CN108653084B

CN108653084B - Growth factor-containing skin care composition for men and application thereof

Info

Publication number: CN108653084B
Application number: CN201810658839.2A
Authority: CN
Inventors: 陈松彬; 倪彦艳; 陈健欢; 郑涌镟
Original assignee: Guangdong Cooway Biological Technology Co ltd
Current assignee: Guangdong Cooway Biological Technology Co ltd
Priority date: 2018-06-25
Filing date: 2018-06-25
Publication date: 2020-11-24
Anticipated expiration: 2038-06-25
Also published as: CN108653084A

Abstract

The invention discloses a male skin care composition containing growth factors and application thereof, wherein the male skin care composition comprises the following components: basic fibroblast growth factor, water-soluble fullerene, hydrolyzed rice protein, alpha-mannan, a Leucojum aestivum bulb extract and a bergamot extract. The water-soluble fullerene is keratan sulfate oligosaccharide-fullerene C₆₀A complex of fullerene C in said water-soluble fullerene₆₀The mass percentage of the catalyst is 4-8%. The skin care composition is designed aiming at the skin of men, has remarkable effects of moisturizing, controlling oil, resisting aging, removing wrinkles and the like, can be applied to the preparation of men's skin care products such as toner, emulsion, cream, mask, essence and the like, can expand the market of the men's skin care products, and meets the increasing demands on the marketIncreasing men's skin care needs.

Description

Growth factor-containing skin care composition for men and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a male skin care composition containing growth factors and application thereof.

Background

With the development and the updating of the concept of society, people pay more attention to the external appearance and physical maintenance. Not only women are keen about the face problem, but more and more men begin to expand rapidly by the face-protecting concept. The demand of modern men on skin care products is increased year by year, the skin care products are diversified, the consumption tends to be rational, and the potential of men skin care product consumers is very huge. At present, men's special cabinets are established for various international famous cosmetic brands such as Bieuquan, Qianbi, Diao, Irelaya and Zishengtang, the supplied products mainly relate to daily cleaning and nursing products such as shaving cream, aftershave lotion, facial cleanser, sunscreen cream and the like, while the functional skin care products such as emulsion, toner, facial cream, essence, facial mask and the like have fewer types and single effect, most of the functional skin care products only have the effects of controlling oil and moisturizing, but do not have the effects of resisting senility, removing wrinkles and the like, and are expensive.

Unlike the skin care products for women which emphasize whitening, tenderness and smoothness, skin care products for men have special needs. The horny layer of the skin of men is thicker than that of women, the number of skin glands is larger and more developed, and the sebaceous glands and sweat glands are larger than those of women, so that the skin absorption capacity of men is poorer than that of women, the skin oil is more, and 80% of men have oily skin or mixed oily skin. In addition, the pH value of the skin of the male is 4.5-6.0, the skin is lower than that of the female, and the acidity is higher than that of the female, so that the oil on the face is more, pores are easier to be large, and the pore blockage phenomenon is easier to cause acne and acne. In addition, because the stratum corneum is thicker in men's skin, once it is at an age where wrinkles are formed, it is generally deeper and wider than the wrinkles in women. Therefore, the development of corresponding skin care products aiming at the unique skin physiological characteristics of men is needed to meet the skin care requirements of men.

At present, the skin care products which are independently researched and developed aiming at the skin characteristics of men in China are fewer, the formula of the product can achieve various skin care effects by compounding plant extracts with different efficacies, the cost is higher, and the interaction among the components is more complex, for example, the Chinese patent application with the publication number of CN 108014045A, namely 'a skin care cosmetic composition for men and the application thereof', comprises the following components: the extract of the leaves of the sesamum indicum, the extract of the plum fruit, the extract of the medicinal phellinus igniarius, the extract of the leaves of the water shield, the extract of the leaves of the butterflybush flower, the extract of the laminaria saccharina, the tetrahydromethylpyrimidine carboxylic acid and the carnosine have various effects of efficiently preserving moisture, controlling oil, resisting aging, preventing skin sensitivity and inflammation and the like.

Disclosure of Invention

The invention aims to provide a male skin care composition containing growth factors, which is designed for male skin, has remarkable effects of moisturizing, controlling oil, resisting aging, removing wrinkles and the like, and can be applied to preparation of male skin care products such as toner, emulsion, cream, mask, essence and the like, so that the market of the male skin care products can be expanded, and the increasing male skin care requirements can be met.

The invention is realized by the following technical scheme:

a skin care composition containing growth factors for men comprises the following components in parts by weight: 0.00005-0.001 part of basic fibroblast growth factor, 3-9 parts of water-soluble fullerene, 1-6 parts of hydrolyzed rice protein and 0.5-3 parts of alpha-mannan.

Preferably, the skin care composition containing the growth factors for men comprises the following components in parts by weight: 0.0001-0.001 part of basic fibroblast growth factor, 4-8 parts of water-soluble fullerene, 2-5 parts of hydrolyzed rice protein and 1-3 parts of alpha-mannan.

Preferably, the skin care composition containing growth factors for men also comprises the following components in parts by weight: 1-8 parts of a Leucojum aestivum bulb extract and 1-5 parts of a bergamot extract.

More preferably, the skin care composition containing growth factors for men further comprises the following components in parts by weight: 0.0001-0.001 part of basic fibroblast growth factor, 5-8 parts of water-soluble fullerene, 2-5 parts of hydrolyzed rice protein, 1-3 parts of alpha-mannan, 3-6 parts of Leucojum aestivum bulb extract and 2-4 parts of bergamot extract.

Further, the water-solubleThe fullerene is keratan sulfate oligosaccharide-fullerene C₆₀A complex of fullerene C in said water-soluble fullerene₆₀The mass percentage of the catalyst is 4-8%.

Further, the keratan sulfate oligosaccharide is selected from at least one of keratan sulfate disaccharide and keratan sulfate trisaccharide. The keratan sulfate oligosaccharide can be a commercially available product or a self-made product, and the preparation method refers to Chinese patent application with publication number CN 1174557A, namely 'keratan sulfate oligosaccharide fraction and medicament containing the fraction', and specifically comprises the following steps: 50g of keratan sulfate was dissolved in 300ml of 0.1M acetic acid buffer (pH6.0), 25U of endo-. beta. -N-acetylglucosaminidase-type sulfate-degrading enzyme was added, and degradation was carried out at 37 ℃ for 24 hours. After the reaction, 2 times the volume of ethanol was added, stirred, left at room temperature for 1 night, and centrifuged at 4000rpm for 15min to obtain the supernatant (supernatant A). The precipitate was dissolved in 300ml of distilled water, and 3 times the amount of ethanol was added thereto, followed by stirring, standing at room temperature for 1 night, and centrifugation at 4000rpm for 15min to obtain a supernatant (supernatant B). Mixing the supernatant A and the supernatant B, concentrating under reduced pressure, performing Gel filtration by using a Bio-Gel-P-2 column (3.6 x 134cm) and distilled water as a solvent, freeze-drying, separating the prepared freeze-dried powder by using ion exchange chromatography, respectively collecting fractions of keratan sulfate disaccharide and keratan sulfate trisaccharide, and drying to obtain the final product.

Free radicals (active oxygen) are the fuse of the skin causing various skin problems such as wrinkles, darkness, color spots, dryness, aging, etc. Fullerene has super strong and persistent antioxidant capacity, can absorb and eliminate free radicals like sponge water absorption, thereby cutting off the source of skin problems, eliminating active oxygen generated under various conditions and having excellent skin care effect. However, fullerene is a football-like molecule consisting of 60 carbon atoms, and the carbon cage structure of fullerene has strong lipophilicity, so that the solubility of fullerene in polar solvents such as water is very limited, and the application of fullerene is limited. The invention fully combines fullerene and keratan sulfate oligosaccharide by a physical mixing method to prepare the keratan sulfate oligosaccharide-fullerene C₆₀The compound has better water solubility and good effects of resisting oxidation, inhibiting bacteria and softening cutin,specifically, the preparation method of the water-soluble fullerene comprises the following steps:

(1) reacting fullerene C₆₀Uniformly mixing the powder and keratan sulfate oligosaccharide at a weight ratio of 1: 20-50, grinding for 2-3 h at 600-800 r/min, adding double distilled water, placing in a constant temperature oscillator, and oscillating for 30min at 35-37 ℃ and a rotation speed of 100r/min to obtain a suspension;

(2) and (3) placing the suspension in an ultrasonic water bath kettle, treating for 60min under the conditions that the ultrasonic frequency is 20-30 kHz and the temperature is 60 ℃, then filtering by adopting a filter membrane with the diameter of 0.45 mu m, and concentrating and drying the filtrate to obtain the water-soluble fullerene.

The male skin care composition containing the growth factors can be used for preparing a male skin care product; the men's skin care product comprises, but is not limited to, toner, lotion, cream, mask or essence.

The male skin care composition containing the growth factors comprises the basic fibroblast growth factors, the water-soluble fullerene, the hydrolyzed rice protein, the alpha-mannan, the Leucojum aestivum bulb extract and the bergamot extract, and the components are reasonably compounded, so that the male skin care composition has an obvious synergistic effect, and can achieve various skin care effects of moisturizing, oil control, anti-aging, wrinkle removal and the like. The basic fibroblast growth factor can improve the microenvironment for cell growth, promote the growth and development of fibroblasts and the synthesis of elastic fibers and collagen, has the effects of removing and preventing wrinkles, and has very weak effects of moisturizing, controlling oil and removing free radicals. The invention is further compounded with keratan sulfate oligosaccharide-fullerene C with better water solubility₆₀The compound can obtain better oil control and oxidation resistance effects, and utilizes fullerene C₆₀Softening cutin, making the basic fibroblast growth factor be absorbed by skin more easily and fully. In addition, the alpha-mannan molecule contains a large number of polar groups such as hydroxyl, carboxyl and the like, can combine a large number of water, enables the molecules to be mutually interwoven into a net shape, has strong water-locking and moisture-preserving performance, and plays a role in high-efficiency moisture preservation and skin care.

However, the present inventors have found in their studies thatDue to the fact that the keratan sulfate oligosaccharide-fullerene C₆₀The complex incorporates a keratan sulfate disaccharide and/or a keratan sulfate trisaccharide of relatively low molecular weight, relative to the fullerene C₆₀The content of the keratan sulfate disaccharide and/or the keratan sulfate trisaccharide is high, and after long-term use, the risk of skin saccharification is caused, so that the skin premature senility is possibly responded to the certain extent; the hydrolyzed rice protein can activate proteasome activity to convert damaged proteins into healthy proteins; the Leucojum aestivum bulb extract can enhance the activity of lysosomes, promote the degradation of damaged proteins, effectively prevent the formation of a saccharification end product, reduce the skin saccharification risk of keratan sulfate disaccharide and keratan sulfate trisaccharide, and inhibit the saccharification effect of free sugar contained in the skin, thereby fully playing the anti-aging effect.

Compared with the prior art, the invention has the following beneficial effects:

(1) the skin care composition provided by the invention is designed aiming at the unique skin types of men, has excellent effects of moisturizing, controlling oil, resisting allergy, removing wrinkles, resisting oxidation, resisting aging and the like, and strives to ensure that the effective ingredients are in close contact with the skin, thereby achieving the double effects of beautifying the external skin and regulating the internal organs and meeting the common requirements of different skin forms.

(2) The skin care composition provided by the invention has the advantages of reasonable component matching, scientific proportioning, synergistic interaction, mild property, safety and effectiveness, can be prepared into various skin care products for men, can meet the increasing skin care requirements for men, and can expand the market of the skin care products for men.

Detailed Description

The following examples are further illustrative of the present invention and are not intended to be limiting thereof. The components of the formulations in the following examples are, unless otherwise specified, conventionalCommercially available products, of which hydrolyzed rice protein (Prolixir ICE) was purchased from Ashland, usa, Ashland group; leucojum aestivum bulb extract

From Israel IBR, bergamot extract from orange Ti Tech (Active concentrates) USA.

Example 1 preparation of Water-soluble Fullerene

(1) 10g of keratan sulfate disaccharide and 500mg of fullerene C were weighed₆₀Mixing the powders, grinding at 600r/min for 3h, adding double distilled water, placing in a constant temperature oscillator, and oscillating at 35 deg.C and 100r/min for 30min to obtain suspension;

(2) placing the suspension in ultrasonic water bath, treating at ultrasonic frequency of 30kHz and temperature of 60 deg.C for 60min, filtering with 0.45 μm filter membrane, concentrating the filtrate, and drying to obtain keratan sulfate disaccharide-fullerene C₆₀A complex of fullerene C₆₀The mass percentage of the composite material is 8 percent.

Example 2 preparation of Water-soluble Fullerene

(1) 10g of keratan sulfate trisaccharide and 200mg of fullerene C were weighed₆₀Mixing the powders, grinding at 800r/min for 2h, adding double distilled water, placing in a constant temperature oscillator, and oscillating at 37 deg.C and 100r/min for 30min to obtain suspension;

(2) placing the suspension in ultrasonic water bath, treating at ultrasonic frequency of 20kHz and temperature of 60 deg.C for 60min, filtering with 0.45 μm filter membrane, concentrating the filtrate, and drying to obtain keratan sulfate trisaccharide-fullerene C₆₀A complex of fullerene C₆₀The mass percentage of the catalyst is 4 percent.

Examples 3-8 preparation of Male skin-care composition

Examples 3-8 preparation of a men's skin care composition comprising the following components in parts by weight:

the preparation method comprises the following steps: the raw materials of the components of the formula are respectively weighed according to the weight parts shown in the table and are uniformly mixed to obtain the skin care composition for men.

Comparative example 1 preparation of skin care composition for men

Comparative example 1 a skin care composition for men was prepared comprising the following components in parts by weight: basic fibroblast growth factor 0.0005 parts, hydrolyzed rice protein 3 parts, and alpha-mannan 2 parts, as in examples 3-8 above.

Comparative example 2 preparation of skin care composition for men

Comparative example 2 a skin care composition for men was prepared comprising the following components in parts by weight: basic fibroblast growth factor 0.0005 parts, hydrolyzed rice protein 3 parts, alpha-mannan 2 parts, Leucojum aestivum bulb extract 5 parts, and bergamot extract 3 parts, prepared as in examples 3-8 above.

Essence containing the men's skin care composition of example 3 was prepared:

(1) the formula is as follows:

(2) the preparation method comprises the following steps: adding the male skin care composition, edetate disodium and glycerin obtained in the example 3 into deionized water, heating to 37 ℃, stirring and mixing uniformly, wherein the stirring speed is 100 revolutions per minute; and (3) after uniformly mixing, cooling to 28 ℃, adding the acryloyl dimethyl ammonium taurate/VP copolymer and the ethylhexyl glycerol, uniformly mixing, and subpackaging to obtain the essence.

Preparation of essence containing the men's skin care compositions of examples 4 to 8 and comparative examples 1 to 2 referring to the above, the skin care composition for men was added in an amount of 10 wt% in each test example.

Skin lotion containing the male skin care composition of example 3 was prepared:

(1) the formula is as follows:

example 3 skin care composition for men 6 wt%

4 wt% of glycerin

0.2 wt% of phenoxyethanol

The balance being deionized water.

(2) The preparation method comprises the following steps: adding the male skin care composition, glycerol and phenoxyethanol in the deionized water, stirring until the materials are completely dissolved, and subpackaging to obtain the skin care lotion.

Skin lotion preparation containing the men's skin care compositions of examples 4 to 8 and comparative examples 1 to 2 referring to the above, the corresponding men's skin care composition was added in an amount of 6 wt% in each test example.

Test example I, skin irritation test

160 test volunteers were selected as subjects and divided into 8 groups of examples 3-8 and comparative examples 1-2, and 20 subjects were administered with skin lotions containing the men's skin care compositions of examples 3-8 and comparative examples 1-2. 0.03mL of the sample was taken by a syringe and placed in a chamber of a spot test tape, and the spot test tape with the sample was immediately stuck to the normal skin of the left forearm from the lower part in the longitudinal direction while gently pressing the chamber one by one to expel air and uniformly distribute the test substance. The test site is marked for easy observation. The patch was applied every 24 hours and then removed for a half hour, and the patch was examined in accordance with the CTFA guideline terminology for 6 patch tests, and on the 6 th day after the completion of the patch, additional examination was performed to observe delayed reactions. The results of the last examination are compared and the criteria for identification are shown in table 1 and the results are shown in table 2.

TABLE 1 identification standards

Grade	Symbol	Identification criteria
			0	-	Negative reaction; has no irritation and erythema
1	±	And (3) suspicious reaction: mild erythema
			2	+	Weak positive reaction: erythema
3	++	Strong positive reaction: erythema, papules, blisters
			4	+++	Extremely strong positive reaction: severe edema and bulla

Table 2 skin irritation test results

The results show that the skin care lotion containing the male skin care compositions of examples 3-8 and comparative examples 1-2 has no positive reaction, and the skin irritation grade is 0, which indicates that the male skin care composition provided by the invention has no irritation to skin, and the product is safe and mild.

Test example two, moisturizing effect test

200 test volunteers were selected as subjects, and divided into 8 groups of examples 3-8 and comparative examples 1-2, and 25 subjects were administered with essence containing the men's skin care composition according to examples 3-8 and comparative examples 1-2. The essence is required to be applied to a test volunteer once a day in the morning and evening by taking a proper amount of the essence and lightly smearing the essence on the face, and then lightly tapping the face until the skin is completely absorbed. Each user was followed up periodically using a skin tester (Derma-Expert MC760) with a phase of testing every 10 days, with both cheeks on the test site. The moisture content of the skin was tested and the results are shown in table 3 below.

Table 3 evaluation results of the skin moisture content increase of the essence containing the skin care composition for men according to the present invention

Note: d₀Data measured for day 0, D₁₀Data measured on day 10, D₂₀Data measured on day 20.

The results show that the essence containing the men's skin care composition can remarkably improve the moisture content of the skin, and the men's skin care composition has good moisturizing effect and the best effect as shown in example 8.

Test example three, oil control effect test

160 male test volunteers were selected as test subjects, all had oily and mixed skin, had no skin disease or skin allergy history, and were divided into 8 groups, namely, examples 3 to 8 groups and comparative examples 1 to 2 groups, and 20 male skin care compositions of examples 3 to 8 and comparative examples 1 to 2 were administered to the test subjects. The test samples were administered to the volunteers in the morning and evening daily for 4 weeks. Before and after 4 weeks of use, the skin oil content of the tested volunteers was tested according to the world recognized SEUMETER method by using a skin oil test probe (Sebumeter, Germany CK), and during the test, the forehead part was tested 3 times by using the skin oil test probe, and the average value was obtained, and the test results are shown in Table 4.

TABLE 4 evaluation of oil control Effect of skin moisturizer containing the skin care composition for men of the present invention

Group of	Before use	After 4 weeks of use	Oil Change Rate (%)
				Example 3	120.32±30.45	89.23±22.05	-25.84
Example 4	123.17±33.08	96.91±26.12	-21.32
				Example 5	124.50±32.26	95.03±25.20	-23.67
Example 6	118.92±29.94	84.97±20.74	-28.55
				Example 7	117.67±29.42	86.02±21.48	-26.90
Example 8	119.54±30.05	82.77±23.17	-30.76
				Comparative example 1	120.06±31.36	102.84±28.72	-14.34
Comparative example 2	119.88±28.72	93.29±26.58	-22.18

The results show that the skin lotion containing the male skin care composition can effectively reduce the secretion of skin grease, achieves good oil control effect, and shows that the male skin care composition has good oil control effect, and the effect is best as shown in example 8.

Test example four anti-wrinkle Effect test

200 test volunteers were selected as subjects, and divided into 8 groups of examples 3-8 and comparative examples 1-2, and 25 subjects were administered with essence containing the men's skin care composition according to examples 3-8 and comparative examples 1-2. The application method comprises applying appropriate amount of the extract on face, and slightly looping until the essence is completely absorbed. Each user was followed up periodically, once a week for a phase of testing for a total of four weeks, with the test sites being the cheeks and canthus on both sides. Using MicroSkinII MutifunctionalCan be measured by a skin mirror image analysis system. The value of each measurement of the experimental part of the tested volunteer is counted, and the skin texture is analyzed according to the average roughness and the average depth of wrinkles of the skin. Wherein, the average roughness of the skin refers to the average value of the difference between the maximum gray peak value and the minimum gray peak value on a straight line in all the sections of the skin; the average wrinkle depth is the average distance between the peaks of the target skin dome (maximum gray scale curve) and the lowest point of the skin furrow (minimum gray scale curve). And the following formula is used for calculation: average skin roughness change rate (sample group data T)_n-a start value T₀) Initial value T₀X 100% and wrinkle average depth change rate (sample group data T)_n-a start value T₀) Initial value T₀×100％，T₀Is the skin measurement value of the tested volunteer before the test, T_nIs the skin measurement value of each test volunteer at each time point. The results of the experiments are shown in tables 5 and 6.

TABLE 5 difference between average roughness of skin at the test site and the initial value

The results showed that the skin roughness average of the skin of the test site using the essence for skin care composition for men of the present invention was always in a decreasing trend within 4 weeks of the test period, and the change rate of skin roughness average was 6.9% after 4 weeks as the best effect of example 8.

TABLE 6 difference between mean wrinkle depth and initial value at test site

The results showed that the average wrinkle depth of the skin care composition essence for men of the present invention was decreased at 4 weeks in the test period, and the effect was the best as in example 8, and the change rate of the average wrinkle depth was 8.2% after 4 weeks.

The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.

Claims

1. The skin care composition containing growth factors for men is characterized by comprising the following components in parts by weight: 0.00005-0.001 part of basic fibroblast growth factor, 3-9 parts of water-soluble fullerene, 1-6 parts of hydrolyzed rice protein and 0.5-3 parts of alpha-mannan;

the water-soluble fullerene is keratan sulfate oligosaccharide-fullerene C₆₀A complex of fullerene C in said water-soluble fullerene₆₀The mass percentage of the catalyst is 4-8%.

2. The skin care composition containing growth factors for men according to claim 1, which is characterized by comprising the following components in parts by weight: 0.0001-0.001 part of basic fibroblast growth factor, 4-8 parts of water-soluble fullerene, 2-5 parts of hydrolyzed rice protein and 1-3 parts of alpha-mannan.

3. The skin care composition containing growth factors for men according to claim 1 or 2, further comprising the following components in parts by weight: 1-8 parts of a Leucojum aestivum bulb extract and 1-5 parts of a bergamot extract.

4. The skin care composition containing growth factors for men according to claim 3, which is characterized by comprising the following components in parts by weight: 0.0001-0.001 part of basic fibroblast growth factor, 5-8 parts of water-soluble fullerene, 2-5 parts of hydrolyzed rice protein, 1-3 parts of alpha-mannan, 3-6 parts of Leucojum aestivum bulb extract and 2-4 parts of bergamot extract.

5. The male skin care composition containing growth factors according to claim 1, wherein the keratin sulfate oligosaccharides are selected from at least one of keratin sulfate disaccharides and keratin sulfate trisaccharides.

6. The growth factor-containing men's skin care composition according to claim 1, wherein the preparation of the water-soluble fullerene comprises the following steps:

7. Use of the growth factor-containing men's skin care composition according to any one of claims 1 to 6 for preparing a men's skin care product.

8. A skin care product for men, which is characterized by comprising the skin care composition containing growth factors for men, according to any one of claims 1 to 6.

9. The male skin care product according to claim 8, wherein the male skin care product is a toner, a lotion, a cream, a mask or an essence.