CN115399239A - Interspecific cross breeding method for novel lina plant - Google Patents
Interspecific cross breeding method for novel lina plant Download PDFInfo
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- CN115399239A CN115399239A CN202211227073.5A CN202211227073A CN115399239A CN 115399239 A CN115399239 A CN 115399239A CN 202211227073 A CN202211227073 A CN 202211227073A CN 115399239 A CN115399239 A CN 115399239A
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- lina
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- lotus
- cross breeding
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000009402 cross-breeding Methods 0.000 title claims abstract description 17
- 240000002853 Nelumbo nucifera Species 0.000 claims abstract description 42
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims abstract description 39
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims abstract description 39
- 241000233805 Phoenix Species 0.000 claims abstract description 13
- 238000009403 interspecific hybridization Methods 0.000 claims abstract description 9
- 239000000758 substrate Substances 0.000 claims description 25
- 238000009331 sowing Methods 0.000 claims description 11
- 230000010152 pollination Effects 0.000 claims description 9
- 239000003415 peat Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 6
- 239000011435 rock Substances 0.000 claims description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims description 5
- 235000019354 vermiculite Nutrition 0.000 claims description 5
- 239000010455 vermiculite Substances 0.000 claims description 5
- 238000003306 harvesting Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 241000511901 Lycianthes Species 0.000 claims 2
- 241000196324 Embryophyta Species 0.000 abstract description 28
- 238000009395 breeding Methods 0.000 abstract description 9
- 241000133134 Saussurea Species 0.000 abstract description 8
- 241001091575 Echeveria Species 0.000 description 10
- 230000001488 breeding effect Effects 0.000 description 8
- 241000238631 Hexapoda Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 241000220284 Crassulaceae Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000196240 Characeae Species 0.000 description 1
- 241000167853 Linaria Species 0.000 description 1
- 241001145025 Saussurea involucrata Species 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000001969 hypertrophic effect Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention provides a method for interspecific cross breeding of a new lina plant, and belongs to the technical field of cross breeding. The breeding method of the invention comprises the steps of carrying out artificial interspecific hybridization by using the stoneleaf lotus as a female parent and the stoneleaf saussurea or the white phoenix as a male parent to generate a new hybrid variety. The invention can select the new hybrid variety of the Lina lotus series with excellent properties, the new hybrid variety can resist the low temperature of-8 ℃ and the high temperature of 40 ℃, and the ornamental value is good.
Description
Technical Field
The invention belongs to the technical field of hybrid breeding, and particularly relates to a method for interspecific hybrid breeding of a new lina plant.
Background
Succulent plants (succulent plants or succulent plants) are plants in which a part of the vegetative organs of the plant, such as the stem, leaves or roots (of a small number of species) has developed parenchyma to store water and appear hypertrophic and succulent in appearance. With the improvement of living standard of substances, people pursue more and more good things. Succulent plants are popular with people all over the world due to the characteristics of small size, exquisite posture, scoopability in bud state and the like.
Crassulaceae is the first major family of succulent plants, echeveria (echeveria.) is a major genus of Crassulaceae, the plants of the genus are common garden ornamental plants, the varieties of the garden are many, the arrangement form and the color of leaves have the characteristics of changing along with seasonal changes, the plants are shaped like flowers, and the plants are known as 'flowers which never wither', and are very popular with the public. The lina Echever lilacina is a famous succulent plant of the echeveria, the leaves of the lina echeveria are in an inverted egg shape, the edges of the leaves are slightly wavy, the leaves are very hard and perfect, the body of the lina echeveria is covered with a white box, the lina echeveria can resist the low temperature of-8 ℃, and the lina echeveria is suitable for the advantageous species of the Yangtze river basin. Saussurea involucrate Echeveria laui Moran & Meyr-ni and the white phoenix Echever 'Hakuhou' are both Echeveria succulent.
However, the stonewort succulent plants mostly belong to spring and autumn varieties, generally grow in spring and autumn, mostly like warm, dry and sunny long-day and well-ventilated living environments, are not resistant to high temperature, high humidity and severe cold, have the problems of black rot and the like caused by overhigh temperature, and are not easy to popularize and plant in high-temperature areas in summer and severe cold areas in winter.
Disclosure of Invention
In view of the above, the invention aims to provide a method for interspecific hybridization breeding of a new liinermis plant, and the new variety of the cultured echeveria hybrid integrates the excellent properties of parents, resists the low temperature of-8 ℃, resists the high temperature of 40 ℃ and has good ornamental value.
In order to achieve the above purpose, the invention provides the following technical scheme:
an interspecific hybridization method for the new Liananlia plant features that the Liananlia of pseudo-Nelumbo is used as female parent and the snow lotus or Baifeng of pseudo-Nelumbo is used as male parent for artificial interspecific hybridization to generate new hybridized variety.
Preferably, the number ratio of female parent to male parent is 1.
Preferably, the artificial interspecific hybridization comprises: bagging is carried out before the first flower of the male parent and the female parent bloom, and when the first flower of the female parent blooms, pollen of the male parent is collected and pollinated for 2 times.
More preferably, the first pollination is carried out at 8-9 and the second pollination is carried out at 15-16.
Preferably, the method also comprises the steps of harvesting, sowing and seedling management of the seeds of the new hybrid variety.
More preferably, the hybrid seed is harvested and sown into a seedling substrate after maturation.
More preferably, the seedling substrate is divided into two layers, the thickness of the lower substrate is 5-8cm, and the weight ratio of peat: volcanic rock: vermiculite =8-12, 1-3, upper substrate particle size 0.3-0.7mm, thickness 0.4-0.6cm, fine peat: fine sand = 8-12.
More preferably, after sowing, the temperature is controlled at 15-18 ℃ at night and 22-25 ℃ during the day, and the substrate is kept moist.
More preferably, the seedling management comprises: after seedling emergence, water is sprayed twice a day, and a sunshade net is covered in summer to keep ventilation.
More preferably, the spraying is carried out once in the afternoon and once in the water; the shading rate of the sunshade net is 65-75 percent; the ventilation volume is 2-3 levels.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a method for interspecific cross breeding of a new lina lotus plant, and discloses a cross breeding technical system for breeding a new hybrid variety among lina lotus of genus jacobian, saussurea involucrate and baifeng, wherein the technical system can select a new lina lotus series hybrid variety with excellent properties.
The Lina lotus seed and the saussurea involucrate are novel hybrid varieties, and the Lina lotus seed and the white phoenix are horticultural varieties, so that the novel hybrid varieties resist low temperature of-8 ℃ and high temperature of 40 ℃ and have good ornamental performance.
Drawings
FIG. 1: a. female parent lina; b. male parent 1 saussurea involucrate; c. male parent 2 white phoenix;
FIG. 2: a. lina lotus female parent and saussurea involucrate male parent hybrid; b. lina lotus male parent and white phoenix female parent hybrid 1; c. lina lotus female parent and white phoenix male parent hybrid 2.
Detailed Description
The invention provides a method for interspecific hybridization of a new lina lotus plant, which is characterized in that artificial interspecific hybridization is carried out by taking the lina lotus of the genus of pseudo-stoneleaf as a female parent and taking the snow lotus or the white phoenix of the genus of pseudo-stoneleaf as a male parent to generate a new hybrid.
In the invention, the female parent lina lotus is preferably 4-5 years potted seedling, the male parent saussurea involucrate is 4-5 years potted seedling, and the male parent Baifeng is 4-5 years potted leaf seedling; the above seedlings are further optimized, and the plants are required to grow robustly and have no plant diseases and insect pests.
The preferred number ratio of the female parent to the male parent is 1.
Lina lotus, saussurea involucrate and Baifeng are all cross-pollinated plants and usually bloom in 4-5 months. Preferred artificial interspecific crosses of the invention include: bagging the male parent and the female parent before the first flower blooms, and collecting pollen of the male parent and pollinating for 2 times when the first flower of the female parent blooms; it is further preferred that the first pollination is carried out at 8-9 and the second pollination is carried out at 15-16; more preferably, every subsequent flower is pollinated for 2 times according to the method.
The interspecific cross breeding method also preferably comprises the steps of harvesting seeds of the new hybrid variety, sowing and seedling management.
The hybridized fruits of the Lina lotus seed female parent, the snow lotus seed female parent and the Lina lotus seed male parent are generally ripe after 1 month. According to the invention, after the hybrid seeds are mature, the hybrid seeds are harvested and sowed into a seedling substrate; further preferably, when the seeds are harvested, the mature hybrid fruits are sun-dried for one week, and then the seeds are taken out and sown.
The seedling raising substrate is preferably divided into two layers, the thickness of the lower substrate is 5-8cm, the further preferable thickness is 6-7cm, and the seedling raising substrate is prepared from peat: volcanic rock: vermiculite =8-12 (volume ratio) composition, further preferably 10; the upper layer substrate has a diameter of 0.3 to 0.7mm, more preferably 0.4 to 0.6mm, more preferably 0.5mm, a thickness of 0.4 to 0.6cm, more preferably 0.5cm, and a thickness of 0.3 to 0.7mm, and is prepared from fine peat: fine sand =8-12 (volume ratio), further preferably 10. The Lina lotus hybrid seeds are extremely small, the traditional substrate is not beneficial to the emergence of seedlings, the seedling raising substrate is divided into two layers, and the upper layer is composed of a substrate with a small particle size of 0.4-0.6mm, so that the seeding and the emergence of the hybrid seeds are facilitated.
In the present invention, after sowing, the temperature at night is preferably controlled to be 15 to 18 ℃, more preferably 16 to 17 ℃, and the temperature during the day is controlled to be 22 to 25 ℃, more preferably 23 to 24 ℃, and the substrate is kept moist, more preferably the moisture content is 60%. Managing according to the above conditions, and germinating after 7-10 days of hybrid seeds.
After the seedlings are preferably selected, water is sprayed twice a day, and a sunshade net is covered in summer to keep ventilation; further preferably, the water spraying is performed once each in the afternoon, and more preferably, the water spraying is performed 1 time each at 10 am and 15 pm; the shading rate of the sunshade net is 65-75%, and more preferably 70%; the ventilation volume is 2-3 levels. As one possible implementation, the ventilation environment is ensured by the provision of a miniature ceiling fan.
The present invention is preferably transplanted after 2 months of emergence, and as an embodiment, it is transplanted using pots with diameter of 7cm x depth of 7cm, and the transplanting medium is peat: volcanic rock: vermiculite =8-12 (volume ratio) composition, further preferably 10; preferably, the seedlings are acclimatized in a regulation greenhouse 3 months in the next year.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
And (3) cross breeding of the lina lotus and the saussurea involucrata.
Taking lina lotus as a female parent (shown in figure 1 a) and snow lotus as a male parent (shown in figure 1 b), carrying out hybridization breeding, wherein the year 2020 is 4, 1 days, a plant research institute (Nanjing Zhongshan botanical garden) of Chinese academy of sciences in Jiangsu province is equipped with a greenhouse to select plants which are strong in growth and free of plant diseases and insect pests, 4-5 years of buds are planted in pots for seedlings, lina lotus and snow lotus, each ten pots of snow lotus are bagged before the first flower is opened, the lina lotus and the snow lotus start to bloom at 18 days 4, 8-9 am when one flower of lina lotus \ 33488is opened, collecting snow lotus pollen of the male parent 1 for pollination, and pollinating at 3-4 pm by using the snow lotus pollen for the second time. And harvesting mature seed fruits of the male and female Lina lotus, and taking out the seeds for sowing after the Lina lotus is sunned for one week in the sun. Sowing is carried out by using a sowing pot which is 37cm long, 30cm wide and 7cm deep, the sowing substrate comprises a lower substrate and an upper substrate, the lower substrate comprises inlet peat, volcanic rock and vermiculite according to a volume ratio of 10. The lower substrate is 5.5cm thick, and the upper substrate is 0.5cm thick. After sowing, controlling the temperature at 15-18 ℃ at night, controlling the temperature at 22-25 ℃ in daytime, and germinating for 7-10 days. Spraying water at 10 am and 3 pm after seedling emergence, shading with 70% sunshade net in summer, and turning on gentle breeze ceiling fan to regulate and control air volume by 2-3 level. After two months, the seedlings are transplanted by flowerpots with the diameter of 7cm and the depth of 7cm, and the seedlings are acclimatized in a control greenhouse in the next March. A new hybrid variety was generated as shown in FIG. 2 a.
Example 2
Hybrid breeding of linaria and baifeng
Taking lina lotus as a female parent (figure 1 a) and white phoenix as a male parent (figure 1 c), carrying out hybrid breeding, wherein the hybrid breeding is carried out in a greenhouse at the plant research institute of Chinese academy of sciences (Nanjing Zhongshan botanical garden) in Jiangsu province for 4 months and 1 day in 2020, the plant is selected to grow robustly and has no plant diseases and insect pests, a 4-5 year-old flowerbet is used for planting a seedling lina lotus ten pots, a 4-5 year-old flowerbet is used for cutting a seedling white phoenix, the seedling is bagged before the first flower is opened, the 16-month lina lotus and white phoenix lotus start to flower in 4 months, when 8-9 o 'lina lotus 33488is opened in the morning, the male parent white phoenix pollen is collected for pollination, 3-4 o's afternoon, and the second pollination is carried out on the lina lotus with the white phoenix pollen. The subsequent management was the same as in example 1. Two new hybrid varieties were generated as shown in FIGS. 2b and 2 c.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and amendments can be made without departing from the principle of the present invention, and these modifications and amendments should also be considered as the protection scope of the present invention.
Claims (10)
1. A method for interspecific hybridization of a new Lina lotus plant is characterized in that artificial interspecific hybridization is carried out by taking the Lina lotus of the genus Lycianthes as a female parent and taking the snow lotus or the white phoenix of the genus Lycianthes as a male parent to generate a new hybrid variety.
2. An interspecific cross breeding method according to claim 1, wherein the number ratio of female parent to male parent is 1.
3. An interspecies cross breeding method according to claim 1, wherein the artificial interspecies cross comprises: bagging is carried out before the first flower of the male parent and the female parent bloom, and when the first flower of the female parent blooms, pollen of the male parent is collected and pollinated for 2 times.
4. An interspecific cross-breeding method as claimed in claim 3, wherein the first pollination is performed at 8-9, and the second pollination is performed at 15-16.
5. The interspecific cross breeding method of claim 1, further comprising the steps of harvesting seeds of the new hybrid variety, sowing and managing the seedling.
6. An interspecific cross breeding method as claimed in claim 5, wherein the hybrid seeds are harvested and sown into a seedling raising substrate after they are matured.
7. An interspecific cross breeding method as claimed in claim 6, wherein the seedling raising substrate is divided into two layers, the thickness of the lower substrate is 5-8cm, peat: volcanic rock: vermiculite =8-12, 1-3, upper substrate particle size 0.3-0.7mm, thickness 0.4-0.6cm, fine peat: fine sand = 8-12.
8. An interspecific cross breeding method as claimed in claim 5 or 6, wherein after sowing, the temperature at night is controlled to 15-18 ℃ and the temperature during day is controlled to 22-25 ℃ and the substrate is kept wet.
9. An interspecies cross breeding method according to claim 5, wherein the management of growing seedlings comprises: after seedling emergence, spraying water twice a day, and covering a sunshade net in summer to keep ventilation.
10. An interspecific cross breeding method according to claim 9, wherein the spraying is performed once in the afternoon; the shading rate of the sunshade net is 65-75%; the ventilation volume is 2-3 levels.
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| CN202211227073.5A CN115399239A (en) | 2022-10-09 | 2022-10-09 | Interspecific cross breeding method for novel lina plant |
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| CN202211227073.5A CN115399239A (en) | 2022-10-09 | 2022-10-09 | Interspecific cross breeding method for novel lina plant |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180110168P1 (en) * | 2016-10-18 | 2018-04-19 | Renee O'Connell | SEDEVERIA x ECHEVERIA HYBRID PLANT NAMED 'LILAC MIST' |
| CN111066649A (en) * | 2020-01-16 | 2020-04-28 | 云南农业大学 | Sexual propagation method for echeveria succulent plants |
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- 2022-10-09 CN CN202211227073.5A patent/CN115399239A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180110168P1 (en) * | 2016-10-18 | 2018-04-19 | Renee O'Connell | SEDEVERIA x ECHEVERIA HYBRID PLANT NAMED 'LILAC MIST' |
| CN111066649A (en) * | 2020-01-16 | 2020-04-28 | 云南农业大学 | Sexual propagation method for echeveria succulent plants |
Non-Patent Citations (3)
| Title |
|---|
| 于红茹;张文新;: "拟石莲花属多肉植物生长特性及栽培繁殖要点", no. 16, pages 12 - 14 * |
| 师兄说电影: "多肉植物丽娜莲杂交培育出来的后代，看看各有多少不同" * |
| 童伟、王星: "实用盆栽花卉技术", 远方出版社, pages: 13 * |
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Inventor after: Gu Yonghua Inventor after: Chen Meixiang Inventor after: Quan Dazhi Inventor after: Huang Shengnan Inventor after: Ji Yang Inventor after: Song Zhengda Inventor before: Gu Yonghua Inventor before: Quan Dazhi Inventor before: Huang Shengnan Inventor before: Chen Meixiang Inventor before: Ji Yang Inventor before: Song Zhengda |
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