CN115006313B

CN115006313B - Fullerene-containing whitening and moisturizing gel mask and preparation method thereof

Info

Publication number: CN115006313B
Application number: CN202210811428.9A
Authority: CN
Inventors: 张振芳
Original assignee: Beijing Rich Lasy Science And Technology Co ltd
Current assignee: Beijing Rich Lasy Science And Technology Co ltd
Priority date: 2022-07-11
Filing date: 2022-07-11
Publication date: 2023-08-22
Anticipated expiration: 2042-07-11
Also published as: CN115006313A

Abstract

The invention discloses a whitening and moisturizing gel mask containing fullerene and a preparation method thereof, and relates to the technical field of cosmetic preparation, wherein the gel mask comprises the following components: water-soluble fullerene, white firewood extract, ceramide, carbomer 940, sodium bicarbonate, glycerol, peregal O and deionized water. Through the synergistic effect of a plurality of components, the whitening effect is very good.

Description

Fullerene-containing whitening and moisturizing gel mask and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetic preparation, in particular to a whitening and moisturizing gel mask containing fullerene and a preparation method thereof.

Background

With the development of society, the living standard of people is improved, the requirements of people on cosmetics are also becoming higher, and the care of skin of people who love beauty is very important, and meanwhile, the efficacy and the safety of people are also very important. However, in life, dust, dirty air, ultraviolet rays in sunlight and computer radiation, and daily makeup of people with light illumination are all major enemies for skin care, so that the aging of the skin is quickened, and the skin color is dark yellow. However, the skin is maintained regularly, so that the aging of the skin can be effectively delayed, and the effect of skin health and brightening is achieved.

The types and kinds of facial masks on the market at present are various, but the most main types are still whitening facial masks. In the prior art, the most widely used whitening agents are vitamins such as nicotinamide, tranexamic acid, arbutin, resorcinol and derivatives thereof. They generally achieve whitening effects by inhibiting melanocyte proliferation, inhibiting tyrosinase and other Guan Xiansu enzyme activities, and inhibiting melanin transfer. However, the whitening mechanism of fullerenes is different from that of the conventional one by inhibiting tyrosinase activity, and is mainly achieved by scavenging free radicals and inhibiting cytokines. According to the research, the fullerene can improve melanin generation caused by UVA, and has obvious effect and even stronger effect than VC and arbutin.

However, we have found through research that, in the existing whitening mask, including other products such as face cream, the effect of fullerene is mainly represented in terms of moisture retention and wrinkle resistance, and the whitening effect of the product is not significantly affected by adding the component or not, or the component is synergistic with some other known whitening components, so that the whitening effect of the component is enhanced, and the enhancement effect is not significant.

Disclosure of Invention

In order to solve the problems of the prior mask, the invention provides a whitening and moisturizing gel mask containing fullerene and a preparation method thereof, and the specific scheme is as follows:

a fullerene-containing whitening and moisturizing gel mask comprising: water-soluble fullerene, white firewood extract, ceramide, carbomer 940, sodium bicarbonate, glycerol, peregal O and deionized water.

Preferably, the whitening and moisturizing gel mask containing fullerene comprises, by mass, 4-8 parts of water-soluble fullerene, 13-22 parts of white firewood extract, 0.5-2 parts of ceramide and 940 parts of carbomer: 3-6 parts of sodium bicarbonate, 0.3-3 parts of sodium bicarbonate, 2-10 parts of glycerol, 0.5-2 parts of peregal O and 25-70 parts of deionized water.

Preferably, the whitening and moisturizing gel mask containing fullerene further comprises magnesium sulfate and sodium chloride.

Preferably, the weight parts of magnesium sulfate and sodium chloride in the fullerene-containing whitening and moisturizing gel mask are respectively 0.02-0.1 part and 0.02-0.05 part.

Preferably, the whitening and moisturizing gel mask containing fullerene further comprises provitamin B5, imidazolidinyl urea and sorbic acid.

Preferably, the vitamin B5, the imidazolidinyl urea and the sorbic acid in the fullerene-containing whitening and moisturizing gel mask are respectively 0.5-1 part, 0.3-0.6 part and 0.1-0.4 part by mass.

Preferably, the whitening and moisturizing gel mask containing fullerene also comprises 0.5-2 parts by weight of tween-80 and 0.5-2 parts by weight of propylene glycol.

Preferably, the whitening and moisturizing gel mask containing fullerene further comprises 0.1-0.2 part by weight of hypromellose.

The preparation method of the firewood extract comprises the following steps: the method for extracting the firewood extract comprises the following steps: pulverizing dried radix Cynanchi Stauntonii, sieving with 100 mesh sieve, adding the obtained powder into 15-30 times of 70% ethanol water solution with pH of 7.0-7.5, reflux extracting at 85-90deg.C for 50-100 min, distilling the obtained extractive solution under reduced pressure, concentrating, and lyophilizing to obtain powdery radix Cynanchi Stauntonii extract.

The invention also provides a preparation method of the fullerene-containing whitening and moisturizing gel mask, which comprises the following steps:

s1, slowly stirring and mixing water-soluble fullerene, a white firewood extract, ceramide, carbomer 940 and peregal O to obtain a mixture a;

s2, slowly adding the mixture a into glycerol under stirring to obtain a mixture b;

s3, dissolving sodium bicarbonate in deionized water to obtain a solution c;

and S4, adding the solution c into the mixture b, and stirring and mixing uniformly to obtain the whitening and moisturizing gel mask containing fullerene.

Preferably, when magnesium sulfate is further included in the fullerene-containing whitening and moisturizing gel mask, magnesium sulfate is added to the mixture a before S2.

Preferably, when the fullerene-containing whitening and moisturizing gel mask further comprises provitamin B5, imidazolidinyl urea and sorbic acid, the provitamin B5, the imidazolidinyl urea and the sorbic acid are added into the mixture a before S2.

Preferably, when tween-80 and propylene glycol are further included in the fullerene-containing whitening and moisturizing gel mask, tween-80 and propylene glycol are added to the mixture a before S2.

Preferably, when the fullerene-containing whitening and moisturizing gel mask further comprises hypromellose, the fullerene-containing whitening and moisturizing gel mask is added to the mixture a before S2.

Advantageous effects

The invention has the beneficial effects that:

1. the water-soluble fullerene can be better fused with various water-soluble components commonly used in cosmetics, is convenient for being released from the facial mask and fully plays a role, further improves the capability of scavenging free radicals, and has better whitening, moisturizing and anti-aging effects. The vitamin B5 has the moisture-preserving effect of penetrating, is easily received and absorbed by skin, can infiltrate into stratum corneum after being used, is very suitable for skin with poor absorption, and the moisture-preserving effect is achieved by increasing the hyaluronic acid content of the skin. Besides moisturizing, the vitamin B5 is also a repairing component, and is beneficial to regeneration of skin cells while repairing skin micro-damage. Ceramide is an ingredient originally existing in skin, and is intercellular lipid which is formed by combining cholesterol and unsaturated fatty acid. The composition has the moisturizing effect, and can help skin to retain moisture for a long time. However, if a large amount of water is lost, the original moisture of the skin can be lost very quickly and easily falls into a dry itching and allergy state, and the moisturizing effect is remarkable when the ceramide is used for moisturizing.

2. The Bai Hua Cai is Tirpitzia ovoidea Chun et How of Linaceae, and is prepared from branches, stems and leaves. In the prior researches or applications, the main functions are as follows: promoting blood circulation, removing blood stasis, relaxing tendons and activating collaterals. Traumatic injury, fracture: mashing fresh leaves, parching with wine, and applying externally. Traumatic hemorrhage: the fresh leaves are mashed and externally applied. Rheumatic arthritis, sequelae of poliomyelitis: the preparation is administered by decocting with 3-5 times of branches, stems and leaves with water or stewing pig bones. Sore and furuncle: proper amount of fresh leaves are decocted with water and washed. The research of the invention discovers that the extract can effectively inhibit tyrosinase activity when being externally applied to the skin surface, has good whitening effect and acne removing effect, and the whitening effect is greatly improved through the synergistic effect with fullerene.

3. The firewood extract is used together with magnesium sulfate and sodium chloride, so that in the past work, the magnesium sulfate and sodium chloride are often used in systems such as foundation liquid and the like in application and cosmetics, and are used for providing ionic property, improving charge, stabilizing the systems and the like; the magnesium sulfate can be used as a thickener and mineral salt in cosmetics, has the effects of diminishing inflammation, removing swelling and the like, and can reduce the damage of local tissues; however, in the invention, the magnesium sulfate and the sodium chloride do not need to provide the ionization, and the effect of the magnesium sulfate and the sodium chloride in the invention is to improve the tyrosinase activity inhibition rate of the firewood extract and improve the whitening effect of the firewood extract; however, when the addition amount of the two is respectively more than 0.1 part of magnesium sulfate or 0.05 part of sodium chloride, the gel effect of the mask is reduced: is not easy to gel or has too low film forming strength.

4. In the research of the invention, the strength of the gel mask is obviously reduced after the firewood extract (aqueous solution) is added in the mask formula, and possibly, certain substances in the gel mask cause the gel to be difficult to form complexation, but the problem is solved after the hypromellose is added.

5. According to the research of the invention, after the firewood extract (aqueous solution) is added into the mask formula, the water locking capacity of the gel mask is reduced, the water retention time of the mask is shortened, the gel mask cannot be used continuously until the release of active ingredients is complete (the gel mask is not suitable for being used after the water content is lower than 40% wt, and has adverse effects on skin), and the problem is solved after the Tween-80 and the propylene glycol are added and the specific ratio of the gel mask to the gel key components of the mask is set, so that the requirement of the daily mask on the use time (25-30 min) can be met.

Detailed Description

Other advantages and effects of the present invention will become apparent to those skilled in the art from the following disclosure, which describes the embodiments of the present invention with reference to specific examples. The invention may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present invention.

Unless otherwise indicated, the following examples and comparative examples were parallel runs, using the same processing steps and parameters.

The embodiment provides a whitening and moisturizing gel mask containing fullerene, which comprises the following components: water-soluble fullerene, white firewood extract, ceramide, carbomer 940, sodium bicarbonate, glycerol, peregal O and deionized water.

The whitening and moisturizing gel mask containing the fullerene comprises, by mass, 4-8 parts of water-soluble fullerene (fullerene for short), 13-22 parts of firewood extract (extract for short), 0.5-2 parts of ceramide (amide for short) and carbomer 940 (carbomer for short): 3-6 parts of sodium bicarbonate (sodium bicarbonate for short), 0.3-3 parts of glycerin, 2-10 parts of peregal O (peregal for short), 0.5-2 parts of deionized water (water for short) and 25-70 parts of deionized water.

The whitening and moisturizing gel mask containing the fullerene also comprises magnesium sulfate and sodium chloride.

The weight portions of the magnesium sulfate and the sodium chloride in the whitening and moisturizing gel mask containing the fullerene are respectively 0.02-0.1 part and 0.02-0.05 part.

The whitening and moisturizing gel mask containing fullerene also comprises provitamin B5 (for short, vitamin), imidazolidinyl urea (for short, urea) and sorbic acid.

The weight parts of the vitamin B5, the imidazolidinyl urea and the sorbic acid in the fullerene-containing whitening and moisturizing gel mask are respectively 0.5-1 part, 0.3-0.6 part and 0.1-0.4 part.

The whitening and moisturizing gel mask containing the fullerene also comprises 0.5-2 parts by weight of tween-80 (Tween for short) and 0.5-2 parts by weight of propylene glycol.

The whitening and moisturizing gel mask containing the fullerene also comprises 0.1-0.2 part by weight of hydroxypropyl methylcellulose (called cellulose for short).

The preparation method of the firewood extract-a comprises the following steps: the method for extracting the firewood extract comprises the following steps: pulverizing dried radix Cynanchi Stauntonii, sieving with 100 mesh sieve, adding the obtained powder into 20 times of 70% ethanol water solution with pH of 7.0, reflux-extracting at 55-65deg.C for 80 min, distilling the obtained extractive solution under reduced pressure, concentrating, and lyophilizing to obtain powdery radix Cynanchi Stauntonii extract.

Preparation method of the firewood extract-b (for comparison): the method for extracting the firewood extract comprises the following steps: pulverizing dried radix Cynanchi Stauntonii, sieving with 100 mesh sieve, adding the obtained powder into 70% ethanol water solution with pH of 7.0, heating and reflux extracting at 70-80deg.C for 80 min, distilling the obtained extractive solution under reduced pressure, concentrating, and lyophilizing to obtain powdery radix Cynanchi Stauntonii extract.

s3, dissolving sodium bicarbonate in deionized water to obtain a solution c;

When the fullerene-containing whitening and moisturizing gel mask further comprises magnesium sulfate, the magnesium sulfate is added into the mixture a before S2.

When the fullerene-containing whitening and moisturizing gel mask further comprises vitamin B5, imidazolidinyl urea and sorbic acid, the vitamin B5, the imidazolidinyl urea and the sorbic acid are added into the mixture a before S2.

When tween-80 and propylene glycol are further included in the fullerene-containing whitening and moisturizing gel mask, tween-80 and propylene glycol are added to the mixture a before S2.

When the fullerene-containing whitening and moisturizing gel mask further comprises hypromellose, the fullerene-containing whitening and moisturizing gel mask is added into the mixture a before S2.

TABLE 1 example 1-x Components Table

The extract of the firewood alba in the examples in the table adopts the firewood alba extract-a.

Example 7: the difference from example 1 is that: the radix Cynanchi auriculati extract is radix Cynanchi auriculati extract-b, and others.

Comparative example 0: the difference from example 1 is that: contains no fullerene and no extract.

Examples 3-3: the difference from example 3-2 is that: does not contain fullerene.

And (3) detection:

1. the erythrocyte hemolysis test was performed according to known methods, and all of the above samples were non-irritating according to current standards.

2. Tyrosinase activity inhibition assay: mouse melanoma B16 cells in logarithmic growth phase were inoculated into 6-well cell culture plates and cultured overnight. Test samples with a final volume fraction of 1% were added separately, and the untreated group was used as a cell control group, with 2 duplicate wells per group. After 48h incubation, washing 1 with PBS, adding 100. Mu.L of lysate to each well, scraping the collected cells, and centrifuging to collect the supernatant. mu.L of the cell supernatant was taken into a 96-well plate, 50. Mu.L of 1% L-dopa solution was added thereto, incubated at 37℃for 1 hour, and absorbance was read at 475nm by an M3 plate reader.

Relative tyrosinase activity (%) = (assay well absorbance value-blank absorbance value)/(cell control absorbance value-blank absorbance value) ×100%.

3. Melanin synthesis inhibition experiments: taking mouse melanoma B16 cells in logarithmic growth phase, inoculating the cells into a T25 cell culture flask, and culturing overnight. Test samples were added in 1% final volume fractions, respectively, and the untreated group was used as a cell control group. After 48h incubation, washing 1 time with PBS, adding 1mL of 1mol/LNaOH solution, scraping the collected cells, placing in a 80℃water bath for 30min, taking the supernatant, adding into a 96-well plate, and reading the absorbance at 475nm by an M3 plate reader.

Relative melanin content (%) = (measured well absorbance value-blank absorbance value)/(cell control absorbance value-blank absorbance value) ×100%. The tyrosinase activity and melanin synthesis inhibition test results are shown in Table 2, wherein the tyrosinase activity of the control group is 100%, and the melanin content of the control group is 100%.

TABLE 2 sample tyrosinase activity and melanin synthesis inhibition assay results

As is clear from comparative example 0, example 1-1 and example 1-2, the pure addition of fullerene has no effect on whitening, and no synergistic effect exists between fullerene and other components except the extract, so that the whitening effect can be affected; the extract has skin whitening effect. By combining the examples 1 and 2, the addition of fullerene and the extract produce synergistic effect, thus greatly improving the whitening effect. In combination with example 3, example 3-1 and example 3-2, it is known that the addition of magnesium sulfate and sodium chloride can form complex synergistic effect with fullerene and extract to further enhance whitening effect, but magnesium sulfate and sodium chloride cannot cooperate with extract on the premise that fullerene is not present, and cannot cooperate with fullerene on the premise that extract is not present, and example 3-3 shows that magnesium sulfate and sodium chloride are added to the mask itself without whitening effect.

From other characteristics of the mask, the non-sleeping effect of the mask of the embodiment 4 is better, the water locking capability of the mask of the embodiment 5 is higher, the requirement of the daily mask on the service time (25-30 min) can be met, the mask of the embodiment 6 is higher in strength, and the whitening effect of the three is basically the same as that of the embodiment 1.

4. Human body evaluation of whitening effect

120 volunteers with whitening requirements between 30 and 40 years old were selected as subjects and divided into 12 groups, and the masks prepared by using all 11 examples and comparative example 0 were used three times a week for 4 weeks (using time 20±2min each time).

The skin melanin content changes (averaged over ten persons per group) of the subjects before and after application of the mask composition were evaluated using a skin red melanin tester (Hexameter MX 18). The measurement range of the instrument used is 0-999, the higher the measurement value, the higher the melanin content in the skin. The statistical results of melanin content of the front and rear facial skin of the subject using the mask composition prepared in the present invention are shown in table 3.

TABLE 3 skin melanin content statistics

Facial mask	Before use	Used for 1 week	For 2 weeks	Used for 4 weeks
					Example 1	232.8	217.7	200.3	178.8
Example 2	237.1	223.5	211.0	187.3
					Example 1-1	241.0	236.6	228.9	218.8
Examples 1 to 2	237.7	235.1	231.9	225.1
					Comparative example 0	249.1	244.1	238.8	231.9
Example 3	241.1	220.9	196.3	150.2
					Example 4	246.3	223.3	198.7	165.8
Example 5	235.8	217.9	196.1	163.2
					Example 6	235.7	219.0	200.1	179.8
Example 3-1	241.9	235.2	228.6	215.3
					Example 3-2	239.3	232.8	227.1	222.6
Examples 3 to 3	238.7	231.0	225.6	221.9
					Example 7	244.0	239.9	235.1	229.4

As shown in Table 3, the whitening effect of the facial mask provided by the invention is better, and the melanin content in the skin can be obviously reduced after 4 weeks of use, and the reduction of melanin can reach 32.7% at most (example 4). From the rule of table 3, the mask is similar to the test rule of table 2 in practical application, except that in practical application, it is obvious that the components added in examples 4 and 5 are more beneficial to the whitening components in the mask to play a role, so that a better whitening effect is obtained.

While the preferred embodiments and examples of the present invention have been described in detail, the present invention is not limited to the above-described embodiments and examples, and various changes may be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.

Claims

1. A whitening and moisturizing gel mask containing fullerene is characterized in that: comprising the following steps: water-soluble fullerene, white firewood extract, ceramide, carbomer 940, sodium bicarbonate, glycerol, peregal O and deionized water;

the components comprise 4-8 parts of water-soluble fullerene, 13-22 parts of white firewood extract, 0.5-2 parts of ceramide and carbomer 940:3-6 parts of sodium bicarbonate, 0.3-3 parts of sodium bicarbonate, 2-10 parts of glycerol, 0.5-2 parts of peregal O and 25-70 parts of deionized water;

the method for extracting the firewood extract comprises the following steps: pulverizing dried radix Cynanchi Stauntonii, sieving with 100 mesh sieve, adding the obtained powder into 15-30 times of 70% ethanol water solution with pH of 7.0-7.5, reflux extracting at 85-90deg.C for 50-100 min, distilling the obtained extractive solution under reduced pressure, concentrating, and lyophilizing to obtain powdery radix Cynanchi Stauntonii extract.

2. The fullerene-containing whitening and moisturizing gel mask of claim 1, wherein: the whitening and moisturizing gel mask containing the fullerene also comprises magnesium sulfate and sodium chloride.

3. The fullerene-containing whitening and moisturizing gel mask of claim 2, wherein: the weight portions of magnesium sulfate and sodium chloride in the fullerene-containing whitening and moisturizing gel mask are respectively 0.02-0.1 part and 0.02-0.05 part.

4. The fullerene-containing whitening and moisturizing gel mask of claim 1, wherein: the whitening and moisturizing gel mask containing fullerene also comprises vitamin B5, imidazolidinyl urea and sorbic acid.

5. The fullerene containing whitening and moisturizing gel mask of claim 4, wherein: the whitening and moisturizing gel mask containing the fullerene comprises, by mass, 0.5-1 part of vitamin B5, 0.3-0.6 part of imidazolidinyl urea and 0.1-0.4 part of sorbic acid.

6. The fullerene-containing whitening and moisturizing gel mask of claim 1, wherein: the whitening and moisturizing gel mask containing the fullerene also comprises 0.5-2 parts by weight of tween-80 and 0.5-2 parts by weight of propylene glycol.

7. The fullerene-containing whitening and moisturizing gel mask of claim 1, wherein: the whitening and moisturizing gel mask containing the fullerene also comprises 0.1-0.2 part by weight of hydroxypropyl methylcellulose.

8. A method for preparing the fullerene-containing whitening and moisturizing gel mask according to any one of claims 1 to 7, which is characterized in that: the method comprises the following steps:

s3, dissolving sodium bicarbonate in deionized water to obtain a solution c;