CN115053742A - Seedling growing method for temple acer mono seeds - Google Patents

Seedling growing method for temple acer mono seeds Download PDF

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CN115053742A
CN115053742A CN202210797721.4A CN202210797721A CN115053742A CN 115053742 A CN115053742 A CN 115053742A CN 202210797721 A CN202210797721 A CN 202210797721A CN 115053742 A CN115053742 A CN 115053742A
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seeds
powder
parts
layer
temple
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CN115053742B (en
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姜治国
金胶胶
余辉亮
熊欢欢
李友志
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Shennongjia National Park Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • A01G13/02Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
    • A01G13/0256Ground coverings
    • A01G13/0262Mulches, i.e. covering material not-pre-formed in mats or sheets
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/23Wood, e.g. wood chips or sawdust
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/40Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
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  • Inorganic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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Abstract

The invention discloses a seedling growing method of temple acer mono seeds, which comprises the following steps: step one, sterilizing and washing seeds, and airing the seeds in a cool and ventilated place; digging a sand storage pit, uniformly mixing the dried seeds with wet river sand, filling the mixture into the sand storage pit, laying a layer of wet river sand on the top, covering a woven bag, turning once every 7-14 days, and spraying a proper amount of water until the seeds have bud points; step three, pouring a proper amount of carbendazim wettable powder on a seedbed, and then sowing seeds, wherein the seedbed comprises: the pine needle-clover mixture layer, the matrix soil layer and the perlite layer are sequentially paved from top to bottom, wherein the preparation method of the pine needle-clover mixture layer comprises the following steps: soaking folium Pini, washing, drying, and cutting into segments; pulverizing herba Trifolii Pratentis, soaking, filtering, and oven drying to obtain herba Trifolii Pratentis powder; mixing folium Pini segment, herba Trifolii Pratentis powder, and hypromellose. The invention has the beneficial effect of improving the emergence rate of the temple acer mono seeds.

Description

Seedling growing method for temple acer mono seeds
Technical Field
The invention relates to the technical field of seed seedling culture. More particularly, the invention relates to a seedling growing method of temple acer mono seeds.
Background
The Temple acer truncatum is a plant of the genus Acer of the family Sapindaceae, belongs to a national secondary key protection plant, has important economic value and ornamental value, but has small distribution range, difficult natural renewal and unobjective survival condition. At present, few researches on artificial breeding of the Temple maple are carried out, and the rate of emergence of seeds is low. Therefore, how to design the seedling raising method of the Temple maple seeds to improve the emergence rate of the Temple maple seeds is worthy of deep thought.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for growing seedlings of temple acer mono seeds, comprising the steps of:
soaking seeds in a disinfectant for 8-15 min, washing with sterile water, and then placing in a cool and ventilated place for airing;
step two, digging a sand storage pit, uniformly mixing the aired seeds with wet river sand with the water content of 60-80% according to the volume ratio of 1: 3-5, filling the mixture into the sand storage pit, laying a layer of thin wet river sand on the top, covering a woven bag, turning the mixture once every 7-14 days, and spraying a proper amount of sterile water after each turning until 30-50% of the seeds have bud spots;
step three, pouring a proper amount of 20-25 wt% carbendazim wettable powder 500-800 times of liquid medicine on a seedbed, and then sowing seeds on the seedbed, wherein the seedbed comprises: sequentially paving a pine needle-clover mixture layer, a matrix soil layer and a perlite layer from top to bottom;
the preparation method of the pine needle-clover mixture layer comprises the following steps:
soaking pine needles in soap water for 1-2 h, then washing, drying and cutting into 1-2 cm to obtain pine needle sections;
pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;
and stirring and mixing the pine needle segments, the clover powder and 2-5 wt% of hydroxypropyl methylcellulose uniformly.
Preferably, the matrix soil layer in the third step comprises the following raw materials in parts by weight: 45-60 parts of brown soil, 30-40 parts of pine needles, 3-7 parts of compound microorganisms, 10-15 parts of sawmilling residues and 2-6 parts of EM (effective microorganism) powder.
Preferably, the preparation method of the matrix soil layer in the third step comprises the following steps:
adding a proper amount of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;
soaking pine needles in soap water for 1-2 hours, cleaning, drying and crushing to obtain pine needle powder;
fermenting the sawn wood residues by using compound microorganisms to obtain a fermentation liquid and fermentation residues;
fully and uniformly stirring the brown soil mud, the pine needle powder, the fermentation liquor, the fermentation residues and the EM bacteria powder, adjusting the pH to 5.5-6.6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and then covering a degradable mulching film on the surface of the soil for 2-3 days to obtain the matrix soil layer.
Preferably, the disinfectant in the first step is a solution prepared by mixing 10-12 wt% of Bordeaux mixture and 20-25 wt% of carbendazim according to the volume ratio of 1: 1.
Preferably, the method further comprises the step two of disinfecting the river sand, and the specific method comprises the following steps: and (3) spraying 0.2-0.4 wt% of potassium permanganate solution, wrapping the plastic cloth, and airing the plastic cloth in the sun for 2-3 days.
Preferably, the weight parts of the compound microorganism in the third step are 20-30 parts of propionic acid bacteria and 50-60 parts of lactobacillus acidophilus.
Preferably, the seedbed in the third step has a ridge length of 1.2-1.5 m, a ridge width of 0.8-1 m, a ridge height of 0.15-0.25 m and a step width of 0.4-0.6 m.
Preferably, the sowing mode of the step three is drill sowing, and the parameters of the drill sowing are as follows: the sowing width is 8-10 cm, the row spacing is 5-8 cm, and the depth is 4-6 cm.
The invention at least comprises the following beneficial effects:
firstly, the pine needle-clover mixture layer is adopted to cover the matrix soil layer, so that the water evaporation in the matrix soil layer is reduced, the temperature of the matrix soil layer is increased, the soil moisture preservation and moisturizing effects are achieved on the matrix soil layer, the emergence rate of the Temple chiffon seeds is improved, meanwhile, the pine needle and the clover are decomposed by microorganisms to form humus, and the humus provides the seedlings with nutrient components consisting of nutrient elements such as nitrogen, calcium, phosphorus and the like, so that the growth of the seedlings is promoted.
Secondly, at present, less research on artificial breeding of temple acer mono is carried out, and the emergence rate of seeds is low.
Thirdly, fermenting the sawmilling residues by adopting propionic acid bacteria and lactobacillus acidophilus to generate a fermentation liquid and fermentation residues, wherein the fermentation liquid contains abundant lactic acid and acetic acid, and promotes calcium, phosphorus and iron in soil and the fermentation residues to be converted into quick-acting calcium, quick-acting phosphorus and quick-acting iron which are easy to absorb by seedlings, and the fermentation residues are comprehensive in nutrition and cannot cause the plants to have rich nitrogen and lack phosphorus and potassium, so that the nutrient substrate of a substrate soil layer is enhanced; meanwhile, EM bacterial powder is matched, the diversity of bacterial colonies in a matrix soil layer is increased, the conversion and circulation of organic matters and nutrients in the matrix soil layer are promoted, and the balance of the bacterial colonies and the nutrient medium in the matrix soil layer is guaranteed.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.
< example 1>
The seedling raising method of temple acer mono seeds comprises the following steps:
soaking seeds in a disinfectant for 8min, washing with sterile water, and then placing in a cool and ventilated place for airing;
step two, digging a sand storage pit, uniformly mixing the aired seeds with wet river sand with the water content of 60% according to the volume ratio of 1:3, filling the mixture into the sand storage pit, laying a layer of thinner wet river sand on the top of the sand storage pit, covering a woven bag to preserve moisture and ventilate, turning the sand storage pit once every 7 days, and spraying a proper amount of sterile water after each turning until 30% of the seeds have bud spots;
step three, pouring 500 times of liquid medicine of 20 wt% carbendazim wettable powder on a seedbed until the seedbed is drained, and then sowing seeds on the seedbed, wherein the seedbed comprises: sequentially paving a pine needle-clover mixture layer, a matrix soil layer and a perlite layer from top to bottom;
the preparation method of the pine needle-clover mixture layer comprises the following steps:
soaking folium Pini in soap water for 1 hr, washing, drying, and cutting into 1cm to obtain folium Pini segments;
pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;
stirring and mixing the pine needle segments, the clover powder and 2.6g of 2 wt% hydroxypropyl methylcellulose uniformly.
The matrix soil layer in the third step comprises the following raw materials in parts by weight: 45 parts of brown soil, 30 parts of pine needles, 3 parts of compound microorganisms, 10 parts of sawmilling residues and 2 parts of EM (effective microorganism) powder.
The preparation method of the matrix soil layer in the third step comprises the following steps:
adding 450mL of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;
soaking folium Pini in soap water for 1 hr, cleaning, drying, and pulverizing to obtain folium Pini powder;
fermenting the sawn wood residues by using compound microorganisms to obtain a fermentation liquid and fermentation residues;
fully and uniformly stirring the brown soil mud, the pine needle powder, the fermentation liquor, the fermentation residues and the EM bacteria powder, adjusting the pH to 5.5 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and then covering a degradable mulching film for 2d on the surface of the soil to obtain the matrix soil layer.
The disinfectant in the first step is a solution obtained by mixing 10 wt% of Bordeaux mixture and 20 wt% of carbendazim according to the volume ratio of 1: 1.
And the method also comprises the step two of disinfecting river sand, and the specific method comprises the following steps: and (3) spraying a 0.2 wt% potassium permanganate solution, wrapping with a plastic cloth, and drying in the sun for 2 d.
And the third step, wherein the compound microorganism comprises 20 parts of propionic acid bacteria and 50 parts of lactobacillus acidophilus by weight.
And the seedbed in the third step has the ridge length of 1.2m, the ridge width of 0.8m, the ridge height of 0.15m and the step width of 0.4 m.
The sowing mode of the step three is drill sowing, and the parameters of the drill sowing are as follows: the seeding width is 8cm, the row spacing is 5cm, and the depth is 4 cm.
< example 2>
The seedling raising method of the temple acer mono seeds comprises the following steps:
soaking seeds in a disinfectant for 10min, washing with sterile water, and then placing in a cool and ventilated place for airing;
step two, digging a sand storage pit, uniformly mixing the aired seeds with wet river sand with the water content of 70% according to the volume ratio of 1:4, filling the mixture into the sand storage pit, laying a layer of thinner wet river sand on the top, covering a woven bag to keep moisture and ventilate, turning the mixture once every 10 days, and spraying a proper amount of sterile water after each turning until 40% of the seeds have bud spots;
step three, pouring 650 times of liquid medicine of 23 wt% carbendazim wettable powder on a seedbed until the seedbed is drained, and then sowing seeds on the seedbed, wherein the seedbed comprises: sequentially paving a pine needle-clover mixture layer, a matrix soil layer and a perlite layer from top to bottom;
the preparation method of the pine needle-clover mixture layer comprises the following steps:
soaking folium Pini in soap water for 1.5h, washing, drying, and cutting into 1.5cm to obtain folium Pini segments;
pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;
stirring and mixing the pine needle segments, the clover powder and 12g of 3 wt% hydroxypropyl methylcellulose uniformly.
The matrix soil layer in the third step comprises the following raw materials in parts by weight: 53 parts of brown soil, 35 parts of pine needles, 5 parts of compound microorganisms, 13 parts of sawmilling residues and 4 parts of EM (effective microorganism) powder.
The preparation method of the matrix soil layer in the third step comprises the following steps:
adding 530mL of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;
soaking folium Pini in soap water for 1.5h, cleaning, drying, and pulverizing to obtain folium Pini powder;
fermenting the sawn wood residues by using compound microorganisms to obtain a fermentation liquid and fermentation residues;
fully and uniformly stirring the brown soil mud, the pine needle powder, the fermentation liquor, the fermentation residues and the EM bacteria powder, adjusting the pH to 6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and then covering a degradable mulching film on the surface of the soil for 2.5 days to obtain the matrix soil layer.
The disinfectant in the first step is a solution obtained by mixing 11 wt% of Bordeaux mixture and 23 wt% of carbendazim according to the volume ratio of 1: 1.
And the method also comprises the step two of disinfecting river sand, and the specific method comprises the following steps: and (3) spraying a 0.3 wt% potassium permanganate solution, wrapping with a plastic cloth, and drying in the sun for 2 d.
And the third step is that the composite microorganism accounts for 25 parts of propionic acid bacteria and 55 parts of lactobacillus acidophilus by weight.
And the seedbed in the third step has the ridge length of 1.3m, the ridge width of 0.9m, the ridge height of 0.2m and the step width of 0.5 m.
The sowing mode of the step three is drill sowing, and the parameters of the drill sowing are as follows: the seeding width is 9cm, the row spacing is 7cm, and the depth is 5 cm.
< example 3>
The seedling raising method of temple acer mono seeds comprises the following steps:
soaking seeds in a disinfectant for 15min, washing with sterile water, and then placing in a cool and ventilated place for airing;
step two, digging a sand storage pit, uniformly mixing the aired seeds with wet river sand with the water content of 80% according to the volume ratio of 1:5, filling the mixture into the sand storage pit, laying a layer of thinner wet river sand on the top, covering a woven bag to keep moisture and ventilate, turning the mixture once every 14 days, and spraying a proper amount of sterile water after each turning until 50% of the seeds have bud spots;
step three, pouring 800 times of liquid medicine of 25 wt% carbendazim wettable powder on a seedbed until the seedbed is drained, and then sowing seeds on the seedbed, wherein the seedbed comprises: sequentially paving a pine needle-clover mixture layer, a matrix soil layer and a perlite layer from top to bottom;
the preparation method of the pine needle-clover mixture layer comprises the following steps:
soaking folium Pini in soap water for 2 hr, washing, drying, and cutting into 2cm segments;
pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;
stirring and mixing the pine needle segments, the clover powder and 25g of 5 wt% hydroxypropyl methylcellulose uniformly.
The matrix soil layer in the third step comprises the following raw materials in parts by weight: 60 parts of brown soil, 40 parts of pine needles, 7 parts of compound microorganisms, 15 parts of sawn wood residues and 6 parts of EM (effective microorganism) powder.
The preparation method of the matrix soil layer in the third step comprises the following steps:
adding 600mL of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;
soaking folium Pini in soap water for 2 hr, cleaning, drying, and pulverizing to obtain folium Pini powder;
fermenting the sawn wood residues by using compound microorganisms to obtain a fermentation liquid and fermentation residues;
fully and uniformly stirring the brown soil mud, the pine needle powder, the fermentation liquor, the fermentation residues and the EM bacteria powder, adjusting the pH to 6.6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and then covering a degradable mulching film for 3 days on the surface of the soil to obtain the matrix soil layer.
The disinfectant in the first step is a solution obtained by mixing 12 wt% of Bordeaux mixture and 25 wt% of carbendazim according to the volume ratio of 1: 1.
And the method also comprises the step two of disinfecting river sand, and the specific method comprises the following steps: and (3) spraying a 0.4 wt% potassium permanganate solution, wrapping with a plastic cloth, and drying in the sun for 3 d.
And the third step is that the composite microorganism accounts for 30 parts of propionic acid bacteria and 60 parts of lactobacillus acidophilus by weight.
And the seedbed in the third step has the ridge length of 1.5m, the ridge width of 1m, the ridge height of 0.25m and the step width of 0.6 m.
The sowing mode of the step three is drill sowing, and the parameters of the drill sowing are as follows: the seeding width is 10cm, the row spacing is 8cm, and the depth is 6 cm.
< blank group >
Firstly, soaking seeds in disinfectant obtained by mixing 12 wt% of Bordeaux mixture and 25 wt% of carbendazim according to the volume ratio of 1:1 for 15min, washing with sterile water, and then airing in a cool and ventilated place;
digging a sand storage pit, wrapping plastic cloth which is sprayed with 0.4 wt% potassium permanganate solution, placing the plastic cloth in the sun, drying the plastic cloth in the sun for 3d sterilization, washing the plastic cloth with sterile water, uniformly mixing wet river sand with the water content of 80% and the dried seeds according to the volume ratio of 1:5, filling the mixture into the sand storage pit, laying a layer of thinner wet river sand on the top, covering a woven bag, keeping moisture and ventilating, turning the mixture once every 14d, and spraying a proper amount of sterile water after each turning until 50% of the seeds have bud spots;
and thirdly, pouring 800 times of liquid medicine of 25 wt% carbendazim wettable powder before sowing until the seedbed is drained, and sowing seeds on the sandy loam seedbed with the ridge length of 1.5m, the ridge width of 1m, the ridge height of 0.25m and the step width of 0.6m in a drilling mode with the sowing width of 10cm, the row spacing of 8cm and the depth of 6 cm.
< experiments for measurement of items >
The rate of emergence after sowing, the height of seedlings after 1 month of emergence and the root length were measured for each example and blank group, and the results are shown in table 1:
TABLE 1 emergence rate after sowing Miao Temple maple seeds, height and root length after 1 month of emergence
The rate of emergence% Miao height (cm) Root length (cm)
Blank group 35.00 6.13 4.08
Example 1 41.00 6.65 4.13
Example 2 44.00 6.68 4.26
Example 3 52.00 7.83 4.63
As can be seen from table 1, the seedling emergence rate and the seedling height and root length after 1 month of sowing of the temple acer mono-acer seeds cultured by the methods of examples 1 to 3 are superior to those of the blank group, wherein the seedling emergence rate after 1 month of seedling emergence reaches 52%, the seedling height after 1 month of seedling emergence reaches 7.83cm, and the root length after 1 month of seedling emergence reaches 4.63cm, so that a foundation is laid for the subsequent seedling alignment and strengthening of the temple acer mono-acer seedlings.
< comparative example 1>
Firstly, soaking seeds in disinfectant obtained by mixing 12 wt% of Bordeaux mixture and 25 wt% of carbendazim according to the volume ratio of 1:1 for 15min, washing with sterile water, and then airing in a cool and ventilated place;
digging a sand storage pit, wrapping plastic cloth which is sprayed with 0.4 wt% potassium permanganate solution, drying the plastic cloth in the sun for 3d, uniformly mixing wet river sand with the water content of 80% and the dried seeds according to the volume ratio of 1:5 after washing with sterile water, filling the mixture into the sand storage pit, laying a layer of thinner wet river sand on the top, covering a woven bag, keeping moisture and ventilating, turning once every 14d, and spraying a proper amount of sterile water after turning each time until 50% of the seeds have bud spots;
step three, sowing on a seedbed with ridge length of 1.5m, ridge width of 1m, ridge height of 0.25m and step width of 0.6m by adopting a drilling mode of sowing with the width of 10cm, row spacing of 8cm and depth of 6cm, pouring 800 times of liquid medicine of 25 wt% carbendazim wettable powder until water is discharged from the seedbed, and then sowing seeds on the seedbed, wherein the seedbed comprises: a covering layer (formed by pine needle segments, clover powder and hydroxypropyl methylcellulose singly or cooperatively), a matrix soil layer and a perlite layer which are laid in sequence from top to bottom;
the cover layers are respectively provided as:
a: pine needle sections; b: clover powder; c: hydroxypropyl methylcellulose; d: pine needle segments and clover powder; e: pine needle segment, clover powder and hydroxypropyl methylcellulose.
The processing method of the covering layer comprises the following steps:
when the covering layer is A, soaking folium Pini in soap water for 2h, washing, drying, and cutting into 2cm to obtain folium Pini segments;
when the covering layer is B, pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;
when the covering layer is C, 25g of 5 wt% hydroxypropyl methylcellulose is directly added;
when the covering layer is D, stirring and mixing A and B uniformly;
when the covering layer is E, A, B, C is stirred and mixed uniformly.
The matrix soil layer in the third step comprises the following raw materials in parts by weight: 60 parts of brown soil, 40 parts of pine needles, 7 parts of compound microorganisms (wherein 30 parts of propionic acid bacteria and 60 parts of lactobacillus acidophilus are mixed), 15 parts of saw wood residues and 6 parts of EM (effective microorganisms) powder.
The preparation method of the matrix soil layer in the third step comprises the following steps:
adding 600mL of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;
soaking folium Pini in soap water for 2 hr, cleaning, drying, and pulverizing to obtain folium Pini powder;
fermenting the sawn wood residues by using compound microorganisms to obtain a fermentation liquid and fermentation residues;
fully and uniformly stirring the brown soil mud, the pine needle powder, the fermentation liquor, the fermentation residues and the EM bacteria powder, adjusting the pH to 6.6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and then covering a degradable mulching film for 3 days on the surface of the soil to obtain the matrix soil layer.
The emergence rates after sowing of the blank group and comparative example 1 were counted, and the results are shown in table 2 below:
TABLE 2 emergence rate after Miao maple seed sowing
Figure BDA0003736345860000081
Figure BDA0003736345860000091
As can be seen from Table 2, the emergence rate of the Temple maple seeds cultured by the blank group method is 35%, which is relatively low, the emergence rate of the Temple maple seeds cultured by the seedbed formed by A, B, C, D is similar to that of the blank group, and the covering layer A, B, C, D does not play a positive role in promoting the emergence rate. The emergence rate of the temple acer palmatum seeds cultivated by the covering layer E reaches 52%, which shows that the covering layer E has a positive promotion effect on the emergence rate, and the analysis reason is that the pine needle segments and the clover powder greatly increase the water retention effect of the covering layer under the action of hydroxypropyl methylcellulose, have the effects of soil moisture retention and moisture retention on the lower matrix soil layer and further improve the emergence rate of the temple acer palmatum seeds.
< comparative example 2>
Firstly, soaking seeds in disinfectant obtained by mixing 12 wt% of Bordeaux mixture and 25 wt% of carbendazim according to the volume ratio of 1:1 for 15min, washing with sterile water, and then airing in a cool and ventilated place;
digging a sand storage pit, wrapping plastic cloth which is sprayed with 0.4 wt% potassium permanganate solution, drying the plastic cloth in the sun for 3d, uniformly mixing wet river sand with the water content of 80% and the dried seeds according to the volume ratio of 1:5 after washing with sterile water, filling the mixture into the sand storage pit, laying a layer of thinner wet river sand on the top, covering a woven bag, keeping moisture and ventilating, turning once every 14d, and spraying a proper amount of sterile water after turning each time until 50% of the seeds have bud spots;
step three, sowing on a seedbed with ridge length of 1.5m, ridge width of 1m, ridge height of 0.25m and step width of 0.6m by adopting a drilling mode of sowing with the width of 10cm, row spacing of 8cm and depth of 6cm, pouring 800 times of liquid medicine of 25 wt% carbendazim wettable powder until water is discharged from the seedbed, and then sowing seeds on the seedbed, wherein the seedbed comprises: sequentially paving a covering layer (respectively containing original pine needles, 5cm pine needle segments, 2cm pine needle segments and pine needle powder), a matrix soil layer and a perlite layer from top to bottom;
the processing method of the covering layer comprises the following steps:
soaking folium Pini in soap water for 2 hr, washing, drying, and processing to obtain original folium Pini, 5cm folium Pini segment, 2cm folium Pini segment, and folium Pini powder;
pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;
mixing the original pine needle, 5cm pine needle, 2cm pine needle, pine needle powder, clover powder and 25g 5 wt% hydroxypropyl methylcellulose, and forming the covering layer in the seedbed of group 1, group 2, group 3 and group 4.
The matrix soil layer in the third step comprises the following raw materials in parts by weight: 60 parts of brown soil, 40 parts of pine needles, 7 parts of compound microorganisms (wherein 30 parts of propionic acid bacteria and 60 parts of lactobacillus acidophilus are mixed), 15 parts of saw wood residues and 6 parts of EM (effective microorganism) powder.
The preparation method of the matrix soil layer in the third step comprises the following steps:
adding 600mL of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;
soaking folium Pini in soap water for 2 hr, cleaning, drying, and pulverizing to obtain folium Pini powder;
fermenting the sawn wood residues by using compound microorganisms to obtain a fermentation liquid and fermentation residues;
and (3) fully and uniformly stirring the brown soil mud, the pine needle powder, the fermentation liquor, the fermentation residues and the EM powder, adjusting the pH to 6.6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and then covering a degradable mulching film on the surface of the soil for 3d to obtain the matrix soil layer.
The daily temperature difference of the seedbeds of the blank group and the comparative example 2 at different soil layers was measured, and the results are shown in the following table 3:
TABLE 3 daily temperature difference of seedbed at different depth soil layers
Blank group Group 1 Group 2 Group 3 Group 4
Surface of 23.7℃ 22.5℃ 23.2℃ 22.2℃ 23.1℃
10cm 13.0℃ 12℃ 11.9℃ 9.2℃ 12.3℃
20cm 7.2℃ 6.8℃ 6.9℃ 6.7℃ 7.0℃
As can be seen from Table 3, the difference between the daily temperature difference of the four seedbeds in the blank group and the comparative example 2 is not significant at the soil layer of 20cm, and the difference between the daily temperature difference of the surface and the soil layer of 10cm is shown, wherein the daily temperature difference of the seedbed of the group 3 with the covering layer of 2cm pine needle section is the smallest, compared with the seedbed of the blank group, the daily temperature difference is reduced by 4.8 ℃, the reason for analysis is probably that the 2cm pine needle section effectively controls the evaporation speed of the moisture in the seedbed, adjusts the temperature difference in the soil, is beneficial to the respiration of microorganisms, decomposes mineral nutrient elements in the soil, and provides rich nutrient components for the acer seeds.
< comparative example 3>
Firstly, soaking seeds in a disinfectant obtained by mixing 12 wt% of Bordeaux mixture and 25 wt% of carbendazim according to a volume ratio of 1:1 for 15min, washing with sterile water, and then airing in a cool and ventilated place;
digging a sand storage pit, wrapping the aired seeds and plastic cloth which is sprayed by 0.4 wt% of potassium permanganate solution, drying the seeds in the sun for 3 days, uniformly mixing wet river sand with the water content of 80% according to the volume ratio of 1:5 after washing by sterile water, filling the mixture into the sand storage pit, laying a layer of wet river sand on the top, turning the wet river sand once every 14 days, and spraying a proper amount of sterile water after each turning until 50% of the seeds have bud spots;
step three, sowing on a seedbed with ridge length of 1.5m, ridge width of 1m, ridge height of 0.25m and step width of 0.6m by adopting a drilling mode of sowing with the width of 10cm, row spacing of 8cm and depth of 6cm, pouring 800 times of liquid medicine of 25 wt% carbendazim wettable powder until water is discharged from the seedbed, and then sowing seeds on the seedbed, wherein the seedbed comprises: sequentially paving a pine needle-clover mixture layer, a nutrient soil layer and a perlite layer from top to bottom;
the preparation method of the pine needle-clover mixture layer comprises the following steps:
soaking folium Pini in soap water for 2 hr, washing, drying, and cutting into 2cm segments;
pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;
stirring and mixing the pine needle segments, the clover powder and 25g of 5 wt% hydroxypropyl methylcellulose uniformly.
The nutrient soil layer in the third step is formed by respectively adding the following raw materials in parts by weight on the basis of 6 parts of EM (effective microorganism) powder:
group a: 100 parts of brown soil; group b: 78 parts of brown soil, 7 parts of compound microorganism and 15 parts of sawmilling residue; group c: 85 parts of brown soil and 15 parts of pine needles; group d: 60 parts of brown soil, 7 parts of compound microorganisms, 40 parts of pine needles and 15 parts of sawn wood residues;
wherein the compound microorganism is prepared by mixing 30 parts of propionic acid bacteria and 60 parts of lactobacillus acidophilus.
The preparation method of the nutrient soil layer in the third step comprises the following steps:
adding 600mL of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;
soaking folium Pini in soap water for 2 hr, cleaning, drying, and pulverizing to obtain folium Pini powder;
fermenting the sawn wood residues by using compound microorganisms to obtain a fermentation liquid and fermentation residues;
and respectively and fully stirring the group a, the group b, the group c, the group d and the EM bacterial powder uniformly, adjusting the pH to 6.6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and then covering a degradable mulching film 3d on the surface of the mixture to respectively obtain each group of nutrient soil layers.
The quick-acting nutrient contents of the seedbeds of the blank group and the comparative example 4 at a soil layer of 10cm were measured, and the results are shown in table 4:
TABLE 4 quick-acting nutrient content of seedbed in 10cm soil layer
Blank group Group a Group b Group c Group d
Quick-acting nitrogen (mg/kg) 170.82 200.17 237.79 213.56 473.41
Quick-acting phosphorus (mg/kg) 7.85 11.21 15.46 10.97 20.25
Quick-acting calcium (mg/kg) 140.31 315.45 400.52 328.69 450.06
Quick-acting iron (mg/kg) 31.86 33.25 40.58 36.81 62.41
It can be known from table 4 that the content of available nitrogen, available phosphorus, available calcium and available iron nutrients for promoting the growth of seedlings in the nutrient soil layer of group d is higher than that of the blank group, the group a, the group b and the group c, and the analysis reason may be that pine needles are degraded to form humus, and the fermentation liquid generated by the saw wood residue under the action of composite microbial fermentation promotes the calcium, phosphorus and iron in the humus and the fermentation residue to be converted into the seedlings to absorb the available nitrogen, available phosphorus and available calcium, and the available iron and other mineral nutrient elements rich in the brown soil are matched to greatly increase the available nutrients in the nutrient soil layer and provide balanced nutrients for the growth of the seedlings of the acer e pai of the subsequent temple.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.

Claims (8)

1. The seedling growing method of the temple acer mono seeds is characterized by comprising the following steps:
soaking seeds in a disinfectant for 8-15 min, washing with sterile water, and then placing in a cool and ventilated place for airing;
step two, digging a sand storage pit, uniformly mixing the aired seeds with wet river sand with the water content of 60-80% according to the volume ratio of 1: 3-5, filling the mixture into the sand storage pit, laying a layer of thin wet river sand on the top, covering a woven bag, turning the mixture once every 7-14 days, and spraying a proper amount of sterile water after each turning until 30-50% of the seeds have bud spots;
step three, pouring a proper amount of 20-25 wt% carbendazim wettable powder 500-800 times of liquid medicine on a seedbed, and then sowing seeds on the seedbed, wherein the seedbed comprises: sequentially paving a pine needle-clover mixture layer, a matrix soil layer and a perlite layer from top to bottom;
the preparation method of the pine needle-clover mixture layer comprises the following steps:
soaking pine needles in soap water for 1-2 h, then washing, drying and cutting into 1-2 cm to obtain pine needle sections;
pulverizing herba Trifolii Pratentis, soaking in clear water, filtering, and oven drying to obtain herba Trifolii Pratentis powder;
stirring and mixing the pine needle segments, the clover powder and 2-5 wt% of hydroxypropyl methylcellulose uniformly.
2. A method for growing seedlings of temple acer mono seeds as in claim 1, wherein the matrix soil layer in step three comprises the following raw materials in parts by weight: 45-60 parts of brown soil, 30-40 parts of pine needles, 3-7 parts of compound microorganisms, 10-15 parts of sawmilling residues and 2-6 parts of EM (effective microorganism) powder.
3. A method of growing seedlings of temple acer mono seed as claimed in claim 2, wherein the method of preparing the matrix soil layer of step three comprises:
adding a proper amount of water into the brown soil, and stirring until the soil is in a mud state to obtain brown soil mud;
soaking pine needles in soap water for 1-2 hours, cleaning, drying and crushing to obtain pine needle powder;
fermenting the sawn wood residues by using compound microorganisms to obtain a fermentation liquid and fermentation residues;
fully and uniformly stirring the brown soil mud, the pine needle powder, the fermentation liquor, the fermentation residues and the EM bacteria powder, adjusting the pH to 5.5-6.6 by using a saturated potassium carbonate solution and a saturated phosphoric acid solution, airing, and then covering a degradable mulching film on the surface of the soil for 2-3 days to obtain the matrix soil layer.
4. The method for growing seedlings of temple acer mono seeds as in claim 1, wherein the disinfectant in the first step is a solution obtained by mixing 10-12 wt% of bordeaux mixture and 20-25 wt% of carbendazim in a volume ratio of 1: 1.
5. A method for growing seedlings of temple acer mono seeds as in claim 1, further comprising sterilizing the river sand of step two, the specific method is: and (3) spraying 0.2-0.4 wt% of potassium permanganate solution, wrapping the plastic cloth, and airing the plastic cloth in the sun for 2-3 days.
6. The method for growing seedlings of temple acer mono seed as claimed in claim 1, wherein the weight parts of the composite microorganism in the third step are 20-30 parts of propionic acid bacteria and 50-60 parts of lactobacillus acidophilus.
7. The method of claim 1, wherein the third step is a seedbed with a ridge length of 1.2-1.5 m, a ridge width of 0.8-1 m, a ridge height of 0.15-0.25 m, and a step width of 0.4-0.6 m.
8. A method for growing seedlings of temple bench maple seeds according to claim 1, wherein the seeding in step three is drill seeding, the parameters of drill seeding are: the sowing width is 8-10 cm, the row spacing is 5-8 cm, and the depth is 4-6 cm.
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