CN115667929A - 检体中的目标分子的检测方法以及目标分子检测试剂盒 - Google Patents

检体中的目标分子的检测方法以及目标分子检测试剂盒 Download PDF

Info

Publication number
CN115667929A
CN115667929A CN202180036099.9A CN202180036099A CN115667929A CN 115667929 A CN115667929 A CN 115667929A CN 202180036099 A CN202180036099 A CN 202180036099A CN 115667929 A CN115667929 A CN 115667929A
Authority
CN
China
Prior art keywords
atp
molecule
reaction
target molecule
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180036099.9A
Other languages
English (en)
Chinese (zh)
Inventor
秋吉竜太郎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yokogawa Electric Corp
Original Assignee
Yokogawa Electric Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yokogawa Electric Corp filed Critical Yokogawa Electric Corp
Publication of CN115667929A publication Critical patent/CN115667929A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/0104—Pyruvate kinase (2.7.1.40)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02001—Acetate kinase (2.7.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04001—Polyphosphate kinase (2.7.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04003—Adenylate kinase (2.7.4.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07004—Sulfate adenylyltransferase (2.7.7.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/09—Phosphotransferases with paired acceptors (2.7.9)
    • C12Y207/09001—Pyruvate, phosphate dikinase (2.7.9.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01001—Acetate-CoA ligase (6.2.1.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/548—Carbohydrates, e.g. dextran
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2470/00—Immunochemical assays or immunoassays characterised by the reaction format or reaction type
    • G01N2470/04—Sandwich assay format

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202180036099.9A 2020-05-25 2021-05-21 检体中的目标分子的检测方法以及目标分子检测试剂盒 Pending CN115667929A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020090398 2020-05-25
JP2020-090398 2020-05-25
PCT/JP2021/019390 WO2021241446A1 (ja) 2020-05-25 2021-05-21 検体中の標的分子の検出方法、及び標的分子検出キット

Publications (1)

Publication Number Publication Date
CN115667929A true CN115667929A (zh) 2023-01-31

Family

ID=78744721

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180036099.9A Pending CN115667929A (zh) 2020-05-25 2021-05-21 检体中的目标分子的检测方法以及目标分子检测试剂盒

Country Status (6)

Country Link
US (1) US20230131011A1 (https=)
EP (1) EP4159755A4 (https=)
JP (1) JP7226651B2 (https=)
KR (1) KR102833537B1 (https=)
CN (1) CN115667929A (https=)
WO (1) WO2021241446A1 (https=)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5246834A (en) * 1991-02-21 1993-09-21 Kikkoman Corporation Enzyme immunoassay method employing acetate kinase
JP2001299390A (ja) * 2000-04-20 2001-10-30 Satake Corp Ａｔｐを連鎖的に増幅させる方法及び該方法を利用して極微量のａｔｐを検査する方法
CN1395619A (zh) * 2000-01-17 2003-02-05 株式会社佐竹 Atp再生反应系统、利用该系统的腺苷酸的检验方法、rna检测方法和atp放大方法
JP2009060809A (ja) * 2007-09-04 2009-03-26 Hiroshima Univ アデノシン三リン酸を増幅させるための反応器およびアデノシン三リン酸の増幅方法、並びにその利用
US20090162881A1 (en) * 2005-11-14 2009-06-25 Matsushita Electric Industrial Co., Ltd. Method of measuring adenine nucleotide

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2055200B (en) * 1979-07-24 1984-05-02 Kolehmainen S Luminescent assays with enzymatic cycling enhancement of the sensitivity of bioluminescent and chemi
JPS59166099A (ja) 1983-03-11 1984-09-19 Toyo Jozo Co Ltd Ａｔｐまたはｎｍｎの高感度測定法
JPS6311848A (ja) 1986-07-02 1988-01-19 Oriental Yeast Co Ltd アデノシン３リン酸関連化合物の簡易測定法
JPS6423900A (en) 1987-07-16 1989-01-26 Sankyo Co Method for determining atp by method for enzymic amplification
JPH04360700A (ja) 1991-06-01 1992-12-14 Canon Inc Ａｔｐ測定用テスター
JP3072190B2 (ja) * 1992-09-03 2000-07-31 キッコーマン株式会社 酵素免疫測定法
GB9414096D0 (en) * 1994-07-13 1994-08-31 Secr Defence Labelled capture assay
JP3409962B2 (ja) 1996-03-04 2003-05-26 キッコーマン株式会社 生物発光試薬及びその試薬を用いたアデノシンリン酸エステルの定量法並びにその試薬を用いたａｔｐ変換反応系に関与する物質の定量法
GB9902659D0 (en) 1999-02-05 1999-03-31 Microbiological Res Authority Assay with reduced background
AU2002367744A1 (en) 2001-07-03 2003-10-27 The Board Of Trustees Of The Leland Stanford University Bioluminescence regenerative cycle (brc) for nucleic acid quantification
JP2003225098A (ja) 2002-02-04 2003-08-12 Unitika Ltd 土壌微生物数の測定方法およびその試薬
JP2006081506A (ja) 2004-09-17 2006-03-30 Hiroshima Univ 微生物の高感度迅速検出方法
JP5245492B2 (ja) 2008-03-27 2013-07-24 ブラザー工業株式会社 インクジェット記録装置
JP5871224B2 (ja) 2011-08-11 2016-03-01 国立大学法人豊橋技術科学大学 細胞放出物質検出装置、細胞放出物質検出方法および細胞放出物質検出用固定化酵素基板
JP6295408B2 (ja) 2013-08-26 2018-03-20 国立大学法人北海道大学 血液検体のａｔｐ測定方法及びキット
JP7328763B2 (ja) 2017-02-09 2023-08-17 キッコーマン株式会社 生体関連サンプル及び生体関連器具の清浄度測定キット及び方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5246834A (en) * 1991-02-21 1993-09-21 Kikkoman Corporation Enzyme immunoassay method employing acetate kinase
CN1395619A (zh) * 2000-01-17 2003-02-05 株式会社佐竹 Atp再生反应系统、利用该系统的腺苷酸的检验方法、rna检测方法和atp放大方法
JP2001299390A (ja) * 2000-04-20 2001-10-30 Satake Corp Ａｔｐを連鎖的に増幅させる方法及び該方法を利用して極微量のａｔｐを検査する方法
US20090162881A1 (en) * 2005-11-14 2009-06-25 Matsushita Electric Industrial Co., Ltd. Method of measuring adenine nucleotide
JP2009060809A (ja) * 2007-09-04 2009-03-26 Hiroshima Univ アデノシン三リン酸を増幅させるための反応器およびアデノシン三リン酸の増幅方法、並びにその利用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KATSUTOSHI ITO 等: "Novel bioluminescent assay of pyruvate phosphate dikinase using firefly luciferase–luciferin reaction and its application to bioluminescent enzyme immunoassay", 《ANALYTICA CHIMICA ACTA》, vol. 421, 31 December 2000 (2000-12-31), pages 113 *
KATSUTOSHI ITO等: "Highly Sensitive Simultaneous Bioluminescent Measurement of Acetate Kinase and Pyruvate Phosphate Dikinase Activities using a Firefly Luciferase-Luciferin Reaction and Its Application to a Tandem Bioluminescent Enzyme Immunoassay", 《ANALYTICAL SCIENCES》, vol. 19, no. 1, 31 January 2003 (2003-01-31), pages 105 - 109 *

Also Published As

Publication number Publication date
EP4159755A1 (en) 2023-04-05
JPWO2021241446A1 (https=) 2021-12-02
EP4159755A4 (en) 2024-07-31
KR102833537B1 (ko) 2025-07-11
JP7226651B2 (ja) 2023-02-21
KR20220167333A (ko) 2022-12-20
US20230131011A1 (en) 2023-04-27
WO2021241446A1 (ja) 2021-12-02

Similar Documents

Publication Publication Date Title
US20250060361A1 (en) Assay methods
Kricka Application of bioluminescence and chemiluminescence in biomedical sciences
RU2199125C2 (ru) Меченное люциферазой антитело и способ его получения, способ осуществления анализа на специфическое связывание и набор для применения в анализе на специфическое связывание
US20050112601A1 (en) Methods for cellular or microorganism capture and quantification using bioluminescence regenerative cycle (BRC) assays
US8492101B2 (en) Chemiluminescent enzyme assay method and apparatus
CN108152505A (zh) 免疫测定方法、用于鉴定免疫测定的系统和试剂盒
US5246834A (en) Enzyme immunoassay method employing acetate kinase
JP7226651B2 (ja) 検体中の標的分子の検出方法、及び標的分子検出キット
Ito et al. Highly sensitive simultaneous bioluminescent measurement of acetate kinase and pyruvate phosphate dikinase activities using a firefly luciferase-luciferin reaction and its application to a tandem bioluminescent enzyme immunoassay
US20240003891A1 (en) Immunoassay using carbon nanomaterials and method of detecting target antigen using the same
CN105467113A (zh) 一种基于荧光素和萤光素酶生物发光反应的免疫分析方法
Minekawa et al. Practical application of bioluminescence enzyme immunoassay using enhancer for firefly luciferin–luciferase bioluminescence
JP3072190B2 (ja) 酵素免疫測定法
JPH10239314A (ja) 結合分析の高感度化方法
CN117999482A (zh) 用于测定信号扩增的方法、组合物和试剂盒
HK40015589A (en) Improved assay methods
ITO et al. School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan Email: itok@ pharm. showa-u. ac. jp
ITO et al. DEVELOPMENT OF SIMULTANEOUS BIOLUMINESCENT ASSAY OF ACETATE KINASE AND PYRUVATE PHOSPHATE DIKINASE USING FIREFLY LUCIFERASE-LUCIFERIN REACTION
Lee et al. Research Article Single-Molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Based on Evanescent Field-Enhanced Fluorescence Imaging
JPH03112496A (ja) 物質の定量分析用標識剤

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination