CN114601771A

CN114601771A - Soothing essence and preparation method thereof

Info

Publication number: CN114601771A
Application number: CN202210460848.7A
Authority: CN
Inventors: 陈伟定; 彭鑫东; 陈锋华
Original assignee: Guangzhou Oulan Cosmetics Co ltd
Current assignee: Guangzhou Oulan Cosmetics Co ltd
Priority date: 2022-04-28
Filing date: 2022-04-28
Publication date: 2022-06-10
Anticipated expiration: 2042-04-28
Also published as: CN114601771B

Abstract

The invention relates to the field of cosmetics, and particularly discloses a soothing essence and a preparation method thereof. The soothing essence comprises the following components in percentage by mass: 0.03-0.08% of carboxymethyl beta-dextran sodium; 0.1 to 0.5 percent of carboxymethyl chitosan; 3-8% of gentian root extract; 1-3% of Piper methysticum Forst leaf/root/stem/extract; 0.5-2% of cynanchum atratum extract; 8-10% of a humectant; 0.1 to 0.4 percent of thickening agent; the balance being solvent. The preparation method of the soothing essence comprises the following steps: the components are uniformly mixed according to a certain sequence at a specific temperature to obtain the soothing essence. The soothing essence disclosed by the invention has the advantage of protecting Langerhans cells; in addition, the preparation method of the invention also has the advantages of simple and convenient operation and easy industrial production.

Description

Soothing essence and preparation method thereof

Technical Field

The invention relates to the field of cosmetics, and particularly relates to a soothing essence and a preparation method thereof.

Background

The skin of the human body is the largest organ of the human body, is the first line of defense of the human body, has a protection effect on the damage of microorganisms such as mechanical, physical, chemical and bacteria, and can prevent the loss of nutrient substances and water in the human body. Therefore, protection of the skin is of great importance.

The skin defense against external attacks is mainly achieved by a passive defense system consisting of sebum and lipids of stratum corneum cells, and an active defense system, and the formed barrier can effectively prevent penetration and invasion of foreign objects and microorganisms. The more important active defense system consists of langerhans cells, dendritic cells, which reach the stratum corneum of the epidermal cells, where foreign antigens crossing the stratum corneum are phagocytosed and digested upon encountering the langerhans cells, which expand and recognize and phagocytose the foreign substances by activating the immune system macrophages.

However, the shape and number of langerhans cells and the ability to activate the immune system are easily affected by the irradiation of ultraviolet rays, which tends to cause "holes" in the defense network formed by the langerhans cells, thereby affecting the complete removal of foreign antigens entering the stratum corneum by the langerhans cell tissue and further easily causing the increase of the skin allergy rate. Although there are many soothing and anti-inflammatory products on the market, none of the current products achieve the effect of protecting langerhans cells, and thus there is still room for improvement.

Disclosure of Invention

In order to better protect Langerhans cells, the application provides a soothing essence and a preparation method thereof.

In a first aspect, the application provides a soothing essence, which adopts the following technical scheme:

the soothing essence comprises the following components in percentage by mass:

0.03-0.08% of carboxymethyl beta-glucan sodium;

0.1 to 0.5 percent of carboxymethyl chitosan;

3-8% of gentian root extract;

1-3% of Piper methysticum Forst leaf/root/stem extract;

0.5-2% of cynanchum atratum extract;

8-10% of a humectant;

0.1 to 0.4 percent of thickening agent;

the balance being solvent.

Receptors containing beta-glucan on Langerhans cells are combined with receptors of cell membranes by adding carboxymethyl beta-glucan sodium, and a series of stereochemical changes are easily caused, so that macrophages are activated, various cytokines, epidermal growth factors and angiogenesis factors are produced, an immune system of a human is activated, the Langerhans cells are favorably and better protected, the Langerhans cells are less prone to being damaged when being irradiated by ultraviolet rays, and further, a defense system is favorably and better protects the skin, and the skin is less prone to being allergic or red and swollen.

Meanwhile, the carboxymethyl chitosan, the gentian root extract, the kava leaf/root/stem extract and the carboxymethyl beta-glucan sodium are added for synergistic compounding, so that the compatibility of the carboxymethyl beta-glucan sodium in the soothing essence is better improved, the carboxymethyl beta-glucan sodium is better promoted to play a role, and further Langerhans cells are better protected, and the skin is less prone to the situations of allergy and redness and swelling.

In addition, the carboxymethyl chitosan, the gentian root extract, the kava leaf/root/stem extract and the carboxymethyl beta-glucan sodium are compounded in a synergistic manner, so that the proliferation of macrophages is promoted better, the growth of new cells is promoted better, skin wrinkles are removed better, and the skin wrinkles grow gradually and flatly.

By adding the kava leaf/root/stem extract, the fragrance of the relieving essence can be better adjusted, so that the relieving essence can emit refreshing fragrance and is beneficial to refreshing and soothing the nerves; meanwhile, the use of essence can be reduced, thereby being beneficial to reducing the stimulation or damage of chemical substances to the skin.

Preferably, the soothing essence further comprises the following components in percentage by mass:

0.5-2% of panthenol.

By adding panthenol, the anti-allergy effect of the skin can be better improved to a certain degree, and meanwhile, macrophages can be better activated to generate various cytokines, epidermal growth factors and angiogenesis factors, so that collagen and neovascularization which are necessary for wound healing can be better promoted, and the soothing essence has the effect of repairing skin injury to a certain degree.

arginine 0.1-0.3%.

By adding arginine and panthenol for synergistic compounding, the effect of panthenol is promoted better, so that the effect of the soothing essence for repairing skin injury is better; meanwhile, the growth of cell factors is promoted better, so that the growth of new cells is faster, wrinkles are smoothed better, and the wrinkles of the skin are fewer.

Preferably, the moisturizer comprises one or more of glycerin, sodium hyaluronate, butylene glycol, 1, 2-pentanediol, and 1, 2-hexanediol.

Preferably, the humectant is prepared by mixing glycerol, sodium hyaluronate, butanediol, 1, 2-pentanediol and 1, 2-hexanediol in a mass ratio of 1:0.01-0.05:2-5:2-5: 0.5-1.5.

Preferably, the humectant is prepared by mixing glycerol, sodium hyaluronate, butanediol, 1, 2-pentanediol and 1, 2-hexanediol in a mass ratio of 1:0.03:4:3: 1.

By adopting the components compounded in a specific proportion to be cooperated as the moisturizing agent, the moisturizing effect of the soothing essence can be better improved, and the skin is more moisturized.

Preferably, the thickener comprises one or both of carbomer and xanthan gum.

Preferably, the thickening agent is formed by mixing carbomer and xanthan gum in a mass ratio of 11-15: 5.

Preferably, the thickening agent is formed by mixing carbomer and xanthan gum in a mass ratio of 13: 5.

The components compounded in a specific ratio are used as the thickening agent in a synergistic manner, so that the consistency of the soothing essence can be better adjusted, the components are better compatible, and the components are better uniformly dispersed in the soothing essence and better perform a synergistic effect.

In a second aspect, the application provides a preparation method of a soothing essence, which adopts the following technical scheme:

a preparation method of a soothing essence comprises the following steps:

step 1, adding a solvent, a thickening agent, sodium hyaluronate, glycerol and butanediol into a stirring kettle, uniformly stirring, raising the temperature to 80-85 ℃ to completely dissolve the materials, and keeping the temperature for 25-35min and then beginning to cool;

step 2, when the temperature is reduced to 55-60 ℃, adding arginine, stirring uniformly, continuously reducing the temperature to 45-50 ℃, adding 1, 2-pentanediol, 1, 2-hexanediol, carboxymethyl beta-glucan sodium and carboxymethyl chitosan, stirring uniformly, continuously reducing the temperature to 40-43 ℃, adding panthenol, gentiana scabra bunge extract, kava leaf/root/stem extract and cynanchum atratum extract, stirring uniformly, continuously reducing the temperature to 36-38 ℃, filtering, discharging, and standing to obtain the soothing essence.

By controlling the adding sequence and the adding temperature of the components, the components are favorably compatible and uniformly dispersed in the soothing essence better, so that the components are favorably compounded in a synergistic manner better and play a role better, and the soothing effect and the wrinkle removing effect of the soothing essence are better.

In summary, the present application has the following beneficial effects:

1. by adding the carboxymethyl beta-dextran sodium, better protection of Langerhans cells is facilitated, the Langerhans cells are less prone to damage when being irradiated by ultraviolet rays, and a defense system is further facilitated to better protect the skin.

2. By adding carboxymethyl chitosan, gentian root extract, kava leaf/root/stem extract and carboxymethyl beta-glucan sodium for synergistic compounding, the compatibility of the carboxymethyl beta-glucan sodium in the soothing essence can be better improved, so that Langerhans cells can be better protected.

3. The carboxymethyl chitosan, the gentian root extract, the kava leaf/root/stem extract and the carboxymethyl beta-glucan sodium are compounded in a synergistic manner, so that the proliferation of macrophages is promoted better, the growth of new cells is promoted better, skin wrinkles are removed better, and the skin wrinkles grow gradually and flatly.

4. By adding panthenol and arginine, the method is favorable for better activating macrophages to generate various cytokines, epidermal growth factors and angiogenesis factors, and is favorable for better promoting the repair of skin injury and the growth of new cells, thereby being favorable for better smoothing skin wrinkles.

5. By controlling the adding sequence and the adding temperature of the components, the components are favorably compatible and uniformly dispersed in the soothing essence better, so that the components are favorably compounded in a synergistic manner better and play a role better, and the soothing effect and the wrinkle removing effect of the soothing essence are better.

Detailed Description

The present application will be described in further detail with reference to examples and comparative examples.

The source of the raw materials for the following examples and comparative examples are detailed in table 1.

TABLE 1

Example 1

The embodiment of the application discloses soothing essence, which consists of the following components in parts by mass:

0.03kg of carboxymethyl beta-dextran sodium; 0.1kg of carboxymethyl chitosan; 3kg of gentian root extract; 1kg of kava leaf/root/stem extract; 0.5kg of cynanchum atratum extract; 8kg of humectant; 0.1kg of thickening agent; 87.27kg of solvent.

In this embodiment, the humectant is glycerin; the thickening agent is carbomer; the solvent is water.

The embodiment of the application also discloses a preparation method of the soothing essence, which comprises the following steps:

step 1, accurately weighing the raw material components according to the mass, putting the raw material components into a cleaned and disinfected vessel for later use, cleaning a stirring kettle, sequentially adding water, carbomer and glycerol into the stirring kettle, stirring at a rotating speed of 110r/min, gradually raising the temperature to 80 ℃, completely dissolving the materials in the stirring kettle, and cooling after keeping the temperature for 35 min.

And 2, stirring and cooling under natural conditions, adding carboxymethyl beta-glucan sodium and carboxymethyl chitosan while stirring when the temperature is reduced to 45 ℃, stirring uniformly, then continuing to stir and cool under natural conditions, adding a gentian root extract, a kava leaf/root/stem extract and a cynanchum atratum extract when the temperature is reduced to 40 ℃, stirring and cooling under natural conditions continuously after stirring uniformly, filtering and discharging when the temperature is reduced to 36 ℃, and standing for 3 hours to obtain the soothing essence.

Example 2

0.08kg of carboxymethyl beta-glucan sodium; 0.5kg of carboxymethyl chitosan; 8kg of gentian root extract; 3kg of kava leaf/root/stem extract; 2kg of cynanchum atratum extract; 10kg of humectant; 0.4kg of thickening agent; 76.02kg of solvent.

In the embodiment, the humectant is prepared by uniformly mixing glycerol, sodium hyaluronate, butanediol, 1, 2-pentanediol and 1, 2-hexanediol in a mass ratio of 1:0.01:2:2: 0.5;

the thickening agent is carbomer and xanthan gum, wherein the weight ratio of carbomer to xanthan gum is 11: 5, uniformly mixing the components in a mass ratio;

the solvent is water.

The embodiment of the application further discloses a preparation method of the soothing essence, which comprises the following steps:

step 1, accurately weighing the raw material components according to the mass, putting the raw material components into a cleaned and disinfected vessel for standby, cleaning a stirring kettle, sequentially adding water, carbomer, xanthan gum, sodium hyaluronate, glycerol and butanediol into the stirring kettle, stirring at a rotating speed of 110r/min, gradually raising the temperature to 85 ℃, completely dissolving the materials in the stirring kettle, and cooling after keeping the temperature for 25 min.

And 2, stirring and cooling under natural conditions, adding 1, 2-pentanediol, 1, 2-hexanediol, carboxymethyl beta-glucan sodium and carboxymethyl chitosan while stirring when the temperature is reduced to 50 ℃, uniformly stirring, continuing to stir and cool under natural conditions, adding the gentian root extract, the kava leaf/root/stem extract and the cynanchum atratum extract when the temperature is reduced to 43 ℃, stirring uniformly, continuing to stir and cool under natural conditions, filtering and discharging materials when the temperature is reduced to 38 ℃, and standing for 3 hours to obtain the soothing essence.

Example 3

0.05kg of carboxymethyl beta-dextran sodium; 0.3kg of carboxymethyl chitosan; 5kg of gentian root extract; 2kg of kava leaf/root/stem extract; 1kg of cynanchum atratum extract; 9kg of humectant; 0.2kg of thickening agent; 82.45kg of solvent.

In the embodiment, the humectant is prepared by uniformly mixing glycerol, sodium hyaluronate, butanediol, 1, 2-pentanediol and 1, 2-hexanediol in a mass ratio of 1:0.05:5:5: 1.5;

the thickening agent is carbomer and xanthan gum, wherein the weight ratio of carbomer to xanthan gum is 3:1 by mass ratio;

the solvent is water.

step 1, accurately weighing the raw material components according to the mass, putting the raw material components into a cleaned and disinfected vessel for later use, cleaning a stirring kettle, sequentially adding water, carbomer, xanthan gum, sodium hyaluronate, glycerol and butanediol into the stirring kettle, stirring at a rotating speed of 110r/min, gradually raising the temperature to 83 ℃, completely dissolving the materials in the stirring kettle, and cooling after heat preservation for 30 min.

And 2, stirring and cooling under natural conditions, adding 1, 2-pentanediol, 1, 2-hexanediol, carboxymethyl beta-glucan sodium and carboxymethyl chitosan while stirring when the temperature is reduced to 48 ℃, uniformly stirring, continuing to stir and cool under natural conditions, adding the gentian root extract, the kava leaf/root/stem extract and the cynanchum atratum extract when the temperature is reduced to 42 ℃, stirring uniformly, continuing to stir and cool under natural conditions, filtering and discharging materials when the temperature is reduced to 37 ℃, and standing for 3 hours to obtain the soothing essence.

Example 4

The difference from example 3 is that: the soothing essence also comprises 0.5kg of panthenol. And panthenol is added together with radix Gentianae extract, Piper methysticum leaf/root/stem extract and radix Cynanchi Atrati extract when the temperature is reduced to 42 deg.C in step 2.

Example 5

The difference from example 3 is that: the components of the soothing essence also include 2kg panthenol. And panthenol is added together with radix Gentianae extract, Piper methysticum leaf/root/stem extract and radix Cynanchi Atrati extract when the temperature is reduced to 42 deg.C in step 2.

Example 6

The difference from example 3 is that: the components of the soothing essence also comprise 0.1kg of arginine. And adding arginine when the temperature is reduced to 55 ℃ in the step 2, continuously reducing the temperature to 48 ℃ after uniformly stirring, and adding 1, 2-pentanediol, 1, 2-hexanediol, carboxymethyl beta-dextran sodium and carboxymethyl chitosan.

Example 7

The difference from example 3 is that: the components of the soothing essence also comprise 0.3kg of arginine. And adding arginine when the temperature is reduced to 60 ℃ in the step 2, continuously reducing the temperature to 48 ℃ after uniformly stirring, and adding 1, 2-pentanediol, 1, 2-hexanediol, carboxymethyl beta-dextran sodium and carboxymethyl chitosan.

Example 8

The difference from example 3 is that: the soothing essence also comprises 0.5kg of panthenol and 0.1kg of arginine. And panthenol is added together with radix Gentianae extract, Piper methysticum Forst leaf/root/stem extract and radix Cynanchi Atrati extract when the temperature is reduced to 42 deg.C in step 2; and (3) adding arginine when the temperature is reduced to 55 ℃ in the step (2), stirring uniformly, then continuously reducing the temperature to 48 ℃, and adding 1, 2-pentanediol, 1, 2-hexanediol, carboxymethyl beta-dextran sodium and carboxymethyl chitosan.

Example 9

The difference from example 3 is that: the soothing essence also comprises 2kg of panthenol and 0.3kg of arginine. And panthenol is added together with radix Gentianae extract, Piper methysticum Forst leaf/root/stem extract and radix Cynanchi Atrati extract when the temperature is reduced to 42 deg.C in step 2; and (3) adding arginine when the temperature is reduced to 60 ℃ in the step (2), stirring uniformly, then continuously reducing the temperature to 48 ℃, and adding 1, 2-pentanediol, 1, 2-hexanediol, carboxymethyl beta-dextran sodium and carboxymethyl chitosan.

Example 10

The difference from example 3 is that:

the humectant is prepared by uniformly mixing glycerol, sodium hyaluronate, butanediol, 1, 2-pentanediol and 1, 2-hexanediol in a mass ratio of 1:0.03:4:3: 1;

the thickening agent is carbomer and xanthan gum, wherein the weight ratio of carbomer to xanthan gum is 13:5, and the components are uniformly mixed according to the mass ratio.

The soothing essence also comprises 1kg of panthenol and 0.2kg of arginine. And panthenol is added together with radix Gentianae extract, Piper methysticum Forst leaf/root/stem extract and radix Cynanchi Atrati extract when the temperature is reduced to 42 deg.C in step 2; and (3) adding arginine when the temperature is reduced to 58 ℃ in the step (2), stirring uniformly, then continuously reducing the temperature to 48 ℃, and adding 1, 2-pentanediol, 1, 2-hexanediol, carboxymethyl beta-dextran sodium and carboxymethyl chitosan.

Comparative example 1

The difference from example 3 is that: equal amount of humectant is used to replace carboxymethyl beta-glucan sodium, carboxymethyl chitosan, radix Gentianae extract, Piper methysticum leaf/root/stem/extract and radix Cynanchi Atrati extract.

Comparative example 2

The difference from example 3 is that: equal amount of humectant is used instead of carboxymethyl chitosan, radix Gentianae extract, Piper methysticum leaf/root/stem/extract and radix Cynanchi Atrati extract.

Comparative example 3

The difference from example 3 is that: equivalent humectant is used to replace carboxymethyl beta-dextran sodium.

Comparative example 4

The difference from example 3 is that: equal amount of humectant was substituted for carboxymethyl chitosan.

Comparative example 5

The difference from example 3 is that: equal amount of humectant was substituted for the gentian extract.

Comparative example 6

The difference from example 3 is that: the kava leaves/roots/stems/extracts were replaced with equal amounts of humectant.

Comparative example 7

The difference from example 3 is that: equal amount of humectant is used to replace radix Cynanchi Atrati extract.

Experiment 1

51 female SPF-grade Balb/c mice (purchased from institute of laboratory animals, university of cooperative and medical sciences, China pharmaceutical sciences) with the weight of 20 +/-2 g, which are aged for 8 weeks, are taken and raised for one week, and then the mice are divided into 17 groups of 3 mice each.

Then, local hair on the back of the mouse is shaved by using an electric hair cutter and an electric shaver, two skins with the area of 0.5cm multiplied by 0.5cm are selected as test parts on the back of the mouse, and the two test parts are respectively positioned on two sides of the spine. Then, the soothing essence prepared according to the above embodiment and the comparative preparation is correspondingly smeared on the skin of the test part on one side, the test part on the other side is not treated, and the UV dose is 1.5J/cm²The skin of the test parts on both sides was irradiated with ultraviolet rays for 20 min.

After completion of the irradiation treatment, the mice were anesthetized with ether, and skin tissue specimens were cut on the test sites, after which the wounds of the mice were sutured. Then, the skin tissue surface is scraped with a scalpel to remove sebum and dandruff, and subcutaneous fat is removed. Washing with phosphate buffer solution with pH of 7.4, adding EDTA separating solution, placing in water bath at 37 deg.C for 2 hr, taking out the skin, and separating with sharp forceps under dissecting microscope. The EDTA separation solution consists of the following components in parts by mass: 7.6g of tetrasodium ethylene diamine tetraacetate; 6.83g of sodium chloride; 0.2g of potassium chloride; 1.15g of disodium hydrogen phosphate; potassium dihydrogen phosphate 0.2 g; 1.2mL of 1% phenol red solution; distilled water was added to 1000mL and the pH was adjusted to 7.2.

After separation of true epidermis, Langerhans cells in epidermis are stained, and the specific steps are as follows:

step a, placing the epidermis in acetone for fixing for 10min at room temperature;

step b, taking out the epidermis, and washing the epidermis for 3 times by using phosphate buffer solution with pH of 7.4, wherein each time lasts for 10 min;

step c, putting the cleaned epidermis into an Eppendorf tube containing a 1:50 mouse anti-mouse H-2I-a monoclonal antibody, fully developing the epidermis in the antibody by using forceps, and standing for one night at the temperature of 4 ℃;

d, taking out the epidermis the next day, washing the epidermis for 3 times with phosphate buffer solution with the pH value of 7.4, 10min each time, putting 1:100Vectastain ABC reagent after washing, and incubating for 1.5h at 37 ℃;

step e, taking out the epidermis and washing the epidermis for 3 times by using phosphate buffer solution with the pH value of 7.4, wherein each time lasts for 10 min;

and f, adding the washed epidermis into the DAB kit, observing under a microscope, controlling the color development time to be 10min, washing with distilled water, and stopping the color development reaction.

And step g, paving the slices under a dissecting microscope, sealing the slices with glycerol gelatin, and observing the slices under the microscope.

The reagents used in the above experiments were as follows: biotin labeling of mouse anti-murine H-2I-Ad antibody (accession number 550554, BD PharMigen USA); 1:100 Vector ABC reagent (Vector Laboratories, Inc., U.S. A); DAB kit (purchased from gold bridge of China fir, Beijing, No. ZLI-9032).

Observing the stained slide under a microscope, wherein dendritic cells with brownish brown cell membranes and/or cytoplasms are Langerhans cells, randomly selecting 5 discontinuous fields for each slide under a 400-fold optical microscope, counting the number of positive cells in squares, and calculating the cell density (cells/mm) in unit area²) Firstly, the average value of the cell density obtained by calculation in 5 discontinuous visual fields of the same mouse is taken, and then the average value of the cell density of the skin tissue at the same side of a plurality of mice in the same group is calculated and recorded.

Experiment 2

The soothing essence prepared in the above examples and comparative examples was applied to the wound suture sites of mice in experiment 1,2 times a day, 1 time in the morning and evening, and 1mL each time, and the wound healing conditions of the mice in each group were observed and recorded.

Experiment 3

170 female volunteers with similar skin conditions are randomly selected, the age of the volunteers is 25-45 years, the female volunteers are randomly divided into 17 groups, the soothing essence prepared by the embodiment and the proportion is respectively and correspondingly used in each group, the soothing essence is uniformly applied to the cheek, and the female volunteers are used for 2 times every day, once in the morning and evening, 2mL each time and 30 days after insisting on. The following tests were also performed on the volunteers, and the average of the test results was recorded for each group of volunteers:

skin wrinkles of the volunteers before and after the anti-aging emulsion is used for 30 days are detected by adopting a skin rapid three-dimensional imaging system PRIMOS, and the change rate (%) of the skin wrinkles before and after the soothing essence is used is calculated and recorded. Wherein, the first and the second end of the pipe are connected with each other,

wherein, the smaller the value obtained by detecting skin wrinkles, the fewer wrinkles.

The data from the above experiments are detailed in table 2.

TABLE 2

According to the comparison of the data of the example 3 and the comparative examples 1-2 in the table 2, the comparative example 2 is obtained by adding the carboxymethyl beta-dextran sodium on the basis of the comparative example 1, so that the effect of protecting the Langerhans cells of the soothing essence is improved, and the Langerhans cells are not easily damaged by ultraviolet rays; meanwhile, the preparation method is beneficial to activating macrophages to generate cytokines, epidermal growth factors and angiogenesis factors to a certain extent, so that the wound healing is promoted better, and the generation of wrinkles is delayed better. However, the effects of protecting langerhans cells, promoting wound healing and delaying wrinkle generation of comparative example 2 are all inferior to those of example 3 to a certain extent, which indicates that the problem of compatibility exists when carboxymethyl beta-dextran sodium is added alone, so that the effect of carboxymethyl beta-dextran sodium is easily influenced to a certain extent.

As can be seen from comparison of the data of example 3 with comparative example 1 and comparative examples 3 to 7 in table 2, the effect of protecting langerhans cells, the effect of promoting wound healing and the effect of delaying the generation of wrinkles of the soothing serum were affected regardless of the absence of any of the substances.

Comparison of the data from examples 3-5 in Table 2 shows that the addition of panthenol facilitates better activation of macrophages to produce epidermal growth factor and angiogenic factors, and thus better wound healing.

According to the comparison of the data of example 3 and examples 6-9 in table 2, the synergistic combination of panthenol and arginine is beneficial to activating macrophages to produce cytokines, epidermal growth factors and angiogenesis factors, so that the wound healing and wrinkle removal are promoted.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. The soothing essence is characterized in that: the paint comprises the following components in percentage by mass:

0.03-0.08% of carboxymethyl beta-glucan sodium;

0.1 to 0.5 percent of carboxymethyl chitosan;

3-8% of gentian root extract;

1-3% of Piper methysticum Forst leaf/root/stem/extract;

0.5-2% of cynanchum atratum extract;

8-10% of a humectant;

0.1 to 0.4 percent of thickening agent;

the balance being solvent.

2. The soothing essence of claim 1, wherein: the relieving essence also comprises the following components in percentage by mass:

0.5-2% of panthenol.

3. A soothing serum as claimed in claim 2, wherein: the soothing essence also comprises the following components in percentage by mass:

arginine 0.1-0.3%.

4. A soothing serum according to any one of claims 1 to 3, wherein: the humectant comprises one or more of glycerin, sodium hyaluronate, butanediol, 1, 2-pentanediol and 1, 2-hexanediol.

5. The soothing essence of claim 4, wherein: the humectant is prepared by mixing glycerol, sodium hyaluronate, butanediol, 1, 2-pentanediol and 1, 2-hexanediol in a mass ratio of 1:0.01-0.05:2-5:2-5: 0.5-1.5.

6. The soothing essence of claim 5, wherein: the humectant is prepared by mixing glycerol, sodium hyaluronate, butanediol, 1, 2-pentanediol and 1, 2-hexanediol in a mass ratio of 1:0.03:4:3: 1.

7. A soothing serum according to any one of claims 1 to 3, wherein: the thickening agent comprises one or two of carbomer and xanthan gum.

8. The soothing essence of claim 7, wherein: the thickening agent is formed by mixing carbomer and xanthan gum in a mass ratio of 11-15: 5.

9. The soothing essence of claim 8, wherein: the thickening agent is formed by mixing carbomer and xanthan gum in a mass ratio of 13: 5.

10. A preparation method of a soothing essence is characterized by comprising the following steps: the method comprises the following steps:

step 1, adding a solvent, a thickening agent, sodium hyaluronate, glycerol and butanediol into a stirring kettle, uniformly stirring, raising the temperature to 80-85 ℃ to completely dissolve the sodium hyaluronate, and preserving the temperature for 25-35min and then beginning to cool;