CN113637644A - 一种沙门氏菌噬菌体vB_SalP_TR2及其应用 - Google Patents
一种沙门氏菌噬菌体vB_SalP_TR2及其应用 Download PDFInfo
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Abstract
本发明提供了一种沙门氏菌噬菌体，所述噬菌体为沙门氏菌噬菌体vB_SalP_TR2，其保藏编号为：GDMCC No:61403‑B1，所述噬菌体属于短尾噬菌体，呈多面体的头部和短尾结构，头部长61.5～62.5nm，宽59～61nm，尾部长34～36nm。本发明中的噬菌体在pH2～11范围和温度4～80℃条件下具有良好的稳定性，不携带任何毒力或抗生素耐药基因，可以安全地运用于食品沙门氏菌的防控。
Description
技术领域
本发明属于微生物技术领域，尤其涉及一种沙门氏菌噬菌体vB_SalP_TR2及 其应用。
背景技术
沙门氏菌(Salmonella)属于肠杆菌科，革兰氏阴性肠道杆菌，是一种人畜 共患的致病菌，能够在人类、动物及环境间传播。沙门氏菌感染人类，主要是 食用了被污染的食品(特别是动物性食品，如鸡肉、猪肉和牛肉等)引起的。 近几十年来，由沙门氏菌引发的食物中毒占世界食物中毒病例的第一或第二位。 人体在感染沙门氏菌之后，一般会出现肠胃炎、腹泻、发热以及败血性等临床 症状，且免疫力下的人群(如老人、孕妇、婴幼儿等)，若不及时救治，可能因 衰竭而死亡。加强对食源性沙门氏菌的防控在食品安全领域具体极其重要的意 义。
目前，在食品加工过程中依旧使用干热、蒸汽、紫外、微波等物理灭菌， 但往往会带来营养价值的流失和产品感官特性的恶化。另一方面，在动物源产 品上过度使用抗生素，导致细菌耐药性上升及传播，同时还产生药物残留和环 境污染等一系列问题，因此针对沙门氏菌防控的新策略和产品亟待研究。噬菌 体是一种能够特异性吸附感染细菌的病毒，相比抗生素而言，具有特异性强、 来源广泛、增殖力强、不易产生抗性等特点，有望成为新的抗菌剂。市场上已 经有一些商业化的噬菌体产品，如荷兰EBI食品安全公司(EBI FoodSafety)将 噬菌体产品ListexTM P100用于肉类和奶酪产品中单增李斯特氏菌的防控。美国 食品药品监督管理局(U.S.Food and Drug Administration，FDA)先后批准了EcoShieldTM和Salmonelex等产品，可以添加到食品中以防控大肠杆菌O157:H7 和沙门氏菌等污染。
毫无疑问，噬菌体作为抗菌剂的前景是非常可观的，但依然有许多因素限 制其发展，主要以下几方面：
首先，噬菌体存在严格的宿主特异性，造成其宿主谱狭窄，单独使用显然 缺乏力度且使用范围受到限制。目前大部分的沙门氏菌噬菌体的裂解对象是 SalmonellaEnteritis和Salmonella Typhimurium，而针对其它血清型的噬菌体比较 少。
其次，一些噬菌体属于温和噬菌体，其DNA主要是以整合到细菌染色体上 的方式，与细菌共同繁殖，只有在遇到逆境胁迫的情况下，才会进入裂解期， 所以裂解时间较长。且细菌的致病基因嵌入到噬菌体的基因组上，极易导致致 病基因扩散等问题。
最后，噬菌体同样存在突变或失活，噬菌体制剂难以保存等特点。因此， 在运输和销售过程中，必须保持噬菌体的效价在有效的范围内，且确保在投放 应用的环境中，噬菌体能够稳定存活，发挥其作用。
目前缺乏能够同时解决以上问题而且遗传信息和理化性质清晰的沙门氏菌 噬菌体。
发明内容
为解决上述技术问题，本发明提供了一种新型沙门氏菌的噬菌体 vB_SalP_TR2，该噬菌体具有一定范围的裂解宿主谱，可以裂解Albany， Corvallis，Newport，Kottbus和Istanbul血清型沙门氏菌；该噬菌体可以单独或 与其他物质复配使用，为食品消毒净化提供一种安全、无毒的噬菌体产品。本 发明采用以下技术方案实现本发明的目的：
一种沙门氏菌噬菌体，申请人将其命名为：vB_SalP_TR2，所述噬菌体保藏 编号为：GDMCC No:61403-B1，分类名为：Salmonella enterica bacteriophage； 保藏时间：2021年02月26日；保藏单位：广东省微生物菌种保藏中心；保藏 地址：中国广州市先烈中路100号59号楼五楼广东省微生物研究所。该噬菌体 具有呈正二十面体的头部和可伸缩的尾部；噬菌体在固体培养基上可以形成透 亮空斑，周围无晕环，边缘清晰规则，直径为1～2mm；基因组核酸的酶切图谱 显示该噬菌体核酸是双链DNA(dsDNA)；根据国际病毒分类委员会(ICTV)2005 年发表的《病毒分类国际病毒分类委员会第八次报告》，vB_SalP_TR2属于短尾 病毒科(Podoviridae)。该噬菌体在4～60℃中放置1h，活性稳定；处于pH4和 pH11时，其效价与初始效价无显著性差异。
进一步地，所述噬菌体属于短尾噬菌体，呈多面体的头部和短尾结构，头 部长61.5～62.5nm，宽59～61nm，尾部长34～36nm。申请人通过在透射电镜下 进行观察，发现该噬菌体具有二十面体头部(长62±0.5nm和宽60±1nm)和短 的尾巴(长35±1nm)，符合Caudoviricetes Podoviridae的典型特征。因此，该 沙门氏菌噬菌体vB_SalP_TR2属于有尾病毒目，短尾噬菌体科。
进一步地，所述噬菌体vB_SalP_TR2的基因组序列如SEQ IDNO：1所示。 申请人通过对噬菌体vB_SalP_TR2全基因组测序及全基因组特征分析发现该噬 菌体的基因组序列如SEQ IDNO：1所示。
进一步地，所述噬菌体vB_SalP_TR2不包含细菌耐药或/和毒力基因。由于 本发明的噬菌体不含有耐药或/和毒力基因，在食品应用上具有安全性。申请人 通过对噬菌体vB_SalP_TR2全基因组特征分析发现在噬菌体vB_SalP_TR2的基 因组中，并未发现编码于目前己知毒力或耐药性相关的基因，因此噬菌体 vB_SalP_TR2可以安全应用于食品领域噬菌体防控。
进一步地，所述噬菌体vB_SalP_TR2在pH值2～11、在4～80℃条件下具有 耐性。发明人通过大量试验研究发现，噬菌体vB_SalP_TR2在pH值2～11、在 4～80℃条件下仍保持一定的裂解作用。
本发明还提供了所述噬菌体vB_SalP_TR2在制备抑制或杀灭沙门氏菌菌株 制剂中的应用。
进一步地，所述制剂为喷雾剂或洗剂。由于所述噬菌体vB_SalP_TR2具有 较好的亲水相，容易配制成液体型的喷雾或洗剂，对食品中的沙门氏菌的污染 可进行有效的杀灭。申请人将所述噬菌体vB_SalP_TR2的浓缩液用于在牛奶和 鸡肉中对SalmonellaAlbany的杀灭，发现能实现对一定数量沙门氏菌的防控， 抑制Salmonella Albany的增殖。
进一步地，所述应用为在食品杀菌中的应用。
本发明还提供了一种含有本发明噬菌体vB_SalP_TR2的组合物，所述组合 物至少含有本发明的噬菌体vB_SalP_TR2。
与现有技术相比，本发明的有益效果为：
(1)本发明噬菌体vB_SalP_TR2具有较强的杀灭活性和一定范围的裂解 谱，可针对性地杀灭Salmonella Corvallis，Salmonella Newport，Salmonella Albany，Salmonella Istanbul和Salmonella Kottbus五种常见的血清型沙门氏菌， 可应用于制备防治沙门氏菌的药物制剂中实现广谱杀菌的效果。
(2)本发明噬菌体vB_SalP_TR2不含有毒力或耐药性相关的基因，因此， 毒副作用小，安全性高，由于其具有较好的亲水相，容易配制成液体型的喷雾 或洗剂，对食品中的沙门氏菌的污染可进行有效的杀灭。
(3)本发明噬菌体vB_SalP_TR2在4～60℃条件下和pH值4～11范围内， 可保持较高的活性，能够在酸碱性的极端环境保持杀菌活性，有效的控制沙门 氏菌的增殖，扩宽了沙门氏菌防治制剂的使用范围。
附图说明
图1噬菌体vB_SalP_TR2电镜图。
图2噬菌体vB_SalP_TR2基因热图。
图3噬菌体vB_SalP_TR2的MOI示意图。
图4噬菌体vB_SalP_TR2的温度稳定性示意图。
图5噬菌体vB_SalP_TR2的pH稳定性示意图。
图6噬菌体vB_SalP_TR2一步生长曲线图。
图7噬菌体vB_SalP_TR2体外防控效果图。
图8噬菌体vB_SalP_TR2在牛奶中的抑菌作用示意图。
图9噬菌体vB_SalP_TR2在鸡肉中的抑菌作用示意图。
具体实施方式
为了更加简洁明了的展示本发明的技术方案、目的和优点，下面结合具体 实施例详细说明本发明的技术方案。如无特殊说明，本发明实施例中所涉及的 试剂均为市售产品，均可以通过商业渠道购买获得。
实施例1
本实施例提供一种沙门氏菌噬菌体，所述噬菌体头部长62±0.5nm，宽60± 1nm，尾部约35±1nm，呈多面体的头部和短尾结构，符合Caudoviricetes Podoviridae的典型特征。因此，该沙门氏菌噬菌体属于有尾病毒目，短尾噬菌 体科。发明人将其命名为：沙门氏菌噬菌体vB_SalP_TR2，并将该噬菌体于2021 年02月26日，将其保藏于：广东省微生物菌种保藏中心，保藏地址：中国广 州市先烈中路100号59号楼五楼广东省微生物研究所；获得的保藏号为： GDMCC No:61403-B1，分类名为：Salmonella enterica bacteriophage。
实施例2本发明噬菌体的分离与纯化
(一)宿主菌活化与培养
将-80℃保存的沙门氏菌甘油管按1:100比例加入到LB液体培养基中，37℃，200r/min震荡培养12h。经过沙门氏菌显色板划线鉴定后，挑取单菌落接种到 LB液体培养基中，37℃，200r/min震荡培养24h，制得单一的菌悬液。
(二)噬菌体的分离与纯化
25份土壤样品(广州市公园)经混合，浸泡在5L的超纯水中3～4h。然后 5000×g离心10min去除沉淀，上清液经0.45μm微孔滤膜过滤，收集滤液。在 滤液中加入固体无水MgSO4至终浓度50mM，混匀，室温静置30min，再用0.22 μm微孔滤膜过滤。取滤膜放入噬菌体洗脱液中超声洗脱10min，洗脱液用0.45 μm微孔滤膜过滤，得到噬菌体原液，并将其置于4℃保存。取一定量的噬菌体 原液与对数期宿主菌混合，加入含2mM CaCl2的LB中，培养24h后用0.45μm 无菌滤膜过滤除菌，得到噬菌体扩增培养液。采用点样法鉴定噬菌体扩增培养液中是否含有噬菌体，选择含有噬菌体的扩增培养液，进行下一步的纯化。用 接种针挑取单个噬菌斑接种到1mL目标宿主菌液中，37℃震荡培养4～6h，12000 ×g离心10min，去除大部分完整菌及菌体碎片，经0.22μm无菌滤膜滤菌后， 重复双层平板法。挑取单个噬菌体3～4次，获得纯噬菌体。最终在以Salmonella Albany作为宿主菌的扩增培养液中分离得到一株新型沙门氏菌噬菌体，该噬菌 体命名为vB_SalP_TR2(以下简写为TR2)。
实施例3噬菌体TR2菌体鉴定
(一)噬菌体形态鉴定
取15μL 1×1010PFU/mL的噬菌体滴加到铜网上，沉淀15min，用滤纸吸去 余液，再滴加10μL 2％的磷钨酸负染色5～10min，用滤纸吸去余液，室温晾干 之后，将制备好的染色片在透射电镜下进行观察并拍照记录。具体形态见图1， 噬菌体TR2符合CaudoviricetesPodoviridae的典型特征，具有二十面体头部(长 62±0.5nm和宽60±1nm)和短的尾巴(长35±1nm)。因此，此次分离的沙门氏 菌噬菌体TR2属于有尾病毒目，短尾噬菌体科。
(二)噬菌体的分子生物学鉴定
1)噬菌体TR2基因组DNA的提取
首先用PEG-NaCl法浓缩噬菌体，4℃静置过夜。次日，12000×g，离心20 min，然后用SM Buffer重悬沉淀。浓缩得到的噬菌体使用苯酚-氯仿-异戊醇法 提取基因组DNA，-20℃保存备用。
2)噬菌体TR2全基因组测序及全基因组特征分析
利用
3.0荧光定量仪对提取的噬菌体DNA浓度进行测定。取300ng 上述DNA，使用QIAseqTM FX DNA Library Kit建库试剂盒构建基因组文库。 质检合格后，在illumina nextseq 550测序平台上进行测序。下机数据经过质控， 滤除低质量的reads，剩余高质量的Clean data用于后期分析。使用SPAdes软件 进行序列组装和拼接，最终得到噬菌体TR2全基因组序列，见SEQ ID NO:1。 将基因组提交NCBI的BLATN进行比对，噬菌体TR2与肠杆菌噬菌体Salmonella phage FSL SP-076(KC139520.1)、Klebsiella phage Pylas(NC_049444.1)、 Escherichia phage Pollock(NC_027381.1)、Salmonella phage FSLSP-058 (KC139517.1)和Klebsiella phage KpCHEMY26(NC_049467.1)的相似度分别 为77.70％、77.08％、76.61％、80.60％和76.87％，覆盖率<95％，可以初步鉴定 为新型噬菌体。选择上述相似度高的噬菌体计算其与噬菌体TR2的ANI(Average NucleotideIdentity)，热图结果见图2，进一步证明噬菌体TR2具有新颖性。通 过与数据库比对分析，噬菌体TR2基因组包含95个开放阅读框(ORF)和6个 tRNA，其中32.6％ORF(31/95)功能己知或假定功能己知。这些ORF可细分 为结构功能域、核酸代谢功能域、包装功能域、裂解功能域、tRNA和其他功能 域，见表1。此外，在噬菌体TR2的基因组中，并未发现编码于目前己知毒力 或耐药性相关的基因，因此可以安全应用于食品领域噬菌体防控。
表1噬菌体TR2全基因组开放阅读框功能分类
实施例4噬菌体TR2生物学特性检测
(一)噬菌体TR2效价测定
采用双层平板法测定噬菌体效价，首先将噬菌体增殖液做10倍梯度稀释， 选取适当的稀释度，分别取100μL噬菌体稀释液和100μL对数期Salmonella Albany均匀混合，加入到5mL冷却至45℃左右的半固体培养基(含2mM CaCl2) 中，混匀后立即倒入已凝固的固体琼脂平板上，待琼脂凝固后，观察并统计噬 菌斑数目在30～300之间的平板，计算得到噬菌体TR2的效价为1.53×1010 PFU/mL。
(二)噬菌体TR2最佳感染复数(MOI)测定
按照感染复数分别为0.0001，0.001，0.01，0.1，1，10和100的比例，将 噬菌体TR2与对数生长期的Salmonella Albany加入到LB液体培养基(含2mM CaCl2)中，37℃，200r/min震荡培养10h，12000×g离心10min去除菌体， 上清液用0.45μm的膜过滤。利用双层平板法测定此时噬菌体的效价，效价最高 的感染复数为噬菌体的最佳感染复数。每个试验重复三次。根据图3的实验结 果，MOI为0.01时，噬菌体感染宿主菌产生子代噬菌体的效价最高，高达10.8 log10PFU/mL，因此可以确定噬菌体TR2的最佳感染复数是0.01。同时说明了噬 菌体TR2在较低滴度情况下，都能够对沙门氏菌表现出潜在的较强的裂解效果。
(三)噬菌体TR2的宿主谱
采用点样法来进一步确定噬菌体TR2的裂解范围。挑选了48株沙门氏菌(食 品中24种常见的血清型)作为宿主菌。将100μL对数期菌液与半固体培养基混 合后，倒入固体琼脂平板上，然后取2μL噬菌体TR2培养液滴加于平板的不同 位置上。待自然干燥后37℃培养6～8h，观察结果。试验重复三次。若平板上出 现噬菌斑，表明该噬菌体可以感染该株沙门氏菌，标注为“+”；若平板上无噬菌 斑出现，则表明该噬菌体不能感染该株沙门氏菌，标注为“-”。从表2结果中可 以看出，从Salmonella Albany宿主菌中分离得到的噬菌体TR2可裂解Salmonella Corvallis，Salmonella Newport，Salmonella Albany，SalmonellaKottbus和 Salmonella Istanbul五种血清型。
表2噬菌体TR2宿主谱
(四)噬菌体TR2对温度和pH稳定性检测
取1mL噬菌体培养液(1×108PFU/mL)于无菌EP管中，分别于4℃，20℃， 30℃，37℃，40℃，50℃，60℃，70℃和80℃恒温水浴锅中作用1h。待作用时 间结束后立即将样品置于4℃冰箱中冷却，经过适当稀释后采用双层平板法对各 个温度的噬菌体测定效价。试验重复3次，每次设3个平行。从图4可以看出， 噬菌体TR2在小于等于60℃环境下，作用1h后基本保持原活性；在70℃和80℃ 条件下1h，噬菌体效价下降较多，大约在103PFU/mL。由此可见噬菌体TR2 对60℃及其以下的温度均具有较强的稳定性。
用HCl和NaOH溶液调节LB液体培养基，使其pH值为2，3，4，5，6， 7，8，9，10，11，12和13。取一定噬菌体培养液，用不同pH值的LB液体培 养基调节其浓度至1×108PFU/mL，37℃恒温水浴1h，同样采用双层平板法测定 噬菌体效价。试验重复3次，每次设3个平行。从图5可以看出，噬菌体TR2 在pH 4～11范围内，其效价均在108PFU/mL左右，具有稳定和良好的裂解活性。 随着pH值的上升或下降，噬菌体的效价会迅速下降。当pH值低于4，噬菌体 效价下降一半；当pH值高于11时，噬菌体完全失活。所以噬菌体TR2能够适 应一定范围的酸性和碱性环境。
(五)噬菌体TR2一步生长曲线
以MOI＝0.01的比例混合噬菌体TR2和Salmonella Albany(106PFU/108 CFU)，37℃静置10min，12000×g离心2min，弃上清，沉淀用LB液体培 养基洗涤2次以除去未吸附宿主菌的游离噬菌体。加入10mL LB液体培养基(含 2mM CaCl2)重悬沉淀，充分混匀后迅速置于37℃，200rpm/min的摇床震荡培 养，同时开始计时。在0时刻和每隔5min取样100μL，0.45μm滤膜过滤，双 层平板法测定上清液中噬菌体的效价。试验重复3次，每次设3个平行。以感染时间为横坐标，感染体系中噬菌体的效价为纵坐标，绘制一步生长曲线，见 图6。从图中可以看出，噬菌体TR2的潜伏期为15min，之后在15～35min时， 噬菌体的效价显著增加，噬菌体进入裂解期，裂解时间约20min，裂解量为211 PFU/cell，且35min后逐渐进入稳定期。可见噬菌体TR2具有较短的潜伏期， 能够快速的裂解宿主菌，并产生一定量的子代噬菌体。
实施例5噬菌体TR2的防控及应用
(一)噬菌体TR2体外防控实验
用LB液体培养基将对数期Salmonella Albany的细胞浓度调至1×106 CFU/mL，然后吸取100μL上述培养液至5mL新的LB液体培养基(含2mM CaCl2)中。根据MOI＝0.0001，0.001，0.01，0.1，1，10和100的比例加入噬菌 体稀释悬浮液，充分混匀后，以200μL/孔加入到96孔板中，设定不加噬菌体 只加细菌培养物的试验组作为对照，空白组不加噬菌体和细菌培养物。96孔板 放置于Microplate Reader酶标仪中，37℃静置培养，每隔30min测定OD600nm， 设置6组平行试验。从图7可以看出，实验组的OD600nm值均低于对照组。说明 噬菌体TR2对沙门氏菌具有一定的防控效果，起到提前杀灭病原菌的作用。
(二)噬菌体TR2在牛奶和鸡肉中的应用
将对数期的Salmonella Albany用LB液体培养基调至细胞浓度为1×108 CFU/mL。牛奶样品中，抑菌实验分为两组进行，其中一组为实验组：取200μL 菌液加入到19.6mL巴氏杀菌牛奶中，同时加入200μL效价为106PFU/mL的噬 菌体浓缩液，将其充分混匀；另外一组为对照组：取200μL菌液加入到19.6mL 巴氏杀菌牛奶中，不添加噬菌体浓缩液，充分混匀；将经过以上两种方式处理 后的牛奶放置于37℃，200rpm/min的摇床中震荡培养，分别于0，2，4，6，8， 10，12和24h进行取样，取100μL连续梯度稀释后，用沙门氏菌显色平板涂布 计数，观察宿主菌数量的变化，每组实验重复三次。
在鸡肉样品中，首先对肉样进行预处理，得到无菌鸡肉。具体操作是：用 无菌刀将鸡肉切成表面积为3×3cm2，厚度为1cm的样品，用无菌培养皿装好鸡 肉，然后置于紫外灯下正反面各照射30min，得到无菌肉样。取100μL的 Salmonella Albany(1×108CFU/mL)随机滴加到肉表面，得到1×107CFU/piece 的人工污染样。超净工作台中风干15min，使宿主菌充分吸附到样品表面，取106PFU/mL的噬菌体浓缩液滴加于样品上，而对照组滴加100μL的PBS缓冲 液。样品在37℃恒温培养箱中贮藏，同样于0，2，4，6，8，10，12和24h进 行取样，装于含有4.5mL的PBS的无菌试管中，充分混匀，取1mL连续梯度 稀释后，用沙门氏菌显色平板涂布计数，观察宿主菌数量的变化，每组实验重 复三次。
从图8和图9可以看出，实验组中沙门氏菌的菌落数低于对照，且在牛奶 样品中效果十分明显。说明噬菌体TR2可应用到食品样品中，实现对一定数量 沙门氏菌的防控。
最后所应当说明的是，以上实施例仅用以说明本发明的技术方案而非对本 发明保护范围的限制，尽管参照较佳实施例对本发明作了详细说明，本领域的 普通技术人员应当理解，可以对本发明的技术方案进行修改或者等同替换，而 不脱离本发明技术方案的实质和范围。
SEQUENCE LISTING
<110> 广东省科学院微生物研究所(广东省微生物分析检测中心)
<120> 一种沙门氏菌噬菌体vB_SalP_TR2及其应用
<130> 2.20
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 70823
<212> DNA
<213> vB_SalP_TR2
<400> 1
cgtgctgtca gtatcaactg acgaacactt tttaataggc tggtagcaga gatatagttt 60
ggtttctcaa cataatcgta atcatcttga gccaaccaaa cagccatgct taaagctatg 120
ttgcttggat tggaatattt catgattacg ccttcttatc agaagggata ccgatagatg 180
cattgagatt gttgaagtcg attgcaggca atgctgcacg aactacttcg atatctgaag 240
gaatcagttc tgctgcttca ggccatactt caactaaacg tttagtagag ttcacactgt 300
aaactacagc tttaacgtta gctttaatac ttttagcact ggaatctaat tcctctacta 360
ctttctcggc atcaaggaat ttttgtacca gaggattatc tgcaagcaga gttggtttat 420
cgttattaga tttacgaata aatcttaaac cttcaaactc tggcatagac aatacagaat 480
gctttggata tgacacagtc ataccagcaa gattcatctt gtctgcattc caagtactat 540
agtctcttga gcttttaacc aagatacctt catcttttaa tgctttgatt ttttcttcaa 600
tagtattgaa gcggtcaact aaacgacgat acggttttaa cccaccaaat gcagcaatta 660
agcattccat cttaatcttt tctacattgg ctctggcttc ttccagacgc ttagggaaac 720
cagatttctc cattgcagca gcaatgattt tatcacgagc atcattagta agacgtacag 780
acatattatt cttccttaat ccagccgagt gcttcggcaa tagtaggaaa ctgtgatttg 840
aaaatttctt tagcagctaa agcaatttcc atatgttctt tttgagtgcc attagcactg 900
cgaagttgga tatacgtaat ccaactacga acattaccag tcatataaat tttggtaggc 960
gagcacattg gtaaaaccat acgagcacat tctttagcca cgccatgaga tagcaactct 1020
tcatacagac gttgagtatc tattaggtga gtatgaattt tagcctgtaa atcagcttta 1080
atatcactag ggatattgtc taccgagttc tgacgattct tcatatcctg agaacgtaag 1140
tcgaatgtag caggtaactc agttacttct gcgtatcttt gactaaattc ttgaaatgta 1200
aaactacgat gacgaagaat ctgtgctgaa atagcacgag tagtttcaat ctcaagagtc 1260
atgtgtgctt gttcaaacac actccaatga gcatgggcaa tacaatattt aagtaagcct 1320
gctactgaag gattttcttg attatcagga ttggatacac gagcaatata tccaataatt 1380
ttttctgcat ctggtgttgc agtaataaat ttgacctgca tttttactcc agtggtaatt 1440
tcaattgagg acttggcata gcagactctc cagctaatgc gaagtaagct gaaccatcta 1500
catagtcatc aagtttgaat gagcctcctg aggctcttgc agccttcagg aagaccataa 1560
acatccaacc ttgttcctca gtaaggttca ggttatacat tgcgttaaac gctgctacag 1620
tacgtgccat tgaacgttca gattcttggt cacgctcact ggctcggtca ccaatacaac 1680
gtttagcttc ttgcaaaatt gctggagcat tatcaataga ggtagcaaag gtcatttttt 1740
atatttctcc gggtgaagat acttatccaa tcctacggca catgtttcta atatttgatt 1800
ctcatcagcc ccgttagcca gagagatatc attagcccat gatggatgga acacactaag 1860
ctcaccccct aacttcactt catcgtgata tatgtcagga tggtcttgcc attggacaca 1920
ctcaaccaga ttgtcattca cccatttcac tacctcgata tcatcgggaa ccagataata 1980
ctgagcatca tgaatgtgtg ctgctggacg gattacattg gcatacttag atgcccatac 2040
acgttccata aactcattgg ctgctctgtt atttaacaga caccaggatt gtcctaatgc 2100
gttaccagca gtcctacctt cagctttcgc ttcataaggc atgttactgg ttccccatac 2160
gactttaccg agtaatggag tacgtattct taatccgaat gcagcagtga tgtaaccatc 2220
tttagttgct tgttctaact tcttgtcaac ccattggtca gagacgaaat aaagttcgtg 2280
atatctctgt tcaacagatt tagctttctc ttccgagaag ccgcagttag ccattaatgt 2340
gaaataagta ccttgatagg taagtgcaaa agtaggtgct ttagactcct gtctaaagac 2400
tgggtaaagt tcctgaatac tattgatgct atctacggta tcaataatgt caggcatctg 2460
agagccaaaa taggcaaatg ctcgtagact atgaccatca taaccatcgg tatatacctt 2520
cagtttattt gggtctttag ttgtaagagc agaaatacgg tcttccaaac tagcaaagtc 2580
caatccaatg agaagccaac caggagcagc tttaaagcat ctctttattg gtttggcata 2640
tggactcttg gatgatggaa tctgctgcat gtttggtttt gaagaactta atcgtcctga 2700
tacagtccca ccaatgttga agctaccaaa tagatagtgc ataccatctt ctgctagagg 2760
agcttcctca aatgcaggga tgaatgcaga aagtattttc tctactttgg aatacttaat 2820
cagacattcc agaatagatt tatacctttc gttatcagta tggtttatca gtttctcaag 2880
agtttcagcc ccagttgctg ggagcttgct ctctgtataa tcaatcacag gtaatcccat 2940
aatctcatac aagagttctt gtacttgttg atgggaattt ggattgaaat ctatagccag 3000
agtttcttca tcagatagag ttatctgctt agtcttgaga gatgcattac gagtgtctac 3060
atgattctga cgtagtctat gaattaacac atctactaca gagaacactt taatagcgtt 3120
tatgtagcca tcacgttctt taatcattgc tgccttcagc tttttaactt ctgccatatc 3180
aataggcata ccagttaatt gcatctgaat aatgttcttc agagaaggaa gcattagatt 3240
gttatagata tcccactgct catctaacac catagtgtca gagtgtttgt tggctacata 3300
ccaagtactt agacagtctg ttaagttgta cttaagcaaa tcatctatat cgattttagt 3360
aatatcatcg atatcgtctt gggcatagtt gccagcaaac tcatgagatt gagcttttag 3420
actaagttca tttccagcac acgagtttgt tgctaagtaa gcaataatct tagtgtcatg 3480
gaagtttctg cacattaggt gcagtccctt gagtaaacct tcttggtcta atagaccttt 3540
catccataac tgatggatta gaactgttaa gtcataacct gcgttatgcc aaatcaattt 3600
gcctttgtat gtctcaaaga aattcctgac taagccacga atcttctgag catcctcagg 3660
actgttggtg taatcgatag cgaaagcacc accattgtgt tcatcccatg caaaaccaat 3720
agtacctaac cctgctttat aaaacttcaa atcgaatgct tcgatatcag cagcaaggat 3780
tggatattga tgcagagagt ccagccaggc agagatttca ttgacaccct tgggatagta 3840
ttccgaatga ataatatcgc ctcctagctc gttgtaggag ccagaaatgg tatcggtgag 3900
agcttttaaa gctaggtcaa ttttgccttg tacagatggg tcataaaata actgaccata 3960
gttagggcag agaatgacat tgatgtgttc aaacccaggg attgcacaag gtagtacata 4020
cccatagtag ggttcagctt tctgcttctt ggttaacgtc ttgaagtatg ctgtatcagt 4080
acacagaata tattctgtac gcagattgca tagctctggt aagacttggt taaggtagtc 4140
tttctgtaga gagacaggtg ctttcttccc gttatactcc aagtcgaatg cgataaacat 4200
cctcttacct aatccggtag gagtgatgta atgtttctct aagtcagctt tcctaagttg 4260
ttgaggctta atgagaatag ctatagggaa ccttggagaa ggttcaaata tcagatgtcg 4320
catggctatc ctattggttc aggtggctcg tcagtaagat tgagaagcct atgctctttc 4380
cctgaatcta tcaactcatg aattaaatct attaactcta ttgatagagg tctgtgttca 4440
aggaaataag tcctttttcc ttcgtggtcc atgaacctac attcatctaa cattgcaggc 4500
caatggcctc tcaatagttt gtactcaact aagatagtca aaggtacgat gaatacatgt 4560
tccattatcc caccagacga aacatcacat ggaaatccat gatttgttta gtttcgatat 4620
ccagataaac gattaaacga agatgttggg gagttctttt aggggctgta gcaaacatac 4680
gactgtaagg ttcatgtaga tagtcaggaa tgacatcata catctgtccc caatccttga 4740
gacgagccat tactctggtg agatatgcat caatcttacg tctatcatta gctagcttag 4800
atttagcaat ccagatttct tctgcacgtt ccctgagttc ttctttaata cctatatgtg 4860
gagcattatc cattagtcca aatgatttac catcataggt aaatccaaat ggggtatcat 4920
tatctctaca taaagatgtt tctaatcttt gaacgtaatc tgaataagct tgggtaagtt 4980
tcataactaa ctccttcttt atatagatac gttcttgggt atgttgtctt ataggtatag 5040
ccattagaga ctcccgtaaa acaccacacg agttgttgca cgactaactg ctacatacag 5100
taagcgagct aattcttgtg ggtcacggca tacacgtaaa tcacttaagt ctacatagac 5160
tgtatgatac gtagagcctt gagatttatg agcagtacct gcgtatgcaa tacgtaagtc 5220
agcaaagtta ttctttaaat agaaataggt ttcccaatct ttaacatgtg ctgcttgtcg 5280
aatactccca gctaactggg tataactttc agcaacccat acatacagat tgttattcag 5340
aactacagtt ctaaattgaa tacctgattt gtcatctgtg tatacttctt tactaacaga 5400
agtaactaaa tactctttct caatcttggt tctagatgaa ccaatctcaa tagaattatt 5460
agagatgact ctatcaccaa ccattaaatg gattggtaat cctttttcct gacggatgta 5520
cgtattgaat tcttgggtac ggttgtttgt ataagcgagg attttgtgtc cgggagtttc 5580
ttggttaata aaatgtgcat ccatgagatt acgcatttct acgtcatcaa caaagtcgat 5640
gataccagga actggactaa ttggtttgaa gatacccgtt tcaactgttt ctcttaactg 5700
ggtgtttaga gcagtgagtg ctggactatt actagtacgc acttgggtag tcaaatatga 5760
ggtgagaaat cctgaattaa atgcaggact ttctttttct gagactggag ctagctggca 5820
gtggtcgcca ataaaaacta ttttacagtt tctatttgta gcttcctcta tgagcttcag 5880
taattgagta tctgccatag atgcttcatc cataaggagt aaagtatctt ggagatagga 5940
gaaggactta ctttttacag tattggtttt acctgtacgg taatcatccc ttactgttaa 6000
tccgattgtg gagtgaatag ttttcacatc acgattaggg aatctttctt gtaatacttc 6060
tgctgcctta ttagtggtag cagtaatagc aatatccttt agtggctctg cacccatgat 6120
attacagata cgttccgtgt tggggagcat atcaatcatg tggtcaagca tatatgtttt 6180
acctactcca ggaggaccag agatacacat atgtttatta ggagacacaa ggaaggctag 6240
gaaggtctta gcagccttct cttggcaatc agtcagttgc atcctctgtc cttaggaaat 6300
ccaatttacc aacgtcatac atggtcctgg ataggaacca catctgaata cgtttatcca 6360
ttggtttaac gacaatgcgg tctgcacctt taccatagtt acgagtttct tcgtacatct 6420
ctgcttcaga taataacctc agcagttcct gccctttttc aacaggtagt acgattttaa 6480
aatcataacc aagttcgatg cagaccatgt tagttgaatc tgtcatatac acctcagggg 6540
aagtatatgg accaatccgt tttaacgtat gcatcaatgc accagagtat ccccaggatt 6600
atgcacataa catagttcat ggtttacctg ctttattgag aaactcgttg acgatatcaa 6660
caggtgattt tgagtaatta ccaattggtt ggtcataact catatcacgt tcagctaact 6720
catcgagttt agctgcattt ctgttgtagg catcaactaa gtgttgagct actgtctcca 6780
taactttctg tagatagaaa ggctttgatg aacgtaaagc tctatcttct tcttcggatt 6840
caggagcgaa catgaagtct tgaaagatag gtagaccaga ttctgtaaaa cgaattgtac 6900
cgaatttttg gattttcatt tgagcaccta aacagtggaa tataagcaaa taaaccaccg 6960
aaggtggtct acttgatttg aaccatagtt aatcgtccac cgaatactgc tccggtatcg 7020
atatagtttt ggttcttaaa ctgtttgggg tctttgacag gagtatgacc gaagtaaaat 7080
tcatcggctc ctgtaatgac tacgctacga ctattctggt ttctaaccat tctatccctg 7140
ctccagacta cgtctgtgag gtttactggt ttaccgaatg tgtaggtatt tgatgggtag 7200
tcagcgtgac caatgacaac ttttctgtca tcgatagtga cttcaatgag ataaggtagc 7260
agagctactt tatgaaataa ctcaatggtt ttatcgattt gttcaactga ttgttcaaca 7320
aaccaagtac caccatttct aaaccaatgc gcgaatcgca tatctgcttc agcagtattt 7380
ggtttagtag taacagcatc gagagccatg atttcatggt taccaataac tgctttgaac 7440
catggttgat tcaataactc taagcactca atgttatgag taccacggtc aatcaaatca 7500
ccaacagaga taagtaagtc agtttgagta tcgaactgaa ctttatacat ctcttcgttt 7560
aaatctaacc agcagccatg taagtcgcct acaacgaaga ttcgtttgta ttggctacca 7620
tcgatagctt gataaacatt ttccatacat acctccaatg gtaactggaa gtactccccc 7680
aattaggggg agtactccca agcgccactg ccggtattag ccgacaacgt gttcgtgcat 7740
ttcagaaaca tcgcttgcaa tgcgtttaac gccttctgca attgccggag caacagcagc 7800
agcgataact tcattgaagg tcggttcttt cactttcggc ttagactgtt cttccagcag 7860
cttacgctga gcctggatag tcagagccga atccagcaga gcctgggacg agatttcttt 7920
aatcagcgta cctggttcct ggtactgcaa ctggtgcagt tttgcacgtt tgacgacttc 7980
agcgattaca gcaggaatag tacctgccag cgcttcacca accagagtca ggtcagcatc 8040
agccgcaatg gtatcgcgac catacagacg aaccagtttt tcaactgctt ttgcatcagg 8100
cgcggtaacg tcaatgattg catccagacg acctgggcgc agcattgcag ggttgatgtt 8160
ttccagatgg ttggtggtca gcaccgtgat gatgcggctg gatttggtgt cgataccgtc 8220
gaggatgttc aggatgtcat ccatcttaac ggaacgttca cccgatacag cacggtcgat 8280
atcttcacag aagatgacac acgctttatc gctgtattgc ttggcgaact gaatcgcatc 8340
gctcagttcg tcagagcgcg gtacgtagac gtaggtaaca cccacgttgg tagccagggc 8400
agcagcaaca gttgcagcca tggttttacc agtaccgtat ggcccaccca gcagaacgcc 8460
gcgtttgact ggcatatcgt tggcgataca gtcggaaacg cgttcgattg gggtgaacag 8520
gttagtttcg atgaggtttt gcacattgtc ggaataaacc agagaatcac ggctaatgcc 8580
gttcaggttc atgaattccg gctcaggcat ttccagcagg ttgccatcgt tatcacggaa 8640
gcgaatttta atcgctttac ccatataaat ggagttggtg cgcagaatct tctcaacagt 8700
atcgaacaga agttcgatag ttggttcgtc tttgcgcagc actttgccga ccagttcgaa 8760
actgacacga ccatcttttt tctgtgcaga acactgcacg aaaccttcta cctgaggcag 8820
agagaaacgg ccccatggta cttttttggt ctgaccgtag ccgacctgga catccagcat 8880
ctgcggaggg ttagagccaa agaaaccagg cgttgcttca gcagcagccc agccgtaacg 8940
ctcagtcaga gccagcatca gtgcatgagc accgtcccac gggaatacgt tgtaggtacg 9000
acgtaccaca acttcttctt cttcgtaggt ctggcgacgc ttcagcaggt cgattgcatc 9060
gcccagcttc atttgttcag ggacaatcag cttttcaccg tgcttaacaa tatcagcaac 9120
gtgaacctgt ttttcattct gttcgagagt tttagccatt actatttacc ttttcagatg 9180
gggattaaaa ttaacgaatt gaagagaatt tatttctctt tcttttttcc aaggcaaccg 9240
ccggaggcgg tttaacctct accgaacaca ggaataacac gatagttacc attgcgagca 9300
atgttagcta cgtgctgttt cttggttcga caaacgagtt tgttagggat acggaagata 9360
tcccatgcaa taggagattt ggacacttta ctcaccaaat ttaagtttac tttggaaggt 9420
actgtgtttt gcctcttctc tgttaagctt gccgtcttga atgaagtact gggtgattaa 9480
cgtattaacc atacggatac ccagagtgtt catttcatag ttctctcgaa tatgtttcga 9540
gatatctgcg acagctttct cacgcttaac atctgagaat aaatccagat acatagcgag 9600
agttttagat tctttcagga tattcaggag agtatccaga ctgactttag tggtgttgta 9660
taccaaacca acacgaccaa gaaattcagt cttcacgcca aaagcacgga gtttatccag 9720
attgatatct ggttctccat tgaatgcacc agcaaataca aacaagacgt tcttaacagg 9780
aacatctaca tacttgccat agtcgccaaa cactgaggta cagtctgact caagaatctt 9840
aaggaattca ttctgaactc cattggtaga ctcatgagcc agttcactat tgttattacc 9900
agcgatgaat aacttatcga attcatctac aaagcaaaca acaggattac cattggcttg 9960
acgtaatggt gctaaggctt tactcaggct attacctgac agaccttctt tggttagctg 10020
agcagcattg atttcgatgt aatccatctt atgttcatca gccaacagct taatcaaaaa 10080
ggtttttcca gtaccacttt cacccgttaa aataaagtga ggacgaagct cacattcact 10140
agctttaaag atggaaaaga tacgggaaat atctttccta agctgttctt gctctttcat 10200
taacaccaat cctctttgga cagtttaatg agttttcgaa tatgccaagg ggttttatcc 10260
tgtagcttct tcttacctgc taataaagca tcgttaatac tgtcatactc attaggagag 10320
acatgacgta caaaacgata gtcgtcgtta atacacattt gacctaatgt gaaatcaacg 10380
agggaaccat cagccatgag attcacgaag tgaacagtgc aacaatcatc ataaataatg 10440
actgcttcta ctacaccaac agccatattg tttttaacag cctgtacagc gttgtagtgg 10500
catctgtgat tagacagagg cagattacca gccaaactca ttgagagctt tctagggtac 10560
tctgcgttcg ctatatcacg gatagctttg cgaatcttaa acatgattca ctccaaatat 10620
tttcggaata cacctgcgaa gcaggaatct tcatcttgaa gtccatgctc ttgacataat 10680
ccatgatggt caaaacgaca atcatggcct acgtaatcta aagcatcttg caaggcttgc 10740
tttaaatctt ctggtaattc gaagtctggt tcaggttctg gctgtttcca tccacaacat 10800
tgacagtcca taccatcaac atactgacca cattcagtgt tttggcagta caggtacaaa 10860
cccatatagc ctccaaaaaa gatgccccca caaggagggc aaagaacatc tgaaaggtaa 10920
ttgaattaat tacctaatcg cggtttaaga cttaatatga attaactgcc caaacggaac 10980
tgttgcactt gggttatcaa gacacaccca aatgacaaag tctgctggtt gttctctcat 11040
aggagcacac tccaagtcag agaacacaac catgatagtt ggcttatgtt tcttggcatg 11100
tgctaataca caatccaaag atgtaccgcc acgacctgta aaggtcagtg atttgatatc 11160
catatcagga gttacttcat actcatcatg aattcgagta tcgaatgtca caatgctcat 11220
cttctctggc gttaacatct gctgaattgc gtgcatttca ctgatatata cattgaactg 11280
ttcatcagtt actgaacacg aaatatcatt ataaaaaatg atgtgagcca gagattcaga 11340
gttaagggat ggaagcatga tgtcagggaa gaatctctta ttgagtctct tccaactata 11400
gtcatccttt gccatctcat tgatgaagtc tctgaggata taattccagg gtaactttgg 11460
atttaggagt tcatccagat aagcttgaat gtctccagga actgagcctg cttgacctct 11520
ttgcttagca gtaatcacgc cacgaatgag catattgtca atttgagcat ctacttcttc 11580
ttctttatcc cctggtggag gattaacatc cccagccata gggttaggag gaagttgttc 11640
agctatcttc atcaactcat tatagatttc gtagacgttc atgtcgtcgt agtcatgatt 11700
gactagccaa cctggaccta atacataccc ttcattatca agtatattgt taatgaagaa 11760
gtcacatgca tagttccaga ttttagggtc cctaccttca tgtctcgtag gatgcttaag 11820
caccacatgc caagtctcat gagcgagaag tgtcactctg atttgtcttg gttgagccat 11880
aaagaattca ggattaatac gcagatttag accatctacg tcagcagttg gaatgtcatc 11940
acaccatgaa tgctgaagtg agcaaaacac tgatgtaata aatgctgtcc ctttcttagc 12000
aaagaaatca attttagcaa ggtcatagat tctttctaaa tccatagacc ctcctgttaa 12060
agcatacctt taccggcatc gctagcgtaa cgagataccc agtcagctac tgcctgaaca 12120
cctacaatcg ctggtttagc tttgaacgca gatgacaacg ttacaatctg gaattcagat 12180
ggcaaacggt tgacgaattt catcaactgg tcagcgttat ccacattaat actggaagca 12240
attaagccag catacgcaaa gcgaatgtct gcacgagtag gcacagtaat gttttctgga 12300
ttagcaataa tctcttcgag actatgtact tcaccgaata catcacagaa agctagaaac 12360
tcttgtgcag cacctgagcc gataatacct gccaatactg gctggtcttt acctgaatca 12420
gtaggaagtc ctttaatgta cttactactg aagtcccagg ttcgtggaca tgggaatgtt 12480
tcacgcaggt tctgaggatt gaatttatgg aacatttcct ttttaaagtt caaataaccc 12540
agaacacggt aatcaatacc ggatgcaata gcccactctt cccattcagg gaaatgaggc 12600
tccaacacca tatgcacgat acgtgacatg gtcgcagaac tcattgtagt ggtaatagca 12660
ccatcggtat caaggttacc agccgcagca atatacacct gaggatggag tttggtttga 12720
ccaaccatac catccaacag tttataagca gcagcttgaa cagctttcgg tgcagtgttc 12780
atctcatcga ggaacagcaa ccagccgtta taaccttcag gaatttcatc ccctacaatg 12840
gggatatctt ttggcgggtg ataaacagaa cgcccacctt cgatgttcgg gaagcccagc 12900
aagtctgtcg ggtcacactg acctaaacga aggtcaatta ccaacagctt agcttccttt 12960
gcaatctgac gaatgattgc tgatttacca atgcccggag agcctttaat aaaaggcact 13020
aatcctgagg caaagcagcg ctctatagcc tttttaatag cagatggacg catacctatt 13080
acctttcaaa agaaacgctc cgaagagcgt ttaagagact tttttcagac gacgtgagtc 13140
aaacaagaat ggtgcaatat caccatcttc ttcgcgaacg aggatgatac agtcatagcg 13200
tatagcgcca tgtgtcccac ctatagtacg taaaaggtct tcgccttttt cgtatccata 13260
gaatggacga gctagccatt cagcaataat ggcaggttca tcttcagtag gaaaacggaa 13320
gcaaccaggc aggtcaggat gtttaatgac aatgtcacca acattcagag cttcttgttt 13380
gaccaccatg gcatcaaagc cacgacgaag agcgtcaacc ttatcagttt caattgacat 13440
cttttttacc tctaaacatg ggccagagaa ttgctgcaat acccgcagca agtacgccgt 13500
cagctaacat cgacatcatc ttaccagtga agtcgatagc cattacgagg aacagcagta 13560
cggcaactgc tatcccccgt aatttcatac ccatcttaca gaagggtatc cagacggatg 13620
ccagcccagc cagcaacttt ttccaggaac gctttttctt cagcgccgat accgtctttg 13680
tcttcagcta ctgcgatacc acacagcaga gcacgacgtt gaccagcagg gtcacctttc 13740
aggtcgttga attctttttc caggtccatc attgcaccac gatggaattt ctggaatttg 13800
ccaatccact tgttggtcat ggcctgagtt tcagaacgcc attctgcaaa cgtatcatca 13860
gattcgatga cgttttccag aatttcgatt tcttcgtcag aacaggattt gtcggcaaaa 13920
gcaacgccaa ccattacagc gataccagct tcaacggtat ctttgctttc cagtttgcca 13980
gcagatgcac gcatctgttc aactttctgg cttacttttt ttgcaccaaa accaaataaa 14040
cccatgagat ttaccttctt tcagatatta cagagaattg acggctctta gtgctgagca 14100
gccggatgga tgaagtacac gcctgctaca ccagtatcaa tggtgtatgg cgactgcacg 14160
taggtaactg ttggagcacg tttgaattct tgttcaaact tagcccagct aaccgctgcg 14220
ttttttagat tttcagcaat aatttcattg ttgaatttct gtttttccag gaagtcacaa 14280
acgtcagtca tgtactgatg acgttcgata cagtgacatc cttcatgacc acctgcggca 14340
tggtcagctt tgattttctc accgatatct tccagcagtt cacgaggagt gacttcctgc 14400
tgaagaattg agccaattac cttcactgca atagcaggca ttggaccatc gtattggtca 14460
acaatacgca tggtttccat catggctttc ttttcttctt tagttaaaga catcttattt 14520
accttcagat tgtttaagta attcacctaa gccttcagca aaggtgcgaa tacgtgcgtc 14580
aagttcctca agagttttga tttcactttc acgcacttct ttgtttatta gagacttggc 14640
taaagagcct aaggattgaa aataaccaac attactcagt tgttcagtcc cagctttaat 14700
accagttttt ataattttcc tttccaccag aacatacgta taggcgtttg actgaacgta 14760
gtacttatca cctaacggaa tacacattct ggattcagcg gcttgttttg ccatgctcta 14820
ctccaaaaaa taaagctccc ataaagggag cctatttatc caaagaaaac gccggaggcg 14880
ttaaacagga taagccttat caaagtcgcc tttgttaagg ttgtgtctgt tgagatattc 14940
tctcagacct tcctgttgtc gagctaaatc agcttctaag ccagcaattc tatcgtggta 15000
ctcctgtaca ttcttacgat gtacatcgag ttcctcttgg ctcatctgtt tattcattta 15060
acacctgtag aattaaatcc accttcgcca cgttcagtag cttcaaaaga atctactaca 15120
acgaattctg gctgcattac agggacgatt accatctgag caatacggtc accagggtta 15180
actgtaaaac tctctgtaga gcggttccag aggcttacca ttaactgtcc ctgatagtca 15240
ctatcaatga gtccagtcag gttacccaga accactccat gcttatgacc taagcctgaa 15300
cgaggcaaaa tcattgcagc atacttggca tcatccaaat ggatagccaa tccagtttca 15360
accagtttgg tttcacctgg tttgagtgtg aatggcattg ctacgtttgc acgtaaatct 15420
accccagcag aaccttcggt agcaggttta ggaatgcaca ttgcaggatt attgggagga 15480
agtaccatca atttaacttt catacagttc cctatattag tgttagacaa ataccactat 15540
taagtagcat ttgtttaaaa ctaaaagcct ctccgaagag aggctaatag attaccattc 15600
gtaactgaca cctgcaccaa agacaaagtc attttgggta gtaccagcta cagatgcttt 15660
ggtaacaaca ttctggttga atcgtgcaga agcaccgaca gcaatacctt gctcaccatc 15720
atatgaacct accccagcac caactgagaa tgaactattc tggcttacct gaggaatgtt 15780
tgccatagca gctacgcctg caataccagc agatgcattt ttacgattct catcaatacg 15840
tttatccata ttggcaaaac gttgattggt ggatttttca aaagcatctt gacgcttaga 15900
gatagattta ttggcttcgg tattagcatt gacctgagtc atatctgcat ctttaccatt 15960
cagcccattg atgccagcaa taccttgttt gccttcatcg cctttatctc ctttagcgcc 16020
gtctttcccg ttttcaccgt taagaccatc tttaccagca gcgcctgttt cgcctttatc 16080
acccttttca ccttgaatac cctgagcgcc gtctttacca tcaataccgt cacgaccatt 16140
agtaccattg gttccattta atccgtcttt cccatccttg ccatcaagtc catttacgcc 16200
gtctttacca ttggtcccgt taataccgtc tttaccggct gcgccagtag ccccagtatc 16260
ccccttatca cctttctcgc cttggatgcc ttgagcacca tccttgccgt tggtgccatt 16320
gataccgtcc ttaccatctt tgcctttaat cactgtggct ttaccatcga catagacttt 16380
gtctacatct ccttcaaagc tcacattgag agacgagtta ttacacgtcc aggcagaggc 16440
acactgactt acttgaacag agccagtacc attaccaaca actacaacat cattagagtt 16500
attagttgag gcatttgccc atgacattga ggccattaca aggccgacag ctaacaaaat 16560
ctttttcatg atttactccc ggccttagcc gattaggttg aggatttaat taaagaggct 16620
gcgtactcta acaagtacag ttttggagct acccaaatct ttaaccaatc aagattgcac 16680
cagaatacga atatcattat gaaagtaatg attccccata taacagttaa agcaatgact 16740
gctgcatctt gttcggtcgt agtacttctt tggtttttaa accaccatct gaatttggtt 16800
aacatccaaa tgatgtaacc aacaataatg gcagtagcag aaaaccaaat taaaaagctg 16860
aaggtaaagt tccaaatgag aagttgtttt aaaacttctg gaatttgttc tttaccaaac 16920
tgaatggcag catcagcacc agaggtaact ctggtaatga tatcggctaa cactttctga 16980
agttggtcat tcatctttgg ctgcctttgg attgattaca gaatttggaa gacagctaat 17040
ggaataactg tcactgcgga cagctttatc acttggagca tataataccc agcaagtaac 17100
agtcgcatcg tcatcgtgta cacgttgaat atctaccttt gcaaatgcag gattaactgg 17160
ttctgcacga gcatatttaa cacccagcaa aatgcagagt gctaatgcca gccaaagaac 17220
caagcggaat tctttctttg agaacataca agttctcctt tgttatagaa gtgagtgata 17280
tattcacgtc cagtccagta gatgactctg actacatcct gatattcact tactaagtga 17340
ggaatgagcc aacagtcacc tacccagact ttactgaatg gttggatgtt ctggcttgga 17400
atgtagcgga ctttgggttt catccacttt ctcctgcata ggattagttg ggtcttcaac 17460
tacataccag ttaccgcttt cagcttccca catgagccat tcatcccctg cttcagcatc 17520
tggagtaggg ttacgaccaa tgaaggaatg ttcaggagta tatgcaaact cccgatacat 17580
atctttgaag cgaaccaaat gagtatcgag tacttcatca gtacctgttt caatgtactg 17640
aacccaaatt tggtttccgt acaatccttt gattactact tcacgttctt catctggatt 17700
agcagcgaag aacataatca ttactctacc gatacgaatc atgatttatt ttcccatttt 17760
ggggctgctt ctaacatttc agcgtatgct tctttagcag cattaatttc atcctgacca 17820
tgggtgattt ctccccaaaa accctcaatc attttaatgg tggggtcttt aggaactaat 17880
acccatcctt caggtatttt gatttcatcc attagtccat tctcccgatt aacttcgctt 17940
cgccattgac cgtatacacg gtcatggctc gctcgtattg ccaaaattcg atatctatcc 18000
catctgtatt accaatacga tagacagcta taggatgctc tggctcttca cctggatagg 18060
tgagtaaacc gtctttaaaa gcagatttgg tttcagatgg aagcatagga gttccatact 18120
gatagacatc ccaaatctta tatccaaggt ctatttcatt cttggatatg tttacagcac 18180
gttcgacaat ctggggtatg aaccccagag agtctttgat acgtgattta ggaataacag 18240
agtagtaacc tgtcgggtct accgacagat ttacatgagt aagaatacca gccatgagat 18300
acctcaggag agcatgtagt tagagttacg aatatcttta gacaggtcag ttgagagttt 18360
acgtacaggt actcttttac caacaatttg accaatcatg caggacagca tgtggctatc 18420
agccatatca gcaaagatat caatatagtt ctgacgaact tcattcatat acagtgcatg 18480
acatttgaag caatcgtgga tagcaatcag tgcgaatggt ttatggttca gcatctgttc 18540
gatgatacga atcagttcac gcttaatcca aagaggaacc atatctacat tgctatgagt 18600
gatatgttca gcgagaacta cagataccat tttatgagct tcagcaagcc ctacaagacg 18660
tttaagcttc tcagtgtgta catcttcatc tttgatgtat acgtggcttg taccccctgt 18720
taaggcgcta taaacacgct taatcatatc gacatcgtag ttagctctac ggttcatttc 18780
acgcactaca aatccatcca cggagtggat gacattggct gcaagagata atccagtagc 18840
agtacctttg ttaatccagt accgatgagt gaaggagcta ttcagttcat taacttcaaa 18900
gctttcttca accatagaca tgactttaac gatagctgtg aacccatctg gtaataccca 18960
catatgctgg ttagcaaatg gttgccagat atcaatcagg tcctgacgaa gcatgtttgc 19020
tccaggtgct acgatattaa ccatctcgta gaacttctgg agttcgatag agttctctcc 19080
gaacagttta cggggattgg cttgagagcc atagaagtga gtcatggttg catctttagt 19140
ctgagcacgc tcaaacttat ccatcttgcc gtctaacaga gcaatcattg catgatagcc 19200
atctgtataa acatccttac gacaatctgg gtcaatcatg tttactgaac gagcagtaac 19260
cttacagcca gtcaacgctg ccatgcattg taagcctgat gctgtagcat ccatacctac 19320
cagataacca gtaggcttac ctgctttagt atcttcatat gcgagtgtac cagcatagaa 19380
ttgagcaggt tcatctgcac tatctaccaa actggctaac tgcattttgt tttgttcgaa 19440
ccaatttaag cgttcttcaa acaggtcttt atccagacca aaggagttag caatatcgag 19500
tttgatatac tgaaaggcag agaatttttg catcttgagt ctcacttatt tagttgagag 19560
gaattgaaac gatttgattt gaatacactt gagggttatt ggtttggaat ataggacata 19620
cattacattt gaaatcacat ttgtttgcga tttctaattc accaaaacct cctttacacg 19680
cactatagtc tattgaccaa atacgaagag atatgagaat ttcaattaat tctttattca 19740
tatcttcatc cttagtattt ggttcttata tagattaggt gaagggtttt tgtttagagg 19800
gcatgatgaa cataaagaat ctggtttaca tttgtgaact aaaagtaact ttccccctat 19860
acactcatac atgtaattaa ttctattcag ttgattaagt atgaataaaa gttttttatt 19920
catctgggat aattcccatc atcctccctg ctgcatctaa gccatagaca gtctgacgta 19980
cttcgtcatc agaacatgac agcaaagcac atttgctttt gtctctggtt tcaccatcaa 20040
cactgcataa cttcatgcag gctggtttag tagaacagaa atgctcttca agcttgtctg 20100
gtacttcacg aagtaacatt acaatcttga tgaattggtc tggtttagag agtgccattt 20160
gttattcctc aatggggatg agttctttat cagccaattc gagaatggct ttacgatagg 20220
tattaccctg atatgagaca tggtatccct gacaataggt acgaccacgt ttgtcatatt 20280
tatgggtaag atggaatcga ttaccttcat tgatgagcat agcgaataca cgcatagatt 20340
ctttttccat tttcaggaat gaagctacac gagattcata ctcatctttg gtttcatctg 20400
ctttaggtgc atcaagatgt ttacggttat cacgtatatt acgaataaca cgctcatcta 20460
aagcaaatgc aacagagtta aatcgattga tattatccag acagatatct tcttcatggt 20520
gattcttctt aagaattaag gaatcccatt caatggatag atatccacta cctttattat 20580
ctgtcacctt cttaggtgga actaacattg gaggtagata ctgatactga ttaatcaggt 20640
ccagtgtctt tggttcaaga gttactttag agacaagttc catagaacca gagttggaga 20700
atgatgctgg aatcacatca tagaggtctg caatagcacc cacagcaata agttcaccag 20760
cagtacgaat agcagcttta ggctccatgt taatcatacc ggataccata cccactactt 20820
gggtatattt ggcatgacca tgttgagcca atgaaccaac aacacttagg aagaagtcag 20880
tgaatatttc actgtctagt tctgccagac gttggttctt actttcaaag taatcccctt 20940
gttgccattc aattgctgct tttacagaag catcaacaag ctccatcaat tcactacatt 21000
ccattaattc tttacgaatc aggtcacgag cctgatgctt agaatacata gtttcaatca 21060
gaagttgggt ttcttgtttc atgattaaca gcttccatag ttaatgtcat ttgagtggtg 21120
aagtgctcac cagaagattt gtacttattt cttacaggac agttcttaca gtccaccatc 21180
ccacattcat agtcttcact ataaaattca tttattacac ggtcacaaag agtattggct 21240
ttcaccatat cttttcttaa tgaatgaaaa atgaatataa gttcattgtt caactaacac 21300
ctcaatagtt tttatgtatt gaatgtttat tgcaggacgg ttgtatttat aagaaatcat 21360
atttacaggg caacaatcgc attcaacacc ccaacaattt attagaagac agaagtggga 21420
taatattccc ccatttgttt tttgtaatgt ttcaattatt agaagtaatt ctttgtccat 21480
atacacctca ataggtgcag actacaaacc tgtcatccag taaaggttga ataatctttt 21540
tgatgtcttt ccattgaagt tctcctgcac cacatccggg acgtggaagt aatactttct 21600
tccatccata ttggtcagct aatttaacta gctgcttaca ggattgaaca atcaaatcag 21660
gcttagcttt acaagcccat cctggaattg ttgtacctgg ttcaaagaac ttatggttaa 21720
cgaagtttgt accatcactg gtttcaacta ctggtttaac tggaaatgac acaatccaca 21780
tatcattatg aataatgagt ggatgtacat tattcccttc tcgtgcaatt ttctgtccta 21840
ataccttgtc aataccaggt aacaggtcac gaatctgtcg agcaatacca gcacccatta 21900
cagatgcacc attgctttta acaaagccat tggtagtgat acagattgca tctgcatcca 21960
tttcaagcat gttgccttta gcttctaaca tggaaatacc tcttttaaag ttgcccagaa 22020
acagccgaag gctgaggagc gaagcgacaa ataatcaaat aggaatacga aataaaagac 22080
gagatacata taaatctgag gtaattgaac atgaatttaa aagacaatac tcacacccat 22140
tacctctaca tatatctcgt ataaagataa aatgacataa tttactattc ataaatgtat 22200
gcgatttaag atggatgagt atggtgatta aatcatggtt cataagcagg aatccttaat 22260
agcagtgctt gattataata agtagctcct gctatcaaac atactccaca agatacttgc 22320
cagcatttag ctggcttgta catattaaat aaaccacata actgacttcc tccattaacg 22380
gatttcatca gtctaattaa tatgaattca agttcgtggt tcataatacc ctcctagaag 22440
ggtatcagag taccaagtac aactgaaaag attgcatcac gtaaggtctt gcaatagcga 22500
gtacctacgt aatacatatt cttcttacct acaggtcgtt tgagaatata ggtggaagta 22560
ccattaatat ggactcccca taatttctct tcacgagagt aggacactga gtattcaggt 22620
acagtaccta atactggttc ttgtttttta gcttttgcca tttgagttac ctctaaggtt 22680
tttgattacg tcttgaatat cagacgcata gccatactcg tccttataca ttaaaggaca 22740
atttccacat gcaaaagaat caccatataa agcaattgca tctactttac atcttgggtg 22800
tattcctttt tcacacaata aatcaggagc aatgaattct ggttcttctc ctacctcagg 22860
agcatcagac attgactctc tgatagtgaa aagaataaga attaattgct cattcataaa 22920
ttacctaatt gaattaattg atacccatac catttaatat tagtagtaga tataggacat 22980
ttatcacata gtacttcagc acagttataa tcgattaaat tacatctact tataacagtt 23040
gattgaagtg ttttaagtat gaatattaat tcttggttca tatgttttta aatacttgaa 23100
ttaatttagt actgtatcca tcactattac tataaccaat aggacaatca gtacattccc 23160
actcaccgtt gtattggcat tcccatatac ttgttccacc taaattagtg catatattat 23220
ctgctgggga tttaaggtta tgttctttat taggcccacg taatccatct tggattacat 23280
taagtacttc catcaattgt ttattcataa gtaatatctt ttatttgaat aagtaaatta 23340
ggataatctg acatgaataa agtagatgta tttaatagac attcattaca ttcacatcca 23400
ttacatagat gatttatttc taaatcaata catatacttt tatatgcgaa tcctttagat 23460
attattactt cgataatatc tattaagtca ttattcataa ctgtctccat gtttttatta 23520
ttaaagatgt ataatattgg taatctttat atcctaacat acagtcgtta catgcaactc 23580
cttcacatcc ttcatccata taattaaata tatggcatag agcatctatt ggtgcatttt 23640
cctcacccat atgtctggtt tcagcaacta ttgcctgaat gattaaacct aaatgtttat 23700
tcatgactat ctctaagcgg actaatatac cctcctaaga gggtaagttt ggtttacttg 23760
tgaacagtga ccaacagaac acaataacga atccgattag ccctatcaca gtacatgcag 23820
cgaaccacac aatgcttaca gccattgcga agagttcaaa gagcacgcga agcgtgacaa 23880
caatagcaac aaagaataag ttcatttgat tacctcacag gaattattag ttaaacgaat 23940
agcactacgt ggaataccag atgatatctt gtctttaaca caagccatct gctcacggta 24000
ttgcactgca tcttcttgga ttacatccag agtcagtttg ccaaagcaaa ctaagaacag 24060
tagaaacact gctaatacac gcatacatca ccccataaaa taagggatac ccgaaggtat 24120
ccccatttga attagctgag tgcagacagg attgttgcca tggtgctgtt atcagcacta 24180
gcttcgacct ggtctttacg gcgatacaac tgcacagcca gcttaggcag cattttgcca 24240
gtaccagcgt ccagaccttc gcccattttc accagcgctt tcagaagctc gttacgagcc 24300
gcagcatcag cgttccactg ttcgttgtta ccacgaatta ccagttcgtt catggtatcc 24360
agcggcaggc cgaatggcaa agacagcaga atctcttctt ccaccatttc accatcacgc 24420
tcgacagtac gagtgattgg aacaccgacg ttgatgaaga attcagcctg aggacgtggt 24480
tcgttgccac gaccagcagg tttgccaccc agagcctgag aaatcagaga agatgcgttg 24540
ttagctgagt taagaacggc catgataaat acctcgatag ttaattgaat tggattattc 24600
caccagcgac gaagtcgctc ttacagcaga aggagaactt acggaaagct ccaccactgg 24660
cgaagccagt agcagagtga gattagtagc ggattacaat gacaggctta ccctttgctt 24720
tcatggtttc aatcatgtgc cttgtaccac gagatgcacc atcccagaag gcaacaagga 24780
aatcagcaaa ctctgccatt tcctcattac gtataaagcc acctctacga ggttcagctt 24840
tccagtctgg atagaactta tgtattttat tctcgtagat ataacgccat aagttatacg 24900
cagtgatatc agcaccacga gccataccac acactagttc tacatcatct ggcagagtgc 24960
cattaagaac tagattcttt aattcagcat ccatgcgact gctatcagta aagtctctac 25020
caccagctac tattaacttc attggttagt cctcacagcg aaggattgca gtcttcagat 25080
tgttgatggt gtattccaga aactgtttag ttatctggtt actgctcaga gttttcatct 25140
gagcaaatac agcttctatc tcaacaagct gagcttgtaa tttctctttg ttactcaacg 25200
gtaatctcct ctaaatattt atctaatgct tcttgtgctt tagaaagaac aagtctttta 25260
aggaaagcat taccaggaga tgtagcaaga ctaatgctag ctcttactac attctcgtgt 25320
agttccctaa gtttctttgt ttgttccttg gttaacattg gttcacccat ggatggttgt 25380
aggtaataat tcttttaggt tgcttagctg atacatctac acaaggtaga ttaagttctt 25440
tagcacgacg tttaataaca tcagctttag taccttgatg taatgactta cgcatcatca 25500
tctcctccac gagtaactac aagatataca acaagaccac ctacgatagc tccaaacaga 25560
cttggaacaa tctcagctag ccaccatttg agtgcatctg aggttaagaa ttcttgcatg 25620
attagttcct atttaataac agctacgata tgtttgaact caacagtgag ttcatcacca 25680
tcagacaggc catgataacg agtgagtctt aagtcctgat tgatacgtac accatacttc 25740
cagttatccc tgtcaatggc atatacaaca gcccagaact tttctgagcc tgacacagat 25800
tcattttcca tttgtacgcc aatcttgacg tagtcacctt cctgaatctg agatggttca 25860
gcaggtacaa ttgcttcttg tggaagcatt gaatggaaac cttcaagcag tacatattta 25920
gacataaata gctcctcgta acacccagtt agtaaccagg tgccacatag tattatttga 25980
ttggattggg ttagttagaa tatcagaggt attcttggat gagtttgact aatacatctc 26040
catgacatgg cttaggtttg cagaagcacc ctaatacttt gccatcgagt ttaagtaatt 26100
ccagatataa atcaggttcc tgatgtagtc tttgacgcat gtattgctca tacatctcaa 26160
tgcatagttc tctgccatat gcttgaactg tataaggatt tccccatata ctacctctgc 26220
cgatatagac atcgaatggt tccttatact tgttgactac acgagttgaa gaagtcttag 26280
gaactatctc cataagttgt ttgatagcca tgggttgata cctgttttgc tttcaccagt 26340
accctcgaag agggattaac atctcagatg tatataggac gaaaggaatg gtatctagaa 26400
gctcatagaa taccttaggt tagcctaccc tattacctct catcaaaacc tctcagagag 26460
cttcctatga gcttatagcg atatgtatat atgtacagta taaatgtaca tgaatatcta 26520
tctcatgtgt tatgtgtgaa ttgattttaa aacctactcc gaagagtagg cttagttagt 26580
tattcagcag gtttagtagt tgtacctaag tgcttagcta ccagtgcgtt gaatacagct 26640
ttaacatctg ggtctgcatt agccatggct ttggttgctt ctgcatgtac ttctgctact 26700
tctttagcag cttccattac atatttggta cggaagattt caccctctac ctcacgctta 26760
atagcacgat gatgacgaag gttatctact tcttcgttag cccattcagt aacagataca 26820
gctacgttag cagtattagc tacagctact gcaccacgtt caattgcatt caatgcagat 26880
acagtagagg aagatacagc accaaagata ccgatagagt taacaggttt catatacatc 26940
tccaatcatg attgtagagc accattgctc cactagagtc gaagactcat acatgtgtga 27000
tgtaatgggg ggggggtaca ttgcttaaat ttatttgtgt gccttatata atacatccgt 27060
acataattat taaattttct caaacctgag gaaaaataat ataagacatt caaataaact 27120
taatccccag ttaaggggat tagaagtaag taaagtagat accagtaaat actaagatta 27180
ctgctattag tacaaagatt aactctatta atagcctatc tttatccata ctgtatgaat 27240
ctccatcttg acagtagtta gttaatcttt atcattatct ttataggata cgtcaggatt 27300
accgtctgag tctgctttac cagatacatg tacattgata cctgtaatgt attttctggt 27360
ttctaatgca gagtagatga cgtccctaat catgttggtt cctgcgtgca tacccgcctc 27420
aaagtctttg ttcttggatt taccctcgaa gttaagacct cctataaagt ataggattct 27480
cttcatctgg gagcgtctac ctgatgccca gccaaggatg aagaagatta ctgctgctcc 27540
gagtaacata gggtcaatgt tcattggttt agcctctgcg agcgaaggtg agattaatgc 27600
ctggaaggtc cagattgtag cgggtatcag tatccgtatg gactgtatgt gctcgcatct 27660
gaagtgcttt ctctgtgcag tattctctgc gccagataag ccagttaata tcatcttctg 27720
tgttttgtgg attagggagt agttcgagat aacgagtagc ggctctacgc catagcttac 27780
gtttctccag ggatttggca atggggtcaa taaccattag aagctccgaa taagatgttt 27840
gttacagtga gtacaaacat aatgatactc tacggaacca tctttgaaac tatacacacg 27900
agaagtaagt tttaaagaat ggcccattaa aagacaaatg agcctattct tcatggctta 27960
ttccaaacta atagtgttac cacaacgaga acaaaccgct ttatgagttg tttcagtcat 28020
aaatggaatg taattgtgat tgccaggagc acacccagtt ttatcagatt taccatattc 28080
taatggttta tagacaggaa ctggttcgaa gttgtatgca gtagccatat ctacacggtc 28140
aaattgatta gacgttttat agacgtatac tgctttatct tgaaagtgag caataatagt 28200
aactacttgc ttttcagaat caatcttaag taagacacta ctgccaatat caggaagatt 28260
cttcatctct ttgatttccc caagagtcta ctgtttcacc tgaagagtag acgtggaagt 28320
tgatatatgg gacacaacga actatctttc cacagcattc tgttttagcc cagatttctt 28380
taccatagcg gtccatatga gcttctgctg gttcaattag ttgaagttgt tccccacaat 28440
gtgggcaagg tttcataagt cctcaatagc ccccgaaggg gccagtcagt tattcaatct 28500
cagtcatcag agattctttt ccgatgaata cctgagggtc atcccagcgg tcgacataac 28560
ccaatactgg tttactttgg aagtaaccaa tgagagtcat ctctttggaa tcaccatcct 28620
ggtagtacag gacttgtctt ggttgagaag aggccatttc gtctccgatg attaaacggg 28680
ctgcttggcc caaggtttct tgatgagttg aaattgattc aacgagttca agttctacag 28740
tttgagaacg ttcccaatta ccttcaaatc ttccacgagt agaagatgca tgttctacaa 28800
aggtaacttc tgaaccaata ggaaagtgat gttcgtcata tccattttga ccacgaataa 28860
tgcctttatc accagctttc cattcatctc ttggtttagt atcccaagta gctggattag 28920
atactggttt agcattagat agtggttcat cagtctcact tatagacaca tcgcctttag 28980
catcagtaac ttctgcatag aatacccatg catctttctc aggataaata ccaagagttt 29040
tccagttacc tgttgccctg acttcatcaa aagcatcttt agaatctttt aatccccaac 29100
cagtaagttt acgaatagct ttaatgattg taactgcttc tgaattacgt tctacataga 29160
attcaacttc gttgtgtctt tctggtttag gaatacgttc aagagtttta aactcaactg 29220
attgaacagt accatctttg ttagtgaatc caccaaaccc actgccataa tcaccattaa 29280
aagtaattac ttcatactta ttgaaacagt gaaccccatt ggtttgacga acgatagcaa 29340
ggtcaccctt gttccatctg ctttcttcag tgatttccca gtctacttct tcatttactg 29400
ggatttcttc atcttcagca aaagcatatg taacaatgtc tacaatagga tggtccatag 29460
acaccccagc atatttaata gttacatatt cactatatac attagtaata atgccttcag 29520
taccagcagg atgcccacaa gtagtattta tacgaatacg tgctttacga cctatttttg 29580
gttccagtgg tttgacttga tagaggaatg gatattcttg cttttcgtca tcttgggcaa 29640
gagcaaagtg agggcatgta gtaccgtcat catgaatcag ttcaataatc tcatcttctt 29700
caaattcaca ctcttcaata acaatgaata aatcaccttc tttataacca agagcttcag 29760
caggtgtttt accttcgatg actggggcta ctttgtgaag ctcaaagtat tcagtagttg 29820
tatcatcttc gtcatactct tcagtatcac gaaattcagg acaatcagtt ttatcatcgt 29880
aatgaagata aacaatgtct ccagcagata aattaccatc ttcattagtg agtaaggtaa 29940
atgcctgacc ttctgtataa cctaattttt gacatgggga ttgcatgtaa tgttctccta 30000
ctagcctgaa gcgaagcgct ccgagcgaac gtagtgagcg atggaagcgc gtaagcgtaa 30060
ggcgggatta gaatggggga taaaaaaagc ccctcaggga aaggggcaaa tggaacaaat 30120
caacgtgaga gatacacaca gcatctcttt aaatattgga tttacttaaa gagagactgg 30180
ttgcccatta ttaatcacag cgggccactg cgccaaattt tgttatgagg gattggaaga 30240
cctcactgat ttacaggctg ttaagccgcc atcagaacga catcatcgtt tgcatttatg 30300
tttttggttc gtttctaaaa aaccgcataa tcgcatacga aaactatcaa agagtacact 30360
gttgcaatcg tcattgctca caggtgggga ctcgaaccac cacaataccg tatggctaac 30420
gtcgctgtgc ctattcgcgt tctgtgcaat gtactcattg ataggatgtc taagctggtt 30480
aggccagcaa ctccctgagt tcggtaacag agttctagta ctctggaatc tctagtggtt 30540
aaacctttga ttcgtgtacg ggatataccc gttccgttaa cccaccgcta aatgcggcta 30600
tcttcggtgt cgtgctatgg cagcataact aagagaacac ttacacgcat ggttaagcaa 30660
catcagtcca tcgcccctgc aaatgctctc atagttatgc gctggtttaa gcagagccag 30720
cttgctatcc ccatccacgt cggaggacgt aaaagaagtg tattacttag aggtgttaac 30780
tacaacaccc tggcgaccag cttcggcttg tgcgattgcc agacgagttt cacgctcaga 30840
tttgatgtca gattgcagtt gtgcaacctg agcttccagc attttgattt tgtcgtcttt 30900
cgcgttggac ttagcagtca gttcagccac gttgatacga tgtgcagatt ctgcttcttt 30960
cagtttagcc tggaattcag cagcaccttt acggaagcca gcactttcag catctgcaat 31020
cataccagag taatcttcat tggcttgagc caaatcgttt tgcagagttt gcagagcatc 31080
gttggtgata tacgccaaac caaactgaga cagcagttgt ttgagtacgc cattttcgtc 31140
ttctttgata cgcagacgca gttcagcaga cgcttcacgg aatttgttat cgaattcaat 31200
atcgaggttg tccagttggg actgtttgaa ttcgatttcg tcagccagtt tgacagtaat 31260
gtcacccaga gcagacaggt cagaggtaat tttacccaga ccagcgatag caacgaccag 31320
agctttagtg gaacggtcag ctacagtaat aaccttttta acttcagaca tgtgtattct 31380
ctcagatttg ttgaaataga tttggggaat taaaaaagcc ctccggggat gattccagag 31440
ggcttagtgc tatttaccca tagctgggag gcttgctttg ttaactactc atcactagag 31500
cgaggatgga ttgataatcc agttaactta gcttcaggca cccgctggct ggcgagttct 31560
ttaagctggg aagaagaagt tcattgtttt ctcataacca gcattaactg tgtccatacg 31620
ttcaggacgg ttcatacgct tggagttttt gttagttttc tccatgcgac gtgcatgacg 31680
tgacgcagcg cgacgttctg aacgattagg acctttattc attttaatat aacgcatgtt 31740
atttctctct gacgttgagt ggtgggaata gtcggactcg aaccgacacg caataaagcg 31800
agggatttta aatcccttgt gtctaccaat ttcaccatac tccctttggt gctcactcac 31860
ggaatcgaac cgcgtccatc ctgcttacaa ggcaggcgtt cacccatatg aacttagtga 31920
gcttggcaca ccctattgga ttcgaaccaa taacctaaag attagaaatc ttttgctcta 31980
tcctattgag ctaaggatgc ttatcctcgt ttcgtcccaa ggtgaggttc cccttgttcg 32040
tgcgcatggc cggactgcca gttattcatt tataagtgct agatatgatg gtggaagcca 32100
caccttaagg aacacaaata aacatcccga ctgacaacgg agcacaggtt accctgtaca 32160
tttagatgaa caacatgata gtgattattg ccataatcct aacgaagctt atgcagtggc 32220
aagttgcatc ttaagccaag tgtcacttgt gaaaacgtta acggaattca cgaacacttc 32280
acatccccgt agatgcagaa agttttaaca atcatgctgt tcaatttgga gcggataata 32340
ggacttgcac ctatcagact gcgcttccac ttatcttaag ctaagtggtg tcagctatct 32400
cgtttagtac ccgcattatt cattaggaca gttgcaaggt ttaccccagt aaccacaata 32460
aggacattgg gttagtgggc atttaacagt aaacactttc atacatcctc caatacatat 32520
aagtagactc cgctccctca gcgatgaggt aacaccctta cggatgatag actaggagca 32580
gagtctctaa taggtattag tgtggggcat agaagatttg aatagcgttt ctcttcatct 32640
atgtctggtt tcgagatgac cacccatctc ctctataagt caggctttac cagcagcctc 32700
atcgtgtgtg cacttaccgg cgagataagt agcgcacttg aatggcggag aaataatagt 32760
caccacaaag ctcctcccca ggactttaca ctactactat tcccccataa taagtaccta 32820
gatgaaatgg gattcgaacc catgttttgt ctcgctactg cgttacagta ttggtattgc 32880
tttaggccgc taagctattc atcctaggtc tcctggctat tcttcccagc gtcagttgtc 32940
taatcccaac agccaatggt cttcgctccc atcgtcggca gattaccctc tcgcttaggg 33000
actcactata gactcaccat ttaaggatac cgtctataga ctattgtttg gcggaagata 33060
ctggattcga accagtgggc agctttcgtc accttccggt tagcaaccgg acgcctttag 33120
cctctcagcc aatcttccaa tatggtgtga gtggatggat ttgaaccacc gcgctgttac 33180
ggggagattt acagtctcct gcattcgacc gctctgccac actcacgtaa tatttaatcg 33240
actgatatct tttggaggac caccctcatt tcctcccatt tcgaccgtcc ggcacggttt 33300
agtggtacgt ctcttggctg ttacttggta ggagccgatt ctcccttgaa tgacctttct 33360
caatgaatgc cttcattgga tttcgcagtt gcagacaaag atatcatgcg attaaacact 33420
aacttttcca aagacagccc cgcaggggcg ataagaagaa tttcacttct tatgggcgat 33480
tactatatag tgccgataaa ctgattgtaa accccattgg agaataaaat ggctgaacaa 33540
cttactatcg ctcagttcaa gcaagtaatg cctagtaacc acaaaggtaa tattaaccaa 33600
gaactgatgg ataagattaa taaactcttg gctgacccta atatgggaga ggtattgaga 33660
gataacttaa tgtcttacac ccatgtaatg aaagagggta gattcaagac tgaagactat 33720
atctctgctg ttaagtatgt aagttataag ttaatgggtt ccactaactt tgaggcttac 33780
atcaaaacat tcccagaccg atatcaaaac ttccttgata acaatgtacc tactaaagat 33840
atccactgtt acgtatctgc gtataacaag aacaagttag taaacttggt ttatgaacag 33900
actttaattc ctactcatgt tctgaatgcg gatatctatc agaaagctat taacgttcaa 33960
gctgatttga tgctgaatgc taagagtgag aaggtacgta gtgatgcagc taactcattg 34020
cttaaccatc tgaaaaaacc tgagactcat aaagtagaaa tgaatatcgg tatcaaagaa 34080
ggtggtgcaa ttgaagagct acgagaaatc acttcacagc tagctgcgaa gcagcagcaa 34140
atgattgagt ctggagtata tacagctaaa gacgtggctc ataaaccact gacaattgaa 34200
ggagaatctg aacgtgtcta agattaaaga tgacccaatg gtcaagaaga cagtcgaaga 34260
atggctgaat tctgttgact acgcttatct taatgaaggg aactatgttc catctgtatt 34320
ttcacttaat tatgtgaact tcattaagtt agttaatggt gaacaggggg aatctaacaa 34380
gactcctgta gtacacctta aaatgcttga tgaattagca ggtaaacgta cacgtttagc 34440
taatctttgt tctcgtggta tggctaaaac caccctgttt ggtgaatatc tcgtactgta 34500
tattgctctt tatggtgaga ttgagggctt tggtaaaatt gaaggtatga tttatgtgtc 34560
tgactccatg gagaacgggg tcaaatcact aagaaagaac atcgagtatc gttataacaa 34620
ctctgaattt cttcagaagt tcattcctga cgctaaattc acggatgcct acctggaatt 34680
caccaatttg gatgggcata agttaggtgt tcgtatgttt ggtgctaaaa caggtcttcg 34740
tggtacgaaa atctttggta aacgtcctgt tttagcggta cttgatgact tggttagtga 34800
tgatgacgct aaatccaaga gcacaatgca ggcaatcaag gatactgtgt acaaaggcgt 34860
cgattatgca ctcgacccta cccgtcgtaa gattgttttc agtggtactc cattcaataa 34920
gaacgatatt ctttatgaag ctgttgaatc tggaggttgg catgtaaacg tcttcccaat 34980
ctgtgagaga ttcccttgtt ccaaggaaga tttcgtaggt gcttgggaag accgtttcga 35040
ttatgacttt gtcaaaggtc aatatgacct tgctatagca actggtcagg tagccgcatt 35100
caaccaagaa cttatgttga ggattacctc agatgaagag cgattggtac tggattcaga 35160
gattcagtgg tatagccgta agagcttatt agccaacatc aataacttta atatctatat 35220
caccactgac tttgctactt ctgagaaaac tagtggagac tattcagtta tctcagtatg 35280
ggcattgaat agtaatggtg attggttctg ggtcgatggt atctgtaaac gtcaattgat 35340
ggataaaaat attgatgagt tatttcgtct atgccaactt tacaatcctg taagtgtagg 35400
gattgaagtt acagggcaac agggtggatt catccaatgg attaaacagg aacagctagc 35460
tcgtaatatc tggtttactt tggcttctga tggtaatcaa aacagacctg gtattcgtcc 35520
tactgctagt aaacttgtta gatttaactc tattcttcca ttgtttaaag caggtaagtt 35580
ctatttccct gaggaagtac gtgaccaacc tgccatgttg gaacttatgg atgaattgag 35640
gttggctacc atagagggat ttaagagcaa gcatgacgac tttattgata ccattagtat 35700
gttgggttgt ctgaagactt ggaggccgtc acaggctgct gagatggcta aggatggtga 35760
tgaaatcttt agcttccctg aagctaccaa accaaatggt atggatagct acttagcgta 35820
aagcttctta taatgtggtg acgattaata accgggacta cattatgaaa ctctcagatt 35880
tatttacggc actgtcgtat ggggagcttc gtcagttata cattggtaat aacgggcaag 35940
gtattcgtcc agaggattac cctcaagtta tcttccaatt gaatgctggc ttagctctcc 36000
ttcattccag atttccatta cttgagaaac aagtgattgt tcagcaatta gaaaacattg 36060
ctacgtatta cttacgtaca gagtttcttg tttctaacaa acagtccaca cagaagtata 36120
aatacttgat tgatagtgtt gaccatccat tcacccaaga tatcattcga gtagaatcag 36180
tttatgatga gtgtggatgt cccttgtttt taaatgatga accacactgt aattcaatca 36240
tgtgtccgtc atttgattct attcaggtga ttgctccaat tgaaagtaat gcattgttcg 36300
ttacatatag agcaaatcat cctatcattc caactgatac tactgatttg aatattgaaa 36360
ttcgtattcc tgttagtcat caaaaagcat taacatactt catagcatca cagttttact 36420
ccaactcacc taatcaggaa acttctgcaa aaggtattga gtggactcag cgttatgaag 36480
ctgaatgcaa acgtattgaa gacttggatt tgaacaatca gtatattggt caaaccaata 36540
ctaaacctga aagaagagga tgggtgtaat ggctattaga acagtcaact cgtgttgtgg 36600
taacgatggt gctttagtag agcactttat cggtactgcg tatgatgtgg ttaaaaacgt 36660
ttacgataat ttagggattc ttcagtacat ctatgatttc ctgaaccagc atggtgtgtt 36720
ggttacggta gattcagtag atgaactgaa agccttaaat acagatatga agtatgctcg 36780
tgtatacact tacagcactg ctgctggtta tgggtataca gactatttat atgtagatga 36840
tgatacttct ggaatcattc caaatgatgc tactgctact ggtacttgga ttgaagtagc 36900
ttcatctaca tcattaaata atgggtctgc atatattcca tatatttata ataatggttc 36960
tgctattggt ggagaaacta ctattgcagt tcctaatgat actattggtg taccatatat 37020
tattaaaaac catggaactt atatagttac attgggctat acgtatgacc ctattacact 37080
cactgtaacc ttagatgatg ccttggctat tggggatgag gtcattctat tattatccgg 37140
tactcctgct gtacctactg accctactgt atctgattgg gttcagatta actggttata 37200
taatggcggg tatgctgtag gtggtgaaca ggttattgct gttccttata attttgcgtc 37260
tgtaccagcc atttataaga atggtttacg ttactatgct gggttagctg cacaatccta 37320
tatgattgat gtagaaaatc agcgtgtcct tttaactgaa cctcttgcac ctaatgacag 37380
attgattgtt caacttgggg gtgaagttca aaccttcatt atgactgacc gtacagttca 37440
ggaagtagct cgttctgcta atgtgcatga gaatgaagtt attctgagta ctaataccac 37500
tcagtatttg aatggtatga aaattatata tgatgtagtg gcacagaaga tttatggtct 37560
tcctactttg cctactaacg tgtatatcaa ctctgtatca aatggtcagt taacatattc 37620
accaggaaat attactgttg aattgttacc tatgcctgat gctgagttag atgcatttaa 37680
aaacacctta gctagttcta atgggtacac atatattggt gggttgagtg agaggtttag 37740
tttacctaac aagtgggtgg ttgtagataa tgccccttat tatggtaacc tggaattagc 37800
agttacatcc aatagctcta ataccgcatt cctgttaggc aaaggtaagt cttatgattt 37860
ccttactttc cgtactgccc gtaataccaa aaagaactta acgttctttg gttctggtgt 37920
accagaactg gcttctgata aacgtagctt tgttcctggt actggtacta ttatccgagg 37980
tccaatgaag actgaggtat ctggttggca attagttaac ctgggtattg actgtggtgc 38040
acagacttca gtagaccttt ttggtggaga gtgggatgac cctattcaat gttatggatt 38100
ggggaataat gccaatgtat ttattgataa tgttaaaaca ttaagccata ccaatctatc 38160
cggccctaac tacccaggca ctcacagttt actgcttgag ctactagatg gtgcttacgt 38220
tggtaagatt gaattaattg gtggtttcca tggccttacg ttgaaaggca ctaatattga 38280
cgtaatggat gctcactgct acgggcaacg tggcgatgcc tttatcatca agtcagattc 38340
cgcagcaact gctgctgata tttatatcgg caggataaaa gttggtttgc gtaacaattc 38400
tggatttact gatgtgacta tgggcggcat ctatgatgct catgatagcg taaatataga 38460
taacgtagag attggttctc tacaggtata taacgcttcg tggggattaa ttccttctga 38520
tgccaatact ggttatatct ctaatctgag aattaatagt tatgtagcaa tgaatgtcta 38580
cggtaattac tactcacttg ttattgatgg tcgttgtgtg ggtgtaaaca tcggtgagca 38640
tcagtgctct aatacatccg ggggtattag tatcgaccct aatgcagtta acgtaactct 38700
tggaaatggt ttctctaagg gttctactaa gtcagggtat gcattaggaa gcaattcact 38760
tgtacatggt aatctcattg ctgatgaaaa tggtgaattt ggggtggact acaaaggtgg 38820
tgttggtttt gatgcttcta agattacggg ttatagcaat acctttggtt tggtctctgc 38880
tgttcctacc gcattaaatg gcaatccacc tcaaaatggt tgggcagata ctggtgcatt 38940
tatggtacaa ctcactggca aaactgtgac tgtatccggc tcaatggttg taggtactgg 39000
tgccgttgca tataccacat tagatatttg ccgacctaag aaacgtatac caattgcagc 39060
ctggggcgta gcaggtagta ctatgattcc tgttgagtgt tacatcgaaa ccaatggtaa 39120
tcttaacgta gttggttacg catccattac agcaggtaat caaattcatt tctccggtca 39180
gtacctgatt tactaaatac taccccctct tcggaggggt tttacttaga ataaaaagag 39240
gtatgtatga aaagtatgtt cgctcaagat ggttctggtt ctgctggaat taagaccaat 39300
aaacaagtta ttgctcgtca cttcggtgta cagcaatctg aagtttgcta tgtaaaacct 39360
tcacagccat taacgggacc agtccatcag ttactaaata gttaagtgct agaattgtag 39420
tgtcaggttc taatagagac taaagcaaaa ggccccttat ggggcctttt tttctagata 39480
ctcacgaatc tcagtcatac ctcgttctaa ctctccatag taaaaattaa gtcttcctac 39540
tgatgaaggt gaacgtctga tatcaatcag aataaacctg actcgtggag atttgattcg 39600
ttccaacatt gcattgatac ctaaacgtaa tgctttattg gagttgtcat tgaacagagt 39660
attcagatga agtgtcagaa gagcatcagg atattcctgt actcctattt catcttgaat 39720
ctctggttta ggtccatatg gtttatgtct gcggaaatag gatttaacac ccataaataa 39780
cgctctctaa agggggtagg tttaacgatt aggatgtggg taaggtgaat atacctttaa 39840
gccgagaaag cccccagagg gggctgtatc actcaggatt aattgtttgc atcatacgaa 39900
cagaaataga tgtaagtgtg atggtttgtc cagagatgtt gttcatgcca agttgtatac 39960
ctgcaaccat gaatgggtca gtaggtccaa gggtataact ggctaagact gcatctctgt 40020
ttgtaatatc taatccttgt acttttgtac ttgggtcaga acctacaata gtgattccat 40080
ctgtacgtct agtttgagtt ctccattccc tagcagtacc cgcaccacca gatatcgttc 40140
ctgatatacg aacactaaac atgacctgtg tgtttttttg tctaacaggg aatagaagat 40200
taccccctac aatagataaa ttagcagtac cattaggttg tttggtaata cctggtagag 40260
caaagtagtt aaaccaagct ccatcagcaa ttacttgtga gccagaccat agaaataaat 40320
ccagtaaaaa cacttcagga aatggttctg gttcaggtat tacagggata gtaaatggaa 40380
gttcatttac attcttggca ccaataatat cttcaccaaa tgggctaact actaaaacac 40440
ccccactctg tttaggaaga tagtcattac catcctccag tatgtcatta atatcttcgt 40500
atgccatatg ccctcctata agtaggcaag gtcagtatac tctggatata aaaaaagctc 40560
ccgaaggagc tttgtaagta ttggtacttt tatggagcaa gagtaccagc agtacgcagt 40620
ttggctaaca gagcattaaa gtcagttact aaaccagcaa cagtagttgc agtcgagtct 40680
gcttgagccg ctgcttgttt aaccagacca gcagtagaag tagtagctgc ggacatagta 40740
ccatcaccta cacctgctgg tagttcagta gcatctttag cttgcagaac tttttcacca 40800
aaagtaccta ctaccagaat accaccatcc tgttttgcta cgatgtcttt atcatcagca 40860
agaatggcat ttacatcagc atgagttgta gccatggagc ctcctattta attggcaaga 40920
tgagtatata acatcttact ttgtagtcca tcctttggct ttaatgtatt cctgtagata 40980
gtctaattta ttttggtctg attggattcc gtctctgata tcgagaaggt cttgttgaac 41040
agttggagag agttcgattt cggctccatt gcccaagctg ctggcggctg ttcctgtatc 41100
tttggagagc ttacaggttg caagacctgc gttggcgaac tgcaacttac gagagccatt 41160
aacgatatca agcttaagct gcgcagtttc tttttcatgt ttagatttct cactagtata 41220
tttagcgtct aaagcatcta actcagcttg tactttatta tgtctttcta tttcagtctt 41280
catatctgaa gctgctttat ctgagatagc tttaagttgt ttattgtatt cagtggtttg 41340
gttagctagc ttagtctcat aactattact ggttatgtac caagcaaaaa aagctcccat 41400
agaaatgaga gctataacta ccaaaccaag tttaattaac ttggctttat ctatcatagt 41460
gatttgaccc ctctgcctat cttctctgca acatcccatg tattaccatt gaataaagca 41520
agacgtccta tagctctgcg atagatacct attttaggta caccattttg attacggaac 41580
tgagggagtt ttactttgag agtttcaata tcaccagcac gtacagcttg acctgtacca 41640
gtacctgctg caactgcttt aggacctacg ttaaagataa ggtcacagat tgcatcaaat 41700
tgtgcttggt tgaatttaag actagcaatg gaatcagcaa gtttaactcc ttcagccata 41760
tcttttttaa gaagtagata tgcttctgct tcagtcattg tatctgttgg tttgacatca 41820
gaaccatagt gtccaaaacc aatagtcaga tacttttcat ctttagtagc tcgataagct 41880
ttactggcaa atgtttccca agctgctgtg aacttgagtc cattgtctga aatgttacga 41940
ctcatcctat tttccccact actttagcta cattgccttg agccataaaa acactgacac 42000
agacaatcga gttaataatt acttcccaga agtgtacgtg tgagtattga tgcataagga 42060
tttgaatact aataattcct gtagcgacaa tgaggatgta agccaagata gaataactcc 42120
acttatgctg acatcctctt tgcctgaata gcatcaacct tatggcaatt gctgcgcata 42180
tacttgcgtt cagatagtct agattgaagt attctgtaat cattctgaac ctccagaagg 42240
gaagtcagga acctttgctt tattcattag gcttagcaat agcttgatag ctatggctga 42300
tgcaaccaat gctccaatag aagtatctac ctggatttct cttctaatga aaagagcagt 42360
tgtagcttct atgattttag tgaagtaatc agatgccatg atacccacta caaaagagat 42420
gaagaaaaat gtcagtcttt tgaagatagg tatatctgag tttgttagta cgtatacaac 42480
cgcaccagca aatgaaccga ttactacacc ggatggaaca cctgcccaat atgagagcag 42540
agtggttgca cttaacccag tcgtaatagc agttcccgat accggttcgt gcattttagc 42600
ctccagatat taaaacgtac ttctatgaag tagcctaaga atacctattt atcccaagaa 42660
ttattagaaa attcttactg agaagcttca taatgcatga agtatctata agctatatac 42720
tgctgacaaa tcaaacaaag ggtgtctgag atggctcaag atgaattaaa gtatgaaaat 42780
cctgaagaga taaaggattt aacagaatgg tccaatgaac cgactgttgc agacctacgc 42840
tctgatttgg aatcagctaa gtctgctcat agtggtcaga tgaccaagat tactcattgg 42900
ctcaaccaac tgaatgttac tggtgatgct aaacctaaga agacagatgg acgttctgct 42960
gtacaaccac gtcttattcg taagcaagct gaatggagat atcctggttt aacagaacca 43020
ttccttagta cagaagagtt gtttaaggca gagcctcgta gctgggaaga taagaaagca 43080
gctatccaaa accaaactct actgaattat caattcaata ttaaaatcaa taaggttcgc 43140
tttattgata actatgtacg tgctgctgtt aatgaaggca ctgttatttg tagagttgga 43200
tggattacta aatcacatat ggaaactcaa agagttcctg tgtactcgta ttatgcaatt 43260
cagattccag aacaagctca ggcattgcaa caagcaatgc agatgaaaac tgaagactac 43320
aataactacc tcaatctacc tgaagatatt caggcatctg ttgagtactc cattcagaat 43380
aacggtttct acatcgctcg tcagactggt gtagaagaag tagagatgga agttattgat 43440
gtgaaccaac ctactgttga agtagtgaat tcacagaacg taatcattga ccctaactgt 43500
gatggtgatt tagataaagc tcagtttgtt atttatagtt ttgagactaa taaatccgag 43560
ttagagaaag atggtcgata taccaatctg gataaaatta ccattgattc tgctaatcca 43620
ttaaatgttc ctgaccatat agctactgat aagagtggtt tccagtttaa ggatgaccct 43680
cgtaaagtct tggttgctta tgagtattgg ggatattggg atattgatgg aagtggtacg 43740
actaaaccaa tcgtagctac cttcgtaggt aatacgatga ttcgtcttga agagaatcca 43800
tatccagata agaaagtacc ttttgtcact gtaccttact tacctgttcc tggttcatta 43860
tatggggaac ctgatggtgc tctattagaa gagaaccaga gaatcattgg tgctactaca 43920
cgagcaatgg ttgatatctt ggctcgtagt gccaatggtc agacaggtat taagaaggga 43980
atgttagatg tcaccaacaa acgtaagttt gataaaggtg aagactatga gtttaacgat 44040
acggaccctc gtttaggtat cttcatgcat accatgcctg agattcctca gtctgctcct 44100
ctgatgattc agtatcagaa taacgatgca gaagctctta caggcgttaa atcattctca 44160
caaggcattg gctcacaagc tcttggtgat gtcgctgctg gtatccgtgg agcattagat 44220
gcagcatcta aacgtgaact aggtattatg cgtagactgg ctcaaggtgt tattgagatt 44280
ggtcgtaagt ttacttccat gaatgctgag ttcttgtctg aagaagaagt agtacgtatt 44340
accaatgaag agtttgtaac tgttcgtaga gatgatttga ttggtgactt tgatattaaa 44400
ttatctatct ctactgctga agctgataac cagaaagcac aggaattagc tttcatgctt 44460
cagactatgg gtaatacatt accatttgaa atgtctcaga tggttctttc agatattgct 44520
agactaagaa atatgcctga cttagctaag agaattgaag gctatcgacc tcagcctgac 44580
ccattagctc aacgtaaagc agagctagaa attgcacttt tagaagctca aattagagaa 44640
actgaatcta aagcaattga aaatcaggct aatgcagggt ataaacagac ccatgctgca 44700
aacctgcaag ctgatacaga ccttaaaaat ctggattatg ttgaacaaga gtctggtgtt 44760
aaacaagctc gtgatgttga gaagattgaa tctcagtcta gagcgcaggc tcgtactaaa 44820
attattgaag cattacttaa taatgcttca agtaataaaa cccagtaatc tcttaatgag 44880
gaaacaaaat gagtcaggta gaaactattc gtattgagat tgaacagaaa aaagaaatga 44940
ttgcgattgg tgaggctatt ggtcgcatcg ttcgtactcg tgactggaag aaattaattg 45000
agtctgagta cttagagaaa gagcctcaac gcttggtatc tttactggct catcctggta 45060
tgcaagatga agcatcccag aaagagattc ataaccagat ggttgcaatt gcctacttcc 45120
gtcagtttat cgctactcag cttcatatgg ctgaactggc taaacaggct ttaccggaag 45180
accaggaaac tgaagcacat ctgttagcta ctgaaatcca ggagtaataa attatgagtg 45240
acagcatcaa ccaatccgtt ctcggtatgt cagatgctga tttcctcaaa caaccgatgc 45300
cgacctttga gtcggcttct tcacaagaag agcaggttga ggaagttcag cagaccgaag 45360
aaacaaaaga cacttctact cagaatcagg agcaggaaac tactactgaa caatctgagg 45420
aaactactga acaaaccact caggaagata atactcagga acaaactact gagactcaga 45480
ctgagacaac ttcagaggaa acaaatacag aagagcaaac tacttctgat aatgttccag 45540
atgctgaagc tcaacttaag aaactattta ctccatttaa agctaatggt cgtgagttaa 45600
aagtagactc agttgatgag gctattacac tcatgcagca aggagcaaat tacaataaaa 45660
agatggcagc cttgaaacca tctttaaaaa ttcttaagat gttggaaaat aatggtctgt 45720
tagatgaagg caaacttact tatctaatcg atattgataa gaagaatcct gaagctatca 45780
ccaaattgat taaagagagt ggtattgacc ctctcgatgt aaatgtacag gaagaaccta 45840
agtacaagcc tggcaattat tcagtatctg atgctcaagt taatctagat tctgtgttgg 45900
atagtattga acattcacca acatatgaac ggacaatgac tgtgattact caggaatggg 45960
ataacgacag taaacgtgta cttgcaaatg acccaggtct tattccactt attaaccaac 46020
acatgtcgaa tgggattttt gacatgattg atggtgaaat aacgaaacaa agagccttag 46080
gtaatttgag tggtctttca gatttacaag cgtatgaagc tgtaggtaaa cagttggctc 46140
agaaaggtgc ttttgcttca ctgcaaaata acactcagca acagccagtt gaaactaagg 46200
cacatattcc tacgcaaact gatactgaaa gggctgctaa gcgtaaagct gcaagtccaa 46260
ccaaacagtc tcagacaacg aaggaagaaa ccaatcttaa tcctttagcg atgtctgatg 46320
aagagtttga aaaagcgttt aactctaaat tccgttaaga ggtataaatc atggtcatga 46380
agtataacgc tccgaatggt actccatcga gcattggtcc tcagattcgt ctggactatt 46440
tctataagaa agcactggta gatgctgctc gtgaaatgta ctttggtcag ttggctgatg 46500
taaccaacat gcctaagaac atgggtaaaa ccatcaagct gtatcattac attccactgc 46560
tggatgaccg taacgtcaat gaccaaggtc ttgatgctgc tggtgctact attgctaacg 46620
gtaacctgta tggttctagc cgtgatgcag gtactatccc aggtaaattc ccagtactga 46680
ctgagaatgg tggtcgtgtt aaccgcgttg gcttcactcg tattcaactg gaaggttcca 46740
ttaagaaatt tggtttcttc tatgagtgga ctcaagagtc tatggacttc gatactgatg 46800
atgaactgga ttctcatctg attaccgaag cggttaaagg tgctaacgaa atgaccgaag 46860
accagttgca gattgacctg ctgaatggtg ctggtgttat tcgttatcca ggtgctgcta 46920
cttccgattc agacctgact ggtgaaggta ctgctactgt agttacctac gacatgctgg 46980
ttaaactggg tatcaccctg aatgataacc tggctcctat gcagactaag ctgattgctg 47040
gttctctgat gacagatact cgtaccatcg ctggtgctcg tgttctgtac attggttctg 47100
aactggaact gatgctgcgt aagatggcag acccattcgg taatgcagca ttcattccag 47160
tacagcagta cgctaatgct ggtactctgc tgcgtggtga gattggttcc attgcatcct 47220
tccgtatcgt tgtagttcct aagatgatgc gttgggaagg tgctggtgct gctgttacca 47280
ctaacccagg ttactatgaa actgatggta actacgatgt gttcccaatg ctgtgtgtaa 47340
gctctggttc cttcaccact atcggtttcc agactgatgg taaaactgtg aagtttacta 47400
cttacaccaa gaaacctggt ctggagactg catcttatgc agacccatat ggtgagaaag 47460
ggttcacttc tattaaatgg tactacggtt ccttaatcct gcgtccagaa tggattggcc 47520
tgattaagac tgttgcatcc tattaatagc tgatagtcta aaagagtggg gaaacccact 47580
ctttttttta tctaaaaata cgatacactt agctcgacca tctctggttc caaccagtcc 47640
ataaaaggaa aactcatatg tctgacaatg ttgaactgaa tgaacttgat ttactgaaac 47700
agcaggctga taccttaggt atctcatacc atccatctat tggtgtagat aagcttcgtg 47760
agaaggttgc agagcgtatg cagaatgctt ctgagaatcg tactaccgta gatgctggtg 47820
ctgttgataa cagtcgtaaa gctgcctacg aagacgctat gaagcttgta cgtattcgtc 47880
ttacatgtat ggaccctgct aagaatgaat ggcctggtga aattatcact gtgtccaatg 47940
atgttgtagg caacattaaa aaatatatcc ctcttcgtga agagttctat gttgatggat 48000
atcatgttcc taacatcatc tataagttcc tgcgtgaccg taagtattca gcaattaaaa 48060
ctgttgaaac tcctaaaggt ccaaaggtaa ctgctatcca agccaatgct tatagtattg 48120
aagttctgcc tcaactgact gagaaagagc ttgagaaact ggcattgaaa caagctgctt 48180
ctaaagctgc tgattaagga atagagacat ggctgacgtc attatcacac caatcacgaa 48240
tgaagacctg actacaaaag tagttgatgg tactggtatc tttgatgagt taatgacggc 48300
agccaatgct catctggata gtcagtttaa gaatgaacgt attactggta ctcaatatgc 48360
tgaagtttac ttaggacagc ttcaggcagt attggctaat gcagttcaat tcctaattga 48420
aagagataaa acatatctca ataacttgct gattaatgct caaattgaat tagctaacaa 48480
acaagtagag ttagctgaga agcaattaga gcaagcagat aaacagcttg aactcttaga 48540
gaaacagatt gaactccagc aggctcaatc cgatttagcc agacagaaaa tcaaaactga 48600
gattgctcag attgctgata ctgttgatgg tgttgctgtt ggcggtgtta tcggtgctca 48660
ggttgctctg tataaacagc agagagaaag tttcctgaga gatgctgaac agaaatcatt 48720
gaagattctg gctgatacct ggattactcg taagaccatc gatgatggtg ttgaggttcc 48780
agtgaacttc gatacggatg ctctaaacgc attcacacag aaggtggcag atggaattgg 48840
ggtaacaatc taatcacaag gagagggagc gaaagctccc tttattactt atgggtttat 48900
ttagtcgtaa aaagattatt tctgtatctt ctgtcacagt gaatatggca ggagatgagc 48960
aaatcaatta cctgcgtaaa actattaaca ctgctattac aggtggtgca gatattgcta 49020
cgacattaaa ccaagcatat ctgacgggta tgggaatcca gattaaacaa gcctatagat 49080
atggcaggga ttactatgct cttggtttac cagatggttc tatcttcttt ggtacgccaa 49140
ataacgaaga aatcatcgct atccttaccg ctattgaagg taaggaagta gttcttacaa 49200
tggctgatta tggtgctcct gatttatcct attggattga ggagtatttg actaacactt 49260
atcattggga cactgaccat ggcggtattg gtaatcctcc ttctggcgtt ctaccagatg 49320
cttcaatcaa ctacactatc gataacaatg gtttggtcac tattacgatg actaacccag 49380
gtgatactgc acctactttt acggaggatg tgtcattctc agtagattat gatgcacagt 49440
attaccatgt tatctatcgt gttaagactc ctggcactcc tacagtaact actgttactc 49500
gtgatacaga accaggtgat gaagaaggta ctgttactac ttcaactaca gataataact 49560
ttggtgaatt taccatcaca gatacaactg tagttactac tattgatact gttaataaca 49620
aaaccactat tgagactaca gtggttgttt ctactttatc tggtaaaaaa tattggatgt 49680
atgctgctgg tacaggtaca tatcctgaac tggatagtat ccttactgaa gtagtccgtg 49740
attctgctta ctatccagtt gtacctctcc gagtaaataa caaagattta actgaccctg 49800
ccaatgtcag tcctgaccaa ttctctacaa gtaaaaaact aatgaggaag ttaaaactta 49860
acttcttaga tttaggtgaa cgtattaatg agaatccaga tgtaggagat attgaccatg 49920
cattctttgt aatgggtatt tcattgaact ctcagtatga atcctctatg gattatctac 49980
atgagttctt caaataccta gctcaagtat ctccttctga taaggaagcg tatgttactt 50040
ggtacaatga gaatgttgat gaagatggca atattaatga actgactcct aagcctcctg 50100
taaataagtt gtacttaaga cagaagcctt atgatatctc tattgcttac cagtactctg 50160
acatggttgt taagtctggt tctattggtc ctgttggtac tgttacccgt acatcaggtc 50220
ctgcaagtgc tatcaatgta gttaatcaat ttgacgtatc tactgaactt actgctgacg 50280
ttactgttat tactttcaga aaacagattt ctgagactca gtatgaggaa gtagaaacat 50340
gtggattgca gcatgtaaat aatgtctaca gaggccatag cgtagatatc tcaggagaga 50400
aaagtcttaa tgaccctgac actgaagggt tccttatacc tctctgtgtg aacgttacag 50460
atgctcagca actggtagcc agaacacaga tgacatttga ttgcttacat ttggttgtaa 50520
acagttatga agtaactaaa gccaaatggt atcagacagg tattttcaaa gtagtaacca 50580
ttgttgtagc tgtagttatt gctgtttatt caggtggtgc attagcagca ggtgtacaag 50640
cagcagcggc tgctgccgca gcagcaggta catcagtagc attagcagta gctatctata 50700
ttgttactca agtagcaatt ggtgttgcta tctcttacgg tgtatcaatc gcatcaagat 50760
acattaatgc taatatcctg ttaattgcag gcgtagcgtt aatggcatat ggactgtatt 50820
cgcaatacac tacatatgga agttcaagta ctgaaggttt accttacgcc catgaagcaa 50880
tgagcctttc tagtgctgta ttaaaaggta ctaatcaagg gttccaaaaa cagattcaag 50940
atgctgtagc tgagatgaga cgtaatgagg aaacatatga gagacaaatg aaagaagtta 51000
aggatgctat ggatttgtta ggtaatacaa atcctaatgt agatattgat gcattaatca 51060
atgctgcatt ctttaacctt tttgaatctc caaatgattt cttaaccaga acattaaata 51120
ctaatccagg tattgatagt attgcgatga ttagtggata tgtggattcc acattatcat 51180
taccaaatga cttaagtatg aggcgcgtat aatggcatat aacttagatt ttacaggtat 51240
gaatcctaat caggattttt ccttgtttgg aagtaatgca tataatccaa tgcaaagtag 51300
tgtgcttagt gcatctccta ctacatttgg tggtgttact tctccagcta ccgatggctc 51360
attaatgagt tcatttaact cttggttaca aggtgatact ggtcgtactt tatttggagg 51420
tactgaccct aatactggct ttcaatctgc tggtttagta gcacctacat tacaaggctt 51480
aggttccttg ttctctgcat ggactggtat gcaatcctta ggacttgcac aagaccagct 51540
acagttccag aaagatgctt ataacactaa tctgagtaat tctattcaga gttataacac 51600
tcaactggaa gaccgtatta gaggacgtac ctctaactat gcaggcaaag aagaagatgt 51660
tcagacctat ctggctaata acagactggg gaaataatcc atggctcaga ttacttggcg 51720
taatgttgat gctccagatt taactggtgt cgcaagactc cagcaatctg gcgctcaaag 51780
tttacgtgat gcattagctg gggcattggg aattattact cagaaccaag ctttgagtaa 51840
tcagaatttc aatgttgcca gagagcgtaa tacggctgat ttgcagaatc agttgttagg 51900
gtttactgac ccacaagctc ttcaagataa tgcagcacag ttctccccag atgctctgag 51960
acagcagttt ggtgctgctt atgaccaagg tgctattaac cagactctgg ctaccagacc 52020
tggtcagctt cgtgctgatt tatcaagtca aattcagtta gagaaccaac agaaagaaca 52080
agcttctcaa ccttatgaga atcagttcta ttctttattg gctagtaatc ctgcacaagc 52140
tgaacagttc ttaagagcta atgaggctaa ctttgcagac tctcgtcagt tatatggtga 52200
tttaactaac cgtcgtcagc aacttgaaca agctgcatta cagagagcac agttagctga 52260
atctcgtgct gcaagacaag aagctagagc agatagaatg gatgctcgta ctcaacgtca 52320
aaaccttaac aactatgcta aggaattgaa tgaatgggta ttaaagaatc cagataaacc 52380
tattggtact aaagctgctg aacttcagac taagtatgat gttaaccctg ttacaggtaa 52440
tcaggttact ggtgcagtac aacagaatat tcaggcatta ggtgctccta ctcctgaaca 52500
agctgtgaag attaactctg ctaaagctca gttacttact tcagttgacc agtttaaaca 52560
ggatgctact gctgaggtta atgctgcatt cgcaggtgct ggtattagta ctaacttaat 52620
tgatgcttct caggataaga agactactca agatgatgtt attgataaat ggggtaaacg 52680
attaggtgat actggtaatg ctgcatctta ctatgatgaa gctaaacgag tattaggtga 52740
cgtttcccct gctgttattg ataaagtctt acaagactct tatgacaata actatttctt 52800
tgacggtggt tcgatgaaca aagtgttctc aaccaaaagc tcagttaagg ctaatgctga 52860
aaaagttaag aaggcacttg gtgacacctc tactagacaa gcatttcagg atgcgttacg 52920
tacagtggat tctcaagcaa ctactttact taatcagggg aatgctccta tcgttaatta 52980
tgctaggcag cttcctgctt ttaacactgg tgctaatcct gtagctcctt cgttagctcc 53040
tatcattcca gactatgctg gaagcagacg agcattgcga gagcaattac aatctgtatt 53100
gggtgacccc aatatcaata gagaaacagt acgtaaataa caaaaatccc tcctagtgag 53160
ggatttcttt tagtacactc tctatcagtt aatacccctt ttgaggattc aaaatggcta 53220
ctggttttga aaaacagcag cagttaggaa atcttactgc acaaaaagaa caagaaattg 53280
ctcaagccac tcaacagaaa cgagttgaaa tgggtggtcc tgttccagga gttgagattg 53340
atttccctgt tggttcacct gagtatcaaa ggggtgagga agctattcgt ctggctaatc 53400
agcctgatgc ttctgtaatc aacaatttca ctattccaac tactcagcca agcactccag 53460
agatgagagc taatcaggct gctattgatg ctactaaacc tttctatcgt agaatgattg 53520
ctcctcaggt atctcctgag tttggtggca caggtccatt tgacccatcc catcctaact 53580
ttgagcaacg tggacaagta gctactactc ctcctgctga acagtatcaa gctgatttgc 53640
aggatatgcc tatcagtgca ttaagagcca aatatggtaa tgctgctgta gctgaccgta 53700
tgcgtttaaa tctggctaat gctaatagcc aagcattaga aggtcagcaa gtagacctga 53760
ctgatatcgc taactctgct caaatctggg caggtaatac tgctcgtttt ggtcttggtt 53820
tattaaacct tgctacacag aacgttcaag atactttggc agaagcagta ggtagaccag 53880
aagaagcagg tcaaccaacc ctgttagata gaactggttt ttcagatgct cttaaaggtt 53940
tcgttgaaga acaacgtaaa gactattctc ctggtactca gttagaacag tctcagattg 54000
ctgcatctaa agaacgattt gctcaaggtg gtgaagaacg tattcgtaaa gatatcgaat 54060
ctggttctaa tccatacatt gcagaagcgt atgaacaagg tcgtcagtta attaacactg 54120
ctgaacagta cggtggagct aataacgcct taacagcatt gattgctgaa tctgctcctg 54180
accttttatt aggtggtatt gttggcaagg tagctgcacg ttcagctatt aaagatttag 54240
cagaacgtca tggtgcagac tttgctgcta agttacttcg tactgaaggt ggtaaacgtc 54300
agcttgagaa tgctactgag aaagcatttg ttggttatgt tggattccag gaagctggtt 54360
ctaacatgca gcagactctg gatgaaattg accagatgac tcctgagcag ttagctgcta 54420
aatcaccaat gtataaagac cttattgctg aaggttatga accatcacag gctaagagtt 54480
tagtacgtac tcaagcagcc aatatcactg ctggtgtagc tggtacattt ggtgctttaa 54540
ctggtttaat tgctgcacca tttgaatcca aaatactgac tccgtcttta attaaaggcc 54600
gaggtctggt agacctgtct aatcgtgttt cttccaacat tgcgagagaa acaattgaag 54660
aaggcattca aggcgctact ggtcaatttg cttctaatct gggtatcaaa ttatctgctg 54720
atgaacagca gtcactcgct gaagacattg gctctaacat tgctgaatca gctattgctg 54780
gtgcgggtat gggtggtgtt ggcactgcta tttctgagac tcctaaagca gtcacagata 54840
catttggagc aggtgcggga gcagttaatc aagttagaga aagaagagca gctacacgag 54900
cacaagagtt tagagacact gtggctgccg caaatgaaac tgttcaacca acagatacct 54960
cagaatcaac aggacaagta gagcctgagt ctattcgtgc tcaggatgca gtaacttctt 55020
ctgaagctaa ggcagtattt gctcctattc cagaagaaca aattagttct cctgattcta 55080
ttatgcgtat tgccagagct atctctaatc gtcagatgga tgaatctact cgtcgtgagt 55140
tagctactgt aggtaataac gttatctctg cttatgagga tgcattacct cagattcagg 55200
ctcagatgga tgctgctcca gaagctgata aagctcagta tcaaactgca attgataata 55260
tcaatgcagt cttagagcac cctgatgtac aggctgtgcg tactactgct gaaagtttca 55320
agatgactcc tgaagaagtt aatgaagtat tctctaaatt accttctgag attactcctg 55380
agacttatca gtctcctgat gtacagtcag gtgttaaaaa tgtcttggct cagattgacc 55440
ttaactctca atccatctca ccagagcaag cagatactct gattaactct gctgatacta 55500
ttggtttgac tgataaccaa gttaatcaac tgagacttat ctctgcatct ggtaaagcta 55560
ccagtgatgt atccaatgat gtacgtaatg gttcagatgg gtttgttggt gtaaaccaat 55620
atactcaagc aatcatcaga gcaatggcta ctggttctac ccgtagagct aatgcaatgc 55680
tcgatatgat gaataacttt gctaaccaca tggatactaa agctgctgca ttccagaatg 55740
ctgctgctaa ctttactggt aaccagcctg tagaagtaat caatcctgct actggtcagc 55800
cttatatgtc tttggatgga actccaatga cgtatcatcc agttcgttct aagaacctca 55860
tcaatgagat tcagaatgat gctaatgcag ttcgtactgc tgctaatgct gctgcacagt 55920
taattgctgg tgaggctgtt actgtacctg aaacacaggc acagcctact gctacacctg 55980
ctactgagac tactcaggaa gctgctccag tagaagaaac tgtagaagct cctacaacga 56040
cttctgagac gacttctgct ccagaggtac aaacaccagt ccagattgaa acagaggctt 56100
ctacccctgt ttctgagcct gtacaaggtg aattgtttga taagccagaa gcagttcaat 56160
ctgatatcca gttaactgaa gaacctactg aacaagtaga gactgattta atggataaga 56220
accagatgga acttggttta gataaaggtg ttaaccacga tatcttagct ggtctgcaag 56280
cgactactga gcagtctacc gctgcctctt tatcagaagc tgataagcaa tatcagtcta 56340
ctaaccaagt taagaaatgg tttaaaccaa ctggtaaacg ttctgcattc ctgagaacga 56400
gtaacttctc tggtcgttta actgaaatct ttaacaatgg tcagaatcca gtaacccagt 56460
tacagaatct gttcaaagga tttgttaaac cagaagctgt tactcctaag gaagcacagg 56520
taatgagaca gtatgctgct cttgttcctg ctattgaaca ggctctgatt gagtcctggg 56580
gaaaactgac tcctgaaggt cagcgtatct tctgggaaac taacccggtt gaatactttg 56640
atgaggtacg caacatcaat ggtgagaatg tttacttctt acctcagcca gttattgaag 56700
caatgactgc tggtatgttg cagtggtttg tacgtaatgc gtctgaaact actttcaatg 56760
atgacagaac catcatggat attcttggtt tagataataa aactaaacca actgtagaac 56820
agactgacct gttaagaaat gcaggtactg accgtcagaa tgttatcgat gatttaagca 56880
gagaagtatt cggtatctta ggtatcaatg ctgacagaaa tactcctgtg agtattcgtg 56940
atgctgtgtc taaaggtttg gcaactgaag tactgaacgt aatgattaac tctaatctgg 57000
ttcaggaaac agtaattaag aactctgatt tagcttctgt tggctctacc aaagttaatt 57060
cttcaaacat gaagaactct cgtgtgttta tcagaatgaa tactgaatca gaagctgcta 57120
atcgcttagt agaactgatg cagaattcta atgacctgtt aggtcaactg actaacccac 57180
aaagagagaa gcaaggtgca ttcttaggta ctccacctaa aggccaatcc aacattgtta 57240
agaatggtgg tggtcaggtg attcctgaga agatgcttgc tgcacgtaat aaagcaagta 57300
accaacctca ttacatcaat atgggtctgc atgacctgat gatgaataaa cttggtactg 57360
aatggattgg aagaatgttg ggtatgcagt ctgaagagaa tgctaacgca gcacacttac 57420
aatcaattca gggttcaaac cgtactatcc agagagatat tcaaactctg gagaatggtt 57480
taactcgtgt tgctaatgct ggaatggatt tagctactac tccaatttac tttggttata 57540
acgtaatcag taacttccgt atgatgctta actctggtga tttcaatcct cagagcgcta 57600
agttacatcg tgagttagtg actgtagctc catctactat tgagttaaat aatccgcagc 57660
atggtgcttt cctcgattat gcaattgctc aaggcttaga tatttctgta gataagatgt 57720
catctgaatc tgccagagag aaactgagta aagctatcaa tactggttta ttcagagatg 57780
ctattaacat cctgaaagct gctgagaata ctgatgatat tagtgaagca gaccaggaaa 57840
ctctgctgaa tgcagtgaat gcaggtaaag agaagactaa aactcttcat gctctgtatg 57900
cacaagctca gtatgaactg gctgtagaga atggacaaac atcattccag acccatgtaa 57960
tgttggaact ggatggtgta accaatggtc cattcaactc cattgttcag cttggtttga 58020
aagatgttaa ccaagacctt ctggataaac tccagaaagg tggtttgttc tatggtgaat 58080
cagaccaact ctacaatgat gctgctgaac agcctgggtt ccttgacctg tataaaactg 58140
ctgctgctgg tactcaacag tacatcaaca atatgatgga ctctttccgt gctattccta 58200
atagaattgc agctatgaaa ggtaagtctc gtcaggaaca gcagaaggaa actagaaaca 58260
ttgaacgtct gagaaatcag atgcgtgtaa ctctggctgc ttccaaatta attggtgatg 58320
tgaacattgt tgaaggtgag caggttgaac acccaattca gattggtcgt ggattactta 58380
agaatccagt aactgtaacc gtctattctg gtggtgctaa tgccatcaac agaaagattg 58440
ctatgggtat tgttactggt tactacgaag caattacaga agctgtgaga gcagtagacg 58500
cagctacttc agaagatgaa agacaagctg ctattgctca tatcactgac ttaaccaata 58560
ccactaatga actgactact gctcaggtat accgtaatgg tgcttggcag cagattggct 58620
ctccaattga ccttggctct gatgtaacta agtttgagtt tacttctgct caaattgaag 58680
caattactca gaacatcaag attggtattg gtgctggtgt taaccagtct attgctaatg 58740
agtttggtac tgtaattaac cgtggacgta tgatggtcta tggtgcggct ttaatgcatg 58800
aagtattcat gtctcgttgg aacaaagctg tttcagaacg agaagcagaa cttcgtaaat 58860
caggtgaact ctctaagtat gagtctttat ctcaggctca atatgatgag attaaacagt 58920
cactgattac tcagatgcct atctttaact cttggttcac tgctaataat acagatgctg 58980
aatctctgaa taccggtatt ctgttaagtg aggaaggcag acagcctgta gactcttatc 59040
gtttagagtc cagaggtcgt agagagatgg acggtaagat gcgtaaatct acctttggtg 59100
tagatatgcc tacttacact gaaccaggta ctcgtgtaat gcctctgctt gtacagtctg 59160
tagaagccgc tatgcaggct attgctcgtg acattaaccc taacaatgct ctgaacgtat 59220
ttgatggtta catcaatgct gttgagcatt tagagtctgg ctctcaagcc attaaccaag 59280
gtgttctgca aagctggaat gagttcaact tacttcagga tttcaccaat cgttttgaac 59340
agggtatgga gaatgtagat attgccagtg aactggatgc tgatgtagct accagattac 59400
agcaatcatt cgattccatt gctgaagagc ttggctttga gcctggatta aatccagtat 59460
ctcctgaagt agtagaagca tttactaccc agcttaagaa agaagctgca cttcagactg 59520
ctatcaaaga agcattgttc aatggtgaag cagtaacctc tgttaaccag atgactggtg 59580
ctaacgttcc attcaatatt cagaatggta agcagactac tgaagaagct actgaccttc 59640
gttctcctct ggagcaactg aatgcatcag tagttcttcc tacactggat tcagtaacac 59700
tccctactgg tcgtactacg attccagagt ctgctcgtat gcgtgaagca ttagagcgta 59760
atggacgtgc tgtagcagga catgaaggtg ttcgtatcct gactaaagga gatgttgtag 59820
ctgctgttag agaagcttct aaagctcttc catctcgtca gaacagaatc actgattttg 59880
tactgaataa gattgaggct gctatcccat ctgatgtgaa tgtgtatgtg ggttcaccag 59940
aagctctctc aggcgttcag caagtaatga cacctgatag tcagatgcaa ttcaatgatg 60000
gcttcctggg gcttacagaa ggtcagaata tctatattgg tagccagtct attgaaactg 60060
taatgcatga attaatccat gctgctactg cacagactgt gatgtcctac tatgacaatc 60120
cagcaagtgt tacttctgct cagagagtag aagtagcatc tattgaagga ttaatgactc 60180
gctttgtatc tgagtacacc aatacgaatc cagaaactga ttcagtgatt cgactgattg 60240
ataaccatat tcaatctggt gatatggctc aggctactgc tgagttcatt gcatggggat 60300
tagttaaccc tcgcatgatt gatgctctga ctaccaaagg tattaaaggg gctattacca 60360
atgtgcttaa gtcactggct gactcaatta aaaagatgtt tgccattggt tcaaatccag 60420
agattgattc ttactggact cgtttacttg gtcatactgt tgccttggtt gatagcacta 60480
ctggtgctaa aacaactaat gagcaagggt tagctcagca gaatatgaac aatgtaaata 60540
atctggatgc agaaacacta ttcaataaat tggatggtgg caaagcatca gcagagcata 60600
tcaatcatct gactgatact ctggattatc tgcaatcgaa tattgttcgt ctgattaaac 60660
agactggaga agatatctct ggcttatcta attttgaaga taagttaatg ctggattcta 60720
ttgacccaga gattgataaa tctcctgatg cgttaattgc taatggcttt aacatgactg 60780
agaaagaagc attagtattc aaactgcttc aagtatctct caagtatggt ctggataact 60840
ttactccgag tgttctgact gcacgtagtc tgtatgcaca ggctaaacgt gaactgacag 60900
tacaggattt cctgactgac cctaataacc aatctcagtt tgagatggat ttagcgagac 60960
aacgttatga tgcggtgttt gggaattctg ctgttgtggc agatgctact ggtcgtagta 61020
atcgccttgc taactttatt gctttagtag aaaccaatga gtctctgcgt aataagctcg 61080
gtcagattcc tgttagagct accagagaaa ccccagctac taactttgta gaccgtgttg 61140
gtcaactggt taatagaatt ctggtttggt tatctggctt agctactcgt tcactgacta 61200
gtcgtgatat cgcatctcgt cttagtgtac tggctaaaaa cattagccat gtagatagca 61260
cttccagaga tactctgttt ggtcgtatct ggagaggttc tgagaatatc gttgagaaca 61320
ctcaagataa actcaaagca tctggtaata agctgcgtaa gcaactccag aaagtagaag 61380
ctaaagttcc tggttcagta gcatttggta tcaaagctgg ttcagcagta ttcaatgacc 61440
aagatgcaga agatgttcgt actgctatta ctaacatcgt tatgactatg aaccagaaag 61500
ataaactgaa tgctatttct gaactggtct ttgagtttgt tggtactaac gaaactaacc 61560
agactattca tactctgtta actcagaaga atatggctgc tgatagagca cgtcagatta 61620
ttcgtgagat ggttccaggt atgattgaga aatcatttgt atctctgaac caaacagaga 61680
aagaagctat tcagaaggta gttgctaaca ctgatatggc ttatctattt gctaataatt 61740
acagcctgtc tcgcctgaat gagttgttaa ctaatccagc agaattaaac aaagagattc 61800
aaagtatgga atctcagttg aattcctctg gtggtgttaa ctcagcattc tatatcaagt 61860
ctgcacatgg cttagcacag cagatggtat acggtgtatc acctctggct tatcagttat 61920
caaatgcagg agctattgct cgactgattg gtactggcag aaccagacct aatgatactg 61980
ctgtagaaac agctactcag ttgattgata ccttggcttc attatctgct ctgagagaac 62040
tctctgatgc tgataaagct actgtgtcta atgtgatgaa acgtgagatg actaaaggtg 62100
agccaaacaa taatggcatg acctttactc tgaaatacta tcgttcatta cagcaagctg 62160
aattagctaa agctggtggt gatgcaacca tgtcagcagt gaaaggattt actcctacac 62220
tggcagaccc taacaagaaa gttgtgattg catctgttaa agatgaatca atgctttctc 62280
gtaaagggta tgtacgtggt gcgaaagtac ctcgtgactc aatggacctt agacaagagt 62340
ctatgtacta ctaccattct aacaatggtg gtgaacctcg ctggattgct ggagtaatgt 62400
ctaccctgca aaccaccgta ggtggagtta acccactgac tggtagaagt gttaatgggt 62460
atatgactcc tggtgcatgg actgctaaag aacgtttagc tatgaatagc atgaaacgtg 62520
gagagattga taagctattt gacccagcta atccaatgcc gaagacgggt gcatcttata 62580
tgcaaccagt attaaaccaa gctggtacta tcatgggtta ctcttatcaa atgccaagag 62640
ctaacaaaga agctcatctg gatattgata atgatttcac taaagtgatg gctgcttggg 62700
aaggtaggat tgctgaagaa attcaggctg cacgttacaa caatatgctt atgcaacgtc 62760
ttcatgagat gtataagaaa gatgcgagaa tgggtaatgc ttaccagtat ctgaccatca 62820
ataagaattc aactgaccca caactcaaag aaatttatga gttgatgcca attgaaatga 62880
aggaaatggc taaagagatt tggcctaacg gtgatatcaa agtgcgtaaa gatttactga 62940
ataatgcctt tggttatcgt gaaccttctg ttcttaacat gtggacaggt cagagtggtt 63000
actctaaaga gtttgagcaa ggttttgtaa ccatggctga aacactcttt ggtaagaatg 63060
ctgctcgata tctgagactt ggtgaacgtg ggattatgga attcgtgaag gagatgaagg 63120
attggattgt ggtacgttca gtaattgtat ccgcagctaa ccttgtttct aacatggttc 63180
acttagctac tattggtatt agccctatca ctatggctaa ggatatgtct actgctgtac 63240
tggctgctga agaatacaga cgtaatgaaa aaatcattaa cgagtattct cactatctgg 63300
cagtggggca taacccacag aaggcaggtg agtatcagag acgtatctct gaacttaaag 63360
atttccaggc tcgtaatcca gtagctgaat taattcaggc tggtttgctt cctacaatcg 63420
cagaggattt aggtcagcaa gatgattact ctctgaaaga taaaatggta tctaaactct 63480
cacagtacac tgatggtgta ccagaaggag ttaagagagc agctaaggag attgcactgt 63540
ctagagatag tcatgtgtac ttcttcctta accgtagtat tcaatatggt gacttcgttg 63600
ctaagtattc aatgtataag caattgacta ctcgcaagaa taatcctttg gataaagagt 63660
ctgctctggc tcaggtaatg agtgagttca tcaactatga tgtactgcct agccgtactc 63720
gtgcttatct ggatgcaatg ggtttaactt ggttcctgaa ctacaaaatt cgtattcaga 63780
aaatcattct taaaaccatt agagagaatc caattagagc attgttcctc atgagtcatt 63840
taagctctga gtcttcattg cctaacatca cggatgctaa cctgattaca ggtagtttgg 63900
attacaacat cggtatggga acagcagcta acggattaac aattcatcca ttggttgcac 63960
tgttctaaaa gaaaagcccc aaggatgggg cttattttta aatcttacag ccaccccctt 64020
cacagtcatc taatgagaag tctttcagtt gctgttgcat ttgagcatca tcatatagtt 64080
tctgtttcag taagtagcct tctagcatcc agactttttc aatagcatta tctcgtgcaa 64140
cactacgacc aatagctgca ttgaaattct caggagatgc acaagcagat tcacctgtaa 64200
cagtaaatcc attttccaga accagtacac atatagttaa tagttttaat gaatctgggt 64260
aatcagtagc atcgcctggc tgtggattac caaatcgaga ttcaccttct actccagcag 64320
cagcactaaa gtaatgttca cttttaatct tactttgaat gtgtgcagca gtaatacgtg 64380
gagctttatc agcacctgct tcttgaatct gtttctctaa attatccaat ataattactc 64440
cgattcattc ttcttaaaga agaattcaat atagattatc aaaggccaga gtaatgaaaa 64500
tactccaatc cagaatatac cagcagggat tttgatagcg taatagaagc catcattacc 64560
aaatgctagt ctgattcgtt ctgttatttc cttggcttca tcattcttct ttcttccata 64620
aagaatataa agagttactg ctaatccaat taaaagccaa taaatcatac gattacccaa 64680
tcagtagcaa ataaatcacc ctgagatgga gtccaaccag gttgcatatt tccttgagta 64740
ttacgtaaat ccagatgagg ctgaatagtt actggaccag taatgcctgc ttcagcatat 64800
ttgcttccag gtcttacttc atccaaatga taaccaccag ccataatgac aaactgacct 64860
gctccgttcc atcctgaacg agcaattcta cgtccttctt ttactgcttc taaagcagca 64920
ctaaatgtca ttcccatctt ttgcgtcttc ctgttttaaa gccatgtaga tgaaccagaa 64980
taatcctgct atcacagcca ctgtcagaac taggggccat gcaaagaaga cccctactat 65040
gagagcagta atgaaagcag ctttaagtgc tttcatctag actcctgatt aagctgtacg 65100
agcaaacaga gatttagtct gttctttttg tcttgcgact acagcatcaa catctgcatc 65160
atcttcttct tcagctactt cttgagtagg ctgagtatcg atgatttgtt caccttctgc 65220
tggaagctcc gtaggagcct ctacttgagg ttttgtttct acctgttgta cttcttcaac 65280
ttcaccacgc agaatgccgt gagcaggctc tacgaagcca gaactacgtg gttgttgatt 65340
aggtgtcttt ggattgtggt cactggttag ttgctgagga acgatatcta gagttgctga 65400
ataaccattc tcaccacgac cagctttcag gtcgatatta atatcgttac ctggagctac 65460
attgataatg gtgaaagcat agttcttcag agcttccatg atttcgtctt gattcagtgt 65520
gatttgcatc aatctatttc ctctataaag atttctactc ttggctgttc tcggtctata 65580
gaccccatcc gaaaagccaa ctcaggaacg taaagataat tgtcatcagg taatttccct 65640
agctctacca aagcatcagc aaagaactta tcaactacag aacatacgtt acctacatca 65700
gttaagtgtt tagtggctgg atacattacg tatgtcagcc gtactttttt aaatgtaggt 65760
aaagcacgta tttgttcttc catctctagt ttgaaattaa ccttagcttt attaagtgtc 65820
atatgatgct cattgcgata tgcattgaga ttcagactaa ataatttagc tttggttttt 65880
tgtactctga gaggtgcgat aagtttgtgc atggttacaa tctcttgtca ttgaaagtga 65940
cagtatatcc ttgagatttg aaccattcga gttgagccat gatttgtgct ctcattaatg 66000
atttagaaca agcatgttcc caaccagtta atagaacatc aatttccttg tcatgtggat 66060
tttccaagat tccctggatg attttgtacg acactttctg gtaagctgta cctgagacgt 66120
tttggaacat tcttacttta attgccataa tccacctatt aaaaagccca ccgaagtggg 66180
cttctttttt aagagcgttt gaacaaggaa cttgtaccag tcttagcacc accagatggc 66240
agaccagtct tagcgccatc tttattgcct ttggctttat tgatgacttt acctgcccac 66300
ttacctaccc agtcatcctt gaacttggct tcggtaactc ccttttcgat ttctggtaca 66360
gtcagaccat catcgtaacg gaagaattta acgacctcgt taatttcacg agtctcgcca 66420
gtagggtaat atttaccatc atcgcctttt tcgtttttat ctactgtctg tttctggata 66480
cctgcctgaa tcaattgacc agtcagttct accagtacgt ctacttcagt aggacgttct 66540
tccttggctt caaagtcata cagtttgatg actttcttct caacatccag ttgagacagt 66600
tctttaccta cggtcagcag agccagactg ttagcagtca ggaagcttgg cagataatac 66660
tctttaccat ctttggtata ggtattctta ttgcctttgt cattccctga cagaacccag 66720
aactgctgac gtacttcttt accgtcaaca tcaccttgca ggttaattgc aactgcacca 66780
cttgctgcgg tagtgaagta tgcaaccttg accttcattg gatagacgtt ggtgtccagt 66840
acaccaccac cacccagaac gtccttttca ccttcaacgt ttgctttagt ttctaagttt 66900
tttaacagag acatttttta tccttactct tcgtagaatt catcgatacg tttaaacagt 66960
ttcatgacat cgttatcaat aaaactttca ttcgttttcc acataaaacg tggagaacgg 67020
atacgttcat gaacagtatc tttagtaata caggtttgga acacatgttt gaagccaagc 67080
atttcttctt cttctgttac tacaagaagt tcattctcat agtttttcag aagtttcaga 67140
ggtacacgtt tggcatatac gatacatgtg aagtacgctt caataccctg atttttcaat 67200
gcacctttaa caggaacttt ggtttcaaca accatctcgt tctcgttaag agttgctaag 67260
gtatgtgcag taaagattac tcgtttactg gatttagcta ccttatcttg catcaggttt 67320
ttgaagtact ggttgtaatt agcccaaccc tgcatggtat tagatgaacc aataacatac 67380
atagactcaa acatatccat caggaatgtc agtgagtcca ccacaatggt atggatatgg 67440
ggcatatttt cagcttcatc aaaagcttca tatacctgca ttgggtcagt aattacatat 67500
ctgtcgaact tattagggaa cggcagacgt ttgcctgatt cacagttcag atacattaca 67560
cctggatggt ctggcagatt catcaaagac gatgacttac cagatgatga agtacctgca 67620
atgagaacta ggtctttgat tggttcagac attatctctc ctgatagata aagaaagcct 67680
cattaagagg cttgttctga gagtttcttg ctgacacttc gcatgatagt gctatgaatt 67740
tcaatctctg gtaatgcatt gtccagcttc gaattgaagc ctaatacctt atccagaatc 67800
tgctgatact gaaggccagt gtcagctaac atcatagcaa attttagcag ttgattgttg 67860
cgatttcctg ctcccatacg ttgtgcgaac caacgttcaa gattgtcgag attccctaaa 67920
tcaaccatac gtttcttcag ttcatcattc ttactggttt tcggaatgaa tgctaaagca 67980
tccagcaatt ctccttcatt ggagaatgtt tcgcctgggt gggtagccca cttacggcat 68040
cgctggaatg tctggtcgtc aatcttgaat ggaagccatt cacagacatt attcatgaac 68100
tctctgaact caacttcatc taatttcaga tgatagttga ttggcagaat caatctgaat 68160
ctgtgattct catccgtgtg tcgcttagtg gtatgaataa agtacgtgta ttccttcatc 68220
agtactttaa cttcctcaat ggtagttcct tcatccacat caatcacaat gcagttaaag 68280
ccaggaatta cattggctcc atccctgtga ccatcaccac ttttaccttt cagtagagaa 68340
tggtttaccc agtgcatgtc ttccatttga cataactgct ggagattctc aaaagagatt 68400
ggttcactaa gatagttata agcaacatga ccactgtatg aaaccgtcag ttcatttaag 68460
tctgttggtt tgagtgactc acctttgaag aattcaattc catctacgaa tgtcttctta 68520
atgatgatgt gatgcttata gccataagct acagctaagt tcattagctc ttgcttctgt 68580
gcattactgc ctttgtagaa tggtaaatcc tctaccaagt ctacatgagt aacttccttg 68640
cctactccgg caatgtagtt tgccaactta acatagtttt tctcacggct aagtattcta 68700
tcgaatgctt caccagagtc ttcaaccagt ttaatagctg acattaattg ggcatcagtt 68760
acttcatcag tctcatcaac aaatgcgtag acacctgcaa gtttcagagc tttaaagtat 68820
ctgtgacgta gttctgcttt acgaatttct tcgtgttcag gaagcctgtc ggcaatctgt 68880
tcacaatgaa ctttgtaagc aatgagcttg atgcttacat ctctactgac gttaatcttc 68940
tgattatact taactgcatc agctaacatc ccaaagtgtt gagaaagctg ggcaatagta 69000
ttagacgatg ttgtatccgt taacatgtcg aagatttgtt ctggagtcat ttcctgttgg 69060
aaatcttctt tggtcgaata accaaacaag caacgacgag cataaccagc aacaagtaac 69120
gcatagaatt catcttcttc tttaccacca ttcagtaaac tgtttggagt accaaagaga 69180
agcatattgg taggtgtctt accttccagt tcttctcctc gttgagattc cgctgtgttc 69240
ttggttaact tttgcttgat taaaccttgg tcatacaact caaggaatgc agtcagtaag 69300
tcgaggttag agattaggtt cttacctaac tcatcacact cgaatgatgc agcaccagca 69360
ttagccaata acagtttata tcgaagttgc ttgagagcag gtacagtacc actatcaaat 69420
gagaatgcta atgcacctgt agaagcaaat tccttctcca gtttcactaa ctcatctgct 69480
gggtctgttc cgtttttact ggctctttgg gctgctaaat cgactaagtt ctgtccggct 69540
aagaatggga aagtatcatt caggaatgtt ctcttaaatt ggtttaagaa ttcaccttca 69600
atgatattgg ttgaatgtcc cttaccagac cctgatggag ccaagttcag aacatatacg 69660
ttaacaggca agttacctcg gtctaaggta tccagtgtga cacgcatact ggctgccatc 69720
ttagccataa agtaactaac ctgaacccta aagaataagc gattctgatt gagtgtcttt 69780
ttacagagca catcaaccat ctgttcgctt atggggtgat agtcagcact tgataagtca 69840
aacattgcat ctctctaatt agataatgag ttcaccagat gccaggtact tatccttttg 69900
tctgcatgag ttatacgcag cacagaactt acacgctaca acctgtccag gaactctgac 69960
aacgatgcct acacctccat cttctaccag acggagagta gcatcatgtt gattatcaaa 70020
gtttttggta ctacgtacca ttgagttagg gtttttgtag tatttgaaga caggttcaga 70080
gcgccatagt tcttcttccg tacactctgg caattctgat tcaggagcat ccttgtatct 70140
ttccaaagct gatagcttgg aggacacata ttgctccgtt tcactaacgg atttcaagag 70200
tattggatat tccaatatct tctgttgagg gtaatcctta ttcgctctag ccatatttgc 70260
tgaccaatct gtaaacaggt agtcaatgta catatggtca tggcgaatcc gttctgggtt 70320
aagccaacga tagatacttc cttgcatacg gaacttatcg tcattggctt tgttcatgta 70380
agtgaacgta ctggtagatt tgacgtccct gagtcgatac tcaagtacca tgtcaaactt 70440
tccaccaatc gtccaacctt tgaattcctt ttcaacacgt tgttcaagcc atacaggaat 70500
gatgtcatca ttcagttcat cgagtgatgg attgatacgc actgcttcaa tcactctctt 70560
aggataacca agagactcta atgactgttt gtaattactt ttccaggctc tctcaatcgc 70620
gtcatggaac gctgtaccca tacggctagg gatttggcta gctatatcaa tacgctgctc 70680
tccagcccca tttatacgtg ctgtcagtat caactgacga acacttttta ataggctggt 70740
agcagagata tagtttggtt tctcaacata atcgtaatca tcttgagcca accaaacagc 70800
catgcttaaa gctatgttgc ttg 70823
Claims (9)
1.一种沙门氏菌噬菌体，其特征在于，所述噬菌体为沙门氏菌噬菌体vB_SalP_TR2，其保藏编号为：GDMCC No:61403-B1。
2.如权利要求1所述的沙门氏菌噬菌体，其特征在于，所述噬菌体属于短尾噬菌体，呈多面体的头部和短尾结构，头部长61.5～62.5nm，宽59～61nm，尾部长34～36nm。
3.如权利要求1所述的沙门氏菌噬菌体，其特征在于，所述噬菌体vB_SalP_TR2的基因组序列如SEQ ID NO：1所示。
4.如权利要求1所述的沙门氏菌噬菌体，其特征在于，所述噬菌体vB_SalP_TR2不包含耐药或/和毒力基因。
5.如权利要求1所述的沙门氏菌噬菌体，其特征在于，所述噬菌体vB_SalP_TR2在pH值2～11、在4～80℃条件下具有耐性。
6.如权利要求1～5任一项所述的噬菌体vB_SalP_TR2在制备抑制或杀灭沙门氏菌制剂中的应用。
7.如权利要求6所述的应用，其特征在于，所述制剂为喷雾剂或洗剂。
8.如权利要求6所述的应用，其特征在于，所述应用为在食品杀菌中的应用。
9.一种含有噬菌体的组合物，其特征在于，所述组合物含有权利要求1所述的噬菌体vB_SalP_TR2。
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| CN108546685A (zh) * | 2018-04-20 | 2018-09-18 | 华中农业大学 | 一种肠炎沙门氏菌噬菌体lpse28及其在食品中的应用 |
| CN108588037A (zh) * | 2018-04-20 | 2018-09-28 | 华中农业大学 | 一种沙门氏菌噬菌体lpse34及其在食品中的应用 |
| CN112029732A (zh) * | 2020-09-05 | 2020-12-04 | 菲吉乐科(南京)生物科技有限公司 | 一种耐高温宽裂解谱的沙门氏菌噬菌体及其组合物 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108546685A (zh) * | 2018-04-20 | 2018-09-18 | 华中农业大学 | 一种肠炎沙门氏菌噬菌体lpse28及其在食品中的应用 |
| CN108588037A (zh) * | 2018-04-20 | 2018-09-28 | 华中农业大学 | 一种沙门氏菌噬菌体lpse34及其在食品中的应用 |
| CN112029732A (zh) * | 2020-09-05 | 2020-12-04 | 菲吉乐科(南京)生物科技有限公司 | 一种耐高温宽裂解谱的沙门氏菌噬菌体及其组合物 |
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