JPS6423900A - Method for determining atp by method for enzymic amplification - Google Patents
Method for determining atp by method for enzymic amplificationInfo
- Publication number
- JPS6423900A JPS6423900A JP17794587A JP17794587A JPS6423900A JP S6423900 A JPS6423900 A JP S6423900A JP 17794587 A JP17794587 A JP 17794587A JP 17794587 A JP17794587 A JP 17794587A JP S6423900 A JPS6423900 A JP S6423900A
- Authority
- JP
- Japan
- Prior art keywords
- atp
- glucose
- phosphate
- converted
- determined
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
PURPOSE:To stably determine adenosine triphosphate (ATP) with a high sensitivity and accuracy, by using hexokinase and pyruvate kinase together, amplifying reaction forming glucose 6-phosphate from D-glucose with a specific chemiluminescence reagent. CONSTITUTION:ATP is determined. In the process, D-glucose which is a substrate is converted into glucose 6-phosphate and the ATP is simultaneously converted into adenosine diphosphate (ADP), which is then converted into ATP with pyruvate kinase. Thereby oxidative-reductive reaction in which the ATP is infinitely rotated in principle to integrate the glucose 6-phosphate that is decomposed with glucose 6-phosphate dehydrogenase to simultaneously convert the nicotinamide adenine dinucleotide phosphate (NADP) into reduced form NADP is used to cause chemiluminescence of 1-methoxy-5-phenazinemethyl sulfate and isoluminol. The ATP is determined by an enzymic amplification method.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17794587A JPS6423900A (en) | 1987-07-16 | 1987-07-16 | Method for determining atp by method for enzymic amplification |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17794587A JPS6423900A (en) | 1987-07-16 | 1987-07-16 | Method for determining atp by method for enzymic amplification |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6423900A true JPS6423900A (en) | 1989-01-26 |
Family
ID=16039823
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP17794587A Pending JPS6423900A (en) | 1987-07-16 | 1987-07-16 | Method for determining atp by method for enzymic amplification |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6423900A (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994025619A1 (en) * | 1993-04-23 | 1994-11-10 | Celsis Limited | Detection of biological material |
WO2003044222A1 (en) * | 2001-11-21 | 2003-05-30 | Unitika Ltd. | Atp measurement method allowing visual judgement and reagent therefor |
JP2006223163A (en) * | 2005-02-16 | 2006-08-31 | Hiroshima Univ | Method for determining trace atp by atp amplifying-cycling method, and kit |
WO2018174336A1 (en) * | 2017-03-23 | 2018-09-27 | 한국과학기술원 | Method for detecting atp by using personal blood glucose meter |
WO2021241446A1 (en) | 2020-05-25 | 2021-12-02 | 横河電機株式会社 | Method for detecting target molecule in specimen, and target molecule detection kit |
-
1987
- 1987-07-16 JP JP17794587A patent/JPS6423900A/en active Pending
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994025619A1 (en) * | 1993-04-23 | 1994-11-10 | Celsis Limited | Detection of biological material |
US6043047A (en) * | 1993-04-23 | 2000-03-28 | Celsis International, Plc | Sample-collecting and assay device for use in the detection of biological material |
WO2003044222A1 (en) * | 2001-11-21 | 2003-05-30 | Unitika Ltd. | Atp measurement method allowing visual judgement and reagent therefor |
EP1445328A1 (en) * | 2001-11-21 | 2004-08-11 | Unitika Ltd. | Atp measurement method allowing visual judgement and reagent therefor |
EP1445328A4 (en) * | 2001-11-21 | 2004-12-22 | Unitika Ltd | Atp measurement method allowing visual judgement and reagent therefor |
US7122333B2 (en) | 2001-11-21 | 2006-10-17 | Unitika Ltd. | Method and reagent for visually measuring ATP |
JP2006223163A (en) * | 2005-02-16 | 2006-08-31 | Hiroshima Univ | Method for determining trace atp by atp amplifying-cycling method, and kit |
WO2018174336A1 (en) * | 2017-03-23 | 2018-09-27 | 한국과학기술원 | Method for detecting atp by using personal blood glucose meter |
WO2021241446A1 (en) | 2020-05-25 | 2021-12-02 | 横河電機株式会社 | Method for detecting target molecule in specimen, and target molecule detection kit |
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