CN114867850A - 一种纯化ucart细胞的方法与应用 - Google Patents

一种纯化ucart细胞的方法与应用 Download PDF

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CN114867850A
CN114867850A CN202080090615.1A CN202080090615A CN114867850A CN 114867850 A CN114867850 A CN 114867850A CN 202080090615 A CN202080090615 A CN 202080090615A CN 114867850 A CN114867850 A CN 114867850A
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car
cells
cell
tcr
sgrna
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袁鹏飞
王飞
牛立超
付建强
谷丰
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Edigene Biotechnology Inc
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Abstract

一种纯化通用型人类CAR‑T细胞的方法，包括：(i)通过基因编辑技术破坏所述人类CAR‑T细胞中：14号染色体第23016448位至第23016490位的TRAC基因区域；和15号染色体第45003745位至第45003788位的B2M基因区域；(ii)然后将靶向TCRα/β的mRNA导入经基因编辑后的CAR‑T细胞；(iii)再将经过上述处理的CAR‑T细胞群体进行体外培养。

Description

PCT国内申请，说明书已公开。

Claims (30)

  1. PCT国内申请，权利要求书已公开。
CN202080090615.1A 2019-12-30 2020-12-30 一种纯化ucart细胞的方法与应用 Pending CN114867850A (zh)

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