KR102396828B1

KR102396828B1 - 내열성 박테리오파지 ts3, ts6, ts13 및 이를 포함하는 살모넬라 억제용 조성물

Info

Publication number: KR102396828B1
Application number: KR1020200061814A
Authority: KR
Inventors: 유상열; 박해준; 김진우
Original assignee: 서울대학교산학협력단
Priority date: 2020-05-22
Filing date: 2020-05-22
Publication date: 2022-05-13
Also published as: KR20210145038A

Abstract

본 발명은 내열성 박테리오파지 TS3, TS6, TS13 및 이를 포함하는 살모넬라 억제용 조성물에 관한 것이다. 본 발명의 박테리오파지 TS3, TS6, TS13 및 이를 포함하는 조성물은 내열성 및 pH 안정성이 우수하면서 여러 살모넬라균에 대하여 뛰어난 항균활성을 발휘할 수 있다. 또한, 본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물은 단일 박테리오파지를 처리할 때와 비교하여 항균력이 훨씬 향상되며, 특히 액체 및 고체 제형의 식품 모델에 적용할 시에도 항균활성이 탁월하여 식품, 사료, 의약 제품에 다양하게 사용가능할 수 있다.

Description

내열성 박테리오파지 TS3, TS6, TS13 및 이를 포함하는 살모넬라 억제용 조성물{Thermostable bacteriophage TS3, TS6, TS13 and Salmonella controlling cocktail containing the same}

본 발명은 내열성 박테리오파지 TS3, TS6, TS13 및 이를 포함하는 살모넬라 억제용 조성물에 관한 것이다.

살모넬라(Samonella)는 그람 음성의 간균으로, 포자를 형성하지는 않으나 편모를 통해 운동성을 가지며, 감염 시 질환에 따라 급성패혈증을 유발하는 Typhoidal serotype과 만성 복통을 유발하는 Non-Typhoidal serotype으로 나뉜다. 특히, Non-Typhoidal serotype은 감염 시 심한 복통 및 설사를 유발하는 원인균으로, 국내에서 발생하는 식중독 사고의 주요 원인 중 하나이다. 또한, 사료에 오염되어 가금류, 돼지 등에서도 질병을 일으킬 수 있으며, 식육을 통해 사람에게 전염될 수 있기 때문에 중요한 오염인자로서 관리가 필요하다.

항생제는 페니실린이 도입된 1940년 이후로, 의료, 식품, 축산 등 다양한 산업군에서 이용되어 수많은 감염 질환에 대한 치료 및 예방을 가능케 했다. 그러나 불과 60여년 만에 항생제 내성균이 광범위하게 출현하여 각종 감염질환의 치료 실패와 치명적인 결과를 일으키고 있으며, 다제내성균까지 출현하여 미래의 심각한 위협이 되고 있다. 이를 해결하기 위해 WHO(World Health Organization)에서는 항생제를 대체할 수 있는 항균 물질의 개발을 촉구하고 있으며, 이를 통해 내성균 문제를 적극적으로 해결하고자 한다.

한편, 박테리오파지(Bacteriophage)는 박테리아를 숙주세포로 감염하고 사멸하는 바이러스를 말하며, 간단하게 파지(phage)라고도 한다. 일반적으로 박테리아보다 훨씬 작은 입자로서, 원형 혹은 선형의 유전물질과 이를 둘러싼 단백질 캡시드로 이루어져 있으며, 생물권에서 가장 넓게 퍼져 있는 존재로 추측되고 있다. 특히, 특정 균주만을 감염할 수 있어 병원균 외에 미생물 생태계에는 영향을 끼치지 않으며, 사람과 동물을 포함하는 진핵생물에는 작용하지 않아 인체에 무해하다고 볼 수 있다. 또한, 항생제 내성 유전자의 유무와 관계없이 작용할 수 있기 때문에 항생제 대체제로서 가능성이 높은 것으로 판단된다.

박테리오파지의 생활사는 크게 세균의 DNA에 삽입되는 용원성 생활사와 세균을 용해하는 용균성 생활사로 구분되며, 용원성 생활사의 유무에 따라 용원 파지(Temperate phage)와 용균 파지(Virulent phage)로 구분된다. 박테리오파지는 식품, 사료 등에서 첨가제로 사용되고 있으며, 임상 실험을 통해 의약 산업에까지 진출을 노리고 있다. 그러나 파지는 온도, pH, 염 농도 등 외부조건에 대한 저항성이 매우 다양하며, 개별 조건에 따라 그 용균 활성이 달라지므로 이를 보완하기 위한 기술이 필요하다. 특히 살모넬라를 제어하는 기술과 관련하여 식품 및 사료의 가공 공정 시 처리되는 열처리 공정을 고려한 내열성 파지의 적용 방식에 대해서는 개시된 바가 없어 이에 대한 연구가 필요한 실정이다.

대한민국 등록특허 제10-2003759호 (등록일자 : 2019.07.19.)는, 신규한 살모넬라균 특이 박테리오파지 SH9 및 이를 포함하는 항균 조성물에 관한 것으로, 살모넬라 하다(Salmonella Hadar)에 특이적인 사멸능을 갖는 신규 박테리오파지 SH9, 상기 박테리오 파지를 포함하는 살모넬라균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제에 대해 기재되어 있다.

본 발명은 다양한 살모넬라균에 대한 억제능을 가지면서 뛰어난 내열성, pH 안정성 및 항균효능을 가지는 박테리오파지 TS3, TS6, TS13 및 이를 포함하는 살모넬라 억제용 조성물을 제공하고자 한다.

본 발명은 살모넬라 균에 특이적인 사멸능을 갖는 서열번호 1로 표시되는 핵산서열로 이루어지는 것을 특징으로 하는 박테리오파지 TS3를 제공한다.

또한, 본 발명은 살모넬라 균에 특이적인 사멸능을 갖는 서열번호 2로 표시되는 핵산서열로 이루어지는 것을 특징으로 하는 박테리오파지 TS6를 제공한다.

또한, 본 발명은 살모넬라 균에 특이적인 사멸능을 갖는 서열번호 3으로 표시되는 핵산서열로 이루어지는 것을 특징으로 하는 박테리오파지 TS13을 제공한다.

또한, 본 발명은 상기 박테리오파지 TS3, TS6 및 TS13을 한 개 이상 포함하는 살모넬라 사멸용 조성물을 제공한다.

또한, 본 발명은 상기 박테리오파지 TS3, TS6 및 TS13을 한 개 이상 포함하는 식품 첨가제를 제공한다.

또한, 본 발명은 상기 박테리오파지 TS3, TS6 및 TS13을 한 개 이상 포함하는 식품을 제공한다.

또한, 본 발명은 상기 박테리오파지 TS3, TS6 및 TS13을 한 개 이상 포함하는 사료 첨가제를 제공한다.

또한, 본 발명은 상기 박테리오파지 TS3, TS6 및 TS13을 한 개 이상 포함하는 사료를 제공한다.

본 발명의 박테리오파지 TS3, TS6, TS13 및 이를 포함하는 조성물은 내열성 및 pH 안정성이 우수하면서 여러 살모넬라균에 대하여 뛰어난 항균활성을 발휘할 수 있다.

또한, 본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물은 단일 박테리오파지를 처리할 때와 비교하여 항균력이 훨씬 향상되며, 특히 액체 및 고체 제형의 식품 모델에 적용할 시에도 항균활성이 탁월하여 식품, 사료, 의약 제품에 다양하게 사용가능할 수 있다.

도 1은 본 발명의 박테리오파지 TS3, TS6 및 TS13의 배양결과를 확인한 사진이다.
도 2는 본 발명의 박테리오파지 TS3, TS6 및 TS13의 형태를 관찰한 현미경 사진이다.
도 3은 본 발명의 박테리오파지 TS3, TS6 및 TS13의 내열성 분석 결과 그래프이다.
도 4는 본 발명의 박테리오파지 TS3, TS6 및 TS13의 pH 안정성 분석 결과 그래프이다.
도 5는 본 발명의 박테리오파지 TS3, TS6 및 TS13의 단일 박테리오 파지(A) 및 조합물(B)에 대한 항균 효과를 분석한 결과 그래프이다.
도 6은 본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물과 비교처리군을 상온 조건(25℃) 및 저온 조건(4℃)에서 우유 내 항균 효과 분석 결과 그래프이다.
도 7은 본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물과 비교처리군을 상온 조건(25℃) 및 저온 조건(4℃)에서 닭가슴살 내 항균 효과 분석 결과 그래프이다.

본 발명은 살모넬라 균에 특이적인 사멸능을 갖는 서열번호 1로 표시되는 핵산서열로 이루어지는 것을 특징으로 하는 박테리오파지 TS3를 제공한다. 또한, 본 발명은 살모넬라 균에 특이적인 사멸능을 갖는 서열번호 2로 표시되는 핵산서열로 이루어지는 것을 특징으로 하는 박테리오파지 TS6를 제공한다. 또한, 본 발명은 살모넬라 균에 특이적인 사멸능을 갖는 서열번호 3으로 표시되는 핵산서열로 이루어지는 것을 특징으로 하는 박테리오파지 TS13을 제공한다.

본 발명의 박테리오파지 TS3과 TS13은 경기도 성남시 수질복원센터에서 수득한 하수 시료와 모란시장에서 구매한 오리 내장 시료로부터 분리하였으며, 박테리오파지 TS6은 서울특별시 관악구 봉천중앙시장에서 구매한 상추 시료로부터 분리하였다.

살모넬라(Samonella)는 그람 음성의 간균으로, 대부분 장내에서 증식하나 독소를 생성하지 않으며, 주로 오염된 음식 및 물의 섭취, 분변에서 구강으로의 경로로 감염된다. 제대로 익히지 않은 날고기 및 가금류, 달걀류, 채소류 등의 음식 식재료에 의해 주로 발생하게 된다. 살모넬라균이 감염되어 발생할 수 있는 대표적인 질환으로는 위장염, 패혈증, 장열, 그리고 불현성 감염증이 있다.

본 발명의 박테리오파지 TS3, TS6 및 TS13은 살모넬라균에 대한 숙주 감수성을 가져 이들을 사멸시킬 수 있으며, 상기 살모넬라균은 일 예로 살모넬라 타이피뮤리엄(Salmonella Typhimurium), 살모넬라 파라티푸스균(Salmonella Paratyphi), 살모넬라 엔테리티디스(Salmonella Enteritidis), 살모넬라 듀블린(Salmonella Dublin), 살모넬라 아리조나이(Salmonella Arizonae)일 수 있다.

또한, 본 발명은 상기 박테리오파지 TS3, TS6 및 TS13을 한 개 이상 포함하는 살모넬라 사멸용 조성물을 제공한다. 본 발명의 살모넬라 사멸용 조성물은 통상적인 살모넬라 제제 제조방법으로 제제화할 수 있으며, 제제화의 방법 및 수단에 대해서는 특별히 한정되지 않으나, 인체에 투여하는 경우는 약제학적으로 허용하는 범위 내에서, 가축 또는 어류에 투여하는 경우는 수의학에서 허용하는 범위 내에서, 제제화하고 그에 따라 투여경로를 정할 수 있다.

본 발명의 살모넬라 사멸용 조성물은 유효성분인 본 발명의 박테리오 파지 TS3, TS6 및 TS13 외에 필요에 따라 담체, 기타 보조제 등을 포함할 수 있는데, 상기 담체는 생리식염수, 증류수, 사료, 박테리아용 배지 등이 될 수 있으며, 보조제는 pH 교정 등의 목적으로 완충액 등을 첨가하여 사용할 수 있다.

본 발명의 살모넬라 사멸용 조성물은 액상, 반고체상, 분말상 등의 형태로 제조화될 수 있는데, 반드시 상기 3가지 제제에 한정되는 것은 아니고 다양한 형태의 제형으로 제제화될 수 있다.

본 발명의 살모넬라 사멸용 조성물은 경구, 정맥 내, 동맥 내, 복강 내, 근육 내, 경피, 비측 내, 흡입 또는 직장 등의 경로를 통해 통상적인 방식으로 투여할 수 있다. 본 발명의 살모넬라 사멸용 조성물의 투여량은 투여대상에서 살모넬라균을 사멸시키는 효과를 이루는데 요구되는 양을 의미한다. 따라서, 질환의 종류, 질환의 중증도, 제형의 조율 및 투여대상의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여 경로, 투여 시간 및 기간, 동시 사용되는 약물을 비롯한 다양한 인자에 따라 그 양이 조절될 수 있다.

본 발명의 박테리오파지 TS3, TS6 및 TS13 또는 이를 포함한 살모넬라 사멸용 조성물은 기존 항생제에 대한 내성 여부와 상관없이 살모넬라균을 사멸시킬 수 있으므로, 살모넬라균의 감염에 의한 질환 치료용으로 사용할 수 있고, 항생제 내성을 갖는 살모넬라균에 사용 시, 기존 항생제로 제거할 수 없었던 살모넬라 내성균을 사멸시킬 수 있으므로, 기존 항생제 대체물로 유용하게 활용될 수 있다. 특히, 식품업, 축산업 및 어업에서, 식품, 축산물 및 수산물의 항생제 잔류 문제 등을 해결할 수 있는 항생제 사용의 대안으로써 제공될 수 있다.

또한, 본 발명은 상기 박테리오파지 TS3, TS6 및 TS13을 한 개 이상 포함하는 식품 첨가제 또는 식품을 제공한다. 상기 식품 첨가제 또는 식품은 박테리오파지 H3, H6 및 H13을 한 개 이상 포함하기만 하면 그 제형에 특별히 국한되지 않으나, 대상 식품은 살모넬라균에 오염되기 쉬운 식품이면 더욱 바람직하다.

또한, 본 발명은 상기 박테리오파지 TS3, TS6 및 TS13을 한 개 이상 포함하는 사료 첨가제 또는 사료를 제공한다. 상기 사료 첨가제 또는 사료는 박테리오파지 H3, H6 및 H13을 한 개 이상 포함하기만 하면 그 제형에 특별히 국한되지 않으나, 살모넬라균에 감염되기 쉬운 가축을 대상으로 하는 사료 첨가제 또는 사료이면 더욱 바람직하다. 사료 첨가제의 일 예로 품질 저하를 방지하기 위하여 첨가하는 결착제, 유화제, 보존제 등이나, 효용 증대를 위하여 사료에 첨가하는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 완충제, 착색제, 추출제, 올리고당 등이 포함될 수 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있다.

한편, 하기 실험에 의하면, 본 발명의 세 개의 박테리오파지 TS3, TS6 및 TS13 모두 70℃까지 100 %에 가까운 PFU가 유지되는 것을 확인하여 내열성이 우수함을 확인하였고, pH 4 ~ 12까지는 활성이 유지되어 안정적이어서 중성 및 염기성을 띄는 조건에서 식품에는 적용 가능할 것으로 판단되었다. 또한, 박테리오파지 TS3, TS6 및 TS13을 조합하여 처리함으로써 숙주 감염범위를 확장함과 동시에 항균력도 증가시킬 수 있으며, 우유 모델 및 닭가슴살 모델에 적용하였을 때도 살모넬라균주를 완전히 저해하여 항균활성이 우수한 것을 확인할 수 있었다. 이를 통해 본 발명의 박테리오파지 TS3, TS6 및 TS13과 이를 포함하는 조성물은 뛰어난 내열성, pH 안정성 및 항균활성을 가져 가열 살균을 동반한 액체 및 고체 제형의 식품, 사료, 의약 제품에 사용가능성이 높을 것으로 판단된다.

이하, 본 발명의 구성을 하기 실시예 및 실험예를 통해 구체적으로 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예 및 실험예에만 한정되는 것은 아니고, 그와 등가의 기술적 사상의 변형까지를 포함한다.

[실시예 1: 본 발명의 박테리오파지 TS3, TS6 및 TS13의 분리 및 동정]

본 실시예에서는 신규한 박테리오파지 TS3, TS6 및 TS13을 분리 및 동정하고자 하였다.

경기도 성남시 수질복원센터에서 수득한 하수 시료와 모란시장에서 구매한 오리 내장 시료로부터 박테리오파지 TS3와 TS13을 분리하였으며, 서울특별시 관악구 봉천중앙시장에서 구매한 상추 시료로부터 박테리오파지 TS6을 분리하였다.

구체적으로, 병원성 살모넬라 타이피뮤리엄(Samonella Typhimurium LT2(c)) 진탕 배양액 50 ㎕, LB 배지 (트립톤(Tryptone) 10 g/L; 효모 추출물 5 g/L; NaCl 10 g/L) 5 mL에 상기 시료 여과액 45 mL을 혼합하고 37℃에서 6시간 동안 배양한 다음, 상기 배양액을 10,000 gDPTJ 15분간 원심분리하여 그 상등액을 0.22 ㎛ 필터로 여과하였다.

시료 배양액을 70℃의 항온수조에 30분간 침지시킨 후, LB 평판 배지의 위에 0.4 %(w/v) 한천 배지 5 mL, 살모넬라 타이피뮤리엄(Samonella Typhimurium LT2(c)) 진탕 배양액 50 ㎕, 시료 배양액 100 ㎕를 혼합한 후, 1.5 % 한천 고체 배지에 굳혀 37℃에서 하룻밤 동안 정치 배양하였다. 그 후, 플레이트 상에 투명한 단일 플라그(plaque)가 관찰되었으며(도 1), 이를 파이펫 팁을 이용하여 분리해낸 다음, 살모넬라 배양액에 넣어 37℃에서 배양하였을 때, 광학 밀도(optical density) 측정을 통해 배양액이 투명해지는 것을 확인할 수 있었다. 그 후, 10,000 g, 15분 조건에서 원심분리하고 그 상등액을 0.22 ㎛ 필터로 여과하여 박테리오파지만 남아있는 용액을 취하였다. 상기와 같은 방법을 통해 TS3, TS6 및 TS13의 파지를 분리하였다.

[실시예 2: 본 발명의 박테리오파지 TS3, TS6 및 TS13의 형태학적 분석 및 유전체 분석]

본 실시예에서는 상기에서 분리한 박테리오파지 TS3, TS6 및 TS13의 형태학적 분석 및 유전체 분석을 수행하였다.

1) 본 발명의 박테리오파지 TS3, TS6 및 TS13의 형태학적 분석

박테리오파지 TS3, TS6 및 TS13에 대해 에너지 여과 투과 전자현미경(Energy-Filtering Transmission Electron Microscope)을 이용하여 그 형태를 관찰하였다. 먼저 박테리오파지 희석액 10¹⁰ PFU/mL을 탄소 필름의 구리 그리드(Carbon films on Copper grid)에 올리고, 2 % 우라닐 아세테이트(uranyl acetate)로 음성 염색(negative-staining)한 후 30분 동안 건조하였다. 120 kV 전압의 에너지 투과 전자현미경(LIBRA 120; Carl Zeiss)을 이용하여 염색된 시료를 분석하였다. 그 결과, 도 2와 같이 TS6 및 TS13은 공통적으로 수축성이 없는 긴 꼬리 (long non-contractile tail)를 갖고 있는 것을 확인할 수 있었으나, tail-spkie 형태의 꼬리(tail)를 갖는 TS3 및 TS6과는 달리 TS13은 얇고 긴 형태의 tail fiber를 갖는 것으로 관찰되었다. 따라서 박테리오파지의 분류학적 분류(taxonomic classification)에 의거하여 복합형의 시포비리대(Siphoviridae)에 속하는 것으로 분류하였다.

2) 본 발명의 박테리오파지 TS3, TS6 및 TS13의 유전체 분석

본 발명의 박테리오파지 TS3, TS6 및 TS13에 대해 EDTA, 프로테나아제(Proteinase) K, 10 % SDS를 사용하여 파지 DNA를 노출시켰으며, 에탄올 침전법에 따라 DNA를 추출하였다. 이 후, 세니젠에서 Illumina Miseq (Illumina, 미국)을 이용하여 차세대염기서열분석(Next generation sequencing)을 수행한 다음, 해당 파지들의 서열 정보를 확보하였으며, GeneMark.hmm. software와 The RAST server software를 활용하여 오픈 리딩 프레임(Open reading frame, ORF)을 분석하였다. 박테리오파지 TS3, TS6의 유전체 분석 결과, 각각 41,568 bp, 41,515 bp의 크기를 가지며, 전체 유전체 서열은 서열번호 1, 2로 제시되어 있다.

[실험예 1: 본 발명의 박테리오파지 TS3, TS6 및 TS13의 내열성 분석]

본 실험예에서는 본 발명의 박테리오파지 TS3, TS6 및 TS13의 내열성 분석을 수행하기 위해 히트 블록(heat block)을 사용하여 60℃, 65℃, 70℃, 75℃에 노출시킨 뒤, 각 온도별로 남아있는 파지에 대해 플라크 형성 유닛(plaque forming unit, PFU)을 비교하였다.

보다 구체적으로, 3차 정제된 물 990 ㎕를 준비한 뒤 각 온도가 되도록 히트 블록(heat block)을 이용하여 조정하였다. 10⁷ PFU/mL이 되도록 박테리오파지를 주입한 뒤, 총 60분 동안 방치하였으며, 10분 단위로 생존한 파지 수를 확인하였다. 각 온도에 노출된 박테리오파지에 대해 씨리얼 딜루션 드로핑 메소드(Serial dilution dropping method)를 이용하여 PFU를 측정하였다. 0.4 % 한천 배지 5 ml에 12시간 배양한 살모넬라 타이피뮤리엄(Samonella Typhimurium LT2(c))을 섞어 15 % 한천 고체 배지에 부은 후, 20분 동안 상온에서 건조하였다. 박테리오파지를 희석배수별로 10 ㎕씩 분주하여 말린 후, 37℃에서 12시간 이상 정치배양하였다. 그 결과, 도 3과 같이 약 10⁷ PFU/mL의 박테리오파지를 각 온도에 노출시켰을 때, 세 파지 모두 70℃의 열을 1시간 동안 처리했을 때, PFU가 유지되는 것을 확인할 수 있었으며, 75℃에서 급격하게 감소되는 것이 확인되었다. 이를 통해 박테리오파지 TS3, TS6 및 TS13은 내열성이 우수한 박테리오파지임을 확인할 수 있었다.

[실험예 2: 본 발명의 박테리오파지 TS3, TS6 및 TS13의 pH 안정성 분석]

본 실험예에서는 본 발명의 박테리오파지 TS3, TS6 및 TS13의 pH 안정성을 분석하기 위해 다양한 pH 범위(pH 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)에서의 안정성을 평가하였다. 수산화나트륨과 염산을 이용하여 각 pH 범위의 LB 배지를 각각 제작하였으며, 각 pH 용액 990 ㎕와 10⁹ PFU/mL의 박테리오파지 10 ㎕를 희석한 후, 1시간 동안 상온(25℃)에서 방치하였다. 그 다음, 살모넬라 타이피뮤리엄(Samonella Typhimurium LT2(c))이 접종된 고체 배지에 희석배수별로 분주하고 37℃에서 12시간 배양하여 생성되는 단일 플라그를 계산하였다. 그 결과, 도 4와 같이 10⁷ PFU/mL의 박테리오파지를 처리하였을 때, pH 2,3에서는 활성을 보이지 않았지만 pH 4 ~ 12까지는 활성이 유지되어 안정적인 것을 확인하였다. 이를 통해 중성 및 염기성을 띄는 조건에서 식품에는 적용 가능할 것으로 판단된다.

[실험예 3: 본 발명의 박테리오파지 TS3, TS6 및 TS13의 숙주 감수성 분석]

본 실험예에서는 본 발명의 박테리오파지 TS3, TS6 및 TS13의 숙주 감수성 분석을 수행하기 위해 씨리얼 딜루션 드로핑 메소드(Serial dilution dropping method)를 사용하였다. 0.4%의 한천 배지 5 mL에 12시간 배양한 살모넬라를 각각 섞어 15 % 한천 고체 배지에 부은 후, 20분 동안 상온에서 건조하였다. 박테리오파지를 희석배수별로 10 ㎕씩 분주하여 말린 후, 37℃에서 12시간 이상 정치배양하였다.

파지에 대한 감수성을 보이는 숙주의 경우 플레이트 상에서 투명한 플라그를 형성하는데, 이는 숙주가 완전히 사멸된 결과이다. 한편, 약한 감수성을 보이는 박테리오파지의 경우, 드로핑 스팟(dropping spot)이 뿌옇게 되어 불투명한 자국을 형성하게 된다. 박테리오파지 TS3, TS6 및 TS13의 숙주 감수성을 분석한 결과는 하기 표 1과 같았다.

	Bacteriophage
Host	TS3	TS6	TS13	TS3+TS6	TS3 + TS13	TS6 + TS13	TS3 + TS6 + TS13
*Salmonella* Typhimurium
LTC(c)	C	C	C	C	C	C	C
SL1344	-	C	C	C	C	C	C
UK1	C	C	C	C	C	C	C
DT104	-	C	C	C	C	C	C
ATCC 14028	C	C	C	C	C	C	C
ATCC 19586	-	C	C	C	C	C	C
ATCC 43147	-	-	C	C	C	C	C
NCTC 12023	C	C	-	C	C	C	C
*Salmonella Paratyphi*
A IB 211	-	C	C	C	C	C	C
B IB 231	C	C	-	C	C	C	C
C IB 216	C	C	-	C	C	C	C
*Salmonella Dublin*
IB 2973	-	C	-	C	-	C	C
*Salmonella Enteritidis*
ATCC 13076	C	-	-	C	C	-	C
Isolate	C	-	-	C	C	-	C
Isolate	C	-	-	C	C	-	C
Isolate	C	-	-	C	C	-	C
Isolate	C	-	-	C	C	-	C
*Salmonella Arizonae*
KCCM 41035	-	-	C	-	C	C	C
KCCM 41575	-	-	C	-	C	C	C
KCCM 41651	-	-	C	-	C	C	C

C: 용균활성 있음

그 결과, 박테리오파지 TS3, TS6 및 TS13은 사람 및 동물에 대해 병원성을 보이는 다양한 살모넬라 아종에 대해 각기 다른 감수성을 보였다. 세 파지 모두 살모넬라 타이피뮤리엄(Salmonella Typhimurium), 살모넬라 파라티푸스균(Salmonella Paratyphi)에 대해 뚜렷한 항균활성을 보였고, TS3은 살모넬라 엔테리티디스(Salmonella Enteritidis), TS6은 살모넬라 듀블린(Salmonella Dublin), TS13은 살모넬라 아리조나이(Salmonella Arizonae)에 대해 추가적으로 사멸할 수 있는 것을 확인하였다. 특히, TS3, TS6 및 TS13을 모두 혼합한 조성물을 처리한 경우 실험한 모든 살모넬라 균주에 대해 항균활성을 나타내는 것을 확인하였다.

[실험예 4: 본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물 항균효과 분석]

본 실험예에서는 본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물 항균효과 분석을 수행하고, 이를 식품모델(액체, 고체)로 적용하여 확인하였다.

1) 본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물 항균효과 분석

본 발명의 박테리오파지 TS3, TS6 및 TS13을 조합하여 살모넬라 타이피뮤리엄(Samonella Typhimurium LT2(c))처리하였을 때, 그 항균력이 향상되는지 여부를 SpectraMax i3x (Molecular Devices, 미국) Plate reader를 이용하여 측정하였다. 37℃ weak shaking 조건에서 10⁸ CFU/mL (OD_600nm= 0.15)에 대해 MOI(Multicity of infection=CFU/PFU) 1의 박테리오파지 조성물을 처리하였을 때, OD_600nm 값이 감소되는 경향을 관찰하였다(도 5).

구체적으로 도 5의 (A)와 같이 단일 박테리오파지를 처리할 시, TS3과 TS13은 4시간 째, 저항성 균주가 출현하였으며, TS6은 항균력이 비교적 약할뿐더러 6시간 째, 저항성 균주가 출현하는 것을 관찰할 수 있었다. 반면, 도 5의 (B)와 같이 TS3과 TS6을 함께 처리하였을 때는 효과가 없는 것으로 나타났지만, 두 파지를 TS13과 함께 처리하였을 때는 항균력이 증가함과 동시에 파지 저항성 균주의 출현이 8시간까지 증가하는 것으로 나타났다. 이를 통해 박테리오파지 TS3, TS6 및 TS13을 조합하여 처리함으로써 숙주 감염범위를 확장함과 동시에 항균력도 증가시킬 수 있음을 확인할 수 있었다.

2) 본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물의 액체 식품 모델 적용

본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물의 항균효과를 우유 모델에 적용하여 분석하였다. 멸균 우유에 박테리오파지 10⁷ PFU/mL을 처리하고, 항온수조를 이용하여 30분간 63℃의 온도를 처리하였다. 그 후, 10⁴ CFU/mL의 살모넬라 타이피뮤리엄(Samonella Typhimurium LT2(c))을 처리하여 상온 조건(25℃)과 저온 조건(4℃)에서 48시간 배양하여 시간에 따른 균의 감소를 정량적으로 측정하였다. 이를 비교 분석하기 위해, 파지 비처리군(LT2(c))과 비내열성 파지 NH4 처리군을 함께 실험하여 본 발명의 내열성 박테리오파지 TS3, TS6 및 TS13의 조합물 활성을 확인하였다.

그 결과, 도 6과 같이 본 발명의 내열성 박테리오파지 TS3, TS6 및 TS13 조합물 처리군(Cocktail로 기재)은 상온 조건(25℃) 배양조건에서 12시간 째 살모넬라균주가 검출되지 않았으며 24시간에서 6.1 logCFU를 감소시키는 것으로 측정되었고, 저온 조건(4℃) 배양조건에서 살모넬라균주가 완전히 저해되어 검출되지 않았다. 반면 비내열성 파지 NH4 처리군은 살모넬라균주를 전혀 억제하지 못하는 것으로 나타났다. 이를 통해 본 발명의 내열성 박테리오파지 TS3, TS6 및 TS13 조합물은 우유에 적용되어 항균제로 작용할 수 있으며, 더 나아가 제조공정에서 가열 살균을 동반한 액체 제형의 식품, 사료, 의약 제품에도 사용될 수 있을 것으로 판단된다.

3) 본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물의 고체 식품 모델 적용

본 발명의 박테리오파지 TS3, TS6 및 TS13의 조합물의 항균효과를 닭가슴살 모델에 적용하여 분석하였다. 항온수조를 이용하여 각각의 10⁷ PFU/mL, 10⁸ PFU/mL 박테리오파지 조합물이 담긴 물을 66℃로 조정한 후, 닭가슴살 큐브(cube)(1cm x 1cm x 1cm)을 1분 30초간 침지 상태로 방치하였다. 그 후, 10⁵ CFU/mL의 살모넬라 타이피뮤리엄(Samonella Typhimurium LT2(c))을 오염시킨 후, 상온 조건(25℃)과 저온 조건(4℃)에서 48시간 배양하여 시간에 따른 균의 감소를 정량적으로 측정하였다. 이를 비교 분석하기 위해, 파지 비처리군(PC)과 비내열성 파지 NH7 처리군을 함께 실험하여 본 발명의 내열성 박테리오파지 TS3, TS6 및 TS13의 조합물 활성을 확인하였다.

그 결과, 도 7과 같이 본 발명의 내열성 박테리오파지 TS3, TS6 및 TS13 조합물 처리군(Cocktail로 기재)은 파지 비처리군에 비해 살모넬라 균주를 감소시키는 것을 확인할 수 있었고, 특히 10⁸ PFU/mL을 처리하여 48시간 동안 배양하였을 때, 상온 조건(25℃) 배양조건에서는 5.5 logCFU를 감소시키는 것으로 측정되었고, 저온 조건(4℃) 배양조건에서 3.5 logCFU를 감소시키는 것으로 확인되었다. 반면 비내열성 파지 NH7 처리군은 열에 의해 파괴됨에 따라 살모넬라균주를 전혀 억제하지 못하는 것으로 나타났다. 이를 통해 본 발명의 내열성 박테리오파지 TS3, TS6 및 TS13 조합물은 닭가슴살에 적용되어 항균제로 작용할 수 있으며, 더 나아가 제조공정에서 가열 살균을 동반한 고체 제형의 식품, 사료, 의약 제품에도 사용될 수 있을 것으로 판단된다.
기탁기관명 : 농업생명공학연구원
수탁번호 : KACC97036P
수탁일자 : 2020.02.04.

<110> Seoul National University R&DB Foundation <120> Thermostable bacteriophage TS3, TS6, TS13 and Salmonella controlling cocktail containing the same <130> YP-20-025 <160> 2 <170> KoPatentIn 3.0 <210> 1 <211> 41568 <212> DNA <213> Unknown <220> <223> Bacteriophage TS3 <400> 1 atgcagcaat ccgaattagg cgcccgcata gagcaccgcc gcaaagaaat cggcatgggt 60 cagactgaac ttgccttcaa agctggtgta tctcaaagcc tgattaccca cctggcaacc 120 ggtcgggtat ctaaggtgga ctgttttaaa atcttccaca tcgccgatgc tctcggcgtc 180 gaccctcggt ggttaagttt tggtgataca ggggcctgat ggcccctttc ttttattcta 240 aatccccatc aatcgccaaa acgtaatgtt tgatgtttgc gtacataaca taaccccaaa 300 accaatagcc aacaggccag gtaacgcaca ccactgtcag tatcaccaat tgcaaaaacc 360 acgcggcaat ctccagcaaa gtcatatgca gtacttttcg aattttagtg atacaaccaa 420 ataattcctt taaccccgcg cccaatcggt tactactcgc actgacgcag tccaggtaac 480 atggccggta tccggccttt acggatcccc acgggtccgc tatgaaattg tttatattca 540 ctctaaatcc ccttctgttg tctttatgtt gtcgttaggt tcataccggt tcttcggtct 600 cttacggtca tctatgcccg tcggtaacct gtaagtattc gttacaactt caccgttttc 660 gtcacaacca agcatcaatt cgccttcacg tagcatcttc tcaagcacca ggttcgttat 720 gctgtgatcg cctgctctcg cgactatttg ccgttgtgta aatcctcgcc cggtatcatc 780 ggcttgttgt aaatcctcaa gagcagacat aaccgattcg cgggcggaag catctttaga 840 ccgtttaaca gtacccttta cggaatcttt acctttacca tcgagtcctt cgttgcgttc 900 tttttcttca tccgtttcga acggctggaa gccccacggc atgagtacaa gagctttatg 960 tggttccggc aggtcgaggt taacgatttc accgtatccc tcacctccaa cgaattctac 1020 agcctggaac tctttcggcg gtggagcttc acgaaactgt accggttcaa gcaccatacc 1080 tatcgttttc tgctgcatgc cgtttttgtt cttggtgtgc gcaacgttta tttgtttctc 1140 ggtcgctcgg acaagcgtaa gttcgacgtc gacaccagca tacagcgccc cacttccgcg 1200 cgccttactc ccacctttcg gggtgtggtg gacaacgcct acagcgcctt tagtaccgtc 1260 gcgcacttct ttaagcatag ccacgacacg gcccatcccg tcttgcccgg ctgagttctc 1320 attgaacttg tctatccagt tcccgaaggt ctggttaaga gtgtcgaaag cgaccatccc 1380 gacaggttca ctgcccgcta tctggcgcat tttacgcacc agtttcttcg tatccgcgaa 1440 ttcaccagcg tcaagcacat gcatataccg cataccttca tcgccgtatt tagcggccaa 1500 tgcagcgata cgggtgtgcg tgaactcccc accctcacca tcaatataga aatgatgggc 1560 tttacgggtg tctgcccctg cgaaccgata cccggcagca ctgatataca tcatgccgag 1620 tgtgtagaac gatttgtacg tcccggattc accgacgata tcccagatgc agttagacgg 1680 catgtaccct tcgacgatga agtcagcctg tggtgccggt ggttcttcgt catccaggtc 1740 ttcctcatcg caagtaacgc cgggcgccca gccaagcgcc gcctccacac ggtcaaaagg 1800 taagccggtg gccgcacaag cgtaagccca catatcccgt ccgccaggat tcatcccgcc 1860 cgtagctacc aggtcggtat cgtggtacat ggtaacgttg ggtcgctcga agccgtcgcg 1920 cggccaacag aacaggaagt catcctgctt aggttcaccg gtagtgtatt gggtggcgtg 1980 ctccggcgtg gcgggcattt gcaggccgcg gtcggtcata cggccgccga actcgaaggc 2040 gaactcttca aacaggtcag ttaattcgga ttgctcaccc tcgggcactt tgtaatcgga 2100 agcgcctgta acgttaattt caggaactcc ctccataagc ttactggccg taatcatgcg 2160 gctactttcg gacacgatga cctgactccc gacaggcggg cggtacatcg gctgggacag 2220 ggtgaacccg gcgctatcta cgtcacgtcc tttaaggaag tgggtcaaca ggccgtaccg 2280 gatgcggata acgtcgccac cagtgaccgg tgtacgcacg ggcataacga cgcgatagcg 2340 cggcgcttcc gcagtgtgcg atgctgtggt gtagagcatc atagcgaaac gtgactcgcg 2400 caccatttca cagtcggtgg caaactcttc cggagtcgca ctatccacat cggcgtaagc 2460 cagggatgaa gacgtaacag atgcattgca gcggtagaac ataccttcgg cggcttcttt 2520 accggtagag cttaccgtgg cggtacaagc ggcggtaatg taacccgggt ccgtcttggg 2580 attccggcgc gaacgtttaa gcggctgcat cagttcgaca aactcatccc aggtgccgga 2640 agttgtggtg taaacgttaa tgtcagcgcg ttcctcacgg cgattgcgac gcgaccatga 2700 gtatgctaaa ttgcctgttg acatgtgctt ttcctttgta gtgtttaagg ccctgacgtt 2760 cgcgcgttgg ggcttttctt ttattcaagg tcttctgggg tcgcctggga gcctttaact 2820 tcagctactg ccgaatattt tccggcctta cttttaactg ctccgtctct aaccagaacc 2880 ttcaacgtgt tctcgatgag agaggaacta tagtactgaa aataagtgcg gcgcaagtca 2940 agaacactac acgacccttt tttacacgta agcgcgacta ccgttctgaa tacccttttc 3000 tggaaatcgg tcactttaca ttctccctaa tccacgcttc cactttctcc cggtcaaacg 3060 tacctggcat cctgcgaccc ataatccgga cacaacaatc cgggaacttg ccgtctctta 3120 accagttatt cagcgtgcgg cgggtaaccc caataagctc agctacttca ttctgtgtca 3180 ttctcaaatc ctccatctca gttagtaaag caagtatacc cacggcataa tgggaaatca 3240 actataaaca cacctattga cattttcatt tttttcgtgt aagttgtctc ttgtaacttc 3300 aagttactta agtttctgtt tcactttgtg agagcttagc ggttccgccc taagcggaaa 3360 cgcgttaagg cttactgaaa cccgcaaact taaaactcag taacttccct tccttagtat 3420 ctgaactgcc ctgggcgagc tatattctca tcggcagcat atctcttaag tatctgaatt 3480 ccttgatgtg ccgtagattc gcaaccgtaa aatgtaacga ttagcgggca ggttttagac 3540 ctgcccttta gttaccagat aataggaata aaggcacaac actggacaac ggcaaacgta 3600 actgttctct tgtatgccga aagattctta gtctactact catgaattaa cgcacttgcc 3660 ttgcggcaag cgcttacctg acgagggaca agaataaagg cactctaatg ggggcgggga 3720 cctccctgta aataccccca cggcaaaaga ttctaaagag tccgcgccgg tgattctcgg 3780 gtcttaaagg aggttgccgt ttatggctag ggttttagtg gtccccgggg ccgcatccgg 3840 acgccgatga tgatgggtgg atagttcctg gggtatcgtg gtggcggggc cgcacctggg 3900 cgctgttacc ggaaccgcag gaagcttgta acagtagttt aggattttac tgagtgactt 3960 acgctgctga gtagcagcgt accatttttg ttaatatttt tctatttcgc caaacgcaac 4020 aatcgaagcg actattgcag gaggtgcaac aatgaaaccg aatgaccttg taacctggac 4080 cgggcgtaac ggagagacca gacacagcaa agtaatatcg ctccacggca tctacgcccg 4140 tgttgagtgg tggcgcgctc attcgaagaa gccccgctac attacggtgc gacaggataa 4200 attaatcgta aaatagtatt gacgtaacaa aacataatag ggtactatta ctttatcgaa 4260 acgagataga ggagtgtggg ggtgaataac aaagacgagg tattagagta cctgattgac 4320 cagctacggc agcgggtaaa cagcaaccag tgtggagacc tgatgcagga ggtgcagtca 4380 cttaagaatc agttacgtga tgtgtcggtg ctggtggctg agttacagga agacaacatt 4440 aagaaatctg agcacatatc acacctgaca gggcaagtta aggagttgcg tatggcgccg 4500 gtaaaagcta cgggcgacgt ggaggcgtac aaacttgcgc gcaaaggtga acacgggccg 4560 gagcaatctg gtttccgtaa agaagatgct caggggtgcg agcacgattg gtacttttac 4620 gatacaagtg cgtcgatgat gtgcagaata tgcgggttaa agaaatgacc agtatcttat 4680 ttatctgggt actgtccgca ggccagatgc aactcgcggc atcggaaacg ttttacacat 4740 tagaggcgtg ccggtcagcg gcacgcgccg ccgagaatgc gcacttcctg tttcagggtg 4800 acaagccaaa tgattcagaa gtacgcgcta tctgttcgcc taagcgactc ggtaaacagg 4860 agaagtgatt atgagtctcg caaccgatat actgaaacac gccggcatca acctggcacc 4920 tccttcatcg gcggtaacaa agaaggttac tcgcgttaaa gaaaaggtta agcgtaaacc 4980 taaaccgcgc gcaaagccag ttaatgagat gccggacgtg tacccgcgca tccctggggt 5040 gcaccagcct aagtattgtg taggcaaagg tctgtggcgt gcgcactctt acgacgggaa 5100 gaaagtggta aaccttggcg agttcagtag ccaggcgcgg gcgcatatgg cggttaaact 5160 gtacaaactg tggcgtaaac gcgggcactc agatatcccg cacaagccat ccatcagact 5220 ttatacgttc agataaatcc gaattatctg ttactaaaat tgtcgtataa atgttaatct 5280 ccgaagggta aaccaacttt cggagatttt ttattgtgga cgatagatac ctttggctta 5340 gtgtggcggg cctcgccggg ggtgccgtat ctcagattaa gaagcgtgag gctatttcgc 5400 catggttgcg attgtgccac ctggttgcct ccgcctgttg tgcggtgtac gcatccccca 5460 tcattataag ttactatgaa ttatcacagt ctgagggtca gtacctggtt cctttcgggg 5520 taggtatgtt ctggcttaaa ttatttgagg ccgccgactt gtccctcagc aactttaagt 5580 taccgtgggg gaaataacat gctcaactca caaatcggcg ccatttgtct ggtcgcaatc 5640 atcgccacct cgttaattaa tatctacgcc gactgggttg aagacggtct tttcggccgc 5700 ctgctgtata tggcgtccgt tgttaccgcc gccggtgggc tagctcatct ctttaccggt 5760 ggcatcccgc cattcataat tacgactctc gtcgccgtat tcgcgcttaa atcggtgcgt 5820 cgtatttgcg tgaaaggcgc tcgctactac aaataccgga ggatgtatgt caaaccgaaa 5880 cattagtaac aacggcatca aattcaccgc ggcattcgag ggtttccgtg gaaccgcgta 5940 cagggcgacg aagaacgaga agtacctgac aattggctac ggccactacg gcgcagatgt 6000 gaaagagggc cagaaaatta ccgaaggcca gggtcttttg ctactgaaca gggacatggc 6060 taaggctgta gccgcggtag acgccgttgc gcatccgtcg ctcaatcagt cacagttcga 6120 tgcgatgtgc gacctggtgt ataacgctgg cgccggtgtg attgcggctt ctaccggaac 6180 agggcaggcc ctgcgtaagg gcgacgttgc cacactgcgg aataagttaa ctcagttcca 6240 ttatcagaac ggcaaatcac tcctcgggtt acgtcgtcgt gccgccggtc gtgttgcgct 6300 attcgatggt atgttgtggc agcaggcgga agccatcggc cgcgaagcaa agtaggttga 6360 cacataggag cattcctaag atactaaacc tgctcctgct tattcatccc tctagctcct 6420 ttatcccggt aactgaccct taccgggatt tttttttatc tgtatcccga aataatagtt 6480 gacgagtaac cataacacta ttatatttag ctcatcgaca acgagaacgg agtagagaag 6540 atgagcttac aaagcgataa agttttttac cattgcaaac cggtgcttga caccgaagca 6600 ttcaaagacg cgcaattgat ggcacgcatt gccgttaata atctgagcac acgaattcct 6660 gctgacgcgt tctggttcgc cgcgatgcag acactgaaag cagcttatgc aggggaacaa 6720 aaatgaaagt atatatcgta tccggatggc accactacga ggacacttac gttcttggtg 6780 tttatctcag cgaagaaaag gccgaagccg ccgccgaact ggaacggtct aaacaatatt 6840 atgatggagt agacgtatgc ggatgggagg tgcaggaatg agcgacaacg ggcaagtagc 6900 agtaacgttt aaggtgggtg gtaaggtagg aaatacgcca ttcccgacac gcgaggaact 6960 gttaaaacgc aatagtttcc caggtcctga ccagaacaag tatctaaatc gcatgtgggg 7020 gaagcgtaaa tgatggctga ccgcgactat gaaaagatga tggtagaggc tgtaaacggc 7080 aggtttaaga ttgaagctgt agacgctcaa atcgaggcgc accaggccgc gttagactac 7140 cttaatgagt atcgccggga gcttatcaac cgttatgacc tgaataagtg tacgcacgat 7200 tttgattaca atcctaaaga tatgagcgga cagtgtgtta agtgcggggc gattaggcga 7260 tgaaacgagt acgcaaagaa acgttgcgcc aacgcatcac gtatttggag tctgtaagta 7320 agctgggcct gtcaatgaac gaagaattcc atttagaagc atacaagatg ctgcctgaac 7380 gacttgagaa ggagagcgaa caggaatgat taccagtatc ccaaaactaa tagagacaca 7440 cggcacatta gcggatacgt gccgggagac gggacttaat gagatgacat tgtccaagta 7500 ccgccatgac acaaaatgcg agcaacatgt catctataac aaccgactga tgacccatac 7560 gaaaacgtca ccggtcatct acacgaaacg tggattatcg cgcaacgacc gtatgaagct 7620 ggaggggtga tggatagttt tcccatcctc atgtgtatag gattcacaac attactgctt 7680 gccggtgcta tatacctgat gtggattttg tttctttggc cgtttgtcga agctgtcagt 7740 cttacccgga tgacgagaga gtattggcgt attaatgggc gcaagcgagg tgcctggcgt 7800 acgatacgtt tgttctttac gtggtatgct gaaacagtat ttggtcgtag gttcgaagcc 7860 gtacatagtc gcgggtggcg atgggaagcc gtaggtaaat ggtgggttta taaagaggag 7920 agtggagaat gattgagatt ttgaaggcta ccgatgccac tggcgacctg atgttttggt 7980 tcgcctttct cagtgcgcta ataaaacaac cgactgtgtt taacagtctc ccccgatatc 8040 tgtggtggcc tatgttttta ggcttgggtg tatcaggagc ggccggcatc ctgctgtggt 8100 ttttcaaatg agattgctaa tcatcccgaa cgcctgggtt atcgccgcag ctaacgacca 8160 ttacggcggc gatggtagaa gagcactacg acacggccta tataactgac acaagcccgc 8220 atatgcgggc ttttctgtac acaggcctat ctttccctat acaatcctta atagacgctc 8280 agggggtgtc tgatgcgcta tgatggtcat acgcatttaa gagaggattg catgaaactc 8340 aaattaaaac agcctgacgc cgaagtagtg gccgccgcgc acgaagaagc ggttaacgca 8400 aaccgtagac gcaagcgccc gcgtggcaaa caaagtctgt accagtcatc caggcattcc 8460 gccgaactgt gggacccgga ctattgtgac gagttaatcc ggttcttcga ccgcacgtcg 8520 tgggaactcg tacccacgtc taaaggcgac gaacgcccgc ttatccagga caaaccgccg 8580 tcgctggccc gtttcgcact acacataggc gtaaccgttc cgattatcaa gctgtggctg 8640 cgagaggttc ctgccttcgc tgaagcatac gaaacggcgc aggccttgga agaggcgtat 8700 ttcactgaga ccggtgctgc cgggatatct gctacgttcg ccgcagcaaa acttgggctt 8760 aataaaactg ttgtggaaga agcgcgcgac gaaccaatta gcgaagtaac tattaaggtg 8820 gtgtccggtg aacgttgata taacggctac ggaaccgcaa ggcgcgttcc ttaatctgca 8880 ttgtaaattc ccggccttcg tcgctggatt cggcacaggt aaatcggagg tcatgtgcaa 8940 ctccgcccta ctcgacagca tggaaggtgg tagtgattca cttatcgcca tgtatgaacc 9000 gacatacgac ctggtgcgcc ttatcctcgc tccgcgtatg gaagagaagt tgtctgattg 9060 gggtattcgt tacaagtaca ataaatcaga caacataatc tatacatcat ccgggcaatt 9120 cggggatttt gtcctgcgta cattggataa cccggcgcga atcgtcggtt acgaatcttt 9180 tcgcgcaaaa atcgacgagt tggacacgtt aaataaagac cacgccgagc acgcctggaa 9240 taaagttatt gcccgtaacc gtcagttgcc gtgtacatat cgtccaatta ccccgaagcc 9300 agctaataca gtatcggtgt ttacgacgcc agaaggcttc cgttttgtgc acgacagatg 9360 ggccgtaaaa aagcaaccag gctatgaaat gattcaggcc tcgacaacat ccaatccctt 9420 tctaccggaa gattatgtgc agtcgttgcg ggatacatat ccgggccagt taattgacgc 9480 ctacatcgac ggcaaatttg ttaacctgac atcgggcagt gtttattacg cttatgtccg 9540 caaaaaacac agcagccggg agactataca acctggtgaa actttgtata tcggtcagga 9600 cttcaacgtc ggacatatgg cctctacgat atacgttcag cgcgattacg tctggcacgc 9660 ggtagccgag ttagtagata tgcgcgatac cccggatgtg gtcagggaga ttaccgagcg 9720 gtggaaacga cacggccacc acatcgtcat gtacccggac gccagcggca agaaccgtaa 9780 atcgactgat gccagtatgt cggatattgc ccaattacag aacgctggtt tcgagatacg 9840 ggtgaaatcg gttaaccctg ctgttaaaga ccgtgtagca tcggtgaata aagcgctgga 9900 gtctggtaga ttaatggtca acgagcaggc ttgcccggtt acagcacgtt gcctggagca 9960 acaggcttac gataaaaacg gtataccgga taagacaagc ggcaacgacc accagaacga 10020 cgcgacagga taccctattg cttatgaaat gccgttggtt aaacctgttt cccatatccc 10080 ggttactttt gcactttaag aggattatta aatgttaaca gcaaacggcc agggttctgg 10140 cgtaaaaacc aagcaccgcg aatggctgca ctacgcaccg aaatggcaga aggtgcgcca 10200 tgcgcttgcc ggagacctgg ttggctactt acgtaacgtc ggcctaaatg agccagataa 10260 agcctacggc gaagcgcgtc aggcagaata cgaggcgggc ggcatcgtat acaacttcac 10320 ccggcgcacg ctgtcaggga tggttggtag cgtcatgcgc aaagaacctg aaataaatat 10380 tccgaaggag ctggaatact tgcttaaaaa cgcagatggg tccggtgtag gcctaataca 10440 gcatgcgcag gacacgctca tggagattga ctcagtaggt cgtggcggtc ttcttgttga 10500 cgccccggaa accggcgcag ctacagcggc cgaacagaac gcgggtctgc tcaaccctac 10560 aatcgctttc tacactaccg agaatatcgt taactggcga ctcacgcgcg taggttccgt 10620 aaaccgcgta actatggtcg tgctgcgtga gacatgggag tacaacgaac cgggaaacga 10680 gttcgaaacg aaatacggcg aacagtatcg cgttctggac atcgatagcg acggcaacta 10740 ccgtcaacgt ctttttcgtt tcgatgcgga aggtggtgcg caggaagacg tcgtggagat 10800 ttacccggac ttaggggaat cattacgagg ggtaattccg tttaccttta tcggggcgac 10860 caataacgac gccaccatcg acgacgcgcc tttgttgccg ctggcagagc ttaacatagg 10920 tcattaccgc aatagcgctg ataacgagga gtccagcttc gtagtcggcc agccgaccct 10980 gtttatctat cccggtgaga acttaacccc gcaatcgttc aaggaagcga accccaatgg 11040 gatcaaattt ggtagtcggt gtgggcataa ccttggttat ggcggtagcg ctcaacttat 11100 tcaggcaggc gaaaacaacc tggcccgcca gaatatgctg gacaaagaac agcaggctat 11160 ccagattggc gcacaactta ttacaccatc tcagcaaatt accgcagaat ccgcgcgcat 11220 ccaacgcggc gccgatacat ccgttatggc cacaatcgct cgtaacgtaa gtcaggcgta 11280 taccgatgct ttacgatggg ttgctatgat gttgggtaag ccagaagata ctgaagtcga 11340 gttccagctt aacatggatt tcttcctgca acctatgaca gcacaggaca gggctgcgtg 11400 gatggcagac attaatgccg gattactgcc cgccactgct tattacgctg cgttgcgtag 11460 ggccggtgtc actgactgga ccgacgagga tattctgaac gctattgaag atgcaccttt 11520 gccgtcgggt gctgttactc aggtagtggg ggagattccg caagcggcac aacaacagga 11580 gtaaagaaaa aggccccatt acggggcctt agttttagcg gatattagcc gcgcatttac 11640 gcacaacccg cggtatcgtc tctttcagct catcccatga gttgactgct tccatatcca 11700 acaacgtcac catagccttt tgaatatcac tccgtaattg attagttttt gcatcctggg 11760 agtcccatgt atcaaacccg gttatctcac gtatatctac agattccttt atggctttag 11820 ccacctgtat gaagtgacca ctatgcccaa tcctaccctc cgtgcgctca atcattcgac 11880 taacagcagc gttaagctcg acaaagctca ccgcgccgat gttacgcagc tctacctgcc 11940 gttgtgtaat aaatcggtct ataaccagga agtgaaaata cgcactatac tgttctgcaa 12000 tgtacacggc caggtgcagg cacaaccacg tcttcccgtt cctccctctt gatgttttta 12060 atggaacaat acctgtctcc tcctcgcaca cacgcataaa gtttttgcag tcatcggttt 12120 ttagtatctg cgcaagattt cttggttgta gtccgttatc gaacctccat ctgttcatct 12180 ggatggttaa tgttgaggca tctatatacc catcacgtgt aaattccacc tcgccatacg 12240 gaaattctaa agtctgcaca ttcatctgtt tttctcctct taattgtatc gtttaaatta 12300 tattcctaat ttctttgttt acgcaaacat taatacgcaa aattatagca aattagactt 12360 ttttaggtgt ttgtgtagga ataacctatg ccatgctaag atgcatcaac aacgcaaaca 12420 ggagggatat atgccactac ttacatcatt aatcagccac caggtatggt tacaacgcac 12480 cgcatccgga gaggtgaaag acctggcgcc attcatcaag cagatgcgcg atgaggttaa 12540 gcgacaggtg ttgttgtttg gtgatgacag cagaacggcg gcgagactta ccatcatgct 12600 gcgggaactt gagcaagaac tgaacgtgat tacatcagga tggtacgaga aattactggc 12660 tgacgctcgt gagctttctg actatgaaat taactggaac gtaaaaaccc tgtcgaccaa 12720 cgttaacgct aatttcgtaa ccccagccgc cgaacaagta tgggccgccg caactttcgc 12780 gcctcttgaa ctaagcgaaa agccagttga ttttgtttct ctaatgagtg gctggcggca 12840 aaccgaggta aatcgccttg ttatgggcgt taaatctggg ttcgtacagg ggatgaccac 12900 acgtcagata gttaagaacg ttgtcgggcc tggtggactg gcggatattt ccgaacgtaa 12960 tgcggcgacg gttatccgta cagccctggc gcacgtatct aatgaagcac ggcaacaggt 13020 ttacgcgcgg aatgacgata tcatcacgaa atacgagtgg gtatccaccc ttgactccag 13080 aacctcagcc gtttgtcggt cgcgggattc gatgcagtac gaaataggca aaggcccgct 13140 accgcccgca caccctaact gtcggtcgac cacggcacca gtaatcagtc cagaattcga 13200 cttcctggat aagggtgcaa aacgggcggc caggggtgct gaaggaggcc agcaagtaaa 13260 cgcgggcacg acctactacg agttccttaa acaacagccg gcgtggttcc aggatgaagc 13320 acttggtcct gtcaggggta agatttttcg caatagtggg ataaccccgg aagaatttcg 13380 tgtaatatca gtagatggat tcgggcgtcc gttaaccctt aaagagatgg cggagctcga 13440 taagcgtgtt gccaaatacc tgaaagagga ataaagatgg gctttttcaa agttaaagat 13500 gtgccggcgc gtcgggttgt tcagtactca cgtgtgtctg gtgctggcga aggtgtcgtg 13560 tacattaaag atgaatctgt tcttggtgag tcggtagatg aaatgccgtt cgccgataag 13620 accgggctga ctgcaatctc agacggtgtc ctgtacgagg tgccgtatct tgacggagcg 13680 ggtgacgtgt actttgatac acaaccggta gacacaaaac tgaaagacgg ttccgctaaa 13740 ctaatcgtag tcgtgaaagg tggtaaggct ccttacgatc tgcaatggtt taaaaatggt 13800 aaagaggtga tcaacgtccc gtacgttgag ggtgaactaa ctgttaaaga cccgggggag 13860 tattttgtca gggcggtaga tgccgacggg atatcggtgg taagtaaagc ggctaaggtc 13920 tcagaaccta agtaacgaaa ggccccgcca aggggcctta tttttacaca ccattattcc 13980 agttattcct taccaatgta aattcaggaa tactttattc aaaaagtaga cttctattat 14040 tttttatgtt acgctaccta cgctttcagc gtaccgcggt gcggacgcgt gaagcgggcg 14100 ccacagcgcc ctagcgtaac cgcgaacgca ttcggaaggg ccgagctata ttgctttcag 14160 tatttatcta agattcgccg tatgatgttt acagatatac gagtttaacg cccggcttac 14220 gccgggctta gcgtataaag aaaggggata ctatgaattg gaaagtaacc gcagtaatca 14280 cggcagccgg catcctttcc ttgtggttat acggccagta caactacaga agcgggtggg 14340 tagaaggccg agagaatctt gtttcacaac aacaaaagaa ggcacaagct gagttgacga 14400 agaaaacaca acggcagcag cagaatgata ctaaggccgc ctccgccgaa tcggaaggca 14460 aagaaaattc ggaggctatc acccgtgaag ttatcaaata cgttacccgt cctggccgta 14520 ctgtctgtga gtttcctcct gaacgggtgt caatcaaacg acgcgccgcc gagaatgcta 14580 attccatccc tggatatgac actaatgcag ccgccgtgca agcttattcc accgagcagt 14640 gacgctgatg aagacctggc cattgacgta cgtaacgcgg agtgcacacg gcagttacgt 14700 ctgaaagtgt tccggttgca ggagtacata aggaatattc tggaatagtt gccttagtaa 14760 gtggaataat ttattcttgt tacagaaaca ccgggtggcc cggtgtccta aagtccaggg 14820 gacatattga ctatgaatcg ttttttacac tatccactcc aggaagaagc tggggtagaa 14880 gataaatcgg gtgcaggtga tgcaccaaaa atgtacaccg ccgaagaagt gcaggccttg 14940 attgagaaag aagtggccgg gctcaaggca aatcaggaag cgctccttag tgagaaaaag 15000 gaagctgctc gccgggctaa agaagccgaa gaagaacggc agcgcgccca ccaggaggcc 15060 ctgaaggctg ctggtaaaat ggacgaattt gaaaagacga ttcgtagtca gtatgaaccc 15120 gtactgaaag agaaagaaga acgctacgct tctttagccg cgcgaattct tggaagtgaa 15180 cgtaaggctg ttttgggttc tttcgccggt gatttcatca ccccggaagc ggtagaaatc 15240 cttgctccgt tcgttaagac tgaatttgaa ggtgaagacg tagttactaa attcacggat 15300 gcggatggta acgtagttac taccgaccct gaacagttcc gcaaatacct gcgagaacat 15360 aaagcttttt cgcatttgat taaagcaaat gcagcttccg gtggcggggc ttccgggaat 15420 aaaggcggcg gggccgcacc agcgtttaaa gatatgagtg aaagtgagcg gttggctctc 15480 tataaatcta accctgccga atttgaacgg caacttaaag ccctgaggaa ataaataatg 15540 gcaatcacca ctattggtaa catcgtaact ggcaatatcc ctgtactggc gtcctatatg 15600 acggaagacc cggtagagaa aaccgcgttt ttcaactccg gaattttgac cccaactccg 15660 tatgctgccg agattgcccg cggaccgtct aacatcgcta acctgccgtt ctggaaagct 15720 atcgatactt ctatcgagcc taactactcg aatgacgtat accaggacat cgcgaccccg 15780 cgcgctattc agaccggtga aatgatggcc cgcgttgcat atctgaacga aggtttcggc 15840 caggcagacc tgactgtaga acttaccagc cagaatccgt tgcagtccgt cgcatcccgt 15900 ctggataact tctggcagcg tcaggcgcag cgtcgcctta ttgcaactgc actcggcctg 15960 tacaacgaca acgtatccgc tactgatgcg tatcacgagc agaacgacat ggttgttgac 16020 gtttctgcaa cttcaggctt cgacgcaggt gcattcatcg atgctaccca gactatgggt 16080 gatgcattga tgggcaacgg cggtgaggtt ctcggtgcta tcgcaatgca cagcttcgta 16140 tacgcacagg cccgtaaagc ccagcttatc gacttcatcc gtgatgctga gaacaacacc 16200 atgttcgcca cctaccaggg ttaccgcgtc atcgtcgatg acagcatgac cgtagttggt 16260 caggacacta gccgtaaatt catctccatc atcttcggcc agggtgctat cggttacggc 16320 gaaggcaatc ctgagatgcc gctggaatac gaacgtgaag catctcgtgc taatggtggt 16380 ggtgttgaaa ccctgtggac tcgtaaaacg tggttgctgc atccgtttgg ttacagcttc 16440 accagcgctg taatcaccgg caacggcacc gagactaccg cccgctccgc tagctggcag 16500 gacctggcta acgccgccaa ctggaaccgt gtagtcgacc gtaagcatgt gccaattgcg 16560 ttcctggtaa ctggcgtagg tgcttaatcg tagggtatac tcttgaggga cttcggtccc 16620 tcttttcatt tatacggaga ctacataatg tcaaagacag gtaaagggtt accccgcagc 16680 ctggttaacg cggaattcga tattcccgcc gctaccacaa cgacaatcgg aggggttaaa 16740 aagtccgcta cagtagccgc accgccagcg attagtgccg gtagtggcgc agccgcagca 16800 gccgctccta cgaaagaaga gtttgacgcg ttggtgtcta actacaataa gttgcgcacc 16860 gatgtaacct cattgcgcac tgccgttact aatctgttaa ccgcccttaa aaacgctggt 16920 actgtatcct gaggaggata aaaatggttg atgtaattaa acgtcgtatt gttggggtat 16980 ctgatgacag cccgcaggac gggcaggtag agattgatat ggaaaacgtg atgccgttgc 17040 gtttctctac tggtctaaat gatactactg cagtaaccgc aggtcaggct atcaccctga 17100 ccgttgtact cgctgacgga atggacccta aaacagttca atggtataaa gataataacg 17160 caatctccgg tgcaaccgct ttgacttaca ctaaggccaa ctccgcagcg gcggattccg 17220 gtacttacaa agtcgtagcg cacgacggtt acggtaatat catttcagat agtacggtag 17280 ttaccgtaag ttaaatacac gcggcctccg ggccgcttta aggattagac atgacagata 17340 attacgtagt acgggaacaa tacaagggtg tggtcgaggt tgacgggcag ttagtcccga 17400 tgcgcgaaga agcgaacccg gaagcattaa tcgaaactca gccagtggcc gaagagccgc 17460 attacaacgg cggcggagaa cctaagcagc gtcgtcgccg caaaagcgta gaggaataaa 17520 ttatgccgct tatcgtggaa accggtcaag gcatccagaa tgctgactct tacgtcagcc 17580 tggaagacgc gaggtccatg gcctctaagt acggtcttga gctgccagaa gatgatgcta 17640 aggccgaagc ggcgctacgc aacggcgcgg tatacgttgg gctttttgaa tctcagatgt 17700 gcggccgtcg tgtatccgca aaccaggcgc tggcgttccc tcgaaccggt gttactttac 17760 acgggttccc tcagccatcc aatgtaatac catcgttagt tgttcaggct caggtaatgg 17820 ccgccgttga gtatggcgcg ggtaccgacg ttcgagggtc tacagacggg cgagaggttc 17880 agaccgaacg agttgagggt gcggtaaccg tgtcctactt taaaaatggc tactcaggca 17940 gcacagtaag catcacagca gccgatgatg cgttacgccc tctcttatgc ggtagtaata 18000 acgcctactc ctttaatgtt ttcaggggtt aatcatggct aagactaaat cagaaatatt 18060 ttcccttatc ggtgccaact tccctgataa ccagtctgga ctaattacac cagaaaagtt 18120 gcgcgaagtt accacgcaaa tcgcggattc tatgctttac ggcgttaagg aagtagaagt 18180 acttcgcgcg gcgtctacgg atatccaggc gccgaccacc acaggcacgg cattgactgt 18240 agcctttggt ggagcgcaga aaacaagcgc agaccctgta atgataaatg catccggggt 18300 agttacgttc aacgccgctg gtaactacgc tatccgggtt aaattgcagg ccggtcgcac 18360 cggagcgagc ggaacatcca tccttctgtc gcgtgttctt ctcggaggtg cgcaattcgg 18420 atcacccgcc gcgactaaac ttgcgagtgc ggattccaca attcccattg aatcccgtgt 18480 tgttgttaat gccacctcag ggcagacttt tacggtagag attatgcgtg atgccgccgg 18540 ttccaacttc ggcgggttat acccgcaagt ggcaacggtt acgtcctggg gtgtggcccc 18600 atccgcacta ctggtcgttt caagactgga gggtgtgtga tgagcaccgc ttttagtaaa 18660 cggatgcaag gcgtaggtac tcgcctactg tcaaaatacg gcagcacggt aactttggtg 18720 cgcaaaggcc agaaaacatg ggaccctgtt ttaggggagt acgtgtgggg gcctgatgac 18780 atacttcctc ttaaagcggt tcctgtaccc gttaacgccg gtcttgtaaa cgggaccacc 18840 attcaggccg gggacatgat ggttaaagcg gattacagcg tcgttccgaa gatgggtgat 18900 aaggtgcagt ttagcggaga acaatggtct gtagttgcta ttgagaggaa gatggttaac 18960 gatgatgttg tggcatactt tattcaggta agaaaatgag ttttgcgctt gatgtgtcca 19020 agttcgtgga aaaggctaag aagaatcctg agaaggtaat gcgtcaggtt tctataaagt 19080 tgttttctgc gattattaaa gcaagtcctg tcgataccgg gcgatttcgt atgaactgga 19140 tggcttcagg aagcaccccc gccgatggga ctacggacgc taccgataaa tcgggcaacg 19200 cagcaaccgg taaggttacg agtttcgtgc taaacgctgc tgactggcat accctcaccc 19260 tcactaacaa tttaccgtat gcacaacgtc tggagtacgg ttggtcgcaa caggccccgc 19320 aaggtatggt caggactaac gtcagcaggt tccagcaact actaaatgaa gaagcctcta 19380 aggtgaaata atggcaacat attttgagga cttaacgaaa gcattcgact cggcgctggt 19440 gacgttcggc atggacaacg acatcaaggt tgcgttagag aacatcgatg cgccgacgtc 19500 tactgatatg ccgtatcttg caagttatat gctgttgtcc gatacggagc aagccgactt 19560 gttctggact gaacaacggt caggtgttta tcaggtggac attaacgttg ggtcagctct 19620 aggtagtgcc cctatcaacc gattagctga taagctaaac gctaccttcg cggccggtaa 19680 ctgttttagt cgtaacgaaa tctgtgctga ggtacaatca gtaggcctcg gtcctcttat 19740 tgttgagaat ggatgggcga agaggcctct ctcaattaat ttcatagcct ttacagcgag 19800 gattagataa tggcgttaca accatataaa ggcgcgatga ccgcgcaatt ttacgtactt 19860 gagacgacgc cgggggtgac gcccaataat ccggtgtggc agccgctccg caacactggt 19920 ggcattccgg ccgtaacgcg tgacgccctc atctctaacg agctggacgg cagcagcgaa 19980 acatcatcta tccgcacagg taaccgacag gtaactggtg aatacgctat tgaacttagc 20040 gcggaaagcc aggatgagtt gcttgctggc gcaatgtcca gttcctgggt agcaggttcc 20100 actaaatcgg gaatcagcgt taccgtagac ccggtggcga aaacttttac acgggctacc 20160 ggtagctttg taaccgatgg tgttgaagta ggtgacctgg tgcaattcga tggtctgtcc 20220 ggtaataacg ataaagcttt cctcgttacc gcggtaaccg ctacggttgt aaccggtgct 20280 ggcatccagc acactctaac cgcccaagca gacgcccaag ccgatttgcg tatcgcagat 20340 aaactggaaa ccggtaactt gtgtaagacc tattcgattc ttacgtggtt gaaaggtaag 20400 tgcggaaacc cggattcata tatcgtaacc agaggcgttg agtttaccgg gttcactatc 20460 gaacaggctg ttaacgcgat ggtaaccggt tcgttcccgt tcatcggctt gaatcaggaa 20520 atcttacagg cgccgcctag cggttccgac ttcacgacta attttagggc ccgcccgttt 20580 gcatcagttg atgtatctac ttatgacggt gccgcgccac ttaaacttat cgacacgttc 20640 accattacta acgacaacgg cgcatccgca cagttcgagt tagggaataa tagcgtggca 20700 tttgttgagc gcagtcgtgc ggccaacacc ttctcgctgg caggtaaact gtacgacctg 20760 acgctgttaa ataaattcct ggatgaaaca gaaatggagg tatcttcggt tcttgatggt 20820 ccggatggcg ctatgagctt tacactcaaa cgtgcttcac ttacgtccgc aaccccagaa 20880 atcggcggcc cggagtctat taccctttct cttgagggac aggcaaccgg taaccggttc 20940 cagtcttcaa ttgttatcca gcgcattaag tacgcctaag aaaaaggccc cgaaaggggc 21000 cttagtttta aatcaagcct tctgccgtta acttaccgat aatccacatt tcaccttcag 21060 cgaagaacat agcctgtgaa tgcccagttt ctgtttcacg cataatgccg taacccttat 21120 cgatgaacca ttgctggaag acacgaccgc gttttacgga gcgattgtat acgccaaatt 21180 gttcaagttg tttattcatc cacacggcag attgaccgaa cttctgggca acctgcgtcg 21240 cgttgtacaa gttatttctg tcgatgatgc ggtcgtagac atcagctttt ggtgccattt 21300 ccttgttctc aagcgcaagg cgttctttct cctcccacaa gtccgcagcc aaccttaaag 21360 cctcccccat tgtcttgggc actggtaagg atggtttgct cgcttgtgac tccagttcct 21420 gccaacggcg gatgatggca gcgcgcattt tgatgctgta ccctgaaatc aggattaagg 21480 tattttcttt gtctaagaga aataccttac ttggtctgcc accgtttgaa ggcaaaacct 21540 tttcctcaaa attgagggaa agttcctgtg ccatttttaa tatatcgcgt cgtacgttgt 21600 cgtgttcttt ccccgtcaat tccgcaatct cacggctgga catggtttgt gcttcactaa 21660 cattcattaa ttcgttcatg gtttcatctc ctcttgttgg cgtagatata tttaacttcg 21720 aacattctta tcgttcaaat cagaatcttt atcgtgacga taaaaaaaag gcccctttcg 21780 gggccttttt ctcactcatc aatattacta cggtatttcc gtaacttctc cagactttca 21840 attgcttctg ctaagtcggt tcctgtgtcc ttgtgccccc gcaagcccat acacaacagt 21900 ttcttcaacg catgctgtag tgccgggtcg cggatatcaa atgccctcag aacgtcgtac 21960 acatcacagg taatgctatc tccgtcagta tttgtcattg tgcgattgta tttgttagtc 22020 atccaagcat ctccggtgaa atagttaaac gcgcgacttc gccgtattcg gcactgtaag 22080 taataacatt cgcgctgcgg cctgacatcc atccaccacg tgatgcgtaa gcatctttcg 22140 ccgcgagagt gcggtgttgt tcaacaatca tgttacggct ttccacaatc ttctgatggt 22200 gaaggtggcc cacatgggcg taactgtaaa cgctctctcc gaacgcctta cggaacttgg 22260 cgatcataac tggttcgata gcatcgaatc tcgccttatg cccatggtga aagaaaaggg 22320 tcgtcttacc gtgttggacc atcttgtaga catcagccga tgtatctaca aaaacacgag 22380 gttcattatc gtaaagagtg ttaaacattt cagccaacca aatcatgcct gactcgtcat 22440 ggtttccttg cacaattagg agatgaacat ttttatgctt aacaagcgcc atattaacca 22500 cgcgcctgac catacgaatc atgtagcgca ccagtttctg gtaacgcgtg tccgcgtcca 22560 gtacatggcc actggcaggc gtaacggcat caaggctgtc aaaatgcgca aagtccccta 22620 ataggttaat aacacccaca ccggcatcag gcgccttctg aaacgccgcg tcgaaccatc 22680 tcgagaacag gtcttctgca atcttcatat cccagtcgtc gccactctca tccgcccagg 22740 ccaacatacc taaatggaag tcagaaaccg tgtaaagatt tagcaacttt tcgtttagtc 22800 gctttttagg ccgagggacc gaagaaactg gcgtaatatc ggacttcatc ccatctatta 22860 cggctcgcat caactcgacc tgacgctcgg cgtcggcgtc agtcttaacc cactgaagtt 22920 tcgtgttacc gaactcatct accagtgacg acgtaccctt aatcttatac ccatctggca 22980 caaggtggct tacgtcgcgc ccgtggccaa ctcctttctt tgccaggcgc gcggaacgaa 23040 gctgaacatt acggcgggac atattatact ttttggctat ttcagtaggg ccgaggccag 23100 catttagctc ttgctgcaac tgttcatctg ttattttgcg agtgaccatg cttgtttcct 23160 gactgtttaa gataaatctg attctaataa cgtaagttta atttagccag attatttacc 23220 attaacatta gagttacagg cccagatgag agccccaacc cacggcagaa gaatccaccc 23280 cagtaaaaga ttacatacga atattcctaa tttcgcttta tgattacgta gcaaggctac 23340 caggaacggg atgaaatagg taaacgcgat aataccgata aaaacgctca tgttcttaac 23400 ctcatattgt ttagttgacg tagagataat aggacactat taacggttgc gcaagctatt 23460 ttgctattct tatttagcgt ctacggtcgc gccagaaaag cgggtggttc ccgcctgacg 23520 caactatcca accagtaacc gactaaccaa agggcattac tatgaaactt agcgattttt 23580 actacgaagc agaagccgag aaaggcgcgc gtatgccgat tcctttaaaa gatggtacag 23640 attcagggga atggttgaac gttgtctctc ctgaggctga tgttgcggtt aaagctatgc 23700 gggccttcac cctggcgtac cgagcggcgg taggtaaatt aaaaccgctt cgagataaat 23760 gcgaagagca aaaagacttc tcagaataca atttaaaaat ggaagacgcg gcaggagacc 23820 taaaccggca attggctctc gaattggtga atggttggag tctcgatgat gagttcacta 23880 aggaaaatct tgagactctt ctcacccaat ataagcgcct ggcggaacat gtagtcgtat 23940 tccaccacga acagttgcgc caattgcagg aaaagtagac gcgttgtttc agttcgcccg 24000 ttggaacttc ataacccgcc acgaaaggcg caagtttgac agtatagccg acgggcacaa 24060 agccgcgctt atcgctatgg gggtaataga agacgcggaa gaaacaacgc aggacgccgg 24120 gcctgaatgc ccccctgaac tactaaccac ttttgagaag tatcgtgatg ttaaatttac 24180 ccgccgcgtc gatgacgacg gcgtaaagct atacccaaga gagcaactta gttggtcaga 24240 tttagtggcg tatagcacta tttcaggtca gaatataggg atgtttgaat ctgaaattat 24300 catgggctta gacgccattt ttgagggtag aaacgatggc tgatgtagct agcttagtag 24360 taaaggtaac cgaacaaggc gcgaaggcca catcagaccg tcttgataac ctctctaaat 24420 ctgcaaaagt tgcgggggcc gcggtcaccg gactggctgc cgttgtagcc gccgcagcct 24480 ataaggcggc tcaggaacta gtcgagtcac aacggcaact ggataagatg tcggccagcc 24540 tgaaaacact aaccggaagc acacaaggtg caaaacaggc cctgagtata ctacaggact 24600 tcgcccgtga cacaccttac ggccttgaac aggcggtaga agggttccgt aaactggtag 24660 ctctaggcct caccccatca gaagaagcgc tccgctccta tggcaacacc gcatcggcta 24720 tgggtaaaga ccttaaccag atgattgagg ccgtcgcgga tgcgagcacc tttgaattcg 24780 aacgtctgaa agaattcggc attaaggcca agcagaacca aagtgatatc gaatttacct 24840 tccaggggac gactactgta gttaagaaaa acgctgccga tattgagcga tatcttctca 24900 acataggtaa cgttaacttc gccggagcga tggctgacca ggctaatacc ctggacggag 24960 ctatcgctag tgcggaggac tcatggtccc agttgaagat gactctcgat actagtctgg 25020 atgtagggtc gctggcggag cctttacgtt atattgacga cctgatacag gagataaacg 25080 ctcaggtcgc atccggtgag ttcgtagccg agatgcggat gtggggtgac atggcatccg 25140 atgttggggg tgcgatagag gcctcattcg acgcggcgtt tgggatggtt gcagacgcgt 25200 taaacgcttt gaactccgcc tggacttaca ccagtgaaag cattaccgga agcggagagg 25260 agaccgcatc tacgatagct gagtcagcgg cggatgcgct ggacttcatt gcccaggaat 25320 ttacagcaat ggagcggttt tttgaagata tggttaaagg tgctcaggat gcggggcgtc 25380 ttgtaaaggc tgcattgacg ccgggagagt cggtagctga ggccaagaac cttaacttcc 25440 agttagcgct tgctatggat actcaaaggg gtgtggctga cctgacacgg aaaagtttcc 25500 gcgaacaagt agaagctcag gaagacctta tcgcgctgaa gcgagcggct tacgacatcg 25560 ataaagaagc agctaaggcc gaggggttag gtaagtttaa ggtatcaggt aaggacagcg 25620 gttctacagg tgattccgca gacaaggccg ccaagaaatc catcgacgca ttcgaacgcc 25680 agaagaaagc cgctgaggat ttctattatc agtcaatcca ccttaacgac gacgtattcc 25740 agaagataca agctaaccaa gaagagcaac ttacaaagct acaggagttc tacagcaacc 25800 gtctccttag tgaccagcaa tacgaaaccg ctaagacgca gattatgctc gaggcggata 25860 cggcccgcca ggctgaatta gataaacgcg agaaagagcg cctggaaaaa cagttctccg 25920 cagacgcgta cgtcgctcag atgcaagccc ttgctgaagg agagttcgcg gagttagacc 25980 gccagtacga ggttaagcta caaaaactta acgattttca tgcgcagggt ttgatcgccg 26040 aagaaactta ccaacaaacg ttaagcgaca tggatgaatc ttactccctt gaccgagtga 26100 aggcgaccgg cgcggctttc ggtaacatgg ctagtaacat cggggctgcg ctaggagagg 26160 cttccacggc atacaaagca tttgcaattg ctcaggcgac catagctacg tacacatcag 26220 cggtcgaagc gtataaatca acagcagcca taccggtagt cggcccgtat ctggcgcctg 26280 ttgctgcggc tgctgcggtg gcggctggtt tagcaaacgt aggtaagatt cgctccgccc 26340 gagaacaggg tggtaacctg gccgcagggc agatatccac tattgcagaa cgtggtaaac 26400 cagaagtaat catgcccgct agcgcctccc gtgtccggac ggcggagcag atgcgacaga 26460 ttatgggcga gaatggcact aaatcaggtg gggataatgt tactattgta aacaacacca 26520 ctggaagaat tgattcggct gcaacagaac gcgacgacga aggtcgttta cgtattataa 26580 tcagtgaaac cgtaagttcc gcgctacagg atagtaacag cgctatttct aaggcacgtc 26640 gcgctacacg cggccaacca ggatattgat atgagcgatt accatttccc ggcctctttg 26700 aggccgatag tatcgaaagg atactcgatg actcgcggca acaacgtgtg gcgggtagac 26760 ctggccggtg gcggagttcg ccaggggcgt gatacatact ttgatgtgtt cccgattagc 26820 gttacgctgg tcgtgtcgcc actgggtaga caagcattcc tcagtttcat ggagaaggta 26880 gacggagggg cttccagttt ctggatgaaa cacgaccttg gccagggtat tgacgattac 26940 caggtaacgt taacgtccac gtggaacgag tccacagatg acggaaagaa ttgggtaatc 27000 accttcacgg ccaccgccga gaagtcgcca ttccaggaag ccaacaacgc ttgccttaac 27060 cagaacctac ccgatttgta cgggtgctat ggcgattgcc ttggcgaatt ccttaaaacc 27120 tatggagtgt atcagactac attccctcga atctgggacc ctatgcaatg agtcaggaat 27180 cagtagaagc ggcataccgg cgtaaactgg cgtccaatcc agacggtgaa atggatttta 27240 ttactctcga gatatgccac ccacttcttt cgaaacggtg gttacttgtg cgcggagtta 27300 aagacttaac cgcaactctt gagaccgggg aggtagtgac atttgaaggt acgccgatgg 27360 aggctaagaa cgccgctaac aataacgata tggaccagac cgcgtccttt tcgctaccgg 27420 atgtgcttaa catactggat gaggaaatgg accgcatccc ttatgacaat aaggaactgc 27480 ctaaattcat cttccgtcgc tatgtgagta ccgacctatc ctatccatgc gacggcccgg 27540 tggtttatga gttgcaaaca ctcacacaag aaaaaggtgt atttacggcg gaaaccggta 27600 cgccgatgct taaccagcgg gctaccggta ttttgatgac gccggaggag attcctttac 27660 ttcgcgggat actgacatca tgaatattaa tgattacact ggcttgccgt atgactttcg 27720 ccgtcgtaat tgctggcatc acgtccgcaa cgtccgcgcg gatgctgggt tatcaactcc 27780 aatgtttgac gtcaccagcc caacggcaat agacgccgcc ttcgacgatg gccactctaa 27840 tccgaaaggt ctggtacgag cggtcacacc gcagaatttt gacgcggttc tactaggagt 27900 gaaacatagg gggcgaatag tgtggcatgc tggggtatat tacgaaggaa tggttagcca 27960 ctgtgagctg gcgtccagac aggtcagact ggatagcctg gaagacctta aagatactta 28020 ttcggagatt gaattttggc gctagtaatt cactacaccc gcaacgaaga cggcacattt 28080 gacgctaaac gttatcacga taatccgatg aacttcgtcg tgaaccatgt tccggacggg 28140 gttccggtac gtgttttcat tgacgaaatc ggagaagata acgacgtaac agaagacttc 28200 gaagcactga aagaaaacgc gactttccac attgtagaat ctgccggtgg gggggctatt 28260 aaaggcgtca tgaaggtttt tagcgttatc cttaaaccgc tggcgaaact actatcacca 28320 tccgtgaaag gggcgtcctc taacctggcg aactcgcagg cggattcccc gaacaacagt 28380 ctcaccgacc gcaacaacaa ggcgcgcccg tacgagcgca gttacgacat ctgcgggacg 28440 gtgcaaacca tccccaataa ccttatgact acttacaagg tgtttaacgc cgccggtaaa 28500 attgtagagt acggttatta cgacgccggt cgcggctatc ttgacatacg ccctgaagat 28560 atcactgacg gggacacccg tgtatcggat ataacaggca cgtcggttgc cgtgtacgcg 28620 ccgtatacgt cacccaataa cacgtctaca ccccaggtca tggtcggaga cccgatagag 28680 cagggcctgt atattaccgt agaatctaac gaagtcgatg gtgtggttct taaagcaccc 28740 aacggtctgg gtatttcttt ctcttacatg tccgggtatc cgtctttgtc cgggaacatc 28800 ggcacgatat atgacccaac aggtggttcg gatttttctg gagtgttggt gcctaatgac 28860 acgttttcgt tggtgtcagc gtggacaaat acggacgtcg acttgtccgg cggcgggtat 28920 caggtgttaa gcgtatccga agggaccgtt acctttatcg tgcctggcgg ccttatcggg 28980 cgatggcaag aaataagacc cggttcattt ttccgcggtg atggagaggc ctcgctgcaa 29040 ccagacaaca cgtatgagaa aacattaacc gattgggttt caataaaccg taccgaggtt 29100 gagcgtatag tcgccaatat cgccgccgcg aacggcatgt ataaagacaa cggcaaatcg 29160 aaaacactgg cgtcagtcac cgccgaaata cagtatcaat tgctcgatga aaacagcact 29220 ccttacggac cgatatatac tgcgcaagga actgtgtccg ggcgtacacc ggactacaac 29280 ggcgtcacta tttacgccga cctgccggtt gtgtcacggg tgcgggtgcg cgccagaagg 29340 gtaacagacc tggactttaa tttcgagggg tctgtagttg atgagataac gtacgttaac 29400 ttatacgggc aaacacgtga caacacccca cactacggca acagaacaac agtacactcg 29460 atgcgcaagc agaccccacg tgctgcggaa gtaaagcaac cgcagttgcg tatgattgct 29520 actgaaatgg tgtacaaata cctcggtaac ggtgttttcg aagatacgat gacacctaat 29580 acacaggctg tacaatccct catccgcctg gcgcgtgacc cggatgttgg cggtttaaac 29640 ctgacagtac gcaacatgga taagttactt gccgtgcaga acgaggttga agcgtatttt 29700 ggcgacaaac aggctggaga attttgttac acgtttgatg actataaaac cactatgcag 29760 gatatagtta gtactatagc cgacgctatc ttctgtactc catataggcg tggggcggat 29820 atccttctcg attttgaacg ccctcgcatg ggtcctgaga tggtgttcac ccaccgaagc 29880 aaggctggta cttccgaaaa atggaccaga acctttaacg atgctcaggt gttcgatagt 29940 cttaaattct cgtacataga ccctaaaaca aacgttaaag aaaccataac aatacctgaa 30000 accgggggcc ttaaaacgga gacttatgac tcaaaaggaa tccgcaacta taagcaggct 30060 ttctgggcgg caaaccgtcg ccaccagaag aacattttaa agaaaatttc ggtgtcgttt 30120 accgccactg aagagggtat ttttgccctt ccgaatcgtg ccgttagtgt ggttaagggt 30180 tcgcgtatgg ctacttacga cggctacgta accgcggtta acggtcttac cgtagagcta 30240 tcccagccgg ttaagttcac atccggagat gaccattctt tgattctcaa gttacgtgat 30300 ggtggagtgc aaagtgttaa tgtagtccct ggagcgcacg acaggcaggt aattatgaca 30360 tcagtaccgc aagaagccat ttacacaggt aatagtgctt tgaaaactga attttcattc 30420 ggcaacgaag caaggcataa tgctcagatg attcttgttt ctacggtaga ccctggcgat 30480 gacagaacag tcaaaataac cgggttcaac tatgacaagg atttctataa gtttgacaac 30540 gtgcctcctt tcggtcgtgc gttctccaac ggattcgata acggttttaa ctaagaggat 30600 agccatatgt ccagtggttg cggtgatgta ctgtcactta acgatttaca aatagccaag 30660 aaacaccaga ttttcgaagc cgaggtgatc accggcaaac agggcggtgt agcaggcggc 30720 gcagatatcg actacgccac taaccaggta accgggcaga cgcagaagac gctgcccgcg 30780 gtcttgcgtg acgccggttt ctctccggcg tcttttaact tcacaaccgg cggaaccctg 30840 ggaattaacg acgccaataa agcggttctt tggccgaaag aagatggtgg ggacggtaac 30900 tattacgcat ggcgtggctc cctgccgaaa gttatccccg cggcatcgac acctcttacg 30960 acaggcggca tttctgattc ggcttgggtc gcgttcgggg acatcacctt ccgtgcggaa 31020 gcggataaga aatttaagta ctccgttaag ctgtccgact ttactacgtt acagcaactg 31080 gcggatgctg ccgtcgacag tattcttatc gaccgtgact acaatttcag caataacgaa 31140 accgttaact tcagcgggaa gaccctgacc atcgactgta aagcgaagtt tatcggcgac 31200 ggaaacctgg tatttacgca attaggtaaa ggttccattg taatagcccc ctttatggag 31260 agtgctacaa cgccgtgggt gattaaaccg tggactgacg ataatcaatg gataaccgac 31320 cccgcggcaa tcgtggccac acttaaacag tctaaaacag atggatacca gccgacggta 31380 aacgattacg ccaagttccc tggcatagaa tcccttctcc ctccggaagc taaagggcaa 31440 agcatatctt ctaccctgga aattcgggaa tgtacaggcg tcgaggttca ccgggcgagt 31500 ggtcttatgg cgtgtttcct gttccgtgga tgccatttct gtaagatggt agacgctgac 31560 aacccgagcg gcggtgcaca cggcgtaatc accttcgaaa acttaagcgg agattggggc 31620 aaaggtaact atgttatcgg cgggcgcaca agttacggtt cggtaagtag cgcgcaattc 31680 ttacgcaaca atggcggttt cgcgcgcgat ggcggggtca tcgggtttac ctcgtatcgt 31740 gcaggggaaa gtggtgttaa gacgtggcaa ggtacggtag gttctacgac atctcgtaac 31800 tacaacctgc aattccggga ttcggcggta ctgtaccctg tatgggacgg cttcgattta 31860 ggcgcagata ctgacatgaa ccccgaagat gaccgcccag gggatttccc catttctcag 31920 tacccggtac atatgctccc tttaaaccat ttgatagaca atctatttgt tagaggttcg 31980 ctgggggtag gattcggtat ggacgggcaa ggtctgtatg tctctaacat aaccgtcgag 32040 gattgcgctg gttctggggc ttatattctt gcccacgaaa cagtattcac taatatcgca 32100 ataatcgaca ccaatactaa aaacttccct gcgaaccaga tatatatctc gggggcctgt 32160 cgtgtaaacg gccttcgttt ggtcggcatc cgttcaacta ccgaacaggg catgacggta 32220 gacgcaccta actccactgt aagcggaata acgggcttcg tggacccctc aaggattaac 32280 gtagccaatt tgatggagga aggtcttggt aactctcgca taaacagttt caataatgat 32340 tctgcggcgc ttcggtttcg tattcataaa ctgtcaaaaa cccttgatag tgggtccgtg 32400 tactcccaca ttaacggcgg gccaggttct ggctcagcat ggaccgaaat taccgctatt 32460 gcggggagct tgcccgatgc cgtatcatta aaaataaata ggggcgatta tcgtgctgtt 32520 gagataccgg tagcgacgac cgtcctacca gacaacgctg tcagggataa cgggtctata 32580 tcactgtatc tggagggcga tagccttaaa gcgttagtta agcgggccga cggaagctat 32640 acaagattaa ctttggcata aatagtaaag gcccctttcg gggccttctt caaattacgc 32700 tgtacaaagc gtacatagta aaaacaccgg caataataag tcctgctacg aagcattttg 32760 ctccggaagt gtatcgcatt tcagattctc cataataaag tcaagttgcg cgttcgccgc 32820 gtctcgctgc tgccgtagtc tggcgttctc ggcttcgagt tccttgattc tatcgtataa 32880 atctttccaa gatttaccaa tgtaagttcc catcacacac ctctcttctt gccgtggttt 32940 tcatggaatc cgtatttaac ttcggcagct ttacgctctg ctacagcatc ggaaaacttt 33000 tcaaattgac cgagatgtat ctttttacgg tctaccttta tttctgcgac ccacttattc 33060 cgtcgcttct cgaaccagac tccgagtgcg ccgctcgtat tgtttacacc tattgatgcg 33120 ttgcggctat tttcagtgtt ggtaactttc cgcgggttgc atattcggtt atcagttcga 33180 atatggttaa tgtggtctat ctgctcctgt ggtgtaagtt ggttatcggg attgcaggca 33240 ccccatgcta accgatgtgc tagttctagt tttccgttta ttcggataca aatgtgccca 33300 tccctcggac aaaagcatcc cgctttcttt ccaataaaac ccctacgttt tgtttctacc 33360 catgtgaaaa taccggtttc agggtcgtaa gataccttgc tcatttctta ctcctacgtt 33420 tcatataatt aagtagttcg tcctgtacag accttttctc gtccgtacga gcggcgacaa 33480 cttcatcaag agtatctcgg gctattattt tataaatgaa tacagaccgt ttgtacccag 33540 attgcatctg ccttaccgga cctatgcgct cgataacctg cagataatgt tccaggttcc 33600 acccctgact cataaaggcc atgtggtgcc cgccgtgttg tagcgacagc ccgtgacccg 33660 cggaggctgg gtgtatcagg agcataggta tctctccgcg gttccacgct tccatctgct 33720 tattgccctt ggcacctttg gcgaacgcct gcgcctgtgg gaaacgctta agaatgcgct 33780 caagttcgtg cttaaactga tacgccacca gcaacggcgc gccctgcaac tcctccacaa 33840 ttgactccag tgcgtccagt ttcgtgtcgt gcactttctc ccagtctttg gttgcttcgc 33900 catctggccc tgatacgtat acagcaccgg aggcaatctg gaggcatttc gacgttttag 33960 cagcggcatt agccgcttcg acttctccgc tttctagctc cgcgaataac ttctcctcca 34020 tatcgatgta agcctggcgt gctttcttag gcaggtcaat ctcaaccggt acgataaccg 34080 gcgcttcaca accgaaccac tcggcagcat caatggtcag gctgatatcc ttcatcttct 34140 ggtgaatctc gttatccgcg ccggggcggg cgtggtactc ccgtgccatc gccgactttc 34200 ctttctgtac tgaattaaac cagcggtcgg tgaatgcggt gtacgaagaa ccaaggcgct 34260 cacctgcgtc gataaaccag ttctgacccc acaaatcttt taagccgttt ggcgatggtg 34320 tcccggtaag gttaatgaaa cgcttaacct taccgaacgc aaccttgcta agcgcccttg 34380 cccgtttgct cccgcccgaa cggctgcgga acgatttaag cttcgtgctt tcatcggcaa 34440 ctataacggt aaaaggccag tcgtctttgc cgtagtagtc aataagccat tcgataacct 34500 cgtagttagt gcataccacg ttagcgtctg actccagcgc cgcgatgcgt cgcttctccg 34560 aaccagttgc atctactaca ctcagacacg ggaagttcca tttctcttgt tctgccggcc 34620 acgtacctga cgcaacacgt aaaggggcca gaattaacac tcggtcgtcg tcattaagtt 34680 gcccattacg gaacagtcgg ttgaggaccc acaacgttgc ccccgacttg ccgccaccca 34740 taccgcacca tatattgcaa cgtgggtgct gtagcataaa cgacgtcatt attttctggt 34800 attccctgcg cctaaactta ctcatttcgc caccagtaca agttccttcc gcccgaacgc 34860 cgtaacgtta cccgttacat cttcgataac cagtttaccg ttagactcaa caaatactgt 34920 atctacggca acaggtctac gcgtcttaac attgaaaatc atatcgcctg gtacgatgtc 34980 acgtgctggt ttgcggtcat attcatattt catttctcaa ttccttattt ctgtttggtg 35040 ttaactaaat ataatagtgt tctattaatt tatcaacctg tttcttcgac ccaacaacaa 35100 aaacatttgc accgcgttta cgcatcctct cgtgctcgcg caactggtgc gggtctggct 35160 tcgtgttttc gtctttcttc acctcgacga accagatgat gccaccaggg agaatcacca 35220 gcaggtcagg agcgcccact ctattttcgt acgacaattt gcgtacgagg ccccccaggg 35280 cctcaaatcg gtcttttgcg tatttctgga cgcggccctc aggcgtactc atctatgcac 35340 ccctcacgtt tcgtgtgctt aatcccacag cgcggacaga ttcgacagtc ttcttccctg 35400 aaataataaa taatcagttt acttagcata ccgttttaac tccgcacctt ccgctacaag 35460 aggaaaacca tcagcccact ccggcaatgc acacattagt ttttccagtt cagctaccgt 35520 gtaatctgat gtatccggag tctcgcatac cagttcatcg tgaacagaaa gaactattgg 35580 atacccaccg gcttctacat taagcatcgc ataggccaat aagtcacggc acaacgcctg 35640 aacaatgttt tcacaagcct ttccgccgtg tgtgtacagg gtggtccatt gccgtgttaa 35700 ctggttctca ccctggtact taattcttac actggtgttt atccacccgt cttcgtctgt 35760 ttcctttgtc acactaacgc cgattcccgg atacgatagg atacggcctg acggcaactc 35820 catacacaac caccagccag gaaccttcct gcccgacgaa tcaaattcta cggtacgcca 35880 tatgcggata gccctttcac cattccggcg caagtgtgcc ccagcccaaa attcacgacc 35940 aggattacga acagcggcta aaattccgtc tttaaggtcg cgccagaacg ctactgtttc 36000 agggtgtgac tcacgccaca tacgcttgat cgcgtcacat gtgcgccata ctttcttgtc 36060 aagtatatac gagggtcgat catccttttc accgggatgc ggtggtctct tggcttcctg 36120 aatacgcgcc cactcatacc cacgtgcagt agcagcccag atgtggtcgg ggaaagtccc 36180 atccattgtt tttgccatct caataaggtc aagacccagg tttttagcaa acgtaacgaa 36240 cgctccgaca cctccctcgt agccgaggcc cagttcgcag ttatgcgcca caagaaagcc 36300 cgagtctgag gctataagaa aacggttgcc gtcttccaca tcaatcaggt cgtaaactgg 36360 ctcgcagtag gttaatttcc cgttcaagct cagcaatgcg ggcaacggct gcatcatact 36420 ttcgttgaag ctcaggtatt cgacgtctgt tacggcagtt ttcagaacgg ctgacaaacc 36480 gaatgttgcc aggctcgtag ttaccgtctg tgtcgattcg gtccatttcg aggtttgggt 36540 catcccagcc ttccaaagtt tgaacatatc taagaaacga ccttttatcg gccctccact 36600 ggtcaaatac cgtgataccc ctagccccat agtttttgta actcttgttt ccggggcttg 36660 ttgtccgcac gattgccgcg gacaaccggt tgagtaatcg cgttctgtgc gcatcatccg 36720 gcattgcgtc ttcgtagcag aaatactgtt tacgatattt ttttgttgct tcaagggcgc 36780 agcggttgca ttgagtggtt ttacccgctc ggacattgtc ataatagacc cgcccaggca 36840 tctctccaca atcacattgg acgagtagtt gtctttggcc ttgtcgtttt ggtaactcgc 36900 gcaggataac caggcgtcca tacctatctc cagggcgcgg tccatcatat atttgtttga 36960 aacgagttgc tttgcctgct tccaggaatg cgtcaagatt ttgtggtctt cggtcattag 37020 caccccgtcc acatttatca ccggtttcca ccccataagg tgcgcgcctt ttgtattcac 37080 ccatttttca ccactccata ctttatctgt cgatgtgacc gccataatat ccttaaatcc 37140 gccattggtc aaaacttggg tatggcggtg aaggcaggct ttaccaatct ggcgtaaatc 37200 tttacgttct ttcttaatgt aatctggatc catgccaaac atcttacctg cggttacgca 37260 gtaaatatcc agcccggcgc ggaacgtatc aagcgcggtt tcttcgcccg ccaaccacgc 37320 aagcccgcgg ccttcgacgt tagagtaatc ggcaacgaca aacttatgcc ctgcttccgg 37380 aatgatgcag ctacggacgg tagacgctgt tagttttgcc acgtcaaaac ggcggtgtgc 37440 acggccttta agtaacgcgg caatacccct atccagttca tcgtcgtgat aatacccgcg 37500 cgccaggttt tgcggctgaa aacctttccc cgcccaccgc aatgttcgct tcgctcctcc 37560 gtattgcagg caaccacggc ggcggtcatc tgaagaacgg cctaacagca acggcgcgta 37620 tttcgtcgat gcagtagaag cagccccgag acgcatctcg ataatagtgc gggcgtcgtc 37680 cggtaaatcc tcatccgcca gcaaatcgtt tagtgttgac ttctgtgcgt tgtgaatgcg 37740 gtgcgccggc gccagttcct gtaaaatagg caagaagtct ttgccggtta gtgaaccgcc 37800 gtatttgcgt tgggcttctt cctgtagctc ttccttgtgg cgcgtcactg cttcaatcgc 37860 ggcctcagcg agggcaacgt caaccttaaa tccgcggtcg ttaattactt ggtctaacca 37920 cagcacgcgg tcttcgaact cagaattccc ccacttcggc attttcttat acacttcacg 37980 catagacgta atgtcgcttt ttgcgtatgc gataaattct gcccactctt ttgggtgggt 38040 attggcggta tatcgtcgaa tcttatagtt cttaggcgta ggcttactga accgctgtat 38100 gagcgccttg ccgcgtttat ccttagccat gcttgcgtca atgtttaaaa cctcacacag 38160 cgcggccagt gaacccggga gggcatgtcg gaacgcgact atcatagtgt ctataatgtt 38220 ttctaccagg agttcgaatc cccagcaatg cttaataact ggtcggtcga acataaggaa 38280 gttttggcct accagtttga ggttgctgtc tggtttttgc agctttagca tcgccctgcg 38340 caaatctctc ggcatatcgc tgccgtcggt ggcgtcccat acttgcacgg ggccttcatc 38400 gaaagcataa gtgcagataa taatttcagt agtcggatgt tcagcgtagg cataggaacc 38460 gacttttttc aaatcggctt ctgagaatgt ttcagtatcg agataaagca gattcattta 38520 tttgaccctt atagaaaagg cgaccgaagc cgccttaatt gaaaagaaaa tacattaacg 38580 gttacgacgg cgttcgcgac gcggtgtttc gtcttcatca tcttccaggt cgtcaacgct 38640 ggcagaaacc gcagaaccac cgaatgcctt tccttcacct gcaaatttga tgccgtttag 38700 ttttgcgcct aaaactttat attgctcgga gaaccaaatt tcgattgata tattagctac 38760 acaaccgcta tatacctgct taccttcaat ctgctcacca tcaatattga agtctggttc 38820 tacctgcttt tcacccttaa ccgaagtcag gattaatggc tgttgcttgt tctttgcctg 38880 gaagtaaaaa ccttccggga agtcttcgaa cgggttatca cgttcggcga tgtcgcgcac 38940 tgcgcattta tccatatgct tcccttcgcc gtagttctgc ttcatccatt tctgggcggc 39000 ggcctctccc agtgcttccg acactacagc gaataccgta tcgcgaagtt cttcaatttg 39060 cgggtgttcc ggagtcagga taaaagtacc gttatacgta cctttagtaa cggaaccgtc 39120 attattttca cggtctttag cgcgttcgaa tacgttcaac catgcagttt gtactttacg 39180 aagattaagt ttgattccca ttttagtttt ctcgcgtttt agagtttatc caggaagctg 39240 ccaggtcagt gattaagaat ataatagagt actattaact tgtcaaccaa attttcgatt 39300 acgccatgct aaatatttca tcctcatttt tgagtctagc atcctgtcag ctaaaatcgc 39360 cgacgtttca ccaactttct ctaacgctct cattagatag tatataaaag ccaccggtat 39420 tagtggggtg gcaagcagcc accacgtcaa tttcttcatt ccaaatcctc ctccgtaata 39480 tttttccaat caggtcgcct gtcatccgcc gatgcaacac atggcacacc cggcttacga 39540 gttacgagtt tagatagctc agcccaaagt tcggggtctt ccttgccgat gatcttttcc 39600 gcttcggtcg gcgatacggg aacagtctta tccagcagat agccgtttac atggttttcc 39660 ctaaatttat ttacagcatc ttcatctttc cacgtgcgat taccaggacg gccctcaacc 39720 agtttgtaac cgggaacttt cttaccggaa tgcaaagcgg ctgccatagc tttttctact 39780 ttgtcgatgt gctggcgcaa caacggcaac ttctcatact cagcgacgag ttgctccggc 39840 gtaagctcca gtgcaaagtc gtcccctctc actggcttct taggtaaatc tgggccatca 39900 ggcagcaact taagaatatc gctcggctga acatcccccc aaacgctcat atagtccgcg 39960 tcgttgcaga acttagctac acgttctacg gcccgccgat atacatccgt atcgtcttcc 40020 agttcttccg ccagtacaga attaacggtt tttgtacgcg cggcacattg ttctgagaaa 40080 cggcaccatt ggcaaccatc aaccgacggc ttgaagtccg atggtttaag gtttttctta 40140 ccacgtcgat acgcgtcaag tgcggctacc gcacgtttct gcgcgaactt agcgaatagt 40200 tcaagacctt caactgagat atcccactcc gacgcgccac cggtgtgcgg ttgaaagata 40260 accagacgaa cagctgttat gttatagcgt ctctttaggc ggcggtaaac accgagagca 40320 tagagcataa gttgcttgtt ttctttcgcc tctactctat ggcgccctgt tttcaggtcg 40380 ccaataacga gcatgtgatc gtcagtgtta gccaattcct gtacggcaac aaggtcagcg 40440 gtcccgaacg tctcaacgcc tgcgtaaccc gggtgcaaca cctccgtaag attgacacgc 40500 atttccagct tggcgtaagc cgctacatct ataatcgctt tacagtagtc ggtgtacttt 40560 cgcacctgct cgaccatgtc atctgtaacc agcaccgcgc ctttcatcgg cttaattagg 40620 gccttaaccg cgcctttacc ttcttccagc acatatgccc ctacatctcg ctctaacggc 40680 aaagaagtgc ctttgatgta ctggttaaga tgaatctcgg caagtgtgtg catcgccgtt 40740 cctaacaccg cggctttacc ggacgtgttg ggtatatctt tttcacaagc cagtgatgca 40800 ctgcacgcca gccacttttt ggccccagac ggcgacagca gggaatgcac atcgttgttg 40860 ccaccgcgtt cttttaattt catccgtcaa tctccatgta aagcggcccg aaggccgcgg 40920 gatagttatt cttcttcttc gaaatacttg ttcttgatcg ctgtcaggcg ttccaggtat 40980 tcggccaggt cttcgtcctt aatcgcggca agcttcatct tcttaccggt gaactctccc 41040 agcagttcat tggaatcatc gtaagcgtca tcgctaggac cttcgctaat cgcatcgtcg 41100 atagcctgaa tctggtcgcg aagagactgg tagtcaactc cttctttctc ttcttccggc 41160 tcctctactt tagctttacg cgtcttacgt ttcggcttct cttcttccgc cggtttagcg 41220 tcaacgacgc tttcgccttc taccgggagt tctttttctg atgctttgcg taccgcagaa 41280 ctagccttat aattatcctg catcgccatt tctaccgctt tttctgtcaa ctctttgttt 41340 accgtggtta tatcactttt aagttggtac acagtctgtt tcgcgctatt cgcagcaacg 41400 aaacgttcaa gtaataccaa gaatttctct aacattattt gttctcctgt ttagttggtg 41460 ataagaacta taatcgtact ctattattta tgcaagcact ttttcgtaaa ttgcgtcgtg 41520 tattttatta ctgtactatt actacatatc taactaagga gtaaacat 41568 <210> 2 <211> 41515 <212> DNA <213> Unknown <220> <223> Bacteriophage TS6 <400> 2 catgataatt aaaatgtaca ttgtctctat ctccgttggt taactgataa tcatattagc 60 tgagtattat cgtgatgtca acgcaatttc ttcaaccaca tcggattcgg tcggtagagg 120 cacgggcggc gtgcaaactg acgccacacc cgcaccacgt aacgctcgct gccgttgagc 180 tggtgaacct cgacgattac cccggtatgc gtcttaccgc tgtgtgggtg cgtccgggtg 240 acgatatctt ttagtgtgaa catttcgact tggcctggta gttgttgccg attagttcga 300 aatcgttgcg ccagataact ttgaacgaat gcgcgccgtc tctagctttt aacgaataag 360 gcccatctac taaataaaca ttacctgtag atttacacct aaacaccgaa cctttcttga 420 acatggtttc acccccggat agctttcagg atgttcgtaa gcatctccgg cgtcgtagcc 480 ttgatgaact caccggagca taacgtcacg gcgtaacgat gggcacggaa gtctatgcgc 540 tccagttcgt ggttcttcag ttcttctaac aggtcaatca ttttcacacc tctgcaatag 600 gatttatctt agttacacgc gcgtcggggt tgagaacttg tatggcagtc aggcatcacg 660 ccttctcggt cagacgcgat aaatacgcat caagtttctt tttgagtgct gctagctcct 720 ccgcaaactt ctcggctttc ttgtggctgc ctatctcata ttccatcgtt acgccaacgg 780 aacactcttc aacttcggtt atcaattttc ttaagtggct agcctggcgt ttagtaatca 840 tctttccatc tcctctgttg gtgtacacag atattacgac actaatacta tgattgcaag 900 tagcagccaa cattattctt tgcgctgccg gttgatacct ccatctttgt tgccgtgttg 960 catagcgcat aatgaataag gcgacgtgag ggatgatggt ggggaaaacc cacccatctc 1020 tcctctaagg agcagccccc ttaattctcc ccctcggcaa caaacccagg cgtgacgggc 1080 gggggagacg ggggaagacc tctccccctc ggcaatttct cccccaaatc tccccccgaa 1140 taactattca ctttttctga ataactattc acagccaaca ttctccccct tcctccccta 1200 gagcttagtt ttcgaggtca tcttccactg atgttggctg cgaatcttct aaatcgttaa 1260 tgtctgtcgg tatgcggtat ttaacgaaag tgccatcctc accgttaagc agccgtccgt 1320 tttcgtcgcg cccataccag attattttac ggcgggctaa gtcctttaga atcttgtagc 1380 ggtcgccacc cttgccaact atcttgatga catctgcggg ggtgaaccct tcgccgtcgt 1440 cgcgttcgtt ttgcaactcc tccagcgcag cgtagacagc ctgctcggtt ccgcgaagca 1500 tcggtccgtc ctgtctggtc tgcttcaccg cctggcgacc aacatcggca gctttcgagt 1560 cgaacggcag aagtgcgaca gggacgagga ataacgtctc gtcgcgcggt tgtgagtcca 1620 gcggcttgac ggtctgtcct tccaggaatg aactgaagtc aggagcttca cggttagatt 1680 gcagcgccag tatagcgtcg gcgtggcctt gtgcgacttc aatcttgcac ttcgccagca 1740 agaagccacg tggtaactgc ttggtgttgc ctcgggcctt ctccatatag aagttcaggt 1800 gtagcggttc cgactcgtcc ggctgctcga tgaagtatac gaagtccaca gcaccgtgca 1860 gcgcgccgga accgcgaccg atacgacggg tacctttctc cgatttagcc gggtggtgga 1920 ctaccccagc gcaagcccca gtttcatcgg caatcgcctt gagtgccgac gctaccgtac 1980 ccatatcgga agaactgttc tcgtcgaacg gcttatcaga cagcgcaaca gactggttaa 2040 gggagtcgaa cgccaccagg ccgaccgggt ccgttcctgc cgtcgtgcgg atgtggcgga 2100 taatggctcg ctggcctgcc ttgctggtta tgtcccagcc ttcggattgc aggtcgatga 2160 tgtgcagcca gtccaggtcg tcgccgtagg tcttctggag cgcctgcttg cggtccagcg 2220 tcgtgctgcc accttcggca tcgaaataga agctgtgcga tttgataacc ttcgcgcccg 2280 caaacggaat gcccgccgac gcgcacgcca tctggccgag caggaagaac gatttaccga 2340 tgttggattc accgaccagc tcaaacgtgg atttgaagtt gtacaggccg tcgataatcg 2400 ggtcgcgctt ggtgaacagc gcgcacggct cgtccatcag gtcttcgtcc tggcatacgt 2460 gcgcgtccag accgtcagcc gccgcctcta cctggttgcg gtggacgtgc tctacctcgt 2520 cttcgtccat gaacgggaac aactcggaaa tctgcttctt cgaaatgtta tgcgcttcag 2580 gtaaataact atttggcaca ccggcaagct gtagcatcat gtgctggtgg ctgttaagat 2640 tgcgacagtg gtcgtgctgg cacataaagc gcacttcagg gtgtagcgaa tccggaagca 2700 ggatagctgt cgacccgtcc gtgccatcgt taccggtgtg gctcatgtgg ttcggacact 2760 ggatggccca gccacgcccg gacgacatca tttccaggcc catttcgaac gcccagcccg 2820 cgatagcctg gctgttctcc gaagcggcgg cgagagactc ttcagaccat accgtgttgc 2880 cggactcgct cggcggctca taaccattgt tcagcatctg gcttacgcgc accggcttac 2940 ctttgccttc ccaccactcc gcgccatacg gcggtacgaa catgatgcgg gcgcgctgac 3000 aggcagtcat atcaacacct tccatctcgt ccagaccaat ccagttcagg aagctgtgct 3060 gaacgtgcca gatttcgtcc gcatccatcg ggcggttggt cggaataagg aaacgcaccg 3120 cgcgcacgtc ggcgccgcct ttaagtgcgt ggcggtcgga gctggtgccg tgcgcgaaat 3180 aggcgtagcc tttctcatcc agcgtgcggg tgactttgcg cacctggcgg gtggtcacgc 3240 cgtcgaggtc gatgaacagg atagagcggc cgagcacggt gtcgttagag cgcaccttat 3300 caacggcagc ggcgatgtag ttctgctgtt tcttcttgcg gtcgtactgc tctttggttt 3360 catcgcccga gaactcaacg cccaggcttg gctcgtcgat gtagtccatc atctcggcga 3420 tgaactccgg ccaggtcata tcgtagtttt tggcgcgacg ggatttcgca tcgttgccga 3480 tggagaagag aatgttttcc atgttgcgtt acctttattt gactaatcgc gcaacacgta 3540 gctataatcg tgttgcacac actgaggttt tgttgtaatg gttcgaccca ctcttcgaga 3600 aaagcccgca ctgtaattgg tggcgggctt ctttttaacc taacttatac agcggaacta 3660 tgtcgttcca ggcggattta tcgccgtgct cttttgcgct gtcttcatcg gtaaacgctg 3720 taattttgca gcgcacgtct aaactacgct ggcttgtcca ggcgacaggc ggctgagaaa 3780 gcaaatcaac cagttgacac attgtgtctt cgtctaactc aaaagcacca taaacatttt 3840 gttcggcatc tttcagccgt tcaagcagag acttcattcc gcatctcctt ccgccagcca 3900 gcgcgggtca acttccagca cgtcggccat cttgaacagc aggttaacat cagcaatatt 3960 gttacgcccg cagcacaggt ggctgattgc cgattgtgtc acgccgcagc gctgcgccat 4020 ctcaatttgc ataatacctt tcgcgcgcat ggtttcacga aggcgggtac taaactcggt 4080 cggttgtggc ttcattacat agctccttag ttgtttacag ggtgagtata ctctactaat 4140 agtgtaacgc acaagattaa aaatatttta ctaaagtgct tgcataacgg attagtttag 4200 tattatagtt ctcatcacca actgatacgg agacgtaaaa atggcacaat atgaaatacc 4260 ggattgtaac gaccctgaat ggcaatcaca gatgctgcgc gaactggaag aaaatctgca 4320 attacttcgt gacgatattg gcgatgataa ccctaacttg gttgattcgt tatcaatagt 4380 cgatgcgctc cgccagtatt ctggctacta atctcaaacg gcaacgccgg ggccacaatc 4440 cggcatataa aaggaacaaa gcaatgttag ataaatttct cgtactgtta gaacgtttcg 4500 ttgtagctca cgaactgatt gcagcgaact ccgcgcgacg cactgtcact aacaccgctg 4560 gtgatgtatc ggtgacaatt accgaagcta ttaaagaagg cgctatcgcc gatgcgatta 4620 aaaccacagc cgacgtagcg cgccttaaac aggagccaga agacgacctg gacgacgaag 4680 aaccggaagg gaagccgaag cgcaagcctc gccaggccaa accgaaagac gagccgaaag 4740 gcccggacgt agcgaagctg cgcgaagaga ttagaaccat agcaaaggcc atcgctaccg 4800 gtgattctga cgaatgcgcc gacaaattcg acgagttgct tgaggactac aaagtccgca 4860 cagtgaataa gcttgcggat gaagatgtcg aagcgttcca caaagacgct actaagctgg 4920 tcggcaaata ttatgagatt gaggagtaag aatatgtcta tcgaattagg aatgacacac 4980 aaagtatctt acgaagattt ttacgcagag tacagcgctg cacatgacgc tctagaaaat 5040 aatctggcta aatcttattt tatccacgct gtgtttggca tgttgcgccg cagttccgaa 5100 caaagtaacg acctcgaagt gtgttacaac agcgcgaaaa gtgaagacca gaaactggcc 5160 gctattttgg tgaataacgc cagcatcttt aatgataccg gctggcctga cgttacagac 5220 gaagactaac agtcgcgccc tggttcgcca gggcctttta tcaagagggt tagagctatg 5280 aagaaactta tagttaccat agaaactgcg tggattggcg ccgcggaaac agtagaagtt 5340 gaactcgaag gcgacgaaac agaggaagaa attgaagcaa tcgccaaaga ggaatttcag 5400 aattactgca attacggctg gagaatagaa aaatgaaata cgaactttat agcgctatcg 5460 atacccgcga caacaagccg atgtactggt tactggccgg tgtctacccg gaacgcaagc 5520 tggcgctgtt cacaccaaaa actatggctg ccgatgtcaa gcgtaagaca gccgcagcgc 5580 cggactcaat catctgggag tctaccaaag cctggtacgc gcacgcagcg ttagaaggtg 5640 cgaagttgat ttattcatgg gagtttcggc aatgagttac tatttctggt tggctgaatc 5700 tagtaatgga aaccgtattt ctggtgttat ttattccgaa tccccagaag ctgcacatgc 5760 agaagtgacc gaatcggtta ataagaactc atggcataaa tgtacagtga ttaaactgga 5820 gcgcatcgat tgaaacttaa agctaaagta gagaattcca atcaaactca cgcgctgcta 5880 tcccccagcg gcagcgctaa atggttaggc tgtgccgcct cgttaatttg tgagtatgac 5940 attccaaata cttctggtgc tgcggcggta aatggcacgt gtatgcactc gctggctgag 6000 actgtcctta cccgcgtcat caagggtgag cctaaaatta ccgcagacac gtataaaggc 6060 gtgtacgcac tgaacgaagg aaaaggccct atcaaagcgc tggttaagcc ggagaaaggc 6120 gcggtattaa ttactgatga cttcgtgtcg caggtcaata agtacgtcga ctactgccgt 6180 cctattatcg acgctgccga actgatagag gtggagtcgc gcgttaactt gacacgtgtt 6240 ctgcatccag gcgttgaact aaacggcaac cagctacaga ccttcggcac cgcggacttg 6300 gtagcggtca tgccggacgg tatgcttatc gtcggcgatc tgaagaccgg gcggcgtaag 6360 gtgctggcga aagaaaacaa gcagatgatg ctctatgcgc tcggcctgct gcgtgcctat 6420 aagcgcctgt atgacatcac ggcggtgcgt ctggtcatct tccagccgta tgcaggcggt 6480 gccgacgagt gggatacaac accagaagcg ctggagcagt tcggcaagtt tgcacagggc 6540 cgagcgctta aggctatcga cgcgttccag cgcggtaaga aaggtcttaa accagcagac 6600 ttccgtccgg gtaacgtaca atgcccgggg tgtcggttct ccgagaagtg caacgccaag 6660 cgaaaggtag cgtcgcggga ggttgaatca gaccttgagg acgatacgca gcaatcggcg 6720 gaggaagccc ttaaagccat ccgtaaattc tgtaatgacg cggactatat ggacgtgtgg 6780 gacgatgtat cccccgctga tattttgcgg ttaatccctg attttgatgg tcgcccggct 6840 aagacggtaa aagctaagac tgacccatct gaaatatcgc tagaccagct caaggccgag 6900 tgggacaagc tgccgttgat gcgccaacac atcgccgaca tagagaaagc catgtacgcg 6960 gcgctgatgc gcggcgaggt ggttgatggg cttaaactgg tcgaagggcg tcctggtagc 7020 cggagctgga gcgatgaaga agcagttaat cagttccgtg ctaatcatcc taatggttat 7080 ctgttggata aaaccgtgcc tgtcaatccg acagaggccg aaaagattat aggcaaagaa 7140 gaccctgaac tttgggcgga actgtctaaa ctagtaacgc gcaagccagg ccggccgtcc 7200 atcgcaacgt ctgacgacaa gcgaccggcg tataccccgg taaccgatga cgatttagca 7260 aattaattgt tgacactgaa ttattaaact attatattaa gcatcactgg ccgggcagtg 7320 taacccgagc gaaacattaa ctgagaaaga ggatagaaaa ttgggaatta aactgaacct 7380 gaaacgagtc agaattggtt gggttaatgt gtttgagaaa gctgctgatt ctgtaaacgc 7440 taacggtcaa ccggttaaag gtaaattcca gctcaccgca tatctggata agaatgaccc 7500 gcagatttca aagctggatt ctacagtact ggaagtgcta acagaaggta tgaagtcctc 7560 taaggctgcc gagaagtgga tggataagaa ttacggtttc ggcaaccacg cagataaatg 7620 cgccatccgt gacctggcgg agcgcgataa accaatcgaa ggtctggaag aaggcctgta 7680 ctttaaagct acaagtcaga aacgcccggt tattatgacg tctgccggtg aacgccaggg 7740 ttctgagcgt ggcctgaccg tcgacggaga cgacatcgaa ggcaaagagg tttacgcggg 7800 ctgctacgcc aacatttccg ttgaaatcta ttggtacgac gcgtataaaa cgctgctggt 7860 taatctgctg ggtgtgcgtt tccgtgaaga cggtgaagcc ttcggcggtg ctggtgaagt 7920 agcgaccgat agcgacctgg acgatgacga cgatacgccg cacaagacca agcgccgtcc 7980 aagtcgcgat gaagacgacg aagaggaaga acgtccgcgt aaaaaacgtc gcagctatga 8040 agacgacgaa gaataaggac atttgataac gacaaggccc gctgatgcgg gcttttcttt 8100 cagtggagca aaataatgga actactttat ctcgacctcg aaacattctc cgaagctgac 8160 ctgaaaaagg ttggctcata cgcgtatgcg gaacatcctt caaccgaaat cctgctggct 8220 atttacgcca tagacgacgg cgaagtacat tgttgggact gcacggcaga tgacaagatg 8280 ccagatgacc ttaaacgggc cttacgacag gtgcagcgac acaaggctaa gatagttggt 8340 caaaactttc tcatgttcga cagactggtt attaaaaacg tatgggggat agaacttgac 8400 ccgcgcgata taattgacac gatgatttgc gcgttccgcc attcactccc cggcagcctt 8460 gctgcgctat gtgaagtgct gcaaatagat gaagacctgg cgaaagacaa gagaggcaaa 8520 gcattgatta accgtttctc taagcctacg ccgaagaact ataagattcg tcgctacgac 8580 cgcatcactc acccggaaga gtggaaagca tttatcaaat acggcgtctc cgatatcaca 8640 tccatgcgcg aggtttacca ctcgctgccg cagtggggta acaccgagtt cgaaaatacc 8700 gtcctggccg tcgaccagcg catcaacgac cgtggtttct acgtcgacac ggcgctggct 8760 aacgcggcaa tcgaagcggt gaagcaacac aaggaccaat tgcagtggga cgcccgcgca 8820 aagtttggcg ggaaattaac gggaaaggac tttctcccta tcttgcgcga cctggcacct 8880 ggacatgaga ttctcaacgc gcagaagtca acattacatg accttctggc cgacgatgac 8940 ctgccagacg acgctcgtac gattattgaa atgcgacttg gggccagctc cacggcatcg 9000 accaagtaca atccgctact gctgggcctg tccaccgatg gtcgccgtcg cggttgtatc 9060 cagtacggcg gcgcgtccag aactttacgt tttgccggta aaggctttca gccgcagaac 9120 ttagctcgtg gttattacga tgatgacccg gaagataaga ttaagccgtt gcctggggag 9180 acatataaaa attaccgcct acgtaaactt gcgctatcaa caggtataga catgctgttg 9240 aaaggccgag cgcactgggc ctatgacgtg tcgaaactaa cagctactac ggttcgtagc 9300 tgcgttacac ccacccccgg caacaaactg ttggttgccg attattccaa cgtggaaggg 9360 cgcggtcttg cttggttggc cggtgagaag acagctcttg cgacattcga agccggtctg 9420 gatatctatt gcgagacagc tggtaagatg tttggtatgg aaccggacta tattaagaaa 9480 aaccgtaagg atatcagaca gataggtaaa gcctgtgaac taggtctcgg ctacggcggc 9540 ggcgtcgcgg cgttccttac ctttgccaag aacctcggcc tggacctgta cgcgatggcg 9600 gagacgatga aaggcacatt cccggaacat atctgggccg cggccaagag aggctatgag 9660 tatgcgcgga ttcaggagaa gaacaagaag ggttttaacg gtcagaaggc ggagcgcccg 9720 gcttacgacc tgccgaagaa tgtatggctt acctgcgata gcatcaagcg catgtggcgc 9780 gaatctcacc cggcaacctg ccagttctgg actgacctcg aatccgcagc gatgaacgct 9840 atcaaagacc ccggtacagc ttactgggcc ggcgcggctg tgcgtgagaa cggcgatagg 9900 gcgattaaga ttacccgcac gttcaccaaa gagaaaggcg aacgcgtgcc cgggtggtgg 9960 cttaaagtcg agctgccgag tggccgtgtg ctgagctatc caggtattgg tatttctgtt 10020 gaaaagcaga ttgacgagga cgacgaccgc acagagtacc gcgagcgtat ccgctacatg 10080 ggccagaacc agactacacg gcaatggtca aaacagtaca cgtacggcgg caagttgtcg 10140 gagaacgtaa cgcaggcact ttgtcgtgac ctgctggcgt atgcgctggt caacgtcgaa 10200 cttaaccagg ggtggcctat cgtacttcat gtccatgacg aaatagtgtg cgatgtacca 10260 aaaacagacg aatattctat acaagggctt ataagcaaaa tgtgcgagct gccgtcctgg 10320 gctgctggct tccctcttgc ggcgaccggt gatgaactga tgcgctatgc caaataatat 10380 ttgactaata attagcgatg gcttatcttt aagtcatcgc ttaaacacat ctggagacag 10440 ccaacatgaa acacgttaaa ttcagagtcc ctatctaccg cgccgacgtt tgggtagtaa 10500 ttgatgaaga agaggcgtcg cgcctggcct cggtgaaatg cggagtcttt aacgacgatt 10560 tcaacatgtg cggcgcagta ttcttcggca acgacaataa cgtcgtatgg ctgccgtctg 10620 attgcactat gcggactatg gcgcacgaag cgatgcacgt cgtgctgaac atctgtcatc 10680 gtcgcggcgt aattgtcgac actaacaacc aggagccggt tacctatctg actggccata 10740 tctttagcga gattcttcgt gcgcacaata aactgaagga gcgccgccat gacgcctgaa 10800 gggaaaatac aagcctatgg catggctgaa ctgaagaagc gtggttgcct ggttcgcaag 10860 attggctacg aaggccgccg cggatgccct gaccatctgg tgctggtgcc ggagcgcact 10920 attacgtggc gagatatacc gcaaggtcgt agtcccgcgc aaatcgtatt catcgaatac 10980 aaagcaaccg aagacacgag gcccgaagaa caccagctac gggaacatac ccgtatgcgt 11040 gcggtcggtg cggacgtgag agttatcggc agcaagcgcc agattgacga gctggttaag 11100 gagttattcc cgtgcgacta acctacctgt gtgaacactg tgggcgtaaa ggcttaaagc 11160 ccttcggctt ccgctgtgaa gctgtctggc gtttacggcc ttattggttt tgcagcagac 11220 aatgcgccaa aaaggaatat gagaaatgcg actaaccccc aaagaacccg agccgcgtaa 11280 gcgctgtatc cagtgccgcg agcgtctacc actgtcgtcg ttccataagt ccagtgtagc 11340 cagtatcgac ggctaccgga acatttgtaa aagttgccgt cgtgctacag aagcggcgcg 11400 tattcgtgag aagagaaaca aatgaaatgc tttgatgacg accgattaga ctatgaccct 11460 gaaactggca tttttatatg gcttgtcgca ccctcgggtc gttctgtcgg cgctatcgcg 11520 ggcgggagta aaagcaacgg ctacgtgcgc ataaaactgg atggcaaaat gcaccaggcg 11580 caccgtcttg cgtggaacac tcttcaccct aacgatttaa tcagcccaga tgaagagata 11640 gaccgcatca accacaaccg gaccgacaat cgcggtgtta atttacgtaa ggtctctaaa 11700 gcggagaaca gtaaaaatca atctctttat aaagcaaata aatatggcat aactggcgtt 11760 ggatttatgc ctgaatttaa tctatggcgc gccagaatcg gcgtcaatag agaactgatt 11820 gagcggcatt ttcgtcgctt tgaggacgct tgcgcgcaac gaatagaatg ggaagttttc 11880 tataagttcc acaaaaatca cggtggcaga tgatggctaa tttcgttcga agagattatc 11940 agaagttaat cacggcgcac atcatgaagc acccccgttg taatgttttt gcctcgatgg 12000 gtagtggcaa aactagcagc gtattatggt cgcttaacaa gatgtaccaa tcgggagagt 12060 tagaagactg ggaccaggag aactggtctg gcgacagagt tcttgtttta gctccgcttc 12120 gtgttgcatc aggaacttgg ccaaaggagc agacgcagtg gcagttcccg gcgctgcgcg 12180 tcgtcgacgg caccggcagc cgccagtatc gcgaagacgt gatgctgaac gacgatgcca 12240 acgtggtgtg ctgcaactac gacatcctgg aatggctcgt cgaattctgg ggagaccgct 12300 ggccgttcac ggtcattgtt gccgatgagt cgaccaagct gaagtcgttc cgcagtcgcg 12360 gcggtagtaa gcgcgcccgg gcgctcggca aggtcgcgca caagaagatt aagcgcttca 12420 ttaacctgac cggtacgccc gcacctaacg gcctgaaaga cctgtggggc cagtgctggt 12480 tcctggacgc cggccagcgc ctcggcagca gctaccaggc atttaccgat cgctggtttg 12540 tctctattca ggaaggaagt catcatgcca ctaaatcgta taagcctcgc cctggcgctg 12600 ataccgagat tcaccagaag attgccgaca tcagccttac tgtggacgcc gccgaatact 12660 ttgggtgcga caagcccgtg gtggtaccca tcgtggttcc gctgccgtct aaggctcgca 12720 aagtctatga ccagatggag aaggaactat ttgcccagct cgaagccgga gaggtggaag 12780 cggcgaacgc ggcagcgcgc acccagaagt gtctccagat agcaagcggc gccgtgtaca 12840 cgacgggtga agacggcgaa gcaagccgtg aatgggagcc ggtgcataat gccaaactgg 12900 acgcgctgga gtcgatttac gaggaactga atggtgcgcc gttgcttgtg gcgtaccagt 12960 atcagcacga ccgagcccgc atcctgaaga agttcccgga tgccgttgcg ctggcgaaag 13020 gccagaaggg caaccgacag attgaagcgt ggaaccgcgg tgagattcct atgttgctgg 13080 tacaccccgc gtccgccggc cacggcctca atcttcagga cggcggctgt catctggcct 13140 tctactcgat gacgtggaac tatgagcact atgcgcaggt tatcgaacgt atcggtcctg 13200 tccgacagca tcaggcgggc cacccacgcc cggtgttcgt gtaccagatt caagctgccg 13260 atacgctcga ccaggttgtc caggcgcgcg tagagggtaa ggaagaggtt caggatttat 13320 taatgcagta ttgcaaacag aagagaggtt aagaatgaaa aagtatcaca tgggcgaagc 13380 gatggatatt ctgaatagct cagaaaaaga atttattggt agaaaagttg aaaattcgca 13440 tttaggttgt atgggtgcgg tttgtgttga aacttatcgg cgggctgatg gagttattat 13500 gcgtatgcga acatcaaatg gcggttgtac gctggagatt gaagaatgac gaagcaagaa 13560 atgattgaat gtctgcgtga gctaatcaag gaactcgaag ccgagaacgc cagactacgg 13620 cagcagcggg acgcggcgaa cgcgcaatta gactggctac ttgaacaacc ggaattaagt 13680 aatgatttgg ttgcaaaaat acaggagagt taagtgaaat actcaattgg cgcaaaatgt 13740 ttcatcgcag gacttattat tgccggggta gctaccgcgt atgcccttta tctggcagta 13800 tgaaaaaggc cccgaaaggg gcctttactt tttatgccaa agtcaatctt gtatagctgc 13860 cgtctgcgcg cttaactaat gctttaagac tatcgccttc taggtatagt gcgatgctgc 13920 cattatctct aacggcagca tcgggtagca cggtaacggc tactggtatt tctaccgcgt 13980 gatagtcgcc gcggttaatt tttagtgata ccgcatccgg taatgaccca gaaatagccg 14040 taatttcagt ccaggcggaa ccagaaccgg ggccaccatt cagcgtagag cgaatagcac 14100 cactatccaa ggttttggta agcttatgga ctcttaaacc catttccacc gagtcaccat 14160 taaacccatt aatccttgtc tgccctaacg cagaatcaat aatgttacca gcaagaatac 14220 ggtctggtgg cacgttacct acaacaccac ttagggtcga gttaggtgcg ctgatgacca 14280 tacctttgga tggctgaggc ttaatgcctg cggctcggat accatttaca atacagtcac 14340 caatgatgat tatctggtct gaatcaaagt tcatatagtt gcagtcaatc accgtaatgt 14400 tagagaaggt acggttgaat gtgtgagcaa gcataccagc gcccgcacaa tcctgcacgg 14460 taacattcga cacataacca tcacgaccat ccatgcctaa acctacacct aatgagttca 14520 taacaaggat gttatcaacc atgtggttat taggtagctg atgcatagga tattgagata 14580 caggtagatc tcccggtcta tcgtcttccg gcgccattcc cgggtctgag cctaagtcaa 14640 agccgtccca cactggagat aatgcaactg agtcacggaa ctgaaggtta tagttgcgag 14700 ctgtaccggc acctacagag ccttgccatg ttttaaaccc agactcaccc gcgcgccatg 14760 acgtcacacc aataacccca ccattgtgcg acgcacctcc gttgtttcga aggaactgca 14820 caccactacc tgagccataa tgcacacggc cacctatggc atagttgccg atgccccact 14880 caccacttaa gttttcaaag gtgatgatgc cttctttacc accaatgatg gtgtcagaat 14940 caatcacctt acaatgatga cagttgcgga acaagtaagc agccataagt ccaccagcac 15000 ttctgacctc aatacctaca cactcacgga tatccagcgt ggacgctaca tgctggtctt 15060 ttacattctg tggtatcaat gcctcaagac cagggaactt gacccaatca ttaactcccg 15120 gttgataccc ctcagtctta gattgtttaa gtgtagcagc gacagcttgt gcatctgtaa 15180 tccacttccc atcttctgtc caggggtaaa taacccacgg ggtcgtagcg ctctccatga 15240 aaggcttttc tattatagaa ccactaccca tattagtgaa aatcaaagcc ccatcgccga 15300 taaacttcgc tttacagtcg atggttatgg tcttaccgcc gaagttaaca gtctccccat 15360 tggtaaaggc gtagtcacgg tcgataagaa cgctgtcgac ggcggcatcc gccaattgtt 15420 gtaacgtagt aaagtcggac agcttaacgg agtatttaaa tttcttatct gcttcggcac 15480 ggaaggtaat atctccgaac gcgtcccagg cggaatccga aatgccgccg gtcgcaagag 15540 gtgtcgacgc cgcagggata actttcggca gggagccacg ccatgcgtag tagttaccgt 15600 ctccaccatc ttctttcggc caaagaacag ctttatctgc gtcgtttacg ccgagagtac 15660 caccggtcgt aaaattaaaa gatgccgggg agaaaccagc accacgtaag actgcgggaa 15720 gcgtcttctg cgtctgcccg gttacctggt tagtggcgta gtcgatatct gcaccgccgg 15780 ctacaccgcc ttgtttaccg gtgataacct cggcttcgaa aatctggtgt tttttggcta 15840 tctgtaaatc attaagtgac attacaccac cgcaaccgct ggacatatgg ctatcctctt 15900 agttaaaacc gttatcgaat ccgttagaga acgcacgacc gaaagggggc acgttgtcaa 15960 acttatagaa atccttgtca tagttaaacc ctgttatttt aacggttcgg tcatcaccag 16020 ggtctaccgt agaaacaaga atcatctgag cattatgcct tgcttcgttg ccaaatgaaa 16080 actcagtttt taaagcgctg ttgccggtgt atattgcctc ttgaggtact tccgccataa 16140 tcacctgcct gtcgtgagac ccgggggtga ctttgacgct ttgaacgcca ccgtctcgcc 16200 gcttgagaac aagagagtgg tcgtctcctg acgtaaattt aacaggctgt gacagctcaa 16260 cagttaggcc gtttactgct gttatatagc cgtcataggt agccatccgg gagcctttca 16320 ctacactaat agccctgttt ggtagcgcaa ataccccctc ttccgtggca ctaaacgaga 16380 cggaaatctt cttaagaatg tttttctggt gtcttctgtt tgcggcccag aacgcctgct 16440 tatagttgcg gattcctttt gagtcgtaag tctccgtttt aaggcccccg gtttcgggta 16500 tggttatagt ctctttagtg ttagttttcg ggtctatata agagaatttt aaactgtcaa 16560 aaacctgtgc gtcattgaag gttcttgtcc atttttctga ggttcctgcc ttactgcggt 16620 gcgtaaacac catttccggt ccaattcgag gcctctcaaa gtcaagcaga atgtcggcgc 16680 ctctacggta tggcgcgcaa aaaatagcat cggcaatagt gttgacgata tcctgcatcg 16740 ttgttttata atcatcgaag gtgtaacaga attctccggc ttgcttgtcg ccgaagtaat 16800 cctctatttc cttttgtaca gcaagcaact tatccatatt ccttactgtt aaatcgagcc 16860 cgccgacgtc agggtccctt gcgaggcgaa ttaacgattg gactgcctgg ttattaggcg 16920 acatcgtatc ctcaaacact ccattcccaa gatatttata taccatctcg gtcgcaatca 16980 tgcgcaactg aggttgctta acttcggcag cccgcggtgt ctgcttacgc attgagtgca 17040 cagtggttcg gttgccgtag tgcggggtgt tgtcgcgagt ctgcccgtac aggttaacgt 17100 aggttatttc gtctaccact gagccttcga aattgaaatc aaggtctgtt acccttcttg 17160 ccctgacgcg aacacgggac gctactggta aatcggcgta tatagtcacg ccgttatagt 17220 ccgaagtgcg cccggatacg gttccttgcg ccgcgtaaat cgggccgtaa ggtacactat 17280 tttcgtcaag aagttggtat tgtatttctg ctgttaccga agccaacgtt ttggaacgac 17340 cattgtcttt gtacatcccg tttgcagcag ctatgttagc gacaatacgt tccacctcag 17400 ttcggtttat ggacacccaa tcagttaacg ttttttcata ggtgttgtct ggttgcaatg 17460 atgcttcacc atctccccgg aaaaatgaac ctggctgtat ttgttgccat ttacttataa 17520 gattaccggg gactacgaaa gtaacagttc cttcggaaac acttattacc tgatatccgc 17580 caccggaaag gtcaacatct gtatttgtcc aggcggatac gagagaaaac gtatcattcg 17640 gcacaagaac accggagaag tcagaccctc ctgacgggtc gtatatagtg ccggtggaac 17700 cagataacga agggtatccg gacatgtaag aaaacgcgat gccaagtccg ttttgtgctt 17760 tcagaaccac gccgtctact tcatttgatt cgacagtcag atataacccc tgctctatcg 17820 ggtctccgac cagtacctgc ggcgttgccg tgttattcgg tgacgtgtac ggcgcgtaga 17880 cagccactga tgtacctgtg atgtcagaca cacgggtatc accgtcggtt attccgtcag 17940 ggtgtatgtc aagataacct ctaccagcat cgtagtatcc atattctata atgcgacccg 18000 ctgcgttaaa caccttataa gttgacataa ggttgtttgg tatcgtttgc accgtcccgc 18060 aaatgtcata gctacgctca taggggcgcg ctttattatt gcggtctgaa agactgttgt 18120 tgggcgagtc tgcttgcgag ttagcgaggt ttgacgacgc gcctttcact gaaggggaca 18180 aaagcttcgc cagtggtttt aaaactacac tgaaaatctt catgacacct ttaatagctc 18240 cgccacccgc ggactcaaca acatgaaaga ttgcgttttc tttcagtgct tcgaagtctt 18300 ctgttacgtc gttatcttct ccgatttcgt caatgaaaac acgcaccggc accccgtccg 18360 gaacatggtt cacgacgaag ttcatcggat tatcgcgata acgtttaacg tcaaatgtgc 18420 cgtcttcgtt gcgggtatag tggattacta gcgccaaaat tcaatctccg aatatgtatc 18480 tctaaggtct tcaagccggt ctagtctgac ctgtcgtgac gccagttcac aatggcttac 18540 caggccttca tagtatacgc cagcgtgcca cactattcgc ccattatgtt taacccccaa 18600 taaaacggcg tcgaagttct gtggctccgg cacgcgaata agtccctggg ggtctgagtg 18660 tcctgcgtca aaggcctcag atattgccgt ggggctttta acatcgaatt gcggggtggt 18720 aagccccaca tctgacctga cattacgtac atgatgccag cagttacgac tgcgaaagtc 18780 atacggcagc ccggtatagt cgttgatgtt catgatgcca gaattccgcg taaaagtggt 18840 atttcttctg gtgtcataag gattcctgtt gctcgctggt tgagcatagg cgtacccgtt 18900 tccgcggtga acacgccttt ctcttgtgtt aaagtttgta actcgtacac aaccgggccg 18960 tcacacggat atgtcaaatc cgtgctcaca tacctacgaa atataaactt aggtaaaatt 19020 tcgttatcat atgggatgtt atccatttcc tcgtccaata tgttcaatac gtctgggagg 19080 gagaaagacg ctgtctggtc catgtcgtta ttgttagccg cattttttgc ttccattgga 19140 gtgccctcaa aagtgacaac ctctccggtc tccagtgttg ccgtgatatc ctgggcaccg 19200 cggacgagca gccagcgctt acttaacaac ggatggtata tctccagcgt aatgtaatcc 19260 atctcaccat cagggttaga tgctagtttt cggcggtagg ccgcttctac tgattcctgg 19320 ctcattgcat cgggtcccat atacgaggaa atgaagtctg ataggttccg taggccttaa 19380 gaaaaccacc aagacaatca ccatagcagc cgtacaaatc tggcaaattt tcgttaaggc 19440 atgttgacgc tacgtcctgg aacggagatt tctcggcagt tgcggtaaac gttatcaccc 19500 agttaatccc atcatcggta gactcgttcc aggtagatgt tatggtgacc tgatagtctg 19560 caataccttg tcccaggtca tgtttcatcc aaaaactgga agctccgcca tcgactttct 19620 ccataaatga gagaaacgcc tgacgcccca atgcggacac taccagggtt acgctgatgg 19680 ggaatatatc aaaataggtg tcacgcccct ggcgaacacc ccctcctgcc aggtctaccc 19740 gccagatatt gttgccgcgg gtcattgagt accctttaga tactatcggc tttaatgacg 19800 ctgggaaata ataatcactc atatttagta tcctggttgc ccgcgcgttg cgcgacgaga 19860 tttagatata gcactgttac tgtcctgtag tgccgatgat accgtttcac ttattataat 19920 gcgaagtctt ccttcgtcat cgcgttcctg ggaaacggaa tcaaccctac ctgtggtgtt 19980 gtttacaatg gtaatgtttt caccaccaga cttgccgcta ctttccccca taatttgccg 20040 catttgctca gcggtacgca ctcgggacgc cccggctggc ataatgactt ctggcttacc 20100 gcgctcagct atggtcgata tctgtccagc ggccaggctg ccgccctgct cacgcgcaga 20160 acgaatcttt ccgatgttag ccagcccagc agcaacagca gccgcagcgg caacgggagc 20220 caggtaaggc cccacaacag gtatagccgc tgttgatttg taggcctcaa tagcggaggt 20280 gtacgtcgca atcgtagcct gggctattgc gaaggcttta tatgctgttg acgctttgcc 20340 taaggcagtt ccgatgttac cggccatgtc gccaaaagcg tcgccagtag ctttagcgcg 20400 gcttaaagca tactcatcgt tgatagcgga tagcgtctgc tggtatgcat cttccgtgat 20460 aagaccttgt gagtggaaat cgtttagttt ctgtagctta acttcatact gtctgtccag 20520 ctccgcgaat tcactatctg cgagggcttg catctgcgcg acataagcgt cagccgagaa 20580 ctgtttttta atccgttctt ctgtctgtcg ttggagtatt tcgttacgtt tagcatctgc 20640 gtcgagcgca atctgagttt tagcctcttc aaattgctgc tgtgagattg tctcgttatc 20700 ccggaaggcc tggagtttct ccagcttctg ctgttcctgc gcgtcgatag ccgctaactc 20760 gtcctggttc tggcgttgta gagtgtcaag gaaattacgc gctgcattct gctgccgttg 20820 ggcttcagtt tccgctcgct tggcctgctg cgatgcacga gtgtccgcgg ctttctgttc 20880 cgcagccgca cgtttagcct ccgtatcttt tacgcgctga atatcctgac gcgcttcctc 20940 atcgcgtgcg gcgttatacg cggcaatctg ttctttagtt acaccctcac gtttagcaaa 21000 agcctctttg tcggcttgag cttgtgctgc gtaacgttct ttatctgcca ggttgcctat 21060 ctgaacgttc ttaataatct ggtcattctg ttctttaaga cgctgcgtgg tagagtttac 21120 tgatttgccg agtttatcct gggaaccaga aagagcatcg gccttcgacg ctgcatcctg 21180 catctgacct atatttttaa acagttcgcc gttgaactta accagttcgc tattggtgcc 21240 ctgatactta tcgtttagac gagcagtctc gtcggaaaga gccttaatgt tctccggcga 21300 cgcgttcttt tgcaccgagg ccaacaatgg tactagcgct tctgcttcct tattggtgat 21360 accaaacttg tctgacaggt cttcgatgta cccagtaagc gatgacgttt gttgtcctaa 21420 ttctaacgag cgttgtgcgc cgatagccga accgttggtc cagctatcga ggctatcaac 21480 caggtcggta atggcttcct tcgcgccttc tgtttgtgtt acagttgccg attgggcctc 21540 atagaatctg gctagcgctt gggacgcagt acccgtatcg ttcgccagct taacgaaacc 21600 gtctgataat tcatacactc cgtctttgtt cttctggaga acatcctcaa gagtcttcgc 21660 tgacgcttgt aattcttctg cgctaatttt agcaccacca agcgctttat acagtgtccc 21720 ccctacagcg gcagacaatg cgataattgc gccaattacc gcgccgccag gaccgaacgc 21780 cccggctaat tggctgcctt gctgaccgat tgccacaaat gcagaagttc ccccctgaat 21840 ctgtacaatc atatcctgta cctggtagcc tacctgttgc gcgcccgtac cgaagccgcg 21900 tagttttgtt gtctgctttt gggttgtgtc gcctagctta ccggcggctg tgtcggcctt 21960 cccagcggcg ctagcgaagg cgtcaagctg gtcttccgcc tgcgaaacac cttctgtttt 22020 taccctcgca attaacgaag ctgtatcagc catcttcccg accctcaaat atagcgtcta 22080 tacccatgat aagttccgcc tcaagcgcac ttattgtatc cccggtcaca gatttatacg 22140 cgaccaggtc ctgccattta attatatcac gaggacataa taccagtgat tctccggtgt 22200 cgcgctgtat gaattttaga tttctgtact tatcccatac tccgacaaat agcgccggta 22260 attcaggatg ttcctgctct ttcttcgctg ccggttgaat ctgtccgata gctgctaaga 22320 aagcctcatg gcccgctccg atatcgtcaa atttctgttt cttctgacgg tctacataac 22380 tccagcgggc aaaatcatac agcgctttta cttttccatc aaagacttgc ggctgtcgtg 22440 gaaatgtttg gctactactg ttcctagccc cttgtactcg ttaagtagct ttttaagtac 22500 atctttatta aacgtttcat cgaacgacca accggtgaca acggcaatag ctagcgcatc 22560 atttagctcg tctgcgcgcc agttaaactc agcgttatac tgtgaccagt cattcttatc 22620 tttgcatttt tggtcaagat cggataattc ttctttaata gcaaagtatg ctcgatgata 22680 gtctctcccg gctttaacac cgacatcact ggcaggccca acgacgcgaa gccactcccc 22740 ggaatcttca ccactcggca gcgggatagg catcaacgta ccagccgcgt gcttatccgc 22800 aaaaaagaaa tctttcagtt ccatcattta tcctttggtt aagagggtaa cggtttagtt 22860 aaagcggcaa gcagtaacca tctgcctttc aggaatgacc ctagccgcaa aattattcta 22920 tcctttttgt tgcgctttta ctaatcgtcg tttatattag tttcataata tattaacgaa 22980 gaggtttatc atgaaaacat tacttgctgt tctgctctgt tcttcaatgg tccttagctt 23040 ctcggctgag gctgtatctt gtcgcgacat cggcgctgcg gtcgtagacc agtttgttgc 23100 cgttgctcgc ttcaaagtcg aaacaccgga acagattcag ggctacaacg gtctggttgc 23160 gtcgcaggtg cagacctgta tcgacggtaa gaagctgcgt ctgagtggtg tgtcgtccaa 23220 agatgcgggc gtggctatga cgctggcggc agcgcaggcc gtcgaacgtg gcaacagcaa 23280 caaaacgtct ggccttaacg ctatcgctat gacgcagaca tccttcgctt acggttacgc 23340 atttggggag tgattgagat ggttgttgaa ctttgtaaat aaggcccctt tcggggcctt 23400 tctcttatgt gtaggcgata cgctgaatca cgatagaaga ctgcatctga ttacctgtag 23460 cttgaccttc catgctcagt gttaccgatt ccgggccgcc aatctccgga gttgccgatg 23520 ttaattcggc gcgtttcaag gtaaatgaca ttgcaccgtc cggccccgac aggatagacg 23580 tcagttcaat ctgtgtctca ttcaggaaca aattcaacag ggtcatgtcg tacagtttac 23640 ccgccaacga gaaggtgtta gctgcgcgac cacgctcaac gaaggccacg ctatcattac 23700 ccagttcaaa ctgtgcagat gtgccgttgt cgttggtaat agtgaatgtg tcaataagct 23760 tcagcggcgc ggtgccgcta aaagcagaca catccacaga agcaaatggc tgcgcgttga 23820 agttaacagt aaacgttgag ccggctggtg gtgcgctcag gatttcctga ttcagaccga 23880 tgaacgggaa cgacccggta accattgcgt taactgcctg ctcgatagtg aaaccggtga 23940 actctacgcc gcgggtaata agatagctat ctgcaccacc gcagcggcct ttaaaccatg 24000 tcaggataga gtaggtttta cacaagttcc ctgtttccaa cttatcggca ataaccaggt 24060 cagacgtaac agcagactca tctgtcaggg tatggttaat agcacctccg gtaacaacca 24120 gggccgaaac agctgttacg atgaacggct tactgttacc ccctgtaaga ccgggaaagc 24180 gaaccaggtc gccaacctct acggctgtgg tgaaatcccc agcatcgcga gtgaaggtct 24240 tcgcgtctgc gtcgacggtt acagacagtc cggaaacggt agaaccggca acccaggaac 24300 ttgtcattgc accggcaagc aattcatcct ggctttgcgc gctcagttca atagcgtatt 24360 ctccagaaat ctgtttattg cctgtacgga tggatgaggt ttcgcggctg ccgtcaagct 24420 cattagaaac tagtgagtcg cgtgttaatg ccggaatacc gccggtgttg cgcagcggcg 24480 accagctcgg agaagtaggc gtgacgcccg gcgttgtttc cagcacgtag aactgtgcgg 24540 tgttcgcgcc cttatatggt tgcagtgcca tcgttatatc ctcgcagtga aggcaatgaa 24600 attaattgac aacgggcgct tggcccatcc cttttctaca atcagtggcc ccaggctaac 24660 cgactgcact tcggcgcaaa tagcgttacg ctggaacgtg cttcctgctt tgaacgccgt 24720 atttaataag tctgccattt tattgattgg cgcgctacct ttagcggagg ccacgttgat 24780 atcaatctgg taaacaccgg cacgctgttc agtccagaac aagtccgcct gttcggtgtc 24840 ggccagaagc atgtaactcg ccagatacgg cgtagctgtg cttgtgggcg cgtcgatgtt 24900 ctccagcgca acggcaatgt tatttgcgct gccgaaggct ttcaacgcaa tatcgaatac 24960 ttttgtcagg tcttcaaagt aacccattat ctcaccttcg ctgcttcttc attaagcaat 25020 gtgttgaacc ttgctatgtt aacacgaagc ataccggttg gagcttgttt agaccagcca 25080 tactcaagac gttcagcgta cggtaggtta tttgtcagcg taaattcgta ccagtttggt 25140 gcgtttccaa tgtatgtata ggctttagta acggccttat tccctgttgg gtcaacacca 25200 gcaactagcc ccgtcgccgg agtagcgccg gaggtctgcc agttaccacg gaatcgcccg 25260 gtgtcaaccg gactcgactt aattattgcg ctgaatagct tcagtgacac agcgcgcatc 25320 actgtttccg ggttcttctt ggccttcgca acaaaggcct gaatgtccag cgtaaactta 25380 ctcatttgcg cacctgaatg aaccatgcga cgatatcgtc gttaacgatt ttcttctcga 25440 taccaacaac ggaccattgc tcgcctgcaa actcgacctt atcttccatc ttcggcagca 25500 cgctatagtc cgctttcacc accatatccc cagcctgaat agtagttccg ttgacaagac 25560 ctgcgttaac tggtacgggg gcagatgtaa gaggaatctg agtaccaggc tgttgcacgt 25620 attctccgag gtcttcgtcc cacaccttag cccccgcgcg aaccaacgta acagtgctgc 25680 cgtatttacc gagcaggcgc gtcgctacac cctgcatttt cttgctgaaa gcagtactca 25740 ttatactggc tccagtcgtg aaatgacgag tagcgctgat ggtgcgacac cccacgcggc 25800 tacagttgcg gtctgcgggt atacgccacc gaaattagtg ccagcgctat ctctcataat 25860 ctgcacggcc atagtttgcc ctgcgatagc gttaataacg acgcgtgact ctataggtat 25920 cgtaacatcg gcgcttacta gtttagtagc cgcaggtgag ccgtactgag cgccgttcac 25980 cagaatgcgc gacaacagga tagatgtccc gctagcccct gtgcgcccag cctgtaattt 26040 gatgcgaacc gcataattac ccgcggtgtt aaacgtaaca agaccgaccg cgttaatcat 26100 aactgggtta gatgccgcgc cctgagctgc gccaaaagat aactgtaacg cagtgtcaac 26160 tgccgttggt gcttgtgcga cagtagaaga ggccctcagt acttccacct ctttcactcc 26220 ggtggcagca tacaacatag agtctgccag ttgcgttgtt acttctctta gcttttctgg 26280 tgtgatggct ccggtcgtgt tatccggaaa attgcccccg ataagcgcaa acatttcact 26340 cttcgtttta gccatctcta accccgataa acattaaaag agaaagcgtt gttggagcca 26400 cacagcagcg ggcgtaatgc gtccatagat gccgtgatag tgattgtcga cccggtgttg 26460 ccgttgttgg tgtactgtac tgtcaccgca ccttctacgc gctccatagt cgtgacgcgc 26520 ccgtctgaac tggcgcgcac atccgcaccg ttaccgtact ctacgccagc gataagctgc 26580 gcgagtttaa cctggtccgg gataacatta ctcgccacag ggaaaccgta cagactaatt 26640 ccagtgcgcg gaaacgataa ggactgcgtc gcggatacac gacgaccaca catagcaggc 26700 tcttgtaaac cgacgtacat cgcgccatta cgtaaggcgg cttcagcttc cgtatcgtca 26760 gccggcaacg cgtagccgta tttagccgcc agtgcgcggg cgtcctccag gctaagatag 26820 ctgtcagctc ccgcaacgat agaaccatct tcgacgacta aagccattgg ttactcctcg 26880 aacgtgcggc gacgacggcg tttaggttct gcaccaccac cgttattgtg cgcttcttcg 26940 ttatccggtt gcgttgtcac cagttcttcc ggcagaacat ttccgcgctg tggtacgagc 27000 tggccgtcaa tctctaccag accgttatat ttctcgcgga ttacgtaatt gtcattagcc 27060 attatgtttc cctatgacgg ccccgaaggg ccgtttaatt aagatactgt gacaatagtg 27120 ctgtctgaga tgatattacc ataaccatcc tgggcaacaa ctttgtaagt accggaatca 27180 gtcccggcta cggtaggcgt cttgacgtaa gtagctgctg ttgccccaga aatggcgtta 27240 ccatttttga accactgata gctgtatggc tcttggcccc ctgccgcagc tacggtgagt 27300 gtcagcgccg ccggtacggt tactgccgta gtgtcattca ggcctgtaga aaacgacgct 27360 ggggaaatgt ttaccatgtc gatttctacc tgaccatcag caggggagtc gtcggagacg 27420 cctaccatac ggcgtttaat tacatcaacc atttttaagc tcctttagct tacagttcca 27480 gcgtttttaa gcgcggttaa caggtttgct acggtagtgc gcagcgctgt gacatcggtt 27540 cggagtttgt tgtactccgc cacgagtgca tcaaattcgg ccttagtagg gtcggcgact 27600 gattgggttc ctgcggcagc agtaatagcg gctggcgagg ctacagtagc tgactttttt 27660 acgccaccaa catccgatgt agtcgccata gggatgttca gtttggcgtc tttcagactg 27720 cgtggcagcc ctttacctgt ggtgcccata catttctccc gtacgaatgt gaaaagaggg 27780 gccgtagccc ctcctgatta tacgctactg ttacgcgcct acgccagtaa ccaggaacgc 27840 gattggaacg tgtttgcgct ctactacgcg attccagttg gcggcgttcg ccaggtcctg 27900 ccaggaagcg gagcgggcga tggtttcgga gccgttaccg gtgattaccg cgctagtgaa 27960 gctgtagccc agcgggtgca gcagccaggt tttacgggtc cacagggttt caacaccgcc 28020 gccgttaccg cgtgaggctt cacgctcata ttccagtggg ttctccggag taccttcgcc 28080 gtagccgatt gcgccattgc cgaaaatgat gctgataaac ttgcggtcac tactggtgcc 28140 tacaacggtc atgctatcat caacaaccac gcggtagccc tgatatgtag caaacatggt 28200 gttattttcg gagtcacgga tgaagtcaat caggttggct ttacgtgcct ggccgtacac 28260 gaagctgtgc atcgcaatag caccgagcac ttcaccagtt gagcccatca gcgcgtcgcc 28320 catagtctgg gtggcgtcga taaaggcgcc tgcatcgaag cccagggtgg cggacacatc 28380 aataaccata tcgttctggg tgtggtactc gtcggtcgca gcaacgttat cgttgtacag 28440 gccgagagcg gtagcaatca gacgacgctg tgcctggcgc tgccagaagt tatccaggcg 28500 gttagccact gattgcagtg gattctggct ggtcagttcg acggtcaggt cagcctgccc 28560 aaaaccttcg ttcaggtacg ctacgcgcgc catcatctcg ccggtctgca cgttacgcgg 28620 tgttgcgata tcctgataca cgtcgttcga gtagttcggt tcgatagagg tatcaatcgc 28680 tttccagaaa ggaatattgg cgatgttgga cgggccgcga gcaatttcgg aagcatacgg 28740 agtaggggtc agaataccag attcaaagaa cgcggttttt tctaccgggt cctgggtcat 28800 gtaggacagc aggacgggtt cgttaccggt tacgatgtta ccaatggtgg taatagccat 28860 tattatttcc tcagggcttc aagttgccgt ttaaactcgg ctggattagt gcggtgcaac 28920 tcaaggcgct ccgcttcggt catttcacta aacattttcg cggccccgcc gcctttgcca 28980 ccggaagccc cgccaccgga agccgcatta gccttaataa gatggctgaa tgctttgtgt 29040 tcgcgcagat acttgcggaa ctgctccggg tcagtcgtaa ttacgttacc gtctggaccg 29100 acgaatttag taactacgtc atcaccttca aattctgtct tcacaaacgg tgccaggatg 29160 tctacagctt ccggcgtaat aaaatcccca gcgaagctgc caagaaccgc tttacgctcg 29220 ctgcccagta cacggttagc cagcgcggaa tacttctctt ctttctcttt cagtacaggt 29280 tcatactggc tacgaatcgt cttttcgaac tcgtccatct tacccgccga cttcagcgct 29340 tcctggtgcg ccagttgacg tgcttcttcg gcctccttcg ccttgcgcgc cgcttctttc 29400 ttctcggtca gcaacgcttc attattcgcc ttcagacccg ccacttcttt ctcaacagct 29460 tcggcaatca tctgctgcac ttgttctgcc gtgaatgtag ttgcgccgca tgccgatttc 29520 tcttcttcgc ccgcttcgtt catgagcgga aaacgtaaaa aacgattcat agtcagtatg 29580 tcccctggac gttagtagcc gggcctcccg gctttacact gtcaagaata aattattcca 29640 caggagaagg caactattcc tattgacact gttatattac tgttctaata tcagtggtaa 29700 cttaaacagg agatacttaa atgctaaagc ccgttaaaga caccgacgcg ttcaggatga 29760 caagaaaaga gctatacaat atggaacagg aatttttatc gcaatttacc gctttggaaa 29820 acgaatcctt cgagaagata atgcagctac gtgaagaagg cattttatac acaaagaccg 29880 gacctaacgc atggcaaatg cacaaaatga gaaaacctaa caagtccggc caaggggata 29940 aggagatggc aaggctgcta aaattaggta aactctatgt taaagccccg taaggggctt 30000 ttctcattct gtcacgttct taatccagtc ttgtaacctg taaaccttca gcctgagctg 30060 gcgcacgcat tccgcgttct gcacatccac agacaaatcc tcatcagcat ccgctttagc 30120 attgcctaac ttacacggcg cggtcattag ttgcggatca acctgcggca acgtgttcgg 30180 cgtgtattcc ggcgcgcaac cagttattcc aactattacg gccaacagga atagtttatt 30240 catggtcgta gccttcgatg tgattggcgt tatcgacggc ggcctgtttc agcttaacgc 30300 gcgcttcgtc gaacgtgcag acatttcggc ctggcgtttt tacgtactta acgacttcct 30360 gggtgatggt taccgtgcgg gtagcgcctt tctgttcggc ggcagctcca cgagtgtcat 30420 cctgttgctg ccgtgtgact tgcttggtta gtctggcgcg tgcctgctgg cgctgttgtg 30480 ttaccagggc ggcgcggccc tcgtcccagc ctttcccgta gtaccactcg cttacagcaa 30540 acaacgaaac aatccatagt gccgcggtaa tcataatgac tttcagattc atgttgtctc 30600 ccaacccctc gtaacagtgg gggtagctaa aagcgagccc cacaccatta ctttacggca 30660 acacactaag aatcattgtt gccgtaagtc attgaaagta tagagtccga tagattgtcc 30720 ggtccagtat aactgattca cgaaacgctg aatagactat ccccgatgtc tgtttgaccg 30780 gtgctgcgcc atacgcgcat cgcttcaggg actcaaggcc ttacggcccg tccctggcga 30840 cttgctaaga tgctatcgca tcttggcatg tcttacggtg gttaggtcaa gggcattata 30900 caaaaatgtg tcaatagttt gctaactcat ttttccgata atgcttacca gttttttaga 30960 ttataaggtt aaaaggcttt atttggggta atacttgact aataaatagt gcgtgattag 31020 tgtgtaacca gtgttgtcat tagaatcaga taaatcctaa agagggctta gcatgccgaa 31080 agttaagaat ataatggacg cgcagttacg agactgctta cgcaaaggaa tgactatttc 31140 acctgaaatg ttgaagtgag agtactgtgt tgccatttat aattaaggcc cttcacgggc 31200 cttttcttta ctctttttcc acaacttctt tcggtgtaac ctcggcagct ttcgacacgg 31260 ccttattgcc gccggcatcg gtaacgacag cccagtattt cccggcctca gtaactttta 31320 gtttctcgcc gccatcaggt acgttcacaa cctgcttgtc gtctttgtac cactgaatgg 31380 tataaggcgc cttgcctgct ttaggtgttg ccgtcagtgt ggtagccgtt gcgccgagct 31440 gcgcgtcttc agggtgtacc gcaaaataca cgtcgcctgc gtcttccaga tattctacct 31500 caaacaccat tcctgctgct gggatgggga tgcccgtttt cgctgcaaaa ggcatctcgt 31560 cgacaggtga acctaataca gattcgtcct cgatataaac cacattttcg gcagaacctg 31620 aaacgcggga atactgaaca acgcgtcgtg ccgggatatc ccgaacttta aagaaaccca 31680 tgttataact cctttaggta gtcggctact cgcttatcga gttcggccat ctgttgtagt 31740 gtaagcggtc gaccgaagcc gtcaacggaa attacgcgga actcttccgg gctgatgccg 31800 gagttacgaa atattttacc acgtaccgga cctaaagcct cgtcctggaa ccaagcaggc 31860 tgttgcttca ggaaatcgta ataggtagtg ctggcgtcaa tctgtgtacc accttctgcg 31920 ccgcgagcag cacgtttggc gcccgcatca aggaaatcat attcggagtt gataaccggc 31980 gcggtcgtac tacggcaatt atgtgctata attccgtttg caagatacca gttctgttca 32040 ttctctaggt tataaacatg cccgcaaaaa tcagcttcac cgaagaccaa gtcaacaaca 32100 tcatccagct ctacaccagt ggtgttagcc cagtcgaaat tggcaagctc atcggcattt 32160 cctgcaaccc agttatgcgg gttttgaagt cgcgcggtgt tgacatcagg agccgaagcg 32220 aggctatgtt cactcgtatg gctaacagca ctcccgaaca caggcgcgcg cttagtgctg 32280 ccgcccatga ggctgtacgg ggaagaaagg ccaaccatgc cgaactgctg cggcgcgcta 32340 aggcacggga gggtcacatt aacgccggta gtcattggga gtttatcgtt aatgatttgc 32400 tcaccaagac tggccttgac tttattccag gaaaggtggt cgacaagtac aatctcgact 32460 tcgccgtcgg acccgtcgcc gtggaagtct tcgggggtgg ttgggcttgt agtgaccgcc 32520 gaagaatcaa ccgatacctt ggccgcacta aacaaatcgg caaattcggc tacaacgtta 32580 ttttcctgat gatggagcgc aaccgcaaca tcattaccgc agaccagctt gctgccgaga 32640 ttaagcttgc cagcctcaac ccagccggta ctggtcatta cctggtgatt tggggtgata 32700 gacaaggagc gaccggactt tgtagtgata tagactatga cgcctttgta agaccgttta 32760 aaaacgttag aaacacggct accggtcgat acgacagtat ccccaagtaa acagttcgga 32820 tgtgctggcg gaagcggccc tttgccgatt tcgtatttct gaccgtcacg gctacggcag 32880 atagtcgagg ttcggctgtc cagggtggat acccattcgt atttctctac gatgtcgctg 32940 ttcttcttgt acgtcgcttc tcgcgcctgt gtcgatacgt ggttcagtgc ggtgcgaata 33000 actgttgccg cattacgctg agaaatgtcg gccaggcctc cagctccgac gacctccttc 33060 acaatctggc gtgttgtttt tccctgcacg aagcccatct taacgccggt aactaggcgg 33120 ttaacctccg tgtctcccca cccagccatc ataccaaaca ggtcggcggg cttatcatta 33180 agcgccagtg gattccactt aaccgcagac catacctggt caggcgccgg tgtgacaaac 33240 tcagcatcga cgttagctgt aagcgtcttc gtcgtccagt aagcctcgta gcccgccagc 33300 gtctgcaagt cttcggtcag cttgtcctgc cagtccgtgg ttatgccgtc caggatgtct 33360 tccaggtcgc gcagtagctt ttccaggttc ttccgcgtgc ggttgtcgtc accgaacagg 33420 agaacctgcc ggcgcacctc gtcgcgcatc tgctggatga acggcgctag ctcgttcacc 33480 tcatgcgacg cgttacgctg gagccagacc tggtggctga tgaacgactc aataagtgac 33540 attagacctc acatgtaaag agtgaccgca atcgcggcca cgatgaatat gttaaactcg 33600 gttctggtca ttcacattac cgtcattctg ttggtcaggc tggtttgcgt tggccgggat 33660 gccaccggtt accaccgtag cactcggcag cggctggtcc gctattgcgt ccatgatatc 33720 gccatccgtc cagtcagtca cgcctgcttt tcgcaatgct gcataatagg cggttgccgg 33780 tagtaatccc gcgttgatgt ctgcaaccca tgctgctcgg tcctgtgctg taattggttg 33840 gaggaagaag tccatgttca gtttgaactc aatctcgacg tcttcgcgca gccccatcat 33900 tgcggcaacc catttcagtg cgtcggtgta ggccatgctg acgttgcgcg caatggtcgc 33960 catcactgac gtatcagctc cccgctgtat ccttgcgctt tcggcggtaa tctgtctcgt 34020 cggtgagata agctgcgcgc caatctggat agcctgagtc tctttgtcca gcatattctg 34080 cttggctaga ttgtttgctt cagcctggat gagctgtgcg ctaccaccct cgccaagatt 34140 atgaccgcag cggctgccga atttaatacc gtttgggtta gcttcctgga actgctgaag 34200 actgaagtta gcgcccgtag cgatgaacag tgtgggttgg cctacaacaa aactagattc 34260 ttcgttgtcc gcgctgttgc ggaagtgtcc aatgttaagc tcggcaagcg gcagcagcgg 34320 tgcatcgtct accgtagcat cgttgttgct ggcaccgata aacgtgaacg ggattttacc 34380 ttctaattgt gggccgaggt tagggtaaat ggtctctact ggctcggcgt cgccaccgga 34440 ggccttgaac ttataaagac gctggcggta cttcccgttc tcatccaggt caagaacgcg 34500 atactgttcg ccatatttaa cgacgaattc gtcgtccgat tcgaagtatt cccacacttc 34560 acgcaagaca atcatcacga cacggttaac cgaaccgaag cgcttcatgc gccagttgat 34620 aatgttctcc gcggtgtaga atgcgatgac cgggtttaac aacccttcgt tctgttcggc 34680 agccgttgcc gcagcagtct ccggcgcatc caccagcagg ccgccgcggc caaccgagtc 34740 aatctccatc agcgtgtcct gtgcgtgttg ccataggcca acaccggaac cgtcgcagtt 34800 acgcagcagg tattccagct ccttcggaat aatctgttcc gggtctttac gcataacgct 34860 gcccaccatg ccggaaaggg tgcgcttggt gaagttgtag cagatagcgc cctgctcgta 34920 ttcggcctgg cgcgcctcgc cataggcttt atcgggctcg ttcagtccga cgttgcgcag 34980 gtaacctata aggtcacccg caacagcgtt acgcaccttc ttccatttct cgaagtggtg 35040 aagccactcg cggtgttttg ttcttacgct gttgtcgttt gccatcagct taatctctta 35100 gttaaagggc gaaagatact gggatgttaa ttacaggctt aacaatcggc atttcgtagg 35160 cgattgggta gccggtcgca tcgttctggt ggtcgttgcc gctggactta tttggctcgc 35220 cgttcttgtc gtaagcctgc tgctcaagac agcgcgacgt aaccgggcat gcctgctcgt 35280 taatcattag cataccagat tccaatgctt tattgacagc agcgacacgg tctttcaccg 35340 ccgggttagt tgacctggcg cgaatctcga atccggcctg ttgcaactgt gcgatatccg 35400 acgtcgacgc gttgttactc ttacggttct tcccgctggc gtccgggtac ataacaatat 35460 gatgcccgtt gcgcttccag cgttcactaa cggcacgtat aacgtcaggt gtgtcgtaca 35520 tctctttcag ttcggcaacc gcgtgccaga cgtgcccgcg ctgcacgtat accgtggatg 35580 ccatctgccc gacgttgaag tcctgcccta tgtaaagcgt ttcgcccggg ttaatagtct 35640 cacggctgct gtggcgctgc cggttgtagg cgtagtacac ggttccggat gtcaggttgg 35700 taaactcgcc gttgatgtac gcttcgataa gctgtgcagg atatgtcgcg cgtagagaat 35760 caacgtaatc ctccggtagg aacggattag acagcgttga cgcctgaatc atctcgtagc 35820 caggtttctt gttcactacc cagcggtcat gtacgaagcg gaatccttct ggcgtcgtga 35880 agatagacac tgtgttaact ggcttcggtg atgtagctac gtacgtatcc ggcttctgac 35940 ggttacgggc gattattttg ttccacgctt cttgtgcatg ttccattttc agcgtatcca 36000 attcgtcgac tttggcccga aatgattcat agccgacgat gcgcgccggg ttgtccagtg 36060 tacgcagtac aaaatcgcca agctgccggt tggacgtgta gatgatgtta tccgatttgt 36120 tgtagcggta gcgcacaccc cactgctgaa gcttctcttc catacgcggg gcgagaataa 36180 gacgaaccaa gtcgtaggta ggttcgtaca tagcaatcag cgacgatgaa ccaccttcca 36240 gactgtcaag gagggcggag ttacacatta cctccgattt acccgtacca aaacccgcaa 36300 cgaaggccgg gaatttacag tgcataccga ggaacgcggc ctgcggctca gtcgccttta 36360 tgcttatctg cactgacgac ctccacgact actttctgga ttggctcgtt gatatgttcg 36420 gcttcttcgc gcgcggcctt ccagccgtgg cggtcctgca tgaacaggtt cagcaggctt 36480 gtgccgatgc cggcgccggc cagctcgata gcgaaggatt gctgaagttc tcgcgccatc 36540 tggtatgctt cagcaaactc agggtagcgg cgctgccagt catccagaac tcgggtagat 36600 acaccaatct gatgacacca tctggcaaca gtcggtattt ttccggaggg aatagccttc 36660 ttgctaccct ttaaatcttc gttgatttcc cacgattccg ggtcggcaaa atagtcgacg 36720 atagcctggc agtattccgg gcggtagtta gtcgggcggc cgaacttgta gccaacgggc 36780 ttttccttat cgcctgtttt acgtgctggc gtcacaccgg caacgccttt cggcttagcc 36840 ttgccaatct tcatggggta aatctcctgt caaatgccgt tatgaccatc ataaagcatg 36900 agacgcgccc tgcgcacctc ttaggggatt atatagctag atattgacaa aggcaaaaag 36960 aagcccgcac gtggcgggca ggaggggtta aaacaaattt gataatattc cggctattcc 37020 gctgacgccc atagcggtga ccaatacgac gaccaaccac tcgggtaaat acgatagtat 37080 gttgtacctc caaatggcaa ccgctaaaaa cacccagaat actaaattgc caagtgactc 37140 taatagcccc atcataaccc gttagccctc tccgtcttgg ttatgccgcg gcgcgtgtac 37200 agcgccggac tggttttctt gtgcgtcata agacgaccgt tataaattac atgcttctcg 37260 cagtttgtgt ctaaccggta ctttgcaata gtcagctcac taaggccggt ctcacggcag 37320 gttgcggcga tagaaccaaa catcgctata agtgctggga tgctcgtcat tgtttttgta 37380 ccccacattt tacgcactgt ccccgcatgt ctttcgggtt gtagtaaaaa tcatgttcac 37440 acttattcag attgtactga ttaacaatcg cacggcgcat ctcttgaagc gatgccagga 37500 tcttctctac ctgggctatg tgctcgtcaa tctgcttcag cttgtgcccg ccttcgaaac 37560 ggtcgtaagc catggctgct gttaaacacg cctcttcgta ttcttggtta gtcatttttc 37620 ttctccagaa gtctgttaag gtatttatta tcgttcactg agccgaagct attgcgcgcc 37680 agcagttctt cgcggctcgg catcggtggc tggtttggct tggtgaactt cggggctgcc 37740 gtggtagttc catagtgctg gtaatcgtat tggtcaaaca tggtctgtct ccgggttaac 37800 gtgaatggta atcagcgttg ggtcaatgcg gcgcaggttg ccgtgttcgt cgactttcag 37860 ccagccttgc ccataaacac cggcgtcaac gtgtgcttcg gcaatggctt cgttgttgtc 37920 tgtgcgctgt ttaagtgctt taagttgcca attgcccata ctcattgccc cttatacgcg 37980 gctttaagag cctgcatcgc ggcccaccag aaagactaag gcgctaccag tccggtgctg 38040 taaacggcca agcgagccat gatttgtgcg tcttcgaacg ctagtttgtc ggtgattttc 38100 attggttact ctccagaatc tctaaagctt tagctaagta tgcctttcgt aaatcatcgg 38160 tcatagcgct attagctcgg cggatatatc cggcggcaca tttcatttga cgtttgcgca 38220 tctctaccga atcgttttct tctgcccagg ctttacactg cgcttctaat tctggggtga 38280 ttgctatggt gttcatttct cttcgacctc atgttgtatg gtgtaatcag actataggcc 38340 ggtcatagat attatgcaac tattatttgc gaataagata aaaaaaaagc cccggtagtc 38400 acgtaccggg gcaaagctgg ctgggtggaa tatttaaagt atatgactat ttaaccgcac 38460 gtccaacttt ttccgcttgt tgccacagca tcccgtcgaa cagcgccagg cgaccggttg 38520 cgcggcggcg caagccaagc agtggtttgc cgttctggtt gatgaactgc ggcagcttct 38580 ggcgcagtgt ggcgacgtcg ccagctcgta atgctttacc tgtgcccgta gacgcggcga 38640 tagcgccagg gccgacgtta aataccaggt caaccattgc gtcgaattgt gactggttta 38700 gtgacggatg cgccacagca tcaacagctt taacggcagc agccatatcc ttgttcagca 38760 gaataagacc ctgaccttcg gtaatcttct ggccctcttt gacgtgcggg ccgtagctgc 38820 cgtacccaat tgtccagtat ttctcgttct tagtcgcctt gtaagctgtg ccgcggaatt 38880 gctcgaatgc cgcggtgaaa tgcaggccat tatctgagat gtttcgcata tttcctccgg 38940 tatttaaaat atctcatcga tttcaccgcc aggcttcgaa taccaagcag gcagaaaatg 39000 acgaagattg tcggcgtgag gtgttcaggg tcccctgcgc tggcatgtcg aaataacccc 39060 acggtacaag tcagcgccag ggccatataa agtaagcgcc cgaccagacc atcttcaatc 39120 cagtgggcgt aaatattgat gagggcaact ccccccataa ccaccagaca gataatacct 39180 agtggggtgt tcaggaaagt agctggcatt ttatttactc cacggtaacc tgatgttgcc 39240 gatggatgca tctgcggctt cgaatagctt caaccagaac ataccaacgc caaaaggcac 39300 caggtactga ccatcagaat ttgatagttc atagtagctg ataatcacag gggatgcata 39360 cacggcacag gcggcgccag cagccagatg gcaaagtcgg cgccatagtg ggatagtctc 39420 gcctttctta atttgggcta cggcaccgcc gcaaatggct gctactgcta gccagaagtg 39480 tttatcgtcc atcagtgaga ggactccgta atggttagtt tcattacgga ggttaacaga 39540 ttgtcaatag agaatcactt atttttagga tttatctgag tagttacgca ttgtgtatag 39600 tctcctttct ggcttgttag gtatgttgtc gaagccgcat ttacgccagt acatataaat 39660 cctgcgagca attaaagctc tagcctgcgt atgaaacatg ccaatgcgta ttgtttttat 39720 tccgtcgtaa aaataagcgt cccatgtgtt attgcgcgag ttgaaagcaa ctccggcaac 39780 gtgctcatgt tttggagcta cgtaattaac agtcttaccc ttcttcctgt ttaaccttgg 39840 acctttaggt gcagctcgtt tcgccttttt gcgtatggca ggacccatat agtcagcccg 39900 tattgattta gatgggggcg aaaggtcgga gttagcacgt ttcagtatgt cagttgccag 39960 actcatggca ctaccctccc acaacaaggg cacttaccct taacagttaa tgcgtatgta 40020 ttggcgcatc ttcttgtctc aggtttacgc gatgagtcca gttcccgcgc gcgcaacgcg 40080 ctatcaacga tgtcctgcaa gtcctcggca gcgtctttat gcccgcgctg cccgaccgcc 40140 agggctttct taatcaggtg ctgcaacgca gggtttctca catcccaggc cttcaggacg 40200 tcgtatacgt cgacgaatac gcctggctta atctcacggt gatatttatt gttggacata 40260 agggaattct ccggttctgt accattcatc tagtaattca tcctgttcct tgcggtcggc 40320 ggcgcgttct tcgtcagaca cctagcaccc cctttgccag ttcttctgtg tccgggctta 40380 acggatagtt cagtactgaa tacgccaggg cgtcaagtag ctctttcagt tccgcataat 40440 cctcgtatct aacccattca tctcgttcag tttcgcaata ttgctctcct gcgtcagttt 40500 caaacatctc ctgcacatag ttaaacctat accgtgcaac cattctcact tatccttact 40560 cagtcgtttc gtgttacaga aagcccttac ctgtacgtct gaacgctcgg caaaggccat 40620 aggtgcattc tcggcgttac gtgctgccgt ctggcaagcc tccagggtgt agaacgtctc 40680 cacgccctcc agatgcacct ggcctgcgtt aagtacccag atgaaaagaa cactggtcat 40740 atattcacct tagataaatc gaagtctata ggtaaactct ttttaaaccc acattccggg 40800 cattcaaaag ttcggtcctt agcattgata tatgtatttt tatgctcgca caactgcgta 40860 tatggcttac ggcattctgc ttgttcaatc ccctctagct ttcgaatcca tcccgcgtga 40920 gtttctgcaa tcgctttgta atcatcgcgc tcctgtttca acttcgcatt actcgcacgc 40980 aactgcttaa cagtattttc aagctccttg acgcggcgct cggcagcacg tttagaatca 41040 cgggcaccat ccagatgcga ctgcatgttc tgcaaggtca tcttaacttc atccatgtcg 41100 gccagattaa tgttgacatt gaagcgtgtt gctttaggcc ggcacgcctc caagtctccg 41160 gccagggcct tgtcgttaag tccaggggtg gttgcgtcgt ttacttctcc aataacatag 41220 gtcttgttat ccaggataaa tccgccaccg accatataac caagcacatc atgcatcgca 41280 tgagccagtc gtgacgcgga ggcaggaata ccgctgccgg gtgcgagcag gtcaaccata 41340 gcctgctggt ggtcttttag ggtagccatt attttacctc accttttggc acgcaaaatt 41400 tctgcgtgaa actagcactg tcatctttct ttttgacttc ttgcactgcg gcttcacacg 41460 cttgcaggtt attaaattcg gcggtagcaa cagcattacc acccataccg aacat 41515

Claims

삭제
삭제
삭제
박테리오파지 TS3, 박테리오파지 TS6 및 박테리오파지 TS13의 혼합물 (KACC 97036P)을 포함하는 살모넬라 사멸용 조성물.
박테리오파지 TS3, 박테리오파지 TS6 및 박테리오파지 TS13의 혼합물 (KACC 97036P)을 포함하는 식품 첨가제.
박테리오파지 TS3, 박테리오파지 TS6 및 박테리오파지 TS13의 혼합물 (KACC 97036P)을 포함하는 식품.
박테리오파지 TS3, 박테리오파지 TS6 및 박테리오파지 TS13의 혼합물 (KACC 97036P)을 포함하는 사료 첨가제.
박테리오파지 TS3, 박테리오파지 TS6 및 박테리오파지 TS13의 혼합물 (KACC 97036P)을 포함하는 사료.