CN113876609A - Anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein and application thereof - Google Patents
Anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein and application thereof Download PDFInfo
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- CN113876609A CN113876609A CN202111271967.XA CN202111271967A CN113876609A CN 113876609 A CN113876609 A CN 113876609A CN 202111271967 A CN202111271967 A CN 202111271967A CN 113876609 A CN113876609 A CN 113876609A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
- A61Q1/12—Face or body powders for grooming, adorning or absorbing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The invention discloses an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein and application thereof. The anti-aging composition comprises, by weight, 0.1-20 parts of hydroxypropyl tetrahydropyrane triol, 0.1-20 parts of snake venom peptide, 0.1-20 parts of recombinant human III type collagen, 5-30 parts of butanediol and 20-70 parts of deionized water. The anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein can be applied to various skin care formulations such as essence water, essence liquid, emulsion, cream and the like. The anti-aging composition has complementary action mechanisms, and has the effects of increasing skin elasticity, promoting collagen synthesis, promoting regeneration of damaged tissues and tightening skin by combined application.
Description
Technical Field
The invention relates to a skin care product and application thereof, belongs to the technical field of daily chemicals, and particularly relates to an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein and application thereof in a skin care product.
Background
Skin aging causes changes in the expression level of skin genes, which affects the integrity of the skin's ultra-fine structure, resulting in dry, loose skin and the appearance of wrinkles. The formation of skin wrinkles, which are associated with skin laxity, hypodermis loss, skeletal atrophy, repeated contraction of expression muscles, etc., may appear and worsen with age. With the gradual aging of China's society, anti-aging is more and more valued by people. Botulinum toxin was used in cosmetic plastic surgery in the last 90's and its effectiveness in wrinkle reduction and cosmetics was also demonstrated.
Under the action of botulinum toxin, the facial and cervical expression muscles are relaxed, dynamic wrinkles caused by contraction of the expression muscles are reduced or eliminated, and the development of the wrinkles can be delayed to a certain extent to achieve the effect of youthfulness. But it also has very obvious side effects, and the common adverse reactions of botulinum toxin injection include local pain, blood stasis, eye redness, gastrointestinal symptoms, eyelid ptosis, blepharoptosis, strabismus, lipvalgus and the like. The injection of botulinum toxin belongs to micro plastic medical cosmetology, and the effect and the safety of the injection are limited by medical conditions, so that the risk is high.
Therefore, people who love beauty are eagerly in need of a safer, effective and convenient anti-aging method. The common anti-aging cosmetics are mild and safe compared with botulinum toxin, but the anti-aging active ingredients are single, are added in some cosmetics only by concepts, and even if the anti-aging cosmetics are sufficient in amount, the transdermal absorbability and the physiological activity of the cosmetics cannot be considered, so that the effect of the cosmetics cannot meet the expectation of consumers, and the anti-aging and wrinkle-removing effects are far less obvious than that of the botulinum toxin.
Whether male or female, there are some obvious changes in the epidermis and dermis with age, which are mainly reflected in the following aspects: the rate of keratinocyte metabolism is slowed, intercellular lipids are reduced, the epidermis is thinned, defense is weakened, TEWL is increased, pigmentation, uneven skin color, the epidermal-dermal junction is weakened, proteins in the dermis are crosslinked and reduced, and the number and activity of fibroblasts with loss of elasticity in the skin are reduced.
It is generally believed that the effects of anti-skin aging actives include: promoting synthesis of mucopolysaccharide and collagen, promoting regeneration of damaged tissue, increasing skin elasticity, and tightening skin.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein, the anti-aging composition disclosed by the invention is complementary in action mechanism, and has the effects of increasing skin elasticity, promoting collagen synthesis, promoting regeneration of damaged tissues and tightening skin through combined application.
The invention also aims to provide a preparation method and application of the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein, the process is simple, the production time is saved, the stability of active ingredients can be kept in the preparation process, and the composition has obvious anti-aging and wrinkle-removing effects.
The technical scheme adopted by the invention is as follows:
an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein comprises the following raw material components in parts by weight:
0.1-20 parts by weight of hydroxypropyl tetrahydropyrane triol;
0.1-20 parts of snake venom peptide;
0.1 to 20 weight portions of recombinant human III type collagen;
5-30 parts of butanediol;
20-70 parts of deionized water.
Further, the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein is characterized by comprising the following raw material components in parts by weight:
1-20 parts of hydroxypropyl tetrahydropyrane triol;
1-10 parts of snake venom peptide;
2-10 parts of recombinant human type III collagen;
5-15 parts of butanediol;
40-65 parts of deionized water.
In addition, the invention also discloses a preparation method of the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein, which comprises the following steps:
1) dissolving hydroxypropyl tetrahydropyrane triol and butanediol in deionized water, and uniformly stirring to prepare a hydroxypropyl tetrahydropyrane triol solution, namely a component A;
2) adding snake venom peptide, recombinant human III type collagen and the component A into deionized water in sequence, and stirring uniformly to obtain the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein.
Further, in the step 1), the stirring speed is 20-80r/min, and in the step 2), the stirring speed is 30-100 r/min.
Further, in the step 2), the mass ratio of the total mass of the 3 raw materials of the hydroxypropyl tetrahydropyrane triol, the snake venom peptides and the recombinant human type III collagen to the mass of the water is 1: 0.5-2.5.
Furthermore, the mass ratio of the total mass of the 3 raw materials of the hydroxypropyl tetrahydropyrane triol, the snake venom peptide and the recombinant human type III collagen to the water is 1: 1.8.
An anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein is applied to a skin care product, wherein the skin care product contains 10-40% of the anti-aging composition according to the total weight of the skin care product; and 60-90% of conventional auxiliary materials in the field of skin care products.
Further, the conventional auxiliary materials comprise a surfactant, a thickening agent, a conditioning agent, a humectant, a preservative, a chelating agent, essence and deionized water.
Further, the skin care product is prepared into various dosage forms of toner, smoothing toner, firming toner, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, facial mask, pressed powder, body lotion, body cream, body oil, body lotion and body essence.
Further, the skin care product is prepared into anti-aging wrinkle-lightening essence which comprises the following raw material components:
the anti-aging wrinkle-lightening essence is prepared by the following steps:
1) mixing deionized water, sodium hyaluronate, xanthan gum and allantoin raw materials, heating at 85 deg.C for 30min, and stirring at 50r/min for 10min to obtain phase A raw material;
2) cooling the phase A raw material to below 40 ℃, adding an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein, an oat extract and a preservative into the phase A raw material, stirring the mixture for 5min at a speed of 70r/min, cooling the mixture to below 35 ℃, and subpackaging the mixture to obtain the anti-aging wrinkle-lightening essence.
The antiseptic is selected from polyols such as hexanediol and phenoxyethanol.
Compared with the prior art, the invention has the beneficial effects that:
1) the anti-aging composition provided by the invention simultaneously contains polypeptide proteins such as hydroxypropyl tetrahydropyrane triol, snake venom peptide, recombinant human III type collagen and the like, the action mechanisms of the polypeptide proteins are complementary, and the anti-aging and wrinkle-removing effects are better when the polypeptide proteins are combined.
2) The anti-aging composition containing the hydroxypropyl tetrahydropyrane triol and the polypeptide protein, provided by the invention, has the advantages of simple preparation method, production time saving, capability of keeping the stability of active ingredients in the preparation process and obvious anti-aging and wrinkle-removing effects.
3) The anti-aging composition containing the hydroxypropyl tetrahydropyrane triol and the polypeptide protein is safe and non-irritant, and is easy to add into formula systems of various formulations of cosmetics, so that the anti-aging composition has important practical significance and wide market prospect.
Drawings
FIG. 1 is a diagram of the action mechanism of snake venom peptide in the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein provided by the invention.
FIG. 2 is a structural diagram of recombinant human type III collagen in the antiaging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein provided by the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
The invention discloses an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein, which is prepared by taking hydroxypropyl tetrahydropyrane triol, a polypeptide protein composition (snake venom peptide, recombinant human III type collagen) and butanediol as raw materials and combining the raw materials according to a proper weight ratio.
The technical effects of the raw material components are described below.
(iii) hydroxypropyl Tetrahydropyranetriol
Hydroxypropyl tetrahydropyrane triol, a xylose derivative derived from western european beech wood, was an anti-aging active ingredient first developed by the lancome laboratory, has the efficacy of promoting the synthesis of collagen and glycosaminoglycan, and helps the dermis maintain elasticity by targeting at the junction DEJ of the epidermis and the dermis.
② snake venom peptide (dipeptide diamino butyryl benzyl amide diacetate)
The standard Chinese names for snake venom peptides are: dipeptide diaminobutyrylbenzylamide diacetate is a synthetic peptide simulating snake venom serum, and can prevent acetylcholine nerve conduction, achieve the effect of relaxing muscles, and further improve dynamic wrinkles. The mechanism of action of snake venom peptides is shown in FIG. 1.
③ recombined human III type collagen
The skin age is determined by collagen, recombinant human III-type collagen is derived from innovative collagen of a biological fermentation technology, human collagen genes are used as templates, optimization and reconstruction are carried out, pichia pastoris is introduced, and fermentation induction, separation and purification are carried out to obtain the collagen. The obtained collagen is highly consistent with human collagen, and has good skin affinity and histocompatibility. Safety, efficacy and human body tests prove that the recombinant human III-type collagen is safe and efficient collagen which can be directly utilized by skin, the skin penetration rate reaches 80-90%, the transdermal process is safe, non-irritant, rapid and lasting, and the recombinant human III-type collagen has a strengthening effect on the stratum corneum.
The structure of the recombinant human type III collagen is shown in figure 2, and the functions are as follows:
1. reconstructing a cell water channel and an ultrastructure, maintaining cell and blood vessel reconstruction, and inhibiting apoptosis of an aging cell; 2. inhibiting inflammatory injury; 3. reverting extracellular matrix reconstruction; 4. perfecting the skin mucosal barrier.
The anti-aging composition containing the hydroxypropyl tetrahydropyrane triol and the polypeptide protein has the effects of increasing skin elasticity, promoting collagen synthesis, promoting damaged tissue regeneration and tightening skin through the difference of action mechanisms among the components, the enhancement of the permeability of the anti-aging composition containing the hydroxypropyl tetrahydropyrane triol and the polypeptide protein by using the humectants such as butanediol and the like and the synergistic effect of the components.
In the following examples, the unit of parts by weight may be g or kg.
All the raw material components of the invention are commercial products. Hydroxypropyl tetrahydropyran triol is available from Shanghai Keqin science and technology, Inc., and snake venom peptide is available from Dismann. The recombinant human III-type collagen with the molecular weight of 5000-. Oat extract was purchased from demi.
Example 1
The embodiment provides an anti-aging wrinkle-lightening essence which comprises the following raw material components in parts by weight:
57.7 parts by weight of deionized water;
40 parts by weight of an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein;
0.05 part by weight of sodium hyaluronate;
0.05 part by weight of xanthan gum;
0.2 part by weight of allantoin;
1 part by weight of oat extract;
1 part by weight of phenoxyethanol;
the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein comprises the following raw material components:
16 parts of hydroxypropyl tetrahydropyrane triol;
10 parts of snake venom peptide;
8 parts of recombinant human III type collagen;
15 parts of butanediol;
51 parts of deionized water;
the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein is prepared by the following method:
1) dissolving hydroxypropyl tetrahydropyrane triol and butanediol in deionized water, and uniformly stirring at the stirring speed of 50r/min to prepare a hydroxypropyl tetrahydropyrane triol solution, namely a component A;
2) adding snake venom peptide, recombinant human III type collagen and the component A into deionized water in sequence, and stirring uniformly at the stirring speed of 40r/min to obtain the anti-aging composition of the anti-aging hydroxypropyl tetrahydropyrane triol and the polypeptide protein.
The preparation method of the anti-aging wrinkle-lightening essence comprises the following steps:
1) mixing deionized water, sodium hyaluronate, xanthan gum and allantoin raw materials, heating at 85 deg.C for 30min, and stirring at 50r/min for 10min to obtain phase A raw material;
2) cooling the phase A raw material to below 40 ℃, adding an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein, an oat extract and a preservative into the phase A raw material, stirring the mixture for 5min at a speed of 70r/min, cooling the mixture to below 35 ℃, and subpackaging the mixture to obtain the anti-aging wrinkle-lightening essence.
Example 2
The embodiment provides an anti-aging wrinkle-lightening essence which comprises the following raw material components in parts by weight:
67.7 parts by weight of deionized water;
30 parts by weight of an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein;
0.05 part by weight of sodium hyaluronate;
0.05 part by weight of xanthan gum;
0.2 part by weight of allantoin;
1 part by weight of oat extract;
1 part by weight of hexanediol;
the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein comprises the following raw material components:
16 parts of hydroxypropyl tetrahydropyrane triol;
10 parts of snake venom peptide;
8 parts of recombinant human III type collagen;
15 parts of butanediol;
51 parts by weight of deionized water;
the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein is prepared by the following method:
1) dissolving hydroxypropyl tetrahydropyrane triol and butanediol in deionized water, and uniformly stirring at the stirring speed of 50r/min to prepare a hydroxypropyl tetrahydropyrane triol solution, namely a component A;
2) adding snake venom peptide, recombinant human III type collagen and the component A into deionized water in sequence, and stirring uniformly at the stirring speed of 40r/min to obtain the anti-aging hydroxypropyl tetrahydropyrane triol and polypeptide protein composition.
The preparation method of the anti-aging wrinkle-lightening essence comprises the following steps:
1) mixing deionized water, sodium hyaluronate, xanthan gum and allantoin raw materials, heating at 85 deg.C for 30min, and stirring at 50r/min for 10min to obtain phase A raw material;
2) cooling the phase A raw material to below 40 ℃, adding an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein, an oat extract and a preservative into the phase A raw material, stirring the mixture for 5min at a speed of 70r/min, cooling the mixture to below 35 ℃, and subpackaging the mixture to obtain the anti-aging wrinkle-lightening essence.
Example 3
The embodiment provides an anti-aging wrinkle-lightening essence which comprises the following raw material components in parts by weight:
87.7 parts by weight of deionized water;
10 parts by weight of an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein;
0.05 part by weight of sodium hyaluronate;
0.05 part by weight of xanthan gum;
0.2 part by weight of allantoin;
1 part by weight of oat extract;
1 part by weight of phenoxyethanol;
the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein comprises the following raw material components:
16 parts of hydroxypropyl tetrahydropyrane triol;
10 parts of snake venom peptide;
8 parts of recombinant human III type collagen;
15 parts of butanediol;
51 parts of deionized water;
the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein is prepared by the following method:
1) dissolving hydroxypropyl tetrahydropyrane triol and butanediol in deionized water, and uniformly stirring at the stirring speed of 50r/min to prepare a hydroxypropyl tetrahydropyrane triol solution, namely a component A;
2) adding snake venom peptide, recombinant human III type collagen and the component A into deionized water in sequence, and stirring uniformly at the stirring speed of 40r/min to obtain the anti-aging hydroxypropyl tetrahydropyrane triol and polypeptide protein composition.
The preparation method of the anti-aging wrinkle-lightening essence comprises the following steps:
1) mixing deionized water, sodium hyaluronate, xanthan gum and allantoin raw materials, heating at 85 deg.C for 30min, and stirring at 50r/min for 10min to obtain phase A raw material;
2) cooling the phase A raw material to below 40 ℃, adding an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein, an oat extract and a preservative into the phase A raw material, stirring the mixture for 5min at a speed of 70r/min, cooling the mixture to below 35 ℃, and subpackaging the mixture to obtain the anti-aging wrinkle-lightening essence.
Blank example 1
The blank example 1 is different from the example 1 only in the raw material components of the anti-aging wrinkle-lightening essence, the anti-aging wrinkle-lightening essence in the comparative example does not contain the anti-aging composition of hydroxypropyl tetrahydropyrane triol and polypeptide protein, and the other operations are the same as the example 1.
Test example for Effect verification
First, safety test-Patch test
Test subjects: the anti-aging wrinkle-lightening essence obtained in examples 1-3 and the anti-aging wrinkle-lightening essence obtained in blank example 1.
The number of the tested persons is as follows: 150, male and female halves, and the age between 20 and 55 years, all meet the subject criteria.
The test process comprises the following steps: the number of test subjects who met the standard of the test subjects was 150, and the test subjects were randomly divided into 5 groups, each of which corresponds to 30 subjects of the anti-wrinkle essence obtained in examples 1 to 3, the anti-wrinkle essence obtained in blank example 1, and the control group.
The anti-aging and wrinkle-lightening essence in the test object is respectively put into the small chambers of the spot tester, the dosage is about 0.020mL-0.025mL, and no substance is put into the control holes.
The spot tester with the test substance is applied to the back or the forearm curvature of the subject with hypoallergenic tape (manufacturer: Shanghai medical apparatus and instruments (group) Co., Ltd., hygienic materials factory, product number of spot test tape: 09000251116), and is applied to the skin with palm gently for 24 h; skin reactions were observed at 30min, 24h, and 48h after removal of the test article blotter according to the criteria shown in table 1 and the observations were recorded, with the ranking criteria shown in table 1 and the test results shown in table 2.
TABLE 1 skin occlusion Patch test skin response grading Standard
TABLE 2 test results of the skin patches on human body of examples 1-3 and blank example 1 of the present invention
As can be seen from Table 2, the anti-aging wrinkle-lightening essence obtained in examples 1-3 and blank example 1 of the present invention is non-irritating to the skin.
Second, efficacy test
Test subjects: the anti-aging composition of example 1 contains hydroxypropyl tetrahydropyrane triol and polypeptide protein.
The anti-aging wrinkle-lightening essence obtained in examples 1-3 and the anti-aging wrinkle-lightening essence obtained in blank example 1.
1) Percent oxygen radical inhibition test
The photon energy of ultraviolet rays is absorbed by atoms or molecules of cells, causing the state of the electronic energy level of the atoms to change, generating a large amount of free radicals, which can hinder the normal development of the cells, interfere with the metabolism of the cells, destroy mitochondria in the cells, and the like.
The test method comprises the following steps: using Organotypical skin model
The skin model is pretreated by the anti-aging wrinkle-lightening essence of the invention, Ve and Vc are control groups, and ultraviolet irradiation with different intensities (0-30 mJ/cm)2UVB intensity), the percent oxygen radical inhibition was measured and the results are shown in table 3.
TABLE 3 test results
As can be seen from table 3, the anti-aging wrinkle-lightening essence obtained in examples 1 to 3 of the present invention can effectively reduce oxygen radicals caused by ultraviolet rays; the anti-aging and wrinkle-reducing essence obtained in blank example 1 has a weak ability of reducing oxygen radicals caused by ultraviolet rays. The anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein can effectively reduce oxygen free radicals caused by ultraviolet rays.
2) Experiment for promoting proliferation of human keratinocyte (hacat cell)
When people study body aging and skin aging, people mostly stress the aging of skin fibroblasts. In recent years, it has been gradually recognized that keratinocytes also play a very important role in skin aging. With age, skin aging is manifested by a slowing of epidermal turnover, a diminished barrier function, and a diminished keratinocyte viability.
The test method comprises the following steps: establishing a keratinocyte in-vitro cytology model, culturing Hacat cells in a 24-well plate, adding 0.1 percent, 0.3 percent and 0.5 percent of the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein in example 1, and adding hoechst3342 nucleic acid dye after 24 hours. Incubate for 15 minutes and then take pictures. The results are shown in Table 4.
TABLE 4 test results
As can be seen from Table 4, the anti-aging composition containing 0.1%, 0.3%, 0.5% of hydroxypropyl tetrahydropyrane triol and polypeptide protein obtained in example 1 of the present invention can effectively promote the proliferation of hacat cells.
3) Synergistic Experimental example
Comparative examples 1 to 6
The anti-aging wrinkle-lightening essence comprises the following raw materials in different proportions, as shown in table 5.
TABLE 5 COMPARATIVE EXAMPLES 1 to 6 raw material composition ratios (unit: parts by weight)
The preparation process was as described in example 1 above.
The anti-wrinkle effect test was performed on example 1 and comparative examples 1 to 6, and the test results after one month are shown in table 6.
TABLE 6 test results
As can be seen from the experimental data in table 6, the anti-wrinkle effects are ranked as example 1> comparative example 4> comparative example 5> comparative example 6> comparative example 1> comparative example 2> comparative example 3, demonstrating that the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein has better anti-aging effect than the single one or two component combination through the synergistic effect between the components.
Third, human body trial evaluation experiment
Test subjects: the anti-aging wrinkle-lightening essence obtained in examples 1-3, the anti-aging wrinkle-lightening essence obtained in blank example 1, and a commercially available anti-aging wrinkle-lightening essence. The essence is adopted for testing: 150 tested persons, 30 tested persons in each group, are respectively numbered 1-30, and the tested persons are used for 2 times in 1 day for 1 month continuously.
Skin elasticity was measured using a skin elasticity tester MPA580 from CK, germany, using a Reviscometer RV600 probe. The wrinkle, the compactness, the fineness and the glossiness are all measured by a skin detector, and particularly, a Visia skin detector is adopted.
TABLE 7 test results
As can be seen from the test data in table 7, the anti-wrinkle essence obtained in examples 1 to 3 of the present invention can effectively improve the wrinkle area of the skin, reduce wrinkles, and simultaneously, the elasticity, firmness and fineness of the skin are significantly improved, and the effect is superior to that of the blank example 1 and the commercially available anti-wrinkle essence.
In conclusion, the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein can promote collagen synthesis, promote regeneration of damaged tissues, increase skin elasticity, improve skin firmness and smoothness and fade skin wrinkles.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. An anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein is characterized in that: the anti-aging composition comprises the following raw material components in parts by weight:
0.1-20 parts by weight of hydroxypropyl tetrahydropyrane triol;
0.1-20 parts of snake venom peptide;
0.1 to 20 weight portions of recombinant human III type collagen;
5-30 parts of butanediol;
20-70 parts of deionized water.
2. The anti-aging composition comprising hydroxypropyl tetrahydropyrane triol, polypeptide protein according to claim 1, wherein: the anti-aging composition comprises the following raw material components in parts by weight:
1-20 parts of hydroxypropyl tetrahydropyrane triol;
1-10 parts of snake venom peptide;
2-10 parts of recombinant human type III collagen;
5-15 parts of butanediol;
40-65 parts of deionized water.
3. The method for preparing an antiaging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein according to claim 1 or 2, characterized by comprising the steps of:
1) dissolving hydroxypropyl tetrahydropyrane triol and butanediol in deionized water, and uniformly stirring to prepare a hydroxypropyl tetrahydropyrane triol solution, namely a component A;
2) adding snake venom peptide, recombinant human III type collagen and the component A into deionized water in sequence, and stirring uniformly to obtain the anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein.
4. The method of claim 3, wherein: in the step 1), the stirring speed is 20-80r/min, and in the step 2), the stirring speed is 30-100 r/min.
5. The method of claim 3, wherein: in the step 2), the mass ratio of the total mass of the 3 raw materials of the hydroxypropyl tetrahydropyrane triol, the snake venom peptide and the recombinant human type III collagen to the mass of the water is 1: 0.5-2.5.
6. The method of claim 5, wherein: the mass ratio of the total mass of 3 raw materials of the hydroxypropyl tetrahydropyrane triol, the snake venom peptide and the recombinant human type III collagen to the mass of water is 1: 1.8.
7. Use of an anti-aging composition comprising hydroxypropyl tetrahydropyrane triol and polypeptide protein according to claim 1 or 2 in skin care products, wherein: the skin care product comprises 10-40% of the anti-aging composition by total weight of the skin care product; and 60-90% of conventional auxiliary materials in the field of skin care products.
8. The use of an anti-aging composition comprising hydroxypropyl tetrahydropyrane triol and a polypeptide protein according to claim 7 in a skin care product, wherein:
the conventional auxiliary materials comprise a surfactant, a thickening agent, a conditioning agent, a humectant, a preservative, a chelating agent, essence and deionized water.
9. The use of an anti-aging composition comprising hydroxypropyl tetrahydropyrane triol and a polypeptide protein according to claim 7 in a skin care product, wherein:
the skin care product is prepared into various dosage forms of toner, smoothing toner, firming toner, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, facial mask, pressed powder, body lotion, body cream, body oil, body lotion and body essence.
10. The use of an anti-aging composition comprising hydroxypropyl tetrahydropyrane triol and a polypeptide protein according to claim 7 in a skin care product, wherein:
the skin care product is prepared into anti-aging wrinkle-lightening essence which comprises the following raw material components:
10-40 parts by weight of anti-aging hydroxypropyl tetrahydropyrane triol and polypeptide protein composition;
the anti-aging wrinkle-lightening essence is prepared by the following steps:
1) mixing deionized water, sodium hyaluronate, xanthan gum and allantoin raw materials, heating at 85 deg.C for 30min, and stirring at 50r/min for 10min to obtain phase A raw material;
2) cooling the phase A raw material to below 40 ℃, adding an anti-aging composition containing hydroxypropyl tetrahydropyrane triol and polypeptide protein, an oat extract and a preservative into the phase A raw material, stirring the mixture for 5min at a speed of 70r/min, cooling the mixture to below 35 ℃, and subpackaging the mixture to obtain the anti-aging wrinkle-lightening essence.
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