KR101101377B1 - 이질균에 특이적 사멸능을 갖는 박테리오파지 - Google Patents

이질균에 특이적 사멸능을 갖는 박테리오파지 Download PDF

Info

Publication number
KR101101377B1
KR101101377B1 KR1020090098650A KR20090098650A KR101101377B1 KR 101101377 B1 KR101101377 B1 KR 101101377B1 KR 1020090098650 A KR1020090098650 A KR 1020090098650A KR 20090098650 A KR20090098650 A KR 20090098650A KR 101101377 B1 KR101101377 B1 KR 101101377B1
Authority
KR
South Korea
Prior art keywords
bacteriophage
present
ala
dysentery
gly
Prior art date
Application number
KR1020090098650A
Other languages
English (en)
Other versions
KR20110041698A (ko
Inventor
배진우
김경호
장호원
Original Assignee
경희대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 경희대학교 산학협력단 filed Critical 경희대학교 산학협력단
Priority to KR1020090098650A priority Critical patent/KR101101377B1/ko
Publication of KR20110041698A publication Critical patent/KR20110041698A/ko
Application granted granted Critical
Publication of KR101101377B1 publication Critical patent/KR101101377B1/ko

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065Microorganisms
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/76Viruses; Subviral particles; Bacteriophages
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00Bacteriophages
    • C12N2795/00011Details
    • C12N2795/00021Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00Bacteriophages
    • C12N2795/00011Details
    • C12N2795/00031Uses of virus other than therapeutic or vaccine, e.g. disinfectant
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00Bacteriophages
    • C12N2795/00011Details
    • C12N2795/00032Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Genetics & Genomics (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)

Abstract

본 발명은 이질균에 특이적 사멸능을 갖는 박테리오파지에 관한 것으로 구체적으로 본 발명은 기탁번호 KCTC 11432BP를 가지는 이질균에 특이적 사멸능을 갖는 박테리오파지, 상기 박테리오파지 또는 이의 배양물을 유효성분으로 함유하는 항균제, 이질균에 의해 유발되는 질환의 예방 또는 치료용 약학적 조성물, 사료첨가제 또는 식품첨가제에 관한 것이다. 본 발명의 박테리오파지는 이질균을 특이적으로 사멸시킬 수 있어, 상기 박테리오파지 또는 이의 배양물은 항균제, 이질균에 의해 유발되는 질환의 예방 또는 치료용 약학적 조성물, 사료첨가제 또는 식품첨가제로 효과적으로 사용될 수 있다.
이질균, 시겔라, 박테리오파지, 항균제

Description

이질균에 특이적 사멸능을 갖는 박테리오파지{Bacteriophage having killing activity specific to Shigella}
본 발명은 이질균에 특이적 사멸능을 갖는 박테리오파지에 관한 것으로 구체적으로 본 발명은 기탁번호 KCTC 11432BP를 가지는 이질균에 특이적 사멸능을 갖는 박테리오파지, 상기 박테리오파지 또는 이의 배양물을 유효성분으로 함유하는 항균제, 이질균에 의해 유발되는 질환의 예방 또는 치료용 약학적 조성물, 사료첨가제 또는 식품첨가제에 관한 것이다.
이질균은 시겔라(Shegella)속에 속하는 균으로 장내세균과에 속하는 세균이다. 이질균은 운동성이 없고 협막도 없으며 아포도 형성하지 않는 그람음성 세균으로 S.flexneri, S.sonnei, S.doydii, S.dysenteriae의 4종으로 나뉜다. 상기 균들은 세균성 이질을 발생시키는 데, 상기 질환은 감염력이 강해 매개체 내의 증식없이도 환자나 보균자의 손에 묻어있는 세균만으로도 전파가 가능하며, 점촉감염증이 시겔라 감염증의 주된 전파 방법이다. 세계적으로 매년 2억명의 발병을 보이고 있으며 그중 65만명이 사망하고 있는 실정이다. 우리나라에서 세균성 이질의 발병은 감소추세를 보이다 1998년이후 전국적으로 집단 발병하는등 발병이 현격히 증가하 여 사회문제화 되고 있다. 또한 1998년 이후 분리된 이질균의 경우 테트라싸이클린, 스트렙토마이신등 항생제에 대하여 내성을 보이는 등 항균제에 대한 내성이 심각한 수준으로 대두대고 있다.
상기 이질균에 감염된 경우 치료를 위하여 주로 항생제를 사용한다. 하지만 항생제가 남용되는 경우 항생제 내성 미생물들이 발생하여 부작용이 발생할 수 있다. 구체적으로 항생제가 남용되는 경우 미생물들은 항생제에 대한 내성을 획득하게 되었고 내성을 지닌 미생물들은 다른 미생물에게 내성을 전달하여 확산 속도가 빠르게 퍼져나갔다. 따라서 상기 항생제가 아닌 다른 방법으로 이질균을 사멸시킬 수 있는 방법이 필요한 실정이다.
한편, 박테리오파지(bacteriophage)는 세균에 특이적으로 침투하여 용균하는 바이러스로 유전물질 (single DNA, double DNA, RNA)을 단백질 외피가 싸고 있는 유기체다. 상기 박테리오파지는 자연에 존재하는 모든 생물체를 감염시킬 수 있으며, 생물이 존재하는 거의 모든 곳에 함께 존재할 수 있다. 뿐만 아니라 그 수와 다양성도 엄청난 것으로 밝혀지고 있고, 실제로 해양이나 토양의 경우 박테리아의 수보다 많이 존재하는 것으로 추정되고 있으며 그 다양성도 어마어마한 것으로 알려지고 있다. 이들을 이용한 파지 치료법 (phage therapy)은 인간뿐만 아니라 식물, 동물, 음식 등 그 응용분야가 매우 다양할 뿐 아니라 잠재적 응용성도 많다고 할 수 있으며 나아가 항생제보다 보다 특이적인 치료가 가능하다.
이에 본 발명자들은 이질균에 특이적 사멸능을 갖는 박테리오파지에 대하여 연구하던 중, 하수로부터 신규한 박테리오파지를 분리하고 상기 박테리오파지가 이질균에 특이적 사멸능이 있음을 밝혀 본 발명을 완성하였다.
따라서 본 발명은 이질균에 특이적 사멸능을 갖는 박테리오파지 (기탁번호 KCTC 11432BP)를 제공하는 것을 목적으로 한다.
또한 본 발명은 상기 박테리오파지 또는 이의 배양물을 유효성분으로 함유하는 항균제를 제공하는 것을 목적으로 한다.
또한 본 발명은 상기 박테리오파지 또는 이의 배양물을 유효성분으로 함유하는 이질균에 의해 유발되는 질환의 예방 또는 치료용 약학적 조성물을 제공하는 것을 목적으로 한다.
또한 본 발명은 상기 박테리오파지 또는 이의 배양물을 포함하는 사료첨가제를 제공하는 것을 목적으로 한다.
또한 본 발명은 상기 박테리오파지 또는 이의 배양물을 포함하는 식품첨가제를 제공하는 것을 목적으로 한다.
상기 과제를 해결하기 위하여, 본 발명은 이질균에 특이적 사멸능을 갖는 박테리오파지 (기탁번호 KCTC 11432BP)를 제공한다.
또한 본 발명은 상기 박테리오파지 또는 이의 배양물을 유효성분으로 함유하 는 항균제를 제공한다.
또한 본 발명은 상기 박테리오파지 또는 이의 배양물을 유효성분으로 함유하는 이질균에 의해 유발되는 질환의 예방 또는 치료용 약학적 조성물을 제공한다.
또한 본 발명은 상기 박테리오파지 또는 이의 배양물을 포함하는 사료첨가제를 제공한다.
또한 본 발명은 상기 박테리오파지 또는 이의 배양물을 포함하는 식품첨가제를 제공한다.
이하 본 발명을 보다 상세히 설명한다.
본 발명에서 항균제는 미생물의 성장을 억제할 뿐 아니라 미생물을 파괴 또는 무력화하는 제제를 의미한다.
본 발명에서 배양물은 본 발명의 박테리오파지의 배양액, 상기 배양액의 농축액, 상기 배양액 또는 농축액의 건조물을 포함한다. 상기 배양물은 본 발명의 박테리오파지를 당업계에 공지된 박테리오파지 배양용 배지에 본 발명의 박테리오파지를 접종하여 제조될 수 있으며, 상기 배지는 이에 한정되지 않지만 TSA(Tryptic Soy Agar)일 수 있다.
본 발명의 박테리오파지는 이질균이 서식하는 곳에서 당업계에 공지된 방법에 따라 분리될 수 있다. 이에 한정되지 않지만 바람직하게는 박테리아는 여과하지 만 박테리오파지는 통과시킬 수 있는 구멍크기의 필터로, 이질균이 서식하는 곳에서 채취한 시료를 여과시킨 다음, 원심분리하여 상등액을 회수하고 상기 상등액을 이용하여 용균반 분석(plaque assay)을 함으로써 이질균을 특이적으로 사멸시킬 수 있는 본 발명의 박테리오파지를 분리할 수 있다 (<실시예 1-1> 참조).
본 발명의 박테리오파지는 형태학적 분류상 미오비리대 (Myoviridae) 및 T4 유사 파지에 속하며, 본 발명에서 상기 박테리오파지를 ‘bacteriophage SP18’로 명명하고 2008년 11월 21일자로 생명공학연구소 유전자은행(korean collection for type cultures, KCTC)에 기탁번호 KCTC 11432BP로 기탁하였다 (<실시예 1-2> 참조).
본 발명의 박테리오파지는 30분 정도의 잠복기를 가지는 생장 특성이 있다 (<실시예 1-3> 참조).
본 발명의 박테리오파지는 서열번호 1의 염기서열로 표시되는 유전체를 가지며, 상기 염기서열은 gDNA 서열이다. 또한 본 발명의 박테리오파지의 외피 단백질 중의 하나인 g23 단백질 아미노산 서열(서열번호 2)을 다른 박테리오파지와 비교한 결과, 본 발명의 박테리오파지는 다른 박테리오파지와 동일하지 않으며 신규한 것이다 (<실시예 1-4> 참조).
본 발명의 박테리오파지는 이질균에 특이적 사멸능을 가지는 것을 특징으로 한다. 상기 시겔라속 균은 이에 한정되지 않지만 바람직하게는 시겔라소네이(Shigella sonnei) 균일 수 있다. 구체적으로 본 발명의 박테리오파지는 이질균에 대해서 특이적인 사멸효과가 있으며, 에스케리키아 (Escherichia) 및 살모넬라 속 균에 대해서는 사멸효과가 없다 (<실시예 2> 참조).
한편, 본 발명의 항균제는 박테리오파지 또는 이의 배양물을 유효성분으로 함유하는 것을 특징으로 한다.
상기 박테리오파지 또는 이의 배양물은 상기 기재한 바와 같이 이질균에 특이적 사멸능이 있어, 상기 성분을 함유하는 항균제는 이질균을 사멸시킬 수 있는 특성이 있다. 상기 시겔라속 균은 이에 한정되지 않지만 바람직하게는 시겔라소네이(Shigella sonnei) 균일 수 있다.
상기 이질균은 주로 식품 또는 화장품을 오염시킬 있다. 식품은 미생물의 탄소원 및 질소원 등 수 많은 유기물, 무기물을 함유하고 있으며, 화장품은 기름이나 물을 주성분으로 하며, 미생물의 탄소원이 되는 글리세린이나 솔비톨 등과 질소원이 되는 아미노산 유도체, 단백질 등이 배합된 것이 많아, 상기 이질균이 번식하기 용이하기 때문이다. 따라서 본 발명의 항균제는 상기 식품이나 화장품에 첨가되어 사용될 수 있다.
본 발명의 항균제는 유익한 균은 사멸시키지 않고 병원성 균인 이질균만을 선택적으로 죽일 수 있어, 부작용이 없으며 병원성 균의 내성 내지 저항성을 유도하지 않아 기존의 항생물질에 비하여 효과적으로 이용될 수 있다.
본 발명의 항균제는 통상적인 항균제 제조방법으로 제조될 수 있으며, 상기 본 발명의 항균제는 유효성분인 본 발명의 박테리오파지 또는 이의 배양물 외에 필요에 따라 담체, 기타 보조제 등을 추가적으로 포함할 수 있는데, 상기 담체는 생 리식염수, 증류수, 사료, 박테리아용 배지(예, Tryptic Soy Agar) 등일 수 있으며, 보조제는 pH 교정 등의 목적으로 완충액 등일 수 있다. 또한, 본 발명의 항균제는 액상, 반 고체상 또는 고체상으로 제제 형태는 분말제, 과립제, 정제 등의 고체상 제제, 현탁제 또는 주사액제 등의 액상 제제 및 크림, 로션, 젤, 페이스트 등의 반 고체상 제제 등으로 제형화될 수 있다.
한편, 본 발명의 약학적 조성물은 상기 박테리오파지 또는 이의 배양물을 유효성분으로 함유하는 이질균에 의해 유발되는 질환의 예방 또는 치료용인 것을 특징으로 한다.
상기 박테리오파지 또는 이의 배양물은 상기 기재한 바와 같이 이질균에 특이적 사멸능이 있어, 상기 성분을 유효성분으로 함유하는 본 발명의 약학적 조성물은 이질균에 의해 유발되는 질환의 예방 또는 치료용으로 효과적으로 사용될 수 있다. 상기 시겔라속 균은 이에 한정되지 않지만 바람직하게는 시겔라소네이(Shigella sonnei) 균일 수 있다.
상기 이질균에 의해 유발되는 질환은 이에 한정되지 않지만 바람직하게는 세균성 이질일 수 있다.
본 발명의 약학적 조성물은 상기 질환 부위에의 도포 또는 분무하는 방법으로 이용할 수 있으며, 그 밖에 경구 또는 비경구 투여를 통해 투여할 수도 있으며, 비경구 투여의 경우 정맥내 투여, 복강내 투여, 근육내 투여, 피하 투여 또는 국부 투여를 이용하여 투여할 수 있다.
본 발명의 약학적 조성물의 적합한 도포, 분무 및 투여량은 제제화 방법, 투여 방식, 환자의 연령, 체중, 성, 질병 증상의 정도, 음식, 투여 시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하며, 보통으로 숙련된 의사는 소망하는 치료에 효과적인 투여량을 용이하게 결정 및 처방할 수 있다. 일반적으로, 본 발명의 약학적 조성물은 이에 한정되지 않지만 바람직하게는 1x103 내지 1x1010 pfu/ml의 박테리오파지를 포함할 수 있다.
본 발명의 약학적 조성물은 당업자가 용이하게 실시할 수 있는 방법에 따라, 약리학적으로 허용 가능한 담체, 부형제, 희석제 또는 부성분을 추가로 포함할 수 있다. 상기 약학적으로 허용되는 담체, 부형제 또는 희석제의 예로는, 락토즈, 덱스트로즈, 수크로즈, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말티톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로즈, 폴리비닐 피롤리돈, 물, 메틸하이드록시벤조에이트, 프로필하이드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유, 프로필하이드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유, 덱스트린, 칼슘카보네이트, 프로필렌글리콜, 리퀴드 파라핀 및 생리식염수로 이루어진 군에서 선택된 1 이상을 들 수 있으나, 이에 한정되는 것은 아니며 통상의 담체, 부형제 또는 희석제 모두 사용 가능하다. 또한, 상기 약학 조성물은 통상의 충진제, 증량제, 결합제, 붕해제, 항응집제, 윤활제, 습윤제, pH 조절제, 영양제, 비타민, 전해질, 알긴산 및 그의 염, 펙트산 및 그의 염, 보호성 콜로라이드, 글리세린, 향료, 유화제 또는 방부제 등을 추가로 포함할 수 있다.
한편, 본 발명의 사료첨가제 또는 식품첨가제는 상기 박테리오파지 또는 이의 배양물을 포함하는 것을 특징으로 한다.
상기 박테리오파지 또는 이의 배양물은 상기 기재한 바와 같이 이질균에 특이적 사멸능이 있어, 상기 성분을 유효성분으로 함유하는 본 발명의 사료첨가제 또는 식품첨가제는 이질균을 특이적으로 사멸시킬 수 있다. 상기 시겔라속 균은 이에 한정되지 않지만 바람직하게는 시겔라소네이(Shigella sonnei) 균일 수 있다.
상기 식품첨가제가 사용될 수 있는 식품에는 제한이 없으며, 상기 식품첨가제는 이질균에 감염되기 쉬운 식품에 당업계에 공지된 방법에 따라 첨가제의 형태로 제조된 것을 말한다. 상기 사료첨가제는 이에 한정되지 않지만 가축용 및 어류용 사료에 첨가제의 형태로 제조된 것을 말한다. 상기 사료첨가제 또는 식품첨가제의 첨가량은 당업자라면 용이하게 정할 수 있으며, 이질균의 감염 용이성, 감염 정도에 따라 달리 정할 수 있다.
본 발명의 박테리오파지는 이질균을 특이적으로 사멸시킬 수 있어, 상기 박테리오파지 또는 이의 배양물은 항균제, 이질균에 의해 유발되는 질환의 예방 또는 치료용 약학적 조성물, 사료첨가제 또는 식품첨가제로 효과적으로 사용될 수 있다.
이하, 본 발명을 실시예에 의해 상세히 설명한다.
단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.
< 실시예 1>
본 발명의 박테리오파지의 분리 및 동정
<1-1> 본 발명의 박테리오파지의 분리
이질균을 특이적으로 사멸시킬 수 있는 박테리오파지를 분리하기 위하여, 이질균이 존재할 것으로 예상되는 곳에서 시료를 채집한 후 이의 배양액을 이질균에 접종하여 이질균을 사멸시키는 박테리오파지를 분리하였다.
구체적으로 박테리오파지가 존재할 것이라 예상되는 하천수에서 검체를 채집하여 여과 종이로 일차 여과한 후에, 회수된 여과액을 0.22 ㎛의 필터를 이용하여 여과한 다음, 여과액을 이질균과 함께 30℃에서 1일 동안 배양하고 다시 8,000 rpm에서 20분간 원심분리하여 상등액을 회수하였다. 상기 회수된 상등액을 0.22 ㎛의 필터를 이용하여 다시 여과한 다음, 그 여과액을 이용해 용균반 분석(plaque assay)( Calson, K. (2004) Appendix. Working with Bacteriophages:Common Techniques and Methodological Approaches. In the Bacteriophages:Biology and Applications (Kutter, E & Sulakvelidze, A eds) pp 437-494, CRC Press, Florida)을 함으로써 이질균을 특이적으로 사멸시킬 수 있는 박테리오파지를 분리하였다.
<1-2> 본 발명의 박테리오파지의 형태학적 분류 및 기탁
상기 <실시예 1-1>에서 수득한 박테리오파지의 형태학적 특성을 살펴보기 위해, 상기 박테리오파지를 10% 폴리에틸렌 글리콜 8000에 넣은 후 4℃에서 하룻밤 동안 클로로포름으로 이질균과 파괴된 세포 잔해를 제거하였다. 이 후 염화세슘(CsCl) 밀도 구배법(밀도: 1.15 g/㎖, 1.45 g/㎖, 1.50 g/㎖ 및 1.70 g/㎖)을 이용한 원심분리(20,000 rpm, 2시간, 4℃)를 통하여 박테리오파지를 정제하였다.
상기 정제된 박테리오파지를 구리 그리드(copper grid)에 묻고, 2% 우라닐 아세테이트(uranyl acetate)로 대조 염색(negative staining) 및 건조한 다음, 투과전자현미경을 이용하여 형태를 관찰하고 그 결과를 도 1에 기재하였다.
상기 도 1에 나타낸 바와 같이, 본 발명의 박테리오파지는 형태학적 분류상 미오비리대 (Myoviridae) 속 및 T4 유사 파지에 속하는 것을 알 수 있고 도 3에 나타낸 바와 같이 계통학적으로 T4 유사 파지에 속하는 것을 알 수 있었다.
상기와 같이 분리한 박테리오파지를 'bacteriophage SP18'로 명명하고 2008년 11월 21일자로 생명공학연구소 유전자은행(Korean Collection for Type Cultures, KCTC)에 기탁번호 KCTC 11432BP로 기탁하였다.
<1-3> 본 발명의 박테리오파지의 생장 특성
본 발명의 박테리오파지의 생장특성을 살펴보기 위하여 생장곡선을 작성하였다. 구체적으로 one-step growth curve test를 실시하였으며, 보다 구체적으로 이 질균을 TSB (tyrptic soy broth, Difco)에 접종한 후 30℃에서 exponential time (OD 0.2-0.3)까지 배양한 후 MOI (multiplicity of infection) 0.1 수준의 본 발명의 박테리오파지를 접종 한 후 30℃에서 10분 간격으로 배양액을 추출하고 OD와 박테리오파지의 수(plaque form unit, PFU)를 측정하여 그 결과를 도 2에 기재하였다.
상기 도 2에 기재한 바와 같이, 본 발명의 박테리오파지는 30분 정도의 잠복기를 가지며, 접종 후 한 시간 후부터 감염된 배양액에서 OD 수치가 감소하는 것을 알 수 있다.
<1-4> 본 발명의 박테리오파지의 유전자 서열 분석 및 비교
1L의 TSB에서 30℃에서 배양한 후 본 발명의 박테리오파지를 접종하여 용균 현상이 관찰될 때 폴리에틸린글리콜 침전 및 염화세슘 고속원심분리를 통하여 박테리오파지를 농축하였다(Calson, K. (2004) Appendix. Working with Bacteriophages: Common Techniques and Methodological Approaches. In the Bacteriophages: Biology and Applications (Kutter, E & Sulakvelidze, A eds) pp 437-494, CRC Press, Florida). 상기 농축된 박테리오 파지에서 DNA를 분리하여 (Sambrook, J. & Russell, D. W. (2001). Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, New York.) 박테리오파지의 전체 유전체 서열 (170605개의 DNA 서열)을 밝혀 서열번호 1에 기재하였다.
또한 본 발명의 박테리오파지의 외피 단백질 중의 하나인 g23 단백질의 아 미노산 서열(서열번호 2)을 다른 박테리오파지와 비교한 결과, 도 3에 기재한 바와 같이 본 발명의 박테리오파지는 엔테로파지 JS98 (91%의 DNA 상동성) 등 다른 박테리오파지와 동일하지 않으며 차이가 있음을 알 수 있다.
< 실시예 2>
본 발명의 박테리오파지의 이질균주에 대한 특이적 사멸 효과
본 발명의 박테리오파지의 숙주에 대한 감염성을 조사하기 위하여, 이질균과 분자 계통학적으로 가장 가까운 균을 숙주로 하고 실험을 수행하였다. 상기 균주를 TSA(tryptic soy agar, Difco) 배지에 배양하고 페이퍼 디스크(paper disc)를 가운데 놓은 후, <실시예 1-2>에서 정제된 박테리오파지 30ul를 디스트 위에 접종하였다. 상기 접종 후, 30℃에서 24시간 동안 배양한 후, 환이 생기면 본 발명의 박테리오파지의 용균(lysis) 활성이 있는 것으로 나타냈으며, 상기 결과를 하기 표 1에 기재하였다.
본 발명의 박테리오파지의 이질균주에 대한 특이적 사멸 효과
번호 기탁번호 사멸여부
1 KCTC2293 Escherichia coli -
2 KCTC2441 Escherichia coli -
3 KCTC2790 Escherichia coli -
4 DSM4481 Escherichia blattae -
5 DSM4560 Escherichia hermannii -
6 DSM4564 Escherichia vulneris -
7 DSM13698 Escherichia fergusonii -
8 KCTC2518 Shigella sonnei +
9 KCTC2009 Shigella sonnei +
10 DSM5570 Shigella sonnei +
11 DSM 14848 Salmonella choleraesuis indica -
12 DSM 14846 Salmonella choleraesuis choleraesuis -
13 DSM 13772 Salmonella bongori -
14 DSM 9386 Salmonella choleraesuis arizonae -
15 DSM 9221 Salmonella choleraesuis hountenae -
16 DSM 9220 Salmonella choleraesuis salamae -
17 ATCC 13311 Salmonella typhimurium -
18 ATCC 13076 Salmonella enteritidis -
19 DSM 14847 Salmonella choleraesuis diarizonae -
- KCTC: the Korean Collection for Type Cultures
- DSM: the German Collection of Microorganisms and Cell Cultures
- ATCC: the American Type Culture Collection
상기 표 1에 나타낸 바와 같이, 본 발명의 박테리오파지는 이질균을 특이적으로 사멸시킬 수 있음을 알 수 있다.
도 1은 본 발명의 박테리오파지를 투과전자현미경으로 관찰한 사진이다.
도 2는 본 발명의 박테리오파지의 생장 특성을 나타낸 one-step 생장곡선이다. (○: 박테리오파지가 감염되지 않은 배양액의 OD값, ●: 박테리오파지가 감염된 배양액의 OD값, ◆: pfu/ml를 나타냄)
도 3은 본 발명의 박테리오파지 (SP 18)의 외피 단백질 중의 하나인 g23 단백질의 아미노산 서열을 다른 박테리오파지와 비교하여 계통학적 위치를 나타낸 것이다.
SEQUENCE LISTING <110> INDUSTRY ACADEMIC COOPERATION FOUNDATION KYUNGHEE UNIVERSITY <120> Bacteriophage having killing activity specific to Shigella <130> DPP20094091KR <160> 2 <170> PatentIn version 3.2 <210> 1 <211> 170603 <212> DNA <213> bacteriophage SP18 <400> 1 attattcctt attggccgct tccacggcct tcatgatttg agcaacgttc tgcacttgtt 60 cagttgacat ggcgttacgg ttatataaaa agtgtgaaac aattgggtga ttcttttcaa 120 attcaaaaac cattttatcg cttgttaccg aagcgttttt ggtcaggtcg tcataaatct 180 tattacaaag gaccagcgtt tcctgcatgg tcttatcaac cgttccactg ttatatgcgt 240 tataagcaaa gaacttattg aactgcatta gttcggcata gtcagtattc acatctttct 300 gaacatcttt tgcataaaaa tacttgaaca taaaattcag ttctgcatat tcagacagga 360 taggaatata ataacgcgaa cggcttgaag cggcaatgta ctggtccgca tcaaccgcat 420 caatcaattc cacataacga ttgactgcag cttcaatcag acaatcatac tgcgccagct 480 tggtaacctt tttctgaagc tgtggacgaa taacacagaa ttcattgatt tccaacagat 540 tcgccagttt gcacaatgtc tgggtcccga aggtcgtggt gccatattcc tgattcaacg 600 gggaaatgct actgcggtta atgaatacag cataaccatc gatttcctct gcatcagctg 660 cacatgtaaa ttcttcaaca gactgccatt tgccattggt tttagtccaa cgaagcaatg 720 atggagtctt tgacttaacg cgggctttcg ggtcagcaac cggaatccag tcttttacaa 780 gggcagtcag ttcagacgta cgatagatat taaccgtatc gccttcaaat cgttcctgga 840 gttgaaccat caaccgtgtt tgaatttcgc tggttgggtc aacgaacaat agactactac 900 cttgtacagt cggaacttta ccggatgctt tgagcttttt ggctttcgcg ctggtgtcat 960 acagcattga actgatacca cgaatcatcg caattcgccc tttagcatcg tcaataacaa 1020 tattcaaggt atcattatga ataccaaaca tgcttgtcag gcttgcagta ttcgtgttcg 1080 agcttgagtt acgcagacgt ttcagacggg tggtgctata gatatcatag acaaccccgg 1140 cattaataag gtctttgtcc agctcgaaca tttcgtacat ctgattaaag gtcaattgtg 1200 atttcgtaaa tttcgtttca acattacgag ctttcagaaa attacgagct tcataattca 1260 gattctgcaa ttcgcgatac gtatggcgga tacattctga ttcctgccat ttcttacaat 1320 catcttccat gaccttctta tcaagcgatt caatacgttc aacgatattt gcgaccgtac 1380 gtttgtccag tgataatgct tcgcgagaag gtgcaatatc caacgaaccc atcgggaact 1440 taataaagac aacatcattt ttggcagtca tccaggtttt cttggtgtaa ccatcaccaa 1500 tcggataaac aattccgcca taaattgcat aaagtccata acgttctgga ctgctataat 1560 taatacgcgc tggagcatag taatcatcaa attccgggaa gtattcaatt tcgccagcgc 1620 cttgcacttc agccagtcca gcaaatggac gcatgatata tgcaatctcg tagcggaact 1680 taccgaaatc attactatcg accgggacaa caacttcaat ccctgtgcgg tcaccaggtt 1740 tcattggttc gctaaaggtt ggcttaatct gtggaccatc gccgtccata taagcgacat 1800 aaccattaac ttgtccatca tgatatgaaa tcaggctaaa cgtctctgta taactgaacg 1860 gagatttaga acccagacca aagccaccaa taaaatcgtt ggattcagcc ttggatgatg 1920 caaagaaact gttatacaaa cctggagtat tttcatcacc acgaatctgg aaatcactca 1980 tacccggacc aaagtcacga catacaaaac gtgggtccag ttcaccagga gcttgcacaa 2040 tgaatttatc ctgacagcca ttgagcatat gagcatcaat catattggta atcagctcac 2100 gaacaaccgc gcgcaccttg tttgtataaa ggtcgctggt cagaatctta aacacctttg 2160 gagatgcctg gattgtgaat gctgatgcct tggcaccatt accaaggatt gcttcctttt 2220 cagtttctaa aatcatgttt ttctcacttt atgttacgtt tgattaattc tgcaacttca 2280 agaagttcat cctttgttgc ggcgtctgaa gctattttaa tacggatttc tttgaagcgt 2340 tctttaaact cttcagcttc tcttatatca aaaaatcgtt ggataacacg gtactcttta 2400 tggatagctt tatcaaacag ttccatatta actttgaagt tcgtcatttc caatctcctg 2460 taatattttt ctcatactac gcatggtagc tttatgttct tggtcatggt atatcaatgc 2520 aaggagtgct tcaattcgct cctcagggac cttctggcca gccttaatgc tcatggctgt 2580 tgttcttaca gctaataagt aacttcgctt cctgtcgcta tctgggagct cctgtacctt 2640 cctcaggcct gcttccataa tacaccgtgc gacattttgc agattaacta aacttccgtc 2700 agacataatc atattttcac ctcaaagaca tcttaacaca atttttgtgg cttgtacata 2760 ataaaaaggt ctcccgaagg agaccaaatt ttattctact ttatagactt gaccatacgc 2820 catgaaagtt tcaccttccc aggtttcgca cgtgattgtt acaaattctg caaacaaagg 2880 ctttttctct gcccagagaa aaccatattc accggtagcg agtgtaatcc agtatttagt 2940 catattgtct cctagtagcg ggagaatatt ctcccacctc tatttatagc gtcgcattca 3000 tccaatcttt gcgctgctgg gaatctttac cgaatagcat ttcgaacagt tctttccagt 3060 tgtcgggcaa ttgaacaact tcgaataccg gttcaagcaa tacgcgttta tactcggaat 3120 cttccagaga gccaagcccc ttgatatatc gaatggtatg cttcggaagt ttatccttag 3180 cttcttcata gtcaggaaga gaatagaacc attcttgttt atctttgagc tgcgcaataa 3240 taacaggagt tttcacaaag cgaattcgtc cttcatggaa cagttctggc cagttactga 3300 aaaacgccaa taatgacgga tagatactac cgacaccatc cacatccgca tctgtcatga 3360 tagcaatgtt tcggtaattc atattctcgg ccggttgtcc aatcgttaaa ccagtaatcg 3420 cacacacatc aaaggcttca gcgttcttca taacttcaac agctttcaaa ccccatgtgt 3480 ttttaaactt accacgtaat ggataagcac cttgcaattc acggtcacga accttgataa 3540 atggaccagt tgcagaatca ccttcggtca ggaacagagt cgtttccaac ttatcattgc 3600 cccattcgtt ggccttaata tgcttctgta ccttggcctt tgaagctttc ttatttgcct 3660 tagtctctgc tgctttctct gcagccaatt tacgggctaa tgcagcttca acaatcggca 3720 taatcagcgg ttcatttttc agaatagctt gcgcgatttt cttagcatca atcttaatgt 3780 gatttcgaat ttcaccagcc ggagaagtca atcgctcctt agtctgggaa tcaaatcgca 3840 tgttgctcat atcgcggatg aacataagca tggtgagaca ttctttaact cgtgccttgg 3900 tcacctcgat gcctttatat ttgcgcttaa tcgccggctg aaggtgttca cagatatcat 3960 ccataacaca ttcaacgtgg tgtccgccat tctttgtatg aatgttgttg acataagtca 4020 gctgacggaa accatctggg ctggtcgtaa aggccatcga tactgtatca gtttcctgga 4080 taacaacttc ggttccgaat tgcttggaga acttcttgaa attaccatca acctttttac 4140 cattaaaggt gaactgaata tccggataaa caaccgcgag agtctgcaga cggtccaatg 4200 taatatccag ataaatctgg gacagactat tttcttcaaa gtgactaaaa tctggaacaa 4260 atataacagt tgtacctttg cccttagatt tcttggtcga ccaacctttg ttttccatac 4320 cgttggagca gttaacggtg atttcatttg caccatcagc ggtaatccct gtgaacaata 4380 cggagaaaat attggtcaga gaagacccga cgccattcat cccgccagtt ttacgttcag 4440 catcatcgcc gaagttacca ccagcttttg gaatagtcca tgctgcaaca gggccaggga 4500 tttgttcacc gttttggtct gtaaccattg cttgaggaat accacgacca ttatcagaaa 4560 ccgaaatctg attatcttta atttgaacat caattttatt ggcaaattta aaactggtgc 4620 gaattgcttc atcaacagag ttatcgataa tttcatcaat cagttttaca ataccaggaa 4680 catagcttac ggacttatat tcaccaaaca agaatcgctc atgcgcttca tttgaactgg 4740 aaccaatata catcccggaa cgttttttga tatgctcgac gtccgagagt actttaattt 4800 cattattaat catcatcttt cctcttgtgt atggaggtat tataagtcac atattttaaa 4860 gcaaaaaggc ccgaaggcct tttatttgaa tcgaagctgc tctaacattt taatagcttg 4920 taggcgtccg gcttcagttt taaccgtaat aataacatca ccgccggtaa caacgatatc 4980 ctcttttgat tcctgttcgg ttatttcaga atcacattct ggaacttctt caaaatattt 5040 aaattcatca ccaaaaataa gaccggttcc attacgacta tggaaagaaa atccagtccc 5100 atcgatagta ctaaattccg taacaacatt tgaatccatc ttagtgactg aaaactcttt 5160 ggtggtaacc aaatcaatga tagaggttcc ttgttggttc aaaatactac caggcatacc 5220 gacatgttta aggaagccat ctaaatcttt aatgcaataa ctctttccaa ttttaaacat 5280 aatatcctca ttcaaatcgg atggttgaat ttttaaacag caatccagaa caaacagttc 5340 ctttaacttt cttaccggtt gggcctacgg ctgtaaaacc agtatgctga aaatcatctt 5400 cagagcaacc caaccagtta tatccggtga tttggatttg ctcatagccg tttgcatcta 5460 atacccgaat ggcattatct gcatcagtac aaccagccaa tgaaaccgcc agtaccagtg 5520 ctgcaataga acgattaatg tatttcataa ttcctcactt agtcagcagg tcgtaaaaac 5580 cgccattaac atgtttagga gccgagacta accgaacagc cagccgatgg caatcaggac 5640 aaacatcatt atctctttca gagattttct tgattttttc gtattctttc gcgcagtcat 5700 tggattggca tttgtagtca taaagtggca tgttattcct taaaatgtgc tttcaacatc 5760 tggtaaacgg tccaggcttc agagttatta gaaatggtga ttttagccaa agggaattcg 5820 cttggttcaa atgcttcatc agcgtcttcg cattcatctt cagtatcgtc atctttttct 5880 tcttcaaaga aacgacgctc atcttccttt atgaatattc tctccattcc accggtcgaa 5940 aattctatcc gatatacatc accaccttca attccgactg gtctaaatcc ttcaccattg 6000 attatagcat cagctatatt atcattatca gagcataaca aacggaatct gctttcagca 6060 acattatctt taaaataata ccatttatca gtttcaaatt tcaacatgat aagtctcctt 6120 ccaaaaatct gtaccatctt ctgattcttc gcggaacagc gcatagctag gtagttccgc 6180 accttcatac gcatacacat aaatgatatc agttgtgcaa agaaattcat tgatattttt 6240 cttaacaatc actttatcac tttcagcggt ttcccagaac tcttcagcgg tcagggaagt 6300 ggtcacacaa tcttcattaa acataatatt ctcctcattg atagggcaaa aaccctatca 6360 tttttataat ttaggcaaaa tgaatatcag catactcaac gataaagctc tcaggaacaa 6420 ccttaaaggt tccaaggaat acgatattat acagtttgac accttcttca acccactggt 6480 cggtgatgta accagacata ctagtggttt cataagtcgg accgtacttg caattgtatt 6540 caaacggttt aggtgaaacg aaaaagacac ctttacctaa tttccattta aaatcaaccc 6600 caacatgaga ctggtcaatg gtttcaactt cttcaactac tggagcgatg tatccagtaa 6660 attcagttga ttccaggaat ttaggatgta cataagccaa ttgagtttca gcatgaccat 6720 gtttaggaat aatacgcact ttaacttctt taattccacc tactaaatta gtgcgaagac 6780 tcacaacctc aacaaaacga ccagcagctt tagaacgaga ttctttagaa acacgtacaa 6840 tatcaccttc ttcaactttc ataattttct ctcttgatta ataagtttat tctccagtgg 6900 ggtcattata ctctgacccc aagagtttgt acactttaaa tttaattaaa aatttcaaca 6960 accatggaac gtgggacaac agtaaattca ccagcatgga caacgttcag gagttcaaca 7020 ccatcttcaa tccactggtc tgttacccaa ccacaaatgg aactgttaaa tgggcgtttg 7080 atttgaactg gtttacatag caagtcaata gggtcaccca gctcgctaac ttcttttagc 7140 agtacagatt cacgcggata aaggatagct tcaagattgt cacctgattc attgactgca 7200 gtaaattcaa agatgctttg gtgattgtgg tacttaactg ttttaacggt aaatggagca 7260 aatccaaaaa tacctttcag tacccgccaa ccagcatgca ttgcgacagt ctttccaaat 7320 tgactatcat cttcaataag ctgataggtt ttaccaactt tgataggagt ttcttcaact 7380 tctttgaaga gttcaatctc agcttcataa accataattt tttctacttc atttgtatca 7440 aggcgacgtg catgtagaat atatgcttta tcaaaccagt tactttttac aaaagtttca 7500 acaataaatt caccttcgcc taaagcatca gtcagagtat tatacccaga aaccatagct 7560 gcattttgaa taataggttc aatcagttga taagttttgt taatttcaat agtcatattt 7620 tgtttctcaa gttttaatgg tttaattggg cgaacataca tacttacaac atcaaagtca 7680 ataaattctg gtttcattat gcaaccttat tgaacatcgc cagttgatta ggttgtacaa 7740 ccataatttc acgacgacct tttgtatttg acacgttcag gtagatgcct ttgttcatgt 7800 ctgtttcttt atcatatacc gcaattttat tgacagtaaa gattttacca cgaatatttg 7860 caaatgcaca ttcaaacgct ttagtcagac ctgcttcagc agcttgaaga gcggacattt 7920 catattgttg gccaatttca aaagaagtaa tcattttgtt gtcctcatca gtagttgatg 7980 agatgattat gtctctttcc attgaccttg tacactactt tttatcgttt tttcggtaaa 8040 gataacgggt ctgcatcttc ttcggaagga acttcatcca aaccaagggc atagcgctgg 8100 gcatacttca acatcaaaat atctttcgcg cagtcatgta ccgaatcgtg cgccacgaat 8160 ccatctaaag tcccctttgg taatggagtt tcacacatct tacggacgag tgacaaggct 8220 tcgatggctg tcctgatatc tcgctggttc cagaatacaa ccggctccag gccataggta 8280 ttcaattcac gctcaggaat cccctcattg cgctgtaggt cgcggataag gtcaacaaac 8340 aacgggaagt caaaagacat tccccgacac cacataaggc tttcgtattt gttaattcca 8400 ttaccattca gatagtcaat cgcctgttgg ataccggtca gagtatcaac atcatgcgca 8460 gaagggacca ggttaacacg tgcttcggtt gattgttttt tccaccaacc catagttgtt 8520 gtgtcgaaca gccgcttacc tttttgatat tcaaggctga acttaatgcg tttgccgcga 8580 ttaaccagag tttcgaatga ttctacaata gcagggtcag attcaaatgt aatcagggcc 8640 aggtcaataa cggctccctt cgagccgttt cccagagttt caaagtcaat aatagcatca 8700 atcatcgcac atctctttta atttctgaat ttcgccagca acatagtcgg agataaaaat 8760 ccctggctct tcattgttaa aatattcaat cgcggccata cgtttccgaa taagattcag 8820 ttccagtaaa acgtaattac tctgcacgac gatagtcgga ttatcttcga aaaattcaac 8880 cgcgtcctga ataccataga tgagattatc taaatcttct ttatgtacct tatacataat 8940 atgcgtcgta cctcgctcgt gtgattccaa cataagccaa ctggctcgct aatgaagctt 9000 cggccatatg gatgcatgga gtatagatga atgctttgtc cactgaaacg ccctgggcct 9060 tgtggattgt actaaccgga agagcacgaa ctttcaagaa ggtccggcga gctttccaga 9120 agtctttcca ctctggacgt ttaccagcgg ctttcatctg tttgtatgtg tcagcaactt 9180 tagccagata tgcctggaat ttcggttgct ggtcttctgg taaaacttta atgcggtcaa 9240 cctgatattc ttcatcttca tcaattgact caacttccag gtcccagtaa ttaatcacta 9300 actggtgcga ttccccttta caacggagaa tggtggaagt gtatttgcaa tccttgatgc 9360 gaaccaactg accattatta aaaatggttt cgctgaattt cttaccttca aattccagct 9420 ctttcatgtg tggctcttgc ataaccagga cttcatacgg taaaaatgct ttatccgttt 9480 catagagctt gcgacgaata atattattca gtttttcaac tgatttattc gtgtacgcgt 9540 acatcctgtt ttccatcaac atatcagcat cttttacaat accgaaatag tttaccatga 9600 aatcctttaa agcggtttgt gaggtgaaac cctgtacgcc atggccatca tacatacagt 9660 cgcggaacca acccccatta cgaatttctg tcgccacttc aatgatagga gcattgcttc 9720 gcatgacctc agtcagatga atctgtttga acttaggatg cgtaaagaat gggcttatct 9780 tttgttgtgt gcttcctggg tcaactggct gcagctggtg catatcacca atcccaagaa 9840 tagtacacca ttcaggaaca gagttacaaa taatcttaaa cagagaccca tcatacatac 9900 ttgcttcatc acaaaccaga acattacatt ttgacatgtc aggcatttca cgctgttcaa 9960 aaatgttttg gtcttcatat gtggtcgggt taatcttcag gatactatgg attgtattcg 10020 ccgtttgacc agacaattta gatagaacct tttttgcctg gtgagttggt gcagcaagca 10080 caattcccat aactccatta cgcactaaat ggtcaatgac aaattttgtc agagtagttt 10140 tacctgtgcc agctgggcca ttcagagtga tacattcccc cgaacgtcgc tgaatagctt 10200 cagtgatata atcaaaagct tctttttgtc ctgtattcaa gtcttcaaat ttaatcatgc 10260 caattttact cgcttaacat ataaagtatc taaaactaat ttcaattctc gtgcgtgttc 10320 ttcagtagga aaaactgttt ttgtttcacg cttatcaaac caccattttc tgggaatata 10380 ccgtgtttca atacgtccca tgcgtaaata gcggtttgta gtatatgaat agttggcaag 10440 ttcatactct gcacccggtt ttgtggtgta taaggtaacc tttccatcat actccaatgg 10500 gcgttcaaaa ccatctttat ctttgtgaac tagcacatat gcgtagtaca tcacattaac 10560 ctctaatcag tttaaaaatt gacgccttca aaggcagacc acgtttcagt tttaatttac 10620 caataaaacc aatcttctca aaacgctcaa gaccttgaat gattttatca tagcaatcaa 10680 aaagcgtttc agcatcagtt aatttcttgg taactttcca gtcattacct ttgagcagga 10740 cgttcaattt agtaagctca tgaccttccg caacacggga accatcaatg cgagccttca 10800 gagcaaccat ttttaattgg ttttctttgg actgctctga tttactcatt ttgagcataa 10860 tgcttaacgc gattatcacg tttatgctgg atgttcttga tatgagtata cgacttttta 10920 atctgttccc atttctctgc agtcagattt aacttatcaa tcttcttggc ggatgggcgc 10980 gcaattttta cagcaacatc atcgcgacgt tgtttaagct catccaggga ccaatcagaa 11040 cgcatggtat tgtatttctt tttaacagga ataacattac ctttgatata accaatatca 11100 ttgttaaaac gctctaatga taacttctca ccttcaatag agttattaaa tgtctcacca 11160 gaataagcac aaactttttg gtcaagaatg ttaaccagat aattgaaatc caggttaaag 11220 tccttaccgc gacgtttagc ggaagcttga gtgtgttcca gacgacgttt aactttcagc 11280 gttttggttt ttgataaagt catgatattt ttctcatctt acggacgatt tgataactca 11340 gtatggattc attatacacc atccattggg tgtgtacact actttttgcc tttaacgctt 11400 ttatttttac tgtgtataat ggttctaagg tctttggcct tttgtcaacc cagaatactt 11460 cagtaagaaa gaacggaaag attccataat ttatagtctt cccatctata aatgctctca 11520 atagagtcca cgcgtagcgt agttcccgca gggaacatca tctcatcttc atcatttact 11580 aattgcaagt ttgaagaacg agttatttca ggatatagtc cattgaactc ttcaggaggt 11640 gccgcacaaa tcattttaaa aatcgcttcc tgataatcaa atgcgaatgg tgcatcatta 11700 atacagaaaa tatttttggt tccatagaag ctaaaggaag caaagttacg cgcaacactg 11760 aaatctttgg agaaactcat tacacggtca aactcaatcg tagcaccaac acatcctacg 11820 gtttcaagat atgccaaagt tttcttagaa atcccgcgat ataacttacc aggcacagca 11880 gaagtcaaat tcttacgaat aattttgttc aaagtttcct ggatagtgtc tggcttattt 11940 tccatgcact gccagagtgt actctgttcc aactcagagt aaatttcatc aatttcaatc 12000 tgggtatttt cacgaaaata ttcacattta actgcattga tgtactctgc taattggtga 12060 cttgcgtaca gcattttatt atcctcatca gtagttgata ggaacatatt aacgtgtctt 12120 attgctcttg tacacagtca agaacataat aatcaccaac aaatccagca tcaactatca 12180 cgaatttctg gtcaactgga acaaaccatt caagctcaga atcaatcatg tccatgtatt 12240 cttcacgtct taacgttgct gcctgttcgg aaccagaagc tgtattgaag aattccagtg 12300 gaacatcaag cgcgagctta cgcattgcat catggtaaca aaaagctttg gtctgattca 12360 atctgacgat agtcctagtc acatattcca ttgcagcaaa atccctggca ataccatagt 12420 cttctgtaaa ggaggttatc cttccaaatt caaaaggttt cattgattct tttgcttcat 12480 ctaacaaatc aatctcttgc tgggtaaccc cacgatacaa gactgtatca ggtacagata 12540 accttgactt gatgattgag ttaacgtttg aatgcaaggt cttatcgact tttgatgtgc 12600 ggcattcaaa taatgaagct atctccattt cactaaattt taactggttg ggttcggtat 12660 cagatttgat gatgttcata atggcctctc tgttgtaaga aagaccatta tgtcattagg 12720 aggggagtaa gtaaactact taaatactcc aagtgggacg ccaaagactg caatgtctgc 12780 ggccatgcca atcggagatt gatatgatga gtaagaactt ttcgattctg tcgggcgttc 12840 ggctttaatt gtcttggatt cagtcttaat cctttgtaag cgattaattt tttgttgctc 12900 aatttcctta tgagcgtgtt tacggtcgat atccagcgcc gatgtgggaa tatttgttaa 12960 ccagttcatc gtttcaccca tggtgcccat ggaggtgggt cgtcgtaata tgaggccagg 13020 tcgcgcagaa cttccattgg gagactaaaa ccatgcttct gtataatcgc atcaagttcg 13080 tcaactaggt ccacacgggc ttccgtagga gctttaggct cttcaaaaag ttcaaataaa 13140 tccattagta gccaccgata ggaccgtaac catacatacg agcacgcatt ttagtatctt 13200 gaatagcttc ttctgttaaa gtaaagcgca agccgatatc aaagtgtttc caaatctgac 13260 gttcaacttc agcaaggtcg tcggtagtgg aaatcagata tgtgcgacct ttaaggcgtt 13320 cagtggccat tttatctcta tctttataga aaatgatatc ttcgccataa aggtccgggt 13380 gacgatagcc tttgatatgc agagcttcgg aaacagtaaa cggacaccct tcagccacat 13440 taacgttttt gttatgaaat accttcatcg tgacattttc tggcatcgtc aggttcatta 13500 attttgtttc caactcagta accgaatcca tgccattgta aaccatgata agacaagctg 13560 tagcatcgta aagtcgtgtt tgtccggcag aatcacggac atagaattct aattcgtata 13620 ctttcataat gatttcctct taccaactaa tggaccatca ggaactttat tttccttgat 13680 gtacttttca ttttcttcca tcattttact gccaattttc aaaagcaaat ccagtgcttc 13740 ttttgcccgg cgttcagcct cttcttgtgt catgatttat ctccgtagat atcacgcatc 13800 agtttaagca cagctcgttc ggtcttcttt tcctgcatag ctttaagccc tgtagcagcc 13860 cattcttcag cttcgcgctg catttcttcc tcggctctgg ccacccagga atcatcaatc 13920 cctggagtaa ccaaccaatc tttaagccaa cttgtaattc tcatttaatc acacgagctg 13980 gaagacgaat cgcagcttcc tgaatcatag gaggaataag acgaatcacc agtccacgaa 14040 ctgacggcag cagcgactac aatcggagtc atatcaatat gcggtgtagt atcgacatgg 14100 cttgtagaac gtcttgaatc cagatgctct tttaactttt tagaattcaa tggtaatgca 14160 cctggtccga cattaccggc tttttctggt tggcgacgaa cttcttccat cgtgccatca 14220 cccatgtaaa catattctct tagcttgaca tctggtaggc taacaggaac cacaccttca 14280 ttcggtttgg tcttgaataa attcttgagc caactaatca taggaaacct cgtttaaatt 14340 caactttacc atcatcacaa cggatttcaa tattgaattc taaagaatca tcatcgtgca 14400 tcatgaacga gtgcacgaca acctcgtcaa acacacccag accatactta aaatgcacat 14460 catgggcaat aacttcatct aaagttttat ttggtttaat ccaacggttt aacatagtat 14520 tctcctctgt aattgaaata attataccgc ttctattaaa agcaaaaagg gaacccgaag 14580 gttccctata attaaactcc tggggtaggc gcaaacatcg ccgcgttctt agttcgccaa 14640 tcggctgcat cagtcactac agtagtatag gctgcaacgg ccgaaggaaa agtctgataa 14700 tgagcatccg caatacgatg ctcgttcgaa tagatttcaa acggaactga tacttcaaca 14760 cctttaactt ctttaccttc accagctgga tgcgtaaagg ttttgatatt aacaccaccc 14820 atgattaact cctggggtag gcgcaaacat cgccgcgttc ttagttcgcc aatcggctgc 14880 atcagtcact acagtagtat aggctgcaac ggccgaagga aaagtctgat aatgagcatc 14940 cgcaatacga tgctcgttcg aatagatttc aaacggaact gatacttcaa cacctttaac 15000 ttctttacct tcaccagctg gatgcgtaaa ggttttgata ttaacgaaac cacccatgat 15060 taactccttt gttgtttaat tacaggtgta tttataactt aataaccacg gtcctgacga 15120 gcaaagttct cagcattttt caggtaatac aatttaaaga tttcttctgc agatagacca 15180 agaccctgga acatattcag aacaaagtgc aggatatcaa tcatctcgaa tttaatttcg 15240 agctggtctt caggagataa attattaatc agggtttgct gcatttcgcc atgttgtgct 15300 ttccatggtt tccatacggc cgaggcagct ttctcgccat tggacatacc gccgagagaa 15360 gtcagaagtt cacggaattc gtcgtcaaca taatctttct ggttgcgaag ccatgcaaca 15420 acatcgcccg cggttgccaa ttcatctgga tgtgcgttat attctggttt ttctgtagcc 15480 agacgaacct gcaaagattt ctgcatatca agcataatct gcagagggtc atcattgcct 15540 tttaggacat cattatacgc atcttgagct ttatcaacac cagcaatcag ttgagaacac 15600 tcgttaaaat gagccattat atttttcctt atcaattcat gttgtttgta tgttaattat 15660 atcatattgg tttaaagcat aaatacaaga aaggaggatg tatgctcaaa ctatctagtc 15720 taattcaagc catcaaaggc agtcgtaagc gaacgattct ggtcctttcg atactctgtt 15780 ttgttctact ctggaatttc attgttgtcc ctattgcatt ggcacatggt gttatattgc 15840 cagtcgtcgc gctcgaatca gttgtagatg tttcactagc attaatcggc cttatatagg 15900 tggaacttac tggttgtgcc tatcgctgca acacaagggg tgcacttacc ccaggtcaca 15960 atggaacata ttcattccat catcggttac tttaccggaa tcccttccta aacttttgcg 16020 tacttcgaaa gtcgcattct cgccatcaat ccttgagcaa cgttatcggc catatatgat 16080 tcgatttgtt caggggttgc cccttctgat ttaaccatct catttatgtc tttcgtatgc 16140 catggtgcat tgtcccagaa cataacagct tcgccagctt caacgagctt cttcatacgt 16200 ttaatcgtat ccggatgtct tggctcgttg tccattaccc atactcttgt ttcagcataa 16260 ggaacagctt ccaatgccaa tgtgccaccg gttatcgcaa tgctgttagg gataaacaaa 16320 ctatcaattg gaccttccat tacccatacg cgtttacgct catcaatctt gtctactcca 16380 tagactttgg aagcttctgg gtgagctttg attgtgatat atttttgtgg tgcatctttg 16440 cgaagagctc gtccttggaa agattcgatt tcttttttgc cgttgaagat aggaataacc 16500 aagcgtggct cattcgtttc attcttatac gtcccgggat taacggagtt aactaatgca 16560 ggccatttgt ttgtaaacca taaccggttc caaacattgc taggaataca ccgacttttt 16620 acatacttaa ttatcggatg tgcttctggt aaagtatcga gtctttcaca aaaagcaagc 16680 tgctcgataa ccggagtttt ttctggttct ttcggagttt ctgcaacttc attgcgctgt 16740 ctgccaaagg cattctcctt atacttctca accatatatt cccggtgtaa atccggctca 16800 aactctttta tgtatttgct tatatgcgtg gagtagtcac agttaaaaca tttcacgtaa 16860 accgcgccat cagacggatt ggtatacgcc cagaatcgag ctttatgtga atctttctgg 16920 gagtcgccac aaactttaca tctgcagttt gccttaaatg tgctcccggt gacttctcta 16980 taacgtggtt gatacgataa agctctgact gcaaattctt tatcaatata tgacatagtt 17040 tcctcatttc aatggccccg aaggaccatt atactgtagg tttatcgtct ttgttcttac 17100 gcttttttga tgggatttgt tcagggcctt tattgacaac caccccggaa gtggtacctg 17160 ttgcggtatt agttgggttt ccacctgcat caccggcaac catatcttcg tttataaaat 17220 ctttgaatgt tttcataaca ccccctttcc gctatttata caacaaagat agctttatca 17280 ccatttgcat cagtcacttc ataagtgtct tcatgataca acggaataat ccaggtaaca 17340 ccatcatcag cgataacatg gattgcttct tggtcttgcg acacgatttc atattcttca 17400 aaaagctgga agaccgaaat cttagagccc gtacacattg cactcatatt gttcccttct 17460 tttaatcaat tgacgatgtg cctttttaag tttacgaatc tgacgtttag tcggtcgcgc 17520 aacgtaagct tgtgtagtta ctcgtccaaa aacaccatac ttaggcgaag gagtatcaat 17580 aaagacttcc cactggcaag agtaactata tattgcttta gagaattggg tcaaaacaaa 17640 catattaacc tcgcttcatg aaggcattaa aaatctggtc atcaatatca gtaggcggtt 17700 cttgagcttt gacccaagcc ccttccagaa tgtcatttgc ttcagtagat atcatgaccc 17760 caatttcagg gtttccggtt atccatactg cacaatcgcg gacattcttt ttatcaaatg 17820 gataaccacg attatcttca caaggcccat tcattgtcat aaatgaacga gcctttatga 17880 taacatcatc tttaacgata atataaccaa tcgcctgatt ataacgaggc tcacgaaatt 17940 catagtgagc attaccatta ttattgctca ccagttcaaa attcagaaaa ttataataac 18000 tcatattaat ctcgcttcat gaaggcatta aaaatttggt cgtcaactga atatacaggg 18060 gtttccagaa gtttcaaaag actgttttta atgtacttac ccgggatacc catattaaca 18120 atactgtcat tcaattcatt atcagggttg atgtctttaa attcaaaccc atcaaattca 18180 accttgtcag gaataccatc agcgaaaaaa taaaccaatt ggtgcattgt attgcgcgaa 18240 aataatttag acttgaccgc aataagttct tgccaatggt ttaattcttt gttaagaagt 18300 ttatgagcaa acgtaattct attatcacta tcaacaagaa aataacccag gtattgaatg 18360 ggttcgtcgc tccaaaacat cctgtatgct tgtgcatttt gatttttaca acgaagtttt 18420 ttgataacta gagttctgtt aatcatttta ttcacctgta acccattgaa caatcttgct 18480 gttgtcaaat gaagcatcgc aatacagaac atagttatac tgcataacat caccagaagt 18540 caattggtca gatacttctt taaccatatc aggacggtca cgtttagtaa tattcatgat 18600 gtcagcataa atctgatgtt caacatcagc ctggtcagca gtcatggacc actcgcaaag 18660 gtctttatca agtccagcag taactggagc tgtgccacat gatgctacgg taagaattgc 18720 tgcgattaat aactttttca tttcaatctc ctcagtagtt gatagggata atatactgca 18780 ttatccctat gttgtacact tatttttcgt tagaacgata atcatattca aaaactttat 18840 tgtcattaac aaaggcttta accagcgcat tattcacttc tgcatactgc actgtaaccc 18900 attcaatcat atttttgtca ctgacatcca gcgcggtttc tttggtaaac atattaccat 18960 tttcaaacca accaataaac actacgttct ctaatttata catttagatt tcctcacaaa 19020 acgcccatgc ggcgtgataa actgttgcgc ctttagtgtc atttaaacaa accacgtcaa 19080 caggtgcaat cacacgataa agacggggtt ctcctccata tgagcgagct gctcttcctg 19140 catagatacg cgccaaacca atatcttcag taaaaaacac acgccctaaa tttttcttac 19200 gcccaacttc tgacaaaacg ccagttgcat caggaggaca aagcatattt tggattccag 19260 cattagagct tgacccatga taataaactt tgtattctgc cttgcaatca atggttttca 19320 ttttattctc cagtagttga tagggtcatt ataacataag aaaaaggggc tgtaagcccc 19380 tttgttcact tacgccaact catagttgtg catagtcaga caacgaattt tcccagtagt 19440 tgggtgcata acagtcacac taattgtgta atcatcacac acgttaacaa aaggttcggc 19500 gataacatat actgccccac ctgccaacag gtattcacga agagagctgc gaacagatga 19560 aggttgaaca tcgcttttga gctttattaa tttaccagtt tgaatgcctg atgcgataag 19620 agcagcacag ttttcatcac ggcgagcgaa ttgtccaaaa tagtaacgat tcatgtcggt 19680 ttgattaaac atatggtttt ccttttaaca gttggtttat tgtgtaactc agtatggaat 19740 cattatgtcc taagacaagg ggcttgtaca ctattttaat tcattaatta aatgaaaatc 19800 ttgtgcattg aaagtaatga aaaaataatc attcccttca actgaagcat gacaggcaac 19860 actacaccag cgccttgctc tgttcaagga gcaatccaaa attttaaaag gcttcagacc 19920 aacaatggca gctgttcttt tattatgctc acaaatatca gcatactttt ggagagcttc 19980 ttcagatttg aactggtaat gcacattaac taataacatt ttattatcct catcagtagt 20040 tgatgggata atagtacacc ctttttatgg tgatgtaaac ctctaaaatg aaaaagaccg 20100 aggtctttag aatttcattt gagcagcaag gtcgtccagg tcgctcctgt tgaccctggt 20160 ttgtctattc atttcagcct ggcgattcat ctcaccggtg gcttctttaa ccgtagggcc 20220 ttgtttagga gctgaatcat catcaacctc gtaccagcgc tgattgcctt tcttaacacc 20280 catcttgaat ttattatagt agttcttatc gccataacga gacttaagct gtttgattaa 20340 ctgtagaccc atttgagcga attcttcggt ctcaacaaca cccaacataa agtcagccgt 20400 gtgagcaata ccaaaggatt ctgcgatgtc agccatatca atctcagctg caacgttcgc 20460 acccctggtt gtctgcgctg cagtccacag gaccacattt ttctcaacag ccagaccacg 20520 aagttcttct gcaaccatct taatcaggcc gtaactgttt tcggtaaacg ttttcagtct 20580 tgagcttgca cagattgcca ggtagtcaac aataatcaca tccggcacaa atccttgctt 20640 cagtttgtat tcattcagca atgcacggaa ggtatccgca tgagcaccgc cagttggata 20700 ttgttttatc ttcagagtcc caagagtccc ggaagcacgc catttttcca tcttagcttt 20760 atattccgcc catgatatat gaccttcatc aatatcatcc agagatacat ccagcaagtt 20820 ggcatcaata cgtttggcac atacttcttc agccatttcc atgcttatat aaagcacatt 20880 atgaccagtc tgaagatagt cagccgctaa cgaacaaagt ccaagggatt tgccgacgtt 20940 cgtcccggcc agtaaaacgt tctctgtacc gtactcaacc ccaccctttg tgattttatt 21000 caggatgtta atacggaacg gaaccttctt cgatttgttc agataagtct ggaatcgagc 21060 ttcgtaatcg tttaaccagt catggcccaa agcggagtca aaacagatag acaatgcgtc 21120 gcgcatgatg tcggggatag caccaatatc cgggattcgt ttatcacgtt tatctttcgg 21180 aagttcggca ttactctgga tttcgataat cctggatgtg gcattgaaca tcgcagactt 21240 ctgcacatac ttctcggttt ctttaaccaa ccattcctgg tcttctggag tatcggccag 21300 gactcctaag agtttctttg tgccttcata ttcgacttca gaaaggtcag aactgtcgag 21360 cgcgatatca agtgcagtct tagttggtac cgcttgatat tcttttacgt gttcgttaat 21420 cgtattaaaa agagtcttcg cgggaccctt ttcaaaatat gtgttattca gatacggcca 21480 gactttaaca aaatattcct tgttaaagag tagctgggat aaaatagttt ctaccacttc 21540 caccacctct ttttcatttt aattttatca atttcatctt gtatttgcat tgtaacacac 21600 ttttctacat gcacagcaag ctccgctttg cggtcttcag aaggggtgcc gaaatcaact 21660 gaaacttttc cagatggttc aattttaatt ccggttacgt acacaatgtg actggaccca 21720 tcagggagtg ataacagaat ttcctgccgt acagttccca tggattgccg gataatctcc 21780 aaactttttt cgtagtaagg tacagaagga gccttagctc cctccgcatc tttctcgaaa 21840 ttatctaaat cagataaatc gatatcattc atcaaggtct tccatcatat ctaaatcgtt 21900 ttcgatatct gccgcagacg gttttgtctt accttcagga gcagtgtatt tttcaacctt 21960 ggaggcaata agcgcatcaa cttcggcatc aacaacagca ttgctatcga ttgcacccag 22020 ttggtaatgg cgtttgattg catcacggaa aggttcgtgc ttgaacagcg ggccccagaa 22080 ttcggtgcag ttagtatctt ttgcgcgcca tgatttttct tcgcggacca tttcaccggt 22140 ctcaacatca agatattcac ggttatacca accaactttt ggtttcacca caaatccgat 22200 ttccattgcc atatcaagca ggccagaata cgggtcaata cccccatcga attttacatc 22260 aatgaagaat ttacttttct ctttgaccat acgagatttt tcggcgttca gaacaaattg 22320 gaagccttca agttctttac cagtttcttt aatctggcgt ttgccgataa tgaatacggt 22380 gtctgcggaa tacataacac ctgtaccacc ggtcataacg gttttgctat acatctcaat 22440 cgtctcgatt gtatggttaa ccgcgacaca aggaatatcc ttgatgctaa aatacggggt 22500 aacaatacgg aacagagatt tcagggattt agcacgagtc atatcaccaa caactttttc 22560 gttcatcgca tcttctgttt cttttttcga cgccatgtta ccgattgagt caataaagat 22620 aatgactttt tcaccacgct caattgcttc aagctggtta accatatcaa ctttcaattg 22680 ttcaaccgac tggattggtg tatggataac acgctccggg tcaactttca ttgcacgaag 22740 atatgcttct gtgataccaa attctgaatc atagaacaaa cagattgcat cggggtattt 22800 gttcatatat gcagagacca tcgttaaacc catattggat ttaaagtgtt ttgacggacc 22860 agcaaaaata gtcaaacctg actgcatacc accatctagt gcaccgctga ttgcaatgtt 22920 cagcatcggg atttttgtac gaactacatc tttatcatta aagaacttgg acttggtaag 22980 agtcgcagtc attttggatg tagaagcttt aatcaaacgg gatttcaaat ctgcaataga 23040 cattcatttt ttccataggc accattatat ttttctcaca ggtttaagat aggataatta 23100 tatcactgta tttttagagc aattaatgtt agttaatacc ttggcgtgct ttctctttag 23160 ctatacattt attaatatag ctcagacggt tttgagcaga acgctgatac atatcgcggt 23220 actcattagc ttttggagaa cccgtctccg ccgcctgata agcttccgcg cgcatgatgt 23280 ttatctgcat attgatgtat gacatgcaga cctgcgaaat ctggttaaca gtatccaggt 23340 cattttgtcg cttggtttct gcttccagct tggcaatgat ttcgtcatgc ttcgcagctt 23400 gctcgcgttt gtactcaatt tcttgttgag catatttcgc gcagttgttt ccacattgat 23460 tgttgtcgcg tttgtagata agtccagtac cacctttgaa atcaaaggaa ccattatgca 23520 cccgacggtc ggcagcaatt cgcgctttac caatctcgtt ggcattaaca acatcaccct 23580 ggtaagcaca accagatagc agcataacag ctgcgatagc taactttttc attgtatttt 23640 cctcaattaa aacattcata gcgggcttgg agccccaaaa atacccaacc caaaattaaa 23700 gttggaatca tatgtggagt cattattgcc cagtagattg acatactaaa aaatgcaatc 23760 agtacaataa ttgtaattat cttcattttg cttccttgat gataattact tgtgcgtttg 23820 atttacgttc agcaacgagt gcccagataa atgcaataaa ccatccgacc attgtccaac 23880 ctaacaggag gttaacaaag aagattccgc aattactttt ggttccacgc agtagcgcga 23940 taatccacgg cagaaaatat gccagtgcag ccaggaagaa agaacctgca ccaagaacaa 24000 cagtacccgc aattaatgtt tccattttaa tatccttaag ttattcatca gttgatgggg 24060 taatcatacg gcaatccatt gccgttgtaa acacttaaaa gtcaaacata tcaaaaagag 24120 ttgctttctt ttcgtaatca accttagctg cttcagagaa acctgttaat ggcttaacaa 24180 aagtcttctg gaacagtgtg ttgtagtcca tccactgcaa gacttggtca cgaatttcaa 24240 tcgggagttc aatccctgac ggccatgcaa tcacagtttc accaaacggg ttgccgactt 24300 taagaggaag tacatacacc ttttcccctt ccatgatttg cggaacagtc ttcagacctt 24360 tagttgcacg catatatgca agcacacctt tgatatgagc cggacactta aatccaggga 24420 aaccgccttc attatatttc tggatgttgt taaccgatga tacacctgcg atggtcatgt 24480 agttaagctc attaaattcc ttattgaatt ccttgaagta tgtctgcaga gattcttcac 24540 cttcttgcag catacggcgg atacattctt tcagagcttt ctgacatgcc tttggagtac 24600 tcgacttttg agtttccaga cccatgattt tcagtttcgg aaccgcatat cgtgtgcctt 24660 ccatatcgta cacgttcagc gcataacgct tcttcgccgt ccagaatcca cccacacctt 24720 tagaacccag cggaggacat gcaatcgcct cacggtccat aaacataagg tgctgtttgt 24780 tgttcatgta ttcagccagc tcacggaaag ctttatcgat tgccggttcc agacggtctt 24840 tcgcaaactt gtccatgaag tcaaccaggt ggtttgtatc acggaattta gattcgccac 24900 caaccttttt aatcaggtta tcgcactgga tataaatgga gtcagtatcg ccatagaaca 24960 cataagtttc accgcttgtc ccacaaacac tatcgaagta ttcgttaact ttacgctcaa 25020 tccactgcaa tgcaagttgt ccgaaagatg taatcgcggt tgcatttcgc aggtcaaagt 25080 aacggaacca gatattaccc agagcgccat aacaactgtt gataagcagt ttacggttaa 25140 tctgtgcggt catgcctgca acttcttcca tctgcgcttt gtacaacata ccattcagaa 25200 ctgtttgatt cagaccatgt aaggttgttt tcatattgtc atcaaagtca tgacgataat 25260 caacttcagg ctcggttgta ccagttcctg gattacgcag agcttcctta atcagttcac 25320 cattacgttg agccgcaagc atatagcctt tgtgctcttt acgctgatta aacaccttgg 25380 tgacttctgt cggaataata cccgcaacat ctttaaaata catcatacca tttggagcac 25440 aactaaatgt atcagatgga cggtcagcag ttcctgcgat gtattcgtac atcggcgctg 25500 gtgcaaatga cccggcaatc gtttctggac tgatgttaat ctgtcggatg atgctcggat 25560 acagagatgt taagtcacag ctcataacgt atttgtatgc gttcggaatc ggttccttaa 25620 caaatgcacc agggaacgat tggcgaacat gtgatttggt ttccggaatg actttgtttt 25680 gagtcttcag gctgttaaag ataatcgcat cccaagtttt gattgggctg aatacagact 25740 gaatctgcat ttttgcataa taacccattg actggctcag gttaataaac tgccgcttac 25800 ggtcaatctg cacaacacga taaacgtcga tgatgttata agaaatatac cgctggtgat 25860 ttgattcacg cagtttagaa atcgggccat catacgggag tttaccaaca cccagttcag 25920 attccgcaac ataatccaac gaataagacg gttggtttgt gaagctgaac tttttataca 25980 ggtccagata atccagaaca gagataccca gaatctgaat gatttcttgt tcttcaccaa 26040 agccgttgtt aatgacttta acttttgtac gacgatgtgg gctcagacgt tttgccgtag 26100 cttcaccaaa tacattttta aggcggttgt agatatatgg aatatcaaac ttctcgatgt 26160 tccaaccagt taacataacc ggagttttct cgttccagaa taccaaatac tgctggagca 26220 tttcttcttc tgaatcgaaa gccatataaa caattttttc caggatttct tccgggactt 26280 catcaccacc atcttcctgc agcttagcag caatatcaat agaccattgc tccacagtgg 26340 cgtatggtga gcccaaaagg tcaaagacat agaatttatc gtctaccgaa tcatagtgcg 26400 tcagggcatc aatagggtga cgtgcttcag acggttcagg aaacccatca ggagatgtca 26460 cctcgatatc gaagttagca acacggattt tacttgaatc atatttgatt tcgtgtgggt 26520 aacggtcgga caagtatgcg agtttaaagt cgtccatacc catagcttcg agacccatgt 26580 cattcattcg cttcatccac tgggatgcat cacgcatgga atcgaattct tttttcacac 26640 aacctttatt atagatgtct gtatactttg cagcttggtc ttcgcgggcg tgcataaaca 26700 agcatggagc ataagggatt tcacgaacac gttcatgacc attcttgtca atatatcgtt 26760 cgagcagatt gtcaccgatt tgttcgatag ttaaataaaa ttcttgcatt tggtttcctt 26820 tatagtcgag tgattgactt ttgttttatg acttgatgct attatactac aaaagggacc 26880 gaagtccctt tgctttaaat ttctattgta cgacgttcga tggtcggatt accattttca 26940 tctttagttc taaccatagc ctggaagact ctctgcccag gatgtaataa tttattgagt 27000 tccttttgaa attctatttg aagttcttcg gaaaggtcaa aaatagtttg agttggggaa 27060 agtccgtata cttctttaag gcgttcacat gtttcaattt catgcttcat atcaattacc 27120 aattgtatat ttggctacga gcttccagtc gtctttttgt ttgaaggaga taacacggaa 27180 gttgtttgtt actgcgaaca attcattatg ctcttcttct ttgatagcaa ttaagcccca 27240 gtcttcaagc agttgggcga tggaatcacg gcgctgatag tcttcgccgt caatatcaac 27300 ctgacgacca tccatgcgca gcatttcttt gaagtgaaca atataataat gaccttgctt 27360 ctggaggatg tggcagctct ggtacagcac acggtcttta ttatttgcga tacccatacg 27420 ggtcagagtt tctttaactt tcaggaagtc ttcaggttgc tttaatgtaa tttcaatcat 27480 tttaccattc caatatttgt tttttaattt ctttttgttc tttcacatta ctggtaacgg 27540 tctttaagaa ttcatccgtc accagacctt ttgtcttttt caaaacatct ttaagactac 27600 ctttgacttt caatgtttcc atatacatca aagcatcatc tgcattaatt gtataatatt 27660 tcatcaaaag ctttaacaca agctggacgt tgactacatc cggttcagcc ttcgcccatt 27720 tgccataacg cttgccttgt ggaaccgcat gcagcaaata attataatgc tgttggtctg 27780 ttaatccaga accgattaag ttcatcacgt tcacctgcat gatgcaatcg acatgttgac 27840 tcaaggcatt gtccagccag aatttattgt agttttccga ttgaatcagg ttccgtggtt 27900 ttttgctaac ggttatgtcg ttaagaatcg cgaacaattc attttctgct ttctctttgt 27960 atgaatcgga caattcctga attttgttaa agtcgcgtga tttccacgca acttcatgtt 28020 cgtttaattg aacgtcatca tcaaagagac ttacagccat ttcatttccc cagccagttg 28080 agcaaacaga taagtcaagt gaacttcggt attggccgca atgccatggt actgattatt 28140 ctcacctaca atctcataca tgcggatgat acttgcacca gtcagtttgc catacatttc 28200 atcagccagc tttgcaatga agcttgaata atcatttgca tgcttaggag ccagaccacg 28260 aagaacttta aagttacggt ccttaagggc atcaatcaca tcatcaattg gcgtgcgatt 28320 gtttgtcacg atactcagaa taccagaatc gataacaccc tttgcagagt aacggtcaag 28380 ctcgttaatt gtcttacgca ggtccgggaa gttatgctta accagagcag caacaacttt 28440 caggtcttcg atttcaacgc cttcattttt actatatcgc aactgcacga tgaatcattt 28500 ctttcatcat ggtagtctgg tcagccgggg ttgattcgcc gaacttaatg acacgacaac 28560 gagactgcaa cggagtaatg atgccatcga tattgttcgc ggtcataatg attgaacagt 28620 tgcttgaata agcttccata aagctacgga gatgtcgttg tgattcagcc agacctgcac 28680 ggtcaaactc atcgataacg attaccttgc ggcgaccttc gattgatttg gatgatgcaa 28740 agcgagtcaa ttcattacgc acaaagtcaa ttcggcaatc ggagccgtta acaaacagca 28800 tatcagaatt cgtatctgcg cacatagctt ttgcaactgt tgtcttacca gtaccagggg 28860 aactactgac cagaatcaag ttaggaatca aacctttttt aacaatcgcc tctaatgttt 28920 ctttatcatg agccgggaga atacactctg ataatgtacc tgggcggtat ttttgttccc 28980 acatgaagtc ggattcgttg actgtcaaac cgttaattac tgttgtcatt attattttcc 29040 tcatattagt ttctactttg tactccatgg ggccgaagcc ccatttatta atcttggaat 29100 tcgtggctag agccttgttc cattgcaatc acatagcttg cttgtgtacc ttcaaacttc 29160 gtagcaaatt tatcatcttt cgcccaaagc ataacacggt aatcagccgg aatcattttc 29220 atattcagtt tgttaatgat aaatttgaac gctgcatcgc cttcgtaatc gccacacacc 29280 agggaataca atggacgcgc caattcttta tcttcgagct ggttataacc atggataaca 29340 atcttaccgt ctttgttggt gattgcaaaa gtatccagag acaaaccacc agccacacgc 29400 agaagctgct gcaggtcact gcctttcagg tcgaagataa ccttagctac cgggaaagga 29460 atcttcttag cagggaacac gatagtgctc gggtcagccg caggccagtg aatcgttgaa 29520 cgttggtctt tgataatgat atttgatgcg tcttcagcca gagatacttc agcttggtca 29580 gttaccaggc ccaggatact taagaagccg ttcaggtcat aaatcgcagc ttcaaagtca 29640 attgtatcat tgatttcgga ttcgccatat gacgctccag ttacagaacg agtcaggatg 29700 gtattgcctg gtttaagcat aatgccagag tagatggagg tgaagttttt caaaactgcg 29760 atagtgtctt tagagaattt catttaatta ccttttattc aaatcaaaat taattaagca 29820 acaactgcca tttgtttgcg gatagcttct ggaagagatt caatatactc tggaagttgc 29880 gcagcaataa tagaaccagc ttgcaccaga tggtcgtcag agttaacaca gtaatcatct 29940 tcaagacgtt cggaaccgtc acggtcgata accagtttag caccagcagc cagtgaatcg 30000 ttgtaatgac gttgcatcag ttcaatccac ttatcagata catcagtatc gataacacca 30060 tacaacatga atttaacttc ttgcggcaga gactgcagac gggtggcaga tacttcatca 30120 cacgccagga cccaggattt gcggatgcgg ttttggttat gtgggttggc gtcggtgcgg 30180 atggttttaa cttggatatc tttagcagtt acgttgttca taatattctc tctattcatt 30240 atcaattaat caaagtattc atatgcgaac caggctttgt gtctgttcgc gattatttta 30300 acatattctt caccgaaagc gttttgtaag tcacgttcag taacaaggtg ttcatctttc 30360 gaaaaggtat taactaacac ataccgttta atttcaggga cagccagtaa atgctgcccg 30420 gttaagaagt cagccataat agcctctatt ataacatcct tgtttaaagc attattccat 30480 aacagtgaat cgaccaactt ttttcatctg gagatgttgc ccgtatgctt ccgggtcatg 30540 gtctcggtga ctgataataa agatgttggt gtcttccaat ttgttcagga tggtagcaat 30600 tgctttaaca ccctctacat cggtggccga gtcaaaaact tcatccagaa tcaaggtgtt 30660 aatattaaca ccagatactt tcgcagcaat atcacgccat gtaaacagca atgcaatatc 30720 gatacgagct ttttcaccct ggctaaatga tgcatagctg aagtcttcac gtccacgaga 30780 tttaatcgtt tcgttgaact cttcatctaa cgtaaaacta taatcagctt ccataacttt 30840 aaggtaagcg tttatctgct tattaaagat aggaatatat ttcttaataa tcgcaccttt 30900 gataccggaa tctttcaaca tatccgttag gatgccacgt tgatattttt ccatcacaag 30960 gtcagatttc tgtttgacta ttttatcaag ctggtcttga agcattttaa tgtctgctgc 31020 atggtcgatg aattctgact tagcttgctc gattaatgct ttaacctttt tagctttttc 31080 gacagtctgg actaaggttt gtttcttagt ttgaatgtct tgagctattg tacgttgttt 31140 ttgtcttgca gcagaatacg catcaaattg ctcctgaaca gcgttctggc gagctttatg 31200 gttatcagct gatacattac attcttcaat ctttgactca atcctggcga caatggggtc 31260 tcctggatgc aactgactgg cacaagtctg acagcaacca cctttctcgt acagaccaac 31320 aacctttcgg taatcgtcta tcttcgactt aatcaggaat gattcctgct ttaacttcgt 31380 gagttcatgc tcagggtctt ctcccaagat tatctcagtc agtcttgtcg ttgcttcttc 31440 gatagtcgtt ttgattccac gtgcttctgt ggcaagttca tcatacatcg tttgcagccg 31500 caacagatta tcacctgata attttctttg acggtcttcg ttgtcctgat agattttaat 31560 ctgttgaaca atcccgtcat gctgcacatc catcacctgg atttgactat tgacttcgcg 31620 gataagagat ttgttcagct tatccatatc ggccagtaca gaaacctcta aaaggtcttc 31680 gacaagtttt cttcttgcag gagtggatag gcccataaaa ggggtatatc cagctgtgcc 31740 gagtacgaca atctgtttga agctcgcata ggacattccg atgagctgtt caaattccgc 31800 ttggaagtct ttactgctgg cagattcatc aagacgttta ccgccacaag agatttcaaa 31860 aacattgggt ttttgcccgc gtttgatata gtattctttt ccatcatatt ccatccacaa 31920 ttcaacgagc atatcttttt tgttgctcga gttaatcagc tggcctttct ttacatcacg 31980 aaaaggctta ccgaacatag caaaggtaac cgcttcaagg aacgtagatt tacccgcgcc 32040 attcttaccg gtgaccagag ttttttggac tttatccagt tcaacagtaa tcggttgagc 32100 accaaccgac ataatatttt tatagataac tttcttaagc ttaaaagtct tcatcgtaat 32160 aagcatcctt gatacatgat tcagccatat ggattaatgt ctctggagta tcatcttcca 32220 ttacattaaa gctaaattcc acggcccaat cggtgactac atagactttg ataagttgac 32280 cagtttcccg gtcagtgata gcttcaaacc aatacttaat agtattcggt tgattatcgt 32340 cgtcaaccac atcacattcg cggatatgct caagccccgc ttcattaaaa tcataaagtc 32400 tcatggttgc acctcaagat ataatttgtt ggccaatgct ttgagagctt ttgattcttt 32460 ctcattggcg tcaggtaatg catcgatata ttctgccatc atatctgtta agcgttttat 32520 gtctaactct tcatcagagt ctgacgtatc aacactgtta tcaaccttat tgacaacacg 32580 caattcgtgc acacgctttt ctaattcaga ttcgaacttg gtcaggtctt tatcgacttt 32640 ttctacaata acacggaccg ataagtcttt gaagtcgtcg tagttaatag tttcgtttgg 32700 atagaaaatc ttccgatgcc aggtggtttc attcggaata aatttcatct cttcggtttc 32760 ggtatcaaat tcccagaacc cgcgtgggtc gttctcatca cctgctgtta atgtccatgg 32820 tgttcctatg tacttaacgt ttgcagcttc tgaaatggta tggaagtgac cagaccaaac 32880 ttgcttgtac ttcttaagga aatcaggctc aagtccgtgg gatttcaatc ctttatagaa 32940 ataaaacccg gttaattccc agtgaccgat gcaatattca gcaccagaag ttttgatatg 33000 ttggtgaatg tcagttgcgt tctcgtcaca catccatgga atcatatcaa tctgacaacc 33060 gtcaaaatca acagtcgttg gcttatcgta gattttaaca tggtcatact tattcaggag 33120 ttcagtgata gcattaggat gaatcttatt cttcagattc atgtcatggt tcccgactat 33180 cgtgtgcata gtaattcctg cttcacgaag gagttccata atctcacggc tgaattccat 33240 cgtcttatgc gttacggcct tacggacgtc aaaaatatcg ccatattgaa tccagacttt 33300 aatcccattc gctttggaat atgcaatcgc ctgtaaaata ccatcgcgtt gaatgttttg 33360 agcccatggg tcgtcagctt tgacacctaa gtgccaatca cctaaattta aaattttcat 33420 aagccaagca ccgttatcgc aatgcaaaat aaaattattg aaatgaaccc gtcaggagtg 33480 gaaaagaatc ctatccaaca agctcgtgtg aatgtataga atgccatgaa tagcacgaca 33540 tatcctaaaa ataattccat atgttctcct cagttggttc attttatcat cccgaatata 33600 aagcaaaaaa ggaagcacta ggcttccttt ccaattaata caccgtacag aggttcgtga 33660 gaagacctga acttgatagg cttataggta atagtacctt cattttctgc tttaatgcga 33720 gcggcttcaa attgaagtat catctcattt ttgtcagatt taatcttatc aaaatccgtc 33780 gaattcgtca ttcatacagc ctcttaaaaa tcaggatgtt cgtcttgaaa tatattaccc 33840 aatcgtttta attcatcaga agtaagaata tctacatcat caatagccat aatatgcctc 33900 ttaaactttt tcgtattcgt ctttcattag ccaacctgtc catgttatca tctctgccac 33960 cacttcttaa gttgatgaga atcaacccaa accttggcta caggctcaat cttaaaaata 34020 ccatcagaat attggcccat cattttgtat tcggtttcgg tctgcttaat acagatatta 34080 ccgtatgagc gaagtctgaa agcggctcca ataagaagcc gtttaaaagg tttatttact 34140 aatgccattt acgatttccc aaatttgttt gcgagtagaa tcccacatca acttaatcag 34200 gtcttccgta acgggttggc ggtaaatccc acgcagttta actaaagcgt atttgtatgc 34260 ttcaaaattg ttttccagca ctgcgccttg agcatgctta ttcagtcgtg caatttcacg 34320 agcattcttt ttcagaatct tcgctgcttg actggttgct tcaagtgcgt tcgcttcttc 34380 gagacgttcc tgtgcttctt caatctgctt atcggtcagt tgagaaatat ctggagcttg 34440 catattaatc cttgggagtt aactcaattg taaaatatag attagcatta tccacatgat 34500 aacggtaatc aacgttatta tccggtgaag taaattccag attccggacg tttaaagggt 34560 cgatatcaat tggaagacct tttatgtctc gcagataaag tttaatgaaa tatgggagcg 34620 cagtaaagtc ttcctcgatt ttatctaaaa aatcggtaat atcaaattga tgtttcatat 34680 aaaaaatcca aatcaccttc tttatctttg gcgctcttat cagaccctgg cgctttaact 34740 aaagaggatt cgtactgtgt cattttgtca tagatgtcct ggataaatgt ttcatcagcc 34800 agagccacca tatcgtcatc acgtgaatcg tatacgttat ggacgaagta gctatatttc 34860 tttgccattt ctttacgttc ttttttgata cgctgaacga aagcattgaa gcaagccatt 34920 gttatgtaag catgtggatt atcgtatttt gtttcgtcga agttgtgaag accttttatt 34980 gcggcctcaa ttccgtctgc tatcatttct tgcttccagg attgggtata tccggagaag 35040 ttgaaacgtt tgctaagacc ttcggctata agcataatcg ccagaccaat aacatcgttc 35100 tggcgaataa ttttgttcgg gtcggtgttc gcattcagct tctgtttcca ctccgaaata 35160 gctttcaaaa gctctttatt atttacatag ttattcttgg ttaatttaat ttcgttcata 35220 tcgcctcact tagtatatga atattatatc atccttaata caaagcatta gcgaagtaaa 35280 cagtacgcct gaaagacctt ctccataatt gccttaaatt catggatgtc aatataaggg 35340 ataacctcgg catcactaat gaattccatc agacggtcat gctcgttgcg gtattgtcct 35400 tcattcaaca tgccgatatg ggactcccac gagccatacc cttctacgtg tgggaagaat 35460 gttattttca tgaattcatc cacccaaaac gttcccaggt cctggatgga tatcattgta 35520 tgattgatgt gcatcagtac accaattgag cagcttctaa aacatcatca agcttatcaa 35580 actggtcaat ataatcaatc ccaccaactt catctttagc atacaaccaa tattggtgga 35640 attcatattc aataataaag tggtcacctt caatctggag aaccccatca ccattaaatt 35700 gaactgcata acccgtaaag cggacatcat tcaggagttc ttctttcata gtaatcattt 35760 ccaagttttt atcgtaatta gagagtttta ttttaatcat taagataatc ctcaaattca 35820 tttaatgtca taccttcgtt ccatgctgtt ttcaggtgcg gcattctgtt atacgtcggc 35880 tccgagctta taggaaatat cctgaaatcg cgattacttc caccaagcat attggcagct 35940 tttataatag tttcactttt attcattata gttgtacgtt gaacaccatg aataaatgga 36000 cctttaaaaa caattatggc tgatacacct tctggtaaat tattcatttc gcacccttaa 36060 taattttcag gtcaacaaaa cctttcttgc gctgggaatt catacgcttg atagctttat 36120 cagaaacttc tttgttttca cgcggaagcg gcagaccaaa cgctactaca tcaaattcat 36180 tcaggatata ctggacaagg ctttcacgaa tacgcgcttt acccattgtc tgaccttctg 36240 caataatcaa cttctgtaca tcgccttccc actggtccag aatctcaggg gtaacgaaac 36300 cagatttcat cagagtgtta actttagcaa atgcgaaatt agtgatgatg ttagtgatag 36360 atgcagtcat gatatattcc tcagtctcat taaattaaaa ttttattatc ctagggtcat 36420 tataccatga ccccgttggt ttttactgcg ctttagcgcc tttccagcct tcgtacatca 36480 ttgcaacgga taaaagcaat gcgccttcgc atgcagcata cttattattc caaaaccagt 36540 tcagaaaaac ttcatcctca atgccagagt gtttcatgtt ctggaaaaat ttggcacgtt 36600 cttctgcaat acgttgtgac aatttagatt gtggattcat tttaaatttt ccagttaccg 36660 ttttcatcaa taaattcaat ccagtcattt actgaccact tggttgtatc gccttttgga 36720 gcagaattta aagcgtataa tccttgttta aaaagcattc gtttgatatt catgttttcc 36780 tcagctgtaa cgatagcact cgtttgattt acgttttgca acacggtgag aagtattata 36840 atcaaaatca tcctcaacgt atacatcttt tttcaactga cgaacttctt tacgcagttg 36900 acgattcatc tcattcttga cttctgaatc aacgccacgc atacgcttcc atttgtctga 36960 acggaagata ttttcttcaa cttgttcctg acttacacca tcaggcgcct gacgacgccc 37020 tgaataataa aaatctttta cagacaattc tttgcgacga atagttttac ccatagttac 37080 ctcagcagtt tgtatccaat aagcttttta atgtactgga tatctttgac atccaaatcc 37140 aacttaaggt ctttcagacg gtcacccagg gtcaaaaagg ttacatgttt ccaattcttc 37200 actgagcaac aataagtctt aggcccattt gcaatttcaa tatttttatc tgtgacggtg 37260 ataaaaatca aacgttcatc atcaagatta agggtggcga catttttcat tgaatgtaat 37320 ttcaggctca taatttaggt ccttttgcaa ctgtattgaa caagctacgg tcacacacca 37380 tcaacggagc atcaggccat ttcaaaggac gataccatac atcttggtcc gtcactttgg 37440 ttacttgcac aggatagcca tttactgaat aatactggtc ggctttaatt tctttatctt 37500 caataaccat tttgttttcc tcatcagtag ttgatgggat aatgatacac cgttcaaagg 37560 ggcttgtaca ccccttttta atcttcaatt aagtcctggt cataccccag ggatatcagt 37620 tttgttttaa aggcaggaat atttgtcatt ttttcatcat tgacaaaaat cactgggtaa 37680 cgaatgttaa gggtaggata tccagcacgc ttagccaaag acacgattaa tggcctgtca 37740 taagcgattt cagagccttg tttgttaagg actgaataga atgtataagg aaggtttaag 37800 gagcccagaa ggctcctgac gtgcatacaa cctgggcatc gaccgacttc ttcggggata 37860 ccatagattt caatcttagt cgagttttgg tccacaggaa atcactcctt ggtagttcca 37920 ggttttaata acttcttcat cgctggcagt tgcgtacggg acataaacct gaataaggaa 37980 acccgtgtca tcgaaagaga aataaaccgt cggtttgatt ccatgaatct cagacaatgc 38040 atagatgtct ttaaggtctt cttccttacc gacaaagaat tccgggtcca ggtcacagtc 38100 gtggaagtac cattcatcac cgaacttgtc ccatgcggtc caattagtag ctacatattc 38160 ttcaaaacgt ttcattcttt caccttcagc atttttgcaa tggtactatg aatagtctgg 38220 attcttttgt tgaattcaga gctttcgagg ccaggattgg tgcttaacca accgataata 38280 acaaacagca aaccaaacgg gatagccaga ataagcagac aaacgatgaa caacacaagc 38340 gttatgtttg ccagaatatc tgctgtagca ttacgaaatt tattcatatt tacccttaat 38400 tgattaacca agcattgata aacactaaac tatactgcga ataaaattct ggaccaaaac 38460 gaaaaatcat atcatttata gtatccataa tgtaattcaa tttaatcatg tttccacacc 38520 ccatcggtat ttgaccaaag tcgctgatta tctgaccctc gccacagttt tttggtcgga 38580 agatttttct catacttccc atcaataata acatcaacat atttaagcat ttctagttgt 38640 ttaatatctt caaacttata tcccgtccac atccaaatgc ttttattggg ataaagattt 38700 ttaatagttt gaaccacaga gtgaataaca tctcggttat caggatagag agggtctcct 38760 ccggttatag ttaatccttc tatataatca ttattcaaac attcaattaa ttgttctagt 38820 gttttaccag taaatggaac accatttcta gcattccatg ttgatttatt ataacaccct 38880 tcgcatttat gcaaacaacc tgtaacgaaa agaacgaccc tacaaccagg gccattcaca 38940 aaatcacaag gataaattct atcataattc attgatgttt aaccctatgc atgatttctt 39000 tatttttgcc gagattaaat ccacgttcgt tcggattccc caaatagccg catgttcttc 39060 ttattgtgtt catcttttta gggtcagttt ctccacaaat agaacaaaca aatccgtttt 39120 cagtaggagt catttcatgg gtacttccac acgtaaaaca tttatctact ggcatattaa 39180 caccaaaata atctaaatgt tgtgcagcat aatcccatac agcctcaaga ccttttaagt 39240 tatttttcat atcaggaagt tcaacataag aaatgtgacc acctgttgca atgaaatgat 39300 acggtgcttc gcgagaaatc ttttcaaacg gagtaatatt ttcttctact gaaacgtgga 39360 aactattagt gtaccagcct ttatcagtaa catcttttac acttccatat ttttcggtat 39420 caagtttaca gaagcgatag cacaggtttt cagcaggagt agaatacaaa ctaaaagcaa 39480 atccggttct ttcagtccac tgcttaagac gagcattcat tttagtcaaa atttcttgtc 39540 caatatcacg accgacaaga atattcaatt cgtgaatacc aatgtatcct aaggacactg 39600 aacttctacc gtttttaaat aactcaatta tatcgtcatc gggtttaaga cgaaccccga 39660 atgcaccttc ttgataaaga ataggagcaa cagtcgcttt aactcctttt aaggaactaa 39720 ttctacacat caaagcttca aaacataaat ccattcgctc attgaacaat tcagtaaatt 39780 tctgttcatt gaactgtgtt ccaatataag aatctaatgc aatgcgagga agattcagtg 39840 ttacaacacc aagattatta cgtccatcaa gaatttcatt accagtcgaa tctttccata 39900 cgcccaagaa actacgacaa cccattggag aaacaggaat agatgaacca gtgatagctt 39960 tgttattctt agctgaaatg atatcaggat acattctttt gcttgcacac tctaaagcaa 40020 gctgcttaat atcatagttt gggtcgtctt tataaagatt aacaccttct tcaacgaaca 40080 taacaagttt agggaaaata ggagttatcc catcacgacc aagaccttta atacgatttt 40140 tcagaattgc tttctgaatc attcgttcgg tccagtcagt tccggtacca aatgtaattg 40200 tcacaaaagg agtctgacca tttgagctaa agagagtatt tacttcatat tcataagctt 40260 ggaatgcatc gtatacgtct ttttctgttt tagattgggc ataattcaac gcatcagtga 40320 tttgccattt ttccgcgtcc tcaatatgtt tcgcataggt acgtttaaca taaggagaaa 40380 gtactttatc tacattcgca aaagtcgttc cgccatattg atgggaagca acctgcgcag 40440 taatttgtgc cataattgca gtagcaacac tgattgattt aggagtttca atctgtgcat 40500 taccgagttt aaatccgttc tcaagcattc cttttaaatc tactaaacag caattagtaa 40560 atggaagagc aggggaataa tcaatatcat ggaaatgaat aattccgctt tcatgtgctt 40620 tcataataaa agacgcgacc atatttttgg caatatgttt agatacaata ccagccataa 40680 ggtcacgttg agttggaaaa acacgagaat ctttattagc attctcgttt aaaaggtctt 40740 tattagtttt atgaatcaat ccttcaattt cgttttcaat tgtcatttta aactctttct 40800 aagctgcttc ttaaatgaag ccattaattg tgttttggtg tcagattcat tataagtcat 40860 accattcaaa agcatttccc gaatcatttc ttcttttccc aggcgaccga actctttggc 40920 tttgtctgtt acaaaattgg ggtgaatatt gttttcggta taatcagatt tcagatagac 40980 aagcaaactc tctaaccatt ccaggtagtc aacatcgcga ctttttagac ccgaacggct 41040 gaacttatgc ttcatttgtc cttctgttcc attgcacaga ttgcatagta acccgcgaac 41100 cttaccggct tttggtccgt tcaattcgtg gtcatggtca agatggttgg cctgaacatc 41160 agagtccaaa tcacggtggc aaattggaca cttaccagct tgtgcatcat aaagtttttg 41220 tttttcttct ttgtataatt tgccagtcaa caacataata aaacccttac cattattaga 41280 taagggtatt tattaatatc ctaatagttt agcagtgcaa cttagcacta acaacaatcc 41340 gaataaagca actccggccc aaaataccgt aataagagca tccatatcag accccgtaga 41400 acgagcgatg caaacgttta aattgacgaa tcatcttttt agtaggacgg gtatataatc 41460 ctacacaggt ggccataaac tggtcagtag ggcttccttt gataagaagt ttccagacct 41520 tgtcaccgtt taacttttcg gtatagacta atgcagtttt cattaaacaa tcctaaaaca 41580 aggataatgg cattcaacgt taccattatc aattatcata tactcagtga tacaggtcca 41640 tgacccttca atttctatgg tatcaaaaag gtattcatct gtttcaacaa cgtcccaaac 41700 attacattca acgtcagggt caaaacgttg tcttaacgca tggataacat caccattttt 41760 aaatcctgcg tcttgaagct ctttcataaa acctgattga gcagcatgaa tattaacaat 41820 ttctaaaaac attaaaagac ctcatcagaa aagcggagtt gggcttcaag ccaatgaatg 41880 tagtcagctg cagcaaaaat taaagcagat tcttcatcgg gtaacttacg agaatctgac 41940 aattcgtaaa gcgaatgctc aatcagacgg cctttatggc ggccaacttc aattttggta 42000 ctcatatcca aaccccatat cgattaaatc attgtttgaa ggttcattat aatctatttc 42060 ggaatcgttg taaacaggtt cctctggctc gacagtttcc catcgagcgg tataccatgg 42120 ttttatagcg taatccattc tctgatagtt tgggttacga cacgttcttg gagcttaacc 42180 agttcaacag tcgggaccat atggtcccag tcagtatgat aagagccggt acgagtttcg 42240 tttacagcca catgcacatt atgttttttg ctgaaataaa ttgcctggcg atattggtat 42300 ttgccggttt gtgtccattc gcctaattca accggaacca ggtagtccga aaattcaaac 42360 tcgtatgatt cagtaaacgc atcatggtct tcaatgattt cgcttaaaac agcatcaaca 42420 tctaattcat tttcaatagt cattggtcac tccaatagct tgatacttca aaatcaggct 42480 cgccgttttc ccagtaccaa ggaccaaccg gatgccaatc accatgctta tcttcttcat 42540 attcttcaga ttcaagccca acgtattgcc attggacttc ataagcagtg ccgccgatta 42600 caacagtgtt ctcaccatat ggattagcca cagcaaagtc aataccttct gctttagcca 42660 gccaaaccat atattcgtat agcttatatt ccaggtcatc ttcggtacca tcacctagaa 42720 aaatggtttg ttcgtttaat tcaatcataa ttccaccata aattcaaata gctggtcacg 42780 tacacaagta tcagtccaac cccattcgtt ggcttcttct tttaaccaat ccgggagatt 42840 tgccaatacg gtttctttac taggcgcagg agtatcaaac tctcttgcgc aggtttcata 42900 agctttatca aattcggtca tttaaagttc cagaatgaag caccaaccag aaataaaggc 42960 caacaaatca tagacgtcag ccaccagcaa aagtcacctg caccgaaatc atctaattta 43020 gctagggtct tagcataacc aataccgatg ataagataca aaacacccaa caataattca 43080 atcatttaaa ataagctcgc agttggtcga agccaccaat atgacttcca tcaggggcaa 43140 atacctgtgg catggtcaaa ccaatttgag attcacgacc aagcgcaacc aggagttgag 43200 caatcttctc atcatcaaac acaccttttt caggcataac gttaatgaat tcgtaaggtt 43260 gttttttaac gtcaagtaaa cgttttgcat tgtcacagaa aacgcaacga tgaatgtttg 43320 aatcgtagcc atatacttta aacatattat tccttaattc ctaatacttg tttgaaggtc 43380 tcagtataat cggagtattg accggtttgt tctttatgtt tgaccataat accataaact 43440 tctgaacgca tattattata tgaacgagtt aaagcagatt tcaatactga ataactatcc 43500 ggaagtttac cgtgttcttt ataataggcc agagttaatt cacggaccgc cttatcagca 43560 gtctcaagat attctttacg ctttgccatt ttcttctcgc tcaaatttgt gtttgcaatg 43620 tctgcattta taacgaagat tactagtctg ccaatggact aactggacct gttcagttcc 43680 acattctgga cagtttggaa cgctcttaga agcacgttcc cggcggcgaa ccatagccat 43740 taccatatcc caatcaggaa cctgactata atcatcagtc cagtttataa gacctttatt 43800 aacccccgct gcgggggtcc attctggaaa tgcagtttct gcaatatctt cagctaaacg 43860 ttgtttcatt tcagcagctc cagagcctgt tccagtttat ccagacgatt agttgattct 43920 tcccaaagtc gcttggcttt ggtatagtct tcagttgcct ttttattaac agcaagagct 43980 tcattatacg ccgtttccag ggcaacaatt tcaggcgctt tttgtttcac cggctcgttg 44040 ttcagattat caatcaggtc ttcaagggtg gtttcttgtg gtgcattcat aatttcctca 44100 cttttctttt tataaatttc tgcaacagtt ggttgttttg cacatttaac ttcgtgtaat 44160 gtcaccacaa ttttccaacc tttccaacca tgagcactgg tgatagggtc tactttaatt 44220 ttttcaacag tgcgcatata gttaagggca cactgcatct gtgaagtaag cgtgctcgca 44280 gcatcaagat actttttgac attttctgga gtagctactg gagtaaagaa ctcatgctta 44340 actgtaataa ttacctggtt ttccccagac tctttaacct tgaccatgtg ctcagccaaa 44400 tgggagatag tgcgataatc accatccatc aattttgtcg ctgattttat gatagacatt 44460 ttgttatctc tgaattcacg cgcccagcga tgggcgaatt cagaaacagt atagccttgt 44520 ttatcaaggt agtatttttc aacgtcacca aaccaatgat agccaattgt acctttgccg 44580 cggttggatt caacaccaaa cttatgaaga aggtctttat taaccaggta tgacaggact 44640 cgggaacctt taaaagtttt cttgatatca tcgttacctg cacgactcgc aatagttgca 44700 atgtctttct tgaagcgacc agatgcaaca atcgtcgagt caatctgatt taaccagaaa 44760 ttgataatcg aaaggataga caattcacct gttaatggtt ttacttttgc gtcgatagtg 44820 gtgttaaagg cctggatata ttcgttacgt gtcatagtgt tctcctcata aagtaggaat 44880 tcattatact ccctccatcc ttggaagtaa acattatttt aatgctaaaa tcgccgcctg 44940 caatttattg cgttgtgttt ccatgttctt gatgcgattc agaacatcct ggataccctt 45000 tttctcatct tcaatcagac catttaagcg gttgactgat tcctggatag catcagcatg 45060 atgtttctca ataggcttca ttgatgggtt aaagattcca agagagctag ccggtttgac 45120 tttccattgg tacatatttt taacgtccag cggtgaaata ggctgtgttt tatattgacc 45180 tgcaaatgct ggaccatatg atgcggatga tgcgataggg gtacgttctg tcatttctgg 45240 ttcctttttc tcctgataag gagcgtaaga caaaagatat tctttagtca atttaatatc 45300 aatggttata gttttaagga attttccgtt tggtggatat aatttgtatc ttcttcaaac 45360 gtgtatgctt caacagcatg gcgcatataa aacagcgcaa tacccataac ctctttaaag 45420 gtatcacaaa gagcaacatc acccttggtg gttggcttga acttaatctg cacaccatca 45480 ttcttgatat tgataatcaa gttactggag gtaaaattat agttcttgat gttaactgga 45540 gctttacgaa tacgaagtcc agctgccagg atttccttga tactcttacg aactgtgaca 45600 ccaaacaaaa acgcagggcg gcggcagtaa tcattcagag agttaattct tacaatacgt 45660 tgaccatacc cctgggtggc agaaccagat ttgattttgc caaagttaac cagagaattc 45720 atcgcaataa gacgggttgc acgtccttca cgctgttcaa atcgagaata ttctttacgt 45780 gttttaatgt tcatatcaga gtccaaataa tttttcatag agtttagcga taggccacag 45840 aattagcata cataaccagg caaagcaaaa tactggccat acaaatgtaa tgaataccgc 45900 atcatagctt gattcaattt taaccgggtt ccatgcgcct gttttatgcg caatcaaagc 45960 agtaataaat cctatcacaa gataagcaag aataaaacca ataatttcca taattaatcc 46020 ttaagaagat ttaggatatc gatatatttc tgacgttcag ccttggcttt agcattcaga 46080 ccggagagtc ggaaaatctc atcatcttgt tgttgaattg taacattcaa gcaagtaagc 46140 aaattgctaa aatactcaat ctggacttga tgcttctcat tgcttcgcac tggaacaggt 46200 tccattttag gagcttgttc tgaagagtct tcagccaggt ctttaatttt ataaagttta 46260 atacgctgac ctgctccagt ttcggcatca ccaaaactaa gaacaccgtg ctgttctaat 46320 tcagacaagg ccaatcggag atggaacttc cagttgtcgt attgacatcc actgtaatgg 46380 gaaatggtat actcattttc atttctgggg tggttaacaa tactaaagat aatccaatca 46440 cccgaaccat tggtcttaaa ggtcatttgt ttatcttgac cttttgcaag gcgagcacca 46500 agagccagta aacgtcgttg gttggcatga aggtctttaa ccacttcatc aaacgtcatg 46560 gtgctaatcg cacgaccata aaggtcgtaa cctttgttat aatttttaag aataattctg 46620 gcattagcac gagacaacat acctgtttta cgaagagttt tcatgactcg gtcttctaac 46680 gtaaacgaac ctttagtctt gtcaaagatt ttagtgaagt catttttaac agtacaaaca 46740 ttgaacttgt cccacatcat atatagaacc ttttcaacca catctttagt gcttggaata 46800 ctgttaaatt taacaacttt tttaacttca gaacgaattt cattaattgc ttgtgacata 46860 ataatttcct caatagatta atcaaaggtc agaaccatta taccatcctt ggtataaagc 46920 gtttatgcga gaaccgtctt taaacgctct tcaaacttct gaaggagtac ctggcgctct 46980 gctctttgtt tggtataggt ttcaatacgc tctgaagtga gattgtattg ttcattaact 47040 gattccctca tgaattcagg aatctcttta cacgccttga tttcattgaa cttatcaagc 47100 accgcttgtt gttcagcaat cagaccatca taatattgga tatctgattt gatgttttgt 47160 acatcagatt cagtaggcat atttttcttt gctcttgtcg cttgatattc tgcattagtc 47220 tctcttgacc agaacaaaga acggtttttg ttggtgtttt tatacacatc aacaatacca 47280 actgaatcaa cagtcacaat ccagttccag gcgtttttat aagtctgacc acctgatact 47340 gtaatctcac ctgcacaagc aacatcatta aagaacttac tttgtgcttc tgatttaaaa 47400 ttaccattat tgtaattaat cagattaaag attgatttgg cttccatttt attctccagt 47460 atttggtaac tcagtatggg aacattatac gtttatcccc atactatgta cacatttaat 47520 taaaagttct ttcagtattt tcaccgctga accagacgct cttaacggct tgctgtgcgc 47580 gatggagctg ctctgccata taaacaccaa gggcctcagc tttacgcgtc ctgtgggctt 47640 ctgcggcgac catttgcagc ttatccatca tgataagagc tttacatttc tccttcagag 47700 catatcgctt cttctcttct ggagttttga ccattcctct taacttgtgc attttcagct 47760 taaaatggtc agcagatttc agacatgcct caatcatgtc aagttcatac tttttcataa 47820 ttcctcactt gcgacgacgt tttggtaatg gcattgcttt acctgcaggg gtcaaagtca 47880 cacgcaaagc attaccaata ccaagggatg ctttaaccag acctttggct tttaactcac 47940 caaatgcttg acgttgttca gggtcacgag tgttaaccat cagacctttt tcaccagcag 48000 ctttgattaa ttctaacatt ttattctccg tttgtttatt tggtaactca gtatgggaac 48060 attatacgtc tatccccata ctatgtacac tactttatat aatttttaat atcaatcctg 48120 ccctggtagt gcttggcagt ttccttcatc ttttctatgt cgataccaac aatcacctca 48180 catgattgac ttccatgatg gcgtttaaaa tgttcaatcg cttcttctcg cgtttgtttg 48240 gatacgcgag gtctcatatg attcataatg gctccttagt aaaaattcat tatgcatcaa 48300 cagagacaag tttgtacacc tttaaaataa aaaaggaccc cgaggggtcc ttagattgca 48360 ctccaaaaat tatggaatct tatagtattg atagcccagt taacaaacct gcgagtttgc 48420 ttggaagttg gtttacatgt gtacttgata ttgacccgac gcccagtttc ccgacagacc 48480 gcttcgagat accagcgccg acctttattg ggtcttccat atgataagaa gaccttgatt 48540 tcctggttat cgataatagg aattgtggtt gtcaagatat acctcttcaa caaacttgtc 48600 ccagaacttc atgtccacct tctcaggcat accattctta gcggcccaga tagcattagt 48660 ttcgacttca tcaacgatag cttcaagttc ggcctgcaca tctttaaaag catgaagccc 48720 ttgtttgatt tcaaggataa atggtgctgt acgtaaagga tattggaggt cgccagtcat 48780 atagatttcc ttcaactggt atccagcccg ataagcatgg ctcagagctt tccagtcgat 48840 accttcatta gcttcagcct tacgagcgcg ctcgccatat tccgcatcga gcttattcag 48900 agattgcttg agctcgatta aagacaaggt ggtttggtac ttacgtccaa gaacagtata 48960 gaacgtttga ggaccagttt tctcgtggtt atggaatacc cattcacaaa actcgttttc 49020 agggagtctg tgtttgatat cttctacctt agttcgacgt tgcttagtcg aaccgtcttc 49080 ttgataatcg acccactgtt caggaatctg gttaacgatt gccagtacac cacgtagagc 49140 ggccagtcga gaacctttaa caccatattt ggaagcttgc ttacggacat accccaaata 49200 ggctttcatg ttggttgtat agaaccgcga acggttatcc tgaataaact tccatacatc 49260 tggtaaatcg gatttgacaa ccaggtcagc cggtgtgtga atcatgtcca gggcaacggt 49320 ttcgccatcg gctgccagtt taaagaaata tttcagacta taaagttcat ggtcaacatc 49380 atcttttgta tttttggtag ccgtgttatt cgtgttcagg tttgtatggt tcatcgcggt 49440 tccgagcagg atatctcgcg gatgaggaac gaagatttct ttaaaatcaa catcagattc 49500 cggagtactt gttccataaa ggtgggagcc aaaatatccc ttcataacag ttctcatttt 49560 ttgtcctcga agtggattac agggcacatc ttagatttac gagctttaat gtattgaata 49620 acgatgtttt ctttagtttc ctttccttca cgtttggcta gaatacgttg ccattccttt 49680 tcttctttac gtaaatcata ccaggaccat aaattcatta gaccatacat taaaccaaat 49740 gctaccgcca gaataagagc cagtgtagct agtcctaaaa taaaccctaa acctaaacca 49800 accggggtac ttaatacaaa gaaatgctct aatacaccag cccccgcgac agtgaatcca 49860 aaaaccacac taccgataaa aatggcacaa acaaaacagg cccaaaccat tttccaaaag 49920 taagcacaca atgagtgtgg acgagaatgg cggtcataaa atttatcgtg gaaacgagca 49980 tgccaagagt ttgcattgat aatcataata ttttccttaa gagttcatag ttgggataac 50040 cgagttcaga taagagacta aaacttttag ctcgtcttta gtaaatacaa cttcactttc 50100 accgtcatgt ctttggacaa tggacaacaa gtcacggccg ttatgttgat acacagtcag 50160 ttcagtatta taatctaatt cctcttcatc gccttcaaac gtttcgctag atcgaatcag 50220 tgcgtcttga ccagggactt tccattcaac ttctggcttg cattcttcct ggaacagttt 50280 gaaaatacaa tcaaagaatt tatcagaatc gctgatgctc agacattttt catctgtttc 50340 aatattaaac ccagcgtcgt gcttataaat gcgagcacat acatcgtcgg taatatcaaa 50400 tacaccacta aaactaccgt taagatattc atctttatcc aaaaccatta aatcataaag 50460 tggtttaata tcagcgatag tcagtacgat tttatcgtct tcttgacaaa gctcaattgc 50520 gttattaact acttcagcgg tcagttcgcc aaacaattca gttgtttcaa taatcatttt 50580 ctgttcctca acggttatgt tttaaaatac tataaagcgc ttctacttgg cttgcatcca 50640 ggtaaataac atcatcatct tgctcaatgg aaatcccgcc agtattacct ttaacaaaat 50700 tacattcgca cacttcaact gggcggtata cgtcaccaac tggatgctca ggctccagaa 50760 caatcacacc attttcagaa ttaccacttg ggtcatataa cattacagtt tcctcgctaa 50820 ccatgaaaag aataaaccaa ttaaaacagg ccctacagcc attgttaacc aagcccaagg 50880 ttcaagatag taaatcatct catgctcctg tgccagcaat ttcgtggctc acgtttaaaa 50940 attttgtcgt gcattgattg acaaccaaca caatatatca cacctttaat cgcttgtctc 51000 cgcgcttcag gaattttttc gtcgcagtca aaacagaatt cagcagactc cctttcaact 51060 gcaacttgcg ctctcgcatg ggtgatagcg gcttggacaa ccgcttccat ctctaactgt 51120 tcagcaccat cttttgcaaa tccaacagcc attattcttt caactcattc agatactcaa 51180 ccaaggctgc tttagagtta gccaaatctc gtttacactg ttctattcca gaatatgaat 51240 aaccttcaca atattcaaca gccaggtcat tggatgcatt ttcaatgtgc caggcgagtt 51300 tgataatttt ttcaatctgt tcagtagtta acataatatc accagtattt tttgatggta 51360 gaaattaatt cttggacttg agtgttcttc agcttgcacc cacaaccttt taaggtgtct 51420 ttaaggagcc atggttcctt gagctcgtcc ttttccaggc ggattctata tcctctgtaa 51480 tcagcaaata tgcttccgtc atcaaatatc ttaatttcaa gaatatgatt attggtttca 51540 tattccaatg aaaccatggt atttgtaagt ttcattattc caccacatat gataatgaga 51600 aaattgctga tgaaagatgg gtagcatctt cttcacacag ttcaaatgga gctttctgta 51660 agagttcaac cacttcttca tcagtggcac tgtccataaa gattgcataa tcattaagat 51720 agataacaac ctcatcacca gtattaactg catcaatctt gatattttta attctcattg 51780 tgttctcctc agtagttgat gggataatag tacacccttt ttatggtgtt gtaaaccccc 51840 taaatgaaaa aaggtccacc cgaaggcaga ccttttgaat tactctaatg ttaacagata 51900 cttagtctga aagaaaactc caacgatatc gtccagggtc gattgaatag ccttgggtac 51960 aactttatag actctttccg agcttttaat catttcatct aaaaacaaaa ttgtatcttt 52020 cggcaagtcc gaggcggacg gtaatgcagc tttgtattgt ttcccgctgt aacctaacca 52080 ttgctcactg aactggtcga gtggaccttg gatttctgtg aagtagaaat cataagcttt 52140 atggcgggcg taacttttgg tttcaagatg ggcggactta aaataagcca aagagactaa 52200 caaccaccca atatatgaat ccacctcaga cccacgaccc gttacgaaat catcaaattt 52260 catcttccaa ttttaccttc attaatttag cgtactctct cgccgcagct ttaccatcgc 52320 ttttgcaggt aatttcagtt tcgcattggc ttttgttgtt ccattcagtc ttgataaacg 52380 cccagaactg ttcggattgg tgtacgcttg gttctttgaa ttgcgaataa atcttcatcg 52440 cactatctgc atattgggta aacttcccat ctgttgcaga ggacagattt ggacctaaac 52500 agcccaggat tattagactt aatgaaagtc ctcgaataag gtccataagg actcctattg 52560 ttggtcatga ttacgctttg cagccttaac atagcggaat actagttagt aactgtattt 52620 atagacgttt aaaattaaag cagtactctg aaaataaacc tgcaaaatta cgttcaaccc 52680 agacccgttg ttttggactg gtgaccgttg acaaatagta tgcttgttgc aggcttattc 52740 cgtttttatt ggggcactct ttgttgaacg agtgataggt acttgcaacg atttcgactt 52800 cgcgctcata cctttcctgg tcctgttgca taggaacaaa aaccaaaacc aggaatatca 52860 aaatagagat atacgtccaa accttgtata ccatgtgaac ctcagcttgt taattcgagc 52920 caatgttttc tgcacaaaga tacgtacgat tcattcccac cgaccaatac ttgctcgccg 52980 tcctttacag cagcaccgtc gattactcga gcggtcatgg ttgctttccg gccgcaatgg 53040 cagacgccct ttaattcaac cagtttatcc gaaatagcca gaagtctggc cgaaccggga 53100 aataattgac cccgaaaatc tgttcttaac ccgtaacaca taacggggat attgtaatta 53160 tcaaccactt gagctaactg gtcaatttgc tctggtaaca aaaattgagc ttcatcgacc 53220 aggacacaat ggatatcctt ttgagactcg gcccatttat agaattcaaa caggttcatg 53280 tcggccgtta ccaggtttgc ttcctggcta ataccgatgc gtgatttaat ttcgccttcc 53340 gagtcccgat tatcaataac aggttttagc aaaagcacac tcattccacg ctctttatag 53400 gaataagctg cagtcagcaa tgaagctgtt ttgcctgcat tcattgctgc gtaatgataa 53460 attaaactcg ccatatcacc ttcttaaagc gtattcacat aagctattaa ttcgttttct 53520 ttcgcatcaa atcgattagc aaattcttcc tgatgttcgg catctattgg accgtattca 53580 tcataaaagg cgtttgcttc tgtatgctca tccaaaagct catggataag ctcaaataat 53640 ctacttttct gactaatgct taaactcatt tcggttcccc atatcgcaca aatttaggat 53700 gttcgcaata accagaatga aaaccttcac ccttatgatt ggaacaccct ttattggaac 53760 agtaccacca gtaatcatat tcgcaattca tttcatcgtt gcataaagct gcgattactg 53820 gccattcaca tttttcacat ttggccgttt taagaattcg ggcttggctc ataaccattg 53880 cacctttaag caatattctt ctacgtgctg tttacgttta tttttatcta taaaagtata 53940 ttcaacatat tcaccgatat accatttatc agtgttgtgt tgacttttta taggacattt 54000 ggttactcga gtttcggtcc agcgtcgaat aatttccgct ttgtttttag ggtcaaacgg 54060 atgtgggtaa tgtgctttca taatccacca ctattaaatc aatgtcagga gtaaacatat 54120 caattaattg agatacctta tcccaatcac caccagcaat accacaacca atacgtggaa 54180 tataaattac cggtttaaac aaaagggttt ctgcttgtag atttaattct atcatacaat 54240 ttactaaagc gccataatca agattagggc ctggttcgta ttgcgtatac aagttatagc 54300 acatgccaaa acctgtaggc ttatgtttaa aaacaataga catatcgcct aatttatcac 54360 gagaaccgta ttcagtagtg gttttatcta tttctaaaac ttttggataa gccttcgcca 54420 actgtccagc tacaccggaa cccattgtat gaaagcagtt gcaaccatgt gcaataatat 54480 tgccttgaag aaagagggcg acgatatcgc ccttaatata ctgggtaatc atttagagtt 54540 caatcctcta ttatatgaat ccactaacct gtcaaccata ctggtgcagg ccgccttgta 54600 ttttgcactt gatagaatgc aatccatttc aaataatttc ttttcatatt tgttggataa 54660 tacattcttt ttcaaaagcg tatgttcaac atcattttga ccatctttat aggctgcggt 54720 gagcttggca ctgaactgga ttatacaatc cacattgctt gctgcgcaat aatctttagc 54780 cgtcctcgtt gcatattcta tgatgctgtt gttgtcgtcg ctctgtactg aagctattcc 54840 tcccatgctg aatactaacc ctatgactaa agtgataact cttaccattt ttaatccacc 54900 gtttttaata ggcctcgtga ttttaagtag ttagcttttt ctaggacctg tgaagcatac 54960 ttattaccag ctttcacatt ccatcctgcg ttatatgatg atagcgtttt tcttatatcc 55020 cccttatgaa tattcaacca gtatgaaagc tcaatataag cccagcgtgc actgttctct 55080 ctcttgctta tcatcttgat tatttcttta tctgtcattg tccacccaac ctgtttcact 55140 cgttcgcgta cggttggaag ataattttgg aatataccat atgcatgatg cttatcttta 55200 tttaatccag tattaacccc agcagaactt tcctgccagg ttaaaccagc catgatatat 55260 cccaaaccat tacgttggtg tggattttct ttgtacttgc catttttctg gaactgttct 55320 ccaaacgcat aagcatattg aaggttctcc atttgttcat tactgaaagt aggctctacg 55380 ctatgggcag acatactaac tgtcaatagt aaggtggcga ttactttttt catgaattcc 55440 ccattagata taaatcactt ttgtgctaat ttttccttcc agacgattag agttaacaaa 55500 cgccatacga caacacatgg ttggagactt cccaggaata gctggattaa ctgtcattcc 55560 taaccatagg ttcttgtctg tgatttccag acgaagtgga cggtagttca cattcgggtc 55620 aatttgtgct tcaacttccg ggagtggttt catctccagg aattcaacga tttcttttac 55680 atgtggtgtg atttttccaa acaagtcaaa aacatattgt tcatcaattt cacgttggat 55740 tgcacggtcc aataggtgtg gactgtactt aatgaagaat acttcaaggc ctgcttcttt 55800 acaagccttg ttgatagcat tattaatttt gaggaactcc gtttcaaaaa tgcgacgaag 55860 tttattgcgg cgaatgtaaa cacttgaatc gatagtagtc atgattatct ccttttgttt 55920 gatgggataa tcataactca tttcaatctg cttgtacact actctttaaa agaaattagc 55980 ttattcattg cttgggcaac ttcattacga acagcgtgta aattcttaat ctgttcaaga 56040 cgttgctcat aatactcgat ttcatcttct tccagacaat cgtctttatc ttctttaaga 56100 tatcgagcat agtcctggaa agcttgacga atcacttctt gaaagtcatc taacccctgg 56160 attttcttgg gttttgatgt gaatccaaac ggattggaat cgcttatgcg gcgatgagaa 56220 gctgttgata actcatagcc aaaaccagaa ttaatctgcg ccattagtat ttttcctcag 56280 gttggaaaac ggaacgacac gcccacatgc ttgcttcttt cagacggtca attgcattac 56340 gaatctgggt gatgcgttca tgagcctggt ccagttcttg ttcagaaacc tcttcggtat 56400 ctaagttcat ataatgagta acatgttctt cttccagagc tttaaacatt aacccaagac 56460 gaacttcagc atctttaata gcattcactt taccgatttt atcgtcggta tgtggtttat 56520 aaccctttat atcttcaatc atttcacttc ctcgccagtt gcgatatcat attctaccaa 56580 acgaaggcct tcagtccaac cttgcacgga gatataggta gacgggtaaa tcatttcttt 56640 accattccac cgaccgccat cccagacttc cagaaagtct tgttcacctg gtccattaaa 56700 ccattcaaca aatcttttca gagcttcatc agagccttca attgtcaatt tagacatatt 56760 ttttccagtc actaattaat cgcacatcct ggtcataggt caggaggcta cgagtgataa 56820 tacccttaca cggtccagaa ataaattcca gagtaaagta aggaacctgt ttcattaatc 56880 ggatacttgg agcatgacat ctaaaacgag agcccttaaa gggccctgac atgaatacat 56940 attctaatgg atagaaataa gcatattcaa aatttgtagt aaggtcctct cttcggacct 57000 tacgtgagaa ataacagaac tcaaaaagtt cttcatcagt catcattgca ttcctatcac 57060 aaagaacagt ggcagaggtt tagaaccttg gcacataaat ttattcatca ttgtctgata 57120 agcttcatag aacttatcca tcgctggtag gcgaataggc tcttcaataa taatcaataa 57180 tcgggtaaca gacagtccac gatatttgtg aaaatgatct tcatctaaaa atgaacgaac 57240 agtatcatat acaacaaaac caggtcgttc aacattaaga tgtggctgtg ctttagaaac 57300 ccgctttatt atatcacaga tttctcgtga gcgatgcgaa gaactcgaca taacaatcac 57360 atcattacct ttggaaaccc aatccgcagc aaactgtgca atggcacttg ttttacctgt 57420 ttgtcgccca ccgtcaagtc tcagagtgcc aaactcttgc attcttgaag cgggttcata 57480 ccaggtgtat tggtctgaat ctaaacgttc aatggcgcgt tcagcgaagg cgtgcattaa 57540 ggttagataa atgggagtta aaaatttcat aattgtctct tagttggtgg ggccgttcct 57600 tggcacataa ttcatccatg tatccgtatg taaacccttt accgcacttg ggctcgacct 57660 tattacaggt agggaaaggt ccatcactca gaggacgcga cgttcatgag gagagggccg 57720 gcttgccttt attagaaaga accagagcct ttaattgtgt tctggcgacc tttattttct 57780 aattcagaat ggtcaatttc atatgcttgt gctacaccaa aagccttttt aactttagct 57840 aaagaacctt tagatgtaac aatattaaaa gtattacctt tggtaaaatc aaccaaatcc 57900 acttgtacac cttgtttacc gaggcttgcg acaatagcat caacatattg ttggtctaaa 57960 ttgccctgga ctccaatttg gaatgcttta ggagctttag cttctgtgat aaattcttga 58020 taagttttca tttattctct ccaagttgat gggataatta tgcactatcc catcaaaaag 58080 taaacagtta tgctttaccg ccagaaacaa taccaaaggt ctcaaagtcc cattcaatat 58140 cgtcaacatc aactttctta ccagcaacca cgaatgcgat ttcgcgacgt tttgcatagg 58200 tgacaatttc tttaccgtca ccaccaaaga aagaataacc attgcgcttc atggtgccaa 58260 agtcttcagc atcaccagca aacgcgcgga ctttaaagga accggcagga gctttagtag 58320 attcagtaat aaattcttga taagttttca taatgtctct ttaattactc tttagggaaa 58380 tcgcgcaaaa acgcatcata aagtttatta ccaatgcgac ggaatgcttt tgccgcatga 58440 acgttatccg gatttgcaat tttaatttgc attacatgac taccggccca tgatttggct 58500 tcatcataat agcctttaat ttctttaata gtcttacgtt ttccgccagg accatcaaca 58560 gtaattttcc acgaaccggt gcttgtttca cttggtgggg ttaattgttc aatccagaca 58620 taaccgcctt caacacgttt ttgggtagat tcagtaataa attcttgata agttttcatt 58680 ttatattcca ttgtttggtt gataaaatca ttatacaacc tttttagtta agcgtacaca 58740 gctttcccat agtatttgta ccaggtaggc ttttgagaaa ttttctcatt cagtctggct 58800 tgactgattt taattgacca gattgatggt tgataatcat tcttccattc atcaggaata 58860 tcatttaaat cttgggtaga aatatctttt atgttaaacc ctcgctttaa acattccgca 58920 ataagttctt gatgacggtt tttaaggaac aaaagtttat cataaaagaa ggtgacatga 58980 ccagtcccta gaagaaaaga tgatgggatt ttaaagtcag aaaccttttt accattttgt 59040 acatgcttac gaacagcacc aaaaacgcga ggaagctcac gatattcagc cataagatgt 59100 tggtcagcaa gttcagatac cagagttaaa ttaatgcgag tcatgtttgt tctccgtagt 59160 tgatagggtc attataaccc taccatttgg agatgtacac taatatgcgt caaaagttcc 59220 agtacggaaa gtttcgataa ctcgtttggc tcggttcgga gtctggactt tataccatct 59280 cgagtcggcc agttccttag cggcggcttc ccatgattta gccttcagca atcgcatacc 59340 cttaggaaaa ccagcaacac cagcaacacc catctggaat accatattaa tgagcgcaca 59400 ccgacggact tcatccagta catcataaac aggttttaag acaggatgcc tcaagattcc 59460 agctttagcc ttttcgaccg aacggttaaa cagtgtttca gcttcgtgct gggttatacg 59520 gccattacag acacgaccca tgagcttgtc gagctcggca cgagcaacat cttttgatgg 59580 gttcttagtt atcagttgcc cgataccaat ggtccaaaag ccttcggtat ctttatacaa 59640 atccaactta caaccttcat ctatgcgtaa catttcgaat atattcataa taccccctta 59700 tataggggta tttattatcc gaaaatttga gtcagtacct tatacatcga tttgcccatc 59760 acgtactgcc attggctcgg tttaatcaga gcaaacgcat caaactctgg gaccgaactg 59820 ccatccggga aggtatgata ggcagagcat ttactatgtc tgaactgctc atgctcgacc 59880 ggaaccgtgt acaaaaacag gtgaaggttt ttattatctg aataacggaa ctcaccgagg 59940 tcttttaaga atgctgggtc atattgggta aacccggttt cttcttcggt ttcacggata 60000 gcagcttcaa tcggggattc atcagcttca acatgaccct tagggatgtc ccatttgtgc 60060 gcaggcatac ctggtttacg agaaccggtc acacgaccca tgaataactc tttatcttca 60120 gtcatgaaaa taatgccagc agataattct ttcaccttgt ttttcttact catcgtctcg 60180 tttccatgat tggttaaaaa tatcgctcat taaaaattca agtctatttt ccaatgaagg 60240 gctttcacta tcagaaactt gaatcatata atcagaaata ggtcggaaca tatccaagtc 60300 gctttctaaa tcttctttga ttttctcttc tgatgttccg gtgtcatagg aatacagata 60360 agaatcacac gactcagatt taatataaat tgcaatactc atttacggca ttcctcaata 60420 aaagaattta atgtattttt cttagcgata tcgtcgtata cctttttaac tttagaaata 60480 cgaaaaggta cttcaatagt catagtccat aaaaatgatg caataagtcc aacagcagca 60540 gaagaaatgc acgcgcccat tacagccaca ggcgcttcat ttttacctgt caggaaaaac 60600 cacaggattg gtaaaaataa gattccagac caagcataaa acctggaaca aattaactta 60660 tcaaattcat ctttccaggt acgaaatcca tatttcttgc tataagaaat taatgggtcg 60720 ggcgtctgtt ctttgagtgg ttctggttga tacccattaa tgatgtatcc cattttatat 60780 tccttaatta aaagctcttt cacatgacga ttgcatcgcg accaatcaac attttccacc 60840 ttgattcctt catcaccatc atcaaggttc gcccaatatt ttttggtaat cataaattca 60900 tctcttgata caattatacc tgcattttct gcccgacgga tgaagtttac atcaacaccc 60960 atacaaccag gttcatacag cattaccagc cctccatagg aaattccaga tatacttgag 61020 cattaacgcg gatttcattg gtgattttat taacgcggtc agttttacga gatacacgac 61080 caaaccagcg ccaaccatta ccaactgttt ttgttccagt atgccaggtc tgccagttga 61140 attcacacaa cgtgcggtca ggagcttctt gaactaaaag ttcacccgcc ttgatttttt 61200 ccagaacttc ctggtgccac tggcgataga taagttcgcc atcaggaatc tgactgaatt 61260 ctgaagttcc cagagcaaaa tgagtagggc aaacagaagc gttaaccaga cccaggatgt 61320 gttcagaatg gtaacgaggg ttatcataat caggttgtcc tgctgtaata aaatgttgtc 61380 ctgctggaat atctggacgt ggtacatcat catggtgata cccagggata gcaggatacc 61440 aaccaggcat caacatatgg acgcgagagt caaatacaac atcacgattg gtccagtcca 61500 gtggtaatgc agcaataaag ctacgggtaa ttaaaccgcc tttatcccag gcaaaactta 61560 aatcacaatt aaagaacatc ggttcatttt tgatttcgtc attactgaca tattgagcaa 61620 attgaccaac ttcacgcatt tgactattaa aagtttttga gctattcatg atattttcct 61680 cagtattctt catcatattc aaagtcttca tcaaactcgg gttccagaga aatttcttct 61740 tctgggtgga attcacaagg gaaatattcc acttcaccac aatcggcgtc ataggactgg 61800 aaatcaattt ggattacggt ttcggataca acaataactt cagcaaccaa gtcacctgca 61860 atttccattc caactttaac atctttagct ttgatagtat tcattttatt ctccaatttg 61920 gtaactcagt atggaatcat tatgcactga ctccatactg atgtacacac ttattttgtg 61980 aaaggacgaa ttaaatgttg gttagcctta aagcgtttaa catagtcttc gcgccccagg 62040 gtgttgtaca tattaaccac ttcctggtaa tgttcccagg catactcacg gaaccatcca 62100 gacgtaatgg aggtgcagac tttttccagc gcaatatgga agctcacaga tggagagata 62160 ttgaactcat taggaacctg cgaacgctcc agagcgagca cacatgcttc ttcataaacc 62220 cctgccagtt taataacatc agggagcgct tcaaattttt cacgcgaggt catgacttct 62280 gaatcatcct tcatatagaa cttatatgca ggcttatcag caagcgccac ggcctcgtgg 62340 atggagtcat ggtcaagggt atagattgta tcattaaaga aagttttctt gcccacgtct 62400 agtttagggt gactgtatga cagtgtttct gtctgacgat gcagcatgat tttctgcagt 62460 tcatcattaa gaacaatacc cttattgcga aggtaattaa tatgactcat ggtcttcagg 62520 aagaatggat tgttcttcag gtaacgatga gacaatttga ttgccaaaca aatatcaggg 62580 gtcgcccagt aaaaaccaga tagacggtct ctctgaatat ttgccttcgc atatttgagc 62640 agacgataac tggaggtaaa atcttcgtct tgaagctcat cagtcaactc acgaacactt 62700 gtcagtgcgc ggacgatgta cgcttcaaaa taggtttctt taccattgtg catacattta 62760 aaagcttgaa catctgggtt atccacatgc acgtgctgcc ccatcatctg ggttttaaaa 62820 ctgttccaag aaccctggtc agcgataaag tcccaatcag agttttcaat tttctgactt 62880 tcaatcagac cagcgttatg taatgcacga gaaccaataa ctaacatcat aatattttcc 62940 tcattagtaa agacgagtta cttcatattt tccaagtgcc gattttttcg cttcaattcg 63000 tgaggcagca agattggcgg cctctagtga agaaaattga accacttgag aagtattctg 63060 tcttactccg ccttgcgggt gagtgacaaa aacaataaga acattataca taatattttc 63120 ctcaaataat accgtatgcg taaatatctt ttttaatgca ggaaactact tcttccagag 63180 aatcacaaac acgttctgtg acgcctgcgt aatcaccact gatggttact ttaatgccat 63240 cagaaacttc ctggagatta acctccagtt ggttgacttt ggcttcgttc agaactttga 63300 ttaattcgtt tgcgatagac atttttattc tcctgttctg ataacgtgat tgtcatcagt 63360 tgataggagt atagtaacac agttttttag acttgtatac tgcttctaca atattctaaa 63420 aataatttat cgttgtctac cttatcccaa cgacgacaag atggattcca gaccagagcg 63480 ttgtcaccac aaccattgag tttaaaatgg tcgtagtaca taactatatg cctggtttcg 63540 tcactggaga accccggtat aggttctccg tgggtgtgat acctatgatg atgcattgag 63600 aggtcgcatt tgtaaaacat ctcacgttta ccgcgaacga acaagaaagc ttgcccattc 63660 atatagaatt ctttatctgt tttcaaaaga tatttgaata ccgaatcttt atgcataatt 63720 cctcctacca catccttgtg attaaattat tttcctaacg aaatgcgagt aggtttttta 63780 ccgaaatatt cttcatagga accatctaaa cgacccgctt tagaccgaca atacgaatca 63840 tactttttgt aataagtcaa ctcggtattg taacgtggat gtctatcagc cataataata 63900 cttaacggcc aacctttggc gtaatcacga agaccattta ttttacgaat atatcttttc 63960 tttaatttta acatgaactt gggtaccaga ttttaccatc ggcactagca acgaaggtgc 64020 gaccagcttc ttccccataa caagagctgt tcaaccagtc ttcaagacgg acttcgccgt 64080 cataatcatc aagctcaaca ccaaaatctt gagcagaagc catatctttt actttcttat 64140 acagtgcagc cagttcaagg gcggcttttt ctaattcagc acgttcaaca gcagtagtca 64200 ttttatcacc ttagcaagac atagaggagt taaaccagaa acctttacca tctgggatac 64260 cgtaatcacc acgaatccag tcatactctt cgtattctgg acaacccgca ggaatatatc 64320 gttgaagact gtcagcatac acatgaaagt attcaccagt tttatcggcc agttgttcac 64380 cttcacagat tagtgcacgg atttggtctt caatttcttt aacagtttta gcagtcattt 64440 tatttcctta gcattcataa gaggaggaca cccaggagcc acccgtaatt gtaatacagc 64500 cattgtaaat cgtgatatga cggtccagat ggttttcacc ttcgatatag atgtcggctt 64560 cttcacgttc ttctttacaa atctggataa cgttttcaat agctgccgcg gcagtacgga 64620 ttgcacaatc taattcacga gacataataa ttccttaaca ttcagaagag gaggacaccc 64680 aaccaccttt ttccaggtca gttgtatagt catgagtatt aacaatagcc cattcaggat 64740 gaccatgttc ttcccaatgg gcaaggtctg ctttcagggc gccaggagaa taatagtcac 64800 cgcccatacc gcgagcaggg tatatattaa aatgagtctc atatttgtct gcaatttcct 64860 cggcacgacg aatagcttta tggatgtcat ttactgcgtt agccagttct ttatttttac 64920 ggtaatcatt catcataacg tttccttagc acattgccga agaggagacc caagcaccaa 64980 ctgtaacgac accgttttca acttcaccgc cgttgttttc aatttcattt ttaaaccact 65040 gggagcattc ataaccgaca ggataatatg tacggcccga accataatca ccggtaccaa 65100 attggatacc atattcatct gcgatttctt cacagtgttt ttcaatttca gaggtcttat 65160 ttagaagaga atagatagct ttttctgcag atttctcatc ataatacttg gggatagcaa 65220 tttcaatttt attcatgatt tacattcctt aataaaggtt tcaagttcat tgcgttttgt 65280 tttgtctagg aacttattat actcagccat tgctgaagca tatctcttcc gactaggagc 65340 attcacggca tttgtgtgac agatatcata cacaacaggg ataaaataag acacaacagc 65400 cggagcgatt atcataaaga agcccatctc aataggcatc actccctcga ttgcagccat 65460 aaccgtggtc attaccagac caaccaggcc gccaagcatg gcggtgacaa tccctaataa 65520 taaattggca ccaatatcat attcagccaa ttgataaaca tgtgatgcaa gactaggccg 65580 ttccattacg aacctcctta ataaattctt cggcgcattt aattttgtgc tcttcttgta 65640 gggaaacaca tttttgttcc cactctttaa ctcgtccacg atatccttta ttcatatgat 65700 tttgatgcca cacatttatc aatatcatac acaggaccgt aataaaagga gctgcaatta 65760 ctagcgtcca ggcgactcca acactaatga tattcatatc cccacaaagc accatcatta 65820 taaccccaac gatgcacatg gacagagccc caaatggagg cataaggtct ttatcaatct 65880 tgtccgcgta cacaaatttt tctggtttac ttggcagata taacgttcgc attttgaacc 65940 cctttagcat attcacgaca ttcagcaata aaacgattaa gcttacgttt ccgtttacga 66000 ctctctaatg tgtactcagc cgccaaatca agaagctcat ctttatcata atttttaata 66060 tcagtataag ttggacgacg gtcaaccatt ccaaagtggt caccattttc taacatcaaa 66120 taacatacca aatggtcatc atcatccgca ttaataagtt gtttataatc gctagggtcg 66180 tttaaagcat atactgaata actcagccca gcgaattcca ctcgttgaat acccagattt 66240 acgtcaataa tttccagggc atctgtgaaa gaaaggtctt cagctaaagc aaaaataaaa 66300 ggcaattttg tagtatcacc gcggctagtt gttcttctta agaaatcatt ggaataaccc 66360 aagtcaccgc ggattctata aagaactgtg ttagtttcag tggcaagacc actaccgcca 66420 aaccagtcta catttgatag aaggtcagta tataagctca taatattctc ctctcccaat 66480 tagggggcaa agcccccttt tatcttactt aagcgaattg atatattctt caatgtcagc 66540 ggacgtggta tccacaccaa cctgtgggcc tttgaaggtt tcaacacgca tcagagtgtc 66600 ttcaatatca atcttagtca atgcagcgat ttcaacaaca tcatctgcgg ttgcaatacc 66660 cagtgcagac accttacggg tttcgcggat gtattccagt ttaacagcta gttcctgacg 66720 agagtcatcc agctctttaa ctttcaattt aatttcatta cgcatttcaa cataatcatc 66780 tgccttctta cgaagttgtt tagcagtgcg acgatacagg agacccagtt tagcatggac 66840 ctggacatct gcaccatcag caatcagttt tcggatttca cgctcttttg aagcggcgag 66900 ctggtctttt tcattcgcta actcgcggat gcgtttttct tcattaacag acttaacgtg 66960 ggcagtctgg agctcagtaa tcttatcaat cagggtagaa gcagctttag tatattggtc 67020 ttcaatagac aggttttgcg ccattgcagt acccagtttg gagcggatga attcaacaag 67080 tttctttaaa gtattcatgg tatttccttc agttagttaa tgttttatta tcccgggaac 67140 atactatcat gttcccgtta aagcactaat ttagattttg ttttcctgca caccataata 67200 aagttcatca gacacaatat ccaggatgcg gcgaacatat tcatcatggt attgacagtc 67260 aaaatcaaat gtaacagtag tttccgcttc acgagtgaat ttaacttgca cattcaggtg 67320 attaacaacg ttagttaatt tatcagtaaa gaactctttt gctaccgcct gttggacttg 67380 cactttgaac ttggtgttac gaatcatatt ttcagcagat gcgaaattac cagttttatc 67440 aaagtcaaca agcgccatgt tgatgatgaa tttcatttta ttctccagtg ttttattgtg 67500 taactcagta tggaatcatt atgccttaaa gcagggggct tgtatacccc ctttttgaat 67560 taaatttcaa attctttgcg gaagttggaa gcataagact gaactgatgc tttgttgaat 67620 ccaaactcac gcacaatcat atcaataaca gccctacgag cggcaccatt agacatattg 67680 acagttttga acatatcata gcataaatct ttgttgctca taccggagcg tttaggtgtc 67740 ttacgttcag tttttggttc agtcttagga gtaggtttaa cttctggttt aacctgtcca 67800 tcattcagac ctttaaaaat tttacctgcc agagttgcca gtttagcagc ttcgccacga 67860 gtgataccaa atactgccgg gttaaatgaa ataccttcag ctgtcagacg tttaaagaac 67920 atacgtgcgg cggattgtgc ttcagcttca gaagagttgt ttaatgccag tttaacatac 67980 ttaaccagtt ttgaagtttt cattttatta tcccgtttgt ttatttggta actcaatatg 68040 ggataattat acactatccc acctggaagt aaaacacttt tctcaattat ttttgagctt 68100 tgcggaagtt ggatgcatat gactgaacaa cagtcttttt gatatcatgg gtggtaacca 68160 gaacatcaat cagagcgcga cgagctgcat tatcattcat atcaatttct gaaaatgcct 68220 ggaagcaaat gtcacgagcg ttaccagatt tggactggat tttttcttct ttaacttctt 68280 caattgggaa ttcaatcatt tcagcttgga ttaattcaat gatagattct tttgactgtt 68340 tggtgtttac cttcagttca gaattaaggg acttggcgat ggcaacaatc tgggttgaag 68400 tcagatgaat aagtgcatct tcacgaatgc agatttcaca tacttcgtca gcaaccattt 68460 tgttggtgat gcgggtaaca gagaatgcag aggtcagggt caggaagttc attttatttc 68520 tccaaagttt tgtttgattc agtgtgtaac tcagtatgga atcattatgc acccggtcaa 68580 ttcggatgta cactactttt tgaagaaaat ttcaaattta ttcggggtat aaaaaaagcc 68640 ccaacctttc ggaaggggct ataccttgtc ggggttccac ctgcaaggca agtgtttgta 68700 cgaaacgccg ggattcgaac ccggttatta agcagttgac gctactcaat atttttaaaa 68760 ggccatatct cgaccatatc cgaacgttcc gtcaaaaacg ctactcggct tacggcaaag 68820 atatttcctc gaatcgataa ttcggtgcgc cgtttcagct gtgagataag ggactttaag 68880 aggtcaactc atgtacgcct taaagtcggg tcttattcag ggaacttcag tccgacgact 68940 taccggtagc gacccggttt ctcattcaat tgtaatagct aatttgactt taacaacctt 69000 ataattggaa tcaaggtcat tgaattccat aaaatttgtt gctaatctgc gagtcgggaa 69060 acttagatat ttattatgct caggtggagc agttaatgca aaccatggga cttgtttatc 69120 attttcatca aactcatcaa agctataaac cagctcgttg ctggttatat ccataactgc 69180 atatgattca ctaataacca taatatcctc agtaattggt cgagacagta gggatcgaac 69240 ctacgaccta ggacttagaa ggtccttgct cttccttctg agctatgcct cgtaatttgc 69300 ggacacacat ggattcgaac catggaccaa ttgattaaca gtcaactgct ctaccactga 69360 gctatgcatc caaattggtg gcccttgctg gtttcgatcc agctacctat cagttatgag 69420 ctgacagctc tcccattgag ctaaagggcc aattctatta tgaaaggctc tttgagggaa 69480 gaacctttgg taatagaggg tacttattaa taataacaca taactcttaa agcatattac 69540 ttgttaacct tcatcaacaa tagtatgtgt attggcattg acaatctgcc accagtcaaa 69600 acggttggaa ccataacaag gcttcttctc gttttcttta atgataacgc gcagttcatc 69660 ttcagtcgca gcctttgcga ttaagtcagc ataaccgcca cgaggataat agctatcacc 69720 cgcgaacaaa aggaaattta ccttcccaga agggatatag gcttcctttg gatacttgtt 69780 tcctgcatgg tctaccactt caatataacg ataaggaata tcagtatttt caatccattg 69840 ccacgccgcg gcaggagaat caaaagcatc tacacctaaa cggttatctt catctttaga 69900 cgggttattt tcataatccg catatacata atattcaacg ttcattattc acctttagag 69960 attttatcca taacgaaaga gattgaacca attaaaaatg caactactaa agagattata 70020 ttctcagaag ttgaaagcat attcaacata aagccagcaa acatgctaaa accaaaaagc 70080 ggtaacagca acaagggcag taacatttct aattaattcg caacgtttca ttttattctc 70140 ctcagtagtt gatgggataa tcatactaca gttatcccat cacgtacact aaatttttgt 70200 atattgtcca gctttaactt ttttcagttc acgagggctt accgcgtaag tgaatggagc 70260 attccaggaa gaactgactt tgtaaccttt agtcaacatc attgctgaaa ctttcttgtt 70320 atcaggaccg ttttcgcctt taacaagaaa caactcaaat gcgccaccat caaccggttt 70380 ggccttaacc atcagatatc ctttcaggtc ttccagcata tccgggtgac taattgattc 70440 gttaataaat tcttgataag ttttcattag tagctccagt agttgatggg ataattatac 70500 tgcaactatc ccatcacgta cattacattt ttacattttt gaaccacgaa ttacaccata 70560 agcggcgaag tcgtcagaaa tatctttagg agagttctta ccagtttcaa tgaaggcaat 70620 gcttgacttc tgactaaaag aggtaatttt gttatagttg gagtcaagga aggtataacc 70680 atttttacgc agctggtcaa agtcttcagc gtcgccttta cctttgaagt caacccattt 70740 gtaggtgttt ttcttctgaa gacccagagg ccaggtaatt gttttctacc aaaaattcat 70800 gaaaagtttg catttgaaat cctattttag ataaattgcg tattttttat aaccagccgt 70860 ctcatgacgg agacccttgt cattggcaaa acgctcaatg ttattaattt ggttctggtc 70920 aagttctgaa atgtcaaacg cgaaaaagtt gcgtttgtta ccaattgtta tatggtcagt 70980 caagtcaatg ctatacaagt gattgatatt gtcaatcata gctttgactg ctttactaat 71040 ttttacttct tcgttcagtt gggactcgtt gaaaaattca ttgaaagttt tcataagaaa 71100 ccttatttat aaagttttgc cagttcttta cgtccacgag cagacataat aaattgttca 71160 tcgccaattt taaaataagc cataccaccg tcaacagcga taccaatttc tttagtacct 71220 tctttgcctt tccacttgcc accagatttc aggacagctt caactgcagc tttggaatat 71280 ttggacgttt gaaaacgttc attaccaatc tgaaagaaaa caccatctgc atttttggtt 71340 aaaccaaatt tcagagagcc ttcactatca tcaattgatt tggtctcatt gatggcggat 71400 tcggctaaaa actcgttgta agttttgacg ctcattttaa ctccgtttgt tcgttggtgt 71460 aggaacatat tactgcacct acactcattt gtacactact ttaaatccgc cgaccaattt 71520 aattttagcg gttctttata ttcacggtcc aaaacaaccg gaatctgaac ttctccttta 71580 tacgtcaaag gaccaacatt ataagacaat gttatgtgag gagtgtagtc cgggaaatcg 71640 tgtgttgcgc caagggctcg tgcatacata tgtctgcaac taagataatc tgaatccagt 71700 acaagaacca gggtactacc atcatcggtt ttccatactt ccaggtgccc agagttggct 71760 acttcgaaac taccagaggc aacaacatat ggaatattta ctctggaata acaaatagtg 71820 gaatggaatt tttcgcgagg aacgggatta ggtaccttaa gagagcgctg gagattttcc 71880 agcgcatcaa gagttaattc tgaaaatttg gctgcaacat aaagtccttc agagacttca 71940 tttaatgtca ttattcttct tcgtcagcac tttcagcggt caaatcagga acagcggcaa 72000 tcaggtcttc aatctgaatc tggtcaccgg tgatgcctaa gcgagcagcg attttactca 72060 gcgcatcctg cagaatacgt gattcctgtt gagcttgagc tgcaacatcc tgggtgtcca 72120 gaatgcggga tttaagagct acgatttcgg cttgcagttt ttgttcaata gtttgttcag 72180 acattatatt accttattaa ttttatcaaa aagttcatcg agtgtgccat cgtttttaat 72240 aacaatgtca ctagctagtg gtggaagtcc agcttccgta atgtgtgtat cagtagaatg 72300 atttgcttca tcgcgttcta caaagattat tgtagcaccc atcgcccgaa gcgaatcaag 72360 ttcgttcttt tggcgaacat ctgggacaac gtaatattta tatccagaat agaggttgtc 72420 aagatagttg catgcaaaag ttttaatcca aaacatgctg tcgaactctg tacaaacgat 72480 gtcagttcca agggtctgca tgaaacgtct aactgtccag ggctcaacat tatttagtgt 72540 gtgaaattca ataattttac gaaccatatc tggattcgga aggtcaagat tgtattgttg 72600 tttcaaccat tcaagagcat caaccagaag aatataagct tcggtgttgt tcattatcaa 72660 ggttttttca cggtcataac cttgtccatc aaaattttcc agggtcaaat tgtgtggcag 72720 gtcttgacga atccacgcgt tataaagcgc ttctttaatc ggacctgcaa gctgccactt 72780 tgttccattt tgactaataa cataatcagc agtcgtatct ttaccactgc gttttttacc 72840 cacaatcgca attaatttca tacgtttctc atagatggat gcattgactt agacctgatt 72900 tcctgttgca ttgctgcatt actatcaatc tgtggtctac cttctattat tataccatca 72960 atttctacag caaaagactt aaagttgaaa gttgctgttg cgttgatagc ggggtcagaa 73020 tcttctttat atgaatactg aatctctgac aggtcggacg gccatgctcc atagtaatgg 73080 attgacatga caattttgtc cttgttgtta tctaagatat gaagcgtaac cgcttcagga 73140 cgaccctttg gatgccatgc ttttgattcg tgtgtgatgt agttgttgag cgagagcatc 73200 catttataga tatcaagcca tgctcttaag tctttgtcgc acagaaagtt aacaatcaac 73260 gggtcgaatt ctattgtact accaggaaga ctggagcgat ttataccttg tgttccccca 73320 gggacatccg tcacaggaat gtgaactcct ggaagggtaa cgtcttgaac gttgagttta 73380 aaggtctgtg tgaggtttga atcgcttacc tctaatataa agttagtcgt gttagtttga 73440 ttatataaac ctgacatagt gcaccttgtt tgctttaaaa caatcagtgt tataatggtt 73500 ctagaccttt tgtccttacc aataacactg accgataata gattatgaat ttctccaaac 73560 tttttgtgca gagaattttt tgcctttgct gtaaaaagct tgcaacggaa gcataacaac 73620 gtttgcccag tcactgggct tgatttcagt gatagtacct tttatattgc caggtatata 73680 tgctttaatc atctggtcag cccctttaaa tcccttcacc tttgaccaat caattttcag 73740 atgagtcttg ttcgtaatcg taggagtatt agcatattgc ttaagaagtt cctcaaggaa 73800 ctcttgacgc gccttcggcg gaatatagtg caagttcaaa ccatacatca aattgtgctt 73860 acctagccca agataaatga ttaacggata tttgtcccag tacggaagag tatccttatg 73920 ctttgcatca tagatataag cataaatttt acccggttga ggtttagccg ttacgtggcc 73980 acgaactgat ttcttaattg tctcagcgaa ccatctggca gatttggtgt tgcttgctgc 74040 accttcgtta gcaatcttat ctcttatgct ctttctaaag ctattcacca ttatcagctg 74100 gcgttcagcc ttcgtaagtt taccagcagg tttctttttg agtttggata ctttataagc 74160 agtctgaatt ctcgaattgt atcttgacat gcttttggta aaggtcgaat agttgattcc 74220 tttctcttca gcaaacttct tcgaagtcat tcccttttct tttgctgtat aaaattcaac 74280 ccctatctca acccattgtt gttcggattt actgggtttt ggacctggtt tatctgcaga 74340 ttcattgata aatgtaaaaa tactcatcct ttccatccta attttttcag tgagttttca 74400 gtgattatcc tgaacgttat acccatctta tccgcggtag cttgtgcagc tttccatttg 74460 tcagagttaa cgctccaggt gtacatttcg ttaataaatc tctttttact cgcagtggta 74520 ttattggccg gtttggttgg gggtcttgtt tcttttaatg gcttaacttc gaaaaagaac 74580 tgctgtcctg tatcgaactt aacccagaaa tccatataat atctgcgctt tttaccttca 74640 gcattgcaga agtatggaat aatcacagtc tcagagcccc attgaacaac gttcggatgg 74700 ttgtcgagcc atctcatcaa ccaagcctcc cagcttgacc ggtattgtat tttcttccag 74760 tcgcccttat atttttctat gttggtgggc ttgaatttcc cggagtatgc catatagcct 74820 cctaataaat attgatatac ttatttataa atggaggctt tatgcttttt tcatttttta 74880 accctattga ttatgatgcg aaaacaatca gaaaaaactc ggcatcattg acgaaagaag 74940 atgtcttaag cattccaatg actgacatct tcagaaacta ccgcgcttat ttcgataagg 75000 tcgctgctaa ttatacaccc aaaacctatt acattgcagg agctccgaga cccgaagaat 75060 tggcatatca gctctatggt aacacacaac tctattgggt tctcctgatg attaatgaca 75120 tctatgaccc ttattatggt tggcttggtt ctcaggaatc atcctatcag gcatctattc 75180 agaaatattc gaatgtcggt ggtaaccagg tcctttatca tgtgaatgaa gatggtgaga 75240 agttctggaa ccttgtatct tatcctgata atccatacac ctggtacgat aaaggtgata 75300 agaacaaatt atacccgcag tatgaaggtc cattagctgc agtggatatc tatgaagatg 75360 ctatccgtaa aaatgaatac aaacgcgaaa taaaaataat tgacccggaa gacattgagt 75420 catttatttc cgcgcttatc cgtgaaatgg agaaggcttt atgattaata tgtcagataa 75480 tgtaagttgg ttcgtcgggg ttgtcgaaga ccgaatggac ccacttcagc aaggtcgtgt 75540 gcgtgttcgt gtatggggta tgcatccata tgagaaagca caagggcctg ttaaaggtat 75600 cagaaccgaa gaccttcctt ggatgtctgt attgatgccg acatcatctg catcagtttc 75660 tgggattcaa actgccatga ctggtatggt cccaggtact caagtatatg gtcacttttt 75720 agacaagtgg aagctcaacg gtcttgttat tggtacgtat ggttcagcct caagacaaaa 75780 ggcaaatcct aacgaaggct tctctgaccc tacaggacaa tacccgcttt atgttggtaa 75840 tgatgctgca gcgcttaacc gtggcggtga agtcggttat gatgcaactt cgaacgtaat 75900 tcaggatgcg aaccttgacc ttggtattaa cccagatggt cttgatttga gccaggttaa 75960 gcctgatgat aatcctaatt tcacaattga aaatatgctc caccgtgatg aaggtcttcg 76020 actgaaggta tattgggaca ccgaaggtta tcctactata ggcataggac acttaattgc 76080 accacaacct attagagata tgaatcagat aaacaaaata ttatctaatc aaataggccg 76140 tgaagttaaa ggtaaccctg gtgcaatatc aatggacgag gcttctaagt tattccaaga 76200 agaccttaaa aaagtccaaa acgatatagg taggcatagt gttgtgggtc cggtttataa 76260 taaagaaaac cgttcgagac aaatggctct tgaaaacatg gctttccaaa tgggattagg 76320 tggattggct aaattccgtg gaatgcttag cgcaatgtta gtaggtgatt ataaaaaagc 76380 atttgaggaa gctcgtaatt cagtatggtt taaccaaact aaaggacgtg catcaagggt 76440 atcaatgatt atccttaccg gtaatatgga atcatatggt attatggctc ctaaagaaag 76500 gtcatttaaa gggcgttcat ataggatgat tcaaaccttt gcagcccctg caaattctga 76560 cccagccgac ccatggactc cagaagatac acggattcta tttaaagagc cagaatcaag 76620 ttacaagggc gaatatcctt acgtgcagac aatgcaaact gaaggcggtc atattcagga 76680 attcgataac acccctggac aagaacgtta tcgactgatt cacccaaccg gttcctacga 76740 ggaagttgca ccagatggtc gtaagacttc gaagacagtg gccgatggtt attatatgac 76800 ccagggtgat tcgagtactt acgttggtgg taataataaa gtcaacgttg gcggtgatga 76860 aacctattat aatatggcta atgttcgtcg tcagaccgat ggaaacgaaa gcattcatat 76920 tcgtgggaat gaaaccaaaa ctgtagaagg taatggaact ttgcttgtta aaggaaatgt 76980 taaaatagtt attatgggta ctgctgatat aactgttgaa caagaagcat atattacggt 77040 tgaaaaagat acaacagtta atgtcaacca gaatgcagat ataagtgtta aacaaaaggc 77100 cacaatcaat gctcaaaata tggatgtaaa tgtcgaacaa gatttaaatt ttgcagcgac 77160 gaacattaat ttaaacgcat caaatactgt gcatatggat ggtggttcat tagctaaagt 77220 atctggtggc aacgtacagg tcggttaata tgaattacta cgataaagtt gatgaagtgg 77280 tagggactgt aacaagtccc tgtgatgacg cagtaggacc cggtgaatat cgccgggtta 77340 aatgggaatt atataaccca acaggtgctt ttattcaacc tgggaatggc gttgaatggg 77400 ataccgagat tattccacta gctacacatt tatacacaca aaatccattt ggtaataagt 77460 ccgaaacttt tcctgcacct ccgccgccgg aagatggaac aagtcaaggt atctggtgtg 77520 ctaaactcgg aatattcttt attacgcaat tggatttaga aaattatgta gaatcgattg 77580 gtgcaactct tttgtccggt aatgattggt acggtgctga tttatcaaaa tccgtattgt 77640 ataaaacatc tattgaatat gatatgtatc gcatcgaaaa ctgtggcggt gtatggacgt 77700 cacaaggccc tagaatggac cccaggacga tgattgcagt tacggctgat tatcatatgt 77760 atgtcaactt taacggcctt ggtgactccc gcagggctta tttgaagtac tatgagacaa 77820 aaccttgtac ttatgttgga ctggtattaa atctagatgt tgatatgagt atcccaggaa 77880 ctccgattcc gggaccggta tccattgggt ctttaccgcc agaaataacc gggcgagcca 77940 gggcaataag ctgtgttcct tttacagcgg aattccctta caacggattg accaatgcga 78000 ttatggcttc atggaataca tctaggaatt cattcaaatt atattgggat atgctccctg 78060 gatggccatc atctggtgga accgattggg aaaaattctt cgattctggt tatagtaagc 78120 tatcggtcgc aatggaagag ctaggcaact cagctgttgc tatgtgtcaa tatgttcagc 78180 aaatggcttt tcttgcattt aaagatgcca tatcacaggt cttgaatatt gttggcggtg 78240 ggtgggactt acttaaaagc tttttaccct cgattactat tatgggcatg actattgacc 78300 tggaagacct ctgcacttca ggtgatggtg tacagaaatt aaaagaagca tggaaaaact 78360 ttaatttaga agaaaccatc caatcgattt attctgccat tgggtcggca tacgattatt 78420 cggttgaacg cgtcaagatg tattcgcgtg accttattga tgcaattaca gacttgtatg 78480 actgggcctg gtctcaattg cttatggctg gtgttgcatt atcaaaactg tgtgtagacc 78540 tcgctcaaat atggagtatg cctcctatag ttccgaaccc agtttggagc gttattactg 78600 cggtcaagga aatgatgaag caagttaaac cactggacat gataatgagc ggtaactttc 78660 caggattcac agcatcagat gtttatcaaa tggttatgga aaaggtagac cagaaaatag 78720 acgaagctta tgcaatgatt gaggctctta aaaccaaggc actcaatctg tggaatgaga 78780 ttaagctcaa aaagcaagaa gtacaaaaac aagctattga atttaaacaa tatctttcag 78840 gaatgtacga aaaagttaca gacgagctaa ctgctcagaa ggaagccgca ttatcacaag 78900 ctgaagctgc acttgaactt ttaaagaata aatataatca gattaagaac tctattaccg 78960 aaagtcaaac ctctgtttct gatgtattag atatggcttt agaagaattc aaaaaacttc 79020 ctattgtggc tcaaatggaa gaacttctcg gtatgctcgg agctactgta gatgatatca 79080 tggttatata cgagaactca gtaactaaag caaaatcttt atatcacgaa tttactgatg 79140 gttccaggtc aatgaaagac ttgtgcaaat caatgtataa ccaaatatca acattatcct 79200 taagtaaggt ggtacaatgg attaataaga tgctgggaat attcggttta acgattgcat 79260 ttccatccct ttccatttgc ataccttgtt tagaatctcc tactaggaat taaaatggct 79320 actcctacga attaccaatt aacaagaacc gttaacgcaa tcccaaaagt atttgtggga 79380 gctacgtttg aagaaattaa aaagaacatc attgactggt tatcaagcca ggatgaattt 79440 aaggattatg atttcgtagg ttcgcgaatg aatatcctga ttgatatgct tgcatacaat 79500 acattgtata tgcaacagtt tgctaacacg gcagtttacg aatcatttat cggaactgca 79560 aacctaaggt cttcggttgt tcaagctgca caggataatg gttatcttcc atcttcgcgt 79620 tcggccgcaa agacttcggt tatgctgaca tgttctcacg ctctccaacc atcgaatatt 79680 gaaatcccgc gtgggactaa gttcctggct tatgctcgtg atacatcagc tgacccttat 79740 atgtttgtca caacggataa tgtaatcgcg gtaaaagaca ttaacaacca atatctgccg 79800 attgtatccc ttgcacaagg tcgtattatt cgtactgaac ttttgtatga cccgaaacaa 79860 cctattctta ttcgtgaccc ggatattgac cgaaaagaag ttaagctttt tgtgaacggt 79920 atcgagtgga ctgactggac aaacaaatca atggttcatg ctggctcaac gtccaccatt 79980 ttctatatgc gtgaaactgt tgatggtaat accgaattct tcttcggtga aggtgaatcg 80040 accaaatctg ttgccggtgg cgtattggaa gccaactata ttggtggatt gaaacccgta 80100 caggattcga caattgttat cgaatatctt cgcacatcag gggccgatgc aaatggtgca 80160 gttgatttca gctatgcaga ctccttgact tatatcactg ttgacaagat tacagaaaac 80220 tacaataacg ataaagatta cgttggagct gatggcggtg gtgacccgga agatattgaa 80280 cgaatccgtg aactggctgt agttaaacgt gaagcgcaga tgcgtgctgt caccgggacc 80340 gattatgata cattcgtatc agaaagattc ggctcaatcg tacaagccgt acagacgttt 80400 acagacccaa ataaacctgg atatgcgttt atatcagtta aacctaaatc gggcttgtat 80460 ctcaccgcag tgcagcgtga ggacatcaaa aactacctta gtgagttcaa cgtatcgaca 80520 attacaccat cagttatttc tcctgattat ttgttcctta aacacaatat caaagtgtct 80580 tatgcgctga ataaattaca ggaatctgaa cagtggttgc aagcacaaat cattaaccag 80640 attgaccgct attatattga tgaagttgaa ttgtttaacc acagttttgc taaatcgaaa 80700 ctgctcactt atgtagataa taccgaccat agtattttgg gttcgtctgc aacggtgaca 80760 atggttcgcg aggtcttgaa cttctttaag actccagaat ccggcattaa atattataat 80820 caatatacta ataggtcggt ggaatcaagc gaatttgaat tcgttccaac aaaccaaagc 80880 gataccgtta cctcatataa cgtccgtatt gtagcgactg accgcaactc atctggtgat 80940 ggtaaaatgg tcattggtcc gttccgtcca ggtgatgttc agcttaatgc gaatattcaa 81000 ccttacacag gtaatgactt taataagatg gttgcaccag ctgaccagac tcaatacttt 81060 gtaatcggtg aaatcgatta ttggtctgat tatatcttct gggacattgc cgcgattggt 81120 ttgacttcgg accgttttga agtgcaatca atcgaactgt atgcgggtcc agaccaggac 81180 aacatctaca caaaagacgg ttcgcttatc gtattcgaaa atgaccttcg tccacagtat 81240 acgactatta atttagaacc aattacatta taagcctctt cggaggcttt aaggaatctt 81300 aatgactgtt aaagcccctt ctgttactag cttgcggatt gataaactat ctgctaacca 81360 ggttagtatt aaatgggatg atgtaggctc caacttttac tattttgtgg agattgccca 81420 aatcacagaa aatggagtac gcatcccaac tagagattta atttggcgcg accttggtta 81480 taccgcagac aatgaatggt tcgagcagga atatattcgt ccgttgacaa gttatcagat 81540 gcgtgtgtct gttgcggcaa tgggcttcga acggtctgat tgggaatata ccgaggaatt 81600 tgaaaccttt gcaacgaacg catatacttt tgaacatatg cgcgaattta gtttggccaa 81660 taaattcatt gaagaaaaat tcaccaataa aaatcagagc tatatcaact ttaacaatga 81720 tattattatg gcttcattga tgtccgaaga ctttacatgg acttctgctt attctcattt 81780 gtcttcaatt tcgaactatg ttttgcaaga agaccaatat catgaaatcc agggaccgat 81840 tcaagcagtt tgtgttgacc aaaaccgtac gatgcttgct gaacttggtg gcgtgcttta 81900 tctgttcgaa cgctttcagc cgatggttaa agtctccaat gacaaaggtc agaactggta 81960 ttatataaga ctctttaatg agcgtatcgg taaccctgtg tcaaggactg tatattatca 82020 gactgatacc acaacttacg ttctgggcta tgaccgcata ttctatggtc gccaatcaag 82080 cgatataaga tggtctgcgg atgatgttcg attcagttct gaagacatta catttgctaa 82140 agtcggttca gatattaacc ttggatttga tattgaaatc ttcgggaact atgcaagact 82200 tccggcctat atttcacaaa tagctgaagc tatcacggtt tcggatgaat tcatttatgt 82260 tgcggcgcgc ggtcgtgtat ataaagcaaa aacctctgat gcaccgattg atacaactcc 82320 agggtctccg accttcggtg aaaaactatt tgaaactggt tacagtacaa ttacgacaaa 82380 ccctaaagca gtttgttata aaatggattc agttcaaggt catacgtttg cattaattac 82440 gggtgaagtt aaagaagaaa gacaagaccc aactaaacct gaaaacgttg ttgactctct 82500 ggataaaggc gtttataaat tagaccccga tactgataca tggacgagag tttttggcaa 82560 tacagaagaa gaacgtcgtc gaatcgaaca tggttatgcg agcatgtcaa ctgatggcat 82620 cgaagtgttc tttagctcca gtaacttcaa atattcggtt gagcctgata ctgaactacc 82680 tcttaaatat cctgatgaag ttaatgaagc agtcaaatac gtttacgatg agcaatggat 82740 acatgataag cattacctga tgatgagctt cagagctggc gaagattctg gcttcgaaga 82800 gttcaaacca ggccgtatga cttactatgc agagccgttc tttaactggt caagacgtga 82860 cgatactcgt tgttggatta cgacttcaga ccgagcaatg gttgtctata acgatattct 82920 ttatcagtac atcatcgata catttggtgg aacttccccg gaacgtatcg tgcatgaata 82980 ttggaacaaa ggtcgagtaa cgattactgc acctaacatc gaattcaacg gctttaaaaa 83040 atatgcgtcc gggattatgc ttcataaaaa taccggggaa ttaattggct attttgaatt 83100 tgactatcgc gtgcgtgatg aagtgcgtat catctggaag ccaaaagaaa ttatgcttat 83160 ggctgaacta caaaatcagg aacatgatat tccgtggact ccggaaaatg agaataagga 83220 aaaagacccg gacttgcaac cactgttaac gaagatgatt ccagatagtt atcttttaca 83280 ggatagtaaa tttgaaaaat tctgtgaata ctatttgcag ttcatcagtg atggttccgg 83340 tacatattat aacaacctgt tgaatcttat tcgtaatcaa tatccgcgcg aggaagatgc 83400 atgggaatat ctctggagcg aaatgtacaa acgcaacatt tacctctcca aggaaaaacg 83460 tgacgaagct gttcgattct tccaggcccg acagtctgat ttttgggcaa caaaaggtac 83520 agaagcgtct tataaattcc tgttcaaatt actgtataat gaagatgttg aaattgacat 83580 cgaatccaag aacggaaccg agtatgatat cgtagttgaa tctgataata tcagtgaaga 83640 cattgtaggc caaaccattt ataccgcaac tgggcgcagt aatgtgactt atatggagcg 83700 cgattataaa gatggtaaat taagatggcg tattactatt cataacctcc tgggacgttt 83760 tattgttgga caagaaatta aatcagaaag aacctcattc gaggggatga ttgtacaagg 83820 tgttcgtggt aaagagttgt tgagtaacaa catcgattat attaaccgta atcgctctta 83880 ttacgtaatg acaattaagt caaaccttcc gacctcccgt tatcgtaatg acgttttgag 83940 attcgttcac ccggttggat ttggattcat cggtatcacg ttgctgacaa tgtttgttaa 84000 cgttggtttg actcttaaac ataccgagac aatcattaat aaattaagta actatagatg 84060 gtcttctggt attccatcag aatggaatga ccgagttgcg gtgattggtg ttgatggcac 84120 tattgagaaa aaccctatca caggtgaagc aatatataac atcgctccta atgctggcca 84180 gcctttcccg ttaccagccg attatgacgc cgagaacgat ttctctatgt tctatggatt 84240 gttaccgtca caacgtcgtg gaaaatatag tccactgttt gaccagtcag ctgttacatt 84300 ctcgaactgg agagcattag ttgaccaacg tcttaaagat gatattggta atccaagaga 84360 cccggttaat cctacacagg taaatgtaaa tgaataactc taccgtagtt taccgttcga 84420 ttgttacatc aaaattcaga acggaaaaaa tgtataactt ctacagcgtc atcggagatg 84480 gcgccaatga aaatacaatg tatgtcacgt tcggtcgtgc agaaccatgg gctgcaaacg 84540 agaacgaccc gggctttgct ccaccttatc cggttgatga tgcccaaggt gttgaagatg 84600 tttggactaa tatgatgggt gcagtcaagg tgtataaatc catgcttgac tgcgtggttc 84660 ctcgtaagga ttggggtgat gttcgttatc ctaacccgct gaactttcag attggcgaaa 84720 tcgtcgtaag taataccgca ccatataacc gaactgatgt tggtaaaggt tggatggttt 84780 atcgttgtgt ggatgttcct gaatctggga cctgttcgat ttattccatc actaataagc 84840 aagaatgtct taagcttggt ggtaaatgga cttcagacta tgcttccagc cgagcaccat 84900 cagggactgg tgatactgaa ggacgtgttg atatgggtga tggttatctc tgggaatatc 84960 tctatgagat tccacctgac gtatccatta accgttgtac aaacgaatat atcgttgtcc 85020 catggccaga agaaattgca gaagaccctg aacgttgggg atacaaagat aacctgacat 85080 ggcagcaaga tgattatggt ttaatctatc gtatcaaggg aaatacaatt cgattcaagg 85140 ctttcatgga ttcgatttat ttccctcagt tcagtcttcc aggtaacaaa ggttttagac 85200 aaatttcaat tgtcgctaac cctctggaaa ttaaagctgc tcctaatgac ccgaatatca 85260 aagcaacaaa agaatattac gatgctattg atttgagtcg acattctggt gaaatgattt 85320 acatggaaaa cagaccacct gtttatcgtg cgatggacca aaccgaagaa gtaaatatta 85380 tttttgaatt ctaactaggg accttcgggt ccctttttat tttataaata tagctatatc 85440 aataatgagg cttacctatg attaatcaaa ccccaaaact tttaatagat gtcggcgaaa 85500 ttggtaacgc cagcaccggc gatatcttat atgatggtgg tcgtaaaatt aacgaagatt 85560 ttaataacgt ctataatacc ttcggtgacc aacgtcttta ttcaatcgat aacggccaga 85620 actctcaggt cttgcatgct acaggttatt atcaaaaagc aacaaacata atcgaatgga 85680 acactcctgt tgcgcttggt tctctccgcg atgtcgatac ctccaagggt gttgttactg 85740 ccaggattgc ccgcggtaaa atgggtgaag gtattatctt cattaactct aacgggactt 85800 tatcaccaac tcaatctctg gaaatcgaac ttcaaggcac agatactttc gtttcggctc 85860 cgaccaacaa aattcgcttc acaagccctt atacaaagat tactctttgg tgtatcgatg 85920 acgcaaacgg cgttggcacc tgggattatt caatcgaaaa tatgtttggt gtaaaacata 85980 ttcctttgga ccgaacttat aatatcggtc tgaacgcaag agatattccg attgcattgc 86040 gcggtgattt caaaacaatg aaacttttgt taaccgctat atctactgat gaaactaaat 86100 acaaagcatc agaggtcatg ctctttgtag acactcaagc taaccaagtg tatagcacag 86160 aatacggtgt attgcgtcgt ggtcacgtgg atgacgatga tgagttatat accattaact 86220 ttaaaatagg cgctgacaac ttcgttacgg cgaccgtaag cgcaccagtc tccatgaagc 86280 tcgccattaa ggtagccgag acacaaacca ttggggtagc aatctaatga aacaaaattt 86340 aaaaataggc gtgatggtcg atgatggtac cggcgattat cttcgtctcg gcggccaaaa 86400 attaataata actttgatga cctttactac cagctcggtg atggagaaaa tcctcatgct 86460 gctggtgcat ggaaaacatg gtcagtagcc gacggtacaa ctctggaagc cgttatgggt 86520 cgtagctatg cagtgaatac ctcaaatggt atgattactg ttaagcttcc taaaggaact 86580 ccgtctgaat ataattacgt tattcgtttg cgtgatgtgt ttgcaacttg gcaagtctcc 86640 ccggtgacga ttgttccggc tgttggcgac acaatgaaag gttctcctga ccctgttgtt 86700 atccgtcgta acctggcaga tatcgaactg gtttattgct ctcctggccg ttgggaatat 86760 attgacaaca aacagattga caagatttcg aataacgacg tggcaactgt tgagacccag 86820 caattcatcg caacagaagg tcaaacggac ttcctggatg tgtttaatgg tcgtccttat 86880 aacattgcaa gtctcgaagt taaacatcgt ggtaacgacc tgttttatgg caaagatgat 86940 attttcgacc cggtgaatgc tgaattcggt tcgcctggtg cagcgcctgg tgaaattgta 87000 acccttgacg gtcgcaatat tcgtttacgt atgccttgtg cagctggtga cacggttatt 87060 gtcagaacat tcaacgatgg tattgctcag tatcgtagtt cttataaccg tcgagatgtt 87120 caaatccgtg attcgaaatt tactcttgaa accccggtcg aaggctcttt ggttgtagct 87180 gatttatcta ctaaacacac atttatggtt actgaacttg cggtatctcc tagcagtcca 87240 attaacccga atgcatgtga agtttatatc aacagcatat tacagcacga agcaggaact 87300 gcaaactatc cagcttatac atgcgaaggt gctaatggtg acactgcaga agagtgtaca 87360 gataatggtg gtcaatgggc taaatcaaaa gaagactata taacaacctt tgatgaagac 87420 aaccgtattg aatcttttga gtttgctcgc acatttgaag atggtgatat tctgtctatc 87480 gtttggtata ataacgatat tggcaccaca atgactcttg atgaaattac agatgaaaca 87540 aataacatct atgtttccca gggtccagaa gtttcaatca ccggtgatat aagaattacg 87600 gattacgata atccatttgc tcctaacctg gaaccggttg acccgagcga aattaaacca 87660 tctgctgttt cgattctctt taatatgttc catccaattg gcacaattta cgagaatacg 87720 actaacccaa ataaccctgc gacatatatg ggtatgggaa catggaagcg acttgaagag 87780 cgtgttttga ttggttggtc tgctgaccca gcctcaaact tccatacaaa caataacgac 87840 ctggattctt ctggtgttcc gcaggcaacc gcagggggta ctggtggtaa tgtacaaatt 87900 cacattaaac caaccaatat tcctaaaatt cagaccgatg aaaaagtatt ggtagctgat 87960 gcaactggcc caattattgt tggtggttgc ctgattgacc cggatgcaca aggtcctgct 88020 tatacaaact atcgcgaaga ttatgcgact attaatgaag ctcttgaccc actggcacag 88080 ccaatagata cgcttcctcc atatttgatt gtttaccgtt gggtaaggat tgcataatga 88140 ctacgttgac aaataaaaag gcgagtgtaa tatctcgcca tgctggttac ctccagtttg 88200 attataccac taccgattcc actgctgtag cgggtaaccg ctatatcggt ggtccatctg 88260 ctaaccagac aaatgacggt gtattctatc cgacagtcca gagcgcaatt gatgatattg 88320 catttcgtgc agaacttcct gtcgggtcga taattactcg tcatgagaat actgctcctg 88380 gatttattag tcagtcagat aagattaagt tcaccgggtc tatttctggc gaaaaccttg 88440 gcgaaacggt tcttattaaa gtattcggtt tcccggtaga agttattgtc ggtgactcga 88500 gcgtgctggt tgcatcgaaa gtcaatgacg ttcttttgac tgcaatttca cagggaatga 88560 ttatttctgc gactgctatc gatgcactgg accagtctat attaaatatt acatataacg 88620 actatcagaa tcacatcttt gctcccatga ctcaatatgg ttgtgttatg gaacaaacta 88680 ttgttcaaca accacgtgcc gggtatgggc aatgggaata tcttggttta caagatgtaa 88740 cattgcaagg cggttcggtt aatggtccaa catctcttta ctactataag cgagtctact 88800 aatgacaacg actaacacgc taaaacatat tagcgataaa tccgaatata aaacatttga 88860 cccggtggga acttcatttc ctccgtcggt aacaaatgtt caggaagcat tgggagcttt 88920 ggctcctatt gctattgatg gatttatccc agaatctacg gttgcagtac caggtattat 88980 tcgcctggcg accgaacaag aagttattga tggcataggc aataacactg cggtaacccc 89040 cgaaactctt aaagctcgtt tgaatattcc aactcaggcc acagagactg ttgtcggaat 89100 tacccgttac gcaaccgatg tagaagctat tgatggcaca gtaaccgatg cagctattgt 89160 cccatcaagt cttaaagctt ctattgataa tacatttaat acccgcactt ctacagaaga 89220 ccgtaatggt gtaattaaga tttcgactca agccgctgct ctggcaggaa cagatgatac 89280 aacggcaatg actcctttga aggtttcatt ggccatcggt gcagcaactg cagctcttcc 89340 aacttatagc acagcaacaa ccactgtaca ggggattgtt aaaattgcaa ccccagccga 89400 ggttcaagcc ggtgcaatga ataacggtgt ggcagtttct ccacttggac ttatttcatt 89460 gactgcaaat gcctctcgca gagggttggt ccaattggct tccagtgctg aagcaattac 89520 aggtacggat gcaacaaaag caattacccc acaagctctt caggcaaaaa ctggtaatac 89580 cggacgactt ggtattgtca aactgacaac taccgttggt tcaggggatg gtaacactgc 89640 tcttgcatat aatgcaaacg taatttcaac cactggtggt acaattactg gaactcttaa 89700 cgtcaacgga actttacgta gaaatggcca ggacgttgta acccgcaatg aaattaaaga 89760 tgatgttcca atcggaacta ttattatgtg gggtggacag acaggaaata ttccttctgg 89820 ttgggaaatt tgtgacggtg gtaatgtcgg tgatgctggt ttccgaaatg tcgttggtag 89880 taaatggggc gcaactggta ataagccaga ttttcgtgga ttattccctc gcggttccaa 89940 tagctctaac gatggcgcta tgaaggaatg ggaaacaaac attaatataa gaaacgccaa 90000 gggaaatgat tccaaacaaa aacctaaatt gggtcaaggt gttggttcat atggccatgg 90060 tacagtgcag gcacaacaac tccgtttcca caagcacgca ggtgggtttg gtgaattttc 90120 tgatgacggc tcttttggta atagcatacg ttctaatttt gtagggacta gcaaaaaaat 90180 agactgggat aaccgctcat attataccaa cgagggatat gaaatagatg gtaataatcg 90240 cacccctaaa agtacattaa acagcgaagg tctgataggt aatgaaagcc gcccatggtc 90300 catgtccatt ctttttatta ttaaggtcag ataaggaata aaaatgatta acaaattaga 90360 attagcgaat cttccgtttg tcgatggtgt accagaagct gaccagaaac gaattgaatg 90420 gattcgcaat ggtgattgct taactgcagc cactaccaaa tatgggcacg atggtgccct 90480 taacgccgca gcttttggcg tacagaccaa cgttgaaact ctggaaaaga atgacacaac 90540 cactaaggac aaagtaaacg aaattgtcga tagtgttaac cagattaaag aagctctgga 90600 tatttccact gatgcaagcg ttatccaaca gattgaaacc aaccgtctta atattgtaag 90660 tctccagacg ggtttagccg aaactgatat tgctttactt gataccagca caaatctcgg 90720 tgaattacag gaagatgttg gttattataa cccactggaa gatggaccat atttgaccgt 90780 tcgtgctaat attaaccgta ttaagcatga aatgggccat tatgcaaacc aagatatcaa 90840 tggtgaccca ttgcctgaag gcgaaactaa tgatgccacc gggatgaaac gtattatcat 90900 cgataacagt acagcaattg caactacgca acgtcgtctt actattctcg aggaaaatta 90960 caatgattct gatgtaggtg ggctcacgat taaattaaat gagatacgcg aagaattagg 91020 tccgcgtaca gaaggtattg gtaaatctcc tgtttatact cgtttaactg cagtcgaagc 91080 taaagccgga actaatgaaa ctgatatagg gaatatcaaa accgcaattg gttttggcca 91140 aggtcaaagt atcgcaaacc gtgtcgggac tctggaaacc aaggcaacaa atttagaaaa 91200 taccacttct ggtatgaata caagattgtc gaaagttgaa acagatattg gtactgatgc 91260 cgaaccgact tccattaatg gtcgtctaaa aacgttgcgt gatgacatga caggtgttcg 91320 tgttgttatc ggggaaaata caagcagtgg tttacaggga caagttgttg cccttaacgt 91380 tgcagtaggt attagtgata gcccggcacc gacttcctta attcctcgta tgcgtgaagt 91440 cgaaacttct agtcgtgcga acgctgcagc tattcaggat attcaggctg aaatcggtaa 91500 caatcagtca ggcatcaaag gttctatcct gactattaaa tcacaaatcg atgggaccaa 91560 cccaaacggt tcgactgttg ctgaaaaagg tattctcccg actgttgaag gtctggtcaa 91620 tgaaatgctg actaagctca gtgatgcacc atctgatacg aagatttacg gtcgccaaga 91680 cggtgcttgg gccgaaatcg ttggtggtga tattcctgct ctagtatcac gtgtggatgg 91740 tatcgaatcg cgcaccagtg gagccgagat actgataact gaacttgaag gtaaagtatc 91800 agccttagaa acagagctcg ccaaaaggcc tcctgtcgct cctacgactg acggacttcc 91860 ttatgtgctc gttgataatg catgggttcc tttgtcgaca ttcgtgactg ttggaccata 91920 acaaaagggc ttcggccctt ttttcgtata aatacagtaa agaggaaaac atatggcaac 91980 tgaatcatac aacccgaaac aattaaaaga tgcaatcctt cgtcgtcttg gtgctccaat 92040 cacgaatatc gaagttacga ccgaacaggt ttatgattgt atccagcgcg cattagaatt 92100 atatggcgaa tatcattata atggctataa caaaggttat caggcttttt atatcggtca 92160 tgatgaagtt tcacaacaga aattccgtga tggtgttttc gatttaaaag gtcgtaatat 92220 ttttgcagta acgcagatta ttcgtactaa cgtcggttca ttaacttcga tggatggtca 92280 agcaacatat ccatggttca cagatttcgt tctggggctg gctggtatta atggtggaat 92340 gggtagttcg tgtaatagtt ttggtccaaa tgcttttggg gcagaccttg gttattttac 92400 acagcttatg caatatcgtt cgatgatgca agacctgatg gttccacttc cagattactg 92460 gtataatgac gatacggaac aacttaaaat tatgggcaac ttccgtcagg gagatgttat 92520 tgtaattgaa gtttacacca aatcctttaa tggtgttgat gcaatggcca gcaatactgc 92580 gggttacggt tatgcttctg gtgattgttc aaatccatgg aatgctggtg cacagtggga 92640 taaccctgca cgcagaataa gcggtatgag agccggtgag gacttcggac ttcaagatgg 92700 cgcatacaac aaccgttggg tgaaagacta tgccacggca ctcgttaagg aagttaacgg 92760 caacgtattg gctaaacacc agggaatgat gatgccaggt ggtgtcacag ttgatggtgt 92820 ccgtttaatc gaagaagctc gcatggaaaa agaacgtctt cgcgaagaac ttgacctgct 92880 ggatgcaccg gctggaatac taattgggtg attaatgact acatactata gactttatgt 92940 atcaacattt gaaacatcta atgggataat ggtatatggc ggtaaacgcc attcacatta 93000 tgaagattct aaagcggata gttatttcgg ttctggtact attgtcaagc gagctaaaca 93060 aaaatacgga aggaaatgtt tagtcgacat tacatggtct aaaccttttg aatcgcctgc 93120 tctattaaaa gaagccgaag ccttactagt cagtgagctt ttagaaagtt tcgataattg 93180 tgcaaattta catccaacag ggggaggtgg accagaaggt tatagttgtc cagaagattc 93240 ccctgccttt ggtaaaaaga gaacagcaga atcaagaaaa cgaatgtcag acgcagcaaa 93300 acaaagggaa tggtcagaag aaggattaaa acgtcgtaaa gaatcaacag ctcgaatgtg 93360 gattgatttt aaggacagac atccagattt ttctgatgat aaaaatccgg ccgcgcgtaa 93420 aattattacc ggcgacctag aattttgtac cggaagaaaa tgtgcagaat attattcgat 93480 aacaccggcg acggtcattt acagatgtaa aagtacagcc gaaaaatgga aattatggaa 93540 ttatgcgtaa ttcttatgat gcaagcttat tcgctcggct cgaagatgga accgggtatg 93600 acaatacaaa ctcgtcagag atactcaacc aatatgtaaa ctttatgcat catgaaaata 93660 cccagacttt agcagatgct attgttgctg aaagtattca gatgcgcgga atgtctctct 93720 attatatccc aagacaattc gttaaaccag atttgttatt cggtgaagac ctccagagta 93780 aatttgataa agcatggaaa tttgctggtt atctggatac gttcgatgct tatcagggtg 93840 atggttctat ctttagtaaa tggggtatgt ctgtgaacga tgaaatcaca atatcgatta 93900 accctaatct gtttaaacac caatgcaatg gaactgaacc tattgccggg gatttgattt 93960 atttccctct tgataacagc ctgttcgaga taacctgggt gcaaccttat gacccgttct 94020 atcaggtagg tcaaaacgcc attcgtaaaa taactgcgag caaatacatt tactccgggg 94080 aagagcttaa acctgaatta caacgaaatg aaggtattaa tatttcagaa ttcagtgagc 94140 tcgatttgca accaatccac aatatcgatg gccttacaga tatcagcgat atcccttatg 94200 cagaatccga tgagattaac gcagaagcgg ctgaatatgt taaaccatat tttgtgcaga 94260 acggtcgtgg acttgagtct cctcctaagg caaaacagga tagctttgac gatagtttct 94320 ttgacgaata aatacattta ttaatgcgcc cgaactgggc gcctggaggt tactttgttt 94380 gggcattttt ataattcgtc tctgcgtcgc tatatcgttt taatgggcga tttattttcc 94440 catgtacaag tggccagaac cagagaagac actggactta aatatattaa ggtccctatt 94500 tcttatgcgt caaaagaaaa attcatgatg cagcttggaa aatggaccgc tattcagaat 94560 acaccgactc ctgacatgtc gccacaagaa cgtgctgctc agaaggctaa aatcgagacc 94620 gtattgcccc gtatgaacct acatatggtt gacttgatgt ataacggaca atataaaacg 94680 gctctccaga atcacacaca ggcgcgattt gctaataacg accctcgcaa gcagatatct 94740 caatattctc ctgtgccggt caaaatgatt ttcgagcttg gaatatatac aaggcatcaa 94800 gatgatatgt tccagattat cgaacaaatc atgccttact tccagccaca ctttaataca 94860 accatcaccg aactttatac gaatgaaatt acgttcgaac gtgatataag aattgtattg 94920 cagtccatag caatggatga gcaaattgaa ggtgatgcaa tgactcgtcg tagactcgaa 94980 tggtccatta tgttcgaagt taacggttgg ttatatccac cggtcaagga agtagccggg 95040 gaaatccgta ctatctatct tgacttcttt gcaaattcca gagagcttgc accggaaggt 95100 aatttcgaat ctgttgactc ggaagccgtt ccacgagacg tcgaccaaga agactggcaa 95160 ggcgaatctg ttcaaaccta ttcgcaggat attcctgtac cggttgaccc agcacctcca 95220 gctccaagaa aaaatagggt accattatga gtgaacttga tattaacaaa ttgatggata 95280 taacagacct tcccgggatt gccggggagg aagttactgc atacgaacca ttagtattaa 95340 aagaagtcga aagtaatcca aataaccgta ctccagacct cgaggacgac tattctgttg 95400 ttcgtaaaaa cttacacttc caacaacaga tgttgatgga tgcgggtaag atattccttg 95460 aagcagctaa gaatgccgaa tcaccgcggc atatggaagt ctttgcaact cttatggggc 95520 aaatgactac cacgaacaaa gagctgttaa aacttcataa agaaatgaaa gaaatcaccg 95580 ctgaacaaat cggcacaaaa ggtggtgcac catcacagac aaatattgaa aatgcaacta 95640 ttttcatggg gtctcctgct gatttgatgg atgaggttgg tgactcatac gaagctcagg 95700 aaagacaaga gaaaataatt aatggaacag ccggttaacg tattaagcga cgaccatccg 95760 ctgaatatag gcgataacat tgcaatccgt cctcccggtt cattggagac aaaagttgag 95820 gacggaatac actggattaa atcgcagtgg gatggtaagt ggtatccgga gaagtttgat 95880 gaatatctta aaattaataa aattgtaaaa attccatcga acgcggataa gccagaatta 95940 tttcaaacct ttaaagataa gaataaccca cgcacccgtt ataatgcgtt tcctaacctg 96000 aagcgtgcaa acataaaaat taaatggacc aaggaaatgg ttcaggagtg gaagaagtgc 96060 agggatgata ttgtatattt tgcagaaacc tattgtgcga ttacccacat tgactacggg 96120 acaatcaaag ttcagctccg tgattatcag cgggatatgc ttgaaatcat gcacaaaaag 96180 cgtatgaccg tttgtaacct tagccgtcag ctggggaaaa caacagtcgt ggcaatattc 96240 cttgctcact ttgtatgctt taacaaggac aaagctgtcg gtattctcgc acacaaaggt 96300 agtatgtcag cagaagtgct agaccgtact aagcaagcta ttgaattact tcctgatttc 96360 cttcaacctg gcatttctga atggaacaaa gggaacatca cccttgacaa tggttctact 96420 atcggtgcat acgcatcatc tcctgatgcc gtccgtggta actcctttgc aatgatttac 96480 atcgatgagt gtgcgtttat tcctaacttc gttgatgcgt ggcttgctat tcagccggtt 96540 atctcatctg gtcgtcgttc taaaattatc attacgacta caccaaatgg acttaaccac 96600 ttctatgata tttggactgc agcaattgag aagaagtcag gcttcgaacc ttatactgca 96660 atatggaact cggttaaaga acgtctttat aatgatgagg atatttttga tgatggctgg 96720 caatggtcga agcagactat cagtgcatct actctcgaac aatttaacca ggaacatggt 96780 gcgcaattcc agggaacttc cggtacatta atctccggga tgaaattggc aataatggat 96840 tggatagaag ttatgcctga aaatggatat ttctatcgat acaaaaagcc agaccctgaa 96900 cgcaaatata ttgcctcact tgactgttca gaaggtcgtg gacaggatta ccatgcattg 96960 cacataattg atgtaaccga tgaaaaatgg gaacaagtgg ctgttttgca ttcaaacgaa 97020 atatcccaca tgatactccc ggacatcgtt tataaatatc taatggaata taatgaggca 97080 cccgtttaca tcgagcttaa cagtacaggg gtttcagtcg ctaagtcttt atatatggat 97140 ttagaatatg aaaacgttat ttgtgattcc atgattgact tagggatgaa acaaacaaga 97200 agaacaaaac ctgtcggttg ttcgacgtta aaagacctta ttgaaaaaga caaattaaaa 97260 attaatcaca aacctactat tcttgaattc cgaaccttta gccaaaacaa attatcgtgg 97320 gcggctgaag atggttttca tgatgacctt gtgatgagtt tagtgatttt tgcctggtta 97380 acgacccagc aaaaatttgc cgattacgtt gacaaagaag accttcgttt agcatctgaa 97440 gtattcggtc gcgagttgga agacatgaat gatgattatg ctcctgttgt attcgttgac 97500 tctggagacc tttcggccga atatgcacct gcacacggtt tatcattcat ataaataaag 97560 taaagcaaac tattagagga ctcaaaatgg ctttattatc cccgggcatt gagctcaaag 97620 aaacaacggt ccaaagcact gttgttcgta acgcgacggg tcgtgcggcg ctggttggta 97680 aattccagtg gggcccagct ttccaggtaa ctcagattac aaacgaagta gaacttgttg 97740 atttgtttgg tggcccgaac aatatcacgg ctgactactt tatgagtgga atgaacttcc 97800 ttcaatatgg taatgacctg cgtactgttc gtgtagttaa ccgcgaccac gctaaaaacg 97860 catccccggt agcagggaac attgaaagta caatcgctac tgctggttct aactatgcag 97920 tcggtgacgc tatccaggtt aaatacaacc agactgttgt tgaaacttct ggccgtatca 97980 ccaaggttga cgttgacggg aaaatcctcg cggtcttcat tccgtctgac aaaattattt 98040 cctttgcaaa atctgtaaac cagtatcccg ctttaggctc ggcttggact gctgaaatcc 98100 tgaccacttc ttcaggtgtg tctggcacga ttaccctcgg gaaaattgtt accgattctg 98160 gtatcctgtt gaccgaagca gaaaatagtg aagaagctat tacctctctg gaattccagg 98220 cttccctgca gaaatatgca atgccgggtg ttgttgctct ttatcctggt gaaatcggtt 98280 cgactctcga agttgaaatc gtttccaaag ctgcttatga tgctggctct tcaaagatgc 98340 ttgacatcta tccgaatggc ggttcccgtg cttctgtagc tcgtgcagtg tttaactatg 98400 gcccacagtc tgacgaccag tatgcaatca tcgttcgccg tgatggtgat atcgttgaaa 98460 acgtcgttct gtcaactaaa gaaggcgata aagacgttta cggcaacaac atttatctgg 98520 atgactactt tgcaaaaggt acttccaatt acatctatgc aacttctctg aactggccaa 98580 aaggttttgc aggcatcatc aatctgatgg gcggtatttc tgcaaacgac caggtgaccg 98640 ctggtgattt gatgcaaggt tgggacctgt ttgctgaccg tgaagcactg catattaacc 98700 tgcttattgc tggtgcttgt gctggtgaag gtgatgcgac tgcttccact gttcagaagc 98760 acgtagtttc tatcgctgac gaacgtcagg attgcttggc atttatctct ccaccgaaag 98820 gtctgttggt taacgttccc ctgacccgcg cagtggataa cctgattgac tggcgtacag 98880 gtagtggtgc atttgatact gataacatga atatcagcac gacttatgca gcgattgatg 98940 gcaactataa atatcagtat gataaatata acgacgttaa ccgttgggtt cctctggcag 99000 ctgatatggc tggtctgtgt gctcgtactg atgatgtttc tcagccttgg atgtctccgg 99060 ccggctataa ccgtggtcag attctgaacg ttctgaaatt ggcaatcgaa ccacgtcaga 99120 ctcaacgtga ccgcatgtat caggaagcta tcaacccggt tgttggtttc gcaggtggcg 99180 atggttttgt tctgtacggt gataagactg caactaaagt tccatctccg atggaccata 99240 tcaacgttcg ccgtcttacg aatatgctga agaaaaatat cggtgacgca tccaaatata 99300 aactgtttga gcttaacgat aacttcactc gtgcatcatt ccgtatggaa acttctcaat 99360 acttggatgg tattcgtgcg ctcggtggca tttatgaagg tcgtgtagtt tgtgatacta 99420 ctaacaacac cccagcggtc atagaccgca atgagttcgt tgctagcatt tattataagc 99480 ctgcgcgcag catcaactat atacagctta atttcgtagc cactagcact ggtgctgatt 99540 ttgatgagtt gattgggcca caataattat catcgcttag aaagaattaa ttagcagcaa 99600 aaccgtagaa ccattataca gtgctataaa taaaagcata atcggttcta cgggctcctg 99660 tggttgattc tgggaagcca ttatcttgag atgatgaatc atattaccta gtataaatac 99720 atgcatcagg gacgcgttcg cgcgacctgt gtctataaag aggattcctt aatggaactt 99780 accgatatca ccagagcctt cgagtcaggg gactttgctc gccctaactt gttcgaagtc 99840 gaaatcccat tcctgggtaa aaactttgct ttccattgta aagcagcccc gatgccagct 99900 ggcattgtag aaaaagttcc ggtcggttac atgaaccgta aaatcaacgt agcgggtgac 99960 cgtacatttg atgactggac tattacgatt tataacgacg atgctcataa agttcgtcaa 100020 gcaattgttg attggcaaaa tctgtgtcac ggtatgacta acgaaatcac cggtgcagct 100080 cctgctgatt ataagaaaac tgctactgtc cgccagttcc atcgtgacgg caagaccatt 100140 acgaaagaag ttgttattac cggcatgtgg ccaaccaacg ttggggaagt ccagatggat 100200 tgggacagta ataatgagat tgagacgttt gaaacgacgt tcgcaattga ctggtgggaa 100260 taagtataaa taaagttatt atgaggcttc ggcctcattt cccaaacttc ggagactcta 100320 atggcaggtt ttaacacaat actaaatttt cttaagcctt gggctaaaga agatgaacaa 100380 gaatataagc aacaagaaaa aaataattta gagtcaatga ccgggccgaa gtttgatgat 100440 ggggctcgcg aaattgagtc acaagaacaa aacgtcccgt ataatgcatt gatgcaacag 100500 atgttcggga gcaatgaacc tgaagtaaaa aataccaggg aattaattga cacttaccgc 100560 aacctactaa ataattatga agttgataac gcggtgcaag aaattgtgtc tgatgctatc 100620 gtttttgaag acggtaaaga tgttgtagct cttaaccttg atgctaccga atttagtcag 100680 aaaattaaag accgaatcct tgaagaattc gcggaagtta ataacctcct taatttcgag 100740 cgtaaaggtt ctgaccattt taaacgttgg tacgtggatt ctcgaatttt cttccacaaa 100800 attatcaacc catcaaaact taaagatggt gtccaagaac ttcgtcgtct tgacccaaga 100860 caaatccagt tcatccgtga aatcgttaca cgcatggaaa atggtacaaa aattgtcgac 100920 ggttacagag aatattttgt atacgatacg ggccatgaaa gttattgtgc cgatggtcgt 100980 atatatgaag caggtactaa aataaaaatt cctcgtgcgg caattgtata tgcgcattct 101040 ggattactcg attgttgtgg caagaatatc atcggatact tgcaacgagc aattaaacct 101100 gcaaaccaat taaaactatt agaagatgct ttagtcattt atcgtataac tcgtgctcca 101160 gaccgccgag tcttttatat cgataccggg aatatgccgt ctcgtaaagc agcagcacac 101220 atgcaacata ttatgaacac gatgaaaaac cgtgtcgtat atgatgcaac cacgggtaaa 101280 atcaaaaacc aacagcacaa catgtcaatg acagaagatt attggttgca acgtcgtgat 101340 ggtaaagcag tcactgaagt tgatacactc ccgggtgcgc aaggcatgtc ggatatggac 101400 gatattcgct ggtttaagaa caacctatat caggctctcc gaattccatt atctcgtatt 101460 cctaacgacc agaacagcgg cgttatgttt gatgcgggtg cgacaattac tcgcgatgaa 101520 ttgaatttct ctaaatggat tcgacagcta cagaataaat ttgaagaagt tttcctcgac 101580 ccgcttaaaa ccaaccttat cctgaaaaaa attatttcag aagaggaatg ggaaaaggaa 101640 ctaaataata ttaaagttac gttcaaccgt gatagctatt tcaccgaaat gaaagatgca 101700 gaaatcatgg aacgcagaat caatatgcta acgatggctg aaccgtttat cggtaaatat 101760 atttcgcatc aaacggctat gaaggatttc ctccagatgt ctgatgaaga aattaatcaa 101820 gaagctaagc aaattgaatt ggagtccaaa gaggctcgtt tccaaaaccc tgaagaagaa 101880 gaagaggatt tctaatggat gatttatttg aagccattaa atcaaacgac ctcgtagccg 101940 ttcgcaaata ttttgctgaa aacattggcc cagtatgcgg tagtttgatt gaagaacgta 102000 agattgaaat tgcacgttcc atcatgattg aaggcgaaga gcctaaagaa gaagatgaag 102060 acgacggtaa agcagaaaag aaagacaaaa aagaatctga tgacgctgac gaaggcgacg 102120 acgatgaaga tgaggacgat gaataatgtt tattatccct gatgattacg aattaactct 102180 tgaaaacatt gaaagtgttt ccgaagaagc tcagggacaa tttgattcat tatctgaagc 102240 cttagaaaaa gacgatataa atattattgt agaaaacatg tttgcagccg gtgaatacga 102300 aaaagcaacg gttatcggcg ctctgaatga aggtgttcag cttaacgaat ttatcgttaa 102360 acatgtatcg tctcgcggtg agttaacccg cacaaaagac cgagctactc gtaagcgtaa 102420 tgctttccaa acaactggtc tttctaaagc taaacgtcgt caaattgccc gtctggctac 102480 taaaacgaaa agagcaaacc catctacgca aatcagagca gagcgcaagc gcaaaaaagc 102540 tcgagctaag cgtaaagcat tcggacttga ttaatggaac agaaaccaga gttactaatc 102600 gaacactggg gtcaaccggg cgaaattaaa gatggtgtac cgatgttgga atctcatgat 102660 ggttccagca gcggtttaaa gccaggactt tatattgaag gtattttcct acaagctgaa 102720 gttgttaacc gtaataaacg actttatcct aaacatattt tggaaaaagc cgttgcggac 102780 tatatcgccg aacaggttga aaccaagcag tctcttggag aattaaacca tccacctcgt 102840 gcaaacgttg accctatgca ggcggcaatt attatcgagg acatgtggtg gaaagggaat 102900 gatgtatatg gccgcgcgcg tattatcgaa ggcgaccatg gcccaggtga taaacttgct 102960 gcgaatataa gagcaggttg gattcctggt gtatctagtc gtggacttgg ttccttgact 103020 aaatcagcaa aaggttatag catcgttaat gaaggtttta aattgacggt tggagtagat 103080 gcagtatggg ggccaagtgc tccggacgct tgggtcaaac caaagcagat tacagaatca 103140 caaacggcag ttgccgatac cagtgccgat gacgccttta tggctctcgc agaggcttta 103200 aagacgttat aaataaatgt atatcttaac aacaggacta caaaatgctt aaagaacaac 103260 tcatttctga agctcagaat atcgatgctt ccgttgctct tgatagtatt ttcgaatcag 103320 ttaatttgtc cccggaagcg aaagctacct tcggcactgt attcgaagct accgtcaagc 103380 aacacgccat caaactggca gaatctcaca ttcacgccat cgctgaaaaa gctgaagaag 103440 aagtcgaaaa gaataaagaa gaagccgacg aaaaagcaga gaaaaaactg aaagaatctg 103500 cagctaaatt cctggaccac gttgctaaag aatggctcgc tgaaaataaa ctggctgtcg 103560 accgcggcat taaagctgac ctgttcgaat ccatgttcac cggcatgaaa gaactgttcg 103620 ttgagcacaa cgttgttgtt ccagaagaat ctgtcgatgt tgttgcagaa atgcaggaag 103680 aactggctga acagaaagaa gaaaccgcta atctgttcga agaagttgct aagcgtgatg 103740 cttatatcaa ttatgtccaa cgtgaagttg cgttgactga agcgactaaa gacctgactg 103800 aatcccaaaa agaaaaagtt ggttctctgg tagaaggcat ggaatactct gatgcattcg 103860 gtaccaaaat tggcgctatc gttgaaatgg ttaaaggtca ggtagaaccc accaacaaac 103920 caatcaccga agccgctata aataaaaatg tagacgatgc tgcggcactg aattacattt 103980 cagaagcagt tgaagaaaaa ggcgccaagc ctaccctgtc cttcgcggac ttgtctgctc 104040 tcgcagcatc acgaatttct taatttaata aggttataca acacatgaaa aagaatgcat 104100 tagttcaaaa atggtccgct ctgttggaaa acgaagctct tccggaaatc gttggtgctt 104160 ctaaacaagc tatcatcgct aaaatctttg aaaaccagga acaagatatt ctgaccgctc 104220 ctgaataccg tgacgaaaaa atctccgaag catttggttc tttcctgacc gaagctgaaa 104280 tcggtggtga ccatggttat gatgctacca atatcgctgc tggccagacc tctggtgcag 104340 taactcagat tgggccggca gttatgggta tggttcgtcg tgctatcccg catctgattg 104400 cgtttgatat ttgtggcgta cagcctctga acaacccgac tggccaggta tttgcactgc 104460 gtgcagtata cggtaaagac cctgtcgctg ctggtgctaa agaagctttc catccgatgt 104520 atgctccgaa cgctatgttc tctggtcagg gcgctgctca gacttttgaa gcactggctg 104580 caagcaaagt tctggaagtt ggtaaaattt attctcactt cttcgaagca accggttctg 104640 cacacttcca ggccgttgaa gctgtaaccg ttgatgccgc tgctactgat gctgctaaac 104700 tggatgccgc cgtaaccgct ctgttggaag ctggtaagct ggctgaactg gctgaaggta 104760 tggctacctc catcgccgaa ctgcaagaag gttttaacgg ttcccaggat aacccgtgga 104820 acgaaatggg cttccgtatc gacaaacaag ttatcgaagc taaatctcgt cagctgaaag 104880 caagctactc catcgaactg gcacaggacc tccgcgctgt tcacggtatg gatgctgatg 104940 ccgaattgag tggtattctg gctaccgaaa ttatgctaga aatcaaccgc gaagttatcg 105000 attggattaa ctactccgca caggttggta aatctggtat gaccaacacc gttggcgcta 105060 aagctggtgt gtttgacttc caggacccga ttgatatccg tggtgctcgt tgggctggtg 105120 aaagcttcaa agctttactg ttccagattg ataaagaagc cgctgaaatc gctcgtcaga 105180 ccggtcgtgg tgcaggtaac ttcatcatcg cttcccgtaa cgtagttaac gtgctggctg 105240 ctgttgatac ttctgttagt tatgctgctc agggtctggg taaaggtttc aacgttgaca 105300 ccactaaagc tgtgttcgcc ggtgttctgg gtggcaaata ccgtgtctat atcgaccagt 105360 atgctcgtgc agactatttc accatcggtt acaaaggcgc taacgaaatg gatgctggta 105420 tctactacgc accatatgtt gcactgaccc cgctgcgtgg ctctgacccg aaaaacttcc 105480 agccggttat gggcttcaaa acccgttacg gtatcggtat caacccgttt gctgaccctg 105540 ctgctcaggc tccgaccaag cgcattcaga acggcatgcc ggacatcgtt aacagccttg 105600 gcttgaacgg ttacttccgc cgcgtatatg ttaaaggaat ttaatcctag attaagttaa 105660 ataaaaaggg acccttgagg tccctttctt gttttcatac cataccattt aaatgaactt 105720 ctactcggcg cttgataagg tctctgaagt taacattgtt ttcttgaagt ttcaaccagt 105780 taccattttt atctttagtg tgagtagaat aattaccatt ccaccagaaa tcagtgtcat 105840 atgctaagtt ttcatatttt ttaactagtt tgttcagagc attaccatta acaccatttg 105900 ggtatttgtt aaccaaggca ataacttcaa tttccagatt tttaaaggtg ttcagaagga 105960 aagttgcttt gttcattttt aaactccagg ttttgtttgt taactcagta tggaatcatt 106020 atgtcttatc ttaatctggt tgtaaacaac ttttataaat aaagtatatc acaaaaggaa 106080 atagcaatgg ctaaaattaa cgaactctta aaagagtcga ccacgaccac aagttcttcg 106140 attggtcgac cgaatctagt agctttaacc agagctacga ctaaactgat ttacaccgat 106200 ttagtagcac aacagcgcac atcccagcca ctggcggcat tatacggtct taaatacctg 106260 actcagcaag atgaattatc tttccagact ggtgcaacct attccggtgc agtatcagta 106320 aaagaccgtg cgaccattcc ggtttttgtt gctggcgcag tttatgctaa agacgatttg 106380 ttccagtttg aaaatgtagt ttataaagcg ctggtagcaa ctccatttgc tggagcaacg 106440 ggcgatgagt atgaccagct tcaagaagcg atggttaaat tgaccatccg tattatgtcc 106500 gaagctgcaa tcaccgaacg ttttgaagga ccacaggaag tcgaaattgc cgaagctaaa 106560 tttttggtta acaaatggaa tgctccggtt aaatctcgta agctgaaaag cactgttaca 106620 gtggaactgg cccaggacct ggaagcaaac ggttttgacg ctccaaactt cctggaagac 106680 ctgttagcta ccgaaatggc tgatgaaatt aacaaagata tcctgcaatc cctggtaaca 106740 gtttccaagc gttataaagt tgctgggcta tgtgatgatg gcctgattga cctgtcttat 106800 actaactctc cggaagcttc acgcaagttg tacgaaatca tgtgtgagat ggtctcccat 106860 attcagcggg caacttctta caccgcaaca tacgttgttg cttctacgcg tgtagctgca 106920 gttttggctg gttccggttg gttgaagcat cgtccacaag atgagaaata tctctccgat 106980 aatgcgtatg gtttcttgga taatggtcta ccgctttatt gcgatacgaa cactccattt 107040 gattatgtga ctgttggtgt taaagaagat ttcggtggga aagagatggt gggttcactg 107100 ttttatgcac cttataccga aggtcttgac ctggaagacc ctgaacatgt tggttcgttc 107160 aaagttgtcg ttgacccaga aagtttgcag ccgtctattg cattaatggt tcgttatgcg 107220 ttagcagcta acccttatac agtgactgct gatgataaac aagctcgtat tattgatgca 107280 acaaatatgg atttgatggc aggacgttca gatatgtctg tactgcttgg tgtgaaactt 107340 cctaaagttc tgactgaaat aaattaaaaa gggaccttcg ggtcccttta tttttattca 107400 ctaactaatg caatccaaac atgtcttaat gtttctctta catttgcgat gagttgcttt 107460 ttaacttcaa caggattttc cgtcagagac aattcaagac cttcgcgccc cgcttcttcc 107520 agaatatctt ggactgttaa ccccattacc tttccaaaat ccttcggtgt tacttcccca 107580 attttactga taacattgtt gatacggtta acagtcacat aatcagtgaa attccacagt 107640 aaatccatat cattttggct cagaaccact gcagccttaa taggcttatc agactttttc 107700 ttttcactga atttagagtt cttacattta atcgccacac gctcgccgtt aggtaaccag 107760 gcaggaacat caggttttaa tacaaatcct tcagcagtga atactttacc ttccaccttt 107820 gcatcaaaat cagtggtatt tgctgtagtc aatcctgcgt tatccactgt aaaattgtaa 107880 tcagggacca cagaatccag gtcattaggt agcttaatta attcttcaaa actacctgta 107940 gccaggcgcg gggcaacttt aaacttatga ataatacaaa aggcttccat aagtgtgtca 108000 gtcaggacgg attcagagcc atcttcctta gtaacgcgga tatcaaacac atagaaatct 108060 ttatcgccgt aattcacatt cttctgaata cctgtaccag caaactcacc ataaatctga 108120 tatgcctggt aattaatatc ctgcaaaagt ttctgcacag atttaataga atctacgtag 108180 ttctttaata caatttcata accatagaaa tcttctgcag gtaggatagg tccagtgcgt 108240 ttcgcgcatg tcacattgtc ttgttcaata attaatgaaa aattggtacc atgaattttc 108300 tcgcgggcta cccattcacc gctggtgaag ccttgtaggc gaattttttc aatgaatttg 108360 ttgttatagt gattttcaag gctgttatat ttcttaaaca tattctctca cttttgatgt 108420 tataaatatc attataaatt agacccacta tactttcttt ctctgaaagg aattaatagt 108480 tgcaaagggt ctagaaccat tataccgcat ttttatttga agcaaaaagg gtcagaattt 108540 tacatacagc attggcttgg attcttgcca atcataattc caggtaaaag atgtaaattt 108600 ttctgctcgc agaaattcag atatctttgt gcggtttcct tcttcatact ctttttcaga 108660 aactgccata ttaacagcat ctttgccatt ttcagcggcg gcaatagcca ctttgcgaaa 108720 tatagaaata aagtcatttg caactttatc ttgtgactct ttggctattt ggcgtagttc 108780 ttctgcgaat gtcatttctt cacctttttg ttattcaaac gtttacgata aacaatcttt 108840 tctttcgctt taaatttagc tttagcttgc tctacatcaa cccaggcctt tgccatctct 108900 ttagcagtcc cgcctaatac cttggcgatt tcaataaatg tcataccaga ttcatgcaga 108960 aaatgtactt taaccttatc cataatgatt ctcatgttga gttagtgaag ctatcttaac 109020 atggaatttt taacttgtaa acagggtttt tatgccaggc aacgcttgcc cagtaccata 109080 aaaaataaag ggaccttcgg gtcccttttt aattaataaa cgtaatcgta tataatacca 109140 gcttctaaag cggtgcgatg aataattcga ccattacgag attcttgtac atcaacctct 109200 ggatagtcgg taatcatttt ttgaagggta tatcggtgca aataatatgg gctatctgga 109260 gcatcagttt tccagtcttg tccagcaaga gtcattttct gaatttcgtc cataacccac 109320 caaccacacc agatataagc tgaattacga actggcagcg gatgaacgta aggtaattcg 109380 ccatctggaa ctggtgcgac tttagccgtg actgttacat taccagtttt tgtgacagtc 109440 accgggtcat aatcagtcgc cgaaaccaca acagaaactt caataacctg tgaaccttca 109500 gcgcttgtat cgattaccag agtatccgtg gtgccaacta caggagaacc gtctttcttc 109560 caagaatagg caaaagtagc atcaccaggt gcgccaacta catccgcttt aaaagaagcc 109620 ggagtacctt cctgaacatt aattgattct ggggttaatg tcacagaaac atcagacata 109680 gttttattct ggatagttaa agtcgtctca gcttcagctg tttctggttc gccgtccgct 109740 ggtgtagttg ttgcaacaac tttaatagtc ttgctacctg ctggaccaac agcaacataa 109800 tccattgcag cagtcacaga agattgagga acaccatcaa cggtccagac aaatgattcg 109860 gtaccttcag ctgcagcacc ggaaccggtt gcagtgaaat tggttgttgc tccaataacg 109920 gccgtagccg ccaaaggagc aatatctacg gtataagcca taataaaccc ttattttaaa 109980 gtgacaaaag atgagttacg agtttctcgt actagaacag acccattacg attgatgtaa 110040 taaatcaaac tgaatagagt ttggtgcgcg gtgggatgtg ggaaagcagt tggacgttct 110100 ttccaatctg gagtctcggc aatccactga taaatccacc atggaacagt acagaatcca 110160 gggttcttac caataagcat taaattcggg ctaaagtttt ttggcaaatc aaatacaggt 110220 ttttcagatt cgataacctt agcaacagca gcttcgaact tttcttcgac aaaaggaacg 110280 tcctcttcga ccaaaacaac tggttcttcg atttcaacaa cttcaacctt tgcttcggtt 110340 ggagcatcaa ataagagctt agcttcttct ttagctgact catcgccatc aaatttagca 110400 cctgacaatt cgtcatcggc atcgataagg tcagtgatgc tcagtccctc attatcatca 110460 ggcatgggca tgtcagcaaa tgctttaagc cctgcttcca ggtcagccaa catattatca 110520 aaggtttttg ttttcttaac atcaacacca agtgatgctg catattcggc taatttttct 110580 ttggcttctt ttttctcgag gacacgaatt tcctcaatgt agttcttgtc aatcatgatg 110640 aacctcggtt agtgtataaa tatagatata tttattacct tggaatcatc atggatatta 110700 aaatcaactt tctaaatttt agtcacgtgc atgttgaatg tgaagatgga atcttctacg 110760 aactccgtga ttacttcagc ttcgaagctg aaaactatcg cttcaatcca aagttcaagc 110820 agggagtttg ggacggacga atccgcctcc tggattacaa tcgtaaatta ccatacggtt 110880 tggttaatca aattaaaaag ttcgctgacc agtttggtta ttcattctgg attgacccgc 110940 aaatcaccga gaaagaagaa atctctcgcg aagaattcga caaatgggtt gagtcaaaag 111000 aaatctattc aggcaatacc aagataacgc ctcattggta tcaatctgac gccgtatatg 111060 acggtctggt caacagaaga aagattctga acttacctac gtctgcaggt aaaagcctca 111120 tccaggcgct tctggcgcga tattatctcg agaactatga aggcaaggtg cttatccttg 111180 ttcctacaac cgcgcttgtt gaccagatga ttaatgactt tgttgattat cggcttttcc 111240 ctcgtgaagc ttgtctgggt attcgttccg ggactaaacg cgattctgat gcgatgattt 111300 atgtatcgac ttatcagacg gctgtaaaac aacctaaaga atggtttggc cagtttggcc 111360 agtttatgaa tgacgaatgc cacatggcta caggtaaatc gatttcgacg gttatcgaag 111420 gccttacgaa ttgcatgttt aaatttggtc tgtcaggttc actccgtgat ggcaaagcca 111480 accttatgca atatatgggt ctgtttggtg atatctttaa accagtatca acatccaaat 111540 tgatggaaga tggccaggtt actgacctta aaataaatag tattttcctt cgttaccctg 111600 atgaattcac tgttaaaatg aaaggtaaaa cttatcagga tgaaattaag gtaattacaa 111660 aagctacacg ccgaaataaa tgggtaacaa atcttgctct caagcttgct aaaaaagatg 111720 aaaacgtctt tttgatgttt aagaatatcg agcacggtaa atcgatgttt gaaatgctta 111780 aggaagctgg tcatgagaaa gtgtattatg tttccgggga agttgatacc gaaacccgta 111840 atgctctgaa ggtcatggca gagaatggaa agggaattat tgttgtagct tcatacggtg 111900 tattctcaac ggggatttcc gttaaaaacc tccatcatat tattttcgct cacccggtca 111960 agtcaaaggt aattgtactc cagacaattg gtcgtgtgct tcgaaaacat gaatccaaat 112020 cacaagcaaa cgtttttgat atcgttgatg atatgggtgt taaacctaaa tcagcaaatg 112080 ctaaaaagaa atatgttcat cttaactttg ccctaaaaca tggccttgaa cgtatacaac 112140 gatatgctga tgaacgtttt aactatgtaa ttaaacaggt ggaaatttga attatcaaaa 112200 aatatatgat gatttaattg ggagtcgaaa ggcttccaaa ccgattggat acacggaatc 112260 tcaccatatc attccagaat gtatgggtgg acctgataca caagaaaaca gagtcaacct 112320 tactccggaa gaacatttcg ttgcgcataa attattggcc aaaattcatc ctaatcatta 112380 cggtcttata aaggccgtca ttgctatggg tatgagctct cgaacacatc aacgacaatt 112440 caataaatcg tatggatggg agcgtagaca aatgtcagtt cttcaaactg gtgaaggcaa 112500 tcctttttat ggtaaacacc atactgaaga acataaagaa tatatgagcc aattattaaa 112560 gggtcgcaaa ataacttggg ctgataaaat atccgcgaca aagaaagcga atccgtataa 112620 atactctcaa gcacaatgtc aagctatatc tgaaagaaat aaaggtgaag gtaattggat 112680 gtatggtaaa tcacatacac cagaagcatc aaagaaaatt tcagataaag caaaagaacg 112740 ttataaggac ccatccaaaa atcctgccgc taagaaagtg gttttaaacg ggattgaata 112800 tggttcgatg aagcaagctt ctgtagcaac ggggctttct ttatataaaa tcagaaattt 112860 aattaaacag gtggaaatat aatgaaatcc ttcgaagaaa ttatgtacga agcatcaatt 112920 gaaggcttta tggctaagat tggctcctgt cagaccatgg acggtctgaa ggagttggaa 112980 ggctattata agaaacgcag caaagaagct gaactgaaag attccgatga tatcagtgtt 113040 cgtgacgctc ttgccggtaa acgcgccgag cttgaggata tggacgacga cgaagacgaa 113100 gatttctaaa taaaaaggcc ccaaccaaaa ggaaggggca aaaccaaaaa atggttaaca 113160 acactagcta aattaaaagt tgggtctcgt tcagttgctc ttctgtattt tccgtaactg 113220 gcaaactctg aacataatta taacaggcgc ccggatgcac cggaccttgg tctgtatcca 113280 caaccaatgc agaatcgatt ggagttttac agacaacaca aatcttatct gacatgattg 113340 tctcctcagt taaacttaca tatctattta gtgctcttca taatagcagc acgagctttc 113400 atctcattaa caatttcagt tattcggttt gcagagataa ccatctcgtc aagaatctta 113460 ctaatatttt tcatttaatt ttccttaaag tatctcatac cggtatctat ccccatattg 113520 tgagaataga acgcatcatc gatgtatcct gcaaatttac ctttaacata gataacatct 113580 tcaccacaag ggtggtcagc gcagatacga atatccgcac gattcaattt ccattccttc 113640 atcatatact taatttcttc ttcgaactct tcgtcttggt taaacgcatc gtcgatagaa 113700 tatcgcatta cttgcctgcc tcgaattgtc ttatttctaa catatgttta agtgcgaaac 113760 ttcgtgactt aacaccatct aatgcagcgc tacagaagtc ctggagtagt gcccaatact 113820 gaattgaagt atcaatcttc agtatgtttg aatctgcggc catgactgtt ttcaattctg 113880 acttctcgta tacatccatg catacttctt cccctgggtc accccttcct gtataatggt 113940 ctagccgttt tttcagtgca gttttcttct gaatctcaag acgcattatt tctttcttgc 114000 agttggtata caatctcaac catttgctat gcaacaaaac gttgttttgt gtttcatatt 114060 gaagttgcgt accatcaatt tttaaatctg catccaactc atcctgaaag acttctaact 114120 tatattcaat ctctgttttc ataatgtatc caatgtaagg gtctagaacc attatacagc 114180 cataaaatta aagcattaac cgttttgact taactgaatc ctgatttgtt cgagggtgtc 114240 agggtcatca acaatgctaa actgaaccgt tacgataatg gagttatcat catacatagg 114300 gacaactgca acccgtagat tgcttatccg tggttcgaag ttccgcacag cggagacgat 114360 gttgcgttta atggtatctg tgattaatgg agtgatattc tcaaaaagct gattacttat 114420 atcacatcca aattcaggca taaaaggcct tgaccctttt cttgtggtga ttattcccag 114480 aaggctgttc ttaacagacc ttgcgccggt tactttagca acgtctttat cccaactcat 114540 tgacaagtca ggagacacat cgctatacat tttgtttata ttcatttaca taaacttaaa 114600 gaattcttta agccccttaa ttacatggac agaatgcgct ccacactcgc aagagatagg 114660 aatagccatt tggacctgtg gcttgagaag ttcttccgtt agtgcattta tatcatcgag 114720 ggtaattgca ttgtacaggt cctcgatttc ggcttcattt aagtcatcaa caacgatgcg 114780 ctcaccgcct tccgtgataa tatattcgat acaacaagca atcatttttc ctttatcgcc 114840 atcttcaaac agcccaggat aacggaattt aattttaaac gccccgaaat cacgaataac 114900 ttcagactca tccacgatgc ttgcgcgact tatagtcaca ggaatatctt gttcctttcc 114960 gcactcacat atccatgtat gctcgacgtt aacagctccg agactatttg cccagagatt 115020 aataagaagc agttcagctt cctgtttgtt taacccttta gcatcagtgc atttattaag 115080 cagttcaatt attgcaggtt ccatacgctt ttctaatttt gcttgaagca aatctttgta 115140 ttcgcgcaag gtaaaagccc tacaatttat agttttattt ttaatttgga caataaattc 115200 ataagtcata atttgctcct ttactgtatt tataaatatc aataaagata aaaggggatt 115260 ctatggccaa tatcgtaaga tgcgaaatgc cagacggagt tcatcgtttc aaaccgttta 115320 cagtcgctga ttaccgtgat ttactgcttg tgcgaaacga catgaacaac aaacccgtcg 115380 aagaacaaaa acaattatta gacgaactgt tggaagatta ttttggagag tatccgaaag 115440 catggaggcc atacatattc ttgaaggtat tcacttcctc tatcggtaag acaaaagtcc 115500 cggtagcgtt cgaatgtcaa aaatgtaaaa agacaaagca gactttgttt aatatgagtc 115560 aagggccatt gataaatcca acaatggaaa ctgcggggat aaaaatacaa tttaagttcc 115620 ccgaagtgga atataaagat aagtcggagc ttgttctaaa caccatccag actgtcgaag 115680 actcagaaca aaagtacaat tgggaagacc ttcctgaaga aacgaagatg caggttattg 115740 atgcaattga tatagagagt ctcgaagagc tccttaagaa aatgcattca gtttatattc 115800 catttacata tggttgctgc gaaaaacaca aaaccgaata tacagacttg attgatgtct 115860 ttaagcttat ccttaacccg gatgaagtct ttgtatttta tcaaattaac cacttgctgg 115920 taaagaacca atatacgctc gatagcatca tgcacatgat tcccatcgaa cgaggtattg 115980 ctttatcgtt agttgagaag gacttgaaga aatgacagct aaatctatgc agcgtgaagg 116040 ctttcctaat atcagcatac gcctttacga agattatgat gcctggctcg aacaccgttt 116100 tgtagaactg gctgcaacat ttacaaccat gaccatgcgc gattctcttt acggaagaaa 116160 cgaaggcctg ttgcaattct acgacacaaa gaacttgcat acaaaaatga acggtgaaca 116220 aattatacaa gtctccgtta agaatgccaa ctcagaccga acccaatcca ggatatacgg 116280 aagtaagcac tttgctgttt ccgtggattc aaagggtgat aacataatta caattcagct 116340 ggcaccgatt catgaaattg aagacctgaa atttagtcgt atgttttatc cgagtgtgca 116400 agaaacattg gtcgaaatga ccggggtaat ttatcaggat agacctttgc ttgctcctcc 116460 gataaacggt ataaacgttt atgctcctaa tgttccgtgg tgtgattcga tgagccagta 116520 tatggaattc atcagggaag ttggtcttgc agttgaatct gataaatttg tatttgtatg 116580 gcaagatatt gacggcataa gtatccagga ttatgaatgg ttaatcgcgc aagagccgat 116640 taatttcgtg gtgggggaac cccgattggt tggtcagttt gtacaaaacc tcgagacccc 116700 tttggccttc gatttcgaat ggttaacaaa ggcgaaccaa catacaagaa aaccatacga 116760 aaacgcaacc gtgtatgcgc attcattcct tgataagaac gcaaccagaa taacttttgg 116820 ggaaggacaa aacagcattc tggtatctcg ttctggcggc tattcggatt atacatatcg 116880 aaatggtttc gaggaagcac aacgattaat aaccatgtca caatatgacg gttacgcgca 116940 ttgcaaaaca tacgggaact ttgaattaac tccgggcgat aagataaatt tctatgaccc 117000 gaagaaccaa ttcaaatatg atttttacgt agacgaagtt attcatgaag tgagtaacaa 117060 tacatcaatt acaaacctct atatgtttac caatggtaag cctatcgaag ttgaagaacc 117120 accgaaggtt aaaaatgaac ttaaaactga ttctgccgac gaagaaaata cagctgggtg 117180 ataaagagat tcacgttccc aagcttggac tcaagcatca caatctgatt aaaaacgaac 117240 ctaatcctta tgttgcatta agaaagatta tgcaatcgat attccctaat cttacagcag 117300 ccgaatccga ctttgtatct ttacatcttc tggaattcaa tggtaagcta aaagacaaac 117360 gcgagattaa tggatttaca tataagctgg atgatgttta tatctgtcag cgccttgaat 117420 tctcttatca aggaaagaca tttaaattcc gctccaataa accgtatgaa gaattcggac 117480 cagttgacaa tctcctggag accctttatc tcggggaaga tgttccagac tttctggata 117540 tgcctgcttt tgttgctaag tgggcagacg atatcacaag cacattagca atcccgggcc 117600 cgaatggtcc tgttaagggg ttactgaaaa taatggaact cctaaatgaa taagaactcc 117660 gaacaaacga gcttcagacg cggtggacct aataaaaagc ttatcgaaga attagcacca 117720 caaaggaggg cggaagccct ctctgctgaa cagaatgatg aattatcgaa tctgaataca 117780 actcttacaa atactcaggc tgcaaccgaa cttgtatcgg aagctattga ggataagggt 117840 aatcagatta tagagaatat ccaaaccaat aatggagtcc tccaggatat cagtgctggg 117900 gtcgaactga cggccgaagc caccgagaag actcaacaag ggattaaaaa cctcacggat 117960 attctctcgg ataagctcga caaactcagc gcaatgataa gtggtaaact aggcgtcacg 118020 tctcctgtgg ctggttctga aagccttaaa cctgtggagg atgcactacc agagcctgaa 118080 gagaataagc ccacggcgag cgttccaacg ctcataccgc ctgaagaaca aaagcccgac 118140 gctgacttca ttccggaacc agaacagcca aaaaccgatg cagaaggcaa agagactaat 118200 acctggtcac ttggtgataa gcttgatacc ctttcgaaga taaccgaaaa aggatttaag 118260 gcatcgattt ctgtagccga ccgaatctcc ggaatgttgt tcaaatatac aattacagct 118320 gcagccgaag cagccaagct tattggtgga ttattgttgt tggtattcgg tatcgatgca 118380 atacgagttt acttccaata tttcatgaag caattcgaaa aaggatgggc cgaatttaat 118440 gataagttca aagaatgggg cccactgctt gaaggactaa tgacttgggc taagaacgcg 118500 caagcgatgt ttagtgaaaa gaattggttg ggattagctg aagctattat ccgtgggatg 118560 gtcaacttaa cgaagaacat ggctcaactt cttatgcttg gtatttcgaa actgatttca 118620 gctatcttaa gcaaaatccc tgggatgggc gatttagcag ataatgtgga agcctcagct 118680 ttgatgtctt atcagcaaaa tacaggggca actctggatg atgaagacca aaccaagata 118740 gcaaaatatc atgacaaacg ttcggctgaa gcaatggaag ctacagagaa gatgaataag 118800 aagtataagg ataaacctga actaattaat caagccgaaa aatatggtaa ccttaccaag 118860 gaacaagccg accagcttcg cgcgggtggt attgacacaa gcttcagaga ccttccggaa 118920 gaagaacgtc tggactattt caagaagcgt gacaaagcac aggctgatat tattcgtttg 118980 acccaaactg ctgacaacct aatgaagcca gacgcaaccg ataaaaagaa tgcgatggaa 119040 atgagagcta acattgaaaa acaattagct gacccatcta tggcaaaagg tggagctccg 119100 aaagacctga atatgcgcgc cctgttggaa aaacttgata agtctctcga gaagttcaaa 119160 gacgaaccga aagttaaacc gccggatgtt aagacttctc cggatgcaca acaagctgcg 119220 aaggttgacg aaggcatgaa ggccaaagaa aataaataca aggatgcacc agctcaggca 119280 caaattaaca cagtcaataa tattcagaag acttccagga cgcaatacaa tatgcctcct 119340 caaagttcaa cccctgcccc tggcatgaga caggccacga gaattaacta aggaataaca 119400 tgaaagtaaa agaactcgat attgcgggtt tatttaacgg aggttctaag acctccgctg 119460 ggcagtcaaa agctgcccag actcaagcta ctattgtggc gcaatatcca gcagaacgag 119520 cttccggtaa tgattcttca gatgatatgc gagttaatga cctttataaa aatggtctgt 119580 tattcacggc atacaatttc agttcaagaa catctccgga attacgctca gacaggtcaa 119640 gccaattgac ttcattaaag aaagtttcca atggcgcttc atttaacccg gttaaaagtt 119700 taacatcttt tgctaaatcg aaattaaccg gcgctggctc aacaggtaaa agttttgatt 119760 caaacgctgt tgcaaatatc ctgttgcctc gttcgaagtc tgatgtagaa tccgtatcgc 119820 accgattcaa cgatgttggt gaatctctta ttacgaaagg gggcggctca gctacaggta 119880 ttcttagtaa catcgcatcg actgcagttt tcggcgcact cgaatcagtt acaaatggtg 119940 taatggccga ccatggtgaa cagatttaca caaccgcgcg aagcatgtat gctggtcctg 120000 ataacaggac aaaagtttac acatgggaaa tgactccacg ttcggcccag gacttaatcc 120060 agatagtgaa gatatacgag atttttaact attattccta tggagaaacc ggcaaaagtt 120120 cgtttgcttc cgagctgaaa agaaaaaatc gatacatggt ataaatccac cttcaaaaaa 120180 gaagcaatcg ataactttga cggcaagttg ctcggtgaag agataacaag cttcctaacg 120240 aacgttctgg tggtctctaa ccctactatc tggtatatcc gcaacttcgg cgatacaagc 120300 tcgtacgatg gtcgtgggga gctatttggg ccgtgtcaga ttcagagcat acgatttgac 120360 aaatcaccgg atggtcattt cggtggatta gctattgctc ctaacctacc atcgactttt 120420 gtgcttgaaa tcactttccg tgaaatcatt acgcttaacc gtggctcgtt atatgcggag 120480 ggtttctaat gtttacttta caagaattcc aaacgcaggc gattaatatc gacttacaaa 120540 ggaataacct ttttagtgtg gtcttcgcga ccgcaccttc ctctaaatct cagaatcttc 120600 tggaccagtt tggtggcgca ttattcagca accttcctgt taactcagat tggttcggat 120660 taacacaagg tgacttaact caagggataa caactcttgt tactgccgga acacaaaagc 120720 ttattcgcaa atcagggata agcaaatatc ttattggggc aatgagtaac cgtgttgtgc 120780 aatcattatt aggggaattt gaggttggta cttacctgct tgatttcttt aatatggctt 120840 atccaacggc tggtctatta gttcactctg ttaagcttcc ggataacaca ttgaactacg 120900 aaatggacct taaccataat gcgccaaata tcaagataac agggagagaa tattctccgt 120960 tggtattgag ctttagaatg gattcagagg cggggaactt cagagcattt aacgattggg 121020 ttaactctgt acaggaccca gttacccagc taagggcttt gcctgaagac gttgaagctg 121080 atattcaggt taaccttcat tcgcgtaatg ggttaccaca tacggttgtt atgttaaccg 121140 gttgtgttcc tgtaagtgta tcagcgcctg aattgagcta tgaaggggat aatcagatag 121200 ctacatttga tgtaactttt gcttatcgcg ttatgtcaac tggtgcggtg ggaagagctg 121260 cagcattgga atggttggaa gataaggtta ttaaaggtgt atctggtatt agttcagata 121320 acaacttgaa cgcagaagtt gccaaattga gtcgcttgtc tggtgctcaa tctggattaa 121380 caagtctata caatacgttt actggttctg gcggaagtca acgtatatta taaaagaaag 121440 gaccgaaggt ccctttttat ttgcggaatg aaatgaaacc tgctgcagtc attacaactt 121500 ctttcttagc aaagactgta attgcaattg gtgcttcaga ttcaagttgc ccagttacta 121560 caccattaaa aatattttct gtttgttcag aatttacaaa gttttcaaca gggcgaaggt 121620 caaacttctt atcttcgcca aacacgcgcc gcagttcatt acccaccgcg ctgtcaaact 121680 cttctgaagc cgggataaca ttctcaacta ccagttcctg gccattaaag cgaaaagatg 121740 atttcatttt gttttcctca tgtgcaattg atatgaacat tgtatcgcac ttccttgtgc 121800 ttgtaaaccc cctacgaaag gaatttagga ggaatagctt ccaaattaat acaactagca 121860 agaataccaa gaccttgatt gactttcttc gttaccccag aggatgcaaa cgatgcaaag 121920 tcaacagatt ctactggctc ttctgtttcc tcgccctgat aaacgacaag ctcaccagtt 121980 tccatcaaca catcaccatc atagataact gcattttcat ccagttggtc agtggtctgg 122040 atatcagctt ccagcatttt gacgttcgcg tttcctttat acgaagcatc cgtaattttg 122100 gtaatcttaa ctacagttcc acgtggaaga ataacttcct gctcggatgg gttgcctgac 122160 aattccccag gaacaattac gttaattaag tctgccccag aaattttcca accgacatga 122220 atcattgcgt tgtccgggtt tgcacctggg atatttggta aaacaatttc gtcatcagcc 122280 tggaaagatg tcccgtcgcg atagtcctga tgaactgcgg ccatagcaac gttcgccacc 122340 cagccaccaa agataattgg tttaaatgat gttgatacat agttcctaaa atagaataat 122400 ttcgactgtt taattttatt aaatgtagga acgtttaaat attgaccacg ccaaagagtt 122460 gtattaggtg gcaacttatg tccaatttca aaggccgaat ccaattcctt aattgtttcg 122520 acaactctgt ctagatttag gaattcggtg tcttcttcat aataattacc catcaaataa 122580 ttattaatat cgacatagtt ggctccacaa taatctgcaa ttgcttcacg ctggccataa 122640 tggaaacggt tgtcagtata ccctgctgtg aaattaagag tattttctga tgcttcttct 122700 gcaaagtctt taactgcaac acgaatggcg cttgtgtatt cttcttccca gagcgtggct 122760 gcataagtgg ctgcatccag gttaggagga gcatcattca tttttctttc gataaatgcg 122820 gctttgcgaa ggttaagaat ctctgcacat gattcggcaa aaatctgcat ctgcttcata 122880 ctaccaactg gagcacgttt aagttcgcga tgcatacggt taaaaattgg gtcagagtct 122940 tcaacacgaa tttcttcttt ccaaacacca tttgcacgca tagccgtatc tgctgcgcgc 123000 atacgagcca tagtattcgt ttcggaaggt cgaacaaaaa tttccagttc ttcgtaatca 123060 tcgataagtt ctgccgtagc cggtcgacta aacttagcag cttctgcatc aatttcgtcc 123120 caaccttctg gcacggaatc acttggagcg tactgggctt gtgcaatagc tcggcgactg 123180 attttagttt tctgggcaac agcagcatcg gtctttttgt ttgctgcatc aatcagaact 123240 tcggctgcga tttcttgttt ggagacttgt gcgcctgtat ctttattagt gaatacatcg 123300 ccgactttag tttcaacttt ttgatatttc tcagggaccg gtggcatacc atcaaccgaa 123360 agcaggtcaa cacccttttt gtacataatt acataagcgt tctttttacc gaggccggaa 123420 gcattcggaa gaacatgata acgcccgctg gttttcattt gaatcagtcg attcataata 123480 cgaataacag cttggtcttg ccctttcatc tttttggatg ggaaacggaa caagactaca 123540 tcaggtttgc ctagtttaat gctttcccag actgtaacaa aaatcaggtt aatcgcatca 123600 agaggttttt cacctagacc acccttcaga ggaacagcat ggccatgtga agtcccggat 123660 aacaggaata cctcggcaat tgcatcgcca cgtttatatt gtttaactgt ttctttgccc 123720 gtgaattgca tcaaacgggc cataagagaa ttggtcccag gaacaccgaa ttgccaaacc 123780 tgcgggacct tgtattttgg attcatatta atgacaggat aaccgaaggc ttcgatatat 123840 ttgtcaaaga gttcatttaa ttgttcagac atttttaacc cttaattgag aaggtgtaca 123900 agtttttcca tagacaaatc ataagagatt ctgtcatatt ttttcaaaag cgaatttgat 123960 tcgtctatct ggtcattttc taagatagag cattcaacga gaatgtattt acccatcgaa 124020 cccatcatgc cagctgtagc accatagact ttatcaatct taagcattgt tccacgtgga 124080 agaataactt cggtttcaac ggaccagttt gccaggtcac caggaataac taccttgata 124140 ttttctgcac ctttgataat cataccaatc ttgattgtag gtttcgccga accagttgca 124200 cttttaatag ttgcatcgat tgccccatca tcgatatcac tcgataatgc atttgggtca 124260 aggttcgcag cggtgtggcc catacgaccg aaaatatttg gcgccagaga tgtactcgca 124320 aacataggaa gatacatcgt tttatttttt atggaattaa ttgccatatc ggttggcatg 124380 acttgagcac ggtagatgat agttcctttt ggaagcttaa caccgttttc aaatacttta 124440 tccatatcgg aaatcatttt gatttcgctt tcttttgcgt cagctttccc tagcaggaaa 124500 ttattgattg cagtatattt tccgcgagta tacgccttaa ttgcttcggc ctggtcttca 124560 gttatttcag ggttgtcgga gatatgaaat aatggttgga tggccttggt gtaaatttca 124620 tctgcgataa tacgaatagc ttctttagtc ttatcttcgc gcgcccaggt ccccacaatg 124680 gagtcgtcaa gcaacatatc agcaactcta cgcaatgtat caacaggatt ctttgaggcc 124740 cgcaggacgt tcgaaaactc acttattttg tgttccatgt caacttcttt aagagcatta 124800 aacacatcca tggctacacg agtttttgtc ttatcttcac caacaccacc ttgtgcaact 124860 aaatcaaaat agtctgcata ctcatcaccc gcacggtcga ttacttttat tgaagcttta 124920 gctatctgac tttctctggc atcatctgct gaatgcactg cgctactggt cgagagtttt 124980 gtataaatct ctttgtgctc tggtgagaat ttagcaaggc cagttggttc gttccccgaa 125040 tacaatgcgt taatagctgc gcgtttagaa aacttggttt taacaacaac tgcctggtct 125100 gtacgttttt cttcaacctt tgcaatagag gccgcaatag cttcattctt ggtaacagtt 125160 tcgccggtgg ctttgtctac ataaacatca ccgactttcg attccacttt tgtgtacaat 125220 tcggtattga tgccaggaat tccggcgata gattcaattg gtttggattt acgataaatc 125280 aatacgtaag tatgtttacc cgtgtactga tacatatcat ccagaacttt gaactgaccg 125340 ccggcacggg ccataataag ccgagcaaga acacgctgaa tagttcgttc ttgtcccttc 125400 attttgcggg tcgggaagcg gaacataatt gcatccattt tcatcagttt tacttgttcg 125460 taaactacat caaaaatgga tttaattgct ccaatcgggt tatcaccaag accaccttta 125520 agaatagctg gagtaccgtt tgaatttaat gacatcaaaa taacgtgtgc atacttatcg 125580 ccagttttga cctgtttaat agcatcgcct tcggatgcgt aagacaccat acgagcaata 125640 agattatcat tccccgggac ctggatattc caaatctgtg gaacttttga ctttgaaagg 125700 ttaatggccg ggatattaga ttccccttca aaaacctctg acaataattc ggtcatattg 125760 ttttccttat aaaatatgat gtatttatac gaaaaagggc cgaagccctt ttgttatttt 125820 aatccagtct ttgaccagtc gccaaataca tcttcgaatg tgtctgcatg gtctttatcg 125880 aaacggaacc ctttgataat cggcaggaac aatgcaaccg tattatcttt acgagtctcg 125940 gaagtcaccc aaccattaca ttcacagtca gcaatcattc caaccaattc acctttacga 126000 gctttaacca tcaaggcttc acggtccagt tcgtgacgtt catcaatcgg aatagaaacc 126060 cattttttgg ttttcttatc tttcacttga gttgtgtctt taaaaccaga cccacagtta 126120 tttgtaatct tgccacaaga agaacgaagc acaacaccgc caattttatc tgggtcttta 126180 tcgtgctcgt aatatccaat gatttccatt gcaatgtcaa ttacttcttt gaacttatac 126240 aagttctttg agcgcttgtt ttcccaaatc gatgccatat tcttaaggat aataccttca 126300 agaccttcat caacatattt cttatagata actttcgctt catcgagatt gttaacaaca 126360 tggttttcaa tcagaatcat gcggtcgaaa ccatgcagca ccacattatc tgcaatcatc 126420 ttttccagaa cagcaaaacg cacatcgtat ttcaacggat tgagaatctt aacatcagaa 126480 tagacttcaa tcagcggaac ataatcccaa acctggaatt tcatgcaaga agcttctgct 126540 gcagaaatag ttcctttcaa tgatttgttt gccagaccat ttgaaaaggt tcttgattcc 126600 gcgaattctg tgaattcttt tgccttacta agctcaggca tatcgtcaaa aaggaagctc 126660 aaaccatcat tctggggagc tattttctgt tcatgatata ctaactcgcc gtcaatcaaa 126720 acgccctcag gatgtctctc acgcgcttca cgggtagctt caatcaattc ctttttaagg 126780 aggtcaagct tgaggtattc gttgccagct cgggataaca atcgaacatc atccaactca 126840 tcgccacgaa cttctgcaaa acaccgagcg ccgtcggctt tcaattgcgc aaaggccggg 126900 aacttaatgt tcttggcaat accggcttca tcataggaag aagcgagcat ctgcggttgt 126960 tcaggaatta aacctttcca aaccttattt gcaattgata cggatgcccc gcattccagg 127020 tcacgcatca taacacgccg caggacttca acatctgctt ttttaccatc agcaatatag 127080 ccagataatt cctcgattgc agcattacct gttaatttac gagaggctaa tgtgaattca 127140 ataaagtcaa gcatatcatc gatattcagc ataccaaagc tttgagtcgt tgtcccaggt 127200 tttggccatt tcttaatata gaactggatg cctttggaat atgtcaaacg atacacacgt 127260 ttgagggttt cattgtcaga atacgattcc agaatagctt gtttctcttt agtagaatcg 127320 gttgcagcaa tttcatttaa aatatctaaa atcataatca cctcttagtc gttggctcta 127380 ttatatgcca catcttccaa aagcaaatcc agacgcttat gataagagaa cacaaatctc 127440 attcgcccat ctggtttaaa cttagacata aagtgaacat agataaaccc ttgctcaaca 127500 cattcacgcc aaaccggatg gtctttggct gggttaatat caaacgagta atcatgattc 127560 acatgacgaa taaactgatt aacatgacca taatccatgg tagtagtagg accataatca 127620 gaaatagtat gaacgacaaa catcaattac ccccttggcc gccggtcggt tgattgtacc 127680 aatattcaat gccagtacct acgcatgggc catattgata tggatatgct ggattataac 127740 caggagtcca tggttgacct ggctgccagc cttgttgcat ttgttgttga agacggcgag 127800 tttcacgaaa cagttcgtcc agtttacgga gttcatcagg ggtgaattct ggtttgaccg 127860 gttcatcttt gtgtttcagt ttaagctgtc cagcaacatc gtcccaatta aaaactttgg 127920 aagctgtttt aggaatagca tcacgttcgc cacgggccat ccaataaacc gggacttcaa 127980 ggatttcact cgcatggtcg caatggtgtg caaggtcatc aatataacaa acgacattat 128040 atttggtctt cgctttctgg aataactctt ctttagaggc tgtatggcca cacatcatca 128100 cttctttaaa tgcaccaggg aacaaagcat tcaggttgaa ttgacgattc agacgggcat 128160 caatggaatc acccagagct gtaacggcca caaaatcata ttgctctttg aggttattaa 128220 taacccttaa cgcatccgca taaggagaca aatagcgaat aaaatctgaa cagttatatt 128280 tctcaatcaa ctgtgctccc aactcttcat cacaattgaa cagtttacca ggggtcacaa 128340 atttttcatc ctgaatcatc ttaaggatat gttccagggg taaattatat ttttgggcga 128400 aataaggcaa gccagactgc catgacaaac atacgccatc aatatcggtt aaaataacgg 128460 gtttcataat gcatctctca attggttaag tatgtcaata aattcttgtt cagtcaatat 128520 tgtatcatga acagtaaggt ggctcgccat cgaatgcttt aaaactttgc cctttgtagc 128580 ttgtaatgcg tcgcggaatc ctttgttttg gagcgctgct tcaaaatatg catttgtata 128640 caactctttc catgatgtag agtatcttga aaagggaatt ccaagccaga aaagcgtccc 128700 acggtccgca gctcttgcat aagctcttcc agtttgctgg gcggtcaatc cggacatccc 128760 aaatatacgt ttttgttgtt cacaatttct caccttacac ccctgtagga agccttcaag 128820 acctccaaac tggatgccat ccataacaaa aggccattta gcaaagttac ttaatgcaca 128880 tgacggccat gggaaattgc ttctgatttc taactcagac atctttgaca ctgataatat 128940 ctacatcagc ccaatgacca taaccaggta gacggtcttc aatagataag ctgttatgct 129000 ttttaaggat aatcttttta ataacttttt ctttgttatc ttgaagataa ggactatggt 129060 caataactga ataagtaact tcaaccattt ctgttcccaa aattttctga atcagtttct 129120 taaacatatt atcctcgcct taaacatttg attacagaca gttcaccgct catgcgaaca 129180 tcttcagttc catcaaccca caagactaaa tacgcttctt taattacaat accaccgaat 129240 ttaaaagcag gcaggacctt tgaaatcatt ccaggaatac ccacaccttt taactgaaca 129300 gtttgtgata agaatagttg cattagaacc tcatctggaa accgtgtgat ttaacattac 129360 cgccattaat atcagacgaa ttaatttcac gtgctgcaat tgggtcaata tcaatgtcgc 129420 ggctaagctt ggtgattgcc agtgtatcac gtccattaac agccctgaat tcaactgggc 129480 aaactatatc cacatagttt ttaatggatt ctttagttgg ttgcttatca gcaggtacac 129540 ctggaccagc tttagagaaa acaacttcac agaaattttt acgtgtatca aagtaagtag 129600 tcataaacat agtatttgcc tcaaggggct ttcgccccat ttaagattag atatcaaatt 129660 cattaagaac tacatcaaaa attgcttcca ggtgttcagg ttttgctttg ttacccagga 129720 tatgacgaat ccaagtttta accaacagct tgcgattaac accattccag caaggatgag 129780 tccctaaatc acgttcacgg aaatcatcat ctaaagcaat tttaaaggtt gaaccagtca 129840 tagtgattga aagagtgata ccattttcaa atcgcatata aacgtagtta ggagtcatat 129900 actgttcaat ttcgcacact gaaccatttt tatgtttcca caggcaaata acttcagaag 129960 aacctgggat gcagttagaa acatatttgc gttcaaagtt gatgtagttc attttattct 130020 ccagttgttt tcatttgttg gtacaggttt ataatatcat aacctgtacc aaagtaaact 130080 gttttatgcg actttaatcc aattatctaa atttaattca cccttagctt tcattttttc 130140 aatcaaatca tcagcagtgg tgcgaccatt ttgataccat gtaacatctt cagaataacc 130200 agtttcttca tcttcatagg tcttacagcg aatcttacca agaatcaaag ttttcagttc 130260 gtaggtatca ccagtaaaaa agtttgttac cataattttc caggagaagt catcagaagg 130320 accttctgga gtcatgcaag aaacgaattc aaattgttct ttatacaagt gccattgcat 130380 ttctttagtt tcaatggctt tgtagccgtc gtggtcttta cctttgatgt atgagttaag 130440 attgattttc attttattct ccagtgcttt attgtgtaac tcagtatgga atcattatgc 130500 actggcccaa ccctgttgta caccctttat gaaaaaaaaa gactcccgaa ggagtcattt 130560 gataattgtt ttataggtca cttcagctgt gtagaagttt ttggctttca ttacaatagc 130620 ttcacccttt ggattcgcct caatccattc tctggcacgt ttatgtgctt ccagttcagt 130680 aagcaaacca gagcgttgac agccacggtc ttgtgtacca gcttcactta agaaagcgac 130740 tagataaatt gcgtcgtcat acggtttagc aggtgggatg tacaccggag gactcggtgg 130800 gacatacgca gcaggatgat gacccggaat ttcattgtat ggaccaggcg cggcataaga 130860 tgctccacgt ggtgtagtaa acgtgtgatt gtatctatcg ttcataatat tttcctagaa 130920 aaaagggact ccgaagagtc cctgaactta tgctttcggc ttaccaaagc aagctgcatc 130980 ggcacgcaat accgcacgag cacgagcctg aagtttatca gttacctgat tgattcgctt 131040 gttggacgca cgcttgtaac cagcgcgttt ggaggagcca tcaacaactt taacttcttt 131100 cttagccata ttaaattctc tttaaaatag aatgcaggac ttattggcat tgcctgcgca 131160 agccctcgaa gggaacatta ggtttcggat atttaacgac aggataacca taaacctcgt 131220 catcatacct tatagctctt atctgttgca caccgttagc ttaaaggttc cgggttcgat 131280 ttctcgagga taagatatat actgatggat atataggcct tgaggcgtac tcccgattgg 131340 ccatcacctc ggggtagcat gcacttgccg ctataagaat aacattcaag aggtacacca 131400 taaaattgcc gaggtcttaa aactataatg attcgcaaat cattaatcag acaattcgac 131460 ggctcctcga ttttagctca cactaaggca gtgaatctcc aataaattac ttcagagtta 131520 cgacaaaatg gcgaacagct ttacgggcag cagatgccag cggcttagca aatttcagtt 131580 catcttttgc ttcgagcact gcacgaaggt cggtttgaat cgggttaaga tgtttgaaat 131640 actgcagaat ttccagtgct tcggcttcaa catcaataga tgcgccgtag ttttcgtgac 131700 cgttattcca tgcagtgcgt tgcagttcaa gagcgtgttg taattgtttg tttaaagaca 131760 ttttagaagc ctcaatatga ggtgaatatt tggtggacac gttcttctga atttcacttc 131820 ctttcggcaa gtctctcagt tgtagtccac cgtttaggat tctccggtta actaactggt 131880 ccggcagcca atccatatta ttatttataa cgctaatttt acttataaag acaaggaaca 131940 gctttataat gacaggtcac aaatttctgc ttaacttctt tagcctgctt aagtcccaga 132000 gcaaccagag gatgtggaac gtttgcaatc ttgccaaccg gaagaggagt caaatcaccc 132060 acttcacaga aaccttcggg aacatccgga ccaaccgaaa atacttcgca catctctggg 132120 atttcaccct gatggagttt tccgataacg atacctgatg ccgtagtttc ttcatcacca 132180 gcttgctttg gctcagaaac caaaataata tattcaccta ctgctttaat tggtaattcc 132240 atatttaatc catgttgttt ggtcatgtat taataataac acgcaccgtt tgaagcatta 132300 aaggacaagc agagtctcgg tctgcagacc attcacggaa atcaatttat tgaaacagaa 132360 tgaacgccat tcattaagct tggtatcaaa tacccggata tagctaactg gttctttatt 132420 ggcttctggt ccaggggtct ggatttcttt atgcggcagc aggtccaggt cacgagtaca 132480 agtcatacgg cggatgctac catctgcttt ttcaaacaga acttcgtgaa aaccaacaga 132540 caaaatagtc ttgacttttt cacggagacg aattgtttct tgttcagtta aaatcataat 132600 tattccaaaa cagttttaat ggttgttgca ttacgctctt ttagagctct taataggcta 132660 tggcaatttt caagaatagg caccgtttta tagttcattt cggggcgaac ccactcggat 132720 ttatagtacg atttccaacg agagaaaaag tactttttat attctactga ataggctacc 132780 aggttttcac cttttgagtt cagacctgaa aacttaacta gacgaaattt cattattcac 132840 cacaaaattc gttaatgttt tcccagttca atttgtctag actacgctta ggcacattga 132900 acacctcaat tcccgcgttc ttcaggatat catcccaacc aggaatattc ttatcatacg 132960 tttcacagta aaccaatttc ttgattcctg attgagaaat tgcctttgca caatcagggc 133020 aaggagaaag cgtcacatac atagtagcgc cttcaattga cgaacccatt ctggctgcga 133080 acagaatcgc attaagctcg gcatgaattt cgttgttctt tgaccaggcc gaatgagctt 133140 cgcgatgagc tttcgccaat acaaaacggt caacctgact gaatctcacg cattctgact 133200 tatgaccagg gatgagaact ggtttaggtt tattcagtaa ccaaccttgc tcttccgcat 133260 gctcgcaaca gttaacccca cccgcagggg aaccattata ccctgtagaa ataatgcgac 133320 cattcttttc aataacagct cccactttcc aggagcaaca tttggattct tgagatacaa 133380 ggtatgcgat ttgcagaaca gtagttggtt tcatttacat caccacgaga taattggttt 133440 taatagtttc aacacgatat aattcatgtc gcaatttagt aatgctctta acatgtgttg 133500 taattatact atcaccatcg ctgaagcgtt ttttcttatc gtcaaaaatc accccaaaca 133560 ttcgctcacc aatttctaca ggagcttctg tgataacaac cgcatcataa ttacttgggt 133620 gaatactttt aaacagagca tcattcgcaa gcgttacttt gataaatgca gctgtctccg 133680 ggtccaactt acgatggtca cccaaatcat ttttaagtgc ttcctgaaaa atatccatta 133740 aattcatcat tgaatataca ctcgctgcgc ttcaaccaga ccgtcagaac ccaatttcag 133800 ttgggtcaac tgatcaccac cttttagagt aattttcgga ttaatgacaa tcataaaact 133860 gcgtgcagat tcctgaataa cttgcaaagt cgcagcaggg aatcgctctg atacgtgatt 133920 aatcatcgct tgagcaaatg ccttcacttt ttcgtggaac tcttcaacag taattggggt 133980 ttcgctaagc atcagattgt ctcctgtttt acttgtttaa tgatagaagc ttttgtgata 134040 tcgcctttga taatatagca atcttctgca gtaccacggt ttaacagacg ctctgcttcg 134100 ccttccgctc gctcttttga cacatgacga gagttaatct caatcttgcc atttttaacc 134160 acaagcaatt tccaaactga tgcaagtttc actggcacag gttcaacttc tggcttaaca 134220 ccaacttctg cccggatgaa ataatcaaat tcatcagaca atggaccagc agaaacaaac 134280 attgctccat gatgcctgtt ttctgcgcca gatatttttg ccaaatccac aaaatcattt 134340 tcatcaatca aacgtatctc acaaactttt cttccaccgc tcgcggtatc aggtacaatt 134400 ttcacttcaa acggagtcat accgatgtat ttggcaattt ggtaattagc tgcccagccg 134460 ccggcaataa actgtttttt ggcattttca gatacgaatt tgtaaaactt gccttcttca 134520 aagaaattac tcattttgtt ttcctcatgt gttgatgaaa ttcattatac accatccgtg 134580 gtgaaagtaa acacttaaat tgcttcgaca actaccgttt gtttctggaa gtccatcttg 134640 catgaaatag ccaaaatgtg gttatcaatt ttaaatggaa caattgcaaa tgctgcacca 134700 ggcgtgagtt gaacagtgat tgcatcaact gcatccggga acatgatttc cagaatagca 134760 gacaaacggc catgcacttt tagttcagtc gacacgtcga cgctttcgta tagataatca 134820 gatacaatct gactaaaaac tactttaaca atttcagaat atgtaggaaa catttctttt 134880 ctcgcttagt tattagggta tccaaaggac ctagacccca ttataacata aaatttttaa 134940 agcgttaatg gacggtatgc accttgacat taacgataaa tcggtcaatg acttcagtca 135000 gcggaacgaa ttcaacataa tattcgccct tgtatcgttc gttgatatca ttgcgcagtg 135060 cagccaggtt gttgataaga gcaggagaca tattctgccc taccagtttc tgaagctctt 135120 tataagcttc ttcttcaact tcatggtgtt tgttatacat aatgttcttc caagaaggcc 135180 aagagttctg gagcggattt aaagataccg ccatcgacct tgtcttcatc tttatagtcc 135240 attacatcca gaccaatacg tccatctgtt aaaggccata cgccgaagaa aaagcattta 135300 cgtttttcaa tttcttcgat tgtacgaaaa atcttttcga tattattcat agggttccac 135360 atctccacta cttgagtata aatatagtat atcaccaaag aggacataca atgaattatg 135420 ctttaatata tgagaattta atgaaccgag ccaaatctag aaaattgact ggatacaaag 135480 aatctcacca tgttgttcct caatgtttag gcggaccaga tacaaaagaa aaccgagttg 135540 atttaacgcc ggaagagcat tatctagcac atcaactatt ggtaaaaatt aatccagaac 135600 ataaaggatt gaaatatgct gcctatatga tgactatatc accagatggt cgccgacaga 135660 ataataaatt attcggttgg ttaaagagag attacttaaa caatagaatt caatcatccg 135720 gaatgaaagg gaaaaagcat aaacccgaat caattcaatt gatgaaagat aaacgtgctc 135780 tccaaaaaat caccgaaaca actaaagcca aaatatctaa aacaaaaact ggtgtaaaat 135840 ttacaaaaga agcactaaaa tcatttaatg aaaaacgttc cgcaaaccaa gcatggattg 135900 attccaattt taggccatgt aaagaaagca ccaaatctaa aattggcgat gccaataggg 135960 gtcgtgtttt tgataaaaag acctgccctc attgtggtaa aactggagca ggccctaata 136020 tgaaacggta tcattttgat aattgcaaat caaaagtcgc caaatgcaac ctgccagcat 136080 tcaacaccaa tacgacgcca catctcgaca acgttttgcc tatcgtcaac ggctaatttc 136140 acatcgtaat gtggggcaat tttgttccag aaaatttctt ccttcacaat atcgtctttg 136200 cggtcgtcgc cttgttcgcg ctggatgtga gtttcccaag gaattccata cttatccatc 136260 caggctttgg tggcttcata atattccgtt ggattttctt cggtaccaga ttcgcgcccg 136320 gatactgtaa taatactata ccctgcgtga tgaagcattt tcaggtactg aacaaccatt 136380 ttgtttggtg catcagtaga cagttttgca agctcaaatg gtccacgagc cacatgcaaa 136440 gccaatgttc catccaggtc aaagataatg gcttttggtt gaccaggagt tcctttataa 136500 acaggaagac caaggaaggt acgcatatga ccatacatgg aacgcagaac atcaattggt 136560 accgcttttg gaccacggcg attgttgcgt ttaaccagtt cagtccaagg aacatcaaat 136620 actttatatt caactttcca gccgtattct ttagcaaagg tttcccacat caaacgacgt 136680 tcaggattca agttggtgtc tgaaataatt acacctttaa ctgaatcgcc gccatacaaa 136740 atgttctttg cggtgtcaaa ctgcatacca gatacaatac cttctttctt cttggtgtac 136800 ttgtattcgt cgcgttcttc atgacccata atggattgac gataatcatc gcggttaatg 136860 ttaaaataac cagggttctt agcgatgaat tcacgagtcc atgtgctctt accagaacca 136920 gggcaaccta cagtcagaat aattttcttc atcatttaat tcctaacaga gttttaagaa 136980 gctgaatacg caattcaaca cgcccttcat ttaattcagc tactgcatta ttgcttgact 137040 tgcgacgagt attggcggag atgaacataa ttaaatgctc tttaagttcc cgcatatcaa 137100 cacctttcgc ttttgccgct ttgcgtagag ccttgcctgc atcatctaag gcttttgcag 137160 ggtcttcgtc attcaatacc aatccacggt ctaaatccat atatacagcg ccgcgacacg 137220 aatcaatata attatctgaa cacttgatgt aattctctaa taactctttc ataataattt 137280 cctcaaattt tataatccgt gggagcatta tactctgctc ccataagttt gtacactact 137340 ttccaaaaat accatttata cacatgctaa gcacgattcc aaaacaaaag catgtaaagc 137400 caaacagaat gacatccgac ccatcgatac aagactcaat catttgcact tctcctcttt 137460 tagttcagaa cgataataac ataacatgcc tttttggtca tgtacatacc tttttacatc 137520 attcatccaa atcctgaatt cttgcgatgc ttcaaaaggc ataccaaccc aggcttgacc 137580 ctcaattacc ttcacttccc attgctgttt gtaatcagcc aatgcaacag gccaattagg 137640 atgaacttgg attgtgggct ccacaacggg cttctgggcg catgaaacga gtccaatagc 137700 caaacatact gccaggatag atttcatcat ttggtgagct cctggaagtc ctgggcgaac 137760 ttatcaaatg actgattaat ctgtttttcg actaatttag gcttcgctgc cacaacctta 137820 gtacgttttg catctgcgcg aagtttatca ttttccttct gcaggtgttc aacatattcc 137880 tgtcgctgtt ggtcaaggag attaatttga ttaacctggt cttttactga ttgcagattt 137940 ttcgcattat cttctgcgac tttggtgact tccgccaccg ccgtcttgag attatccact 138000 tttgctttta gatagaaacc actaccagca gttactacca gggcgagcat aagccctgtg 138060 gtgagattac ttatttgcat aacttgatga ttacctcaac aatatcatcc tgggataaac 138120 cattcaggag gatgtgattt gtactgtcag atgctgaaat gcggaactga ttaccaactt 138180 catccgccat atcatcctgg gttaacactg ggtgtttaat tcccaggcgg tgttgaagtg 138240 tcaggggttc aaccacaaca taacaaacac atccattgat atgcacatta ggttgggtct 138300 gattgatgaa cacttctgca tcatatttaa ccaaatggtt ctttaagaag tcaaccattt 138360 cgggaacaga ttcatgaagg gcttctttcc gctcttctga tttcttatca cgatattctt 138420 tttgtttcgc cttcaactta ttatcagacg ccagtttctt tcttaagtca gtaaggaacc 138480 caacattttt ggaaccttta taaacataga ttccgtcatg gcggtcacca tatacatcaa 138540 caatcatatc atttgaaatc agttgcatat tcataatatt ttcctcatta gtagcggttg 138600 taacctctag gaccaaaaga gttggcaccc aggcgttcat ctttcttttt ggctcttgct 138660 ctaaacccag aatcacctgt ttgagctttc agttctttta acaatttggc cttcaggtca 138720 gtaatgaaac caatccggcg gtctcccttg tagacataga ttccgtcatt actgtcacca 138780 tatgcctgtg taactctttc atttgtaatt aagtttaaca tgttttagtc ctcatcagta 138840 gttgatggaa ccattatgta tagaaagaag gggcttgtac accccttttt attattcagg 138900 aaggattact tccgcttggt agtccgacgg aatgtaaagt ttatgatttt taaggaaaac 138960 tttattgatt tgagcaatcg tttgttcagt ttcctgcggg cgttgatagg tgttcattaa 139020 tacggagaac aatcccgggt acggtttaca caactcttga cctttaatcg caaactcgcg 139080 acggtctttt cctgccagtt gtttctgggc atcaagcagc aatccaagag tgttggacag 139140 ataatccaga tgtacaatgg taaaggcatt aactttttcg cgagcataat catcaccagt 139200 aaacatggca ataacatcat ctgcgccgcc ggcaacaaca acttcaaaca gacgctcatt 139260 attattaatg gagtctttag tatggtgcag agcacaatac cattctgttt tcagcttgaa 139320 cttctgacca cttgccagct cgtagacgaa gccttcaatc tctttcatac cacgaacatt 139380 ctcaatgctc accaggtcat atgcttcaac caggaaagga cggagtgcag catctttgaa 139440 cagctcagga taaggaatat attcgccagt ttcattatga cgaacgttca gtaaaatcag 139500 gtcacgttcc tggtaaggca aaacaatgcg gttggttggt gcaacatatt ccatattgca 139560 tgtataacca gcttcagtga tttcagtcac acgagccctg aacgcttcac ggtgcggttg 139620 tttaaacacc tgcagagatt cagcagcttg ggaactttta atcgaccctt tggatttaaa 139680 agcaatatta gtctggtcca tataagtaga aaccaagcta ccatcagctt ttgcagtaac 139740 caggacaact tcatcaaggt taaggtccat ggtcattggg ttctcaccca gattaaagaa 139800 tttttccata ggacgggcag caatgcgaac tggaccattg tcatccattt caaacataat 139860 gccgcggcac tccagcgcat catcaagaag ccaatcacta taggaagcat agttatagct 139920 aaagatgcgg aacttagttt caaatggaga aacaaaatcc gtatagaaaa acttaccttt 139980 atcggattcg gcgcaaagct tcattaagtt atcatataat tcaatcattt cttcacctta 140040 tgttggtaat tccacggggg attaaaaagc ttgatgaaca tcggctcttc tagagacatc 140100 gtttcgactg acattgtgcc aagttcattg gtcattgaca gattgaaaca ttgtcgtgca 140160 tagaatacca cctttttacc ttcttgtaaa gcttcatgta tcatccggga tttgtttgaa 140220 tcacttgtct ggtcgattcg gttaatcgca gtccggtaat agttgatacg cttcttaagg 140280 ttgttcgtct taccaatata cactagttca tcatcaactg aaatagcata gataacattt 140340 ttcttgtttg cgagagtaag aatagggatt ttaccatcga ctaactccaa ctccgcgtat 140400 tttataaaac tgtattcatc agcgatttgt ttcatgggca aaaagggccg aagccctttc 140460 cttatagata tttacggaaa ccagcaagaa cattctcatc gacgtcattg tcaatctggg 140520 ctaccaggta agcagacaac tcaacttctt gtggggcaga ttgcacatta tcagagttaa 140580 gatattcgcg aatccatgga atcgggtgct tagtagattc taacacgatt tcacaaggga 140640 gaccgcattg cttcatacga gaaactgtca ggtaatcgat aaaacggtta agaatctcga 140700 cgctcaaccc agggagtcca ccatctttaa acagatgcac ggcccattct ttttcctggc 140760 gattaacttc catgaaaatg gctgcggctt ctgcttcaca ttcgtgtgcg attttaaccc 140820 attcgtcacc gtcaacacca gtttgcagct gacgaagaat atattgggtc cccttaaggt 140880 ggagctgttc atcacgggcg atgaacttca ttatcttcgc attaccttcc atgatttcca 140940 tgttcttatg gaagttgaaa gtacaagcaa aagatacata aaaacgaata gcttccaggg 141000 cgttaataac gtgcaggcag agataaagag acttcatcaa tgctcgtttg gcgatagatt 141060 cttgtttgat agctcgttcc aaacgttcgt ccgcttcggg agtttctttg gccagttcaa 141120 caaaagcaat ctggttttgc cattgacgag ttttattcag aacatcatca tagtaatgac 141180 caatggattc tgcacgcttc atgattgcat catctaacag gatttcgtcg aatactttcg 141240 ccgggtccgt gtagagattt cgcatgatgt gcgtataaga acgagagtga attgtctcgc 141300 tgaatgtcca ggtctgaatc catgtatcaa gactcgggtc agaaactaat gcggctaatg 141360 ctgcagctgg agcacgaccc tgaatactat ccagaagtga ttggtatttc aggttgttgg 141420 taaaaatatt ttgttgatgt tcaggaagct tattaaattg ggctccatcc atcatcaggt 141480 ttacttcctc tggacgccag aagaatgata attgcttttc tgttaattct tcgaaaactt 141540 tatggcgctg gatatcataa cgggcgatgc ccaatcctga accgaagaac atcggctcgg 141600 ttaaaacatc cactggagtg gtattaaaaa ctgtactcat tttatttcct caatagctca 141660 tccatgagca taattatatc aaagtttaca ggcggagcag tcttctgctt taggagcttc 141720 gatttcgtaa tcgtctgttc cggaaccatc tctagtgtta tgataataaa ggttttttcc 141780 gccaaaatac cagaagtaca tcaggtcgtc aagcataaca gacatcggaa cttttccttt 141840 cggataattc tgtgggtcat aatatgtatt ggctgatgct gactgacaaa tccatttcaa 141900 cataatcgcg acttgtgtca gataaggttt gttaccctgc ttagctagca cccatgcgta 141960 atcgtacaat cctttattgt gctcaatatt gggcacgact tgattaaagg aaccctcttt 142020 tgactcttta atagagactg gtccacgcgg aggttcgata ccgtttgtac tgttagaaac 142080 ttgggaagat gactcacaag gcataagtgc tgataatgtg ctattacgga tgccatgttc 142140 aaccaggtct tcccgcaact gcgcccagtc acaaacgtag tttggagctg cgatttggtc 142200 aatcttttta ttgtaccagt cgataggtaa ttcgcctcga gcccaacgag tgtctgaata 142260 atattcgcaa ggtccttttt ctttggcgag cttgattgat gctcggataa ggccatattg 142320 taatctctcg aacagttcat gtgttaaatc atttgcatca gcgtaggaag cgaaattgct 142380 tgccagccat gcagcatagt tagtaacccc aacacccagg ttgcggcgtt ttttggcttt 142440 cagagcttct ttaaccggat aaccctgata gtccaacagg ttatcaagag cacgaacttg 142500 aacttcagcc agctcgttaa tcttatcttg gtcctgccag tcaaaactat ccagaacaaa 142560 tgcagacaat gtgcacaatc cgatttcagc gtcttcgctg ttcacatcag tagtcgggat 142620 tgcaatttcg cagcaaaggt ttgattgacg aatcggagcc ttatcacgga tgaacggagt 142680 gtagtcattc acgttatcga cgaactgcgg ataaaccctg gctgtaccgg aacgttcagt 142740 catgaataat tcgaatacat cttttgcttt gatacgcttc ttacgaactg tcgggtcttt 142800 ttccaaggct tcatataact cgcggaattt atccgggtta tcaaaatatg aataatacaa 142860 ttcaccgcca gcttcgtgcg gactaaacag agtgatgtaa tcattcttag ccagacgttc 142920 catcatcagg ttattcagct ggacaccata gtccatatgt ctgatgcggt tttcttcaac 142980 gcccttgttg ttcttcagaa cgagtaaatt ttcaacttcc aaatgccaaa taggataata 143040 agcagtagca gcgccgccac ggattccacc ttgtgaacat gatttaacag ccgtttggaa 143100 atgtttccag aacggaataa caccagtatg cttaacttcg cccataccga ttttcgaacc 143160 ttcggcacga atcatgccaa cgttgatacc gatacctgca cgcttactga tatattcgat 143220 gattgaatta gcagtcttat taattgactt cagggagtca gccgcttcga taaccacgca 143280 ggaactgaac tgtctggtag gtgttctggc gccggccatg ataggcgtgg gcaacgaaac 143340 ctgtcgagtg gatactgcat cataaaaacg gcaaacgtgc ttcagacggt ccgttggttc 143400 atcctggtgc agtgccatgc cgatagccat gattgcgaat tgcggagtct cgtagatttt 143460 cccagtggtt ttatctttaa ccaggtattt ttctttaagc tgcatcgcgc ctgcataagt 143520 caagtcaaaa tcacgttcgt gtttgatacg agattccaga taagtaatct cttcggccga 143580 gtagcttgac aacagttccg ggtcgtattt gccttcattg acacaataag aaatctggtc 143640 aataaaagaa cgcggttcga actggccata aacatcttta cgcagagcaa acatcgtagc 143700 cttcgctgca acgtattggt aatctggttc ttcaaccgag ataagcccag cagacacttt 143760 gatgataacc gtctgaatat cgcgagtagt cataccatcg cgcaggtgtg gtttgattgt 143820 ttcgtataat tcatacgggt cgatattggt tccttcacat gaccaggaaa gaactttaat 143880 aattttctgg ccatcaaaat cttgagatac gccactactt ttttgtactt gcatgttttc 143940 ctctgttata aaatggggtc atgcttataa taacacatga ccctgaagcg gaatcgtatt 144000 tatagaagga agttaagccc aaccattacg accaaaaata aaatgccagc aatttgaata 144060 ttcattagac cgccatcggg gctgcgattg tagggtgggg ttcatagttt tccagaacaa 144120 aatcagctgg agacatgtga cgagtcaccc aatataactg catttcggtg tcccataaat 144180 ggaactgttc gggccaattg attttcagag ttggcagagt cttcggctct cggcgcagga 144240 cttctttaca ttgctcgatg tggttaagat agatatgcgt gttaccaccc atgaatacta 144300 acttaccggg cttgagtcca gtcattttgg cgacgatatg caaaagcgta ccataagaac 144360 caatatcaaa cggaaggccc aggaatacat caaccgaacg ttgataccat agcaggtcaa 144420 gctcaccatc atgtacgtta aattggtaaa gcagatgaca aggaggcaaa gccatttgtt 144480 tcagttcagc cgggttccat gcggacacaa tctgacgacg gtcggtcgga actttcttaa 144540 ctttctcgat ggtctcaacc agctggtcaa ccccaccaaa atcacgccac tgtttaccat 144600 acactgggcc cagttcacca tcaacataac ctaatgcacg accttgtgct tcaaagttct 144660 ggtcccagat agtcttttta tcagagccag caccatgagt aatttcacgg agacgttcaa 144720 cgttcgtaga accttccagg aaccacagaa gctcaccaac cacagatttg tatgccaaac 144780 gtttaacggt cgttgcaggg aagccttcag acatgtccca ctcaactttg gtgccaaatg 144840 tcgcgatagt accagtccct gtgcggtcgt cagtctgata gccaatgtca aatacagttt 144900 gaatcaagtc ttgataattt ttcatttata tacagtctct gtaatatggg ttacttcatc 144960 tattttatac caatgggttt caagcatttc acgtttgctt atatcacaca ggaagtcatt 145020 atccaactgt actgttgagt ttacgcgatg ctttttataa attttggtca tgacaatttc 145080 atcagcataa ggtgcagcgg cttctagtaa tgatttaccg ccaataacac agatataatt 145140 ttctgaagaa ataaacattt gggccggaga atctggcgcg gagatagaaa ctttttcacc 145200 agaaatcaat ttcaaataat tggcccatgt gatataacca cccgccaact caccagcctt 145260 tgtaactgga tacggtcgcg ctgggtcagc cacaacgacg tgaaagcgcc cttttaatgt 145320 agacggcagg ctttcaaagg tttttgctcc catgagcacg atagagtttt ttgtgcgagc 145380 cttgaagttc tgcaagtctt ttttgatatg accccacggg agaccatcat taagtccaaa 145440 tgcattttca tcacgaccat caactgtttt agttggagca taagcgaata ctaatttaat 145500 cataattttt cccaccatgc aatagcttca tttgtgtcat agaaaatgtc tgcatctatt 145560 ttaacaccat ctttaaaaag tgtcactgct gctttaaata attcatagtt gttcacagac 145620 atatcagctg catcattaaa ggccttttca aattcaccag cgtcagggtc atttaatgca 145680 gtgaagagcg tccagctatc actatcatga tattcaaata ctacaatcat tttaattcct 145740 taactgtctt tttaatggca gtaaaatctt tacgaatatc gagaaccaat ttgtaaagat 145800 gtttaattgt atctgtatca aaatatgata cagcgcaacg tgattggcga atatcttcta 145860 atgaatattt gctttcactt gtaccatatc caataatacc actaatgcac ttgtcattaa 145920 gcgtatattt ctgtggacca tcaatattat ttttaggata gatgtaaagg ctccaatcaa 145980 atttttgttc aagcgtatta accttaactt caagaatcag ttcaggatgt gcatcatctt 146040 tagcaaaaca agtgtattca gtaagcatta tattttcctc acgctttctt agcaactttc 146100 cagtcagctt taaattggtc aacgtcagag tgatgaatcc agaaaccaga tgagctacca 146160 tcttcataaa gaggacaacc atcacactca tctttccaac ccatcgcgca taaagtttct 146220 tcagcttttt ccagagcttc tggattgtta ccctggattg tgaagtacca tttgccttta 146280 acttctgaat ctttgatgct ctcacgttgt aatttcattt tattctccag ggttttagtt 146340 gttaactcag tatgggacca ttatgtcata gccccatttg gttgtacact actttttaaa 146400 ttttatcaag ccatttgcca agtttaatca tttccttggt ggctttttcc atacttctga 146460 acttaactaa taaaggatga gtatcaacac ccataacaag attggttgca agactgtgct 146520 cacccacaag ccacaatgag tatgtttgaa catcgagacc ataatttctg tctttatgta 146580 aacgtacacc cattacttca aattgttcgc ttgctttttc tactgcatcc aggaaatcat 146640 ctttagtcat gttagacctc aaaagttgct ttaatcaagg caataacttc cgcggcattt 146700 ttataattta cctgcacatg aatggtttgg gtatcaacat caggagcatc accatcaact 146760 tccaggaagt gacagaattc ccattcagcg agttcaaagt actcatcaac atcacccata 146820 ctattcagga tggtgccatc tttcatttca actttttcaa caaaataatc accttcaata 146880 taacacatat ccaggacttt gaaggaattg ccatgctgtt ccattaactt gaccatgcac 146940 gaattatcat caggactacc agcgatgaat tgttttttag cagcagggtc taatacgtaa 147000 aatttaccag tttccatcat tttattttcc tcatgtagtt gataggtcta taataacata 147060 atcatagacc cgttgtaaac tgctatttga aagctttttg tagaagttca atgatttggt 147120 cgacgttatt agcatccaca acacaattaa tagaaaccgc ccccgaaggt gtttttgtat 147180 aattggagta ttcacggaag caataaaact cttcttcact aagttcaaaa taatcatcac 147240 ccatgccatc atcattatag attgcaccat tagcacaaat gatttcggtt acataatcat 147300 agccgtctgc acttgatatt gatttaactt caaaccaatc accattttct tggatgatgt 147360 cgaccatact agcattcgat gaactaatat caatgaaaga tttaatacga tgtggattga 147420 actcatattt tttgccgatt tccatttttt atttcctcag ttgttatcag tagttgatgg 147480 aatcattatg ccttagctca aaggacttgt acactacttt ttgaaaataa aaaagggacc 147540 cgaaggtccc ccttatatta aaggccagcc agaaggtcgt caagaccatc atcagaaggc 147600 tctggagtgc ttggagcagt cgcagtgcgt tcagacttag caggtttgct ggaagtgaag 147660 tcttccatat ctttatcgaa atcgtccagg tcagcgccca gtttatcagc ttgtgcagcg 147720 gctttagaag cagccccgcc aagagcagcc gtacccatta ctttagagaa tttcttctga 147780 ttctcttcaa gggatttaaa taccagcaat tcattcaggt cagacatttc gtcccaaagt 147840 tttttctgga atgcttcatc attgatacca gcgatttcag actggttcat gaatttggaa 147900 tcatcgtagt ttttataacc ggaaaccagt ttggatttca gtacaaagtt tgcaccttca 147960 aaaggacaag ttacatcaat agctgtttcg cccatgtcaa catcgacttc aaccatctgg 148020 ttaattttgt ccatgatttt cttgccgaag cgatatttga atacttgacc ttcgttagac 148080 ggaacagcag ggtctttaat gaccaggatg ttagcccagt aagaagtatt acgcttcagt 148140 ttacgatatt cggattcgtt tgtgttgtac aggtcattct tgttcatgta cgcacacaca 148200 gggcagtttt cataatcgcc gtgggtagaa gtacagtttt caatatacca ttgaccgcca 148260 tttttaaagc cgtggttaac gagcttgatg aacggagatg gattttcttc attcttggaa 148320 ggcaggaaac gaataaccgc agaaccgacg ccatcagtat ctttcagttt ccattctttt 148380 ttatcgtcag aagagaaacc agaaccacct ttcagagcgt taagggaagc agccaggtca 148440 gcagggtttt tacgtttaaa catagacata tgattttcct tagattattt gatttttagc 148500 agttgttttg attacgtatt aattatactt caaagtgatt gaagcgataa acttttgaaa 148560 ttcagaatca agatggggat tccatcctaa tttgaattta tttataatgg aaataccggt 148620 cgtgtgtgta cacggtaaac cagtttctct tgccataata actggaccaa gtctggaagt 148680 tctatgcggt cctatatacc agtcataata tattccagca tttaaccaaa ctaaatgagc 148740 acctttagct attgtattag gatgttccca aggcaataaa cctttaaggt gagaatttaa 148800 tttcatattt attttatgtg attctgattt tggtttacct tttaatgaag acttaaaggt 148860 tccacgagca ctcgccttat tgaaaaacaa tggcgattta accacattat agtgttgatg 148920 gtaaaactct tcttctgaca acacatcgtc acacacatgc agaattttaa cggttggttt 148980 ttcagacaat gcctcaataa aacgagattg tttgcatgaa gaccagtatt ctttattatg 149040 tgaatcaatt ataacaccat tttcaaaagc acaattatgc tttgacccaa tgtaaaaata 149100 aggaggcgtt ttatttttca gacgagtttc aaatgtaaac caatatacaa cattcaaaac 149160 ttacaccctt taacagtctc gatgaacaat ttacgggctt caagagaatc gatatttaag 149220 attttcttat aagcatttag tttagttgaa tacttttccc aaaccaagtc gttagtctgt 149280 tcatcatgtt ggtctattat acctaaaaat gaatcaagca aacaaaacgt ttcaaatgaa 149340 ataacattcg attggagaag tttgaaaata taacttgtat tcactttagt attatactcg 149400 aaaatctctt gaagcgattt gacttcgact ttcttactga agtaatagat attctttata 149460 tcatcttcgt atacttgttt aattctttta agtctgccaa tgtattcacg ataaaatatc 149520 agagcatcag catcacttat atcgcctatc catgcatcct ggtttgcaac caggtttgac 149580 atgaatatta acgttaattc tttaagagtg tacttatcgc taagcttttc gaaaaaatac 149640 ttatcgcgac gtttttgata agcactatcg gagattcgca tggtccagtt atatttgata 149700 atatcataac gacccgaaaa atgctgtttg atggataagt acagttggta tactgattta 149760 ccatttatgt atcggttgtt atttggaggc atgcgaatcg taatcataac aaaaagtcca 149820 gcgtattagt tttctgtgta cgagacatac tcggacgaag caggttatca tcatatgctt 149880 cattcataat cttatcgacg attcccgcag ggatatatcg cgcaaaatta ccttcaggaa 149940 ttccacgttc ttccaaccag gcagttgtag cttccagata agacatttcg gttgactcaa 150000 ccatggcttc aatatcaagc ccgttttgct gcttactaat cgccccggaa ggcgattcag 150060 ttttctgtgt atcaaaatta actaaagagg acatcataga gctccacagc ttcggttttt 150120 tcatcttcaa aacgctcacg ggtgccttta tgatacagac cgagctgtgt attgaacatc 150180 ttaccgtcaa cgcctaattc ggtttttgcc ttgtctttaa ggtctttaat ttcgtctgca 150240 tatgcttcca tttttaattt ggtatcggaa gcagctttaa tcagcttggc cagttcagca 150300 ccatgagtat caggacaaaa ttcaactttc acttttttct tctttcataa tataccttaa 150360 taaaattcag caatgtttgc agttagttta gacagaccag acttaacaaa gtaaggataa 150420 actttacttt tggccgggat gacgtagtta ttatatcgtt ctaaaattga agcggatatt 150480 tccgttggga taaagtccat atcaatcaac aattggtttt cacagaatct attatattgc 150540 tcttctgtta aaagcgtttt cagcacgtca tggtcgtaat agttgagcgc aatttgttca 150600 agctcggctg cacgtgtcgg tggagtgcgt tcaccctctt cgtgagtgta ataaaaatca 150660 ccacgaacct tgatgctcga cacgttatct tttttgtcac ctttaacaac ctttgtcacg 150720 caatccataa gggcatcgcc tgatttagtt ttgacatatt ttttctgcat cggggaccat 150780 tgtttcaccc cagggaattt atgcaactgt gtaaagtcac catccgaaga aacaatcata 150840 accggatgtc cgagaccagt gagcaacttc gttaatactg caatatggtc atcagcctcg 150900 actttatcca tattcatcac gatgtaaggc atgtgttttt ccatttcatc gataatgatg 150960 tgcatggcag catgtaggcc ttcccaatcg aattgagact cttcacgagc tttcgcacgg 151020 tttttcttat aataataaga ttttgtgcgg cgccaatagc cattcttcga gttatcaaca 151080 caaataattg gaatcgtata accgagcttc ttgaactgca caatattttt tctgatggag 151140 ttcagaacca ggtgtctggt cattgcagtt gttaccttcg gaaagcctgc gtctttgccg 151200 aattcctgga aggctgcagc catgatgagc tgactaaaat cgagtaactg aaaaccatct 151260 ttttgacggt cttcttcagg gagtaaaaaa tctaaattca tatgaacctc tgttcaatta 151320 gttgactcga ttatattagc ataaaatttt taaagcaata taaatacacg tataccaatc 151380 aataaggata aagcacatgg ccgatatttt aaaaccagca ttcagagcaa catccggtct 151440 cgatgctgct ggtgagaaag tcattaatgt cgcaaaagct gattactcag ttttatcaga 151500 cggcgttaac gtagatttct ttatagaaga aaacacagtt caacaatatg atgcgacacg 151560 aggatacaaa aagaactttg ccgttattaa cgataaccgt atttgggttg ctcaacgtga 151620 tatcgcggct ccggcaggga catttacccc tcagtattgg ttagcaaccc gtactgaccc 151680 gaaatgggaa actgttgcat ctccaactcg tcagcttagc tcgggtgaat ttatcgcggt 151740 cgactcagct gcaagcttta ccacatttac attgcctcca aacccgactg atggtgatac 151800 catcgttatt aaagatatcg gtggcaatac cggttataat gaaatcaaag ttcaatcgag 151860 caatgtacct ggtcaaggta accaaaagat tgttcgtttc ggtaaccaat ttactgaaat 151920 attaatcagt aagccgttct cttacaacat gcttattttc tccaaccgct tatggcagtt 151980 ctgggaagca ggtaacgaag aacgcggaat aagaattgaa ccaagctctg gcaaatatcg 152040 tgcacaggca gcagacttta ttatgcgcca ttacacgacc gcagagaaaa ttacatttgt 152100 tcttcctaag tatgctaacc aaggcgatat tgtcaaatca gtagatatag atggattagg 152160 gccattatac cacctggatg ttgaaacgtt tgacgagtca agttctctgg gtaaacaagg 152220 ccaacacagt atggaattcc gtactactgg tgatggtttc ttcgtttata atgccaccga 152280 aaaattgtgg gtgacctggg acggtgataa taaaactcgt ttgcgtgtaa ttcgtgacaa 152340 tgtaaaattg ttaccaaacg aaagtattat cgtgttcggt aatgataaca acacctcaca 152400 gacaattaac atcgaccttc cgacgggtgt tcgtcctggt gatgttgtta agattgctct 152460 gaactatctc cgcaaggcac agactgttaa tattaaagct tcggctactg ataaaattgc 152520 atcttctgtt cagctgcttc agttcccgaa acgttcggaa tatccacctg acactgaatg 152580 ggtatttgtt gactctttga ctttcaatgg taatataagt tatactccgg ttattgaatt 152640 aagttacatg gaagatacgg ttaataacgt taactactgg gttgttgctc agaacgttcc 152700 gactattgag cgtgttgact cgaaggatga tttgacccgt gctcgtctgg gtgttattgc 152760 attggctaac caggctcagg ctaacgttga ccatgaaaat aaccctgaaa aagaattagc 152820 aattaccccg cagactttag ctaaccgtgt ggctactgaa tcacgtcgtg gtattgcacg 152880 aattgctaac actgcacagg tgaaccagga tacgactttt gctttccagg atgacatcat 152940 catctctccg aaaaagttaa acgaacgtac agctacagaa acaagacgtg ggctcgcaga 153000 aattgccaca cagcaagaaa ctgatgcagg cactgatgat actaccatca tcactccgcg 153060 caagctacaa gctcgtcagg gctccgaaag cttatctggt atcgttaagt atgttcctac 153120 taccgggact actccagcag caagccgtat aacggttggg acaaacgttt ataataaaaa 153180 tacaaccact ctggtaattt ctccgaaagc tttggaccaa tataaagctg accagaataa 153240 ccaaggtgct gtatatctgg ctactcagtc agaagttaac gcgggtgcaa caaatacagg 153300 attcagtaac tcggtcgtga ctccggaaac attaggcgca cgcagagcaa cagattcaaa 153360 ccatggttta atcgagattg caactcaggt tgaaactaat gcaggaaccg attataccag 153420 agctgtgact cctaaaacgt taaatgaccg taaagcaaca gaatcattat ccggcatagc 153480 cgagattgct acgcaatcag aatttgatac tggcactgat gatactcgta tcgcaacgcc 153540 attaaaaatt aaaactagac ttaataatac tgctcgtact tctgttattg cagcaagtgg 153600 tttagtagaa acagggacgc tctgggacca ttatacgctg aatattcttg aagcaaatga 153660 gactcaacgt ggcaccgcac gactggcaac tcaacttgaa gttaataccg gcaccgacga 153720 caaaacaatc gttactccgc ttaagttgat gtcgaaaaaa gccacagaag gcaccgaagg 153780 tatagttcgc atcgctactc gcgcagaaac tatcgcagga acaagttcga ttctggctgt 153840 ttctcctgtt agtctgaaat ggattgcaca gtccgaacca acatgggcgg caaccacgac 153900 gacccgcggc tttgctaaga tgtctgaagg cgcaattact tttgtcggta atgcaactgc 153960 aggttcgacc caggctcttg acctgtacga gaaaaatagc tatgctatct ctccgtatga 154020 gttaaacaaa acccttggta acttcctgcc gcgtctggct aaagcagcag actcggataa 154080 actggataac ctggatagta cacagttcgt caggagagat attgaccaga cagttgaagg 154140 aacattaacc cttcgtaaaa acatcagaat cgatggccag ttaactacag gtggtacagg 154200 tgagtttggt ggttctttag ctgctaattc tacatttact attcgtaata caggtgcttc 154260 aactcgtttg atatttgaaa aaggaccaca aactggaaat aacccgtttc aaaccatgaa 154320 tatccgagta tggggtaacc aattcgctgg tggttctgat acaagtcgtt ctacgatatt 154380 tgaagttggc gatgaaacat ccaaccattt ttattctcaa cggaataatc ttggtaatat 154440 tacttttagt atcaacggca cagttcaacc gattaacgtt aatgcatccg gaacattgaa 154500 tgctaatggg gtggcaacat ttggacgttc agtgaccgca cagggtgaat ttataaccta 154560 tagtgcaaac gcatttagag ctattaatgg acagtatggg ttctttattc gtaatgataa 154620 ctcgaacatc tatttcatgc ttacaaatgc aaatgaccag actggtggct ttaacggatt 154680 aagaccatta gctattagta atacatctgg ccaagtaacg attggtgaaa gcttaatcat 154740 tgccaaaggt gctactataa atttgggtgg tttaacggtt aattcaagaa ttcgttcaca 154800 aggtactaaa cctgctgacc tttattcgag aaaacctaat gccgataata caggcttctg 154860 gtccgttgaa gttaatgatt cagctacata taatcagttc ccgggttatt tcaaaatggt 154920 cgaaaagact aacgaagtaa ctggtctgcc ttatttggag cgtggtgaag aagttaaatc 154980 acctggcaca ttgactcagt ttggtaacac gctgaattca ctctatcaag actggattac 155040 ttatccgaat actgctaatg caagcaccac tcgttggact cgtacatggc agcagaacaa 155100 aaacgcatgg tctggttttg ttcaggtctt tgatggtggc aacccacctc agccgtcgga 155160 tattggtgca ttgcctgctg ataatgcttc gatgagtaac ctgactatca gggattggtt 155220 aagaatcggt aacgtacgca ttgttccaga cccagtaacc aaatccgtta aatttgaatg 155280 gattgataca ccataagagg tattatggaa agatttatgg ctgaatttgg acagggatac 155340 gtccaagttc ccgtgctttc tgaaaataac gcagtaagat ataaattgtc gattgctggg 155400 acttgtacca aatcgccaaa ctatccctac gttaagtttc aagatgaggc cttcgggcct 155460 caggacttcc agaatggatt aaaccttatt gagattgacc cagtaacaaa tacaattacc 155520 gccaccaaaa actatggctt cacgaaagac tatgacgtca tttcacaggc ctttgtgact 155580 tatattaatt ccatcccggc tggtaggatt gtttgtttga tatcacaggg gaaattgaat 155640 gcttcccaaa ctttaattga ttggttcaga acacatggct cgactgcatt tccggataag 155700 tggttgattg accaagttga tacttcgtat tcggcttttt atatctctgg tcgaaatgca 155760 atcgttaaag agcatgtgtt gtataatgac ggggtaactg ttgaggatgt attgactcca 155820 ttggaaatgg tgtatgacga ctttgctgat gtaggcggca cagggttccc tctgcgcatc 155880 gtggaagacg aaaacacata ttacagtaat agtacacaag aaataaaacg tttccccaca 155940 gactctccta tcactcctgc ggtcgattat aatatggcac ctggagatac tttttacctg 156000 aagtttcagc tgacatatga ccaggcactt aaagaccttg gaacaactca gatgtctgtt 156060 cgattcttta atggacagac gatgattcag tcaaacgatt atgatatccc atctacagga 156120 caagcaagcc caccggctgg tgcatggatg acgtttgaac gttatgtagt tgttccaccg 156180 aatgctgatg gatttacact gtacgcaagg aagaccgctg ctgcagggca aggtggagta 156240 aggaatattt tctttacaga aatcgcccga caagaaacac aagctaaatc tgcagaaatc 156300 ggtgtcaacg gcataagaat ggttgtagct ggagaaccag gaaatcttgg taatgtcatt 156360 gcacaattga atgataaaaa tgcgacaagg tccagtcaat ctggatttgt atttgcagcg 156420 gaattccgtg aaaaagaata aggaccttcg ggtccttttt cattataaat actcttatct 156480 aataaagggg aataatatgg ccgatttaag agtaggctca acgactggtg ggtcagttat 156540 ctggcaccag ggtaactttc cgttgacgcc cgtaagcaat gatttatttt acaaaacata 156600 taaagtatat acagaattca ataaaccgca agcagcagat aacgactttg tttctaaagc 156660 agcaggtggg acatatttaa acactgtagg gtttgataaa gatttacagt ttaaagacac 156720 tgatgggtat tgggttaaat taggacgtaa agtaaacaac tccccgatgt ctgctacata 156780 ttctttctca tttagaatga gcaaaggtat gggtcttgag acatcggatg gtacaccgtt 156840 tgtaattttc gacccgacta ctgttatagg tgcaaaccgt cttactgtaa tgggtgatat 156900 ccttggtcgt caaattaaag acgaatcagg tcgagtgttt tctccaggga atactccgtc 156960 aaaagcacaa gttggattaa gtgatgtgga taacgcaaaa caggtccaaa taaataatag 157020 taacatacaa tctatggctg gcgttctttc agccccgaat ttcatatcta ggaacccagg 157080 aacattgaac gaacacgttc ctcgtattga ccaggttgtt cttagaggca catctgaaga 157140 ttttggatat tattaagagg cattatggct actttaaaat cgatacaatt taaaagaagt 157200 aaaacaccag gagccaagcc tactgcagct cagttagatg aaggcgaact ggctattaac 157260 ttgcgtgacc gcactatttt tactaaatcc gaccagggac agattattga tttgggcttt 157320 gcaaaaggcg gaactattga tggagatgtt cttcaaaacg ggacatttaa ccttaacggt 157380 aaccaatttg tttatgccgg taaatatatt gaatttttac ctaagaccac cggtaatggc 157440 gcatgggcaa accagcatct gaataaagca cctatcttta cggatttgag ttcaactacc 157500 tcagtatcag aataccaccc tttgattaag caacgttata aagatggtac tttttcagta 157560 ggtacattag taagcgaagg ctcatttaga gttcattata ttgattctac agctccagac 157620 aattctaaac actgggtctt taaccgtaac ggtaatttta ttgtagattc tggcaatatt 157680 gaggtccgca caggtaatat ttctgcgtca ggcaatatta actccgctaa tggtatagtt 157740 tctgctccac aagttattac taaaaatatt attcttgatt cgaaaaattt cggacaatac 157800 gacagtcaat ctttagttaa ctacgtatac ccaggcacag gcgaaacgaa tggtgtaaac 157860 tatcttcgta aagttcgcgc caaatccggc ggcactatgt ggcatgagct ttgcactgcc 157920 cagacaggtc aagctgatga attgtcttgg tggacaggta atactccaac atctaaacaa 157980 tatggcattc gtaatgatgg acgaatggca ggccgtaata gccttgcatt aggtacattc 158040 actacagatt tcccgtctag tgattatggc aacttcggta taatgggcga taagtatctt 158100 gttctcggtg acactgtaac tggtctgaaa tatattaaac aaggtgttta tgatttagtt 158160 ggtggaggtt attcagttgc ttccattact cctgatagtt tccgtagtac tcgtaaaggt 158220 ttatttggtc gttcagaaga ccaaggcgaa acatggatta tgcctggaac caacaaggcg 158280 cttttatcgg ttcaaacaca agctgataat aacgctgctg gcgacggcca gacacatatt 158340 ggctataact caggcggaaa aatgaaccac tatttccgtg gtaaaggcaa aactaatatt 158400 aatactcaaa aaggcatgga agttaatccg ggtattttga aattggtaac tggctctgat 158460 aatctacaat tttatgctaa cggaactatt tcttctattc aacctattaa attggataat 158520 gagatatttt taactacctc taataatact gcaggcctta aatttggcgc ccctagcgga 158580 gttaatgaaa caagagctat ccagtggaac ggtggtactc gtgaaggaca gaataaaaac 158640 tatgtgattg ttaaagcatg gggtaactca tttaatgccg ccggtgataa atctcgcgaa 158700 acggttttcc aagtatcaga tggtcaagga tattattttt atgcccatcg taaagctcca 158760 accggcgacg aaactattgg acgcattgaa gctcagtttg ctggagctct taatgctaaa 158820 agtattaatg ccatcgaaaa ttttaaagtt aatggattaa gcactttagt cggcggagtt 158880 acaatgagca atggacttaa tttaactggc ggtgctaata tcagcgggcc agttaaaatc 158940 ggcggcgtca ccaatgcatt aagaatttgg gactctcgct atggcgccat tttccgtcgc 159000 tcagaaacat cattacatat tatcccaact aatgaaaatg aaggggaaaa cggtgcaata 159060 agcaaccttc gtccgtttag tattgagtta ggcaccggta cggttataat gggggataaa 159120 tctacgggcg gaccgctttt cacggtcgac aacgtaagta aattcgtcca gacggactgt 159180 agattccgtg ttaacatgga ttctgatggt attgttgtta acgcctcatc tcaagcggca 159240 tctaacttta ttcaaggtcg taaggctgat gtgactaaat ggtatttggg tattggcgat 159300 ggcggcaacg tcgttcgtat gcacaactac acatattctc acggtattgc gttaaactct 159360 gatactgtcg atattactaa gcctcttaaa gttggaaatg cccaactagg aactgacggt 159420 aatattacag gtggtagtgg taatttcggt aatttaaata ctaccatcga gaatatgaaa 159480 gccgatattg ttaccagtta cccagtcggt gccccaattc catggccaag tgattcagtt 159540 cctgacggat ttgctttaat ggaaggccag acttttgaca caggtgctta tcctaagctc 159600 gctatcgcat atcctaccgg tactattcct gatatgcgtg gacaaactat caagggtaca 159660 cctagtggac gggccgtgtt aagcgcagaa gcagatggtg ttaagtctca taaccactcc 159720 gcatcggcat caactactgc tttgacaggc acaaccaatg gtacggactt gggtacaaaa 159780 actgtcagca ctgttgatat agggcgtaag tatactaata acgcaggagc gcatactcac 159840 acgttctcag gaacaactag tacgaacggc gaccataacc acccagcttc acttggtaac 159900 aacgccaacg ttcaatcagg tcgttttgca gcatctaact caggtcagtc tgctatagca 159960 tataccaaca acgctggtaa ccacagtcac acgttctcag gaactacgtc tgcaggccca 160020 gagcacagtc actacgttga tataggtagt cataaccaca ccgtagcaat aggttcacat 160080 agccatacgt tctctattgc ggctcacggt cataccatca ccgtaaataa caccggtaat 160140 acagaaaaca cagttaaaaa cattgctttt aactatattg ttcgtttagc ttaaggagag 160200 ggacttcggt cccttttaaa tatgaaaatt tatcactttt attttgatac taaagaattt 160260 tacaaagaag aagattataa acctattaag ggtttaggtc tcccggccca ttccacagct 160320 aaaaaacctt tagaacctaa agaaggatac gcagtagttt ttgatgaaag aattcaagat 160380 tggatttatg aagaagacca ccgtggtaaa gacgtttgga cttataataa agaacatctt 160440 attataaatt ctattggaag tttacatggg gtcacatttg acgagcctgg cgaatttgat 160500 atatggactg ataacggttg gaaagaagac gaggcttata aacgagtaac cattcgaaat 160560 aagaaaatag ccttactaca taaagaattc caggtattaa gtaatatggt tgaagcctct 160620 gttgctgata agaaagaaaa attctatcac cagaatctta aacgtttctt tgctctttta 160680 gaaaaacacg aacacttagg tggtgaattt ccggcttggc cagagaaaga gaaaaaatcc 160740 tttttcaaaa gattgtttaa aaaataatag tgcaaaggca ttataaatat ctttaaagga 160800 gaaaagtatg gaaccaaaag taggattttc attatcagac ctactttttg gacttcttga 160860 tagaattttt aaagataatg cgaccgggaa agttgttttt tcccgggtac tagttattgt 160920 tctattattt ttaatggcgg taatctggta taaaggtgat gcaattttag atgcgtatag 160980 ggatgcttca tacgcctcgt acacagaaat gataaggcaa gaccaggata acagatttaa 161040 aattgcagct atcgagcaaa tccagatagt ccactcgtca agcggagcag actttacagc 161100 gatatattcc tttaggccaa ctaaccttaa ctactttgtg gatatggtgg ggtatgaggg 161160 gatattacct gaatcagttg atgaaaaaaa tttgggcggg tttccaatag ataagacttc 161220 agcagaatat ttggcgggag ttaatggtaa ttattttgaa tccagtacgg aatctgtatt 161280 cttaccaaca aagaaaaaaa gcagcttcgc atatatgttc agctgcccat tctttaattt 161340 ggaaaacgtt tatgcaggca cagtctcgct gtactggtat aagcaaaaac ctaaccttgg 161400 ttttgatagg ctttctgcta tgtgtggaca agcaggtcgt acgctcgggc gtacacgtta 161460 gaatccggag gtatacatca taagataacg gtgtatatct tcgaatcctt cgttaaattg 161520 ttcaatgatt tgttcaattt catcttcaga taaactttta aacaacttgt taaacgatac 161580 attgtttaac tcattaccat tctcattcct caatcccaac tcattcaaaa acgcgataaa 161640 cacatcacgc ttcattgtaa tatcttcccg gtcggtttta atcaaaattg aagttacagt 161700 aacaatttca gaaacgattt caaatttagt cattttaatt tactcaagta ttcaataaga 161760 cattcaatta ttcagtaagg atattatact attattcctc tgggttgcac acatgatttt 161820 ccatctgtac aacatattcc tgatacatct gttcaactgg ttcctggaac ttaaaatcag 161880 ctccaaggaa ttcctcaaag caagtctgtt cgcgtgtacg ctgaccagtg atggtgttct 161940 ctaagttatg actcatagtg attccccttg tgtttctgct tgcgtttgga ctgcatttca 162000 gttttcttct tgtccttatg gacagacgcc ttattgaaat cgtgtttagc taccaggttg 162060 ttcatgcaat ttccttaatt ggtattcaag agagctaata atttctttat tagtcgcaat 162120 atacatatca cgcaggaagg gcttcaggcc ttctctaact ctatatgtgt tatcactaat 162180 atcctttata tattcatggt ctagggcatc ccaacctggc tggttaacat aagttcccca 162240 gttgtcttcc atcatggtat tcagatgttt gatatgatta atatggtttt caatcgcatc 162300 aatttggagt tgtttaaggt tcattagtaa aggtcctctg aataaagttc tttctcactg 162360 ccaccacgtt caatgcgcac ctgatttgca taagtcgcaa taatcattgc ttcttcgcgg 162420 gtccagtaat tgctgtattg gtcaataaat ccttggtcgt caccacaaac atggtctgac 162480 acgagtttat cacttacctg gtcaagcact tctgccatat ccttggaata atgtctggcg 162540 ccgggaataa ccagagtccc accatctttt aacttaaagc ggttggcagc acacaatccg 162600 tcgctgatat ttttcattgt tgttccaatg agcaatctgc caacagattt ccgggacttc 162660 ttctaaaaca tcctcttctg tatattcggt gtaatcacca tatgcctgta gtttagcagc 162720 gaggctttcg ggagtctcac gcgaaagagc tttatctaat aaagccatac ggtctttaaa 162780 ggataattct ttacctttta atggattcat ttgataatca ccttataacc attatatttc 162840 acgaattcat gaattttatc accaatacca atcaactcgc ctttgtcaga agaagtcaat 162900 ttgatatcaa ctgtttggca tacaccatac gaagttatgg atactttttc tatagtaata 162960 agctcaagaa atcgcaatag ctgcatacca aactcaagag gaacattatc aatctggaga 163020 tgaaggttat aaattacctt aataccattc accaaggcac agcgctcatc ccaagcgtta 163080 cgttgctctt caatcaattt aagggaatgg ccttcaccat cccagtttag ttccatagga 163140 atcatttgaa ccaaccttta acacgttgcc acagactttt ctgttgagct ttattaacac 163200 caattgagcg aataactggt tgtgattcct ggtattcttt atagtcagct ttatatactt 163260 catatgctgc tggaacaaag gcaccaatcg ctgccatgaa gttcttgcgt gcaccaaacg 163320 gagctactgc atcatcctga cgatggataa gatactgacc agctttgacc ttgacaatag 163380 ttccaaggta agcgccatga taccaaacat cccaaccctc ttgagtaggt tctacacaag 163440 cgcgcaattc attaacaata ttcagcttat tcataatttc ctcactttaa gatgttaata 163500 acatgttgaa tgttttcagt tgacataacg ttgtcaatgt aggtattcaa tccacctact 163560 ttaacgctaa atcccacgtc tgtaaactgt tttactatct tttgttcagt attatacgca 163620 tgcacgcgaa tctgaccatc gcgacgaaca tcaatctgtt ttaatgtctt gacacgcttg 163680 aacttcacaa tcagattgct gcggtcttct gaaatgctct taaagtcaat accaatagac 163740 aacaattgct ctttgatata ttctgcagtc gcttccattt catcagtcac accgcgtttt 163800 gttcttgtct tcttcacttc cagaaggtca ggattatttt cattcgccca aagttcagct 163860 gcatccagaa taaggtcaac acccttttct gtaatcatca aaccatcacc agaagcttca 163920 acatagttgt tattcaacaa ggtctgcaca ctatcaattg atgtgttctg ctggtctaaa 163980 acaacctctg ggctcacgaa gttgtttttg tatacaaaca ccagagcatt gacaacagtc 164040 tcatgcagcg cgttctggga gcatttaacg atataattta acttgttcac aattatccct 164100 taatagattt catataattt gatgcatacg attgtaccac agtacgtttc atatgggaag 164160 tagaaacaat cacatcaatc gcttgtttac gttcttcttt ggagtttgcc agtttgaaca 164220 tgtcaaagta gtaatagcaa gcttctttgt tagatttgaa tttagcgttg atgtattctt 164280 gaccttcatt ctgttgtgca gtaaacattt tattctccaa agtttttgtg attcattgtg 164340 taactcagta tggaatcatt atgcagcggg tcaaggggat tgtacactac ttttttaaaa 164400 taaaaaaaaa aggaccccga agggtcctag tctttatcta tcttattacc aataaacata 164460 ccaacaatca cacctgcgat tgccgcaaaa atacaaagca atgcaaactc aactccaaaa 164520 ctcatagagc ctccaaatca ttaaggtatt cagttttaac atcagttgtc ttccagtatt 164580 catgctcagc tttcttagcc tttgcttcgt ctgcaagctt cttggcttca tcagaggtca 164640 tatggtagat gttcatcgcc accaacttct ctgcataatc gccatatatt tcactcgcct 164700 ggagttcctc aatcaacttc ttacgaggtt tcccctggat aacaacctta ccgtcaatca 164760 cttctttaat aaagaagact tttgctcggg ccagtttaaa tgctgcttca gtttctttaa 164820 tcttactgtc aattcgcact tgaacataag tcttacggac atcaacgaaa tcgcgaatca 164880 ggtcaagcgt gttggtgtat tcagccagtt tgcctttgtg attaagcacc gttaagttct 164940 gggagagctt ctcaatcaga ccaaagtctt tcatgatttt ctcatgattt tcttcttcgg 165000 taccatccag cttgtattct ttgcggaatt taatcttaaa cccaaaacca tgctcgccgc 165060 agttatcttt ccaggtgata aaacctttct cttccagcgg gtccaggatt ttacttgtat 165120 acttttccag gttccattgg accgggattt cagtaatttc catttgggtc ctggaagtca 165180 ttttgtacgt gccatgaagc tcatacttac caggctcttc cagctcaacc acttcaccac 165240 ggaattctgg atattttact ttcggagcaa ccggctcacg accttccacg atagctttaa 165300 cagcttcctt gacgtcctgg aacgaatgag gaagaatatc agtagcgaag ccaacagcaa 165360 tcccagaagt ggcgttaagt aatacagtag gaataacagg aagataaaaa gcaggaggct 165420 tatgttcttc atcaggatgg accggagcat attcggtatc cttaaacaag ttataaaagt 165480 tctttgaaac ccttgcgaat acataacgag ctgatgatgc ttctggcacc attctggaac 165540 caaagttgcc ttgcccatca aacatggggt attcattttt atatgttgcg accagaccag 165600 aagttgcttc ttccgctgat gcttcaccat ggtgataacc ataatcagct acgccgccag 165660 cgatggatgc aaccttatgg aatttatttt tatcaccgcg tgccaggtct aatgctctcg 165720 ccataacaaa tctgtgaact ggtttaaaac catcaatcat gtttgggatg gcacgacctt 165780 ccacagtgta gatagcatat gacaatgctt ccacatcaat aatgtctttt aaatcacgtt 165840 tattcagttg catattttta ccatatcagt gaatgtactg ccattatagc atcagaaata 165900 aaaagcacaa cctgcactaa tgcaaacata actccataat acagcgctac caataaggta 165960 gcaagggcta acgagtagcc caagattttc ttaatcattt attaaccgct gattcaagaa 166020 atttaactaa ggcatccagt acaaaccata tgcaaataaa tcctgcaaaa aaagtaactt 166080 ggaacccata cacctgtagc aaaaagagcc acagcaacga taacagctac gagccagata 166140 aaagtaaaaa ctgattgcat attttcctca agggcgtcca aagacgccag ttaatttcag 166200 attatgacga tgccattgca tcacgtgttc attatggaaa ttactcatat taatagctct 166260 gcagaatgaa tttaaagtta tcagcaagca tgcgattcat ttctgaaagg gtttggcaat 166320 cagaactatg tgagcgagtg aacgccatcg caatttgacc tttaccataa ccagttgtca 166380 gaggtttcat tttagaaacc gggacaaaca ggacttcata gataacacca ttgttcgcca 166440 ttgtgcgaga cagttgtgaa cgctgtttgc ggatgtgact taatacagtg tcaaaccctt 166500 gttgtgaacg ttggttacca acataaaaac gtgcagcaac aatacgtgat tcaccttcac 166560 ctttgaccac gaaataaaaa cctggttggg taatagcttc tttagttgga cggcccgcaa 166620 cataattacc attatggatg taagcaacag tggtagcgcc agcattcagt acgtcacgac 166680 gggtcatgta agtagacata ttaatttcct caataagtta aagttttgtg ctccagtggg 166740 gtcattatac tctgacccca agagtttgta cactattttt gaagttgttc taaaataaat 166800 ccagaagtat aaaggcttgc attatacagg cggtcaagag acagtgaatc caatccataa 166860 gcttcacgaa ttgcattaat aacactttca taacttgtat aataaacatc accgcggtcc 166920 atagtcatgc ggaacttaat aaatttgtag aaaccagttt cagggtcctg atagaatgca 166980 aattctgcag acatcaaatt tttaaggtta acagcttttg taccacctga ataacttaaa 167040 tcaaatgctt ctgatatgct aatctgttta aagaacataa tgttgcgatt accaaaaaag 167100 attttagaca taatgatttc ctcagttaaa ttttattatc cactgggaac attatactct 167160 gctcccaaga gtttgtacac tattctacat caactgcgtc atcccacaag tcaacttctt 167220 ctgatgattc catcagttca tcaactgtga agcactcagt attattttca attgcaaaac 167280 gcttctgata aaaaggataa tccaaacagt tttcagtgcg cacaggacgt tcactattat 167340 ccattaaagg cgaaatgcga atcagttgtt gaccattacc caggacaaat ttttcaccaa 167400 ctacaatgtc tttaaaaagt ttcattgctt agctccattc caaattacat caacaacaga 167460 cttcaaacca ttatcctgga tttgtgtgaa tacttcagca gcaatccaga cctggaaacc 167520 aatcattgca atggcaccaa tgaaaatcag gataatcatt gccacaataa acttgaacat 167580 aaagtcaaag cggcgattgg atttctgaaa gtgtgaattg taattcataa tattatcctc 167640 aaaagaaata gttaaagtaa acacgcacat ggtcaatcat aaaaacaatt gggaaaatgc 167700 tcaggacaac aaacagtaca aacatgttcc aaatggcttt aagtaacttt ttcataatca 167760 tctccagtag ttgataggtt cattatacta caaccaccgg agatgtacac tcttttacgc 167820 aagtttcaat aataataaac taaaacaaac acaaattact gggatgcaca acatccagat 167880 aaaacaccag gttctgctgc tcacaattcc acctcaatga tttcaagttg attttcaaca 167940 attgcatgct gaatacattt atacacatca atggaccagg aattattata ttgcccatca 168000 atcaaacgtt gaaggtcttc gcgagttgac agatatggtt ggtctgattc ccattttgat 168060 aaaaacattg tggtgtatgt atcgccttcg ccggtgacaa aggtcgcgga tgcaacaaat 168120 tccccagtaa ctttatgctt gacatagtaa aatttaaatt tcattttatt ctccagtctg 168180 tttggtaact cagtatgaag ccattatgca ctgactccat actgatgtac actacttttt 168240 aattaattgc gcatttgttc tttaacttct ttatatgcgc caactgcatt cacgacccca 168300 gtaaaatctg ctgttccgcg atatagatag aaagcaattt cagccagatg atagacttca 168360 aattctgaaa gataaatgat aacgtcataa tatgcaactt gtccctggac ttcgttgaat 168420 gctatatcaa aaatttcatg agttggaaaa ttgatgttgt tgtttttaag tgcttgaata 168480 agaatttgtt tgatgtgatg agtagtaacg tgttccatga taatgtcctc atttgttggt 168540 gtgaggacat tattacacag ttggttcagc ttgtaaaccg gtgataggga aatttttctt 168600 ctgaacaaga acggaggtac cagccatccc cttgtgactt ctgcacaaga ccagactcag 168660 gaacgaaagc accgggtcca gcaaggaatt ttggtttaaa tctataacct ttgagagtct 168720 cttttacatc gagcataatc ataaggtcag caagcttgtg ctgcataaag ggaccaagat 168780 taatcccgta accatgtgga taatcgccct ggtggccagt atgcacggat atccatttgg 168840 acccagactg gtgggtcttg acttcgattc tcaccccttc cttcgaaact acatcaaatg 168900 caaaggaata aggattatcg taatcttcat caccgccaca taaaaaaccg gaggtccatt 168960 tagcaatagc ttgttcagcc attgtggcca ccatacatct ttgcatgact tgctcacggt 169020 cctgacctgg gtctttacgc aatgaatacc tggccgtgtc tacaatcttc tgcagggatt 169080 cggggtttaa gtcttccttt gtataaagaa aggagggcat ctttttcaga tgctcccagg 169140 ttttactctt ctgaatcatt tacgacctca tcgataccga cgattccccg gatgtgaagc 169200 tcgccataat acaaacggtc attagcatca aatgtatcag aatctgggtc agggaattcc 169260 ccaacaacta cttcatcaca ttccaaccaa ccatagtgaa catcaccttc atgcttgatg 169320 agcttaccgc agtaaactat tcccgcataa tgatgacccc ggagatttga aactttgatg 169380 tcacggtctg acacattgaa atgcttaatc ataatttatt caccaaaata agagatacga 169440 gttgccagta catgaacata tgcacccatc gcgtctaact gggcaatcaa aagaccttgt 169500 tgacgagcat cgagagtttc aaaaacaccg cccttaataa attcttcaag agcattaatt 169560 ttcaaaacca gctggtcatg ttcttcaact acacgtgctt ggaaacctaa cataaagcct 169620 cattaggggc cgaagccccg attaatttag aattgttcag ttacatcatc aaggactagg 169680 taaccacaga cccgcatttt ggaatcgtta taatcaaccg ggatacttac aacatcacgc 169740 gggtgaactt tacaggatac gatgcgtcca tcaccatgac caaaaccact tccgatatag 169800 cctttagagc aaacatgcaa cccggttgaa caagtttgtt cgtcattgtc attcacacga 169860 gagcgcggca ttttaacata ttggccaggg ctgttgtcga atgtgtttga gtggcaatct 169920 ttgtaagttg cacgaaccat tttccatgcg atgaaatggc cgtcttcggt gatttcgata 169980 tcgttcgcaa ccaggaagtc aaacaaacgc tgtacggctt tctcactcgg gttctcaagg 170040 agattctcaa ggaatggcag atagaattca aagtcttcgc cattttgcat tgaatcaatg 170100 atacgagtaa ccaggccaga gcgcagttca attccctgat agaacagact accaccttca 170160 atacgcacat cgcctttcac atatttttgt actgcagctt tgattgagat aaactcgata 170220 gcatcatcaa aacgaaattc tttgagtgcc tggataatat cattaaagtg tttatggtct 170280 tttgtggcgt tccagactgt gcgaccttca gtgatggaaa cgaatttgct tgatgcgttc 170340 catacgattt ctgggcggct accgccaatt gtatcttcaa caaccggaac cacttgaggt 170400 tcattaggaa ccgttactgt aatatttccg gtaataacta cttcagaagg cttaaattct 170460 ttatcatttt tcaaaacgcg acgaatggta tcaaccgaaa tattgtacca gtcagctagt 170520 tcctgctgtg tatagttacc tgaagcatac agagaaacaa tttgtttttg ttcgtattca 170580 gagacacatt taatattgta cat 170603 <210> 2 <211> 519 <212> PRT <213> bacteriophage SP18 <400> 2 Met Lys Lys Asn Ala Leu Val Gln Lys Trp Ser Ala Leu Leu Glu Asn 1 5 10 15 Glu Ala Leu Pro Glu Ile Val Gly Ala Ser Lys Gln Ala Ile Ile Ala 20 25 30 Lys Ile Phe Glu Asn Gln Glu Gln Asp Ile Leu Thr Ala Pro Glu Tyr 35 40 45 Arg Asp Glu Lys Ile Ser Glu Ala Phe Gly Ser Phe Leu Thr Glu Ala 50 55 60 Glu Ile Gly Gly Asp His Gly Tyr Asp Ala Thr Asn Ile Ala Ala Gly 65 70 75 80 Gln Thr Ser Gly Ala Val Thr Gln Ile Gly Pro Ala Val Met Gly Met 85 90 95 Val Arg Arg Ala Ile Pro His Leu Ile Ala Phe Asp Ile Cys Gly Val 100 105 110 Gln Pro Leu Asn Asn Pro Thr Gly Gln Val Phe Ala Leu Arg Ala Val 115 120 125 Tyr Gly Lys Asp Pro Val Ala Ala Gly Ala Lys Glu Ala Phe His Pro 130 135 140 Met Tyr Ala Pro Asn Ala Met Phe Ser Gly Gln Gly Ala Ala Gln Thr 145 150 155 160 Phe Glu Ala Leu Ala Ala Ser Lys Val Leu Glu Val Gly Lys Ile Tyr 165 170 175 Ser His Phe Phe Glu Ala Thr Gly Ser Ala His Phe Gln Ala Val Glu 180 185 190 Ala Val Thr Val Asp Ala Ala Ala Thr Asp Ala Ala Lys Leu Asp Ala 195 200 205 Ala Val Thr Ala Leu Leu Glu Ala Gly Lys Leu Ala Glu Leu Ala Glu 210 215 220 Gly Met Ala Thr Ser Ile Ala Glu Leu Gln Glu Gly Phe Asn Gly Ser 225 230 235 240 Gln Asp Asn Pro Trp Asn Glu Met Gly Phe Arg Ile Asp Lys Gln Val 245 250 255 Ile Glu Ala Lys Ser Arg Gln Leu Lys Ala Ser Tyr Ser Ile Glu Leu 260 265 270 Ala Gln Asp Leu Arg Ala Val His Gly Met Asp Ala Asp Ala Glu Leu 275 280 285 Ser Gly Ile Leu Ala Thr Glu Ile Met Leu Glu Ile Asn Arg Glu Val 290 295 300 Ile Asp Trp Ile Asn Tyr Ser Ala Gln Val Gly Lys Ser Gly Met Thr 305 310 315 320 Asn Thr Val Gly Ala Lys Ala Gly Val Phe Asp Phe Gln Asp Pro Ile 325 330 335 Asp Ile Arg Gly Ala Arg Trp Ala Gly Glu Ser Phe Lys Ala Leu Leu 340 345 350 Phe Gln Ile Asp Lys Glu Ala Ala Glu Ile Ala Arg Gln Thr Gly Arg 355 360 365 Gly Ala Gly Asn Phe Ile Ile Ala Ser Arg Asn Val Val Asn Val Leu 370 375 380 Ala Ala Val Asp Thr Ser Val Ser Tyr Ala Ala Gln Gly Leu Gly Lys 385 390 395 400 Gly Phe Asn Val Asp Thr Thr Lys Ala Val Phe Ala Gly Val Leu Gly 405 410 415 Gly Lys Tyr Arg Val Tyr Ile Asp Gln Tyr Ala Arg Ala Asp Tyr Phe 420 425 430 Thr Ile Gly Tyr Lys Gly Ala Asn Glu Met Asp Ala Gly Ile Tyr Tyr 435 440 445 Ala Pro Tyr Val Ala Leu Thr Pro Leu Arg Gly Ser Asp Pro Lys Asn 450 455 460 Phe Gln Pro Val Met Gly Phe Lys Thr Arg Tyr Gly Ile Gly Ile Asn 465 470 475 480 Pro Phe Ala Asp Pro Ala Ala Gln Ala Pro Thr Lys Arg Ile Gln Asn 485 490 495 Gly Met Pro Asp Ile Val Asn Ser Leu Gly Leu Asn Gly Tyr Phe Arg 500 505 510 Arg Val Tyr Val Lys Gly Ile 515

Claims (10)

  1. 이질균에 특이적 사멸능을 갖는 박테리오파지 (기탁번호 KCTC 11432BP).
  2. 제1항에 있어서, 상기 박테리오파지는 서열번호 1의 염기서열로 표시되는 유전체를 갖는 박테리오 파지.
  3. 제1항에 있어서, 상기 이질균은 시겔라소네이(Shigella sonnei) 균인 박테리오 파지.
  4. 제1항의 박테리오파지 또는 이의 배양물을 유효성분으로 함유하는 항균제.
  5. 제4항에 있어서, 상기 항균제는 이질균을 사멸시키는 것인 항균제.
  6. 제5항에 있어서, 상기 이질균은 시겔라소네이(Shigella sonnei) 균인 항균제.
  7. 삭제
  8. 제1항의 박테리오파지 또는 이의 배양물을 유효성분으로 함유하는, 세균성 이질의 예방 또는 치료용 약학적 조성물.
  9. 제1항의 박테리오파지 또는 이의 배양물을 포함하는 사료첨가제.
  10. 제1항의 박테리오파지 또는 이의 배양물을 포함하는 식품첨가제.
KR1020090098650A 2009-10-16 2009-10-16 이질균에 특이적 사멸능을 갖는 박테리오파지 KR101101377B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020090098650A KR101101377B1 (ko) 2009-10-16 2009-10-16 이질균에 특이적 사멸능을 갖는 박테리오파지

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020090098650A KR101101377B1 (ko) 2009-10-16 2009-10-16 이질균에 특이적 사멸능을 갖는 박테리오파지

Publications (2)

Publication Number Publication Date
KR20110041698A KR20110041698A (ko) 2011-04-22
KR101101377B1 true KR101101377B1 (ko) 2012-01-02

Family

ID=44047509

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020090098650A KR101101377B1 (ko) 2009-10-16 2009-10-16 이질균에 특이적 사멸능을 갖는 박테리오파지

Country Status (1)

Country Link
KR (1) KR101101377B1 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101992013B1 (ko) * 2017-08-16 2019-06-21 경북대학교 산학협력단 병원성 장내세균에 대해 사멸능을 갖는 신규 박테리오파지 및 이의 용도

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010043924A1 (en) 1994-04-05 2001-11-22 Exponential Biotherapies, Inc. Antibacterial therapy with bacteriophage physico-chemically altered to delay inactivation by the host defense system
US20050260171A1 (en) 1996-04-15 2005-11-24 Nymox Corporation Compositions containing bacteriophages and method of using bacteriophages to treat infections
KR20100116289A (ko) * 2009-04-22 2010-11-01 경북대학교 산학협력단 병원성 장내세균을 사멸시키는 박테리오파지

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010043924A1 (en) 1994-04-05 2001-11-22 Exponential Biotherapies, Inc. Antibacterial therapy with bacteriophage physico-chemically altered to delay inactivation by the host defense system
US7332307B2 (en) 1994-04-05 2008-02-19 The United States Of America As Represented By The Department Of Health And Human Services Antibacterial therapy with bacteriophage physico-chemically altered by pegylation to delay inactivation by the host defense system
US20050260171A1 (en) 1996-04-15 2005-11-24 Nymox Corporation Compositions containing bacteriophages and method of using bacteriophages to treat infections
KR20100116289A (ko) * 2009-04-22 2010-11-01 경북대학교 산학협력단 병원성 장내세균을 사멸시키는 박테리오파지

Also Published As

Publication number Publication date
KR20110041698A (ko) 2011-04-22

Similar Documents

Publication Publication Date Title
RU2725726C2 (ru) Бактериофаги, фаговые пептиды и способы их применения
KR101592177B1 (ko) 대장균에 대한 광범위 항균 활성을 갖는 박테리오파지를 활용한 대장균 감염을 방지 및 처치하는 방법
AU2015205512B2 (en) Phage therapy of E coli infections
KR101649851B1 (ko) 신규한 시가독소생산 F18형 대장균 박테리오파지 Esc-COP-1 및 이의 시가독소생산 F18형 대장균 증식 억제 용도
CN108697783A (zh) 包含细菌菌株的组合物
CN110691603A (zh) 包含呼吸道抗菌噬菌体的噬菌体组合物及其使用方法
CN107109373B (zh) 新型噬菌体以及包含其的组合物
KR101761581B1 (ko) 신규한 장침입성 대장균 박테리오파지 Esc-COP-4 및 이의 장침입성 대장균 증식 억제 용도
KR102073095B1 (ko) 대장균 박테리오파지 Esc-COP-14 및 이의 병원성 대장균 증식 억제 용도
KR20160080176A (ko) 신규한 장출혈성 대장균 박테리오파지 Esc-CHP-1 및 이의 장출혈성 대장균 증식 억제 용도
KR20220041204A (ko) 2차 대사산물의 생산을 위한 마리노모나스 ef1 및 로도코커스 ef1
KR102396828B1 (ko) 내열성 박테리오파지 ts3, ts6, ts13 및 이를 포함하는 살모넬라 억제용 조성물
RU2732792C1 (ru) Метод фаг-опосредованного биопроцессинга для продления срока годности охлажденной рыбы
KR102189126B1 (ko) 항생제 내성을 갖는 아시네토박터 속의 균을 용균하는 신규한 박테리오파지
CN110719784A (zh) 治疗性噬菌体组合物
KR20180042691A (ko) 신규한 프로테우스 미라빌리스 특이 박테리오파지 pm2 및 이를 포함하는 항균 조성물
KR101993123B1 (ko) 신규한 병원성 대장균 특이 박테리오파지 eco5 및 이를 포함하는 항균 조성물
KR101859974B1 (ko) 신규한 에로모나스 하이드로필라 박테리오파지 Aer-HYP-2 및 이의 에로모나스 하이드로필라 균 증식 억제 용도
KR101101377B1 (ko) 이질균에 특이적 사멸능을 갖는 박테리오파지
KR20230005365A (ko) 병원성 대장균의 증식을 억제하기 위한 조성물 및 방법
KR102016919B1 (ko) 신규한 살모넬라균 특이 박테리오파지 sc1 및 이를 포함하는 항균 조성물
WO2020139279A2 (en) Acinetobacter baumannii bacteriophage mikab48 or lytic protein derived from the bacteriophage
KR102418861B1 (ko) 포도상구균에 대한 사멸능을 갖는 박테리오파지
KR102275107B1 (ko) 항생제 내성을 갖는 아시네토박터 속의 균을 용균하는 신규한 박테리오파지
KR102722687B1 (ko) 살모넬라 사멸능을 갖는 박테리오파지 및 이의 용도

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20150807

Year of fee payment: 5

LAPS Lapse due to unpaid annual fee