.000 description 5
244000063299 Bacillus subtilis Species 0.000 description 5
235000014469 Bacillus subtilis Nutrition 0.000 description 5
102000016938 Catalase Human genes 0.000 description 5
108010053835 Catalase Proteins 0.000 description 5
241000193403 Clostridium Species 0.000 description 5
ZAQJHHRNXZUBTE-WUJLRWPWSA-N D-xylulose Chemical compound OC[C@@H](O)[C@H](O)C(=O)CO ZAQJHHRNXZUBTE-WUJLRWPWSA-N 0.000 description 5
101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 5
241000221779 Fusarium sambucinum Species 0.000 description 5
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 5
102000005744 Glycoside Hydrolases Human genes 0.000 description 5
108010031186 Glycoside Hydrolases Proteins 0.000 description 5
229920002488 Hemicellulose Polymers 0.000 description 5
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 5
HEBKCHPVOIAQTA-IMJSIDKUSA-N L-arabinitol Chemical compound OC[C@H](O)C(O)[C@@H](O)CO HEBKCHPVOIAQTA-IMJSIDKUSA-N 0.000 description 5
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 5
108091000080 Phosphotransferase Proteins 0.000 description 5
241000235402 Rhizomucor Species 0.000 description 5
241000228341 Talaromyces Species 0.000 description 5
229940091771 aspergillus fumigatus Drugs 0.000 description 5
230000008859 change Effects 0.000 description 5
239000002299 complementary DNA Substances 0.000 description 5
230000002708 enhancing effect Effects 0.000 description 5
108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 description 5
108010002430 hemicellulase Proteins 0.000 description 5
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 5
102000020233 phosphotransferase Human genes 0.000 description 5
230000008488 polyadenylation Effects 0.000 description 5
238000003752 polymerase chain reaction Methods 0.000 description 5
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 5
238000013519 translation Methods 0.000 description 5
241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 4
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
102000035101 Aspartic proteases Human genes 0.000 description 4
108091005502 Aspartic proteases Proteins 0.000 description 4
241000194108 Bacillus licheniformis Species 0.000 description 4
101900315840 Bacillus subtilis Alpha-amylase Proteins 0.000 description 4
SRBFZHDQGSBBOR-SOOFDHNKSA-N D-ribopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@@H]1O SRBFZHDQGSBBOR-SOOFDHNKSA-N 0.000 description 4
101710088194 Dehydrogenase Proteins 0.000 description 4
108030001574 Deuterolysin Proteins 0.000 description 4
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
108090000769 Isomerases Proteins 0.000 description 4
102000004195 Isomerases Human genes 0.000 description 4
241000228143 Penicillium Species 0.000 description 4
241000222350 Pleurotus Species 0.000 description 4
108020004511 Recombinant DNA Proteins 0.000 description 4
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 4
108090000787 Subtilisin Proteins 0.000 description 4
102000014701 Transketolase Human genes 0.000 description 4
108010043652 Transketolase Proteins 0.000 description 4
IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 description 4
239000002253 acid Substances 0.000 description 4
235000006708 antioxidants Nutrition 0.000 description 4
230000004071 biological effect Effects 0.000 description 4
230000002255 enzymatic effect Effects 0.000 description 4
239000000446 fuel Substances 0.000 description 4
108020001507 fusion proteins Proteins 0.000 description 4
102000037865 fusion proteins Human genes 0.000 description 4
238000002744 homologous recombination Methods 0.000 description 4
230000006801 homologous recombination Effects 0.000 description 4
238000010369 molecular cloning Methods 0.000 description 4
238000002703 mutagenesis Methods 0.000 description 4
231100000350 mutagenesis Toxicity 0.000 description 4
230000009467 reduction Effects 0.000 description 4
230000001105 regulatory effect Effects 0.000 description 4
238000011160 research Methods 0.000 description 4
229910052708 sodium Inorganic materials 0.000 description 4
239000011734 sodium Substances 0.000 description 4
230000032258 transport Effects 0.000 description 4
DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 3
MLJYKRYCCUGBBV-YTWAJWBKSA-N 4-nitrophenyl beta-D-xyloside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1OC1=CC=C([N+]([O-])=O)C=C1 MLJYKRYCCUGBBV-YTWAJWBKSA-N 0.000 description 3
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 3
241001530056 Athelia rolfsii Species 0.000 description 3
241000223651 Aureobasidium Species 0.000 description 3
101000775727 Bacillus amyloliquefaciens Alpha-amylase Proteins 0.000 description 3
241000193388 Bacillus thuringiensis Species 0.000 description 3
229920002134 Carboxymethyl cellulose Polymers 0.000 description 3
241001509964 Coptotermes Species 0.000 description 3
GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 3
ZAQJHHRNXZUBTE-UHFFFAOYSA-N D-threo-2-Pentulose Natural products OCC(O)C(O)C(=O)CO ZAQJHHRNXZUBTE-UHFFFAOYSA-N 0.000 description 3
241000233866 Fungi Species 0.000 description 3
108700007698 Genetic Terminator Regions Proteins 0.000 description 3
241000626621 Geobacillus Species 0.000 description 3
241000222684 Grifola Species 0.000 description 3
102000004157 Hydrolases Human genes 0.000 description 3
108090000604 Hydrolases Proteins 0.000 description 3
125000000570 L-alpha-aspartyl group Chemical group [H]OC(=O)C([H])([H])[C@]([H])(N([H])[H])C(*)=O 0.000 description 3
108010064700 L-arabinitol 4-dehydrogenase Proteins 0.000 description 3
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 3
125000002842 L-seryl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])O[H] 0.000 description 3
102100030931 Ladinin-1 Human genes 0.000 description 3
241000579835 Merops Species 0.000 description 3
102000003792 Metallothionein Human genes 0.000 description 3
108090000157 Metallothionein Proteins 0.000 description 3
101710130324 NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 3
241000222393 Phanerochaete chrysosporium Species 0.000 description 3
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 3
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 3
DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
241000193448 Ruminiclostridium thermocellum Species 0.000 description 3
235000003534 Saccharomyces carlsbergensis Nutrition 0.000 description 3
241001123227 Saccharomyces pastorianus Species 0.000 description 3
HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 description 3
241000187747 Streptomyces Species 0.000 description 3
241000187392 Streptomyces griseus Species 0.000 description 3
101710135785 Subtilisin-like protease Proteins 0.000 description 3
241001313536 Thermothelomyces thermophila Species 0.000 description 3
108090000190 Thrombin Proteins 0.000 description 3
108020004530 Transaldolase Proteins 0.000 description 3
102100028601 Transaldolase Human genes 0.000 description 3
241000223259 Trichoderma Species 0.000 description 3
241000378866 Trichoderma koningii Species 0.000 description 3
TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 3
230000002378 acidificating effect Effects 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
229940097012 bacillus thuringiensis Drugs 0.000 description 3
239000006227 byproduct Substances 0.000 description 3
230000015556 catabolic process Effects 0.000 description 3
108020001778 catalytic domains Proteins 0.000 description 3
230000002759 chromosomal effect Effects 0.000 description 3
238000006731 degradation reaction Methods 0.000 description 3
235000014113 dietary fatty acids Nutrition 0.000 description 3
239000000284 extract Substances 0.000 description 3
229930195729 fatty acid Natural products 0.000 description 3
239000000194 fatty acid Substances 0.000 description 3
230000006870 function Effects 0.000 description 3
239000003550 marker Substances 0.000 description 3
238000005259 measurement Methods 0.000 description 3
150000002482 oligosaccharides Chemical class 0.000 description 3
230000003647 oxidation Effects 0.000 description 3
238000007254 oxidation reaction Methods 0.000 description 3
239000000123 paper Substances 0.000 description 3
235000011837 pasties Nutrition 0.000 description 3
238000000746 purification Methods 0.000 description 3
238000003259 recombinant expression Methods 0.000 description 3
230000003248 secreting effect Effects 0.000 description 3
239000001587 sorbitan monostearate Substances 0.000 description 3
235000011076 sorbitan monostearate Nutrition 0.000 description 3
229940035048 sorbitan monostearate Drugs 0.000 description 3
239000010907 stover Substances 0.000 description 3
239000000126 substance Substances 0.000 description 3
KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 3
229960004072 thrombin Drugs 0.000 description 3
239000000811 xylitol Substances 0.000 description 3
HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 3
229960002675 xylitol Drugs 0.000 description 3
235000010447 xylitol Nutrition 0.000 description 3
HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
IFBHRQDFSNCLOZ-RMPHRYRLSA-N 4-nitrophenyl beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C([N+]([O-])=O)C=C1 IFBHRQDFSNCLOZ-RMPHRYRLSA-N 0.000 description 2
101100163849 Arabidopsis thaliana ARS1 gene Proteins 0.000 description 2
241000228215 Aspergillus aculeatus Species 0.000 description 2
241001513093 Aspergillus awamori Species 0.000 description 2
241000892910 Aspergillus foetidus Species 0.000 description 2
241001480052 Aspergillus japonicus Species 0.000 description 2
101900127796 Aspergillus oryzae Glucoamylase Proteins 0.000 description 2
241000223678 Aureobasidium pullulans Species 0.000 description 2
108010007337 Azurin Proteins 0.000 description 2
241000193752 Bacillus circulans Species 0.000 description 2
241001328122 Bacillus clausii Species 0.000 description 2
241000193749 Bacillus coagulans Species 0.000 description 2
241000193422 Bacillus lentus Species 0.000 description 2
241000194107 Bacillus megaterium Species 0.000 description 2
241000194103 Bacillus pumilus Species 0.000 description 2
229920002498 Beta-glucan Polymers 0.000 description 2
241000193764 Brevibacillus brevis Species 0.000 description 2
239000004322 Butylated hydroxytoluene Substances 0.000 description 2
NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 2
241000589876 Campylobacter Species 0.000 description 2
101100351264 Candida albicans (strain SC5314 / ATCC MYA-2876) PDC11 gene Proteins 0.000 description 2
241000146399 Ceriporiopsis Species 0.000 description 2
241001112696 Clostridia Species 0.000 description 2
241000222511 Coprinus Species 0.000 description 2
102000005927 Cysteine Proteases Human genes 0.000 description 2
108010005843 Cysteine Proteases Proteins 0.000 description 2
102000018832 Cytochromes Human genes 0.000 description 2
108010052832 Cytochromes Proteins 0.000 description 2
102000053602 DNA Human genes 0.000 description 2
241000235035 Debaryomyces Species 0.000 description 2
102000005593 Endopeptidases Human genes 0.000 description 2
108010059378 Endopeptidases Proteins 0.000 description 2
241000194033 Enterococcus Species 0.000 description 2
102000010911 Enzyme Precursors Human genes 0.000 description 2
108010062466 Enzyme Precursors Proteins 0.000 description 2
241000588724 Escherichia coli Species 0.000 description 2
241000589565 Flavobacterium Species 0.000 description 2
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
241000223194 Fusarium culmorum Species 0.000 description 2
241000567178 Fusarium venenatum Species 0.000 description 2
241000605909 Fusobacterium Species 0.000 description 2
108700023157 Galactokinases Proteins 0.000 description 2
102000048120 Galactokinases Human genes 0.000 description 2
241000222336 Ganoderma Species 0.000 description 2
240000008397 Ganoderma lucidum Species 0.000 description 2
235000001637 Ganoderma lucidum Nutrition 0.000 description 2
241000589989 Helicobacter Species 0.000 description 2
241001480714 Humicola insolens Species 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
108010018080 L-arabinose isomerase Proteins 0.000 description 2
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
125000003440 L-leucyl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] 0.000 description 2
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
ZAQJHHRNXZUBTE-WVZVXSGGSA-N L-xylulose Chemical compound OC[C@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-WVZVXSGGSA-N 0.000 description 2
241000933069 Lachnoclostridium phytofermentans Species 0.000 description 2
241000186660 Lactobacillus Species 0.000 description 2
241000194036 Lactococcus Species 0.000 description 2
241000588653 Neisseria Species 0.000 description 2
108700026244 Open Reading Frames Proteins 0.000 description 2
240000007594 Oryza sativa Species 0.000 description 2
235000007164 Oryza sativa Nutrition 0.000 description 2
102000004316 Oxidoreductases Human genes 0.000 description 2
108090000854 Oxidoreductases Proteins 0.000 description 2
101150050255 PDC1 gene Proteins 0.000 description 2
241000194109 Paenibacillus lautus Species 0.000 description 2
241000392443 Pleurotus citrinopileatus Species 0.000 description 2
241000589516 Pseudomonas Species 0.000 description 2
239000004373 Pullulan Substances 0.000 description 2
229920001218 Pullulan Polymers 0.000 description 2
206010037888 Rash pustular Diseases 0.000 description 2
241000303962 Rhizopus delemar Species 0.000 description 2
240000005384 Rhizopus oryzae Species 0.000 description 2
235000013752 Rhizopus oryzae Nutrition 0.000 description 2
PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
235000001006 Saccharomyces cerevisiae var diastaticus Nutrition 0.000 description 2
244000206963 Saccharomyces cerevisiae var. diastaticus Species 0.000 description 2
241001407717 Saccharomyces norbensis Species 0.000 description 2
241000607142 Salmonella Species 0.000 description 2
101000928111 Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) Alcohol dehydrogenase 1 Proteins 0.000 description 2
101100097319 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ala1 gene Proteins 0.000 description 2
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
102220509100 Sphingosine 1-phosphate receptor 1_Y97W_mutation Human genes 0.000 description 2
241000191940 Staphylococcus Species 0.000 description 2
108091081024 Start codon Proteins 0.000 description 2
241000194017 Streptococcus Species 0.000 description 2
241000264435 Streptococcus dysgalactiae subsp. equisimilis Species 0.000 description 2
241000193996 Streptococcus pyogenes Species 0.000 description 2
241000194054 Streptococcus uberis Species 0.000 description 2
241001468227 Streptomyces avermitilis Species 0.000 description 2
241000187432 Streptomyces coelicolor Species 0.000 description 2
241000187398 Streptomyces lividans Species 0.000 description 2
108010056079 Subtilisins Proteins 0.000 description 2
102000005158 Subtilisins Human genes 0.000 description 2
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
241001136494 Talaromyces funiculosus Species 0.000 description 2
241001540751 Talaromyces ruber Species 0.000 description 2
241000223258 Thermomyces lanuginosus Species 0.000 description 2
241000183057 Thielavia microspora Species 0.000 description 2
241000182980 Thielavia ovispora Species 0.000 description 2
HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
241000499912 Trichoderma reesei Species 0.000 description 2
239000007983 Tris buffer Substances 0.000 description 2
102000014384 Type C Phospholipases Human genes 0.000 description 2
108010079194 Type C Phospholipases Proteins 0.000 description 2
241000082085 Verticillium <Phyllachorales> Species 0.000 description 2
241001507667 Volvariella Species 0.000 description 2
241001523965 Xylaria Species 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
101150115889 al gene Proteins 0.000 description 2
235000004279 alanine Nutrition 0.000 description 2
HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 2
WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 2
230000003321 amplification Effects 0.000 description 2
230000003078 antioxidant effect Effects 0.000 description 2
229920000617 arabinoxylan Polymers 0.000 description 2
229940054340 bacillus coagulans Drugs 0.000 description 2
230000003115 biocidal effect Effects 0.000 description 2
239000000872 buffer Substances 0.000 description 2
235000010354 butylated hydroxytoluene Nutrition 0.000 description 2
229940095259 butylated hydroxytoluene Drugs 0.000 description 2
210000004899 c-terminal region Anatomy 0.000 description 2
150000001720 carbohydrates Chemical class 0.000 description 2
235000014633 carbohydrates Nutrition 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
239000001768 carboxy methyl cellulose Substances 0.000 description 2
235000010948 carboxy methyl cellulose Nutrition 0.000 description 2
239000008112 carboxymethyl-cellulose Substances 0.000 description 2
238000006555 catalytic reaction Methods 0.000 description 2
150000001768 cations Chemical class 0.000 description 2
210000000170 cell membrane Anatomy 0.000 description 2
210000002421 cell wall Anatomy 0.000 description 2
229940106157 cellulase Drugs 0.000 description 2
235000013339 cereals Nutrition 0.000 description 2
210000000349 chromosome Anatomy 0.000 description 2
239000005547 deoxyribonucleotide Substances 0.000 description 2
125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
238000001514 detection method Methods 0.000 description 2
229940066758 endopeptidases Drugs 0.000 description 2
239000003797 essential amino acid Substances 0.000 description 2
235000020776 essential amino acid Nutrition 0.000 description 2
150000002148 esters Chemical class 0.000 description 2
229930182830 galactose Natural products 0.000 description 2
LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
101150064198 gapN gene Proteins 0.000 description 2
238000012239 gene modification Methods 0.000 description 2
230000005017 genetic modification Effects 0.000 description 2
235000013617 genetically modified food Nutrition 0.000 description 2
230000006377 glucose transport Effects 0.000 description 2
229940059442 hemicellulase Drugs 0.000 description 2
230000002573 hemicellulolytic effect Effects 0.000 description 2
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 2
229910000041 hydrogen chloride Inorganic materials 0.000 description 2
238000001727 in vivo Methods 0.000 description 2
230000002779 inactivation Effects 0.000 description 2
238000002955 isolation Methods 0.000 description 2
229940039696 lactobacillus Drugs 0.000 description 2
239000003446 ligand Substances 0.000 description 2
238000007834 ligase chain reaction Methods 0.000 description 2
239000011159 matrix material Substances 0.000 description 2
230000007246 mechanism Effects 0.000 description 2
230000002503 metabolic effect Effects 0.000 description 2
230000037353 metabolic pathway Effects 0.000 description 2
125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 2
230000004048 modification Effects 0.000 description 2
238000012986 modification Methods 0.000 description 2
238000012544 monitoring process Methods 0.000 description 2
238000002887 multiple sequence alignment Methods 0.000 description 2
238000003199 nucleic acid amplification method Methods 0.000 description 2
229920001542 oligosaccharide Polymers 0.000 description 2
230000004481 post-translational protein modification Effects 0.000 description 2
230000012846 protein folding Effects 0.000 description 2
230000004853 protein function Effects 0.000 description 2
230000002797 proteolythic effect Effects 0.000 description 2
235000019423 pullulan Nutrition 0.000 description 2
208000029561 pustule Diseases 0.000 description 2
239000011541 reaction mixture Substances 0.000 description 2
230000006798 recombination Effects 0.000 description 2
238000005215 recombination Methods 0.000 description 2
230000003362 replicative effect Effects 0.000 description 2
230000004044 response Effects 0.000 description 2
235000009566 rice Nutrition 0.000 description 2
230000028327 secretion Effects 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
239000004460 silage Substances 0.000 description 2
239000001632 sodium acetate Substances 0.000 description 2
235000017281 sodium acetate Nutrition 0.000 description 2
239000001509 sodium citrate Substances 0.000 description 2
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
239000001488 sodium phosphate Substances 0.000 description 2
229910000162 sodium phosphate Inorganic materials 0.000 description 2
230000000087 stabilizing effect Effects 0.000 description 2
229940115922 streptococcus uberis Drugs 0.000 description 2
238000005556 structure-activity relationship Methods 0.000 description 2
150000008163 sugars Chemical class 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
OCUSNPIJIZCRSZ-ZTZWCFDHSA-N (2s)-2-amino-3-methylbutanoic acid;(2s)-2-amino-4-methylpentanoic acid;(2s,3s)-2-amino-3-methylpentanoic acid Chemical compound CC(C)[C@H](N)C(O)=O.CC[C@H](C)[C@H](N)C(O)=O.CC(C)C[C@H](N)C(O)=O OCUSNPIJIZCRSZ-ZTZWCFDHSA-N 0.000 description 1
CYNAPIVXKRLDER-LBPRGKRZSA-N (2s)-2-benzamido-3-(4-hydroxy-3-nitrophenyl)propanoic acid Chemical compound C([C@@H](C(=O)O)NC(=O)C=1C=CC=CC=1)C1=CC=C(O)C([N+]([O-])=O)=C1 CYNAPIVXKRLDER-LBPRGKRZSA-N 0.000 description 1
FYGDTMLNYKFZSV-WFYNLLPOSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,3s,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-WFYNLLPOSA-N 0.000 description 1
FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 1
KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 1
LXJXRIRHZLFYRP-VKHMYHEASA-L (R)-2-Hydroxy-3-(phosphonooxy)-propanal Natural products O=C[C@H](O)COP([O-])([O-])=O LXJXRIRHZLFYRP-VKHMYHEASA-L 0.000 description 1
RNAMYOYQYRYFQY-UHFFFAOYSA-N 2-(4,4-difluoropiperidin-1-yl)-6-methoxy-n-(1-propan-2-ylpiperidin-4-yl)-7-(3-pyrrolidin-1-ylpropoxy)quinazolin-4-amine Chemical compound N1=C(N2CCC(F)(F)CC2)N=C2C=C(OCCCN3CCCC3)C(OC)=CC2=C1NC1CCN(C(C)C)CC1 RNAMYOYQYRYFQY-UHFFFAOYSA-N 0.000 description 1
DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 description 1
102220633560 39S ribosomal protein S30, mitochondrial_A91L_mutation Human genes 0.000 description 1
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
101710163881 5,6-dihydroxyindole-2-carboxylic acid oxidase Proteins 0.000 description 1
244000215068 Acacia senegal Species 0.000 description 1
241000222518 Agaricus Species 0.000 description 1
235000001674 Agaricus brunnescens Nutrition 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 description 1
101710193111 All-trans-retinol dehydrogenase [NAD(+)] ADH4 Proteins 0.000 description 1
101710199313 Alpha-L-arabinofuranosidase Proteins 0.000 description 1
241000221832 Amorphotheca resinae Species 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
102000004580 Aspartic Acid Proteases Human genes 0.000 description 1
108010017640 Aspartic Acid Proteases Proteins 0.000 description 1
101001065065 Aspergillus awamori Feruloyl esterase A Proteins 0.000 description 1
101000961203 Aspergillus awamori Glucoamylase Proteins 0.000 description 1
241001091016 Aspergillus denticulatus Species 0.000 description 1
241000826947 Aspergillus nidulans var. dentatus Species 0.000 description 1
241000416162 Astragalus gummifer Species 0.000 description 1
108090001008 Avidin Proteins 0.000 description 1
108090000145 Bacillolysin Proteins 0.000 description 1
241000193747 Bacillus firmus Species 0.000 description 1
241000770536 Bacillus thermophilus Species 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
229920002749 Bacterial cellulose Polymers 0.000 description 1
108091005658 Basic proteases Proteins 0.000 description 1
102100030981 Beta-alanine-activating enzyme Human genes 0.000 description 1
102100032487 Beta-mannosidase Human genes 0.000 description 1
101710204694 Beta-xylosidase Proteins 0.000 description 1
239000002028 Biomass Substances 0.000 description 1
241000680806 Blastobotrys adeninivorans Species 0.000 description 1
241001465180 Botrytis Species 0.000 description 1
241000186146 Brevibacterium Species 0.000 description 1
241000589562 Brucella Species 0.000 description 1
101150087048 CYB2 gene Proteins 0.000 description 1
101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 1
101100005620 Caenorhabditis elegans cyb-3 gene Proteins 0.000 description 1
102220531031 Calcium/calmodulin-dependent protein kinase type II subunit gamma_S36P_mutation Human genes 0.000 description 1
101100480861 Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) tdh gene Proteins 0.000 description 1
101100439285 Candida albicans (strain SC5314 / ATCC MYA-2876) CLB4 gene Proteins 0.000 description 1
101100083070 Candida albicans (strain SC5314 / ATCC MYA-2876) PGA6 gene Proteins 0.000 description 1
101100447466 Candida albicans (strain WO-1) TDH1 gene Proteins 0.000 description 1
102100037633 Centrin-3 Human genes 0.000 description 1
241000221955 Chaetomium Species 0.000 description 1
241001057137 Chaetomium fimeti Species 0.000 description 1
241000862992 Chondromyces Species 0.000 description 1
241001674001 Chrysosporium tropicum Species 0.000 description 1
108020004638 Circular DNA Proteins 0.000 description 1
241000221760 Claviceps Species 0.000 description 1
241001509321 Clostridium thermoamylolyticum Species 0.000 description 1
229920000742 Cotton Polymers 0.000 description 1
RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 description 1
FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
LXJXRIRHZLFYRP-VKHMYHEASA-N D-glyceraldehyde 3-phosphate Chemical compound O=C[C@H](O)COP(O)(O)=O LXJXRIRHZLFYRP-VKHMYHEASA-N 0.000 description 1
RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 description 1
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
ZAQJHHRNXZUBTE-NQXXGFSBSA-N D-ribulose Chemical compound OC[C@@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-NQXXGFSBSA-N 0.000 description 1
108030006102 D-xylose reductases Proteins 0.000 description 1
125000000214 D-xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 description 1
FNZLKVNUWIIPSJ-RFZPGFLSSA-N D-xylulose 5-phosphate Chemical compound OCC(=O)[C@@H](O)[C@H](O)COP(O)(O)=O FNZLKVNUWIIPSJ-RFZPGFLSSA-N 0.000 description 1
239000003298 DNA probe Substances 0.000 description 1
241000189559 Dichomitus Species 0.000 description 1
108090000194 Dipeptidyl-peptidases and tripeptidyl-peptidases Proteins 0.000 description 1
102000003779 Dipeptidyl-peptidases and tripeptidyl-peptidases Human genes 0.000 description 1
241000123589 Dipsacus Species 0.000 description 1
241000195634 Dunaliella Species 0.000 description 1
101150015836 ENO1 gene Proteins 0.000 description 1
108010067770 Endopeptidase K Proteins 0.000 description 1
108090000371 Esterases Proteins 0.000 description 1
241000369543 Fibroporia radiculosa Species 0.000 description 1
241000192125 Firmicutes Species 0.000 description 1
229930091371 Fructose Natural products 0.000 description 1
239000005715 Fructose Substances 0.000 description 1
241000223195 Fusarium graminearum Species 0.000 description 1
241000146406 Fusarium heterosporum Species 0.000 description 1
241000223221 Fusarium oxysporum Species 0.000 description 1
101150094690 GAL1 gene Proteins 0.000 description 1
101150038242 GAL10 gene Proteins 0.000 description 1
108010093031 Galactosidases Proteins 0.000 description 1
102000002464 Galactosidases Human genes 0.000 description 1
102100028501 Galanin peptides Human genes 0.000 description 1
108010001498 Galectin 1 Proteins 0.000 description 1
102100021736 Galectin-1 Human genes 0.000 description 1
102100024637 Galectin-10 Human genes 0.000 description 1
102100028652 Gamma-enolase Human genes 0.000 description 1
206010064571 Gene mutation Diseases 0.000 description 1
101100080316 Geobacillus stearothermophilus nprT gene Proteins 0.000 description 1
241000123332 Gloeophyllum Species 0.000 description 1
241000123313 Gloeophyllum sepiarium Species 0.000 description 1
241001492300 Gloeophyllum trabeum Species 0.000 description 1
229920001503 Glucan Polymers 0.000 description 1
108010060309 Glucuronidase Proteins 0.000 description 1
102000053187 Glucuronidase Human genes 0.000 description 1
108010056007 Glyceraldehyde-3-Phosphate Dehydrogenase (NADP+)(Phosphorylating) Proteins 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
240000001080 Grifola frondosa Species 0.000 description 1
235000007710 Grifola frondosa Nutrition 0.000 description 1
229920002907 Guar gum Polymers 0.000 description 1
229920000084 Gum arabic Polymers 0.000 description 1
101150009006 HIS3 gene Proteins 0.000 description 1
241000606788 Haemophilus haemolyticus Species 0.000 description 1
101100246753 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) pyrF gene Proteins 0.000 description 1
241000969591 Haploporus papyraceus Species 0.000 description 1
101000773364 Homo sapiens Beta-alanine-activating enzyme Proteins 0.000 description 1
101000880522 Homo sapiens Centrin-3 Proteins 0.000 description 1
101000882901 Homo sapiens Claudin-2 Proteins 0.000 description 1
101100121078 Homo sapiens GAL gene Proteins 0.000 description 1
101001058231 Homo sapiens Gamma-enolase Proteins 0.000 description 1
101000579123 Homo sapiens Phosphoglycerate kinase 1 Proteins 0.000 description 1
241000223199 Humicola grisea Species 0.000 description 1
239000004354 Hydroxyethyl cellulose Substances 0.000 description 1
241000411968 Ilyobacter Species 0.000 description 1
244000017020 Ipomoea batatas Species 0.000 description 1
235000002678 Ipomoea batatas Nutrition 0.000 description 1
101710172072 Kexin Proteins 0.000 description 1
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
DWPCPZJAHOETAG-IMJSIDKUSA-N L-lanthionine Chemical compound OC(=O)[C@@H](N)CSC[C@H](N)C(O)=O DWPCPZJAHOETAG-IMJSIDKUSA-N 0.000 description 1
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
125000001176 L-lysyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C([H])([H])C([H])([H])C([H])([H])C(N([H])[H])([H])[H] 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
ZAQJHHRNXZUBTE-UCORVYFPSA-N L-ribulose Chemical compound OC[C@H](O)[C@H](O)C(=O)CO ZAQJHHRNXZUBTE-UCORVYFPSA-N 0.000 description 1
125000000769 L-threonyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])[C@](O[H])(C([H])([H])[H])[H] 0.000 description 1
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
125000003798 L-tyrosyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C([H])([H])C1=C([H])C([H])=C(O[H])C([H])=C1[H] 0.000 description 1
125000003580 L-valyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(C([H])([H])[H])(C([H])([H])[H])[H] 0.000 description 1
108010029541 Laccase Proteins 0.000 description 1
241000235087 Lachancea kluyveri Species 0.000 description 1
240000000599 Lentinula edodes Species 0.000 description 1
235000001715 Lentinula edodes Nutrition 0.000 description 1
240000007472 Leucaena leucocephala Species 0.000 description 1
235000010643 Leucaena leucocephala Nutrition 0.000 description 1
241000138839 Leucopaxillus giganteus Species 0.000 description 1
241001358029 Leucotrichum Species 0.000 description 1
229920002097 Lichenin Polymers 0.000 description 1
108090001060 Lipase Proteins 0.000 description 1
102000004882 Lipase Human genes 0.000 description 1
239000004367 Lipase Substances 0.000 description 1
241001149698 Lipomyces Species 0.000 description 1
241001480022 Lipomyces kononenkoae Species 0.000 description 1
229920000161 Locust bean gum Polymers 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
108091075413 M28E family Proteins 0.000 description 1
101150068888 MET3 gene Proteins 0.000 description 1
PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
108010054377 Mannosidases Proteins 0.000 description 1
102000001696 Mannosidases Human genes 0.000 description 1
241001184659 Melanocarpus albomyces Species 0.000 description 1
241000123315 Meripilus Species 0.000 description 1
241000123318 Meripilus giganteus Species 0.000 description 1
108090000131 Metalloendopeptidases Proteins 0.000 description 1
102000003843 Metalloendopeptidases Human genes 0.000 description 1
229920000168 Microcrystalline cellulose Polymers 0.000 description 1
102000008109 Mixed Function Oxygenases Human genes 0.000 description 1
108010074633 Mixed Function Oxygenases Proteins 0.000 description 1
101100313266 Mus musculus Tead1 gene Proteins 0.000 description 1
238000005481 NMR spectroscopy Methods 0.000 description 1
241000221961 Neurospora crassa Species 0.000 description 1
101100062121 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cyc-1 gene Proteins 0.000 description 1
101100022915 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cys-11 gene Proteins 0.000 description 1
241000208125 Nicotiana Species 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
239000006057 Non-nutritive feed additive Substances 0.000 description 1
108091028043 Nucleic acid sequence Proteins 0.000 description 1
241001072230 Oceanobacillus Species 0.000 description 1
241000272445 Ortalis Species 0.000 description 1
MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 1
KJWZYMMLVHIVSU-IYCNHOCDSA-N PGK1 Chemical compound CCCCC[C@H](O)\C=C\[C@@H]1[C@@H](CCCCCCC(O)=O)C(=O)CC1=O KJWZYMMLVHIVSU-IYCNHOCDSA-N 0.000 description 1
238000013494 PH determination Methods 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
241000863503 Paederia Species 0.000 description 1
102100026367 Pancreatic alpha-amylase Human genes 0.000 description 1
206010034133 Pathogen resistance Diseases 0.000 description 1
241001019469 Penicillium antarcticum Species 0.000 description 1
241000228129 Penicillium janthinellum Species 0.000 description 1
241000123255 Peniophora Species 0.000 description 1
102000002508 Peptide Elongation Factors Human genes 0.000 description 1
108010068204 Peptide Elongation Factors Proteins 0.000 description 1
241000222385 Phanerochaete Species 0.000 description 1
241000972672 Phellodendron Species 0.000 description 1
102000011755 Phosphoglycerate Kinase Human genes 0.000 description 1
102100028251 Phosphoglycerate kinase 1 Human genes 0.000 description 1
244000252132 Pleurotus eryngii Species 0.000 description 1
235000001681 Pleurotus eryngii Nutrition 0.000 description 1
239000004698 Polyethylene Substances 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
208000020584 Polyploidy Diseases 0.000 description 1
241000222640 Polyporus Species 0.000 description 1
244000197580 Poria cocos Species 0.000 description 1
235000008599 Poria cocos Nutrition 0.000 description 1
241000219295 Portulaca Species 0.000 description 1
101710118538 Protease Proteins 0.000 description 1
241001167522 Punctularia strigosozonata Species 0.000 description 1
101710148480 Putative beta-xylosidase Proteins 0.000 description 1
241000222644 Pycnoporus <fungus> Species 0.000 description 1
101710081551 Pyrolysin Proteins 0.000 description 1
108020004518 RNA Probes Proteins 0.000 description 1
239000003391 RNA probe Substances 0.000 description 1
101150012328 RPL18-B gene Proteins 0.000 description 1
241000959173 Rasamsonia emersonii Species 0.000 description 1
241000235525 Rhizomucor pusillus Species 0.000 description 1
241000235527 Rhizopus Species 0.000 description 1
241000593344 Rhizopus microsporus Species 0.000 description 1
101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 description 1
101100010928 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) tuf gene Proteins 0.000 description 1
241000235072 Saccharomyces bayanus Species 0.000 description 1
101100166584 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) CCW12 gene Proteins 0.000 description 1
101100507956 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) HXT7 gene Proteins 0.000 description 1
101100196145 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) RPL20B gene Proteins 0.000 description 1
101900354623 Saccharomyces cerevisiae Galactokinase Proteins 0.000 description 1
241000204893 Saccharomyces douglasii Species 0.000 description 1
241000235004 Saccharomycopsis fibuligera Species 0.000 description 1
241000209051 Saccharum Species 0.000 description 1
240000000111 Saccharum officinarum Species 0.000 description 1
235000007201 Saccharum officinarum Nutrition 0.000 description 1
101000832889 Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) Alcohol dehydrogenase 2 Proteins 0.000 description 1
101100303045 Schizosaccharomyces pombe (strain 972 / ATCC 24843) rpl1802 gene Proteins 0.000 description 1
101100022918 Schizosaccharomyces pombe (strain 972 / ATCC 24843) sua1 gene Proteins 0.000 description 1
241001123650 Schwanniomyces occidentalis Species 0.000 description 1
240000007660 Sechium edule Species 0.000 description 1
235000019095 Sechium edule Nutrition 0.000 description 1
241001085826 Sporotrichum Species 0.000 description 1
241000194048 Streptococcus equi Species 0.000 description 1
241000958303 Streptomyces achromogenes Species 0.000 description 1
241000142915 Streptomyces diastatochromogenes Species 0.000 description 1
208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 1
206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
108010062276 T-Cell Acute Lymphocytic Leukemia Protein 1 Proteins 0.000 description 1
102000011768 T-Cell Acute Lymphocytic Leukemia Protein 1 Human genes 0.000 description 1
101150001810 TEAD1 gene Proteins 0.000 description 1
101150074253 TEF1 gene Proteins 0.000 description 1
241001215623 Talaromyces cellulolyticus Species 0.000 description 1
241001484137 Talaromyces leycettanus Species 0.000 description 1
241000228182 Thermoascus aurantiacus Species 0.000 description 1
241000203780 Thermobifida fusca Species 0.000 description 1
241001136490 Thermomyces dupontii Species 0.000 description 1
101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 description 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
239000004473 Threonine Substances 0.000 description 1
241001149964 Tolypocladium Species 0.000 description 1
229920001615 Tragacanth Polymers 0.000 description 1
241000222354 Trametes Species 0.000 description 1
241001230654 Trametes cingulata Species 0.000 description 1
241000042002 Trametes sanguinea Species 0.000 description 1
241000222355 Trametes versicolor Species 0.000 description 1
101710118574 Trehalose-6-phosphate hydrolase Proteins 0.000 description 1
108700015934 Triose-phosphate isomerases Proteins 0.000 description 1
108010039203 Tripeptidyl-Peptidase 1 Proteins 0.000 description 1
102100034197 Tripeptidyl-peptidase 1 Human genes 0.000 description 1
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
101150050575 URA3 gene Proteins 0.000 description 1
108091023045 Untranslated Region Proteins 0.000 description 1
241000202898 Ureaplasma Species 0.000 description 1
241000700605 Viruses Species 0.000 description 1
229930003268 Vitamin C Natural products 0.000 description 1
241001619444 Wolfiporia cocos Species 0.000 description 1
238000004097 X-ray Buerger Methods 0.000 description 1
241000055358 Xenophora Species 0.000 description 1
229920002000 Xyloglucan Polymers 0.000 description 1
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
235000010489 acacia gum Nutrition 0.000 description 1
239000000205 acacia gum Substances 0.000 description 1
108010093941 acetylxylan esterase Proteins 0.000 description 1
238000010306 acid treatment Methods 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
238000012867 alanine scanning Methods 0.000 description 1
230000001476 alcoholic effect Effects 0.000 description 1
SRBFZHDQGSBBOR-STGXQOJASA-N alpha-D-lyxopyranose Chemical compound O[C@@H]1CO[C@H](O)[C@@H](O)[C@H]1O SRBFZHDQGSBBOR-STGXQOJASA-N 0.000 description 1
230000004075 alteration Effects 0.000 description 1
230000003322 aneuploid effect Effects 0.000 description 1
208000036878 aneuploidy Diseases 0.000 description 1
239000003945 anionic surfactant Substances 0.000 description 1
101150009206 aprE gene Proteins 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
210000004507 artificial chromosome Anatomy 0.000 description 1
235000010323 ascorbic acid Nutrition 0.000 description 1
239000011668 ascorbic acid Substances 0.000 description 1
229960005070 ascorbic acid Drugs 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
238000003149 assay kit Methods 0.000 description 1
229940005348 bacillus firmus Drugs 0.000 description 1
239000005016 bacterial cellulose Substances 0.000 description 1
125000000188 beta-D-glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
108010055059 beta-Mannosidase Proteins 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
239000003139 biocide Substances 0.000 description 1
230000009141 biological interaction Effects 0.000 description 1
230000033228 biological regulation Effects 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
235000019282 butylated hydroxyanisole Nutrition 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
229920003090 carboxymethyl hydroxyethyl cellulose Polymers 0.000 description 1
239000003093 cationic surfactant Substances 0.000 description 1
230000007910 cell fusion Effects 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
108010080434 cephalosporin-C deacetylase Proteins 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical class OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
229910017052 cobalt Inorganic materials 0.000 description 1
239000010941 cobalt Substances 0.000 description 1
GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
238000004440 column chromatography Methods 0.000 description 1
238000010960 commercial process Methods 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000004132 cross linking Methods 0.000 description 1
230000021953 cytokinesis Effects 0.000 description 1
210000000172 cytosol Anatomy 0.000 description 1
230000000593 degrading effect Effects 0.000 description 1
238000002050 diffraction method Methods 0.000 description 1
235000013399 edible fruits Nutrition 0.000 description 1
238000002003 electron diffraction Methods 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
230000007071 enzymatic hydrolysis Effects 0.000 description 1
238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
238000001952 enzyme assay Methods 0.000 description 1
150000004665 fatty acids Chemical class 0.000 description 1
KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 1
235000001785 ferulic acid Nutrition 0.000 description 1
229940114124 ferulic acid Drugs 0.000 description 1
KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 1
239000000835 fiber Substances 0.000 description 1
235000012041 food component Nutrition 0.000 description 1
239000005417 food ingredient Substances 0.000 description 1
235000004515 gallic acid Nutrition 0.000 description 1
229940074391 gallic acid Drugs 0.000 description 1
239000007789 gas Substances 0.000 description 1
230000004077 genetic alteration Effects 0.000 description 1
231100000118 genetic alteration Toxicity 0.000 description 1
230000002068 genetic effect Effects 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
MNQZXJOMYWMBOU-UHFFFAOYSA-N glyceraldehyde Chemical compound OCC(O)C=O MNQZXJOMYWMBOU-UHFFFAOYSA-N 0.000 description 1
229930182470 glycoside Natural products 0.000 description 1
150000002338 glycosides Chemical class 0.000 description 1
230000013595 glycosylation Effects 0.000 description 1
238000006206 glycosylation reaction Methods 0.000 description 1
230000012010 growth Effects 0.000 description 1
235000010417 guar gum Nutrition 0.000 description 1
239000000665 guar gum Substances 0.000 description 1
229960002154 guar gum Drugs 0.000 description 1
229910001385 heavy metal Inorganic materials 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
235000019447 hydroxyethyl cellulose Nutrition 0.000 description 1
230000006872 improvement Effects 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
230000017730 intein-mediated protein splicing Effects 0.000 description 1
230000003834 intracellular effect Effects 0.000 description 1
239000001573 invertase Substances 0.000 description 1
235000011073 invertase Nutrition 0.000 description 1
229920005610 lignin Polymers 0.000 description 1
235000019421 lipase Nutrition 0.000 description 1
239000007788 liquid Substances 0.000 description 1
235000010420 locust bean gum Nutrition 0.000 description 1
239000000711 locust bean gum Substances 0.000 description 1
229910052748 manganese Inorganic materials 0.000 description 1
239000011572 manganese Substances 0.000 description 1
FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
230000013011 mating Effects 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
DWPCPZJAHOETAG-UHFFFAOYSA-N meso-lanthionine Natural products OC(=O)C(N)CSCC(N)C(O)=O DWPCPZJAHOETAG-UHFFFAOYSA-N 0.000 description 1
230000006680 metabolic alteration Effects 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
229910021645 metal ion Inorganic materials 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
229920000609 methyl cellulose Polymers 0.000 description 1
239000001923 methylcellulose Substances 0.000 description 1
230000002906 microbiologic effect Effects 0.000 description 1
229940016286 microcrystalline cellulose Drugs 0.000 description 1
235000019813 microcrystalline cellulose Nutrition 0.000 description 1
239000008108 microcrystalline cellulose Substances 0.000 description 1
210000001724 microfibril Anatomy 0.000 description 1
230000007935 neutral effect Effects 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
239000002736 nonionic surfactant Substances 0.000 description 1
239000012038 nucleophile Substances 0.000 description 1
230000000269 nucleophilic effect Effects 0.000 description 1
230000008520 organization Effects 0.000 description 1
238000002823 phage display Methods 0.000 description 1
JTJMJGYZQZDUJJ-UHFFFAOYSA-N phencyclidine Chemical compound C1CCCCN1C1(C=2C=CC=CC=2)CCCCC1 JTJMJGYZQZDUJJ-UHFFFAOYSA-N 0.000 description 1
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
150000003904 phospholipids Chemical class 0.000 description 1
230000026731 phosphorylation Effects 0.000 description 1
238000006366 phosphorylation reaction Methods 0.000 description 1
238000005222 photoaffinity labeling Methods 0.000 description 1
238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
229920000223 polyglycerol Polymers 0.000 description 1
229920002704 polyhistidine Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
102000054765 polymorphisms of proteins Human genes 0.000 description 1
230000001124 posttranscriptional effect Effects 0.000 description 1
229920001592 potato starch Polymers 0.000 description 1
239000002243 precursor Substances 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
238000012545 processing Methods 0.000 description 1
230000004952 protein activity Effects 0.000 description 1
230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
102220264795 rs11552823 Human genes 0.000 description 1
102200153322 rs116840817 Human genes 0.000 description 1
102220005204 rs63750783 Human genes 0.000 description 1
102220123717 rs759057581 Human genes 0.000 description 1
102220072463 rs761950155 Human genes 0.000 description 1
102220239115 rs779234287 Human genes 0.000 description 1
102220085652 rs864309566 Human genes 0.000 description 1
102200153318 rs913934445 Human genes 0.000 description 1
102220246999 rs939722027 Human genes 0.000 description 1
230000007017 scission Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
238000002741 site-directed mutagenesis Methods 0.000 description 1
239000007787 solid Substances 0.000 description 1
229960002920 sorbitol Drugs 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
238000003860 storage Methods 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
230000002195 synergetic effect Effects 0.000 description 1
101150088047 tdh3 gene Proteins 0.000 description 1
238000012360 testing method Methods 0.000 description 1
235000010487 tragacanth Nutrition 0.000 description 1
239000000196 tragacanth Substances 0.000 description 1
229940116362 tragacanth Drugs 0.000 description 1
QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
230000014616 translation Effects 0.000 description 1
108010039189 tripeptidyl-peptidase 2 Proteins 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
235000019154 vitamin C Nutrition 0.000 description 1
239000011718 vitamin C Substances 0.000 description 1
238000012070 whole genome sequencing analysis Methods 0.000 description 1
238000002424 x-ray crystallography Methods 0.000 description 1
229920001285 xanthan gum Polymers 0.000 description 1
235000010493 xanthan gum Nutrition 0.000 description 1
239000000230 xanthan gum Substances 0.000 description 1
229940082509 xanthan gum Drugs 0.000 description 1
229920001221 xylan Polymers 0.000 description 1
150000004823 xylans Chemical class 0.000 description 1
229910052725 zinc Inorganic materials 0.000 description 1
239000011701 zinc Substances 0.000 description 1
FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1