JP7226651B2 - 検体中の標的分子の検出方法、及び標的分子検出キット - Google Patents
検体中の標的分子の検出方法、及び標的分子検出キット Download PDFInfo
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- JP7226651B2 JP7226651B2 JP2022526991A JP2022526991A JP7226651B2 JP 7226651 B2 JP7226651 B2 JP 7226651B2 JP 2022526991 A JP2022526991 A JP 2022526991A JP 2022526991 A JP2022526991 A JP 2022526991A JP 7226651 B2 JP7226651 B2 JP 7226651B2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
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- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
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- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
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- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/0104—Pyruvate kinase (2.7.1.40)
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- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
- C12Y207/02001—Acetate kinase (2.7.2.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
- C12Y207/04001—Polyphosphate kinase (2.7.4.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
- C12Y207/04003—Adenylate kinase (2.7.4.3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07004—Sulfate adenylyltransferase (2.7.7.4)
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- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/09—Phosphotransferases with paired acceptors (2.7.9)
- C12Y207/09001—Pyruvate, phosphate dikinase (2.7.9.1)
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- C12Y—ENZYMES
- C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
- C12Y602/01—Acid-Thiol Ligases (6.2.1)
- C12Y602/01001—Acetate-CoA ligase (6.2.1.1)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/548—Carbohydrates, e.g. dextran
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
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- C07—ORGANIC CHEMISTRY
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- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2470/00—Immunochemical assays or immunoassays characterised by the reaction format or reaction type
- G01N2470/04—Sandwich assay format
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- Urology & Nephrology (AREA)
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- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Food Science & Technology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020090398 | 2020-05-25 | ||
| JP2020090398 | 2020-05-25 | ||
| PCT/JP2021/019390 WO2021241446A1 (ja) | 2020-05-25 | 2021-05-21 | 検体中の標的分子の検出方法、及び標的分子検出キット |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2021241446A1 JPWO2021241446A1 (https=) | 2021-12-02 |
| JPWO2021241446A5 JPWO2021241446A5 (https=) | 2022-09-29 |
| JP7226651B2 true JP7226651B2 (ja) | 2023-02-21 |
Family
ID=78744721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022526991A Active JP7226651B2 (ja) | 2020-05-25 | 2021-05-21 | 検体中の標的分子の検出方法、及び標的分子検出キット |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20230131011A1 (https=) |
| EP (1) | EP4159755A4 (https=) |
| JP (1) | JP7226651B2 (https=) |
| KR (1) | KR102833537B1 (https=) |
| CN (1) | CN115667929A (https=) |
| WO (1) | WO2021241446A1 (https=) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001299390A (ja) | 2000-04-20 | 2001-10-30 | Satake Corp | Atpを連鎖的に増幅させる方法及び該方法を利用して極微量のatpを検査する方法 |
| JP2002535985A (ja) | 1999-02-05 | 2002-10-29 | マイクロバイオロジカル リサーチ オーソリティ | 低下させたバックグラウンドを用いるアッセイ |
| JP2006081506A (ja) | 2004-09-17 | 2006-03-30 | Hiroshima Univ | 微生物の高感度迅速検出方法 |
| JP2009060809A (ja) | 2007-09-04 | 2009-03-26 | Hiroshima Univ | アデノシン三リン酸を増幅させるための反応器およびアデノシン三リン酸の増幅方法、並びにその利用 |
| JP2013040800A (ja) | 2011-08-11 | 2013-02-28 | Toyohashi Univ Of Technology | 細胞放出物質検出装置、細胞放出物質検出方法および細胞放出物質検出用固定化酵素基板 |
| JP2015042156A (ja) | 2013-08-26 | 2015-03-05 | 国立大学法人北海道大学 | 血液検体のatp測定方法及びキット |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2055200B (en) * | 1979-07-24 | 1984-05-02 | Kolehmainen S | Luminescent assays with enzymatic cycling enhancement of the sensitivity of bioluminescent and chemi |
| JPS59166099A (ja) | 1983-03-11 | 1984-09-19 | Toyo Jozo Co Ltd | Atpまたはnmnの高感度測定法 |
| JPS6311848A (ja) | 1986-07-02 | 1988-01-19 | Oriental Yeast Co Ltd | アデノシン3リン酸関連化合物の簡易測定法 |
| JPS6423900A (en) | 1987-07-16 | 1989-01-26 | Sankyo Co | Method for determining atp by method for enzymic amplification |
| JP2856339B2 (ja) * | 1991-02-21 | 1999-02-10 | キッコーマン株式会社 | 酵素免疫測定法 |
| JPH04360700A (ja) | 1991-06-01 | 1992-12-14 | Canon Inc | Atp測定用テスター |
| JP3072190B2 (ja) * | 1992-09-03 | 2000-07-31 | キッコーマン株式会社 | 酵素免疫測定法 |
| GB9414096D0 (en) * | 1994-07-13 | 1994-08-31 | Secr Defence | Labelled capture assay |
| JP3409962B2 (ja) | 1996-03-04 | 2003-05-26 | キッコーマン株式会社 | 生物発光試薬及びその試薬を用いたアデノシンリン酸エステルの定量法並びにその試薬を用いたatp変換反応系に関与する物質の定量法 |
| EP1264894A4 (en) * | 2000-01-17 | 2005-11-30 | Satake Eng Co Ltd | REACTION SYSTEMS FOR ATP REGENERATION AND METHOD FOR THE CONTROL OF ADENIN NUCLEOTIDES, METHOD FOR THE DETECTION OF RNA AND METHOD FOR THE AMPLIFICATION OF ATP USING THEREOF |
| AU2002367744A1 (en) | 2001-07-03 | 2003-10-27 | The Board Of Trustees Of The Leland Stanford University | Bioluminescence regenerative cycle (brc) for nucleic acid quantification |
| JP2003225098A (ja) | 2002-02-04 | 2003-08-12 | Unitika Ltd | 土壌微生物数の測定方法およびその試薬 |
| WO2007055331A1 (ja) * | 2005-11-14 | 2007-05-18 | Matsushita Electric Industrial Co., Ltd. | アデニンヌクレオチドの測定方法 |
| JP5245492B2 (ja) | 2008-03-27 | 2013-07-24 | ブラザー工業株式会社 | インクジェット記録装置 |
| JP7328763B2 (ja) | 2017-02-09 | 2023-08-17 | キッコーマン株式会社 | 生体関連サンプル及び生体関連器具の清浄度測定キット及び方法 |
-
2021
- 2021-05-21 EP EP21814546.4A patent/EP4159755A4/en active Pending
- 2021-05-21 CN CN202180036099.9A patent/CN115667929A/zh active Pending
- 2021-05-21 KR KR1020227040159A patent/KR102833537B1/ko active Active
- 2021-05-21 US US17/924,748 patent/US20230131011A1/en active Pending
- 2021-05-21 JP JP2022526991A patent/JP7226651B2/ja active Active
- 2021-05-21 WO PCT/JP2021/019390 patent/WO2021241446A1/ja not_active Ceased
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002535985A (ja) | 1999-02-05 | 2002-10-29 | マイクロバイオロジカル リサーチ オーソリティ | 低下させたバックグラウンドを用いるアッセイ |
| JP2001299390A (ja) | 2000-04-20 | 2001-10-30 | Satake Corp | Atpを連鎖的に増幅させる方法及び該方法を利用して極微量のatpを検査する方法 |
| JP2006081506A (ja) | 2004-09-17 | 2006-03-30 | Hiroshima Univ | 微生物の高感度迅速検出方法 |
| JP2009060809A (ja) | 2007-09-04 | 2009-03-26 | Hiroshima Univ | アデノシン三リン酸を増幅させるための反応器およびアデノシン三リン酸の増幅方法、並びにその利用 |
| JP2013040800A (ja) | 2011-08-11 | 2013-02-28 | Toyohashi Univ Of Technology | 細胞放出物質検出装置、細胞放出物質検出方法および細胞放出物質検出用固定化酵素基板 |
| JP2015042156A (ja) | 2013-08-26 | 2015-03-05 | 国立大学法人北海道大学 | 血液検体のatp測定方法及びキット |
Non-Patent Citations (3)
| Title |
|---|
| ITO, Katsutoshi et al.,Highly Sensitive Simultaneous bioluminescent Measurement of Acetate Kinase and Pyruvate Phosphate Di,Analytical Sciences,2003年,19(1),105-109 |
| SATOH Tetsuya et al.,ATP Amplification for Ultrasensitive Bioluminescence Assay: Detection of a Single Bacterial Cell,Biosci. Biotechnol. Biochem.,2004年06月,Vol.68, No.6,pp.1216-1220 |
| 佐藤哲也ほか,連鎖的ATP増幅反応を用いた超微量微生物の検出,日本生物工学会大会講演要旨集,2003年08月25日,p.54, 2A14-3 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4159755A1 (en) | 2023-04-05 |
| JPWO2021241446A1 (https=) | 2021-12-02 |
| EP4159755A4 (en) | 2024-07-31 |
| KR102833537B1 (ko) | 2025-07-11 |
| KR20220167333A (ko) | 2022-12-20 |
| CN115667929A (zh) | 2023-01-31 |
| US20230131011A1 (en) | 2023-04-27 |
| WO2021241446A1 (ja) | 2021-12-02 |
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