CN115053742B

CN115053742B - Seedling raising method for acer temple seeds

Info

Publication number: CN115053742B
Application number: CN202210797721.4A
Authority: CN
Inventors: 姜治国; 金胶胶; 余辉亮; 熊欢欢; 李友志
Original assignee: Shennongjia National Park Research Institute
Current assignee: Shennongjia National Park Research Institute
Priority date: 2022-07-08
Filing date: 2022-07-08
Publication date: 2024-03-12
Anticipated expiration: 2042-07-08
Also published as: CN115053742A

Abstract

The invention discloses a seedling raising method of acer temple seeds, which comprises the following steps: step one, sterilizing seeds, washing, and airing the seeds at a cool and ventilated place; digging a sand storage pit, uniformly mixing the dried seeds with wet river sand, filling the sand storage pit, paving a layer of wet river sand on the top, covering a woven bag, turning over once every 7-14 d, and spraying a proper amount of water until the seeds have bud points; step three, pouring a proper amount of carbendazim wettable powder on a seedbed, and then sowing seeds, wherein the seedbed comprises: the preparation method of the pine needle-clover mixture layer comprises the following steps of: soaking folium Pini, washing, drying, and cutting into folium Pini segments; pulverizing herba Trifolii Pratentis, soaking, filtering, and oven drying to obtain herba Trifolii Pratentis powder; mixing folium Pini segment with herba Trifolii Pratentis powder and hypromellose. The invention has the beneficial effect of improving the emergence rate of the acer temple seeds.

Description

Seedling raising method for acer temple seeds

Technical Field

The invention relates to the technical field of seed seedling raising. More particularly, the invention relates to a seedling raising method of acer temple seeds.

Background

The Acer Templianum is a plant of Acer of Sapindaceae, belongs to a national secondary important protection plant, has important economic value and ornamental value, but has small distribution range, difficult natural updating and optimistic survival condition. At present, few researches on artificial breeding of Acer temple are carried out, and the seed emergence rate is low. Therefore, how to design the seedling raising method of the acer above-mentioned seeds to obtain the improvement of the emergence rate of the acer above-mentioned seeds is worth.

Disclosure of Invention

It is an object of the present invention to solve at least the above problems and to provide at least the advantages to be described later.

To achieve these objects and other advantages and in accordance with the purpose of the invention, there is provided a seedling raising method of acer temple seeds, comprising the steps of:

step one, soaking seeds in a disinfectant for 8 to 15 minutes, washing the seeds with sterile water, and then airing the seeds in a cool and ventilated place;

digging a sand storage pit, uniformly mixing the dried seeds with wet river sand with the water content of 60-80% according to the volume ratio of 1:3-5, filling the seeds into the sand storage pit, paving a layer of thinner wet river sand on the top, covering a woven bag, turning once every 7-14 d, and spraying a proper amount of sterile water after each turning until bud points appear on 30-50% of the seeds;

step three, pouring a proper amount of 500-800 times of liquid medicine of 20-25 wt% carbendazim wettable powder on a seedbed, and then sowing seeds on the seedbed, wherein the seedbed comprises: a pine needle-clover mixture layer, a matrix soil layer and a perlite layer are sequentially paved from top to bottom;

the preparation method of the pine needle-clover mixture layer comprises the following steps:

soaking pine needles in soapy water for 1-2 h, washing, drying and cutting into 1-2 cm sections to obtain pine needle sections;

pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;

mixing pine needle segment, clover powder and hydroxypropyl methylcellulose in 2-5 wt% through stirring.

Preferably, the substrate soil layer in the third step comprises the following raw materials in parts by weight: 45-60 parts of brown soil, 30-40 parts of pine needles, 3-7 parts of compound microorganisms, 10-15 parts of sawmill residues and 2-6 parts of EM bacterial powder.

Preferably, the preparation method of the matrix soil layer in the third step comprises the following steps:

adding a proper amount of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;

soaking pine needles in soapy water for 1-2 h, cleaning, drying and crushing to obtain pine needle powder;

fermenting sawn timber residues by using compound microorganisms to obtain fermentation liquor and fermentation residues;

and (3) fully and uniformly stirring the brown soil mud, pine needle powder, fermentation liquor, fermentation residues and EM bacteria powder, regulating the pH value to 5.5-6.6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and covering the surface of the brown soil mud, pine needle powder, fermentation liquor, fermentation residues and EM bacteria powder with a degradable mulch film for 2-3 d to obtain the matrix soil layer.

Preferably, the disinfectant in the first step is a solution obtained by mixing 10-12 wt% of Boldo solution and 20-25 wt% of carbendazim according to a volume ratio of 1:1.

Preferably, the method also comprises the step of sterilizing the river sand in the step two, and comprises the following specific steps: the plastic cloth is wrapped and placed under the sun for 2 to 3 days after being sprayed by 0.2 to 0.4 weight percent potassium permanganate solution.

Preferably, the weight parts of the compound microorganism in the third step are 20-30 parts of propionic acid bacteria and 50-60 parts of lactobacillus acidophilus.

Preferably, the seedling bed in the third step has a ridge length of 1.2-1.5 m, a ridge width of 0.8-1 m, a ridge height of 0.15-0.25 m and a step width of 0.4-0.6 m.

Preferably, the sowing mode in the third step is drill sowing, and parameters of the drill sowing are as follows: the sowing width is 8-10 cm, the row spacing is 5-8 cm, and the depth is 4-6 cm.

The invention at least comprises the following beneficial effects:

according to the invention, the pine needle-clover mixture layer is used for covering a matrix soil layer, so that the moisture evaporation in the matrix soil layer is reduced, the temperature of the matrix soil layer is improved, the soil moisture preservation and moisture preservation effects are realized on the matrix soil layer, the emergence rate of temple chiffon seeds is improved, meanwhile, pine needles and clover are decomposed by microorganisms to form humus, and the humus provides nutrient components consisting of nutrient elements such as nitrogen, calcium and phosphorus for seedlings, and the growth of the seedlings is promoted.

Secondly, the research on the artificial breeding of the temple Acer is less at present, and the seed emergence rate is low.

Thirdly, fermenting sawn timber residues by using propionic acid bacteria and lactobacillus acidophilus to generate fermentation liquor and fermentation residues, wherein the fermentation liquor contains rich lactic acid and acetic acid, so that calcium, phosphorus and iron in soil and the fermentation residues are promoted to be converted into quick-acting calcium, quick-acting phosphorus and quick-acting iron which are easy to be absorbed by seedlings, and the fermentation residues are comprehensive in nutrition and do not cause plants to have nitrogen, phosphorus and potassium deficiency, so that a nutrient matrix of a matrix soil layer is improved; meanwhile, EM bacterial powder is matched, so that the diversity of bacterial colonies in the matrix soil layer is increased, the conversion and circulation of organic matters and nutrients in the matrix soil layer are promoted, and the balance of bacterial colonies in the matrix soil layer and nutrient matrixes is ensured.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Detailed Description

The present invention is described in further detail below with reference to examples to enable those skilled in the art to practice the same by referring to the description.

The experimental methods described in the following embodiments are conventional methods unless otherwise indicated, and the reagents and materials are commercially available.

Example 1 ]

The seedling raising method of the acer temple seeds comprises the following steps:

step one, soaking seeds in a disinfectant for 8min, washing with sterile water, and then airing in a cool and ventilated place;

digging a sand storage pit, uniformly mixing the dried seeds with wet river sand with the water content of 60% according to the volume ratio of 1:3, filling the seeds into the sand storage pit, paving a layer of thinner wet river sand on the top, covering a woven bag to preserve moisture and ventilate, turning every 7d, and spraying a proper amount of sterile water after each turning until 30% of the seeds have bud points;

step three, pouring 500 times of liquid medicine of 20wt% carbendazim wettable powder on a seedbed until water is discharged from the seedbed, and sowing seeds on the seedbed, wherein the seedbed comprises: a pine needle-clover mixture layer, a matrix soil layer and a perlite layer are sequentially paved from top to bottom;

soaking folium Pini in soapy water for 1 hr, washing, drying, and cutting into 1cm pieces to obtain folium Pini segments;

the pine needle segments and clover powder, 2.6g of 2wt% hydroxypropyl methylcellulose are stirred and mixed uniformly.

The matrix soil layer in the third step comprises the following raw materials in parts by weight: 45 parts of brown soil, 30 parts of pine needles, 3 parts of compound microorganisms, 10 parts of sawmill residues and 2 parts of EM bacteria powder.

The preparation method of the matrix soil layer comprises the following steps:

adding 450mL of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;

soaking folium Pini in soapy water for 1 hr, cleaning, drying, and pulverizing to obtain folium Pini powder;

and (3) fully and uniformly stirring the brown soil mud, pine needle powder, fermentation liquor, fermentation residues and EM bacteria powder, regulating the pH value to 5.5 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and covering the surface of the brown soil mud, pine needle powder, fermentation liquor, fermentation residues and EM bacteria powder with a degradable mulch film 2d to obtain the matrix soil layer.

The disinfectant in the first step is a solution obtained by mixing 10wt% of Boldo solution and 20wt% of carbendazim according to a volume ratio of 1:1.

The method also comprises the step of sterilizing river sand in the step two, and specifically comprises the following steps: after being sprayed with 0.2wt% potassium permanganate solution, the plastic cloth is wrapped and is sun-dried for 2 days.

The weight parts of the compound microorganism in the third step are 20 parts of propionic acid bacteria and 50 parts of lactobacillus acidophilus.

The length of the ridge of the seedbed in the third step is 1.2m, the width of the ridge is 0.8m, the height of the ridge is 0.15m, and the width of the pavement is 0.4m.

The sowing mode in the third step is drill sowing, and parameters of the drill sowing are as follows: the sowing width is 8cm, the row spacing is 5cm, and the depth is 4cm.

Example 2 ]

step one, soaking seeds in a disinfectant for 10min, washing with sterile water, and then airing in a cool and ventilated place;

digging a sand storage pit, uniformly mixing the dried seeds with wet river sand with the water content of 70% according to the volume ratio of 1:4, filling the seeds into the sand storage pit, paving a layer of thinner wet river sand on the top, covering a woven bag to preserve moisture and ventilate, turning every 10d, and spraying a proper amount of sterile water after turning every time until 40% of the seeds have bud points;

step three, pouring 650 times of liquid medicine of 23wt% carbendazim wettable powder on a seedbed until water is discharged from the seedbed, and sowing seeds on the seedbed, wherein the seedbed comprises: a pine needle-clover mixture layer, a matrix soil layer and a perlite layer are sequentially paved from top to bottom;

soaking folium Pini in soapy water for 1.5 hr, washing, drying, and cutting into 1.5cm pieces to obtain folium Pini segments;

the pine needle segments and clover powder, 12g of 3wt percent hydroxypropyl methylcellulose are stirred and mixed uniformly.

The matrix soil layer in the third step comprises the following raw materials in parts by weight: 53 parts of brown soil, 35 parts of pine needles, 5 parts of compound microorganisms, 13 parts of sawmill residues and 4 parts of EM bacterial powder.

The preparation method of the matrix soil layer comprises the following steps:

adding 530mL of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;

soaking folium Pini in soapy water for 1.5 hr, cleaning, drying, and pulverizing to obtain folium Pini powder;

and (3) fully and uniformly stirring the brown soil mud, pine needle powder, fermentation liquor, fermentation residues and EM bacteria powder, regulating the pH value to 6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and covering the surface of the brown soil mud, pine needle powder, fermentation liquor, fermentation residues and EM bacteria powder with a degradable mulch film for 2.5d to obtain the matrix soil layer.

The disinfectant in the first step is a solution obtained by mixing 11wt% of Boldo solution and 23wt% of carbendazim according to a volume ratio of 1:1.

The method also comprises the step of sterilizing river sand in the step two, and specifically comprises the following steps: after being sprayed with 0.3wt% potassium permanganate solution, the plastic cloth is wrapped and is sun-dried for 2d.

The weight parts of the compound microorganism in the third step are 25 parts of propionic acid bacteria and 55 parts of lactobacillus acidophilus.

The length of the ridge of the seedbed in the third step is 1.3m, the width of the ridge is 0.9m, the height of the ridge is 0.2m, and the width of the pavement is 0.5m.

The sowing mode in the third step is drill sowing, and parameters of the drill sowing are as follows: the sowing width is 9cm, the row spacing is 7cm, and the depth is 5cm.

Example 3 ]

step one, soaking seeds in a disinfectant for 15min, washing with sterile water, and then airing in a cool and ventilated place;

digging a sand storage pit, uniformly mixing the dried seeds with wet river sand with the water content of 80% according to the volume ratio of 1:5, filling the seeds into the sand storage pit, paving a layer of thinner wet river sand on the top, covering a woven bag to preserve moisture and ventilate, turning every 14d, and spraying a proper amount of sterile water after each turning until 50% of the seeds have bud points;

step three, pouring 800 times of liquid medicine of 25wt% carbendazim wettable powder on a seedbed until water is discharged from the seedbed, and sowing seeds on the seedbed, wherein the seedbed comprises: a pine needle-clover mixture layer, a matrix soil layer and a perlite layer are sequentially paved from top to bottom;

soaking folium Pini in soapy water for 2 hr, washing, drying, and cutting into 2cm pieces to obtain folium Pini segments;

the pine needle segments and clover powder, 25g of 5wt% hydroxypropyl methylcellulose are stirred and mixed uniformly.

The matrix soil layer in the third step comprises the following raw materials in parts by weight: 60 parts of brown soil, 40 parts of pine needles, 7 parts of compound microorganisms, 15 parts of sawmill residues and 6 parts of EM bacterial powder.

The preparation method of the matrix soil layer comprises the following steps:

adding 600mL of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;

soaking folium Pini in soapy water for 2 hr, cleaning, drying, and pulverizing to obtain folium Pini powder;

and (3) fully and uniformly stirring the brown soil mud, pine needle powder, fermentation liquor, fermentation residues and EM bacteria powder, regulating the pH value to 6.6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and covering the surface of the brown soil mud, pine needle powder, fermentation liquor, fermentation residues and EM bacteria powder with a degradable mulch film for 3d to obtain the matrix soil layer.

The disinfectant in the first step is a solution obtained by mixing 12wt% of Boldo solution and 25wt% of carbendazim according to a volume ratio of 1:1.

The method also comprises the step of sterilizing river sand in the step two, and specifically comprises the following steps: after being sprayed with 0.4wt% potassium permanganate solution, the plastic cloth is wrapped and dried in the sun for 3d.

The weight parts of the compound microorganism in the third step are 30 parts of propionic acid bacteria and 60 parts of lactobacillus acidophilus.

The length of the ridge of the seedbed in the third step is 1.5m, the width of the ridge is 1m, the height of the ridge is 0.25m, and the width of the walk is 0.6m.

The sowing mode in the third step is drill sowing, and parameters of the drill sowing are as follows: the sowing width is 10cm, the row spacing is 8cm, and the depth is 6cm.

< blank group >

Step one, placing seeds in a sterilizing solution obtained by mixing 12wt% of Barduo solution and 25wt% of carbendazim according to the volume ratio of 1:1, soaking for 15min, washing with sterile water, and then placing in a cool and ventilated place for airing;

digging a sand storage pit, wrapping plastic cloth sprayed by 0.4wt% potassium permanganate solution in the sun for 3d disinfection, flushing with sterile water, uniformly mixing wet river sand with the water content of 80% and the dried seeds according to the volume ratio of 1:5, filling the mixture into the sand storage pit, paving a layer of thinner wet river sand on the top, covering a woven bag on the top, preserving moisture and ventilating, turning once every 14d, and spraying a proper amount of sterile water after each turning until 50% of the seeds have bud points;

step three, pouring 800 times of liquid medicine of 25wt% carbendazim wettable powder before sowing until water is discharged from the seedbed, and sowing seeds on a sandy loam seedbed with a ridge length of 1.5m, a ridge width of 1m, a ridge height of 0.25m and a walk width of 0.6m in a drill sowing mode with a sowing width of 10cm, a row spacing of 8cm and a depth of 6cm.

< measurement experiments >

The emergence rate, the seedling height after 1 month of emergence, and the root length were measured after sowing in each of the examples and the blank groups, and the results are shown in table 1:

TABLE 1 seedling emergence rate after sowing Acer Templianum seeds, seedling height and root length after 1 month of emergence

	Emergence rate%	Seedling height (cm)	Root length (cm)
				Blank group	35.00	6.13	4.08
Example 1	41.00	6.65	4.13
				Example 2	44.00	6.68	4.26
Example 3	52.00	7.83	4.63

As can be seen from Table 1, the seedling height and root length of the acer temple seeds cultivated by the methods of examples 1-3 are superior to those of the blank group, wherein the seedling emergence rate of the acer temple seeds cultivated by example 3 after sowing reaches 52%, the seedling height of the acer temple seeds after 1 month of emergence reaches 7.83cm, and the root length of the acer temple seeds after 1 month of emergence reaches 4.63cm, thereby laying a foundation for the seedling alignment and strengthening of the subsequent acer temple seedlings.

Comparative example 1 ]

digging a sand storage pit, namely wrapping plastic cloth which is sprayed by 0.4wt% of potassium permanganate solution and then sun-dried for 3 days, flushing with sterile water, uniformly mixing wet river sand with the water content of 80% with the dried seeds according to the volume ratio of 1:5, filling the mixture into the sand storage pit, paving a layer of thinner wet river sand on the top, covering a woven bag on the top, preserving moisture and ventilation, turning every 14 days, and spraying a proper amount of sterile water after each turning until 50% of the seeds have bud points;

step three, sowing on a seedling bed with a ridge length of 1.5m, a ridge width of 1m, a ridge height of 0.25m and a walk width of 0.6m in a drill sowing mode with a sowing width of 10cm, a row spacing of 8cm and a depth of 6cm, applying 800 times of liquid medicine of 25wt% carbendazim wettable powder until water in the seedling bed is discharged, and sowing seeds on the seedling bed, wherein the seedling bed comprises: a covering layer (formed by single or synergistic combination of pine needle segments, clover powder and hypromellose), a matrix soil layer and a perlite layer which are sequentially paved from top to bottom;

the cover layers are respectively arranged as follows:

a: a pine needle segment; b: clover powder; c: hydroxypropyl methylcellulose; d: pine needle segment + clover powder; e: pine needle segment, clover powder and hydroxypropyl methylcellulose.

The processing method of the covering layer comprises the following steps:

when the cover layer is A, soaking the pine needle in soapy water for 2 hours, and then washing, drying and cutting into 2cm to obtain a pine needle section;

when the cover layer is B, pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;

when the cover layer is C, 25g of 5wt% hydroxypropyl methylcellulose is directly added;

when the covering layer is D, stirring and mixing the A and the B uniformly;

when the cover layer is E, A, B, C is stirred and mixed uniformly.

The matrix soil layer in the third step comprises the following raw materials in parts by weight: 60 parts of brown soil, 40 parts of pine needles, 7 parts of compound microorganisms (30 parts of propionic acid bacteria and 60 parts of lactobacillus acidophilus are mixed), 15 parts of saw dust and 6 parts of EM bacteria powder.

The preparation method of the matrix soil layer comprises the following steps:

The emergence rate of the blank group and the comparative example 1 after sowing was counted, and the results are shown in the following table 2:

table 2 emergence rate of acer ginnala seeds after sowing

As shown in table 2, the emergence rate of the acer above-mentioned seeds grown by the method of the blank group was 35%, which is relatively low, and the emergence rate of the acer above-mentioned seeds grown by the seedbed formed by the cover layer A, B, C, D was similar to that of the blank group, and the cover layer A, B, C, D did not promote the emergence rate positively. The emergence rate of the temple seeds cultivated by the cover layer E reaches 52%, which shows that the cover layer E has positive promotion effect on the emergence rate, and the analysis reasons are that the water retention effect of the cover layer is greatly increased under the effect of hydroxypropyl methylcellulose by pine needle sections and clover powder, and the moisture preservation effect is realized on the underlying matrix soil layer, so that the emergence rate of temple chives seeds is improved.

Comparative example 2 ]

step three, sowing on a seedling bed with a ridge length of 1.5m, a ridge width of 1m, a ridge height of 0.25m and a walk width of 0.6m in a drill sowing mode with a sowing width of 10cm, a row spacing of 8cm and a depth of 6cm, applying 800 times of liquid medicine of 25wt% carbendazim wettable powder until water in the seedling bed is discharged, and sowing seeds on the seedling bed, wherein the seedling bed comprises: a covering layer (respectively containing original pine needles, 5cm pine needle sections, 2cm pine needle sections and pine needle powder), a matrix soil layer and a perlite layer which are sequentially paved from top to bottom;

the processing method of the covering layer comprises the following steps:

soaking folium Pini in soapy water for 2 hr, washing, drying, and respectively processing to obtain original folium Pini, 5cm folium Pini segment, 2cm folium Pini segment, and folium Pini powder;

the original pine needles, 5cm pine needle sections, 2cm pine needle sections, pine needle powder, clover powder and 25g 5wt% hydroxypropyl methylcellulose are respectively stirred and mixed uniformly to form covering layers in the seedbeds of the group 1, the group 2, the group 3 and the group 4.

The preparation method of the matrix soil layer comprises the following steps:

The daily temperature differences of the beds of the blank and comparative example 2 at soil layers of different depths were measured and the results are shown in table 3 below:

TABLE 3 temperature differences of seedbeds over different depths of soil

	Blank group	Group 1	Group 2	Group 3	Group 4
						Surface of the body	23.7℃	22.5℃	23.2℃	22.2℃	23.1℃
10cm	13.0℃	12℃	11.9℃	9.2℃	12.3℃
						20cm	7.2℃	6.8℃	6.9℃	6.7℃	7.0℃

As can be seen from table 3, the four seedling beds in the blank group and the comparative example 2 have no significant difference in daily temperature difference between the 20cm soil layer, and the difference in daily temperature difference between the surface and the 10cm soil layer is shown, wherein the group 3 seedling bed with the 2cm pine needle section as the cover layer has the smallest daily temperature difference between the 10cm soil layer, and compared with the blank seedling bed, the daily temperature difference is reduced by 4.8 ℃, the analysis reasons are that the 2cm pine needle section effectively controls the evaporation rate of water in the seedling bed, adjusts the temperature difference in the soil, is beneficial to respiration of microorganisms, decomposes mineral nutrient elements in the soil, and provides rich nutrient components for the Acer samaratus seeds.

Comparative example 3 ]

digging a sand storage pit, namely wrapping the dried seeds with plastic cloth sprayed by 0.4wt% of potassium permanganate solution, airing for 3d under the sun, flushing with sterile water, uniformly mixing wet river sand with the water content of 80% according to the volume ratio of 1:5, filling the mixture into the sand storage pit, paving a layer of wet river sand on the top, turning once every 14d, and spraying a proper amount of sterile water after each turning until 50% of the seeds have bud points;

step three, sowing on a seedling bed with a ridge length of 1.5m, a ridge width of 1m, a ridge height of 0.25m and a walk width of 0.6m in a drill sowing mode with a sowing width of 10cm, a row spacing of 8cm and a depth of 6cm, applying 800 times of liquid medicine of 25wt% carbendazim wettable powder until water in the seedling bed is discharged, and sowing seeds on the seedling bed, wherein the seedling bed comprises: a pine needle-clover mixture layer, a nutrient soil layer and a perlite layer are sequentially paved from top to bottom;

The nutrient soil layer in the third step is prepared by adding the following raw materials in parts by weight on the basis of 6 parts of EM bacterial powder:

group a:100 parts of brown soil; group b:78 parts of brown soil, 7 parts of compound microorganisms and 15 parts of sawn timber residues; group c:85 parts of brown soil plus 15 parts of pine needles; group d:60 parts of brown soil, 7 parts of compound microorganisms, 40 parts of pine needles and 15 parts of sawn timber residues;

wherein, the weight parts of the compound microorganism are 30 parts of propionic acid bacteria and 60 parts of lactobacillus acidophilus.

The preparation method of the nutrient soil layer comprises the following steps:

and (3) fully and uniformly stirring the group a, the group b, the group c, the group d and the EM bacteria powder, regulating the pH value to 6.6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and then covering the surfaces of the groups with degradable mulch films 3d to obtain each group of nutrient soil layers.

The available nutrient content of the blank and comparative example 4 seedbeds at 10cm soil layer was measured and the results are shown in Table 4:

TABLE 4 available nutrient content of seedbed in 10cm soil layer

	Blank group	Group a	Group b	Group c	Group d
						Quick-acting nitrogen (mg/kg)	170.82	200.17	237.79	213.56	473.41
Quick-acting phosphorus (mg/kg)	7.85	11.21	15.46	10.97	20.25
						Quick-acting calcium (mg/kg)	140.31	315.45	400.52	328.69	450.06
Quick-acting iron (mg/kg)	31.86	33.25	40.58	36.81	62.41

As can be seen from Table 4, the nutrient contents of quick-acting nitrogen, quick-acting phosphorus, quick-acting calcium and quick-acting iron in the nutrient soil layer of the group d are higher than those of the blank group, the group a, the group b and the group c, and the analysis reasons are probably that humus is formed after pine needles are degraded, fermentation liquor generated by fermenting saw wood residues under the action of compound microorganisms promotes the conversion of humus and calcium, phosphorus and iron in the fermentation residues into seedlings to absorb quick-acting nitrogen, quick-acting phosphorus and quick-acting calcium, and mineral nutrient elements such as quick-acting iron and the like which are rich in brown soil are matched, so that the quick-acting nutrients of the nutrient soil layer are greatly increased, and balanced nutrients are provided for the growth of subsequent maple seedlings of the temple.

Although embodiments of the present invention have been disclosed above, it is not limited to the details and embodiments shown, it is well suited to various fields of use for which the invention is suited, and further modifications may be readily made by one skilled in the art, and the invention is therefore not to be limited to the particular details and examples shown and described herein, without departing from the general concepts defined by the claims and the equivalents thereof.

Claims

1. The seedling raising method for the acer temple seeds is characterized by comprising the following steps of:

digging a sand storage pit, uniformly mixing the dried seeds with wet river sand with the water content of 60-80% according to the volume ratio of 1:3-5, filling the seeds into the sand storage pit, paving a layer of thinner wet river sand on the top, covering a woven bag, turning once every 7-14 d, and spraying a proper amount of sterile water after each turning until 30-50% of the seeds have bud points;

uniformly stirring and mixing pine needle segments, clover powder and 2-5 wt% of hydroxypropyl methylcellulose;

the matrix soil layer in the third step comprises the following raw materials in parts by weight: 45-60 parts of brown soil, 30-40 parts of pine needles, 3-7 parts of compound microorganisms, 10-15 parts of sawmill residues and 2-6 parts of EM bacterial powder;

the preparation method of the matrix soil layer comprises the following steps:

2. The method for raising the seedlings of the acer temple seeds according to claim 1, wherein the disinfectant in the first step is a solution obtained by mixing 10-12 wt% of Boldo solution and 20-25 wt% of carbendazim according to a volume ratio of 1:1.

3. The method for raising the seedlings of the acer temple seeds according to claim 1, further comprising the step of sterilizing river sand in the second step, and specifically comprising the following steps: the plastic cloth is wrapped and placed under the sun for 2 to 3 days after being sprayed by 0.2 to 0.4 weight percent potassium permanganate solution.

4. The method for raising seedlings of acer temple seeds according to claim 1, wherein the compound microorganism in the third step is 20-30 parts by weight of propionic acid bacteria and 50-60 parts by weight of lactobacillus acidophilus.

5. The method for raising the seedlings of the acer temple seeds according to claim 1, wherein the ridge length of the seedbed in the third step is 1.2-1.5 m, the ridge width is 0.8-1 m, the ridge height is 0.15-0.25 m, and the pavement width is 0.4-0.6 m.

6. The method for raising maple seeds according to claim 1, wherein the seeding mode in the third step is drill seeding, and drill seeding parameters are: the sowing width is 8-10 cm, the row spacing is 5-8 cm, and the depth is 4-6 cm.