CN111939097A

CN111939097A - Moisturizing and allergy-relieving composition and application thereof

Info

Publication number: CN111939097A
Application number: CN202010866328.7A
Authority: CN
Inventors: 夏洁; 蒙宇; 李春煌; 林进光; 胡根华; 吴知情
Original assignee: Guangzhou Nabion Biotechnology Co ltd
Current assignee: Guangzhou Nabion Biotechnology Co ltd
Priority date: 2020-08-25
Filing date: 2020-08-25
Publication date: 2020-11-17

Abstract

The invention provides a moisturizing and allergy-relieving composition and application thereof, wherein the composition comprises white pear juice, lucid ganoderma extract, honeysuckle extract, forsythia extract, amino acid and aloe leaf water; the preparation method of the composition comprises the following steps: adding white pear juice, Ganoderma extract, flos Lonicerae extract, fructus forsythiae extract and amino acids into folium Aloe water at a certain proportion, and mixing well. The components of the moisturizing and allergy-relieving composition provided by the invention are synergistic, so that the moisturizing and allergy-relieving effects are achieved from multiple aspects of enhancing the barrier function of skin, increasing the water content of stratum corneum, repairing inflammatory reaction and the like, the composition has a targeted adjusting effect on dry and sensitive skin, and can be used for preparing moisturizing and allergy-relieving cosmetics.

Description

Moisturizing and allergy-relieving composition and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and relates to a moisturizing and allergy-relieving composition and application thereof.

Background

When the external environment changes and the metabolism in the human body is slow, the skin is extremely dry, which means that the skin barrier is disordered, the moisture content of the stratum corneum is reduced, and local inflammatory factors (such as IL-1 and TNF) are released. Sensitive skin refers to a high-response state of the skin under physiological or pathological conditions, and may show subjective symptoms such as burning, itching, stabbing pain and tightness, with or without objective signs such as erythema, desquamation and telangiectasia. Studies have shown that a decrease in the barrier function of the skin and the presence of inflammatory reactions in the top layer of the skin are important factors leading to problems of dryness and sensitivity of the skin. Skin is an important tissue which shows the external beauty and characteristics of human body, so that the problems of dry skin, sensitivity and the like which influence the health of skin gradually draw attention to people.

At present, one of the common means for improving skin condition is to use moisturizing and soothing cosmetics. The moisturizing and allergy-relieving cosmetics on the market are usually added with beneficial components such as amino acid, hyaluronic acid, salvia miltiorrhiza extract and the like to solve the problems of dry and sensitive skin.

CN108653158A discloses a cosmetic with an auxiliary repairing effect on sensitive skin, which is prepared from the following raw materials in parts by weight: 10 parts of fresh mulberry leaves, 5 parts of fructus kochiae, 5 parts of nidus vespae, 1 part of snake slough, 1 part of fructus forsythiae, 3 parts of honeysuckle, 10 parts of centella, 5 parts of purified water, 10 parts of coconut oil, 3 parts of natural emulsifier and 0.1 part of xanthan gum. The product is simple to prepare, has no irritation to skin, and can only solve the problem of skin sensitivity but not solve the problem of skin dryness.

CN109453083A discloses a moisturizing and allergy-relieving composition and application thereof, wherein the composition comprises the following components: yeast extract, grapefruit fruit extract, crataegus cuneata fruit extract and chamomile extract, wherein the yeast extract is easy to be absorbed by skin and has the moisturizing effect, but the problem of dry skin cannot be fundamentally solved.

CN106852836A discloses a preparation method of a moisturizing night cream, which comprises allantoin, hyaluronic acid, chamomile extract, aloe extract, arnica extract, liquorice extract, forsythia extract, witch hazel extract and the like, has the effects of improving the tightness, anti-allergy, anti-inflammation and itching-relieving effects of washed dry skin, but cannot increase the water content of stratum corneum and strengthen the barrier function of the skin.

In conclusion, the existing moisturizing and allergy-relieving cosmetics mainly comprise mild components, have no stimulation to skin, can only relieve the problem of skin, and cannot fundamentally solve the problems of dry and sensitive skin caused by reduction of skin barrier function and inflammatory reaction of skin surface. Therefore, how to provide a moisture-retaining and allergy-soothing composition having the functions of improving skin barrier function, water-retaining capacity and anti-inflammatory effect has become one of the problems to be solved at present.

Disclosure of Invention

Aiming at the defects and actual requirements of the prior art, the invention provides a moisturizing and allergy-relieving composition and application thereof, wherein the composition has the effects of strengthening the barrier function of skin, increasing the water content of stratum corneum and repairing inflammatory reaction, and can fundamentally solve the problems of dry and sensitive skin.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a moisturizing and allergy-relieving composition, which comprises white pear juice, ganoderma lucidum extract, honeysuckle flower extract, forsythia suspense extract, amino acids and aloe leaf water.

According to the invention, the white pear juice is prepared by cold pressing and sterilizing white pear fruits, contains abundant components such as polysaccharide, polyphenol, arbutin, vitamin C, vitamin E and the like, and has the characteristics of long-acting moisture preservation, protection of skin tissues from being damaged by free radicals, reduction of melanin deposition, inhibition of pathogenic bacteria, anti-aging and anti-inflammation and the like;

the ganoderma lucidum extract is prepared by carrying out superfine grinding technology and low-temperature reduced pressure extraction treatment on ganoderma lucidum, the ganoderma lucidum extract is rich in various active ingredients such as polysaccharide, triterpenes, peptidoglycan, protein, alkaloid, trace elements and the like, polysaccharide molecules in the ganoderma lucidum extract can form hydrogen bonds with water molecules in skin to play a role in moisture preservation, in addition, the polysaccharide molecules can form a layer of compact film on the surface of the skin to reduce water loss and achieve the effect of water locking, can provide nutrient components for cells to ensure that the skin is moist and full, and the ganoderma lucidum extract can also play an anti-inflammatory role by inhibiting NO, prostaglandin E2, interleukin 1 beta secretion and other ways;

the honeysuckle flower extract is prepared by processing honeysuckle flowers through a low-temperature reduced pressure extraction technology, can inhibit the synthesis of inflammatory factors and slow down the release of the inflammatory factors, plays an anti-inflammatory role, is natural and non-irritant, can promote the growth of dominant bacteria such as bifidobacteria and lactobacillus, and maintains the skin micro-ecology;

the fructus forsythiae extract is prepared by processing fructus forsythiae through a low-temperature reduced pressure extraction technology, the main components comprise phenylethanoid glycosides, lignans, phenolic acids, triterpenes, diterpenes and the like, and the fructus forsythiae extract has the function of inhibiting NO secretion, so that the antibacterial and anti-inflammatory effects are achieved;

the amino acid is an important component in the natural moisturizing factor, can help keratinocytes absorb water and maintain a hydration function, can also promote a metabolic reaction of enzyme, and is helpful for stratum corneum differentiation and maturation, so that the skin is restored to be moist, and the barrier function of the skin is enhanced;

the aloe leaf water is prepared by peeling and juicing fresh leaves of aloe vera and carrying out filtering and sterilizing treatment, contains various components such as polysaccharide, anthraquinone, protein, organic acid, amino acid, trace elements and the like, can permeate into skin layer by layer, has the effects of enhancing immunity, resisting bacteria and inflammation, quickly replenishing water, resisting oxidation and the like, and can relieve and repair the skin.

According to the moisturizing and allergy-relieving composition, the white pear juice and the lucid ganoderma extract in the moisturizing and allergy-relieving composition exert the effects of water locking, moisturizing, bacteriostasis and anti-inflammation, the honeysuckle flower extract and the forsythia extract are mutually matched to enhance the anti-inflammation effect of the composition, the amino acid enhances the barrier function of the skin, the aloe leaf water is used as a solvent for the white pear juice, the lucid ganoderma extract, the honeysuckle flower extract, the forsythia extract and the amino acid, the moisturizing and anti-oxidation effects of the composition are further enhanced, the components are synergistic, the moisturizing and allergy-relieving effects are achieved from the aspects of enhancing the barrier function of the skin, increasing the water content of the horny layer, repairing inflammatory reaction and the like, and the targeted adjusting effect is achieved on dry and sensitive skin.

Preferably, the amino acid comprises any one of proline, alanine or serine or a combination of at least two thereof.

Preferably, the mass percentage of the white pear juice in the composition is 1% to 15%, such as 2%, 3%, 4%, 5%, 8%, 11%, 12%, 13% or 14%, preferably 5% to 15%, and more preferably 5% to 10%.

Preferably, the mass percentage of the ganoderma lucidum extract in the composition is 1% to 15%, such as 2%, 3%, 4%, 5%, 8%, 11%, 12%, 13% or 14%, preferably 5% to 15%, and more preferably 5% to 10%.

Preferably, the honeysuckle flower extract is 1% to 20% in the composition, such as 2%, 3%, 4%, 5%, 10%, 15%, 16%, 17%, 18% or 19%, preferably 5% to 20%, and more preferably 5% to 15% by mass.

Preferably, the mass percentage of the forsythia suspense extract in the composition is 1% to 20%, such as 2%, 3%, 4%, 5%, 10%, 15%, 16%, 17%, 18% or 19%, preferably 5% to 20%, and more preferably 5% to 15%.

Preferably, the amino acid is present in the composition in a mass percentage of 1% to 15%, such as 2%, 3%, 4%, 5%, 8%, 11%, 12%, 13% or 14%, preferably 1% to 10%, more preferably 1% to 5%.

Preferably, the composition comprises, in mass percent: 1-15% of white pear juice, 1-15% of lucid ganoderma extract, 1-20% of honeysuckle flower extract, 1-20% of forsythia extract, 1-15% of amino acid and the balance of aloe leaf water.

Preferably, the composition comprises, in mass percent: 5-15% of white pear juice, 5-15% of lucid ganoderma extract, 5-20% of honeysuckle flower extract, 5-20% of forsythia extract, 1-10% of amino acid and the balance of aloe leaf water.

Preferably, the composition comprises, in mass percent: 5-10% of white pear juice, 5-10% of lucid ganoderma extract, 5-15% of honeysuckle flower extract, 5-15% of forsythia extract, 1-5% of amino acid and the balance of aloe leaf water.

In a second aspect, the present invention provides a process for preparing a composition according to the first aspect, the process comprising the steps of:

adding white pear juice, Ganoderma extract, flos Lonicerae extract, fructus forsythiae extract and amino acids into folium Aloe water at a certain proportion, mixing well, and making into the composition.

Preferably, the mixing time is 5-30 min, such as 6min, 7min, 8min, 9min, 18min, 23min, 26min, 27min, 28min or 29min, but is not limited to the recited values, and other values not recited in the range of values are also applicable.

Preferably, the temperature of the mixing is 25 to 45 ℃, such as 26 ℃, 27 ℃, 28 ℃, 29 ℃, 35 ℃, 40 ℃, 41 ℃, 42 ℃, 43 ℃ or 44 ℃, but is not limited to the recited values, and other values not recited in the range of the values are also applicable.

In a third aspect, the present invention provides the use of a composition according to the first aspect for the preparation of a cosmetic product.

In a fourth aspect, the present invention provides a cosmetic comprising the composition of the first aspect.

Preferably, the composition is added to the cosmetic in an amount of 0.1% to 10%, such as 0.2%, 0.3%, 0.4%, 0.5%, 1%, 4%, 7%, 8% or 9%, more preferably 1% to 5% by weight of the total cosmetic mass.

Preferably, the cosmetic further comprises any one or a combination of at least two of a silicon-containing emulsifier, oil, thickener or humectant.

Preferably, the cosmetic further comprises a preservative and/or a fragrance.

Preferably, the formulation of the cosmetic comprises any one or a combination of at least two of a water agent, an emulsion, a cream, a essence, a gel, a mask, a spray or a base makeup.

Compared with the prior art, the invention has the following beneficial effects:

(1) the components of the moisturizing and allergy-relieving composition disclosed by the invention are matched for use, can play a synergistic role, can achieve the moisturizing and allergy-relieving effects in multiple aspects of strengthening skin barriers, improving the water content of stratum corneum, repairing inflammatory reaction and the like, and plays a role in pertinently regulating dry and sensitive skin in the whole skin renewal process.

(2) The moisturizing and allergy-relieving composition disclosed by the invention is simple in preparation process, adopts natural plants as main raw materials, and is beneficial to sustainable development.

(3) The cosmetics prepared from the moisturizing and allergy-relieving composition has remarkable moisturizing and allergy-relieving effects, and is specifically characterized in that the transdermal water dispersion amount of a tested volunteer is below 6%, the moisture content of the skin stratum corneum is above 22%, and the satisfaction degree of the tested volunteer on the products is high. Therefore, the moisturizing and allergy-relieving composition has an important application prospect in the field of moisturizing and allergy-relieving cosmetics.

Detailed Description

To further illustrate the technical means and effects of the present invention, the present invention is further described with reference to the following examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention.

The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or apparatus used are conventional products commercially available from normal sources, not indicated by the manufacturer.

The raw material sources are as follows: the white pear juice, the ganoderma lucidum extract, the honeysuckle flower extract and the forsythia suspense extract are provided by Guangzhou Huaya cosmetics science and technology limited, and the amino acid and the aloe leaf water are commercially available.

Example 1

The embodiment provides a moisturizing and allergy-relieving composition, which is composed of the following components in percentage by mass: 10% of white pear juice, 10% of lucid ganoderma extract, 15% of honeysuckle flower extract, 15% of fructus forsythiae extract, 5% of proline and 45% of aloe leaf water.

The preparation method comprises the following steps: adding white pear juice, Ganoderma extract, flos Lonicerae extract, fructus forsythiae extract and proline into folium Aloe water at a certain proportion, stirring at 45 deg.C for 30min, and mixing to obtain the composition with effects of keeping moisture and relieving allergy.

Example 2

The embodiment provides a moisturizing and allergy-relieving composition, which is composed of the following components in percentage by mass: 8% of white pear juice, 8% of lucid ganoderma extract, 15% of honeysuckle flower extract, 15% of fructus forsythiae extract, 5% of alanine and 49% of aloe leaf water.

The preparation method comprises the following steps: adding white pear juice, Ganoderma extract, flos Lonicerae extract, fructus forsythiae extract and alanine into folium Aloe water at a certain proportion, stirring at 35 deg.C for 20min, and mixing to obtain the composition with effects of keeping moisture and relieving allergy.

Example 3

The embodiment provides a moisturizing and allergy-relieving composition, which is composed of the following components in percentage by mass: 5% of white pear juice, 5% of lucid ganoderma extract, 15% of honeysuckle flower extract, 15% of forsythia extract, 5% of serine and 55% of aloe leaf water.

The preparation method comprises the following steps: adding white pear juice, Ganoderma extract, flos Lonicerae extract, fructus forsythiae extract and serine into folium Aloe water at a certain proportion, stirring at 35 deg.C for 10min, and mixing to obtain the composition with effects of keeping moisture and relieving allergy.

Example 4

The embodiment provides a moisturizing and allergy-relieving composition, which is composed of the following components in percentage by mass: 8% of white pear juice, 8% of lucid ganoderma extract, 10% of honeysuckle flower extract, 10% of fructus forsythiae extract, 5% of proline and 59% of aloe leaf water.

The preparation method comprises the following steps: adding white pear juice, Ganoderma extract, flos Lonicerae extract, fructus forsythiae extract and proline into folium Aloe water at a certain proportion, stirring at 25 deg.C for 30min, and mixing to obtain the composition with effects of keeping moisture and relieving allergy.

Example 5

The embodiment provides a moisturizing and allergy-relieving composition, which is composed of the following components in percentage by mass: 8% of white pear juice, 8% of lucid ganoderma extract, 5% of honeysuckle flower extract, 5% of fructus forsythiae extract, 5% of proline and 69% of aloe leaf water.

The preparation method comprises the following steps: adding white pear juice, Ganoderma extract, flos Lonicerae extract, fructus forsythiae extract and proline into folium Aloe water at a certain proportion, stirring at 25 deg.C for 20min, and mixing to obtain the composition with effects of keeping moisture and relieving allergy.

Example 6

The embodiment provides a moisturizing and allergy-relieving composition, which is composed of the following components in percentage by mass: 8% of white pear juice, 8% of lucid ganoderma extract, 10% of honeysuckle flower extract, 10% of fructus forsythiae extract, 3% of proline and 61% of aloe leaf water.

The preparation method comprises the following steps: adding white pear juice, Ganoderma extract, flos Lonicerae extract, fructus forsythiae extract and proline into folium Aloe water at a certain proportion, stirring at 25 deg.C for 10min, and mixing to obtain the composition with effects of keeping moisture and relieving allergy.

Example 7

The embodiment provides a moisturizing and allergy-relieving composition, which is composed of the following components in percentage by mass: 8% of white pear juice, 8% of lucid ganoderma extract, 10% of honeysuckle flower extract, 10% of fructus forsythiae extract, 1% of proline and 63% of aloe leaf water.

Comparative example 1

The same as example 1, except that the moisture-keeping and allergy-relieving composition comprises 100% of aloe leaf water by mass percent.

Comparative example 2

The same as example 1, except that the moisture-keeping and allergy-relieving composition comprises 40% of white pear juice and 60% of deionized water by mass percent.

Comparative example 3

The difference is that the moisture-keeping and allergy-relieving composition is composed of 40% of ganoderma lucidum extract and 60% of deionized water by mass percent as same as example 1.

Comparative example 4

The difference is that the moisture-keeping and allergy-relieving composition is composed of 40% of honeysuckle flower extract and 60% of deionized water in percentage by mass as same as example 1.

Comparative example 5

The difference is that the moisturizing and allergy-relieving composition is composed of 40% of forsythia suspense extract and 60% of deionized water by mass percent as in example 1.

Comparative example 6

The same as example 1, except that the moisture-keeping and allergy-relieving composition comprises 40% of proline and 60% of deionized water by mass percent.

Comparative example 7

The same as example 1, except that the moisture-keeping and allergy-relieving composition comprises 40% of aloe leaf water and 60% of deionized water by mass percent.

Comparative example 8

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: 8% of white pear juice, 8% of lucid ganoderma extract, 10% of honeysuckle flower extract, 10% of forsythia suspense extract and 64% of deionized water, and the preparation method is the same as that of example 1.

Comparative example 9

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: 8% of white pear juice, 8% of lucid ganoderma extract, 10% of honeysuckle flower extract and 74% of aloe leaf water, and the preparation method is the same as that of example 1.

Comparative example 10

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: 8% of white pear juice, 8% of ganoderma lucidum extract, 3% of serine and 81% of aloe leaf water, and the preparation method is the same as that of example 1.

Comparative example 11

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: 8% of white pear juice, 10% of forsythia extract, 3% of proline and 79% of aloe leaf water, and the preparation method is the same as that of example 1.

Comparative example 12

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: honeysuckle flower extract 10%, forsythia suspensa extract 10%, proline 3% and aloe leaf water 77%, and the preparation method is the same as example 1.

Comparative example 13

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: the preparation method is the same as that of example 1, wherein the preparation method comprises 8% of ganoderma lucidum extract, 10% of honeysuckle flower extract, 10% of forsythia extract, 3% of proline and 69% of deionized water.

Comparative example 14

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: 8% of white pear juice, 8% of lucid ganoderma extract, 10% of honeysuckle flower extract, 10% of forsythia extract, 3% of proline and 61% of deionized water, and the preparation method is the same as that of example 1.

Comparative example 15

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: 8% of white pear juice, 8% of ganoderma lucidum extract, 10% of honeysuckle flower extract, 3% of proline and 71% of aloe leaf water, and the preparation method is the same as that of example 1.

Comparative example 16

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: 8% of white pear juice, 8% of ganoderma lucidum extract, 10% of forsythia extract, 3% of proline and 71% of aloe leaf water, and the preparation method is the same as that of example 1.

Comparative example 17

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: 8% of white pear juice, 10% of honeysuckle flower extract, 10% of forsythia extract, 3% of proline and 69% of aloe leaf water, and the preparation method is the same as that of example 1.

Comparative example 18

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: the preparation method is the same as that of example 1, wherein the ganoderma lucidum extract is 8%, the honeysuckle flower extract is 10%, the forsythia suspense extract is 10%, the proline is 3% and the aloe leaf water is 69%.

Comparative example 19

The difference is only that the moisturizing and allergy-relieving composition is composed of the following components in percentage by mass: 8% of white pear juice, 8% of ganoderma lucidum extract, 10% of honeysuckle flower extract, 10% of forsythia suspense extract and 64% of aloe leaf water, and the preparation method is the same as that of example 1.

Test example 1: haemolysis test of erythrocytes

Red blood cell haemolysis (RBC) is one of the alternatives to rabbit eye irritation, and the basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of hemoglobin dissolved out of cells and the degree of denaturation. The RBC test can be used to evaluate the eye irritation of cosmetics.

The moisturizing and soothing composition prepared in examples 1 to 7 and the composition prepared in comparative examples 1 to 19 were subjected to an erythrocyte hemolysis experiment. RBC experimental test method and judgment standard of European substitution method verification center are adopted in experiment, and specific judgment standard is shown in Table 1, wherein HD₅₀The concentration of the sample at which 50% of erythrocytes are hemolyzed, DI is the protein denaturation index, and L/D is HD₅₀Ratio to DI.

TABLE 1

L/D	Grading
		L/D﹥100	Has no irritation
10﹤L/D≦100	Micro-stimulation property
		1﹤L/D≦10	Mild irritation
0.1﹤L/D≦1	Moderate irritation
		L/D≦0.1	Severe irritation

The irritation results of each of the samples prepared in examples 1 to 7 and comparative examples 1 to 19 are shown in table 2.

TABLE 2

As can be seen from the irritation results of the samples in table 2, the irritation of the moisturizing and allergy-soothing composition prepared in the embodiments 1 to 7 of the present invention is classified as non-irritation, which indicates that the moisturizing and allergy-soothing composition of the present invention has the characteristics of being mild and non-irritating; the irritation of the moisturizing and allergy-relieving composition prepared from the compositions in comparative examples 1 to 19 is classified as no irritation, but the L/D value of the moisturizing and allergy-relieving composition is lower than that of the moisturizing and allergy-relieving composition prepared from examples 1 to 7, which shows that 6 active ingredients are matched with each other, have synergistic effect and can obviously reduce the toxic and side effects and irritation of the composition.

Test example 2: skin barrier enhancement experiments

Trans Epidermal Water Loss (TEWL), also known as transdermal Water Loss, represents the Loss of Water in the deep dermis by evaporation through the epidermis, an important parameter describing the barrier function of the skin, which is closely related to the Water content of the stratum corneum of the skin. Lower TEWL values indicate less water loss through the skin and better barrier function of the stratum corneum; conversely, the poorer the barrier function. The volunteers who applied the product were tested using a moisture transdermal loss tester (Tewameter TM 300).

156 volunteers of 20-45 years old were randomly selected as study subjects, wherein 78 of the volunteers were male and female, and the tested part had no skin problems and no history of systemic diseases in the last month. Volunteers were randomly divided into 26 groups, and before the test, volunteers were asked to wipe both arms with clear water, sit still for 30min in a constant temperature and humidity environment (temperature: 25. + -. 1 ℃ C., relative humidity: 40. + -. 5%) and keep relaxed. Marking a 4cm multiplied by 4cm test area at a position 5cm away from the palm base part on the inner side of the arm of the volunteer, simultaneously marking a plurality of areas (with the interval of 1cm) on the same arm, and randomly smearing the test product to be tested on the marked area. The product (the moisturizing and soothing composition prepared in examples 1-7 and comparative examples 1-19) was applied to the marked area of the volunteer continuously around the circumference in an amount of 2.0 + -0.1 μ L/cm²And the marked area is not smearable with drugs or cosmetics not relevant to the test during the test period, and the loss of moisture through the skin was tested and recorded before, at week 1, 2 and 4 of the smear (TEWL0, TEWL1, TEWL2, TEWL4), the test results are shown in table 3, and the data shown are the average of the experimental data obtained.

TABLE 3

As can be seen from table 3, as the test time increases, the lower the transdermal moisture dispersion amount of the volunteers, the lower the transdermal moisture dispersion amount of the active ingredients 6 according to the present invention, the transdermal moisture dispersion amount of the volunteers using the moisturizing and soothing sensitivity composition prepared in examples 1 to 7 was 8% or less, the transdermal moisture dispersion amount of the volunteers using the moisturizing and soothing sensitivity composition prepared in comparative examples 1 to 7 was 9.5% to 11%, and the transdermal moisture dispersion amount of the volunteers using the composition prepared in comparative examples 8 to 19 was 8% to 9.5% at week 4 of the test of the volunteers. From the above, the transdermal water dispersion loss of the volunteers using the compositions prepared in comparative examples 1 to 19 was higher than that of the volunteers using the compositions prepared in examples 1 to 7, which shows that the mutual cooperation of the 6 active ingredients according to the present invention can make the transdermal water dispersion loss of the users lower than that of the users using the compositions of single active ingredient or lacking any active ingredient, and has synergistic effect and good function of repairing skin barrier.

Test example 3: skin stratum corneum moisture test

The skin moisture test is based on the capacitance measurement principle, and measures the change of the moisture content of the stratum corneum of the skin by measuring the change of the dielectric constant of the surface of the skin, thereby evaluating the moisturizing effect of the product. The volunteers who applied the product were tested using a skin moisture content test probe (Corneometer CM 825).

156 volunteers with dry skin were selected as study subjects, the age of the volunteers was 20-45 years, 78 of the volunteers were male and female, the tested parts had no skin problems in the last month, and no history of systemic diseases. Volunteers were randomly divided into 26 groups, and before the test, volunteers were asked to wipe both arms with clear water, sit still for 30min in a constant temperature and humidity environment (temperature: 25. + -. 1 ℃ C., relative humidity: 40. + -. 5%) and keep relaxed. Marking a 4cm multiplied by 4cm test area at a position 5cm away from the palm base part on the inner side of the arm of the volunteer, simultaneously marking a plurality of areas (with the interval of 1cm) on the same arm, and randomly smearing the test product to be tested on the marked area. Continuous peripheral applicationThe amount of the moisturizing and soothing composition (prepared in examples 1-7 and comparative examples 1-19) applied to the marked area is 2.0 +/-0.1 muL/cm²And the test period marked area is not smearable with drugs or cosmetics unrelated to the test, the moisture content of the stratum corneum was tested and recorded before smearing, at week 1 smearing, at week 2 smearing and at week 4 smearing (SM0, SM1, SM2, SM4), the test results are shown in table 4, and the data shown are the average of the experimental data obtained.

TABLE 4

As can be seen from table 4, the longer the product is applied to the skin of the volunteer, the higher the moisture content of the stratum corneum of the skin is, that is, the 6 active ingredients of the present invention can provide moisture to the skin, and the moisturizing and allergy-relaxing compositions prepared in examples 1 to 7 have better moisturizing effects compared with comparative examples 1 to 7 and comparative examples 8 to 19, can significantly increase the moisture content of the stratum corneum of the skin and reduce the moisture loss of the skin, and are specifically represented as follows: the moisture content of the stratum corneum of 1 week of volunteers who applied the moisturizing and allergy-soothing compositions prepared in examples 1 to 7, comparative examples 1 to 7 and comparative examples 8 to 19 respectively was: tg is 11.5%, 9% -10.5% and 10.5% -12%, the moisture content of the skin cuticle at the 4 th week is respectively as follows: tg is 19%, 11% -13% and 15% -17.5%. The 6 active ingredients in the moisturizing and allergy-relieving composition are scientifically proportioned, so that the moisture content of the skin stratum corneum of a user is higher than that of the skin stratum corneum of the user who uses the composition only containing a single active ingredient or lacking any active ingredient, and the moisturizing effect is obvious.

Test example 4: experiment for inhibiting hyaluronidase activity

Hyaluronidase is a specific lyase for hyaluronic acid and is commonly used to assess in vitro anti-allergic activity. Hyaluronic acid plays a major inhibitory role in human development and regulation (e.g., cell adhesion, organ formation, wound healing, tumorigenesis, and angiogenesis), and inhibition of hyaluronidase activity prevents sodium hyaluronate from being decomposed and maintains normal physiological functions, so hyaluronidase inhibition rate is used as an antiallergic standard, and specific classification standards are shown in table 5.

TABLE 5

Inhibition rate	Grading
		≧70％	Strong antiallergic effect
≧50％	Moderate antiallergic effect
		[30％,50％]	Weak antiallergic effect
﹤30％	Has no antiallergic effect

0.1mL of 0.25mmol/L CaCl was removed₂The solution, 0.5mL of 500U/mL hyaluronidase solution and a proper amount of acetic acid buffer solution (pH is 5.6) are put into 20 conical flasks and are gently shaken and uniformly mixed, the conical flasks are placed in a constant temperature incubator at 37 ℃ for incubation for 20min, then 0.5mL of deionized water and 0.5mL of sample solution (prepared by the moisturizing and allergy-relieving compositions prepared in examples 1-7 and comparative examples 1-19, the concentration is 50mg/mL) are sequentially added, the conical flasks are placed at 37 ℃ for incubation for 20min, and then 0.5mL of 0.5mg/mL of potassium hyaluronidase solution and a proper amount of acetic acid slow release composition are addedThe washing liquid (pH is 5.6), is placed at 37 ℃ for 40min, is placed at room temperature for 10min, is added with 0.5mL of deionized water, 1 drop of 5mol/L NaOH solution and 1mL of acetylacetone solution, is placed in a boiling water bath for heating for 15min, is immediately cooled for 5min by ice water, is placed at room temperature for 10min, is added with 1mL of P-DAB color developing agent and a proper amount of absolute ethyl alcohol, is placed at room temperature for 30min for color development, is used for measuring the absorbance value of the sample at 530nm by using a spectrophotometer, calculates the inhibition rate of each group of samples on hyaluronidase, takes dipotassium glycyrrhizinate solution with the mass fraction of 5% as a positive control, and the calculation formula is shown as (1-1) and the calculation results are shown in Table 6.

Wherein: i is the hyaluronidase inhibition rate;

A₁is the absorbance value of the (hyaluronidase + sample + hyaluronidase potassium) solution;

A₂is the absorbance value of the solution of (acetic acid buffer solution + sample + acetic acid buffer solution);

A₃the absorbance value of the (hyaluronidase + deionized water + hyaluronidase potassium) solution is shown;

A₄the absorbance value of the solution is (acetic acid buffer solution + deionized water).

TABLE 6

As can be seen from the results of the hyaluronidase inhibition rates in table 5, the inhibition rates of the moisturizing and allergy-relieving compositions prepared in examples 1 to 7 on hyaluronidase all reach over 90%, and are higher than those of the compositions in comparative examples 1 to 19 and dipotassium glycyrrhizinate, which indicates that the 6 active ingredients are matched with each other, the hyaluronidase inhibition rates of the compositions are higher than those of the compositions containing only a single active ingredient or lacking any active ingredient, and the components are synergistic, so that the anti-allergic capability of the skin is remarkably improved.

Application example 1

A makeup-removing spray is prepared by using the moisturizing and allergy-relieving composition prepared in example 1, and the mass percentage of each component is shown in table 7.

TABLE 7

The preparation method of application example 1 is as follows:

(1) adding the component A into an emulsifying pot, and stirring and dissolving the component A uniformly at 80 ℃;

(2) dispersing and dissolving the component B uniformly in advance, adding the component B into an emulsifying pot at the temperature of 80 ℃, stirring and dispersing uniformly;

(3) cooling to 60 ℃, adding the component C, and stirring to dissolve uniformly;

(4) cooling to 45 ℃, adding the components D and E into an emulsifying pot, and stirring and dissolving uniformly;

(5) cooling to room temperature, and vacuum defoaming to obtain the moisturizing, allergy-relieving and makeup-fixing spray.

Application example 2

The difference from application example 1 is that the moisturizing and soothing composition prepared in example 1 in the E component is replaced by the composition prepared in example 2, and the content of other components and the preparation method are not changed.

Application example 3

The difference from application example 1 is that the moisturizing and soothing composition prepared in example 1 in the E component is replaced by the composition prepared in example 3, and the content of other components and the preparation method are not changed.

Application example 4

The difference from application example 1 is that the moisturizing and soothing composition prepared in example 1 in the E component is replaced by the composition prepared in example 4, and the content of other components and the preparation method are not changed.

Application example 5

The difference is that the weight percentage of the moisturizing and allergy-relieving combination in the component E is 0.1 percent, and the contents of other components and the preparation method are not changed with the same application example 1.

Application example 6

The difference is that the weight percentage of the moisturizing and allergy-relieving combination in the component E is 1 percent, and the contents of other components and the preparation method are not changed with the same application example 1.

Application example 7

The difference is that the weight percentage of the moisturizing and allergy-relieving combination in the component E is 5 percent, and the contents of other components and the preparation method are not changed with the same application example 1.

Application example 8

The difference is that the weight percentage of the moisturizing and allergy-relieving combination in the component E is 10 percent, and the contents of other components and the preparation method are not changed with the same application example 1.

Application comparative example 1

The difference from application example 1 is that the moisture-keeping and allergy-relieving composition prepared in example 1 in the component E is replaced by the composition prepared in comparative example 1, and the content of other components and the preparation method are not changed.

Comparative application example 2

The difference from application example 1 is that the moisture-keeping and allergy-relieving composition prepared in example 1 in the component E is replaced by the composition prepared in comparative example 2, and the content of other components and the preparation method are not changed.

Comparative application example 3

The difference from application example 1 is that the moisture-keeping and allergy-relieving composition prepared in example 1 in the component E is replaced by the composition prepared in comparative example 3, and the content of other components and the preparation method are not changed.

Application comparative example 4

The difference from application example 1 is that the moisture and allergy relieving composition prepared in example 1 in the component E is replaced by the composition prepared in comparative example 4, and the content of other components and the preparation method are not changed.

Comparative application example 5

The difference from application example 1 is that the moisture-keeping and allergy-relieving composition prepared in example 1 in the component E is replaced by the composition prepared in comparative example 5, and the content of other components and the preparation method are not changed.

Comparative application example 6

The difference from application example 1 is that the moisture-keeping and allergy-relieving composition prepared in example 1 in the component E is replaced by the composition prepared in comparative example 6, and the content of other components and the preparation method are not changed.

Application comparative example 7

The difference from application example 1 is that the moisture-retaining and soothing composition prepared in example 1 in the E component is replaced by the composition prepared in comparative example 7, and the contents of other components and the preparation method are not changed.

Comparative application example 8

The difference from application example 1 is that the moisture-retaining and soothing composition prepared in example 1 in the E component is replaced by the composition prepared in comparative example 8, and the contents of other components and the preparation method are not changed.

Comparative application 9

The difference from application example 1 is that the moisture-retaining and soothing composition prepared in example 1 in the E component is replaced by the composition prepared in comparative example 9, and the contents of other components and the preparation method are not changed.

Comparative application example 10

The difference from application example 1 is that the moisture-retaining and soothing composition prepared in example 1 in the E component is replaced by the composition prepared in comparative example 10, and the contents of other components and the preparation method are not changed.

Comparative application example 11

The difference from application example 1 is that the moisture-retaining and soothing composition prepared in example 1 in the E component is replaced by the composition prepared in comparative example 11, and the contents of other components and the preparation method are not changed.

Comparative application example 12

The difference from application example 1 is that the moisture and allergy relieving composition prepared in example 1 in the component E is replaced by the composition prepared in comparative example 12, and the content of other components and the preparation method are not changed.

Comparative application example 13

The difference from application example 1 is that the moisture and allergy relieving composition prepared in example 1 in the component E is replaced by the composition prepared in comparative example 13, and the content of other components and the preparation method are not changed.

Test example 5: skin barrier enhancement experiments

210 volunteers of 20-45 years old were randomly selected as study subjects, wherein 105 male and female patients had no skin problems at the tested part in the last month and no history of systemic diseases. Volunteers were randomly divided into 21 groups, and before the test, the volunteers were asked to wipe both arms with clear water, sit still for 30min in a constant temperature and humidity environment (temperature: 25. + -. 1 ℃ C., relative humidity: 40. + -. 5%) and keep relaxed. Marking a 4cm multiplied by 4cm test area at a position 5cm away from the palm base part on the inner side of the arm of a volunteer, simultaneously marking a plurality of areas (with intervals of 1cm) on the same arm, randomly coating the test products to be tested on the mark area, and continuously coating the products (the moisturizing and allergy-relieving fixed make-up spray prepared by application examples 1-8 and application comparative examples 1-13) on the mark area of the volunteer around the same arm, wherein the using amount is 2.0 +/-0.1 mu L/cm²And no drug or cosmetic not related to the experiment was applied during the test period, transdermal water loss (TEWL0, TEWL1, TEWL2, TEWL4) was tested and recorded using a moisture transdermal water loss tester (Tewameter TM300) before application, at week 1 of application, at week 2 of application, and at week 4 of application, the test results are shown in table 8, and the data shown are the average of the experimental data obtained.

TABLE 8

As can be seen from table 8, the transdermal water dispersion loss of the volunteers at week 4 was 9.74% and 7.46% respectively, which are higher than that of application example 1 in application examples 5-6; the transdermal water dispersion amount of the application examples 7-8 is similar to that of the application example 1, namely the effect of the moisturizing and allergy-relieving composition in the makeup-fixing spray is similar to that of the makeup-fixing spray when the mass percentage of the moisturizing and allergy-relieving composition is 2%, 5% and 10%, and the effect is superior to that of the makeup-fixing spray when the mass percentage of the moisturizing and allergy-relieving composition is 0.1% and 1%, and from the aspect of saving the production cost, the moisturizing and allergy-relieving composition with the mass percentage of 2% is preferably applied to the makeup-fixing spray.

The transdermal water dispersion loss of the volunteers shows a descending trend along with the increase of the test time, which shows that 6 active ingredients of the invention can enhance the skin barrier function, while at 4 weeks of the test of the volunteers, the transdermal water dispersion loss of the volunteers using the fixed-dressing sprays prepared in application examples 1-8 of the invention is less than 6%, the transdermal water dispersion loss of the volunteers using the fixed-dressing sprays prepared in application examples 1-7 is between 8% and 9%, the transdermal water dispersion loss of the volunteers using the fixed-dressing sprays prepared in application examples 8-13 is between 7.5% and 8%, and is higher than that of the volunteers using the fixed-dressing sprays prepared in examples 1-8, namely, the transdermal water dispersion loss of the volunteers containing a single active ingredient composition or the fixed-dressing sprays lacking a certain active ingredient composition is higher than that of the test volunteers containing 6 active ingredients, the makeup-removing spray prepared by the moisturizing and allergy-relieving composition containing 6 active ingredients has stronger skin repairing barrier function than the makeup-removing spray prepared by the composition containing only a single active ingredient or lacking any active ingredient.

Test example 6: determination of water content of horny layer

210 dry skin volunteers are selected as study objects, the ages of the volunteers are 20-45 years, wherein the skin problem does not exist in the tested part in nearly one month in half of men and women, and the history of the systemic diseases does not exist. Volunteers were randomly divided into 21 groups, and before the test, the volunteers were asked to wipe both arms with clear water, sit still for 30min in a constant temperature and humidity environment (temperature: 25. + -. 1 ℃ C., relative humidity: 40. + -. 5%) and keep relaxed. Marking a 4cm multiplied by 4cm test area at a position 5cm away from the palm base part on the inner side of the arm of the volunteer, simultaneously marking a plurality of areas (with the interval of 1cm) on the same arm, and testing a product to be testedThe cosmetic compositions were randomly applied to the marked area, and the product (moisturizing and soothing make-up fixing spray prepared in application examples 1-8 and comparative examples 1-13) was continuously applied to the marked area of the volunteers at the periphery in an amount of 2.0 + -0.1 muL/cm²And no drug or cosmetic not related to the experiment was applied during the test period, stratum corneum moisture content (SM0, SM1, SM2, SM4) was measured and recorded before application, at week 1 of application, at week 2 of application, and at week 4 of application using a skin moisture content test probe (Corneometer CM825), and the test results are shown in table 9, which is an average of the experimental data obtained.

TABLE 9

As can be seen from table 9, the transdermal water dispersion loss of the volunteers at week 4 was 10.73% and 18.62% for the application examples 5-6, respectively, which are lower than that of the application example 1; the transdermal water dispersion amount of the application examples 7-8 is similar to that of the application example 1, namely the moisturizing effect of the moisturizing and allergy-relieving composition in the makeup-free spray is similar to that of the makeup-free spray when the mass percentages of the moisturizing and allergy-relieving composition are 2%, 5% and 10%, and is better than that of the makeup-free spray when the mass percentages of the moisturizing and allergy-relieving composition are 0.1% and 1%, and from the perspective of saving the production cost, the moisturizing and allergy-relieving composition with the mass percentage of 2% is preferably applied to the makeup-free spray.

The moisture content of the skin stratum corneum of the volunteers increases along with the increase of the application time, namely 6 active ingredients in the moisturizing and allergy-relieving composition can provide moisture for the skin, compared with the makeup-fixing sprays prepared by the application comparative examples 1-7 and the application comparative examples 8-13, the makeup-fixing sprays prepared by the application examples 1-8 can obviously improve the moisture content of the skin stratum corneum of the volunteers and reduce the loss of the skin moisture, and the moisture content of the skin stratum corneum of the volunteers in 1 week after the application examples 1-8, the application comparative examples 1-7 and the makeup-fixing sprays prepared by the application comparative examples 8-13 are respectively: tss is 13.5%, 9-11% and 12-14.5%, and the moisture content of the skin cuticle at the 4 th week is respectively as follows: tg is 22%, 14% -16.5% and 20%. From the above, the makeup-fixing spray prepared by applying the moisturizing and allergy-relieving composition containing 6 active ingredients has an obvious moisturizing effect.

Test example 7: antiallergic test

340 volunteers with facial skin allergy are selected as study objects, wherein 170 volunteers are respectively selected from male and female, the volunteers are randomly divided into 21 groups, 1 sample is smeared on each group of volunteers, the left face and the right face of the volunteer are taken as test areas, after the face is cleaned in the morning and evening every day, the corresponding test samples are respectively used on the left face and the right face, the test period is 4 weeks, the test values indicate the number of the volunteers to be tested who approve the corresponding products, and the specific test results are shown in table 10.

Watch 10

As can be seen from table 10, in the volunteers who used the makeup mist prepared in application examples 5 to 6, the number of the tested volunteers who had no allergic symptoms and had no redness, swelling, and itching was less than that of the volunteers who used the makeup mist prepared in application examples 1 to 4; the recognition degree of the fixed-makeup spray prepared in application examples 7-8 on the volunteers of the fixed-makeup spray is close to the number of the volunteers of the fixed-makeup spray prepared in application example 1, namely the anti-allergic effect of the moisturizing and allergy-relieving composition in the fixed-makeup spray is similar to that of the fixed-makeup spray prepared in application example 1 when the mass percent of the moisturizing and allergy-relieving composition is 2%, 5% and 10%, and the moisturizing and allergy-relieving composition is superior to the fixed-makeup spray prepared by the moisturizing and allergy-relieving composition with the mass percent of 0.1% and 1%, and the moisturizing and allergy-relieving composition with the mass percent of 2% is preferably applied to the fixed-makeup spray from the perspective of saving.

In the volunteers who used the makeup fixing spray prepared in the application comparative examples 1 to 7, the allergic symptoms of most of the tested volunteers were not relieved, and the allergic symptoms of no volunteer completely disappeared; in the volunteers who used the makeup fixing spray prepared in the application comparative examples 8-13, the allergic symptoms of a few tested volunteers completely disappeared, but the number of people who had slightly improved symptoms and had not completely relieved the symptoms was more; in volunteers who used the makeup mist prepared in application examples 1 to 8, symptoms of all subjects improved to different degrees, and it is understood that pear juice, ganoderma lucidum extract, honeysuckle flower extract, forsythia suspensa extract, amino acids and aloe leaf water supplement each other, synergize, and maximize the allergy-relieving effect.

Test example 8: consumer sensory testing

In order to evaluate the actual use effect of the moisturizing and allergy-relieving composition, the makeup remover spray prepared in application example 1 of the invention is compared with the makeup remover sprays prepared in comparative examples 1-13 and a makeup remover spray with a commercially available moisturizing and allergy-relieving effect. 260 oily skin volunteers are selected as study objects, the volunteers are equally divided into 13 groups for sensory evaluation test, the test items are the mildness, the moisturizing effect, the allergy relieving effect, the make-up fixing effect and the overall evaluation of the product, the test period is 4 weeks, the test values indicate the number of volunteer subjects approving the corresponding product, and the specific test results are shown in table 11.

TABLE 11

By performing blind tests on the makeup remover spray prepared in application example 1, the makeup remover sprays prepared in application comparative examples 1 to 13, and the makeup remover spray with the moisturizing and allergy-relieving efficacy of the commercially available main product, it can be seen from the test results in table 11 that the number of approved people of the makeup remover spray prepared in application example 1 is higher than that of the makeup remover sprays prepared in application comparative examples 1 to 13 and the commercially available products, which indicates that the acceptance degree of the makeup remover spray prepared in application example 1 is the highest among the tested volunteers.

In conclusion, the active components of the moisturizing and allergy-relieving composition disclosed by the invention are mutually matched and synergically synergistic, so that a moisturizing and allergy-relieving effect which is more obvious than that of a single component or other combined forms is achieved. In addition, the actual use of consumers proves that the cosmetics prepared by the composition also have excellent moisturizing and allergy-relieving effects, and the consumer acceptance degree is higher compared with the similar products on the market.

The applicant states that the present invention is illustrated in detail by the above examples, but the present invention is not limited to the above detailed methods, i.e. it is not meant that the present invention must rely on the above detailed methods for its implementation. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims

1. A composition for moisturizing and soothing allergy is characterized by comprising white pear juice, ganoderma lucidum extract, honeysuckle flower extract, forsythia extract, amino acid and aloe leaf water.

2. The composition of claim 1, wherein the amino acid comprises any one of proline, alanine or serine or a combination of at least two thereof.

3. The composition according to claim 1 or 2, wherein the mass percentage of the white pear juice in the composition is 1% to 15%, preferably 5% to 15%, and more preferably 5% to 10%;

preferably, the mass percentage of the ganoderma lucidum extract in the composition is 1-15%, preferably 5-15%, and more preferably 5-10%;

preferably, the honeysuckle flower extract accounts for 1-20% of the composition by mass, preferably 5-20% of the composition by mass, and more preferably 5-15% of the composition by mass;

preferably, the mass percentage of the forsythia suspense extract in the composition is 1-20%, preferably 5-20%, and more preferably 5-15%;

preferably, the mass percentage of the amino acid in the composition is 1% to 15%, preferably 1% to 10%, and more preferably 1% to 5%.

4. The composition according to any one of claims 1 to 3, wherein the composition comprises, in mass percent: 1-15% of white pear juice, 1-15% of lucid ganoderma extract, 1-20% of honeysuckle flower extract, 1-20% of fructus forsythiae extract, 1-15% of amino acid and the balance of aloe leaf water;

preferably, the composition comprises, in mass percent: 5-15% of white pear juice, 5-15% of lucid ganoderma extract, 5-20% of honeysuckle flower extract, 5-20% of forsythia extract, 1-10% of amino acid and the balance of aloe leaf water;

5. A method of preparing the composition of any one of claims 1 to 4, comprising:

6. The method according to claim 5, wherein the mixing time is 5-30 min;

preferably, the mixing temperature is 25-45 ℃.

7. Use of a composition according to any one of claims 1 to 4 for the preparation of a cosmetic product.

8. A cosmetic characterized in that it comprises a composition according to any one of claims 1 to 4;

preferably, the composition is added to the cosmetic in an amount of 0.1% to 10%, preferably 1% to 5%, by weight of the total cosmetic mass.

9. The cosmetic according to claim 8, further comprising any one or a combination of at least two of a silicon-containing emulsifier, oil, thickener, or humectant;

preferably, the cosmetic further comprises a preservative and/or a fragrance.

10. The cosmetic according to claim 8 or 9, wherein the formulation of the cosmetic comprises any one or a combination of at least two of a water agent, an emulsion, a cream, a essence, a gel, a mask, a spray or a base makeup.