KR101761581B1 - 신규한 장침입성 대장균 박테리오파지 Ｅｓｃ-ＣＯＰ-4 및 이의 장침입성 대장균 증식 억제 용도 - Google Patents

신규한 장침입성 대장균 박테리오파지 Ｅｓｃ-ＣＯＰ-4 및 이의 장침입성 대장균 증식 억제 용도 Download PDF

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KR101761581B1
KR101761581B1 KR1020140192984A KR20140192984A KR101761581B1 KR 101761581 B1 KR101761581 B1 KR 101761581B1 KR 1020140192984 A KR1020140192984 A KR 1020140192984A KR 20140192984 A KR20140192984 A KR 20140192984A KR 101761581 B1 KR101761581 B1 KR 101761581B1
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bacteriophage
cop
coli
escherichia coli
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윤성준
강상현
전수연
백형록
손지수
김병국
신희정
강동민
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주식회사 인트론바이오테크놀로지
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Priority to KR1020140192984A priority Critical patent/KR101761581B1/ko
Priority to MX2017008562A priority patent/MX2017008562A/es
Priority to PCT/KR2015/014332 priority patent/WO2016108542A1/ko
Priority to CN201580071157.6A priority patent/CN107208067B/zh
Priority to RU2017121452A priority patent/RU2662984C1/ru
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Priority to US15/538,588 priority patent/US10265356B2/en
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Abstract

본 발명은 장침입성 대장균을 특이적으로 사멸시킬 수 있는 능력을 갖는 서열번호 1로 표시되는 유전체를 갖는 것을 특징으로 하는 자연으로부터 분리한 미오비리대 박테리오파지 Esc-COP-4 (수탁번호 KCTC 12663BP), 및 이를 유효성분으로 포함하는 조성물을 이용한 장침입성 대장균의 감염을 방지 및 처치하는 방법에 관한 것이다.

Description

신규한 장침입성 대장균 박테리오파지 Ｅｓｃ-ＣＯＰ-4 및 이의 장침입성 대장균 증식 억제 용도{Novel enteroinvasive Escherichia coli bacteriophage Esc-COP-4 and its use for preventing proliferation of enteroinvasive Escherichia coli}
본 발명은 장침입성 대장균에 감염하여 장침입성 대장균을 사멸시킬 수 있는 자연으로부터 분리한 박테리오파지 및 이를 유효성분으로 포함한 조성물을 이용한 장침입성 대장균의 감염을 방지 및 처치하는 방법에 관한 것으로, 더욱 상세하게는 장침입성 대장균을 특이적으로 사멸시킬 수 있는 능력을 갖는 서열번호 1로 표시되는 유전체를 갖는 것을 특징으로 하는 자연으로부터 분리한 미오비리대 박테리오파지 Esc-COP-4 (수탁번호 KCTC 12663BP), 및 상기 박테리오파지를 유효성분으로 포함하는 조성물을 이용한 장침입성 대장균의 감염 방지 및 감염 후 처치 방법에 관한 것이다.
대장균(Escherichia coli, E. coli)은 표면항원(lipopolysaccharide, O), 편모항원(flagellin, H) 및 캡슐항원(polysaccharide, K)의 조합에 따라 9,000 여종 이상의 혈청형이 알려져 있고, 병인론적으로 발병특성, 독소의 종류 및 O:H 혈청군을 토대로 장독소 대장균(Enterotoxigenic E. coli, ETEC), 장침입성 대장균(Enteroinvasive E. coli, EIEC), 장병원성 대장균(Enteropathogenic E. coli, EPEC) 및 장출혈성 대장균(Enterohemorrhagic E. coli, EHEC)으로 분류된다. 이 중 장침입성 대장균(Enteroinvasive Escherichia coli: EIEC)은 대장점막의 상피세포에 침입해서 감염을 일으키는데, 이로 인한 세포의 괴사 등에 의해 궤양이 형성되고 혈액과 점액이 섞인 설사를 일으키는 세균성 이질이 발생된다.
장침입성 대장균은 돼지 설사의 중요한 원인균으로서 감염돼지의 성장률 저하 및 폐사를 유발하고 치료 및 예방 비용으로 많은 경제적 피해를 초래하고 있다. 이에 따라 장침입성 대장균 감염에 의한 축산 산업에서의 피해는 상당히 크며, 이의 감염을 방지할 수 있고 또한 이들의 감염을 효과적으로 처치할 수 있는 방법의 개발이 절실하다 할 수 있다. 이러한 장침입성 대장균의 감염 방지나 처치 목적으로 다양한 항생제들이 사용되어 왔으나 최근 항생제 내성균의 발생이 증가함에 따라 항생제 외의 다른 방안의 확보가 시급한 실정이다.
최근 세균성 질환의 대처 방안으로 박테리오파지(bacteriophage)의 활용이 크게 주목을 받고 있다. 특히 자연친화적 방식의 선호로 인하여 박테리오파지에 대한 관심은 어느 때보다 높다고 할 수 있다. 박테리오파지는 세균에 감염하는 아주 작은 미생물로서 보통 파지(phage)라고 줄여서 부르기도 한다. 박테리오파지는 박테리아에 감염(infection)한 후 박테리아 세포 내부에서 증식을 하고, 증식 후 자손 박테리오파지들이 박테리아 밖으로 나올 때 숙주인 박테리아의 세포벽을 파괴하는 방식으로 박테리아를 사멸시키는 능력을 갖고 있다. 박테리오파지의 박테리아 감염 방식은 매우 특이성이 높아서 특정 박테리아에 감염할 수 있는 박테리오파지의 종류는 일부로 한정된다. 즉, 특정 박테리오파지는 특정 범주의 박테리아에만 감염할 수 있고 이로 인하여 특정 박테리오파지는 특정 박테리아만을 사멸시키며 다른 박테리아에는 영향을 주지 않는다.
박테리오파지는 1915년 영국의 세균학자 Twort가 포도상구균(Micrococcus) 집락이 어떤 것에 의해 투명하게 녹는 현상에 대한 연구를 수행하면서 발견되었다. 또한, 1917년에는 프랑스의 세균학자 d'Herelle이 이질환자 변의 여과액 중에 적리균(Shigella disenteriae)을 녹이는 작용을 가진 것이 있다는 것을 발견하고 이에 대한 연구를 통해 독립적으로 박테리오파지를 발견하였으며, 세균을 잡아먹는다는 뜻에서 박테리오파지라고 명명하였다. 이후 이질균, 장티푸스균, 콜레라균 등 여러 병원성 박테리아에 대한 박테리오파지가 계속적으로 발견되었다.
박테리아를 사멸시킬 수 있는 특별한 능력으로 인하여 박테리오파지는 발견 이후 박테리아 감염에 대응하는 방안으로 기대를 모았으며 관련하여 많은 연구들이 있었다. 그러나 Flemming에 의해 페니실린이 발견된 이후, 항생제의 보급이 일반화되면서 박테리오파지에 대한 연구는 일부 동유럽 국가들 및 구소련에 한정되어서만 명맥이 유지되었다. 그런데 2000년 이후에 항생제 내성균의 증가로 인하여 기존 항생제의 한계성이 나타나고, 기존 항생제의 대체 물질로의 개발 가능성이 부각되면서 다시 박테리오파지가 항-박테리아제로 주목을 받고 있다.
특히 최근 항생제 사용에 대한 정부 차원의 규제가 전 세계적으로 강화됨에 따라 박테리오파지에 대한 관심이 더욱 높아지고 있으며 산업적 활용 사례도 증가하고 있다.
이에, 본 발명자들은 장침입성 대장균을 선택적으로 사멸시킬 수 있는 자연으로부터 분리된 박테리오파지를 이용하여 장침입성 대장균의 감염을 방지 또는 처치하는 데에 활용될 수 있는 조성물을 개발하고, 또 이 조성물을 이용하여 장침입성 대장균의 감염을 방지 또는 처치하는 방법을 개발하고자 노력한 끝에, 이에 적합한 박테리오파지를 자연으로부터 분리하고 이 분리된 박테리오파지를 타 박테리오파지와 구별하여 특정 지을 수 있도록 유전체(genome)의 유전자 서열을 확보한 후 상기 박테리오파지를 유효성분으로 한 조성물을 개발한 다음 이 조성물이 장침입성 대장균의 감염 방지 및 처치에 효과적으로 활용될 수 있음을 확인함으로써 본 발명을 완성하였다.
따라서 본 발명의 목적은 장침입성 대장균을 특이적으로 사멸시킬 수 있는 능력을 갖는 서열번호 1로 표시되는 유전체를 갖는 것을 특징으로 하는 자연으로부터 분리한 미오비리대(Myoviridae) 박테리오파지 Esc-COP-4 (수탁번호 KCTC 12663BP)를 제공하는 것이다.
본 발명의 또 다른 목적은 장침입성 대장균에 감염하여 장침입성 대장균을 사멸시킬 수 있는 분리 박테리오파지 Esc-COP-4를 유효성분으로 포함하는 장침입성 대장균의 감염을 방지하는 데에 활용 가능한 조성물 및 이 조성물을 이용한 장침입성 대장균의 감염 방지 방법을 제공하는 것이다.
본 발명의 또 다른 목적은 장침입성 대장균에 감염하여 장침입성 대장균을 사멸시킬 수 있는 분리 박테리오파지 Esc-COP-4를 유효성분으로 포함하는 장침입성 대장균의 감염을 처치하는 데에 활용 가능한 조성물 및 이 조성물을 이용한 장침입성 대장균의 감염 처치 방법을 제공하는 것이다.
본 발명의 또 다른 목적은 상기 조성물들을 이용한 장침입성 대장균 감염 방지 및 처치 목적의 소독제를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 조성물들을 이용한 장침입성 대장균 감염 방지 및 처치 목적의 음수첨가제를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 조성물들을 이용한 장침입성 대장균 감염 방지 및 처치를 통한 사양 효과 제공 목적의 사료첨가제를 제공하는 것이다.
본 발명은 장침입성 대장균을 특이적으로 사멸시킬 수 있는 능력을 갖는 서열번호 1로 표시되는 유전체를 갖는 것을 특징으로 하는 자연으로부터 분리한 미오비리대 박테리오파지 Esc-COP-4 (수탁번호 KCTC 12663BP), 및 이를 유효성분으로 포함하는 조성물을 이용한 장침입성 대장균의 감염 방지 및 처치 방법을 제공한다.
박테리오파지 Esc-COP-4는 본 발명자들에 의해 분리된 후 2014년 8월 21일자로 한국생명공학연구원 생물자원센터에 기탁되었다 (수탁번호 KCTC 12663BP).
또한, 본 발명은 장침입성 대장균의 감염을 방지 또는 처치하는 데에 활용될 수 있는 박테리오파지 Esc-COP-4를 유효성분으로 포함하는 소독제, 음수첨가제, 및 사료첨가제를 제공한다.
본 발명의 조성물에 포함되는 박테리오파지 Esc-COP-4는 장침입성 대장균을 효과적 사멸시키므로 장침입성 대장균에 의해 유발되는 대장균성 설사증의 예방(감염 방지)이나 치료(감염 처치)에 효과를 나타낸다. 따라서 본 발명의 조성물은 장침입성 대장균에 의해 유발되는 대장균성 설사증에 대한 예방 및 치료 목적으로 활용될 수 있다. 본 명세서에서의 대장균성 설사증이란 대장균 감염에 의해 발열, 설사 등을 수반하는 증상을 총칭한다.
본 명세서에서 사용된 “처치” 또는 “치료”라는 용어는 (ⅰ) 장침입성 대장균에 의해 유발된 대장균성 설사증의 억제; 및 (ⅱ) 장침입성 대장균에 의해 유발된 대장균성 설사증의 경감을 의미한다.
본 명세서의 “분리” 또는 “분리된”은 자연 상태로부터 여러 실험 기법을 활용하여 박테리오파지를 분리하는 것과 타 박테리오파지와 구별하여 특정 지을 수 있는 특징을 확보하는 일을 지칭하며, 이에 더하여 생물공학기술로 박테리오파지를 산업적으로 활용할 수 있게끔 증식시키는 것도 포함한다.
본 발명의 조성물에 포함되는 약제학적으로 허용되는 담체는 제제 시에 통상적으로 이용되는 것으로서, 락토오스, 덱스트로오스, 수크로오스, 솔비톨, 만니톨, 전분, 아카시아 고무, 인산칼슘, 알기네이트, 젤라틴, 규산칼슘, 미세결정성 셀룰로오스, 폴리비닐피롤리돈, 셀룰로오스, 물, 시럽, 메틸 셀룰로스, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 활석, 스테아르산 마그네슘 및 미네랄 오일 등을 포함하나, 이에 한정되는 것은 아니다. 본 발명의 조성물은 상기 성분들 이외에 윤활제, 습윤제, 감미제, 향미제, 유화제, 현탁제, 보존제 등을 추가로 포함할 수 있다.
본 발명의 조성물에는 박테리오파지 Esc-COP-4가 유효성분으로 포함된다. 이때 포함되는 박테리오파지 Esc-COP-4는 1× 101 pfu/㎖ 내지 1× 1030 pfu/㎖ 또는 1× 101 pfu/g 내지 1 × 1030 pfu/g로 포함되며, 바람직하게는 1× 104 pfu/㎖ 내지 1× 1015 pfu/㎖ 또는 1× 104 pfu/g 내지 1× 1015 pfu/g로 포함된다.
본 발명의 조성물은 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있는 방법에 따라, 약학적으로 허용되는 담체 및/또는 부형제를 이용하여 제제화 됨으로써 단위 용량 형태로 제조되거나 또는 다용량 용기 내에 내입시켜 제조될 수도 있다. 이때 제형은 오일 또는 수성 매질 중의 용액, 현탁액 또는 유화액 형태이거나 엑스제, 분말제, 과립제, 정제 또는 캡슐제 형태일 수도 있으며, 분산제 또는 안정화제를 추가적으로 포함할 수도 있다.
본 발명의 조성물은 활용 방식에 따라, 이에 국한되지 않지만, 소독제, 음수첨가제, 및 사료첨가제로 구현될 수 있다.
본 발명의 박테리오파지 Esc-COP-4를 유효성분으로 포함하는 조성물을 이용한 장침입성 대장균의 감염 방지 및 처치 방법은 기존의 항생제 등의 화학물질에 기반을 둔 방식에 비하여 장침입성 대장균에 대한 특이성이 매우 높다는 장점을 제공할 수 있다. 이는 다른 유용한 상재균에는 영향을 주지 않으면서도 장침입성 대장균의 감염 방지 또는 처치 목적으로 사용할 수 있음을 의미하며, 이의 사용에 따른 부작용이 매우 적다. 통상적으로 항생제 등의 화학물질을 사용하면 일반 상재균들도 피해를 함께 입게 되어 결과적으로 동물의 면역력 저하 등을 초래하여 다양한 부작용이 나타난다. 한편, 본 발명은 자연계에 이미 존재하는 박테리오파지를 분리하여 조성물의 유효성분으로 사용하기 때문에 매우 자연 친화적이라는 장점 또한 제공할 수 있다.
도 1은 박테리오파지 Esc-COP-4의 전자현미경 사진이다.
도 2는 박테리오파지 Esc-COP-4의 장침입성 대장균에 대한 사멸능을 보여주는 실험 결과이다. 투명한 부분은 박테리아가 용균되어 결과적으로 형성된 용균반이다.
이하, 실시예에 의거하여 본 발명을 보다 구체적으로 설명하지만, 이들 실시예는 본 발명의 예시일 뿐이며 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.
실시예 1: 장침입성 대장균을 사멸시킬 수 있는 박테리오파지의 분리
장침입성 대장균을 사멸시킬 수 있는 박테리오파지의 선별에는 자연 환경으로부터 확보된 시료들을 이용하였다. 한편, 박테리오파지 분리에 사용된 장침입성 대장균은 본 발명자들에 의해 미리 분리되어 장침입성 대장균으로 동정(identification)된 것이다.
박테리오파지 분리 과정을 상세히 설명하면, 수집된 시료를 장침입성 대장균을 1/1000 비율로 접종한 TSB(Tryptic Soy Broth) 배지(카제인 다이제스트, 17 g/L; 소이빈 다이제스트, 3 g/L; 덱스트로스, 2.5 g/L; NaCl, 5 g/L; 디포타슘 포스페이트, 2.5 g/L)에 함께 첨가한 다음 37℃에서 3-4시간동안 진탕배양 하였다. 배양 후, 8,000 rpm에서 20분간 원심분리하여 상등액을 회수하였다. 회수된 상등액에 장침입성 대장균을 1/1000 비율로 접종한 다음 37℃에서 3-4시간동안 또 다시 진탕배양 하였다. 박테리오파지가 시료에 포함되어 있었을 경우 박테리오파지의 수(titer)가 증가될 수 있도록 이러한 과정을 총 5회 반복하였다. 5회 반복 후에 배양액을 8,000 rpm에서 20분간 원심분리 하였다. 원심분리 후, 회수된 상등액을 0.45 ㎛의 필터를 이용하여 여과를 해 주었다. 얻어진 여과액을 사용한 통상의 점적 실험(spot assay)을 통하여 장침입성 대장균을 사멸시킬 수 있는 박테리오파지가 있는지를 조사하였다.
상기 점적 실험은 다음과 같이 실시되었다. TSB 배지에 장침입성 대장균을 1/1000 비율로 접종한 다음 37℃에서 한밤동안 진탕배양 하였다. 이렇게 하여 준비된 장침입성 대장균의 배양액 3 ml (OD600이 1.5)을 TSA(Tryptic Soy Agar) 평판배지(카제인 다이제스트, 15 g/L; 소이빈 다이제스트, 5 g/L; NaCl, 5 g/L; 아가, 15 g/L)에 도말(spreading)하였다. 도말한 평판 배지를 클린벤치(clean bench)에서 약 30분 정도 방치하여 도말액이 건조되게 하였다. 건조 후 앞에서 준비한 여과액 10 μl를 장침입성 대장균이 도말된 평판 배지 위에 점적하였다. 이를 30분 정도 방치하여 건조시켰다. 건조 후 점적한 평판 배지를 37℃에서 하루 정치 배양한 다음 여과액이 떨어진 위치에 투명환(clear zone)이 생성되는가를 조사하였다. 투명환이 생성되는 여과액의 경우가 장침입성 대장균을 사멸 시킬 수 있는 박테리오파지가 포함되어 있다고 판단할 수 있다. 이러한 조사를 통하여 장침입성 대장균에 대한 사멸능을 가진 박테리오파지를 포함한 여과액을 확보할 수 있었다.
장침입성 대장균에 대한 사멸능을 가진 박테리오파지의 존재가 확인된 여과액을 이용하여 순수 박테리오파지의 분리를 실시하였다. 순수 박테리오파지의 분리에는 통상의 용균반 분석(plaque assay)을 이용하였다. 이를 자세히 설명하면, 용균반 분석에서 형성된 용균반 하나를 멸균된 팁을 이용하여 회수한 다음 이를 장침입성 대장균 배양액에 첨가해 주어 4-5 시간 동안 함께 배양하였다. 배양 후 8,000 rpm에서 20분간 원심분리하여 상등액을 얻었다. 얻어진 상등액에 50분의 1의 부피로 장침입성 대장균 배양액을 첨가해 준 다음 다시 4-5 시간 배양해 주었다. 박테리오파지의 수를 증가시키기 위하여 이러한 과정을 최소 5회 이상 실시한 다음 최종적으로 8,000 rpm에서 20분간 원심분리하여 상등액을 얻었다. 얻어진 상등액을 사용하여 다시 용균반 분석을 실시하였다. 통상 순수 박테리오파지의 분리가 상기 과정의 1회로 달성되지 않기 때문에 이때 형성된 용균반을 이용하여 앞 단계를 전체적으로 다시 반복하였다. 이와 같은 과정을 최소 5회 이상 실시하여 순수한 박테리오파지를 포함한 용액을 확보하였다. 통상적으로 순수 박테리오파지의 분리는 형성된 용균반의 크기 및 모양이 모두 유사하게 될 때까지 반복하였다. 그리고 최종적으로는 전자현미경을 통하여 박테리오파지의 순수 분리 여부를 확인하였다. 전자현미경 분석에서 순수 분리가 확인될 때까지 앞에 기술한 과정을 반복하였다. 전자현미경 분석은 통상의 방법에 따라 실시하였다. 이를 간단히 설명하면 다음과 같다. 순수한 박테리오파지를 포함한 용액을 구리 격자(copper grid)에 묻히고 2% 유라닐 아세테이트(Uranyl acetate)로 역염색법(negative staining)과 건조를 수행한 후 투과전자현미경을 통하여 그 형태를 촬영하였다. 순수 분리한 박테리오파지의 전자현미경 사진이 도 1에 제시되어 있다. 형태적 특징으로 판단할 때 신규 확보된 박테리오파지는 미오비리대 박테리오파지에 속함을 확인할 수 있었다.
이런 방식으로 확인된 순수 박테리오파지를 포함한 용액은 다음의 정제 과정을 거쳤다. 순수 박테리오파지를 포함한 용액 전체 부피의 50분의 1의 부피로 장침입성 대장균 배양액을 첨가해 준 다음 다시 4-5 시간 배양하였다. 배양 후 8,000 rpm에서 20분간 원심분리하여 상등액을 얻었다. 충분한 수의 박테리오파지가 포함된 액을 얻기 위해 이러한 과정을 총 5회 반복하였다. 최종 원심분리로 얻어진 상등액을 0.45 μm의 필터를 이용하여 여과한 다음 통상의 폴리에틸렌 글리콜(Polyethylene Glycol; PEG) 침전 과정을 실시하였다. 구체적으로, 여과액 100 ml에 10% PEG 8000/0.5 M NaCl이 되게 PEG와 NaCl을 첨가한 다음 4℃에서 2-3시간 동안 정치한 후, 8,000 rpm에서 30분간 원심분리하여 박테리오파지 침전물을 얻었다. 이렇게 얻어진 박테리오파지 침전물을 완충액(buffer; 10 mM Tris-HCl, 10 mM MgSO4, 0.1% Gelatin, pH 8.0) 5 ㎖로 부유시켰다. 이를 박테리오파지 부유액 또는 박테리오파지 액이라 지칭한다.
이렇게 하여 정제된 순수 박테리오파지를 확보할 수 있었고, 이 박테리오파지를 박테리오파지 Esc-COP-4로 명명한 뒤, 2014년 8월 21일자로 한국생명공학연구원 생물자원센터(수탁번호 KCTC 12663BP)에 기탁하였다.
실시예 2: 박테리오파지 Esc-COP-4의 유전체 분리 및 서열 분석
박테리오파지 Esc-COP-4의 유전체를 다음과 같이 분리하였다. 유전체 분리에는 실시예 1에서와 같은 방법으로 얻어진 박테리오파지 부유액을 이용하였다. 먼저 부유액에 포함되어 있을 수 있는 장침입성 대장균의 DNA와 RNA를 제거하기 위해, 박테리오파지 부유액 10 ml에 DNase I과 RNase A를 각각 200 U씩 첨가한 다음 37℃에서 30분간 방치하였다. 30분 방치 후에 DNase I과 RNase A의 활성을 제거하기 위해, 0.5 M 에틸렌디아민테트라아세트산(ethylenediaminetetraacetic acid; EDTA) 500 μl를 첨가한 다음 다시 10분간 정치시켰다. 그리고 이를 추가로 10분간 65℃에 정치시킨 다음 박테리오파지 외벽을 와해시키기 위해 proteinase K (20 ㎎/ml) 100 μl를 첨가한 후 37℃에서 20분간 반응시켰다. 그 후 10% 도데실 황산 나트륨염(sodium dodecyl sulfate; SDS) 500 μl를 첨가한 다음 다시 65℃에서 1시간 동안 반응시켰다. 1 시간 반응 후, 이 반응액에 25:24:1의 구성비를 갖는 페놀(phenol) : 클로로포름(chloroform) : 이소아밀알코올(isoamylalcohol)의 혼합액 10 ml를 첨가해 준 후 잘 섞어 주었다. 그리고는 이것을 13,000 rpm에서 15분간 원심분리하여 층이 분리되게 한 다음 분리된 층들 중에서 위층을 취하여 여기에 1.5 부피비의 아이소프로판 알코올(isopropyl alcohol)을 첨가한 다음 13,000 rpm에서 10분간 원심분리하여 유전체를 침전시켰다. 침전물을 회수한 후 침전물에 70% 에탄올(ethanol)을 첨가한 다음 다시 13,000 rpm에서 10분간 원심분리하여 침전물의 세척을 실시하였다. 세척된 침전물을 회수하고 진공 건조 시킨 다음 100 μl의 물에 녹였다. 상기 과정을 반복하여 박테리오파지 Esc-COP-4의 유전체를 다량 확보하였다.
이렇게 얻어진 유전체를 이용하여 서울대학교 농생명과학공동기기원(National Instrumentation Center for Environmental Management)에서 illumina Mi-Seq 기기를 이용하여 차세대염기서열 분석(Next generation sequencing analysis)을 통하여 박테리오파지 Esc-COP-4의 유전체 서열분석을 실시하였다. 최종적으로 분석된 박테리오파지 Esc-COP-4 유전체는 168,161 bp의 크기를 가지며, 전체 유전체 서열은 서열번호 1로 제시되어 있다.
확보된 박테리오파지 Esc-COP-4의 유전체 서열을 기반으로 Web상의 BLAST (http://www.ncbi.nlm.nih.gov/BLAST/)를 이용하여 기존 알려진 박테리오파지 유전자 서열과의 상동성(similarity)을 조사해 보았다. BLAST 조사 결과, 박테리오파지 Esc-COP-4의 유전체 서열은 시겔라(Shigella) 박테리오파지 pSs-1의 서열(GenBank Accession No. KM501444.1), 대장균 박테리오파지 RB10의 서열 (GenBank Accession No. KM606999.1), 대장균 박테리오파지 RB9의 서열 (GenBank Accession No. KM606998.1), 대장균 박테리오파지 RB7의 서열 (GenBank Accession No. KM606997.1), 및 대장균 박테리오파지 RB6의 서열 (GenBank Accession No. KM606996.1)과 높은 상동성을 가졌다 (98%, 95%, 95%, 95%, 및 95%). 그러나 박테리오파지 Esc-COP-4의 유전체 서열은 전체 유전체 크기가 168,161 bp임에 비하여, 시겔라 박테리오파지 pSs-1의 전체 유전체 크기는 164,999 bp로 달랐으며, 대장균 박테리오파지 RB10의 전체 유전체 크기는 168,401 bp로 달랐으며, 대장균 박테리오파지 RB9의 전체 유전체 크기는 168,395 bp로 달랐으며, 대장균 박테리오파지 RB7의 전체 유전체 크기는 168,395 bp로 달랐으며, 또한 대장균 박테리오파지 RB6는 전체 유전체 크기가 168,394 bp로 달랐다. 이에 더하여 박테리오파지 Esc-COP-4의 유전체 상의 개방형해독틀(Open Reading Frame, ORF)의 개수가 267개임에 반하여 시겔라 박테리오파지 pSs-1의 유전체 상의 개방형해독틀의 개수가 266개로 달랐으며, 대장균 박테리오파지인 RB10의 유전체 상의 개방형해독틀의 개수가 272개로 달랐으며, 대장균 박테리오파지인 RB9의 유전체 상의 개방형해독틀의 개수가 272개로 달랐으며, 또한 대장균 박테리오파지인 RB7의 유전체 상의 개방형해독틀의 개수가 272개로 달랐으며, 또한 대장균 박테리오파지인 RB6의 유전체 상의 개방형해독틀의 개수는 271개로 달랐다.
이러한 결과로부터 박테리오파지 Esc-COP-4는 기존 보고된 박테리오파지들과는 다른 신규한 박테리오파지라 결론지을 수 있었다.
실시예 3: 박테리오파지 Esc-COP-4의 장침입성 대장균에 대한 사멸능 조사
분리된 박테리오파지 Esc-COP-4의 장침입성 대장균에 대한 사멸능을 조사하였다. 사멸능 조사에는 실시예 1에서와 같은 방법으로 점적 실험을 통하여 투명환 생성 여부를 조사하였다. 사멸능 조사에 사용되어진 장침입성 대장균들은 본 발명자들에 분리되어 장침입성 대장균으로 동정된 것들로 총 10종이었다. 박테리오파지 Esc-COP-4는 실험에 대상이 된 장침입성 대장균 중 9종에 대하여 사멸능을 갖고 있었다. 대표적 실험 결과가 도 2에 제시되어 있다. 한편, 박테리오파지 Esc-COP-4의 스타필로코쿠스 아우레우스(Staphylococcus aureus), 엔테로코쿠스 패칼리스(Enterococcus faecalis), 엔테로코쿠스 패슘(Enterococcus faecium), 락토바실러스 플랜타룸(Lactobacillus plantarum), 스트렙토코쿠스 우베리스(Streptococcus uberis) 및 슈도모나스 애루기노사(Pseudomonas aeruginosa)에 대한 사멸능 조사도 실시하였는데, 결과로 박테리오파지 Esc-COP-4는 이들 균종들에 대해서는 사멸능을 갖고 있지 않았다.
이상의 결과로 박테리오파지 Esc-COP-4는 장침입성 대장균에 대하여 특이적인 사멸능을 가지며, 다수의 장침입성 대장균에 대하여 항균 효과를 발휘할 수 있음을 확인할 수 있었다. 이는 박테리오파지 Esc-COP-4가 장침입성 대장균의 감염 방지 및 처치 목적의 조성물의 유효성분으로 활용 가능함을 의미한다.
실시예 4: 박테리오파지 Esc-COP-4의 장침입성 대장균의 감염 예방에 대한 실험예
9 ml의 TSB 배지를 담은 하나의 튜브에 3× 108 pfu/ml 수준의 박테리오파지 Esc-COP-4 액 100 μl를 넣어주고, 다른 하나의 9 ml의 TSB 배지를 담은 튜브에는 동량의 TSB 배지만을 추가로 첨가하였다. 그 다음에 각 튜브에 600 nm에서 흡광도가 0.5 정도가 되도록 장침입성 대장균의 배양액을 넣어 주었다. 장침입성 대장균을 첨가한 후 튜브들을 37℃의 배양기에 옮겨 진탕배양 하면서 장침입성 대장균의 성장 상태를 관찰하였다. 표 1에 제시된 바와 같이, 박테리오파지 Esc-COP-4 액을 첨가해 준 튜브에서는 장침입성 대장균의 성장 억제가 관찰된 반면에 박테리오파지 액을 첨가하지 않은 튜브에서는 장침입성 대장균의 성장 억제가 관찰되지 않았다.
장침입성 대장균의 성장 억제
구분 OD600 흡광도 값
배양 0분 배양후 60분 배양후 120분
박테리오파지 액 미첨가 0.5 1.6 2.0
박테리오파지 액 첨가 0.5 0.4 0.3
이 결과로부터 본 발명의 박테리오파지 Esc-COP-4가 장침입성 대장균의 성장을 저해할 뿐만 아니라 사멸까지 시키는 능력이 있음을 확인할 수 있었고, 이로부터 박테리오파지 Esc-COP-4가 장침입성 대장균의 감염을 방지하는 목적의 조성물의 유효성분으로 활용될 수 있다고 결론지을 수 있었다.
실시예 5: 박테리오파지 Esc-COP-4를 이용한 장칩입성 대장균의 감염 질환 처치예
장침입성 대장균에 의한 질환이 유발된 동물에서의 박테리오파지 Esc-COP-4의 처치 효과를 조사하였다. 생후 25일령의 이유자돈 4마리를 한 그룹으로 하여 총 두 그룹으로 나눈 후 실험사육돈방(1.1m × 1.0m)에서 분리 사육하면서 14일간 실험을 실시하였다. 보온시설 하에 주위환경을 통제하였고 돈방의 온도와 습도는 일정하게 유지시켰으며 돈방 바닥의 청소를 매일 실시하였다. 실험 개시일로부터 7일째 되는 날에 모든 돼지들에게 장침입성 대장균 액을 경구 주입관을 사용하여 1 ml 경구투여 하였다. 투여한 장침입성 대장균 액은 다음과 같이 준비한 것이다. 장침입성 대장균을 TSB 배지를 이용하여 37℃에서 18시간 배양한 후 균체만을 회수한 다음에 이를 생리식염수 (pH 7.2)로 109 CFU/ml가 되게끔 조정하였다. 장침입성 대장균 투여 다음날부터 실험군(박테리오파지 액 투여군)의 돼지들에게는 매일 2회씩 109 PFU의 박테리오파지 Esc-COP-4를 장침입성 대장균 액 투여와 같은 방식으로 경구투여 하였다. 대조군(박테리오파지 액의 미 투여군)의 돼지들은 어떠한 처치도 하지 않았다. 사료와 음수는 대조군과 실험군 모두 동일하게 급이하였다. 장침입성 대장균 투여 후부터 매일 모든 시험동물들을 대상으로 설사 발생 상태를 조사하였다. 설사 발생 상태 조사는 설사지수를 측정 방식으로 실시하였다. 설사지수 측정은 통상 사용되는 Fecal Consistency (FC) score (정상: 0, 연변: 1, 묽은 설사: 2, 심한 설사: 3)를 측정하는 방식으로 실시하였다. 그 결과는 표 2와 같았다.
설사지수 측정 결과
장침입성 대장균 투여 후 경과 일 0 1 2 3 4 5 6 7
대조군 (박테리오파지 액 미투여) 2.5 2.25 2 2.25 2 2 1.5 1.5
실험군 (박테리오파지 액 투여) 2.5 1 0.5 0.25 0 0 0 0
이 결과로부터 본 발명의 박테리오파지 Esc-COP-4가 장침입성 대장균을 원인으로 하는 감염 질환의 처치에도 매우 효과적이라는 것을 확인할 수 있었다.
실시예 6: 사료첨가제 및 사료의 제조
박테리오파지 Esc-COP-4 액을 이용하여 사료첨가제 1 g당 1× 108 pfu의 박테리오파지 Esc-COP-4가 포함되도록 사료첨가제를 제조하였다. 사료첨가제의 제조 방법은 박테리오파지 액에 말토덱스트린을 첨가 (40%, w/v)하고 여기에 전체 10%가 되게 트리할로오즈를 첨가한 다음에 동결건조시켜 제조하였다. 최종적으로 고운 가루 형태로 분쇄하였다. 상기 제조 과정 중의 건조 과정에는 감압 건조, 가온 건조, 상온 건조도 대체 가능하다. 대조 실험을 위해, 박테리오파지가 포함되지 않은 사료첨가제도 박테리오파지 액 대신에 박테리오파지 액의 제조 시에 사용한 완충액(buffer; 10 mM Tris-HCl, 10 mM MgSO4, 0.1% Gelatin, pH 8.0)을 사용하는 방식으로 제조하였다.
이렇게 제조된 2종의 사료첨가제 각각을 중량비로 1,000배의 양돈용 사료와 혼합하여 최종 2종의 사료를 제조하였다.
실시예 7: 음수첨가제 및 소독제의 제조
음수첨가제나 소독제는 그 활용에서만 차이가 나고 제형은 동일하므로 같은 방식으로 제조하였다. 박테리오파지 Esc-COP-4 액을 이용하여 음수첨가제 (또는 소독제) 1 ml당 1× 108 pfu의 박테리오파지 Esc-COP-4가 포함되도록 음수첨가제 (또는 소독제)를 제조하였다. 음수첨가제 (또는 소독제)의 제조 방법은 박테리오파지 액 제조 시에 사용하는 완충액 1 ml당 1× 108 pfu의 박테리오파지 Esc-COP-4가 포함되도록 상기 박테리오파지 Esc-COP-4 액을 첨가하여 잘 혼합해 주는 방식으로 제조하였다. 대조 실험을 위해, 박테리오파지가 포함되지 않은 음수첨가제 (또는 소독제)로는 박테리오파지 액의 제조 시에 사용한 완충액 자체를 그대로 사용하였다.
이렇게 제조된 2종의 음수첨가제 (또는 소독제)는 부피비로 1,000배의 물로 희석하여 최종적인 음수 또는 소독제로 사용하였다.
실시예 8: 돼지 사육에서의 사양 효과 확인
실시예 6 및 실시예 7에서 제조한 사료, 음수 및 소독제를 이용하여 돼지 사육 시의 사양 결과 개선 여부에 대하여 조사해 보았다. 특히 본 조사는 실시예 5에서 사용한 설사지수 기반의 설사 발생 상태 조사 방식으로 실시되었다. 총 30 마리의 자돈을 10 마리씩 한 그룹으로 총 3개 그룹(사료로 급이한 그룹-A; 음수로 공급한 그룹-B; 소독 처리한 그룹-C)으로 나누어 2주간 시험을 실시하였다. 각 그룹은 다시 5마리로 구성되는 소그룹으로 나누어지며 각 소그룹은 박테리오파지 Esc-COP-4가 적용된 소그룹 (소그룹-①) 및 박테리오파지가 적용되지 않은 소그룹 (소그룹-②)으로 나누었다. 본 시험에 대상이 된 자돈은 20일령의 이유 자돈이었으며, 각 시험 소그룹의 자돈은 일정 간격을 두고 위치한 격리된 각각의 분방에서 사육되었다. 각 소그룹은 다음의 표 3과 같이 구분되고 지칭되었다.
돼지 사양 시험에서의 소그룹 구분 및 표시
적용 소그룹 구분 및 표시
박테리오파지 Esc-COP-4 적용 박테리오파지가 적용되지 않음
사료로 급이한 그룹 A- A-
음수로 공급한 그룹 B- B-
소독 처리한 그룹 C- C-
사료 급이의 경우에는 실시예 6에서 제조한 사료를 표 3의 구분에 따라 통상적인 사료 급이 방식을 따라 급이하였으며, 음수 급이의 경우에는 실시예 7에서 제조한 음수를 표 3의 구분에 따라 통상적인 음수 급이 방식에 따라 급이하였으며, 소독 처리의 경우에는 일주일에 3회씩 기존 소독과 번갈아 가면서 실시하였다. 본 발명의 소독제를 분무하는 날은 통상의 소독제를 이용한 소독은 실시하지 않았다. 시험 결과가 표 4에 제시되어 있다.
돼지 사양 시험에서의 설사지수
그룹 설사지수
d1 d2 d3 d4 d5 d6 d7 d8 d9 d10 d11 d12 d13 d14
A- 0.2 0 0 0.2 0 0 0.2 0 0.2 0 0 0.2 0 0
A- 0.2 0.4 0.4 0.4 0.2 0.2 0.4 0.2 0.4 0.4 0.4 0.4 0.2 0.2
B- 0 0 0 0 0 0 0.2 0 0 0 0 0 0.2 0
B- 0.2 0.4 0.2 0.4 0.4 0.4 0.2 0.4 0.4 0.4 0.2 0.4 0.4 0.2
C- 0.2 0 0 0.2 0 0.2 0 0.2 0 0 0 0 0 0
C- 0 0.2 0.4 0.2 0.4 0.4 0.4 0.4 0.4 0.2 0.2 0.4 0.2 0.4
이상의 결과로 본 발명에 따라 제조된 사료 및 음수의 급이와 본 발명에 따른 소독 처리가 동물 사육에서의 설사증 감소에 효과가 있음을 확인할 수 있었다. 이로부터 본 발명의 조성물의 적용이 동물의 사양 결과 개선에 효과적이라는 결론을 내릴 수 있었다.
이상으로 본 발명의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현 예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항과 그의 등가물에 의하여 정의된다고 할 것이다.
한국생명공학연구원 KCTC12663BP 20140821
SEQUENCE LISTING <110> iNtRON Biotechnology, Co. Ltd. <120> Novel enteroinvasive Escherichia coli bacteriophage Esc-COP-4 and its use for preventing proliferation of enteroinvasive Escherichia <130> KP090376 <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 168161 <212> DNA <213> Bacteriophage Esc-COP-4 <400> 1 agtatgaata taattaataa gatttttgga attcaataca ttaaagtcac atataaagta 60 acgtataaag gtccgtatac cgatgaacat gaagaaccac aagttaagtc tattatatta 120 gaaaaggacc ctaactggcc agttgaattt cgtcttcctt gctatagtca ttgggctgat 180 gttgaaatta taagtattga aaatgtctga gttagagatt agaagcaatt ttagatggcc 240 atcatgcgca ttaagtaatt tcgcccaatg gccttttgtt atggatagta tccaatttgg 300 aggtctcgaa ggattcctcc aagggtgtaa ggtgaaaaat gttgaacagc aacgtcgtat 360 atttgggtta tccgggcttg ccgcccaaca agctggacga gcttatgcta gagctcagga 420 ccgtggcacg ttgttctggc ttggagttcc gttttcaaga tactctccag cgtggaaaga 480 attatacaca aatgcatatt ttgaagcagc gttccaaaat aagggctttc gtgatgcatt 540 acaagcctcg aaaggaaaag ttttgaagca cagcatggct agtggtctaa caaaagatga 600 tacaatacta accgaagctg aatttattga tgtgttaaac ctattaagag actctctatg 660 aagcctacta ttttaactga tattgatgga gtatgtttaa gctggcaatc aggacttcct 720 tattttgctc agaaatataa tcttccgtta gaacatattt taaaaatgat ccaagatgag 780 aaatttattt ctccaggtaa actctttaat tgtgatgaag aacttggtgt caagttaatt 840 gaaaaataca atcgttcaga ttttattcgt tacttgtctc catataaaga tgcattgtgc 900 gtaattaaca aactaaaaga agattataat tttgtggctg ttacggcatt gggtgattct 960 attgacgctc tgctgaatcg tcaatttaat ttgaatgctc tttttcctgg tgccttctca 1020 gaagtaatga tgtgtggtca tgattcttca aaagaagagt tattcaaaaa ggcaaaagag 1080 aaatataacg taatttgtta tattgacgat ctcgctcacc attgcgatca tgcgagtgaa 1140 atattaaatg ttccggttta ttggatggct cgaggagagc gtgacaatat tccaaaaact 1200 gctcagcgag tttatacatg gaatgatgta gaaaataagc ttttttcgcc gaaggaaaat 1260 aaagaaagtt ttgatagtga aaaagctata aaagatttaa ttgagaagat ggttaaaaac 1320 gattcttttc cttggaacac tacctggaga acgcctggat ttaatcctta taatcatcta 1380 tatcatccat atcatccata tcagacacat ccatttcaga catggaacta tattaaacct 1440 ggtggcatag agtatttgta taatagacct acttgtggtg ataatatttt ccaaggagca 1500 ttctaatgtt tgtcgttcac actatttatg aaaatgaagg taatattaca cgtgattatg 1560 gtcacgtaaa tcaatttttt agatgcaatc cagaattccg tgctcaaaaa gacgaacgaa 1620 tttttaaaaa atgtgtagaa caaggtttca tttacgtcaa gcactggatg caaggaaata 1680 aagttagaac tacatatcat aagtctttgc ctgagcttaa tgatgaattg atttataata 1740 gagatgtaaa tcaaactctg aaggatgaac aatgattctt aaaattctga atgaaatagc 1800 atctattgga tcaactaagc agaagcaagc aattcttgaa aagaataaag ataacgaatt 1860 gcttaaacga gtatatcgtc tgacttattc tcgtgggtta cagtattata tcaagaaatg 1920 gcctaagcct ggtattgcta cccagagttt tggaatgctg actattaccg atatgcttga 1980 cttcattgaa ttcacgttag ctactcggaa attaactggc aatgcagcaa ttgaggaatt 2040 aactggatat atcaccgatg gtaaaaagga tgatgttgaa gttttgcgtc gagttatgat 2100 gcgagacctt gaatgcggtg cttcagtatc tattgcaaac aaagtttggc cgggtttaat 2160 tcctgaacaa cctcaaatgc tggcaagttc ttatgatgaa aaaggcatta gtaagaatat 2220 caaatttcca gcctttgcac agttaaaagc agatggagct cggtgttttg ctgaagtccg 2280 aggtgatgaa ttagatgatg ttcgtctttt atcacgagct ggtaatgaat atctaggatt 2340 agatcttctt aaggaagaat taatcaaaat gactgctgaa gcccgccaga ttcatccaga 2400 aggcgtgtta atcgatggcg aattggtata ccatgagcaa gttgaaaaag agccagaagg 2460 cctagatttt ctttttgatg ctcctgaaat cagtaaagct aaagagtttg ccgaagtagc 2520 tgaatctcgt actacttcta atggaatcgc caataaatcc ttaaaaggaa ccatttctga 2580 aaaagaagct caatgtatga agtttcaggt ctgggattat gtcccgttgg tagaaatata 2640 cggtcttccg acgtttcgtt tgaagtatga tgcacgtttt tctaaactag aacaaatgac 2700 atcaggatat gataaagtaa ttttaattga aaaccaggta gtaaataacc tagatgaagc 2760 taaggtaatt tataaaaagt atattgacca aggtcttgaa ggtattattc tcaaaaatat 2820 cgatggatta tgggaaaatg ctcgttcaaa aaatctctat aaatttaaag aagtaattga 2880 tgttgattta aaaattgtag gaatttatcc tcaccgtaaa gaccctacta aagcaggtgg 2940 atttattctt gaatcagagt gtggaaaaat taaagtaaat gctggttcag gcttaaaaga 3000 taaagccggt gtaaaatcgc atgaacttga ccgtactcgc attatggaaa accaaaatta 3060 ttatattgga aaaattctag aatgcgaatg taatggttgg ttaaaatctg acggccgcac 3120 tgattacgtt aaattatttc ttccaattgc gattcgttta cgtgaagata aaactaaagc 3180 taatacattc gaggatgtat ttggtgattt tcatgaggta actggtctat gaaagcttac 3240 ttagaaacaa ttgtcgttgc tcaaaaagaa ggtggagatg tttctacttc tgtatcacaa 3300 attatgattg aatttataga tgcgtatgct tataataaat tcacagaaac atttgatgcc 3360 tatgaaaaag gtccaaagtt tgaaatatac cgtactctct taccaataga ttattaaagg 3420 ccttcgggcc tttaatttta taaatagaat aaacactaga gaggatatga tggaactcat 3480 tacagaatta tttgacgaag atactactct tccgattaca aacttaaatc caaagaagaa 3540 aataccgcaa attttttcag ttcatgttga tgacgcaatt gaacaaccag gctttcgttt 3600 atgtacctat acatctggag gtgatactaa tcgcgattta aagatgggcg ataaaatgat 3660 gcatattgtt ccttttacat taactgctaa aggttcaatt gctaaattaa aaggtctcgg 3720 tccaagccca attaattaca tcaattcagt ttttactgtt gcgatgcaga cgatgcgtca 3780 gtataaaatt gatgcctgca tgctccgtat tcttaagtct aaaactgctg gtcaagctcg 3840 tcaaattcaa cttattgctg atagacttat ccgtagtcgt tcaggtggca gatacgtcct 3900 tcttaaggaa atctgggatt acgataaaaa gtatgcatat attatggttt accgtaaaaa 3960 tgccaattta gaagacattc caggtgtacc tcctatttca actgagttat tcacaaaagt 4020 tgaatcaaag gttggtgatg tttatgtaga tgttaaaacg ggtaatgctg ttcctaaagc 4080 tgttgctgtt gctgcttcta ttgctttaga aaatgataag cgcacggatc aagctgttat 4140 tcagaaaact aaaattagtc gtcgattagc agcgcaagct caatattcta ctgttgatgc 4200 ttcacttcag ggtgatagct tcgccgctaa gaaatatcaa gagtttgaat ctaaagttcc 4260 ggtatataaa gcagaaggac caatgaactc tggcgttatt cagattggtt caaacttcgg 4320 caaaggagct atcggtggta tgagaagtgc ttctcgtttt aaatctaacg attatgaact 4380 agaaagcttc cgaaatcata ttgcattggc ccatgcacgt ttacgtgatc cgtctatcag 4440 actgcagagc gatataacac atcaaggttc tcaagaatat ttaaagaata aagaattctt 4500 tgattataaa accgataaaa ttttgagcga tctcgctgac attaatattt ctaatagctt 4560 tgatgttatt aagaaaatta tcaatgattt agttaaaggt tctaaagcta cgccagacga 4620 aaaggtagct attattcaat ttgtcatgaa tggcatttat aaattgatta atgaatctgc 4680 tgctcaggca tatgaatacg caagcaccga agtaactcca aaaggactga ctcaggctga 4740 gtctgatgta attgaagatt attgtgcaga ttcatatgtt gaaatgaact cgttcctttt 4800 aggtaaacca gattctaccc gtgaagaata catggaacga gctattaaac acatcgagac 4860 tctggattct gcattcgcca aaggatcagt tcttcctccg ggaactacgc tttatcgtgg 4920 tcaagaagtt acttttaaaa ctttacgcca taatattgaa aataaaatgt tctatttcaa 4980 gaacttcgta tccacatcgc ttaaaccaaa tatcttcggt gagcatggta aaaactatat 5040 ggctctagat gattccggcg cagtattttc tggtgaagga gaaggttctg ttgatgctga 5100 agatttaatg catatgggca gtcattctgc ttatgcgaat gaagatgctg aaactagcgt 5160 aggtatggta attaaagggg ctgagcgaat caaagttatc gttccaggtc atttatcagg 5220 atttccatca gaagctgaag ttattctacc gcgtggaatt ttgttgaaga ttaataaagt 5280 aagcacgtac atgatgaaag aaactgctta taacaagtat ctaatcgaag gcacaatcgt 5340 tcctccttct gaacaattag aagaatcggt atatgatggc gatcatctaa tggaaactgg 5400 tgaagttcgt ccaatggctg ggtttaatca attccttgta gaagaatcaa aagaagagga 5460 aaacgaagtt tctcaaatct tggcttcttt ggttaacatc aacagaatgt ctaaaaagtt 5520 caagatgtag tttacaagtc cctcgtgttg tgttatagta gtcttactga cataacatga 5580 ggaacacaaa atgaaatctt ctttacgctt tttaggtcaa gaacttgtag ttgaaggcgt 5640 tattcctgct gataacgctt ttaatgaagc agtttatgat gaatttatca aaatttttgg 5700 aacagataaa aagttcggaa tttttccttc tgaaaatttt tcaaagccag aacagactga 5760 aagcattttc caaggtgtag taacaggtaa atttgagtca gaagctccgg taaaaattga 5820 agtttatatt gaagacagtt tagttgcttc agttgctgct ttcatttcat tccgtaaata 5880 aaaatatggg gacctttcgg tccccattgt tatattgctc ctaatatttt actctgcgag 5940 ttgacaattc ccgtcatagt attaatgttt gaaatgcttc cagcagttcc ccctaatcta 6000 ctaagtcgtg aaagcgaatt agataatcca gtaacgcctc cactatttcc gagaacgctt 6060 tgaataccgt ttatagcagc agattcaagc cattcaaggg cagcttgtct atcaactgct 6120 ccagcctgca tgactctata cgcaaaagta acatcaaacg tagttatttg gttatctcca 6180 tcatatgata actcaggagc gctcactgac actggaatgc atccggtgaa catcactgca 6240 gtatgaggta atccattacg agcatgaaga ttaacctgaa tatctgcctc gacatcttgt 6300 ggcaaagcac gcagtccagt tactgggtct tgaacagcgt taacccagtc ttgcattgca 6360 cgatagttac aagcttctga atccattcta aatgaaataa ccaaagggtc taattctctc 6420 ccagttatac gaatattagg agagttatag ttccagtcag tttcatagga taatctattc 6480 tctggcattt ttacagaata tatcatcaac ccagatgagt tatatgccat gttaaagaag 6540 tcaattaaat atgtaccaac tgtaaatgag cccaataaac tttgaactgt acgttgactc 6600 atggcaccaa taagatattt actaacccct gattttctta tcagtttttg tgtgccagca 6660 gtaattagcg tggtaattcc ctgattaata tcgccttgag acaatcctaa ccagtctgaa 6720 tttagaccca aattattata agaaaagttg ctaattgaac ttatcaacga agagctttta 6780 gttgatggag ttgtcgcaaa aacgcagcta aacatattat tacgttggaa atctgcgttt 6840 attgcttgat tattaaattc ctctaaagaa tacattaaaa agtccccgca tataaagaag 6900 cacgatttaa cgtgataatt tctctcatag taatctcaag agtaaatgta ctagggaggt 6960 ttggagcaat agctaatccg ttaaagttac cattaggtgt tttatcaaat ctgatgctct 7020 gtatttggca tgggccaaat atttccgttt ttccatcaaa cttagatgtt gcaccaaagt 7080 ttttcaccat ccaaattgtt gggtttgaaa ctactagaac gttagttaaa ctcgatgtca 7140 ttttctcaaa tagtgtttta tttttagctg cgtcttccgg agataaaggt tcaattaatg 7200 tagaacgata ccaatcatct aaatacccct ttatttcagc agcatattga gatttgcccg 7260 tttcaccata agaaaaatag ttaaaatatt gatagatatt gataatagcc atcaaatctt 7320 ctgttgaacg cggagtcaaa tcccatgtaa acactttagt tctattctca gcaccaccat 7380 acatacttct ggctgtcgta taaatctgtt cattattatc agccattata ccttgtgtta 7440 tactttccag tgccccaaat actgcagttg aagcaatatt acttaacaca ccagtagcag 7500 tacctccgcc tctgctgata agactttctt gaacatcatt aaatctatgt gatgatgtat 7560 caacgtcaga tttagatctc ggtaaaagaa tgtttgcgac aggagcttta cttattgttc 7620 ctgaattatt gtttgatatt aatccatttg atagttttga tactgtacta ctaatagtgt 7680 ttctagctgt acttaaaata ctcgaagatg aagaagagta gttagatctc atcgatctaa 7740 gacttccaga atccctagat gacatattgt atgcagtaaa taataatcca ttcttatata 7800 ggtctgttac ctggaagtcc cctgtagtgt cattaccact agcacgccca gttggaaact 7860 gggcagtgta tgttttagtt gctacttctg atttagtact ctgtccggct gaaattttct 7920 caccggactt tttaattaaa tcagcagtta tttctttaac aattgccata ttattcctta 7980 attaactcca gtcgcatcaa atacaccagg agcagtcgtg ctcgttacgg gtgtcatatt 8040 atgaacgaca gtatttttct taataacatt attagtatta ttgattgaag gagatgcttg 8100 ttgaacagga gcttgctgtg ctttgttctt ttctattacc tggacttgtt ttgcttctgg 8160 cgatttagca gaagtttgag gtttggcatt aggctgattt ttcttgagct cttgataagt 8220 agcatcaatt ttagaaaatc tagcagcaag ttctttttta actgccggtg aattatttaa 8280 atccgggtca tccatccgtt ttttaaggtc ttcataggca gcttcaactg atttaaccgt 8340 tgagtcttta ctcatatcag ctgaattagc gtattcatca aaacgattca tagcagcacg 8400 agcttcatta gctttcatta aagcaatttt agcttcctcc ggagaaagtt gctttaattt 8460 ttcttcttcc tctgctctct cttcatcagt cgttagtgct tctttattat caacaccacg 8520 aatccagtta gatgcacgag ttttccagtt cgcaattttg tctagccctt ttgctattgg 8580 accaagatcg ccattcattc ttttatattg ataatttgca actttttctt ggtcttcttt 8640 gctaagagaa gcatttgtag tattttggaa attttctagt gctcttcctt ctacttcgtc 8700 agcagtgtct ttcatgccag gaataactcg aagaattgcc gcagataatt tagccattcc 8760 aagttgaata agctctccta aattaaaaag aacctttcca agcccttcta caatagctgc 8820 tgtcaatccg ccccaatctc cggcttccca cagcttctta attttatcaa tagaactaaa 8880 gatgctctgt aataaaggac cccatgttcc ggtttcgcta gagaatttag taaagtttga 8940 actaaacaaa tcccaagcct gcgaaaattt atctgaccaa tatttaaagt gaaccatcag 9000 cagatctatt ccaacaacaa cagccaatat cattgcagtc attttagcgg cttcaatagc 9060 agcactgacg gtatacttaa atagcatgct tgatatttta tcagtaattg aaatagattt 9120 cttaaatccg aaatcaacag tctttgttaa tttatctaaa gcttgagata attttaagtt 9180 aaatgcgtct tttttctgtt tttcttctgg cgattcttgt ttgggttcaa ctggctgagg 9240 ggtagggaaa aaatcagcat caggatcatt attaaccgct tcaggagctg gtaataaagg 9300 accaacagat tcagctgtat catcctcaac gactttaaca ggaatagcgc tttcaaccgt 9360 ggctaaacta gttcctgttt gttgaattcc ggctgtctga attttttgct ctagtaaact 9420 cgttaattta tctaatttac ttccgagcga ttcgccaatt tctttattaa tattgttgcc 9480 aatttcgaca gtttcagcaa ttaactcaga accagcagta gtatcactca ctgcgctttc 9540 tacattgtca attgctccaa ttatttcatt cgatttttct tcaacggttt gagctattaa 9600 ttcagaagca gcttgagcat catccaattt tgtagaaatg tcattaagcc cagataaagt 9660 gttagaagcg gatttagccg cttcctgaac tggtttatta tctgaaataa cttttctacg 9720 catcgtttgc atttcttgtg gctttttcat tcaaataatc caataatatt gccaattcca 9780 gttattggac cattagggcc aggaattgct aaagttgtaa aaatatcatt tgcccatttt 9840 aaaacgaatg ctggcatctc aagaaaatta atttctttaa cttcatcatt gaccttaagc 9900 aagcatttag ataacatatc gctcaccgtt aaaaattgtt caaattttcc aggaggtcta 9960 aaataaaatg tatttccttg gtattgaaat tctaatcttt ggcacacata aacatcatta 10020 atgtcataag tataaccatc tatttcttta cgagatttaa tctttccatt aaattccaat 10080 aaatgaatag aaacgaaatc aacttctgct ggtgataaat tcggacaaat agaatcaata 10140 agaagtttta aattttcatc aggaccttta acatctttta aaatgttata atgtttaaga 10200 cccattttag gaatagaaac ttctttattg cttattggaa gaactacttt cttcagtggt 10260 agtatcagat ttaaattcat ttttaacctt aactgggtct attgtttcca gtttcgttgc 10320 attagtgaac atatatagat gagttactga attattattg gataattcat ggataacttc 10380 atcaacgtaa aattctgttt taaattggtt tttactatca ttaaaaataa ttttaacgcc 10440 aggagtcaag ttaaaattac cgacagtaga acatttagca tagccatcgt attgtgccat 10500 agtttgaaga cgaatagctt cttcatatcc attcctataa gtcatttcag aataagcacc 10560 tgaccttgat actacaatag agttttcgcc ctttcctgta gtaatcattg gcaatgaaga 10620 atctaaaaat gaatgagcat agatagtagc atttttcatt gggtcgcgtt tatgaggatt 10680 cgatttagtc aaccaaacga aatcatatgc taatggatat tttaattctt ggatgaattg 10740 acctattaaa gatggctcac cgacaatcat tggatacggt tcttgattta tcatcatatc 10800 atagtccatc atgttaactc ccatgatgtc ttgccacaca aatacaaatt tgtcacttcc 10860 tacagctaga gcaacttccc tgacatacga caaatagttt tcaaatgtgc tagtccatgg 10920 aatatcagga acataagcat ttatagcatt tattgctgga gttaataacg tgcgatcctg 10980 ataaatgacg ccaagcattt cctttataga ttcacctgca tcagagaaaa acggtctgcc 11040 aaatttaaga ttttctatgg aatgaatagt tcccaattca atagcaatga tgttatcacc 11100 ttttgaatct acagatacag aaaaatgctt acatccataa attcgtgttt taacattatt 11160 aatatcgttt gcattagcta cagaaatctg aattatttca tttccatcca tttttgtatg 11220 gatattttta gaatcataaa actgcagcat tccttcattt cggccataaa gagaatcccg 11280 catagttaat gtggtaatag tagcagctaa ttcaacaaat ctattattac tccaagcgtc 11340 gtagctatca aataatttaa cgctgagatt tggatatcct gggcgttgca acatactcat 11400 tattgtttat ccttctcaat cagttttaat acgaatccgc gctcagcagg aatcattttc 11460 attattgaat ttaagctata attacttttt acgagcgtgt gattgatttg ataaaaagta 11520 aatatttcat ctgggttaac taatagctta aacacatcta ctatatcagt gtattttttt 11580 acatacttat tacaacatga catatgcaat gttaaattaa taggattcat tgcatcgaga 11640 attttttcta atgtttctat ctcgatggca tcaacaagtt ctatttgact cgattcgctg 11700 atttccttcc aatcatacca catttcatct acttgaacag aatgaatatt ttcagtaatc 11760 atctttgctt tattttcata aaactcagaa ggaaacttta atttaatttt aacattagct 11820 acatcaaaaa caggttcctt taattctttt tgatatattt caaatggaac tgtcttttct 11880 tttttacatt ttggacatac aaatgtgacc ggtactttag ttttacctat tgaccctaca 11940 aatacctgca aaaatataaa tggttgccaa gtcttcggat agtctccaaa ataatcagca 12000 attaaatcag caattatttc tttttgttct tgtggtgacc gatgttctat atcgtttcga 12060 actaacaaaa aatctcgata atcttctacc gtaaatggtt taaaacgatg aacaccatct 12120 ggtaatttac aacgaataat gtttgccata gatgctcctt ttattctatt tataaatatg 12180 ataaataaag gagctaaata tgtatgaata caaatttgat gcgagagttg gttctaaaat 12240 aatcaactgt cgcgcattta ctcttaaaga atatctagaa cttattactg ctaaaaagaa 12300 cggttcagta gaagagatcg ttaaaaagct aatcaaagaa tgcacaaatg caaaagattt 12360 aaaccgccaa gaatcagaac tactgctgat tcatttatgg gcacattctc ttggagaagt 12420 taatcacgaa aactcctgga agtgcacctg tggaactgaa ataccaaccc atataaatct 12480 attacataca caaatagatg caccagaaga cctctggtat acactgggtg acattaaaat 12540 taagttccat taccctaaaa tttttgatga taaaaatata gcccacatga tagtatcatg 12600 tatagaaacg attcatgcta acggggaaag cattccagtt gaagacttaa atgaaaaaga 12660 actggaagat ttatattcta tcatcacaga gtcagatatt gtagctataa aagatatgct 12720 tttaaagcct actgtttatt tggctgttcc aattaagtgt ccagagtgtg gaaaaactca 12780 cgctcatgta ataagaggac tcaaagaatt ctttgagcta ttgtaatggc aaatattaat 12840 aagctttatt ctgacattga cccagaaatg aaaatggatt gggacaaaga tgtttcaaga 12900 tctcttggat taaggtcaat taaaaatagc cttttaggaa ttattacaac caggaaaggg 12960 tcaaggccat ttgacccaga atttggatgt gatttatctg atcagctttt tgagaatatg 13020 actcctctta ctgctgatac tgttgagcgt aacattgaaa gcgcagtaag aaactacgag 13080 ccgcgtattg ataaattagc agttaatgta ataccggttt atgatgatta tactctgata 13140 gtagaaatac gcttttcagt catcgataac cctgatgata ttgagcagat aaaactacaa 13200 ctggcttcca gtaacagagt ataatgcttc acgtataaac gtggtataat gaatctaagt 13260 ccatccaata acaattgaat agagaacaat atgaaattag aagatcttca agaagaattg 13320 aagaaagatg tgtttataga ttcgactaaa ttacagtatg aagcagctaa taatgtgatg 13380 ttatatagta aatggctcaa taagcattca agtattaaaa aggaaatgct tagaattgaa 13440 gcacagaaaa aagttgctct taaagctaaa ttagactact actcgggacg aggagatggt 13500 gatgaattta gtatggaccg ttatgagaaa tcagaaatga agacagttct atcagctgat 13560 aaggatgttt taaaagttga tacttcattg cagtattggg gaattttatt ggatttctgt 13620 agcggagctc ttgatgctat taaatcacgc ggatttgcta ttaagcatat tcaagacatg 13680 cgagcatttg aggctggaaa ataatgagat atagcattga tgatgctttt aattatgaag 13740 aagaattcga aacggaaatt caatttttaa tgaaaaagta taatcttaag cgtcaggata 13800 ttcgtatcct ggccgaccat ccatgtggtg aagatgtact ttatgttaaa ggaaaatttg 13860 ccggatatct tgatgaatat ttttattcta aagatatggg cattgatatg catatgagag 13920 ttatataaat agatatataa tttagaggag acaatcatgt cagataagat ttgcgttgtc 13980 tgtaaaactc caatcgattc tgcattggtt gttgaaacag acaaaggtcc tgtacatcct 14040 gggccttgtt ataattacat taaagaacta ccagtttcag aaagttcgga agaacaatta 14100 aatgaaacac aacttttgct atagtgtgac ctttagtcta tagttttggc ccttcctttt 14160 tggttgggcc ttttttaatt taaaagcttt cttctacttc atcgtctgaa tcttctaatt 14220 cagctctttt tcctgccaaa gcatctctca cagagatgtc atcagtatct tttaattcag 14280 tttctttaac tcttttctta taataagctt caagttcttc tagaccttct attgtttgac 14340 aagaggcaat tttacccata aattcatcaa tagaagcttc ataaagaaat tgtttaaatt 14400 ctagtaacat ctttttctcc aaagggccga agcccttata aattaactgt ttttctaagt 14460 attcttagtt tgtacattga taatccagta gctttagaag catctttcat gcagttatac 14520 ttaacaccat ttacttctat aggtttagca gctgggttat tagctcctga attatctggc 14580 atgttatctt taattttctg aatagattta gcggaatgtt tccttccata catgccatta 14640 tttttacctg attgattgat agaatgcaat cttttagctt cttcagtcca ttttgttggg 14700 ttcattcttt tagttttact aattttactt ccccacgtaa ttggacgatt tttcatgagt 14760 tttgacatat attgtttatg ttcttccgta tggtgttttc cgtaaaatgg attaccttca 14820 cctgattgaa gttttgccat tttacgacgt tcccaggcgt aagatttatt aacgtgtctt 14880 tcatgagtat ttgaattcat tcccatacat ataacggctt taattatacc gtaatggttt 14940 ggatgaattt tagctaaaag tttatgagct ataaaatgct cttcagctgt tagttctacc 15000 aaattacact tatcatctgt accacccata catctaggaa ttatatgatg agtttcagtg 15060 tatccattaa ggcgagcccg attctgggct ctattaatta aattatcata tattaattta 15120 taattcaaag tttaacctct ttcatcacat aattaaactt ctcatcagca taacgctgaa 15180 tacgatcaag tccatgtttt aaaagatagt ttaaatgcga gtatttttta gaagatgttt 15240 ttgatttaga aactacgccc gcgtcatcta tgaggtccca gaccgttgcg attgttttag 15300 aaccatgctt acgtaatacg cgacctattg tttgtaatac aataatttta gatttaacac 15360 cgtgtgctaa aacaacgtga tgcagatttt taactgaaat accggtagaa aatacaccat 15420 aactagctac tataattatt cctttaccgt tttcagctaa ggttttcatt atattgcggg 15480 tttcggtatc aacttcccct gatacgtaat aaactttatc gtattcattt ttaattaaat 15540 cgaaaatagc tttaccgtgc gatacgtgtt taaacatgac aaaagcattt tcatcttttt 15600 gcgcaagctt aatagctaat ttagcgatcc atttatttct tttactgagc ccagtaataa 15660 tttttatttc ttcttggtaa gtttttccct ttaatttagt agtgaactca tcgggatagc 15720 gaagaaaaat actattaatt tttaactcag ttacttgtcc atcttccatt aatttagagg 15780 tcgttactgg cttaaatatt tcaccgaaca ttccaacata ctgcattata ttagctttgc 15840 catcgcgcaa tgaaccagac aaaccaaatt taaacataca gttatttaaa cctgatatga 15900 tagatgaaat actttttcct gtagcaagat ggcattcatc attcatcatc attccaaact 15960 gtgagaacca ttcttttggt tgttttacta cagtttgcca tgtaccaaca acgactggtg 16020 catcattttt atatttatca tcttttgatg ctccaccgcc aattttcttt atcattgcat 16080 gactgaataa acgatagtca acaaagtcat cagccatctg agttgtcaga gcagttgttg 16140 gaacaatgat aagaattttg ccttcataat tttccaaata ataccgagca agcaaagctt 16200 gaattaaaga tttacctgcg gatgttggaa gattaagaat tctacgacga ttaactaatc 16260 cttcgaacac tgcatctttt tgataccaat gcggttcaat tcttttattt cctgaataga 16320 tttctaattt agaaagccat tcatcaaaat cttttcttga taattcttct ttttcgttaa 16380 tctgtgggtc aatccaggct ttataaccaa agttatcaca gaacttttta atttgcccga 16440 ctaagccgaa tggaagaagg cgattataat ctaaaagacg gattcgtcca tcccagttgc 16500 catatctgaa gcgaggatta aacctatatc catcggcctc aaacgaaaag aaatctctta 16560 attcgtggaa cgtgctctct tcacaatcga tgcgtacatg actaaagtcg tgaaaatgta 16620 ctttaatatc cataattatg ccttactaaa tttgccttta gagtctcttt tcatgagacg 16680 acctttaata aatccatcgg gaataataca gtctggttgt attaatttat ttattgctcc 16740 attattgacc caaaaagttc ctgtggtagt cggtttgact ttacatcctt ttctggactt 16800 tctattaaga tgaaccatcc cttttacaaa tccttctgga acaagttctc cgggtttaat 16860 aaaaatattt ttagttccat tggtgtaaca agttgtacct aataccgtgc ccggtgagtt 16920 ttcaaatctc ttagcggaag attctttcat ctttgctata acctctgcag tcataacaat 16980 tccaccaatt ccaccaggtt tcatattata ataatttttg ctttttatta gttcaggagt 17040 tataatttct tcttcataca tgtacgcttc ttcggaagtt ttaaactctt ttagtattgt 17100 tctagagaaa ttcttttcac catatttctt tatagcctgc tgaattgcct taccggaacc 17160 aaggtagcca tcattcaagt catcagtaga gtgttttcct atatactttt taccatttat 17220 tagatttgtc gtttcatata caaagtgata catactattt tccgagtaat aaatatatct 17280 atatttatac tgaggaaata ttatgataga taaagattat attgcagagc tgaaggctct 17340 tgatgataat aaagaagcta aagctaaatt agctgaatat gctgaacagt ttggtataaa 17400 agtcaaaaag aataaatctt ttgataatat cgttaatgat attgaagaag ccctccagaa 17460 gctcgctagt gaacctatgc cagagactga tgggttatct attaaagact taattgatgc 17520 tgctgatgct gcagacggat taaaatatga cgatgaagaa gtcaatccag aggcagcact 17580 tctgattgat tctccggtta aatctgacat taaaattgaa gtagtagaaa cggataaaat 17640 tcctgaaaat accgatgttt tgattgaaga tactcctttt gttgaagaaa aattcgaaca 17700 ggctgtagct gagattattg aatctgagaa gccgtctgta tttactcttc cggaaaactt 17760 tagtccgaat cttcagctga ttggaaaaaa tccaggattc tgcactgttc cttggtggat 17820 ttaccagtgg attgctgaaa ctccggattg gaaatctcac ccaactagtt ttgagcatgc 17880 gtcagcacac caaactttat ttagcttaat ttattacatt aatcgcgacg gatcagttct 17940 aattcgtgaa acacgtaatt cttctttcgt aacattaaaa taaggataac ttatggcttt 18000 tacagttgat ataactccta aaacacctac tggagttata gatcagactc agcagtttac 18060 tgctacaccc agtggtcaaa ctggaggtgg aactattacc tatgcttgga gtgtagataa 18120 tgctccgcaa gaaggatcag cggcaacttt tgattatgta ttaaaaggac ctgccggtca 18180 aaagactatt aaagtcgttg caacaaatac agttccagaa tctgaatctg aacctgaaac 18240 agcagaagct acgacaacta tcacagttca aaataagaca cagacaacta ccttagctgt 18300 aactcctggt agccctgatg ctggagtgat tggaactcca attgaattta ctgctgcctt 18360 agcttcacag ccatcaggtg caaacgctac atatcaatgg tacgttgacg gttctcctgc 18420 gggcgaagca actggcacta cattcaatta cactcctgac gcaagcggag ttaaaacaat 18480 taagtgtgta gctcaagtaa ccgcgacaga ttatgatgca aaggaagtta cttctaatga 18540 agtgtcactg actgttaata aaaagacgca gacaactacc ttggcagtaa ctcctgatag 18600 tcctccagca ggagttattg gaacagccgt tgaatttact gctgccttag tttctcaacc 18660 cgttggagcg tctgctacat atcaatggta tgtagatgat tctcaaattg gtgaagaaac 18720 taactctaca tttaactata ctccaactac aagtggagta aaaagaatta aatgcgtagc 18780 ccaagtaaca gcaacagatt atgacgcaaa aacagttact tctaatgaag tatcattaac 18840 agttaataag aagacacaga caactacgtt agccgtaact cctgctaatc ctgccgccgg 18900 ggtaattgga accccagttc aatttactgc tgccttggct tcacagccac cgggagcgtc 18960 tgctacgtat cagtggtatg ttgacagttc tctggtcgat ggagaaacta ctactacatt 19020 taactatact cctaccacag ttggagctaa aacagttaag tgtgtagcgc aagtaactgc 19080 agaaaattac aatgaaaagg aagttacttc taatgaagta tcattaacag ttaataagaa 19140 gacaatgaat ccacaggtta cattgactcc tccttctatt aacgttcagc aagatgcttc 19200 ggctacattt actgctaatg ttactgatgc tccagaagaa gctcaaattg aatattcatg 19260 gaagaaagat tcttctccag tagaagggtc aactaatgta tacaccgttg atacatcatc 19320 tattggaagt caaactattg aagttactgc aactgttact gctactgatt atgatagcaa 19380 aactatcaca gcagaaggtc aagttcaggt aactgataaa gttgctccag aaccagaagg 19440 tgaattgcct tatgttcatc ctcttccaca tcgtacttcg gcttatatct ggtgcggttg 19500 gtgggttatg gatgaaatcc aaaaaatgac cgaagaaggt aaagattgga aaactgaaga 19560 tccagatagt aaatactacc tgcatcgtta cactcttcag aagatgatga aagactatcc 19620 agaagttgat gttcaagaat cacgtaatgg atacatcatt cataaaactg ctttagaaac 19680 tggtatcatc tatacctatc cataatcata aggggcttcg gcccctttct tcattttgaa 19740 agcacacaaa acacactcag aaaatgatgt atataatggc accaactcga taacatgaga 19800 ttgattatga gaactgaggt tgtggtgttt actcttcatg agtctggaaa gtcattcatt 19860 gaaattgctc gtgaattgaa cttacaggca aaggaagtgg ctgtattatg ggctcgagct 19920 atgactgcta agaataaatt tgaaactcga gaaaaagttg tctatagaaa aaggcatatt 19980 aataaaaagg tgaaaaatgg aacagtatga tctttatgaa aatgaatctt ttgctaatca 20040 attacgtgaa aaagcactta aaagtaaaca gtttaagtta gagtgtttta ttaaagattt 20100 ttcggaactt gctaataaag cagctgaaca aggtaaaaca tattttagtt attattgtac 20160 tgctcgcgat aaattgatta ctgaagaaat tggtgattgg ctgagaaaag aaggatttag 20220 ctttaaagtc aatagtgatc agcgtgatgg tgattggtta gaaattacat tttgaggatt 20280 aattatgttt aaaaagtata gcagtcttga aaatcattac aattctaaat tcattgaaaa 20340 actttacagt ttaggattga ctggcggcga atgggtagct cgcgaaaaga ttcatggcac 20400 aaatttctca ttgattattg agcgtgataa agtaacttgt gctaaacgaa ctggtcctat 20460 tcttcctgct gaagatttct ttgggtatga aattattttg aagaattacg ctgattccat 20520 taaagcagta caagatatta tggaaacttc agcggttgta tcttatcaag tttttggcga 20580 attcgctgga cctggcattc agaagaatgt cgattatggc gataaagatt tttatgtatt 20640 tgacattatt gttactacag aaagtggtga tgtgacttat gtcgatgatt atatgatgga 20700 atcattctgt aatacattta aatttaaaat ggctccactt ttaggtcgtg gtaaatttga 20760 agagctgatt aaattgccaa atgatttaga ttctgtcgta caggattata attttacggt 20820 agaccatgct ggattagttg atgcaaataa atgcgtttgg aatgctgaag caaaaggcga 20880 agtatttacc gctgaaggat atgtattgaa accttgttat ccttcttggc tgcctaatcg 20940 caatcgtgta gccatcaaat gcaagaactc taaatttagt gaaaagaaaa agtctgataa 21000 gcctattaaa gctaaagttg agttgtcaga agctgataat aaattgctgg gaattttagc 21060 ttgttatgtt acactgaacc gagtaaataa cgttatttct aaaattggtg aaattggtcc 21120 aaaagatttt ggaaaggtga tgggactaac tgttcaagat attttggaag aaacttctcg 21180 tgaaggtatt actctaactc aagcggataa tccttctttg attaagaagg aattagttaa 21240 aatggtacaa gatgtacttc gtccggcttg gattgaattg gtaagttaaa taaaaaggga 21300 ccgaaagggg tccctttgtt ttattcatca atgataattt ttggtagctt aacacctaat 21360 aaaacagaca aatctgaacg acccgccatt ttatccatgt ctccaccgtc aattactctt 21420 gcttcttttt catcttttgc tacagtgtaa ggatttgcag ataaagcata tctaactaat 21480 aaaccgatag atggctgcaa actttctgga tcaactacaa ctttaaatgc acctacatgt 21540 tcagggtcat ctaagtcaag gccttctgta tacggagcat agaaaattga tccaacaatt 21600 tctttttcac caatattttc tactacaccc acgattacat aatccaatgg gctgttggta 21660 tcgcaataaa gcggtaaacc attagctaag aacccgtagg cattttgtga aagatatttg 21720 tcatcttctg gtttatgttt taaccaacct gatgcagcaa gaatcgcagc ggcgcgagct 21780 gaagcaacac agaacgttgc tgtataagtt gattctttct ggatatgtga aaccatttca 21840 cacaccattc ggtataatga acgaccagct tcaggtgcag atgcataact caaatcaatg 21900 aatccagtat cagtaattcc tgtaacttta tagcgttttg atactgtaat caaagactgt 21960 agaatatctt tattgatttc atccgccatt tcggttgcaa gcaaatcttc caagaaatta 22020 ggagcatcga atccatttgc ttctaaatct tgtgctaatt caactgtgat gccagtttta 22080 agtttacgag atttaactgc ggtttgccat ttattaatct ggaatctagc atccgcaatt 22140 tcactatcag agctttcaaa tttacttgtt actgcagcat cagaaaatag acgaaccttt 22200 aaaagaacga ttgcaatctg aagagctaat tctaaatcgc tttcttcaat atcagcaaat 22260 ggtgtatctt ctaatacttt ataaacgata ttattatatt tgaataaatc gcctttattg 22320 agagttaatt tagactcttc tgttaattct gtgatttgtt ctcggtctac atacccagct 22380 tcgccggcgt aagtagcacc agttttaaat gtaaattcgt tatctgggtt aaggtatttg 22440 ataccataaa aagcagcaac aggttgatta gttctttgcg ttgctacaat gtcagaatat 22500 attaatttag tggtagcgcg agtcaaagca acgagatttg ggcgaccgat tgagttgcta 22560 ttcgttgtgg ttgattcgcg cagaagttcg ttgattttag ccattgcgct ttccttttgg 22620 tttatatgat ttatttatac cataaaaaca actaaaggga cccgaaggtc ccttaaatcg 22680 tcaaagatta gataccttta acatatacac ggcggaagta agcgttttta ccaaggctat 22740 tcagaataga aggcataccg ctctggatgc gagaagccgg agcctgagca gcggattctg 22800 caaatgggtt gataccgata ccgtaacgag ttttgaatcc cattactggt tggaagttct 22860 tcggatcgga accacgcagc ggagtcagag ctacatatgg agcgtagtaa ataccagcat 22920 ccatttcgtt cggaccttta taacctacag tgaaataatc ctgtttagca tactggtcga 22980 tatatacacg gtatttacca cccagaacac cagcaaatac tgacttggta gtatcagtgt 23040 taaagccggt agccagaccc tgtgcagcat aagaaatgcc agtatcaact gaagccagaa 23100 cgttaactac gttacgggaa gcgataatga agttaccttc accacgaccg gtctgacgag 23160 caatttcaac tgcttctttg tcaatctgga acaacagagc tttaaaggat tcacccgccc 23220 agcgagcacc acgaatatca atcggatcct ggaagtcaaa tacaccagct ttagaacccg 23280 gagtcagggt cataccagat ttaccaacct gagctgagta gttaatccaa tcaacaactt 23340 cacggttgat ttccaacatg atttcggtag ccagaatacc agacagttca gcatcagcat 23400 ccataccgtg aacagcgcgg aggtcttgtg ctaattcgat agagtaagca gctttcagct 23460 gacgagattt agcttcgata acttgtttat cgatacggaa gcccatttca ttccatgggt 23520 tatcggttga accgttgaaa ccttcctgaa gttcagcgat agaagtagcc ataccttcag 23580 cgatttctac cagtacacca gcttccattt gtttcttaat ttcagcatct aatttagcag 23640 cgtcagttgc gcttgcacta attgttactt gagcagaagc ttgcagatac acagtaccag 23700 tgtcctggaa gaagtgagta tagatatcac ctactgtggt ttgtgtgcta gcagccagag 23760 ctgtgaattt cttagcagca ccctgaccag agaacattgc atctggacca tacattgggt 23820 ggaatgcttc tttagcgcca gcagcgattg ggtctttacc atatactgca cgcagtgcga 23880 atacctggcc agtcgggctg ttcatcggct gaacaccgca aatatcgaaa gcaatcaggt 23940 taggaatagc acgacgtacc atacccataa cagctgggcc aatctgagtt actgcgccag 24000 aagtttgacc agcagcgatg ttagtagcgt tgtaaccgtg gtcaccaccg atttcagctt 24060 ctgttaagaa agaaccgaac gcctgagcga ttttttcgtc tttgtattct ggagctgtct 24120 ggaaatcttt ttcctggttt tcaaagattt tagcgataat cgcttgtttg ctattagcaa 24180 tttccggtaa accttcacct tccagtaatg gcttccattt gttcaaaagt tcagctttag 24240 ttttgatagt catttgtgtt aacctttaaa attagaaacg agatgcgact ttcgcatata 24300 cacttacaat atcttctgca ccaggtgcag ctttatcttc tacagcttca gtgacgaaat 24360 tcagtccggc tgcttcagta tcaggagtat ttataccctc agtaatagtg ctttcatctt 24420 tattagactt cttcaccatt tctacgatgg cactcaattt acttgagaat gcatctgaat 24480 aatccatacc ttcgaccaga gcagagactt tttctttttg agactcagtc agatctttag 24540 tactttcgct caatgccact tcacgctgca cataattgat atatgcgtcg cgcatattga 24600 gttcttcgaa cagacgagct gattcttctt tatgttcttg cagctcttct tccatttcag 24660 ctacaacatc aactgattct tctggaacaa cgacgttgtg ttcaacaaag agctctttta 24720 atccaccaag catggattca aacagttcgg ctttgatgcc tttatcaact gctaatttat 24780 tttcagcgag ccattctttc gcaagatggt cgatgaattt agaagcttgc tcagcgattt 24840 tcttctcagc tttttcttcg gcttcttctt tatttttttc tacttcttct tctgcttttt 24900 cagcaatttt agcgatatga gattcagcta atttaacggc gtgctgcttg acggtagctt 24960 cgaatacagt gccgaaagtt tcttttgctt ccggagaaat attaactgat tcgaaaatac 25020 tatcaagagc aacggaagca tcaatttttt gcgcttcagc aatcagttgt tctttaagca 25080 ttttgtagtc ctgttgttta gataataata tttataacgc ttttttcatg gcctctgcga 25140 gagccatata ggcgtcatcg gcacttgtat cggcttccgc cgtctgtgat tcggtaattt 25200 ccttaggagt tacccatgca tctggagcac ttggacccca tactgcatca acacctacag 25260 ttaatttgaa tccttcgttt acgatacgat aacctttatt tgtgtcagtc aatgaaccta 25320 atccacgaga agaaactcct ggaatccatc cggcacgaat attagctgct aatttatctc 25380 caggaccatg gtcaccttca ataacacgag ctcgtccgta tacgtcattt cctttccacc 25440 acatatcttc tataatgata gcggcttgca tcgggtcaac attagcgcgt ggaggatgat 25500 ttaattctcc gagagcttgt ttagttaaaa cttgctcatt gatatagtct tttaccgctt 25560 tttctaatat gcgttttgga taaagacgtt tatttctatt gacgacttcc gcttgcatga 25620 atattccttc gatgtataaa ccaggtttta aaccgaggtc ttttccatca tgagattcaa 25680 gcattggcac gccatcaata atttcgccag gttgacccca agtttcaatt agtaattggg 25740 gttcattcat tagcttaatc ctaatgcttt acggcgttta agagcttttt tacgcttacg 25800 ctgagcacga gattgacctg ctgggttggc aatcttcgtt ttggtagctt tgcgagcaat 25860 ttgtctacgt tttgctttag acagcccggt ggtttgaaat gcattacgtt cacgcgtctt 25920 gcggtcttta gtacgtgtaa tttcaccacg agcagaaaca tgtttaacga tgaattcatt 25980 tagcggcatg tcttcattaa tagaagctaa tgcaattgct aaatcagttt catcatcaag 26040 catattctcg acaattgtat ttatatcgtc tttatttaaa gcagaagaca attcgtcaaa 26100 gcgaccctgt gcttcaggaa taagtgcttc gacattctcg agaactaatt catgagtttc 26160 agggatcaga agcattattc atcatcctcg tcttcgtcat cttcgtcaga gtctttgtct 26220 tttttgtcgt ctttatcatc gctatcttca tcatcctcgt cttcgtcatc atcctcatca 26280 tcaggttctt caccttcgat taagaaattg cgagcgatag cgattttttc ttctttaatt 26340 aaatcaatcg ttcttgcagc catggcttca gcaaagaatt tacgagcggc tacgaggtcg 26400 tttgatttaa tagcttcaat taaaccttcc attaaaaatc ctcttgttct tggtcggggt 26460 cttggaaacg agcctcttta gactcttctt caatttgctt ggcttcttgt tctatttctt 26520 catcagtcat ctgcaaaata tctttcatag cagttctgtg agaaatatat ttaccaataa 26580 atggttctgc catggttagc atattaattc ttcgttccaa aatttctgct tctttgagtt 26640 cagtaaagta gctatcccga tgaaattcta tcttaatatt atttatttca tcattccact 26700 catcttctgt gattatacct ttaagcaaca gatttgtttt aagcggatct aggaaaactt 26760 cttcaaactt gtgctgtaac tcacgaataa atttagcaaa cgttaattca tcacgtgtaa 26820 tgctagttcc agaatcaaac atcacaccgc cttgttggtc ttgcgggatg cgtgaaagag 26880 gaacacgtaa tgccatataa agagcttgtc taaaccaacg aacatcttcc atattaccag 26940 tattatcggc accaggaaga gtatcaactt ctgtcacagc tttaccatcg cggcgctgta 27000 accaatagtc ttcggtcata gacatattat gctgttgatt ttttatttta ccggtcgatg 27060 cgtcatatac tacacggttt ttcatcgtgt tcatgacatg ttgcatgtgc tcggcagctt 27120 tacgagcagg catattacct gtgtctacat accaaacacg acggtcagga gcacgagtaa 27180 tacgataaat gacaacagca tcttctaata attttaattg gttagcaggt ttaacagcac 27240 gatgcaaata cccgatgata tttttaccac agcaatcgac taatccagaa tgggcataaa 27300 cgatggcagc tttaggaatt tttatttttg tgccagcttc atacattcta ccatcacatg 27360 catatgattc gtgcgcagta tcatatataa aatactcttt gtaaccttta actatttttg 27420 tgccagcttc agtttctgtt ataatttcac gaacatactg aacttgacga gggtctaatc 27480 tacgtaattc ctttatgcct tcttttggac gttttggatc aataatttta tgaaagaaaa 27540 ttcttgaatc tacataccaa cgtctaaaat gatcagaacc ttttcgttga aacgatagat 27600 gatttaacac atcgctaaat tcatctaaca tcatattttt aatttttgga ctaaatttag 27660 atttatccaa atttaacgct acgacttcag tatcatcttc atagacgata gcgtctgaaa 27720 cgatttctga aactgcatta tctacttcat agttattcat gagattacga tatgtatcaa 27780 taagctcacg agtagttttc attcctggtt catatgaacc aaaaattgtt tggaatgcag 27840 cattataagg agaagcagct tcgttcgagc ttacttcaaa ttctcttgct ccatcatcaa 27900 gctttggggc tgtaatggaa acaagatctt ctttttcttg gtctttaaaa tttcgttcgt 27960 ccattttagc ccatggagca aacaaactta atacattaaa tttcattgta ttctccaaat 28020 gggaattata gttatattta taatggactt ctctgcttta agcaggatgg ggatttctcc 28080 ccattcattt tattcccaat aatcgagagc aagagttact tcaaaagttt ggatttcatt 28140 gtttgaatcc caatctaatt gaagttcacc cacgttagta ggccacaggc ctttaatttc 28200 gacttctttt gttacggttt tagcgtcacg agcatattgg cgaacaacag cgctcttttt 28260 atattctgct ggttttccac cagtaatttc gttcccttgc ccagcagcaa tgctttgcca 28320 atcaacaaac ttctggcgag catcatgtgc ctcatcgttc attactgtaa cagtccagtc 28380 atcgaatgta cgatcgcctg ctacgttaat tttacggttc ataaatccga ctggaatttt 28440 ttctacaata ccagctggta aagcagtggc tttacattga aacgtaaaat tttgtccaag 28500 ataagaaatt tctacttgga ataagttagg tcgagcaaaa tcaccagatt caaacgctcg 28560 tgttacatca tctacaaaca tattagcctc tgtagtattt atatccctat gtttaaccta 28620 gggcatatag aattaaagaa ttaaggatat agtgtattta tatggcctgc cgaaacaggc 28680 ctttagaatc attctagtaa atcccaaaga tctacactat ttctttttga cttttctcgg 28740 ttagattccc aagtgattat ttctaaatta ttaataccgc caattatttt agcaggaatt 28800 aaattatcaa aaccgtattt gatagatacc ttgtgatcaa gttggtaagc accttctgta 28860 ccacacactc ctatcttatc agcattcggc cattttgacc aaattttctt atgcttacga 28920 gtttctttat ataccaatga aatatattca cgcatttcag atttatttgg gttccatctt 28980 ggatgattta cgccatgcat acattctact gtttttcctt tattcgcgga accatatgaa 29040 ttattgcgca ttttatcttt atgacggcaa tcacgacaaa tatcagtgtt tcttccaatg 29100 cgttggttat attcaattcc acatttatcg caacggcatt ttacatacgc ggtactacct 29160 ttaatgtctt caactttaac atcaatttca taatacataa atccatgttt aaccgctcca 29220 agaggtgtta atttataacc ctttgattga taatgtttaa aatttgacgg tacaattttc 29280 actttaacaa acttagaaat aatcacttgt ttcctcgaga ataggggccg aagccccata 29340 atcaattaac ctgcaagacc agttaactca tcgaaatctg caccagtagc agttgcgacg 29400 aaattcaaag taatataatt tatactgcgc gccggttgga tgtagaatgt tgcaacaaac 29460 tcatttctat caattactga cggagtgtta tttgttgtat cgcaaactac acgatattca 29520 taaattccac cgagagcttt aattccctgc aagtactggg aagtttctgt gcggaacgat 29580 gaacgagtaa acgcgttgtt taattcgaac aaacgatatt ttgaactacg tccgatattc 29640 gttttcaaca tattaaacag acgacgaacg ttaatacgat caaatggaga aggaacagaa 29700 gtagctgttt tatcaccata caatacgtaa ccatcaccac ctgtaccagt tactgggttg 29760 atagcttctt ggtataaacg gtcgcgctga gcttggcgag tttcaatagc aagtttaata 29820 acgttaagaa tctggccacg attataacca gctggagaca tccaagtctg agaaacgtta 29880 tcagttctcg cgcataaacc agcaatatca gctgctaatg gaacccaacg atttacatca 29940 ttatatttgt catactgata cttgtagtta ccatcaattg ctgcgtaggt tgaactgata 30000 ttaaagttat tatcagtgta tgaacctgct gcagttctcc aattgactaa gttatcaaca 30060 gcacgagtta caggaattcc aactacagtt tcacgcggtg gcgagcacag tactaagcaa 30120 tcttggcgag catccccaat tgaaacgacg tgtttttgga cagtagatgc tgtttctaaa 30180 gattcaccgg cacaagaacc cgcaataaac aactgaacat caacagattc acggtcagca 30240 aagaagtccc aagcttccat caaatctcct gctgttactt cagcatttga tgataatcca 30300 ccagacagag ttaaaattcc agagaagcct tctggccagt tttgtgcagt tgcgaaaata 30360 tattctgaac caccttttgc gaaaaagtcg tcaatataga tgttactatc gtaaatatct 30420 ttttcacctc gctttgtcga aagaacaacg ctttgaacaa tagcatcatt gcgacgaact 30480 atgatagcgt attgtgaatc agtttgtggt ccatatccaa acactgcttt ggcagtagat 30540 gcgcgtgttc cgccacctgg ataaattgga agtaatgcag aagcaccttt tgcgtagtca 30600 gctttagata cgatttcaat ttcgatttta tcgcccaact cgcccggata aagagctact 30660 actcctggaa ttccatattt ttcgagattt gcctgaaagt caacagctgt catagcagct 30720 tcggcatttt caatttcagc taataaaata ccagaatcag taataatttt tccaagagtt 30780 attactgcgg ctaaaccaga ggaagacgaa gaaatttccg cggtccagtt agaacctaat 30840 gctggatatt cgcctacttc tttcgcttta gcgataattt ttgcggtagg aatattaatt 30900 ttcttaattt ttccatcagc atctacttcg gtaactttac attctgtttc aatgtcttct 30960 gaaacatact taaccgtgat tttatctcca accgcgtagt tactacctgg ggtagaaata 31020 gtgtattcaa tattaccggc aatcggagat gagtttttag cggtatctct atcgacagca 31080 cgcacaactc gcaaatcatt tccgtattgt aagaaattca ttgcagacat aaaatagtca 31140 gcagtttcag ctgtaggttg accaaaagta ttaactaaat ctacttcatt tgtaacctgt 31200 ttaatttgaa aagcaggacc ccactggaat ttaccggcca aagctgctgt accagtagag 31260 ttattaacca cggtgctttg aaccgtagtt tctttgagct caatgcccgg agataataaa 31320 gtcattttta atcctcttta atatgcttta atatatttat accattgaca taccatgaga 31380 tactggaaca tactcagcag aatgaaccga atcaacaaat atgactggag cgtattcatc 31440 acccatatct tggagttctt ttgaaaacac ttcagatgct agtcgcatgt catctttgtc 31500 agcataatca ataaattttg attgtgttga taaccatcca aaaattacta aagacattac 31560 taaatcgtca tgataacctt cttcagccgc ccaagacacg cctttttcac taaacgttct 31620 aaactcttga atagttgcgc ggtgatgaat aataagctta tctttttcaa taaggtcttt 31680 taatgtagag catcctactg ctttcgttcg tttagtttgc ttcattccta aatcagtata 31740 tgaatcgcaa ataacgcctt cgtattctaa atccatataa agtgatttcg caactgacac 31800 accagtacta tttaattcaa tataaactgg gcattcatta tattctacta aataacgcat 31860 aacgatgtcg ggtagaatta aatgagaaat agtgtttgag tgtaaaacac caacttgttc 31920 ccacacatca tcggtaacat caataatatg taaagcgtgg taatcttgcc cacgaccttc 31980 tgagcagtct agagttgcaa tatattttct atctggttca gggcttttaa atcgatgaaa 32040 accatgatca tctggagtta cttcaatgaa atccataaca gccaatttca ttcctgaaat 32100 taatgtacca gaagtccctt caaacgccgc agtgtgttct tgacgaaatt gagctaaagt 32160 agaaccatta atggtttgta tgctccattg ccatccatcg tcaaaaatat cttcatcgtt 32220 ataaagacgt tctttaactg aattccaaat agcagtgtat ggttcaaacc ctgatttacc 32280 ttcaacagca gcagtccaaa tatcataaaa atgatttaat ccattaggag tcgtagtaat 32340 aataattttc gaacgacgac cagatgaaat tactggctga atagcaagcc aggaatcatg 32400 gaagtttgga ataaatgcgc attcgtcgat ataaatcatt gcgaacgagt taccacgaac 32460 tgcgtcagga gaggaagcat aggctccgat tgaggaacca ttatcaagtt caatagaccc 32520 tttattccat tcaacaattc ctggctgtaa aaagtcagga agcagttcaa ttgcttgctt 32580 agtacgatct aaaacttccg cagacattga gcctttgtgc gcaagaatac ctacagcttt 32640 atccttatta aagcatacaa agtgtgcaag aaaaatagct actacagttg ttttaccaag 32700 ctgacgtgat aaattacaaa cagtcatacg tttagatgac attattttga gcatatcacg 32760 ctgatagtca cgtaattgaa cctttatgac accatagtca atatgagtaa tggcgcagta 32820 tgtttctgca aaatacacaa tatcgtctcg gcattttttc cattcctcaa ccatttcacg 32880 agtccactgc gttttaatat tagctcgttt caagttagga agacccatat accgagatct 32940 tttattattc ttatctttat aagtttgaaa taattcaggc ttatcagaat tatttggaat 33000 ttttactatt ttgtgtagac gaagataatc actgaatttt tcaggatacc attttccatc 33060 ccactgtgat tttatccaat gaattccatc ttcatctttt ctttctgcta agctcgggtg 33120 ttttattaaa atttttccag cttcgtttaa cggatggaaa tcatttaatg cattaatcgg 33180 ttgttccatt tatcaccttc tcacgagctt cttgagcctc gtaagcatca ccaatttcgt 33240 ccattaattc tgttggtgaa cccatgaata ctgtcgcatt ctgaatattc atttgacctg 33300 taggaacagc gcctttggtg ccaacctgct cagatgtaat atctttcata tctttgtgaa 33360 gcttcagtat ttctctgttc gtcgtagtca tttgccccat aagagttgca aatacttcca 33420 tgtgacgagg agaatcagca ttctttgccg tctcaagaaa aatcttggct gcgtccatta 33480 gcatttgttg ttgaaaatgc atatttcgac gaactactcc ataatcatct tctaagtcag 33540 gagtacggtt ttgaggattg cttttaactt ctactaattg cagaggttca tatactttaa 33600 tttcctcccc gtcaattccg gggaggtcag aaatatctaa aagtttgttt atatcaagac 33660 cttccataat aacctctatg ttcttgggcc aggaggttct ggcggtgttg gtctatttac 33720 attgctagtg aaagtttgtt ttactgttcc atcccagtct tctgggttaa tatctcgagg 33780 aacaacttcg ctatctacag attcaaaaac accttcgcca tcaggcaaat ctcttgtatt 33840 ggcgtgaaaa tctgtataag tagtacgaat taatccttct gcatcatcta ctggaggata 33900 catccatcca tttacttcaa atgttagtga ccattcaatt ctacgacgag ataaattatc 33960 tccatctata gcttcatcta tagcagcaga catcagtaca attttaatat cccttttaaa 34020 tggaatatca tttccaaact gttcgtacat agttgtatta aaatgaggtt gaaaatatgg 34080 aagaatctgt tcaactattt gaaacatatc atcttcgtag cgagtaaaga tactcaattc 34140 ataaatcatt ttaataggag atggattata ttgtgatact acggaagttg cgcctttttg 34200 cagtaaattc tgatttaaaa tgtttgtttt aaatggagca ttatagctaa aatcaactaa 34260 atgtaaattt atacgaggta gaatagtttc aactttagct acatcttctt gcgaatttat 34320 cgatgtccat ttatttaatt tcatcatgaa gtgttccttt gatgcatacg taataggaac 34380 acgtataaac ttatcaccag attctaactg acgtttgatt tggatatttg aaaacaaatc 34440 gcccatcaag gtagcatatc gtctaaaaga cgaattataa aaataaccaa acatgatttc 34500 tcctaatctg ggctttaatt agtttataat atttattaat ccatgaaatc attatcaaat 34560 gggctagatt cgaaagattt gcctctatta ttgacaacaa cataaggttc aacatattct 34620 ttagcttcag aattaatttg atctacttca gcatactgat caatcttaat atcatgaatg 34680 ccattaagat tgcgaacagg atttaattct aattcactaa attctggaat gttaattcct 34740 tcatttttct gtagaactgg attaatttct tctccagaat aaatgaattt acctgctgta 34800 attttacgaa tagcgttttg gcctaattga taaaatggat catatggttc aacccagtta 34860 atttcgaata agctgttatc cataggaaaa tatattaaat ccccttcctt gggttctttt 34920 ccgttaactt ggtgtttaaa caaatttgga ttaatagaca aagttacttc atcctgtact 34980 tgcataccaa agttaccaaa gaatgattta gctccttcat atccttcaaa tgaatttaaa 35040 tatgcagcaa atttccaagc tttagtaaat ttatttttta ggtcttcgcc gaatatcaaa 35100 tcaggagaaa catactctct tggaacatag tagcattcta cacctcgcat ttgaatgctt 35160 tcagctacta atacatcagc taatatttgg ctgtttttat aatgattgaa atttacataa 35220 ggatttagta tttcagtttc attagtctga gaataaccag tgcgattttc taatttagca 35280 aaaagatttt tatcataagt agccatatta acctaccaaa attccaaatg gaggatcgag 35340 caagtataat tcttcgcgta atgcttcttt ttctaatcga gcttcttcta ttaagcgttg 35400 tccatcaatt gtaacaccgc ctggaagcat catgccttgg tgacgtgcta aaatttggcc 35460 atttaattct ttagctaaag ctgtagcata gtctttcacc caacgattat tataagcacc 35520 ttgcttaaca tctgggtctt gacccacaac acgacctact aaattatggt ctggattatt 35580 atatcgttca gataatgacc aactatcttg cggaccaact gttccatatc ctactgtatt 35640 tccaaccatt ttgtttgtat caatgtatga tttagtccag ctttctacga taatcaaatc 35700 atatttttgg aagtttccca tgactttgag ttgttcattt gctgaattga accaaaagtc 35760 tggaatagga gaaagcatat cttgcatcat tcccatgtaa ctggtaagct gggtaaaata 35820 ccctaaatca gctccaaagg cgtttggtcc ataaaatcta ttacaagaag tccccatgcc 35880 accattaata ccagccattc ctaaaagaaa gtcagtaaac catggatatg tagcgtttcc 35940 gtccattgac gttattgatc caacatttgt acgtaaaatt cgagttactg cgaatacgtt 36000 tgaacctctt aaatcgaaga ctccggtctt atatttttct tcgtcatctc ctacataaaa 36060 tacatgaaaa cctttgttaa gtccatcaaa atggtattca ccgtataatt ctagggcacg 36120 ctggatgcaa tcatagattt gatcgggtgt taactcaaca ttaataattg gagcccctaa 36180 acgtcttaga atgacatctt tgagttcctt tggattttga gaattatatc ctgacattta 36240 aaaatccttt ggggccttgc ggccccatgt tatgctggtg ataaaaaggt agaaagtaat 36300 acccattcgc cgtctttacg aacgtaagct tgaccatctt ttggagcttc aggaatataa 36360 cctgcttcct gtaaagtctg aacatccgcc tgtaatgaag atacattact tttaatacta 36420 cgcatttctt gattaataga agaaatgtta gtttcattag cttttattga attagttaat 36480 ccacgctctt ctaccgttga accatttgga tttgttccat ttactaattt atttaatgtt 36540 acaacctgtc ctttaattcc agtagtgtta ttaccaattt caacttgcaa gttttgaaca 36600 tcattgttaa gtctattaac agaagtttca atagttgaaa ccctatttaa aagagaccca 36660 ggaggagaag gctgacctcc actagaatca gttccaacga tttgatttag ccatgaaacg 36720 tttgctcgta gtccagatga agtgttttca ccaacaattc catttaaaaa ctcaatagat 36780 gttgtattat ctttaatttt acctttaata ctagaaggaa tatcatctga gccaattgat 36840 gtttcaatga cagttaatcg ctgtttaaca cctccgcttt tatttagctc atcagttaaa 36900 gctgaaattt ctcgtgtatt agtgttaaca tctgtgatta ttgaagtatt tcctggatat 36960 ccaatagaag ttttgataga tttaatttcg gaattaagat taatttgtga ttgatctaaa 37020 ttatgcacac gtgaatatat actttcacta aatgaaggcg gtctttgtcc taattcctgt 37080 ctaagatttc ctacttcgat tgtaagcgaa cctacatcag attcaataaa tttttcttcc 37140 aaatcactta gacgtattcc ttgggaagtt atagcatctg tattattaat gatacggtgc 37200 ttcatccccg tgctgctatt tcctacaaca ggaagaccat taatatcttg tccagcatat 37260 tgtcctagtt cccttttaat ccacagcaaa tcatttctaa tcgttctata aactgaattt 37320 gcttgcgaat taaatggacc aatatcagaa agaatattat caaccgtagt atttgtacca 37380 tttaatatat cagtatgttc acttgtgagc gtctttaaac ttgatatatc atttttatta 37440 acgctgatct gtgatagcgc ttctatatct cctgatacat ctaaaatacc ttgaatagtt 37500 ttaatatctt cctgagatgt ttcaatggca gttttaagaa ttcctacatt ttcgtcgaga 37560 acctcgacgt ttttaaacac ggaaacagtt ggtctattca ttgacccatc gtttccatac 37620 ttagtactag ctcctaatat ttcttcacca tttttaatcc atgaaatacg ttcctgacct 37680 tcgccaggaa cgctatcaac aaaaggtaac tcttttaatt caatcatttg taatccttag 37740 taatgaattt taataatata gtttaatgat atattccatg gtctagtttc ataaccaatt 37800 aatccttcac gattaagtgt accaagggca tctctcggtc cacctaattc aaatccatca 37860 ttagtgaagt atgaagcgtt atcccagtcg gcatattttc tagttccaag atatccttga 37920 taaacggacg cgccaaatgg agcttcatgt ctttggtatt ctccccagcc accagcatgt 37980 ttatggtatg acatttgttg tgcttgaaca ccgccaacat gcattccgtc acatcctacg 38040 ccaagtctat cctttccata accatcttgt cctcgttgat ttaaaatatg acctcctgtg 38100 ccagctcctc tgacgaaaag acctcgcata tcaggaatgc cagggttatt ccaatcacca 38160 ccaaatcttg ttccaactgt gtttctataa tcaggatatt gatctcctga cacggttcca 38220 ccatggcaca taatccaacc tggaggtgca gaatcacccg caaacatcat aatagttcca 38280 actggaaccc tttcagacgc atatctttca gtggcaacag gttgatcacc atttttccac 38340 ataccgccgc gcgaccaaat tccatttgct gtaagatggt ctaaatttaa agaaccatta 38400 atagtttgtc caccgcgtgt attaattaca tctgcgttcc atgctaatgc tccagagcca 38460 tcaccctcgg gggcaatacc ggcttgcgtc gttaatttta ctacaccgcg catagaacca 38520 gtagctcctc gaccatttaa tgtttcgcct gttactgcaa catctcctaa attactgtta 38580 atttccgatt gtgttcctaa acgtataaca cccttatatt cttgtgttgc aacagaattc 38640 ataaaggtat acggagaaat tgcatatcct tcgcggagag ttccttgacg agtttgcgca 38700 acagtagata attgaactac acctctcaca gattctgatg cagtatcttc ggaaggagct 38760 atttgtgaaa ttaattttat tgcaagtttt tgcgttttta atggagtcat tgccgtagta 38820 tcatcaactc cagctaaagc tgcaggtgta gatgatattt taataactcc atttgatgat 38880 tcagatgaat acctgttttg gaatacatcg tcagtatggt atttcaattt ttgtggagtt 38940 atcgacgaat tattatctga gccttctaaa gtttcttcat ttgttgaata gcgtgttaaa 39000 ccatatttag tttcagtagc atttggatac aataatcttg ttgctaatgt agccggtgta 39060 acgattttag aattattagt tccatctaaa acctcttgct ctgacgcaat cattgctatt 39120 cctttaactt ccatagttgc atcaggaata ccatttacgc cgatattact tatttttgat 39180 aatgcagact gcactgtcgt gacagtgtca ggaaaattcg atcctactgg atcgaattta 39240 acatattttg attcatttga tacgtgctga tatgtattgt tactcatgct attctctcaa 39300 aataataaaa tactgtaggt tgtgtgtgac tatctagcgt aacagtttgc gcacctaata 39360 attgccaagt tccataccca ggtttagaag gagaaattat ttcttgtttg aaagtaattc 39420 ctttatcaga gtattgttct aagatatgtt cttgattatc taaatactta acttgtattt 39480 tatttcctat ggtaggatgg tcttcaaatg aatcaattgc aataaatttt gcaaccattt 39540 cctgcagagc tgttctaacg gcactggtaa attcgattga agtcattcca ttagtagctt 39600 taacaggaat gccaaacatg ttaacaataa ccgggtcacc tggtttggct gaagtatcag 39660 taacagttcc ttcaaatgac caatagtctg tttgctgcac gccagtagga gatacgccac 39720 ttatattaac taatacagct ccaacctgtt gctctgccat atttctaaca tcattaatag 39780 cagattctac atttgggtca taaaaccctt ttgctatttg tgaaattgtt acagcaccta 39840 ctggctgatt attcataacc gaaatatcat ttttcttagt tctaaaacca agaaaatctg 39900 ctaagcggga aataactccc gctttattat taagtaaact cattatgcaa tccttatcca 39960 acggtataca gtaatagacg gttgaatatt actaatatta gcaggaggag tatgtgaaga 40020 gtttgttgtt gcgtaatctt cacgatattt tgtatatata ggaccagttt catctgggtc 40080 atattggcaa cctccaataa taaccgatcc attttcgtct tcaattaaaa ctctttcgtc 40140 ggttttagtt gcaggaagat ttgcgttttc aagcgttact gatgttgttc caaccgttcc 40200 acctgccgta tgtgaaggat ttccgctaga atctaaatca ttattgttta aagcaaagtt 40260 tggatccgta acatcatcat tccatcctac taaaacttgt cctttaccaa ataatttcca 40320 tgaaccaaat cccatataag tcaccggatt atttgggttt atagcatttt cataaattga 40380 accaacagga taaatgctgt cgaaaataga tgatattgaa ttatacgttc tagtataaga 40440 atcaactttt tcaacatttg gccaaccgat tttatcaaaa tctgttaagg ccacatcacc 40500 agtaacttca gtagatgatc ctttactaac ataacggtca tcagtcaatt caatgatgtc 40560 atctttttct aaaagagttc ctaaatcatt attgaaccat gttataacga taatgtcacc 40620 ggattcaaat tttctatcaa ataaaagact gataggtttt ccgtcttcat attctataga 40680 ataatcggtg tttgattctt tccattcgcc accaagagat atacatcctt cttgcgtatc 40740 tgaattagcg ccttcgcaca aaaacagagg atatccagct gttccagcct gctgctgtaa 40800 aattccatta aaacgaacct caagagagtt cggattaatt ggttcaccag gaattaatcc 40860 aaatgcagaa aatggaattg atttcatcgc cgataaatca gtaacataaa tgcttccttc 40920 taaagaagtc tttgatgtta gttttgaatc cagtactttt atttggcgtc ttgtatatga 40980 acttctccat tgtgatacac catccataaa tgtttcaatt tgaacagtgt caccaatatt 41040 acaaggctgt cttaatcgaa tattaaatcc atcaagagga actaattcgc cttcattttc 41100 gcccggagaa ccaaagtcac tgttttcgct aaatacatca ccgtaatata attcgttacc 41160 acgatgtttt actctgatgt tattgacatt ataactagtt ccacggaaaa tatctaaaaa 41220 gtcagtttgt ccttgaactt caactaaaaa ttctttacga gctacattac taatgtctga 41280 actgataatt ttgtcaattt gcttattttt gacatattcc cagcgtcctg gagcacaata 41340 aactaactcc aaatcactga attgaatatt aatttcaacc gatgatgacg accctttaat 41400 tgtatcgcca gaagcagcta ctagtgtaac tggattaatg ttccatgttg caaatacatc 41460 cctagctcta attactttat tataatcatt aacagttcct ttaggaagtt gtagtgttac 41520 tctcccagac gaagtattaa tagcgtatga ttttccccat tctgccgtta gtgtttgccc 41580 agatgaagcg ttataagttt tccaggcacc ggctgaatat ggaacatcac catcaccgag 41640 ttcataataa agctcatcaa agttttcatt tatttttata ccacctttac gcaggtagtc 41700 accggtacca tcatctacaa cattaccgat attaatattt tgtttcatta ttgagccacc 41760 ccgattttct gcgtagcgat aactttaact gctgctctca tgccaacaat agaagaactt 41820 acagtcgctg ttacataatt tgttttaata ctaaatgcaa tattagcgat ttcgtcttct 41880 tcggtttcat ttccaacccg catgacagca tattcagaag aaattacctc tgaattaaca 41940 gtatctacaa gaatatttat ttctgctgtt ttaatttttc ttccatctac cgattggcat 42000 gtaactagca atttagccat attgtattca gtacgatgaa atagtggaat atcaactgac 42060 ccggatgtag aaatattcca tgtaccttca gctggcgatt ccttttggcc aaacatactt 42120 tcaatagaat aattccaaac agacgtagaa ttatcagatg aaatacagcg taaagttact 42180 ttactatatg gactagttac tactaaatta cctgaaacac cttttattga atcaatcgct 42240 tgaatcgtta aaggattagt aactgatata gatccatttg agttaataaa ttcaacgcag 42300 tcaccaagtt cgcctctttc aatgataact ttaacaccta cagtagaggt atcaatatca 42360 tgcctagtac caacttttac tggagttgcg tactctgcaa tagagtgttt ttgataatat 42420 ccagtagcat gaataatttg gccatctgct ccagtgccat ttgctactgc cattttacgt 42480 tgatcgccaa acgcattata aattgcgtta aaatcactat taattttatt accaccgtcg 42540 aataagatat caccagtaga agcgttacca atttcgccgg tatcaatcaa tttctttggt 42600 tcttgaatga acatagcggt ttccttatga gtttatagta tttataaaga aaaagggagc 42660 ccatgggctc ccttaattta aaatgtaaac agaatattga tttcttctgt ttgatccatt 42720 gccatgataa taggtggcct attttccata taaatcattt cgcccgaatg cctcattaaa 42780 tcttccggat cataataatc cttttcagct ttaacgtttg ggtcatttgg atgaacttta 42840 gcttcaagag gattggtgat tattgatatt tgtctaaatc ctttatttcc tggtaatgca 42900 gcatcaggaa agtaaactga atctaaatat gctttaaaac ggatagtatt tgccttaaca 42960 cggtaaatta atccaaaatc atcttgttgc caagtgagat tatcttcata cccccatcta 43020 gccgggtctt cttttaattc ctcaggccaa ggaactacga tatattcatt cgtgcatcta 43080 tttatagata catcaggcgg aatctcaaaa agatattccc aaacataacc gtcgccgggt 43140 tcgattgttc cttcagcgtc tcctcgacct tcaggaggag tcattgacct aacagaagga 43200 gtccattttc cacctaactt aagacattca tccttattag ttaaagatgc tattgaacac 43260 gttccggtat caggaacatc taaacaacga tatactaacc agcctgcgcc tgattcagta 43320 gcattgtaag gagctgagtt acacactaca atatcgttaa ttctaaatgt gtatggatcc 43380 ggatatctag tatctcccca atctctacga ggaataactg catcaagcat agatggaaga 43440 acctttacgg ttcccatcat atgagtccac atgtcagtta cacccaatac agaatcagtt 43500 ggataaggtg gagcaaagcc cacctcattt tcatttgatg accacggttc tgatcttcca 43560 aatgtgataa agatagtgtt tttatccgga ccacttccaa ttgaattata aaaattcaac 43620 attttttctg ttctaaattt tgaagtaact atcgcacgat agataacact tgaatcattc 43680 atctatttta acctgtgttg gattttcagg gtctcttgga tttcctatat tatcttttag 43740 acgtttatta actaaatctc taaattgcgc aaacgttgtt ccagatgcgt caaatagagg 43800 actcattaat ttgcgtcgtt cagaaggcaa ttgaccttga aatattgaat tgttattttc 43860 agtattatag tcatcaggaa gaggatattc tacaccagcc attgggccag gctcataaat 43920 tgcttcgcct gttaccgaat catgctcaat ttcaccagtc ggagtcaatt tagctactct 43980 atcagcatat tcagtaggca atccagaatc ccatttgtag tttttgtatt tattaattat 44040 agtctctgta tgtttaagag ttaaaccaac attaataaac atcgttaaaa gggtaattgc 44100 tataaatcca aatcctactg gatgaacaaa acgaataacg tcagatttcc agcgggaaga 44160 aggcaaattg gatttaattt tcattacata atatgatcga cttctattaa tatagtctat 44220 attattttga agcaaatcct ttcctttaac tccacgaata atttcgcctt caaaactagg 44280 aagtctttct gctttaactt cttgaccagc aattaatctt cccaaaagat tatgaatagt 44340 tacggtccat tgcaatttac cattagaata gcttctttct atataagtaa cattacatct 44400 tcctgtagct gtataaatcg tttgtcctac taagtcttcg gtcaaagaat cagattgaac 44460 gattatatca tattcagtac cagccccaga ttcgatttca atttcaactt cttcattata 44520 aagaacttta aaaagaaatt tgtatgatgc ttcaattcct ttagtagaat aaaaatcata 44580 gctgcgtgat tcgaaaaatc ttgcgacagc atcacgtttg tcagcattta aataaatgtt 44640 tcttttatat atctctgacc acaaatattc ccatgcatgc tcttctcgtg gatatttgtt 44700 acgaattaaa tttcgtaaat tgttatactg agttccatat ccatcagaaa gatattgaat 44760 atatgcttcg caaaatgctt caaaattcga atcctgcaac aaataagaat caggcatcat 44820 tgtgccaatt aatggtctta aatcagggtc agctaatcca cgctcttctt caggcgacca 44880 aggagtttca tgttcttggt tttgtaaaaa tgcctttaaa aatactccag ttggcttcca 44940 aataatttct actgtatctc taacacgata attaaaatca tagtaagaaa ttatttcacc 45000 ggaggattta taaaaaagca tcccagatgc atatttttta aatccagtga atttaacatt 45060 tggcattact atagtgcaat cgccgtcatc ccagtattca tgaactaatc tatctggaga 45120 tgtttctgga aaattttcaa taactttagt gtattttaaa tcagcataaa ctactacaac 45180 tctatcagag ttgtttatcc aacatcgtgt gttagatttt ttagaccaat taaagaatgg 45240 ttctgcatag tatttcattg gctgaggcgt aaaagtttcc caatcagatt tttcatctgc 45300 tataaatgcc atcatatgat aatgcttatc agctaaccat tcacgaggaa attcatattt 45360 aacggcaccg attaactggt attttgatgc agtttcaggg tcattaacaa tattatcact 45420 taaaaattta aaattactcg aagatagcga aactaattta ccgtcagttg acatattcgc 45480 ataccctggt tgaatacgtc ttctttcttc ttcagtattg ccaaaaactc ttttccacgt 45540 ttttgtgtca tgatttaaaa catatattcc tttatcagca gaatcaatta tttttgatgt 45600 tctaggatta gcatttaatg tttcaacttc accaataata agagcaaaaa ctttatcacc 45660 gatagaatcc attttatagc atactgcttt aggatttcca gttatagtca tagtatcagg 45720 ttcaaaaagc ctttccgaat atgttggaga taatggatca gaatctatag gtgcattact 45780 cgtttttatg tatctaactt tgtctctggc gacaacgtaa atgtaatcat cggtgcaagt 45840 aatagcttct gcaatgcgat atacattcgc tggtaaagtc gcgtaagtgg caaaaatttc 45900 tacatcaaat cctaaatgta attgatcgcc aagtttagcg aatgttatat cctgcgaact 45960 aaatctgaca tcgtcagctg accatctgac atcagtagat ttgcggccat agaaaatctt 46020 gtcgtatcct agaacgtatg ttgtgttcgc agattggtaa tataccgtct tagataaagg 46080 atatcctaca cggtcattga agagctttac agctttccaa gtttgtcctt tatcattgga 46140 tactttaact acaggttgat agcgctcaaa aaggtataaa atcccttctg attccatcaa 46200 ataaactcgg ttaatatcct tacatacttg ctgaatagaa ccttgtattt catgatactc 46260 attttcacca ataataaaat tgcttattga tgaaacatca acatatgatg gactgaattg 46320 aaaagattca ttcatcaatg cagccattat agtgtcatta ttaaaattga cataaccaga 46380 attattaaga gtaaattttt cctgaatgaa tttattggct aattgcattt caatcatgtt 46440 ttgaaatgta taagcatttg tagcaaaagt ttcaaactct tcagtataaa tccaatcaga 46500 ctgctcaaaa tcttgtgcag ctgtagctac tctaataaca tatgatgtca atgggtcagc 46560 atcatcaaaa aagaagctgt tatttgcagt atatcctaag ttaatccatc tgtattgatt 46620 actcgggaga ttttcccccg agtttgtttt tgtctcagcg atttctacaa aatagtagaa 46680 attagcacca acgtcatccc agcgcacttg cacctgattt gcggataact tggaaattct 46740 gagactagtg actgaaggtg cttttactgt cattgtgata taggctccaa atcgatagtt 46800 aagtattgtg gacgtaagtc attttcaaat acaatcagcg aaccatcgcg agtaaagata 46860 acatcatcgg ttgggtcaga atataattca atagtctgaa cttcaaattt ttcagatgtt 46920 aaattaattt tagcgatatt ccaataaatc acatcagctg gataatttat ttcaccgata 46980 acatagtatt tgtcgcgtcc atcagaattt gctaatttgt taaaatcgtt gcctgaatat 47040 ggctgaatgt tttcattttc tgtaacatcg ccagaagcaa atggaccaat aataacttta 47100 ccaattcctt tagaatctcg gtctgttgat actatgcgaa cgtcatataa tacatcttct 47160 tctaaaccag tatcaggatt tacaaccttt cgtccagaat taaatgaaaa cgtattagat 47220 tccatagaac gatcttttat ttgattattg tatttaatac ctgcttcagg cgttttatag 47280 aagttttgta cttcacgaac aatttgaatt gtcgctgatg agccaatgat agaatgatct 47340 gcatcatcta catatgtcaa catcttagat ttagcgaaag atgagttaaa aatttctaca 47400 tcttcggtat aataacgatc aattttatca attatttgac cttcgagcca ctgttcggat 47460 tcttgcagct tatttaaagc atatgtgact tttaaattag tcttaataaa aagataatta 47520 ggagaaataa ttgacggtgt aataggagct aaattatagt ctttgagata atttttaata 47580 tcttcgcgct gtacggtagt taaatacaga cctgatttag gtttagcagc aataaatgca 47640 taaccaggtt tagtagaatc agtgaaagtc tgaactgctt gaataataga accaaatctc 47700 tctgaaacga atgtatcata gtcagtcgca gttacgcagc gttgctgggt ttcgcgttta 47760 atagtaccca attcgcgaat acgttcaata tcttctggat cgccgccgcc atctgcccca 47820 acaaaatctg ggtcatcgtt tggattttca ttaatgttga tgacagttat atttgtcaat 47880 gtatctgcgt atgaaaatcc gactgcacca ttcgcatctg caccgttagt actgatgtac 47940 tcaataacaa tcgtagagtt ctgggtaggt ttaagacctc caatataatt agcagtcaat 48000 gctccttctg ccgcattaac agaaatctcg ccttcaccaa aataaaactc agtattccca 48060 tcaatagttt cacgcatgta gtaaattgtt gatgtagaac cagcatgaac cattgacttt 48120 cttgtccagt taatccattc tgctccatca acgtatagtt taacttgatt tctatctata 48180 tttttgtcat aaatgataat aggtgttaat ttatcataga tgatttcagt tcttactata 48240 cgcccctggg ctaattttaa acgcggaaaa tattggttat ttttatcacg aatagcaata 48300 acatcttcgg tagatacaaa gttgtaagga ttaacggaag tatcctttgc atatgctaaa 48360 aagcgagtac cacgaggaat agtaatgtaa tttctattca atgcgtctgt gcaagttagc 48420 ataatttcag tttgagcagc tgattttgaa gtaggtaaat atccattatc ttgtgcagct 48480 tgaactactg aactacgcaa attagcagta cgcataaagc tttcgtacaa ggcagcatta 48540 ccaaactgct gaatgtataa tgtattataa gccaaaaggt cgcacagaac gtttaatctt 48600 gagccttcaa aatcataatc caaaaattca ttttggccat taagccattc aatgaggttt 48660 tgttttattt cagcaaatgt acccccgacg aatatctcgg gaatagcatt tgctgttctt 48720 gttaattgat aatttacagg ggtatttgcc attttaaatc ctatttaatg aatactttag 48780 atgatgcctg agccacagta tcaccgcatg atattggatc agccatttga acagctttct 48840 ttccagtgac atatacctta gaagttctag gttgtgtcac tccgccatgt gtttcatatg 48900 gctttttaat ttctgtgtgt tctgtaattg gatcacctgc tacgagaaca gcaattcctc 48960 cagtgaatac tttactttgt gtagcattca caactgttgg aggccatgct tcatggccgg 49020 cagtaacaca cttatcataa cttaatcctg acatttatgg cctctcatac acgtagcttc 49080 tcaatttatt agcccaacga ctccaatttc caactatagt ttttgtgtaa tttttaacga 49140 gagtttttct tattggagct ggaggatcag tcggttcagt agtatcagaa gatgacctag 49200 aattactgcc agaacctcca gattcacttt gttcttggta gtcatatatt aatgttactt 49260 cgtaagtgaa tgtcttctgg aggttttgag gagctttcca taaatataat tgagtattag 49320 aatcagttgg aaggtcttcc catgaagcag cagttttaaa ttcattatct aaacgatatt 49380 tcaatgcgtc atttccaaat ccaaatacag attcatacgt tccatgtaaa cgattttctt 49440 ctactaaaac cccaggagtt tcttcgtaac tagttatatt tatagatact aacgtttcac 49500 ctgtttctaa ttgagcggta aaggtgacgt cgatagaaga accttccatg gattctccta 49560 aatcagcgct cattggaagt atattagcca atgtcaatcc tcgatccatc aattgtgtat 49620 tgaccagatg aaatagaact catagatgcc attttttctg tccaatcgcc accaacgtcc 49680 caatcaactg tcccagcaac tttccaagaa agatttccat ttactgtatt agtctgattt 49740 ccttcaacta aagtggtagc atctccttta actgtaatgt cagcattacc ttcaactaca 49800 atagtaacat tacctttgac taggatagtt ccattacctt caaccgtctt agtttcatcg 49860 ccacgtacaa atattgtatt gcttccatct atttggtgga gacgattatc catgttgtaa 49920 taaatttctg atccaccgac gttagtcttt ttatcgccag ctaccaaaaa attaccatcg 49980 gcatttgtta tatcatacaa attatcaaca gtttttcttg ttcttcttcc tgacggtgat 50040 acttcctcat atgttccagt cggatgaact aatctgtatc gttcttgccc gggggtatca 50100 tcaaattcct gaatatgacc gctttcagtt tccattgtgt gaacatacgg atattcgcct 50160 ttatatgaag aaacaggttc tttgaataaa attctcgaat cattcggaat aggagggtca 50220 gctggatcag aagactttgc tacagcagca gccattgctg acaaagacct agccggtgtt 50280 ttcacttcaa taccgtatga ttccaaatta cccgtaagaa taatcatggt aacacgggat 50340 gcacggcctt ttgtttgttg ataccacaat gaatcacgac cagctttata tgccttttcc 50400 caatctccgg ctaacatagc agttaacatt gtgttaaatt tagctacacc accaacaccc 50460 atctgaaatg ccatattttc taatgccatt tgacgagaac gattaactgc ttgccaaact 50520 gggccaactt tagaatgtga tttaatgtca cgttgcatat cagccaaatc acgttcaaac 50580 aaagttaccg cttcttccat tgtaatagaa cctggatttc cggtaatttc acgaccaact 50640 tgttttgata aaactttatt aatttgagtc atatcacgaa ctggctgctt catgataaga 50700 tgaccaatac caattgtcgg atatccttca gtatcccaat aaacttttag tcttaatcct 50760 tcatcacggc gaagcatttc agccattgac atatttggat tatcatcagt tggaatttct 50820 gataatggtc tatcatcagg atttatcgca gtgtctagat tgctatcttg gataatatta 50880 gaagacgaat catatcctgc ttctccgcct tggtttaata cgttagtatc atttcctaaa 50940 cgtctaggat actgtccagt tgggtcagaa aatccttcaa gtctattagg cttttcgcga 51000 actattccac catacgtgcc aaggacaatt ccgttagttt tccatttgtc taaaaaatga 51060 ccataaactc tagttccttc taccggacca gtaacggagc ctccaattcc agacattgct 51120 gcagaagtta taggttgaat aactgacatc catggtaatt tatcagttgg aatacccatt 51180 acgtcgcctt gtgctctttg aggtggatgc agaccaacta cacgaacacg aacacgacct 51240 aattttaatg ggtccattct atcttcaaca acaccaacaa accaattaag gttattactt 51300 atcatttcca taagatttct ccattatacg tataaggtcg ttcataaatg aatttatgtc 51360 tgattttgct attattttta tttgacgaag tttttcattt tcaagaacag cagcttcata 51420 cgtatcgacc gcagcaagtg ctccttcata ttgaggatat tttctagctt tatcgccttt 51480 gtcataccaa acatatggat tatcatcgta tgatattaaa ttataaaatt tttcaccgtt 51540 ttcattcaca tgatatacta tttggtctcc acctacgttt ttgtattttt gtatagatgc 51600 ttgataagca gcttcttgcg aagtaatcca tccataatac ggatcataat tatcattaca 51660 catcaataaa acccaataca actgcggatt tccatatata gcatttgcta attcttccgg 51720 gcgtggtgaa cctttaatat aataagtacg taagcggtat cccgcaagag cacgtttgaa 51780 atagtcttta tagtttctaa aaatatctgt cataggaata gtcggcgcgt ttttattcac 51840 cgttttggcc gcatattcaa tcggatcaaa aaatgtaaag agcatgggcc ctcctgttta 51900 taaatatatt atctatttat aaggagaatc caatggcata ttctggaaaa tgggttccta 51960 aaaatatatc aaaatataga ggtgacccta aaaaaattac gtatagatca aattgggaaa 52020 aattcttttt tgaatggtta gataaaaatc cagaaattat tgcatggggt agtgaaacag 52080 cagtaattcc ttatttttgt aatgcagaag ggaaaaaacg tagatacttc atggatattt 52140 ggatgaaaga ttcttctggt caagaatttt ttattgaaat aaaacctaaa aaagaaacac 52200 aaccaccagt taaaccagca catctaacaa ctgcagcgaa gaaaagattt atgaatgaaa 52260 tttatacata ttccgttaat actgataaat ggaaagcagc gcaagcttta gctgaaaagc 52320 gcggaataaa atttagaatt ctaacagaag atggattacg agctcttggc tttaaggggg 52380 cataatggct atttttcaaa taattaatga aagcactccc caagttccaa aggttaagca 52440 atcattaaac gaaaagaaat ggattcagat aggtcttgaa tacaaaaagg ccaaagcaaa 52500 aggaatgaca ggaaagcaat ttgctgaaga aagaggaatc aaatactcta cgtttacttc 52560 agcaatgtca aaatatgctt caggaattaa aacagctgaa aagattcaaa agcttgaatc 52620 aaaaccaatg aataaactca ataagcaaga aagacaactg cttatgataa attcattcag 52680 gcaaacattg cgtgataaaa ttcgtaatga aggtgcagca attaataata aaaccaaaaa 52740 gtggtttgct gaaactatta aacaagtaaa aggacataaa gttgttcgcc cgcagccggg 52800 acgaatatat gcttttgctt atgatgctaa acacaaggaa actcttcctt actgggataa 52860 atttcctttg ataatttatc ttggtttagg taaacataat ttaatgtacg gattgaactt 52920 gcactatatt ccacctaaag ctcgtcagca atttctagaa gagcttttaa agcaatattc 52980 aaatacacct actattacta ataaaacgaa attaaaaatt gattggagtc aagtgaaagg 53040 atttaggggt gcagaccaaa tgattaaggc atatataccc ggtaatatta tgggtagcct 53100 tgttgaaatc gccccgaaag actgggcgaa cgttgtgtta atgccacttc agcagttcgt 53160 ttcaaaagga aaacgtttct ctgcaaacaa agtctggtca aatatctaat tctattatct 53220 tccattcttt tctattgttt gttctaattg gaattgaatg gaagggactt agacccatta 53280 taccaccaac atttataaag cattatgagg aatatatgtc gcaagcactg caacaaattt 53340 ttaaccaagc aaatacaact aactttgtag tatcaatacc gcatagtaat actacatctg 53400 cttttacttt aaatgctcag tcagttccta ttccaggaat taggatacct gttactgata 53460 ccgtgactgg gccgtttgga ctaggccgag cacaacgtcc aggagttaca tttgagtacg 53520 atccacttat cgtgagattt atagttgatg aagagcttaa gtcgtggata ggaatgtatg 53580 aatggatgct aggaactagt aactatctta caggtgaaaa tactgcccaa aaaacaggtc 53640 ctgaatacat tacgctttac attttagata atagtaaaac tgaaatcgtg atgtcaataa 53700 atttttataa gccttgggtt tctgaccttt ctgaagtaga atttagctac acggaagatt 53760 cagacccagc cttagtatgc acagcaacga ttccttatac gtattttcaa gtagaaaaag 53820 atggcaaaat tatagcagaa gtttaatgct tcagtttcat gtgttataat cttaactaaa 53880 tttgaggaga aacatatgaa actaatcttt ttaagtggtg taaagcgtag tggaaaagat 53940 actactgcag attttatcat gaataattat tctgcagtta aatatcaact tgctggtcct 54000 attaaggatg cattggctta tgcatggggt gtatttgcag caaacactga ctatccttgc 54060 ttaactcgta aagagtttga aggaattgac tatgatcgtg agactaattt aaatctgact 54120 aaatcagaag taatcacgat tatggaacaa gcattttgct atcttaatag taaaagccca 54180 attaaaggcg tatttgtttt tgatgacgca gttaatttcg tagcatttaa taagattgct 54240 gacgttataa ataatattga agatcaatgg tcagtccgtc gtctgatgca agccctaggt 54300 acggatttga ttgttaataa cttcgaccgc atgtattggg taaaattatt tgctttagat 54360 tatcttgata aatttaactc aggttatgat tattatatcg ttcctgatac tcgtcaagat 54420 catgaaatgg atgcggctag ggcgatgggt gctacagtaa ttcatgtagt tcgtcctggt 54480 caaaaatcca atgatacaca tattacagaa gctggattgc caattcgtga tggcgattta 54540 gtaattacaa acgatggttc tcttgaagaa cttttttcta aaattaaaaa tacactaaag 54600 gtactataat gtctgaacaa actattgaac aaaaactgtc tgctgaaatc gtaactctga 54660 agtctcgtat tcttgatacg caggaccaag cggctcgtct gatggaagaa tccaaaattc 54720 tgcaaggaac tttggctgaa atcgctcgtg cagtaggtat cactggcgat actatcaaag 54780 ttgaagaaat cgttgaagct gtcaagaatc ttactgctga atctactgat gaagcaaaag 54840 atgaagaata atggaattta aagacttttc aacgggtctt tatgtagcag ctaagttttc 54900 agaattaaca cttgatgcgc tggaagaact ccagcgctct ttacgtgttc ctaatccagt 54960 tcctagagaa aaaattcatt cgaccatatg ttattcaaga gtaaatgttc cgtatgttcc 55020 atcaagcgga agttttgaag tagcttcttc tggacattta gaagtatgga aaacacaaga 55080 tggatcgact cttgtacttg tgctagattc tgaatatctg cgctgtcgac acatgtatgc 55140 gcgggcacta ggtgctacac atgattttga tgattacaca ccgcatataa cattgtctta 55200 taatgttggg cccttatcat ttagcggtga tgtacaaatt ccggtcgtat tagatcgtga 55260 atacaaagag cctcttaaac tcgattgggc agatgattta aaataatttc acaaagttgt 55320 ttacatgctg atgaggtagt gatactatta tctcatcaaa attaattagg aaaataaaat 55380 gaaaactttc aaagagtttg ctacaaaaac tactattact gaatcttctc atggtatgga 55440 agtaaagctt ggaatggctt tagctgaagc tgagcgtctt ttctctcgta ttaaagaact 55500 tgctgctgca gtcgatcctt catcttttaa aggagaccaa actaaagtta aagcactttt 55560 agcattatgc tcagatgcag gagaaattgc taaaaatggt tctaagatga agaaacgatt 55620 agaagattta aaataatttc aaaaagttgt ttacacaggg ttttagtagt gatactatta 55680 ccctatcaac taccgaggag aataaaatga aacgttgtga attaattcga aatgttgcta 55740 ttgcaatttc tgcttccgct tttagttttt caatgtttgt tggatttata tgcggattat 55800 tgactacagc agaaaatgtt ttttcacttg tagtagcatt tttaattggt ttaatcgcta 55860 ttgttatgga taaaatttct aaaggtgaat aaatgaatat taacgaatat tatgtttatg 55920 ctggggatta tgcaaatcct tctcattttg aaggtaattt aatacctgat agagttttta 55980 atactccttt tgaagcgtgg agttgggttg aaagtaaaaa tggattttct tatcgttatg 56040 ttgaagtaac tgattggaaa ggaactaaat atcctaaaga gcattattat gtagacccat 56100 ctaaagtaaa ttttcttttg ttcgcaggcg ataattacta tccttgcggt ggatatgatg 56160 atttgattgc gtatgctgaa acagaagatg aattacgcca aattattgaa gacaataaat 56220 caaaacgatt tggcgatagt tttgactggt ggcaaatcgt aaatgcccat acacatacta 56280 ttgttgataa aggttaataa tgattcttta tgcgaaagta tcatccattg aaaatggata 56340 taaatatgat caagaagcag ctaaagcctt gattgatgat tatggcattt taacatgttt 56400 tgaagttgaa aaggtttaca ttgatcgttc atcttctcaa gttaaattag tgaaggaaga 56460 ccgtaaattt aatacagtaa attttgattt ctttattgaa acggaaaaag gtcctcttga 56520 atatgatatt ttcaagaatc ctttgggtct tgaatgcatc gtaaatatgt attatcataa 56580 atggtaaata tgctttaaga attatttgtt attattaact catatcgcac tgattaatac 56640 cctctatcat caagggttct tgcttgagag cctttgttaa taattgggaa tcatattatg 56700 gatactaaat tagctgaaat ttttgaagga aaacatccaa attttagtac aggaaagctg 56760 aaagataaaa taataaaaga aaatattttt ccatatgagt gcgcaatttg taaaatttca 56820 aaatggcaaa ataagcatat tgttctgcaa ttagaccata ttgatggaaa taacaaaaat 56880 catcttaaat caaatttaag attattatgt ccaaattgcc attcgcaaac tgatactttt 56940 gctggtaaaa atattaaaaa taataaaaag cgttataatg ttagtgatga agaaatttta 57000 aacgcactat taaattcggc tactatatca tctgcaatta cttcattagg tttaagtaaa 57060 ggcggaaact ataaaagatt cattaaaatt gctaatgata ataaattata tcatttactg 57120 gtaaatatta atgaaaagat tttaactgat gaaatttacg aagaattagt taaaatcgat 57180 tattctaaat ttggatgggt tagtaaagct gctaaaataa taggtataag cccacaaaaa 57240 gctagaaaat ggatagaacg aaagtgtcca gaattgatag aacaatcttt ttcacgtaat 57300 aaataatata gagcgagaat ggtcaaattg gtaaaggcac agcacttaaa atgctgcgga 57360 atgatttcct tgtgggttcg agtcccactt ctcgcaccaa atttgcggat atcgtataat 57420 ggcattacct cagacttcca atctgatgat gtgagttcga ttctcattat ccgctccaat 57480 ttaatttact ccgtgtagct cagtttggta gagcgcctga tttgggatca ggaggtccaa 57540 ggttcaaatc cttgtatgga gactggaggc gtggcagagt ggtttaatgc accggtcttg 57600 aaaaccggca gtcgctccgg cgactcatag gttcaaatcc tatcgcctcc gccagttttg 57660 ctgatttagc tcagtaggta gagcaactca cttgtaatga gaaggtcggc ggttcgattc 57720 cgtcaatcag caccaaggcc ctgtagctgg aaggttcaag caagcgactc ataatcgcca 57780 gatggtggtt caattccacc cagggccacc aaattaattt ggggagttat cccgtagagg 57840 tagcggtgtg gactataaat ccattgtcat tgcgactcgg gtggttcgac tccatcactc 57900 cccaccaatt tggatgtgta gctcaatggc agagcgatcg cctgttaagc gattggttat 57960 aggttcgaat cctatcacgt ccgccaaatc cgaggcatag ctcagaagga agagcaagga 58020 ccttctaagt cctaggtcgt aggttcgatc cctactgcct cgaccaaaca agaaaccggg 58080 tcgctaccgg taagtcgtcg gactgatgtt ccctgagtaa ggactgacat tatcaaatct 58140 ttacgatctg ttaatgtccc cttacatcac agcagaaacg gcgcacagaa ttatcgattc 58200 gaggaaatat ctttgccgta agccgagtag cgtttttgac ggaacgttcg gatatggtcg 58260 agatatggcc ttttaaaaat attgagtagc gtcaactgct taataaccgg gttcgaatcc 58320 cggcgtttcg tacaaacact tgccttagca ggtggaaccc cgacaaggtt gccgcaaggc 58380 ttagccccga ccgaaaggtt ggggcttttt agtataaata tagtagtata ttaaatccac 58440 gaattaaaac aggaaataag atgaaatcat atgctcaatt tttaaatgaa gcggtgttaa 58500 atgaagcatc tagcaccgaa attcaagctg tcgcaaaagc agccattgca gcgggtaaat 58560 attcctataa agatgcttct gacgaatcac gattccaatt tgctcgcgac atgaaagcag 58620 aaggatttac gggaaatgca gttagtatgg cctggaaaag tttagttgct actggcgctg 58680 cttttgcaaa gccttcgggt aaacctgctc ctaaagcaga tcctaaagcg gcacaagaaa 58740 aaaatatcgt taaaggaatt atctctaaat atgaagctat ccttaaagag cttttagtaa 58800 tcaaaaccga aggccaaaag ttagcccgtg cttatagttt caaagataat ccacatgttc 58860 actctcttga gtatgttgaa gacatccaaa aaattattaa agaccgcatt tggtctgcta 58920 aacaaatcaa ataacattct tagccccgac tgaaaggttg gggcttttta gtttgaatca 58980 catagagaat gctgctacgg atagtaacaa agtaataaat aattaataac caaccgataa 59040 attatttcaa ggattttaaa tgaaaaccta tgccgaattt ttaaccgaag cagcaaaatt 59100 accatctgaa gcagatctta ctaaagtatt cttccaattg gacccaaaag accgcggcga 59160 ttttcttaag tggaaagcta aagctattga aatgtacaac atcgataata gttcttttac 59220 aatgagtcaa gaaaataaat tcaataaagc atttttcaaa atttctaaga aattggcatc 59280 tggcgctcag gttcctaaat ctgtattagc tactcctgaa cgtgcacctg ttaaaatttc 59340 taagaatatg ttcgacacta aaaaatacgt taatgctttg aataaagctc ttgatgcatt 59400 ggatgatgca aagaaggcag cccgcgatct tcaagacgtg tacaccgatt ttgatcgtaa 59460 aactaaaggt tctatttcaa atagcgaacg caatagtgta agtgtttatt ctgatagcct 59520 tgatgttctt ggcgatgcgt atactgaaat taaaaatcgt attaacaccg catctaagct 59580 aaaagctgct gccgaagcta taataactaa actaggcaaa taattttaaa tccctatcta 59640 aatgataggg ctttttggta tctaggcctt tctggacctc tctaggcatc atttagttta 59700 taccctttat aatatattat cctatctttt aattgcccat ccctgcccta gaattcccta 59760 aaaattttta aaattttttc acaaaactat ttacatccct gttcttccat ggtactatac 59820 aactatcaac tactgataca gaaaaacaac ttggagaatg aaatggataa ttacggcgaa 59880 ctgttcaact tctttatgaa atgcgtttca gaagatttcg gtcgtacagt gaatgatatt 59940 aaagttatcg gtcctgacca tccgatgttt gaaacttacg cagtaatggg taatgaagac 60000 ggacagtggt atactgtaaa ggttgtgatt aacatgttca cagcagaagg ttatgttaaa 60060 ctgtcttcta aagtttacca cgataacgac gaaatcgcag aagaatattt caataatatg 60120 aaataagttt acataggctc atgattgaga tattatgagc ctacaatttg aggaaaaccg 60180 ctatgtctct ttataccgaa ctgaatgaaa cttccgaaaa atcttttgat gatatcttat 60240 caataatgca aaaacaggta ttagaacagc ttagtgaagc cgcaagcaat gggcacaagg 60300 cttgtcgagt ccaaagccca catcgccgca tgtatgaacg ccctcttatt aattggctag 60360 aaagcgaagg tcttacagtt aagatttgcc ctgctaaatt tgatgtccca cgtattctta 60420 atatttactg ggataaaccg gctgagaaat tggttgagaa aagtgaccct tgggttgtta 60480 atgtaaaaac atgtgacagt ggatgctcac ttgaggtgtc tgctgtaatt aaaggcactc 60540 atggtgaaaa atcatggggt tggcctagta aagataaaga aatcgtgtta agtgtaaaag 60600 ctactagtat tagtactcct atagctaaaa gcattatcgc cgccgccgtt gaagaagctg 60660 aacgaatttg taaaattaaa aatagtttac atctcttaaa tagtatgata taattcttcc 60720 acagatttta tttttaatca acctaaggaa atactatgaa cacactgaag aaaattgttg 60780 aatttattcg cactaaactt ggttctgcta tggctaaaaa tttatctgtt gaagaacagt 60840 atactgctgc agcagcaaaa ctacttgata aaattaaaga tctaaaaact gcttctgtta 60900 aatctattaa tgaagaaaaa cgtattcgtg aacttattgt cgaaaagaat cgacaagccg 60960 aatcaaaaga gcgtgaaatt cgtaaacttc tttccgaagg tcaagatgta acaatgcatg 61020 ctaaactcgg tttactatat cgtcgaacag ctgagcagtt gactactaag gctgacggtt 61080 atgctgaaat gcgaattgaa atcgccaaga aagtagttga gttagatgat gctcgtcaag 61140 aacttgcggt taaattggaa tatatccgtg aaactcgtgc agcaaatgcc cttggaatta 61200 gtactgccga tgatgtagtt gaaattgcag cactgactaa ggttgatatt gaagataccc 61260 ttgctcgagt tgaaaccttt aacggtaata tttctggggt tgaaactacc tctgccgatg 61320 ttcaggaata cattaattct ctgaaataat gataaggggc ttcggcccct tatacttgga 61380 gtaaatagga atgaaaatga aaatgcaaag tgatttcaat tcaatgtttg aagagttcca 61440 aagacaggtt gatgttccag accaattact aaatgctctt aaacgcatgg cagaaggacg 61500 taattattat tgggggtctt catacgaaac tgatgaaagc ctttccggaa gattttctag 61560 aggtaaaaag tctttaatac gtcctggaat actcattaac agtattgaat caattcattc 61620 attgacgtgt gattttgatg ttgaatttac tgatttcatt tctcctgaat ggacggtttg 61680 ttatttaaac gacgattatg attatcttgg cgtttatagt ttaagtgacg catggtttaa 61740 acgtaattta caaaagtcaa atttattcta tattgatact acggtaaaat ttcagggaaa 61800 gaaatatttc tttactctta tagttgattc tgaaacaaag catgaaaata aacgtattct 61860 tagtaaaaag aatatcttaa ctattgttga tgatcttttt gataaattag tggaaaatcc 61920 taattttgaa agcgatttat tattagaaaa atttgttaag gaatgtagag aatatgtcaa 61980 agccatcact ataccttcca agtaaacctg tgaagtatga accaaagcgt cagctaattt 62040 ctactgatgt gttaataggt cctgtggtag ctgtatcatt tgtgattttg ctaattgctg 62100 gaatggttat agacgttatg actgatattg aatctggtgt aatatttgga gcaacactaa 62160 ttcttccatt ggtagttccg tttttattag tacctgtaaa ttgggtagga tactggtatc 62220 aaggaagaca ctatcgtaaa cgtgtacgtg attggaaagc ccagtgcaaa aagattaaaa 62280 aagaacatca gcttaaactt gctgcgtatg aatttgatga aattatgaaa tttgttaagg 62340 aatcacgatg caaaagccaa aactaaataa agtcaaatat tctttttctg agtcattttt 62400 aatttttgct gtggcgttgg ctgcagctct tgcgggtagt cttattggat tgttaattga 62460 ctgttttatt ttaaacatcc gcagtaatgt cagcggtata gtagatataa tagaagtttg 62520 gagcgaactt cattatacta tagtaatttc attattttca ttctttggta ttattttata 62580 ttttcattat gataatttta aaataaattg gcaaagaaaa aaggattaca aaatacaatt 62640 aaaagaatat aataactaca tgtcttatat tgaaaatgaa tcaatgaaag agtttgtgag 62700 tgattgtagg aaaatcaaat gattttaaaa actcgctggt atgatttaga tgatggggac 62760 gatggcattt cagttgatag agttgactgg agcggctgtt ctgaagatac aaagaaacga 62820 ttaattaggg agtttagaat gggatatcaa gcagctaagc catctactgt aacagatgat 62880 aagttcgtat gtattcaaaa cggtcgtgct aagttaacga acgctgattg gtttacaggc 62940 aagaatatgc ttttatggta tatcattagt cttcctgtgt catcattcgt attttatttc 63000 tttggaaaaa atccaatgga tagaatagga gattgggctc tttttactct gcttgttaat 63060 atttttataa cagcagtaat atcaggaata tggtgcgtgt tcattgaaat gccatggcgg 63120 ttacgtaggc agcaaaagat ttttgatgaa aagaaatata cccaaaattt aaataacttt 63180 atcactgaat gcaggaaatt aaaatgaaaa cactatcagc tggtattatc tttatgacag 63240 aagataaaga tttatttatg ggacgagtta ctggttctcg taaacctgga atgatggcac 63300 atcgctggga tattccaaag ggccgtgtag aaagttctga tttgaatgca ctggaagctg 63360 caaaaagaga atgctcagaa gagactggtt ttatcgatta taattcagac cttctagaag 63420 acctaggtgt atttaaatat tctagtaata aagacttaca gttattttat tatacgattc 63480 cagtagagca tgagatgttt agaaattgcc attgcgagtc ttattttgaa aataaagatg 63540 gtgttatgat tccagagatg gatgcttttg cccttattcc tcgtactcag tggcaatatg 63600 tgatgggtcc ttcactttac cgaataatga acagcctctt ttaatttata aataccttct 63660 ataaatactt aggaggtatt atgaatatat ttgaaatgtt acgtatagat gaaggtctta 63720 gacttaaaat ctataaagac acagaaggtt attacactat tggcattggt catttgctta 63780 ctaaaagtcc atcactaagt gttgctaaat ctgaattaga taaagctatt gggcgtaatt 63840 gcaatggtgt aattacaaaa gatgaggctg aaaaactctt taatcaggat gttgatgctg 63900 ctgttcgcgg aattctgaga aatgctaaat taaaaccagt ttatgattct cttgatgctg 63960 ttcgccgctg tgcattgatt aacatggtct tccaaatggg ggaaaccggc gtagcaggat 64020 ttactaattc tttacgcatg ctccagcaaa aacgctggga tgaagcagca gttaacttag 64080 ctaaaagtag atggtataat caaacaccta accgtgcaaa acgagttatt gcaacgttta 64140 gaaccggaac ttgggacgcg tataaaaatc tataaagttg tttactttct cctagaattg 64200 tgatagtata ttaacagtta cttggaggga taaaatgact cgtattaatt tgactttagt 64260 atctgaactt gctgatcaac atttaatcgc agaataccgt gaactgccgc gtgtttttgg 64320 tatagttcgt aaacatgttg caaatggtaa gcgggttaaa gattttaaaa tatcttccga 64380 gtttatttta ggttctggtc atgtcacgtt cttttacgat aagttagaat ttttgcgaaa 64440 gcgtcaatca gacattataa cggaatgctt aaaacgtggg tttagtataa aagatactga 64500 agttcctgac atcagcgata ttccagcaga atggaaaaat gattataatc catgcaaatc 64560 agctattaag ttgagtcaac aacgactcga tgaaaaaatt ttaatgaagc catactggta 64620 taaatactac ggtaaaacta tttacattta aaggaaacac atgaaaacat atcaagaatt 64680 tatcactgaa gcagctatta attctcaaat tatcgctgaa tcttttactg atcttttgaa 64740 atttaaaaaa ggtcaaaaaa tcactgctgt attggatgat ggtacagaag ttgagatgga 64800 tgtacaggga tataattatg cagtagatgg aaaactgtat aataaatctc atgctaaatt 64860 tgattcattt gacgattttg ttaatacagt tgaagatgaa aaaactcgta gatccattgc 64920 aactggcgac gctaaggttc ttatggcaca cggtcatgaa cgtatccgcg ctaagcagaa 64980 taaaatgggt gaagataatt ttgcattagt tggttatcaa tctggtaaac aaacttatgg 65040 ttatcagcgt actgctacca tgtataacaa aaatggtaaa atcgctttcg tgaatagcaa 65100 aggctctatt cagtacgtca aatcattcaa ataagcaaat aagaaatatg ggaacaagct 65160 ggacctcatg attctatgag ggattcccgc caacctgtaa taaggtcgag cccaagtgcg 65220 gtaatgggta aatacagaaa tggacaattc atgcgccatg gaatggccca aatttagaga 65280 gaataaaatg agaacatttt taactggtcc ttatctatcc ctgatgaatg cttttacaca 65340 ccattctgat gctagagtag aagaaatttg taaaaacgat tatattccac catttgaaga 65400 cttgcttaaa caatattgta cacttcgact agatggtgga cgccaatctg gtaaatcaac 65460 tgctgtgact aactttgctg ctaattggct gtatgacggt ggaacagtta ttgttctttc 65520 taatacttca gcttacgcta aaatttctgc aggccacatt aaaaaagaat tttctcgtta 65580 ttctagtgat gatatacgtt ttcgtttatt tactgattct gtccgtagtt ttatcggtaa 65640 tagaggcagc aagttcagag gtttatcgct ttcgcggatt ttgtatataa tcgatgagcc 65700 tgttaaatct cccgatatgg ataaaattta cgatattcat attgaaactg tacaatactg 65760 ttgcaatagt aaatgttgca ccggtggtat tactcgtcct caattttttg tgatcggaat 65820 gcaatgatga cagacacaca gcttttcgaa tatctttatt tttcgccaaa aactattaaa 65880 aataaattgg tgaatcattt tgaaattttg gcaaataata atgtcttgag cgaattttat 65940 cctaagcaat acaaattaca aaaaggtgta ttcaaaggat gcagagtttt gtgtactgct 66000 cctaatgcaa gattaatgaa taaaattcca tattttacca tggaatttat tgatggacct 66060 tttaaaggac taatcaccca aagtttaatg gcatatgatt ctgagccatt tttaattaaa 66120 gaacagtctt ggataaattt attttctaat tgaggttata tgaaagcata tcaaattctt 66180 gaaggcacac ataaaggtac tatttatttt gaagatggta ttcaagcacg aattattgtt 66240 tctaaaacct ttaaagagga ctcttttgta gacccagaaa ttttctacgg tttgcacacc 66300 cgtgaaattg aaattgaatc acatcctaca gttaaaattg aaggtggtca acacctgaac 66360 gttaacgttc tgcgtcgtga aactctggaa gatgcagtta agcatccgga aaaatatccg 66420 cagctgacca tccgtgtatc cggttatgca gttcgcttta actctctgac tccggaacag 66480 cagcgcgacg ttatcgctcg tacctttact gagagcttgt aatggcaaag ataattattg 66540 aaggttctaa agatgtgtta aatgctttcg ccgagtggtt tagtaattca ggcgaacagc 66600 aatttaatga agcatggacc atgggtgata ttgatggaat ttatcctacg acagaaattt 66660 ctgttcaagg atatggcatt catgaaccta ttcgtttagt tgaatatgat ttatgtactg 66720 gtgaggaagt caaatatgat tgaagatatt aaaggttata agccacatac tgaagagaaa 66780 atcggtaaag tgaatgctat caaagatgct gaagttcgtt taggacttat ctttgacgct 66840 ttatacgatg aattttggga agcactagat aattgtgaag actgtgaatt cgcgaagaat 66900 tatgctgaaa gcctcgatca gttaactatt gctaaaacga aactcaaaga agccagtatg 66960 tgggcttgtc gtgctgtgtt ccaaccagag gaaaaatatt aatggctcaa ttaagcgcag 67020 ggtttggtta tgagtattat actgcccctc gtcgtgtatc tgttgctcct aagaaaattc 67080 aaagtcttga tgacttccaa gaagtagtcc gtaaagcttt ccaggactat gcgcgttatc 67140 ttaaagaaga ttcacaggac tgtcttgaag aagatgaaat tgcttactat gagcaacgtc 67200 ttgaacagct taaaaacctg catgaggtcc gtgcagaagt ttcaaagtct atgaataaat 67260 tgattagatt taaagaataa ctgtttactt ttcctcttga ctgtggtata atttttctat 67320 cagttaagag gagaataaca tgactatcaa cacagaagtt tttatccgtc gaaataagct 67380 tcgtcgtcac tttgagtcgg agtttcgtca aattaacaat gagattcgtg aggcatcaaa 67440 agcagcaggg gtctcatcgt ttcatctaaa atattctcag catcttcttg atcgtgcaat 67500 tcaacgggag attgatgaga catatgtttt tgaattattc cacaaaataa aagaccatgt 67560 tttagaagtt aatgaattcc tgagtatgcc tccgcgtcct gacattgacg aggattttat 67620 tgatggggtt gaataccgtc ctggacgttt agaaattaca gatggaaatc tttggcttgg 67680 atttacagtt tgtaaaccta atgcgaagtt caaagacccg tcgcttcaat gcaggatggc 67740 aattatcaac agtcgtcgtt taccaggaaa ggcttctaaa gcagtaatta aaactcaatg 67800 aggtaagcat gagaaaagca ctactcgctg gtctattggc catttcaatg atggcgcata 67860 gctccgagca tactttcagt aatgtccaac tcgataacat gcgttacgca tatcaattcg 67920 gggaacaatt ttctaaggat ggaaaatata aaacacacaa aaatatccac aagagcggat 67980 tgggtcatat aatggctgct attttgtggc aagaaagctc tgccggagtt aatttaaaat 68040 ctaaaccaaa gcatcatgcc tatggaatgt tccaaaatta tttgccaact atgcgagcaa 68100 gagttaagga acttggttat aatatgaccg atgctgaaat aaaaagaatg ttgaataaac 68160 ggtccaattc agcttcctgg gcgtacattg aactttctta ttggttaaat atacataagg 68220 gcgatataag aaaagcaata tcctcttata attcgggatg gaatgttaaa gcaggttcta 68280 aatatgcttc tgaagtccta gaaaaggcta attaccttaa aaataataaa cttttggaaa 68340 tagtaaatga ctaaaatttt ggttttatgt ataggattaa tttcattttc tgcttctgca 68400 tcagcagata catcatatac tgaaattaga gagtatgtaa atcgtactgc cgcggattat 68460 tgcgggaaaa ataaagcatg ccaagctgaa tttgcacaga aattaatata tgcatataaa 68520 gacggagaaa gagataaatc aagcagatac aaaaatgata cattgttaaa acgatatgct 68580 aaaaagtgga ataccttaga atgttcagtt gcggaggaga aagataaagc cgcttgtcat 68640 tcaatggttg accgtttagt agattcttat aatcgaggat tgagtactag atgattgtaa 68700 aatatatcaa gggcgatatt gtcgccctat ttcttcaagg taatattatt gcgcacgggt 68760 gcaattgctt ccacacaatg ggctctggcg tagcaggtca attagcaaga gcctatccca 68820 aaattttaga aatagataaa accactaccg agtacggttc tcgtgataaa ttaggcgata 68880 tgtctattgt ttttaaacat aatcctacag gatttggtat atgctataac ctgtatacgc 68940 aatatgaacc gggtcctaat cttgattatg gtgctttagt aaactgcatg atagaattaa 69000 atctacaggc agaaaccctt ttgtttaaac cagtaattta cattccacgc ataggttgcg 69060 gtattgctgg tggtgattgg gataaggttt ctaaattaat tgacatgttt actcctgata 69120 ttgatttaat agtggtggat tatgaaagta cattacccgc atccgtttga tcctaaaaac 69180 aaagtggaaa ttattcgtca atgggaacgc atttgccgta ctaaatgccc aattaatagt 69240 ccacatgatg tagataaaga ctatattgga acattcgttg aatatacctt tattgataag 69300 aaaggtcgta aacaacatgt agaagaatat tgcttaaagg ttacatggtt atgagccaaa 69360 ctagtattct taaaaatgcc cactgcgaaa agtgtgaatg gccggttgtt tttgctttat 69420 gtaatgatga aatggcttgc gatttcgatt attggtgcta ttgttctaat aaaggatgca 69480 tcaatcataa aggtgaagga ttttattcag gattttatcc ttatcctgat ttcgttaaag 69540 aaggtaaacc aaaatgaacg atgatttaaa atatcaatta ttacgtgaac ttgatgtttt 69600 gattgaactt tctgcgcaaa aaggatttat aattggatca ggtcaaaaag accccaacgg 69660 tcattcaatc gtagcggtta tgaatcagaa acgagtcatt ttaaaacttt tggggattga 69720 catactgtga gcctaagcaa agaacaaaaa gacagactat tttctcttat tcacgaagtt 69780 atggataaaa atagcgaatt ggaaaaagtt tgtgatgaat gcggtccttt taataccgac 69840 gaatacgaag aactgtgtaa agatttcgac gataaagaac aagaacttat taattatata 69900 aattccttat gattactcgc gaacaaaaga acgaaatatt atttttagtt ggtgaaatta 69960 ttagtttaga aaaggatttg tcttttgaaa tatcttctga atacggagat gccgaaacat 70020 attacgaatt agtaaaatct atcgataaag ctgaaaatga tttagaaaca tatttagaaa 70080 atttaactaa ggactaagat ggcgagttta atttttactt atgcagcaat gaatgctgga 70140 aaatctgctt ctcttttgac tgctgcacac aattataaag aacgcggaat gggtgtatta 70200 gttcttaagc ctgctattga tactcgcgat tctgtctgtg aagtcgtttc tcgcattgga 70260 attaaacagg aagcgaatat tattacggat gatatggata tttttgagtt ctataaatgg 70320 gcagaagcac aaaaagatat tcattgtgta tttgtagatg aagcccagtt tttaaaaact 70380 gaacaggtgc atcaattaag ccgaattgtt gatacatata atgttcctgt tatggcttat 70440 ggtctaagga ctgatttcgc tggaaaatta tttgaaggtt ctaaagaact tttggcgatt 70500 gcagataaac ttattgaact aaaagcagtt tgtcattgtg gtaaaaaagc tattatgaca 70560 gctcgattaa tggaagatgg aacaccagtt aaagaaggta atcaaatctg tattggtgat 70620 gaaatttatg tttctttgtg tagaaaacac tggaatgaat taactaaaaa gctcggttag 70680 tgcaaaagtt ataaataggt ttatctaact aaaggggtat atatgctaca attaactgaa 70740 aagcaacttc gcaatcttac tgttcttcaa ttagatgaaa ttcgtaggga agttggaaat 70800 atcatttcag ctttgcgtcg agaagtatca ctcaaccaat ctccggcaga ctatactaga 70860 ttgcgaaatt ttgaaaaata ccttgataaa gttaaggccg tgcatcggca taaagtaaat 70920 acaggacaaa aatgatagga ggcctttatg gccttaaaag caacggcact atttgccatg 70980 ctaggattag cgtttgtttt atctccaccg attgaagcga atgtcgatcc tcattttgat 71040 aaatttatgg aatctggtat tagacatgtt tatatgcttt ttgaaaataa aagcgtagaa 71100 tcatctgaac agttctatag ttttatgcga acgacttata aaaatgaccc gtgctcttcc 71160 gattttgaat gtatagagcg aggcgcggag atggcacaat catacgctag aattatgaac 71220 attaaattgg agactgaatg aaattcagcg acttttcaca aagtggaaaa ccttcaaagg 71280 cagatgaata cttaggttta ttaatggctg cacaagctta ttttcattct gcacattttg 71340 aaactaaaag ttatgctaga cacaaagcat acgattttat tttctctgag ttgccagatt 71400 tgattgataa atttggtgag caatatttgg ggtattctgg tagaaaatac acgccttcta 71460 ttccagatgc cagtaaactt cctaccgaca caattaaaat gattgatcgc atactagacc 71520 aatctaacag catttataaa gaaatgcctc cagccattca aagcacgata gatgatatta 71580 ctgggatgtt ttaccagagt aaatatcttc tttccctcga ataacattag tctccttcgg 71640 gagacttttt tcattttacc ggtttacttt ccatttgagc tgtgatacta tacaactatc 71700 ggataaagag gagaacatca tgaaaattga agcactcaat caagaaggaa atatctacgt 71760 catcattaat ggtgattttt tcgtcaacat ggatgaagtt actagtgaag aacttgtaga 71820 acttcttaag aaacgttatg atatgtgtga tgaagctgca actcacatgg cgtgtgcaat 71880 attctctctt tcatatgtgg tggaataatg attagcattg aacaagcaga taaaattaaa 71940 gaattggtag ctttaattcg caaagcagat gaggaactta gtgactttgc ttggttttcg 72000 tcaggcattg caaataaagg tattgaaaaa tttgaagcta aagttgataa tgctgtagaa 72060 gctttagata tgtttcttga tgaaattatc gatcataata cgagagttta agtatgctaa 72120 cacgtgaaca gtttgaaaaa atcattaaat tagcacgtga tattgaaata gattcatatc 72180 agttggcagt tgagcattgt gaaggatact catacgatgg tatagaagca gctaaaaagg 72240 atttggataa atccaaagct aagttagttc aatatcttga aatgattagg tggaataatg 72300 aaaactgaaa agcagatgtt tttaatgaag ctaattgaag aatatgctaa tgcagtttct 72360 gactatgagt attctgctcg agaaagaggt acagctttcg caaaggaaga aatgaaaatt 72420 atggttgatg ctcacacaaa gcttcagaat tttattgaaa acgtcattta atggtttaca 72480 agttggcaag gttatgatat agtaatcttg tcaactgcca aggagaagag aatgaaagtt 72540 ttgtttgttg tgtatgtgat gattcaatat aattacccaa tgtttactta taatctggtg 72600 aacaatatta ttgatattat tcaaaggagt atgtaatgac aagtgaacaa gcttttaagt 72660 taagagaatt aattgaaact ttaattggtg aataatgtga gctttccgaa attagaagta 72720 ggtgatttag tcttaacaaa attgtggaat gacgctcaat cagtagaaat ctgtcaatat 72780 cgtggagcaa caggtaattt gatgtacacg atttataacc cagaaatttt gttagagtgt 72840 catttggaac gttttataaa agacaccgat agtatgcctt atagtgtatc gattgtacgt 72900 aaatctgata caaaggaata ctctaaaatt ttaaaacaaa ttcatgccaa taaaaaggat 72960 taatatgaaa cgattagtac tagaagttag ttcacttttt ggtgaattag ctatagaaaa 73020 agtaaataat atgtatcgtt tgacgcaaga agatgatatg atgtatttta cgcctagtga 73080 aatcattcat ttaacccaaa ttgaaaatcc ttatactgat aaaattgtaa gcattaatga 73140 tgagcataaa attcattttt attcttcatg cccaggattt aatattgaaa gcgagtcaat 73200 atgcctatca gttattcatt gggacagttt tatagataag attaaatatt tttattattc 73260 taatgaaaga aaacatagtt taaaatggtt taaaaattgc aatgctatta ttactaacgc 73320 ttgcaatcag aatgatgaaa ctgttttaaa cgtatcaaaa tgttatgaag atggagatgt 73380 cttaactatt cgtcaaattg atgattttcg atcgcatatt gtcacattta ccaaagacga 73440 agctattgcg ctaaagactt atcttgattc tgtcattcca actatgattt caaagtgagg 73500 aaatatgttt atttcaagtg gaagtggttt aattcgcgtt gaatttaaaa atgacatctt 73560 ccttagtcaa ggagatgata ttattaaaat gagttatgat gaaattaaga aaatttgtca 73620 cattcttgaa agtcgtggaa aaggaaatgc tactatcgat ataggtgatt tatgggtgac 73680 actttatgaa gtatccgaag gatttaacat tgaagatgaa aacaacatct tagctattga 73740 taaaagaagc gatttgtttg atgtgttaaa agtttatgaa cagtcaaacg gtggaagaaa 73800 agctgcattg atttatcaaa aaccacattc atgcggaact gcttcaatta tttcagatat 73860 tgaagatgaa actgatactt atatgtgtgt tttaaaagct ggtggtgacc gtcatccgga 73920 ttttatttct attcgtcaaa acaatggaga aatttcatta tcaaaatcag aagctgaagc 73980 tatgattaag tatttaacaa ctgttacacc ttcaatgaaa ggataattat gattattaat 74040 gaaaactctt ggcactataa attattcaaa ctgtttaatg atgaatggaa acgacctaag 74100 acactgtgtg catacttttg gtctattgtt atccctacat ttttcgtttc ttttttcgga 74160 tgtactatac tcgcaggtct aactattatc tgtgcagaaa tcatgcagaa atggcttatt 74220 tttggtagtt tatggactct tattccatca gcatttatac ttgccatttt gcttgtttta 74280 cttattattg gttcatttgt tattcctgca caactgcatg aaaaatataa agattataaa 74340 tggaaaaagg attatgcttt acatgtagaa aatattgata gggcgtataa aggtttacct 74400 cctattcaac ccaagaaatc tattatcgtc gaatttttaa aagcgcgtaa agctaaagta 74460 tgtcctgtta ttgaatataa ggctgaatga tgaaaacagt aatgaaaagc tattttggta 74520 gtcatcttta tggaacttct actccagaat ctgatgtaga ttttaaagaa atctttgttc 74580 ctcctgctcg cgatattctt attggaaatg ttaaagagca tatgagtaaa aacactaaca 74640 acacatcatc taaaaacact aaagatgata ttgaccatga actatacagt cttaagtatt 74700 tctttaaatt agcagcagat ggtgaaactg tagcattgga tatgctccac actccaccgg 74760 aactagtagt taaatctgat ttgcctgatg tatggaagtt tattcaagac aaccgttctc 74820 gtttttatac gaccaacatg aaatcctact taggatatgt tcgcaagcaa gcttctaaat 74880 acggtgttaa gggttctcgt ttggccgcat tacgtgatgt gttgaaagta gttaatcaaa 74940 tccctgagca atgggttgat taccaagaag acggttccat taagcagcgt cgaactaaag 75000 ttgaagatat taagcatcgt cttccagaga acgaattctg tgaatgggta ttccataatc 75060 atgagaaaac aggtccacaa acgttctaca ctgtgttggg tcgtaaatat caaacaacgc 75120 tttctcttat tgagcttaag cagtctttga ataaacttga tgctgaatac ggcgaacgtg 75180 ctcgtaaggc tgaagcgaat gagggcattg actggaaagc tctgagtcat gcttgccgtg 75240 gtggactcca actattggaa atttacaaaa ctggtgactt agtctatcca cttcaagacg 75300 ctccatttat tctcgacgtg aagttgggta aacatccatt taaaactgtt caagagtttt 75360 tggaagatgt tgtcgatcaa gtagaagcag catccattga agcttctaag aacggtatgc 75420 agcaaaaagt agacatgggt ttctgggatg acttccttga gagggtttat cttgaaaacc 75480 accgaagtta ttataaatga tagggagcct tcgggctccc ttttttattt caaaattttt 75540 tcacaaaaca gtttacaagc ataaagcttt atggtactat acaactatca actgatacgg 75600 atttggagaa taaaatgaaa actgtaacta tcaataaggg tatctacttc ggtaaagaaa 75660 tctctggaac ttttgagctc ttaggcgaat ggttcccaga taatgctccg gtagatgcac 75720 aaggagatgg taaagttttt gttgaaattg acggtaaacg tcgcggtgtt tgggtttaca 75780 aatcagatat ttcatatgat ggtgtaaaag ttgaagaagt taaagaatca tatgaagata 75840 tgaaaacccg cattaataaa agatttaatg ttatgggaat gatgacgaat ggtattatta 75900 acggaaatat tcgttcatta attatctctg gcgcagcagg tattggtaaa acgtattctt 75960 tagataaagc tttaaataaa gcaaatgata ttggatacat tgaatataaa agcattaatg 76020 gtaaaatctc tggtattggt ctttatgagc agctttggaa taatcgtgaa gagaattctg 76080 tccttttgat tgatgatgta gatgttttct ctgatatgga cattcttaat cttctaaaag 76140 ccgctctgga cactggagag acccgtaaag tctgctggag cactgcgtct tcttacttag 76200 aagaaaaagg cattgagcgt gagtttgaat ttaaaggaac gattgttttt atcacaaacg 76260 ttgacattga ccgcgaatta gaccgtggta ctaaacttgc tccacattta caagcattag 76320 tgtcccgctc ggtttattta gatttgggtg ttcacactaa tgaagaaatt atggtcaggg 76380 ttgaagatgt tattctttca actgacatga tgcaaaagcg cggtctttct gatgaagaaa 76440 cttataaagc attatcatgg atgaaagtta atgttaatcg tttacgcaat gtttcactgc 76500 gtactgctct ttatcttgct gactttatta tgaccgacaa aaacggttgg gaagaaattg 76560 ctgaggttac tcttttgaaa taattcataa gaggacttct atgacaaaaa ggcagttcag 76620 aaatagatta tatggactgc cattaaaaag atgactagaa ttaaactgat gaatggaggt 76680 agtgatgtta tactcaaagg ctcgtgaaat ttacgaaact aaaattaaag aagcagtatt 76740 tcagttcgca acaacaatgc gatggacaaa tgactgggag tattcaaaaa atcataagaa 76800 gcccatggtg acaagaaagg ctcatatgtt agtgttaata gaccgtgagc agattaaagc 76860 ccgagaagcc ctccagaatc ataaaaaggc tgcctttgaa tggtttatgg ataacactgc 76920 tcctgagacc aagaaagcag tgagcgcctg gttcagtgga aaaaattgtg aaagaagtta 76980 cttttagtgg tttacaagac tgttcctctg tggtactata caactatcaa ctacggagga 77040 acagaaaatg aacgctaaag atattttcaa cttggtaaat tacaacgatg gtaaatttaa 77100 atctgaagca caaagcaagt tctttaatga catctcaatc ggaggtgaaa tcactgttga 77160 tggaggacaa atttacaaat cccgttggaa ttggatcgtt attattgatg agattggtat 77220 tgtagaaatt tataagaata cgaataaaaa tcgtacatta cactggtctc gtgatactaa 77280 cgaacagtac aaaaaggata aagcatctaa attatctcgt gtaactcaag aagatattga 77340 gttcatcaag aaagatattt taatgtatga taacttaatt gctgaagagc aagctgttat 77400 tgataaattt gacgagatta aagcttctcg tcaaattcct gattttatga aagaatcagt 77460 aaatgaacga tacactctca tttcagagcg tattgaaact tacaaaaagc aaagagctga 77520 acgccaaaat actcttcgga agtttgaaga acggttaaag acggtactcg cataaccgct 77580 ttataccaag gatggtataa tggttctaag cccttttaat tgagattatt atgaaacagt 77640 tgataattaa aagattgaat ttattgatat gttgtttatg tgtagtaatt gcatatggtt 77700 attacgcaat taatgattat atgcattata aagattatga tgttactgta gttaatacca 77760 ttacaggaac acaaggaaaa gggtctagtt tatcgtttat tgccgtatat gaactcaaag 77820 acggttatag atttagtgaa tatatttccc cagagatgta ttcttcaata gaaaaaggtg 77880 ataatattac tgtaagttta cgtcctttcg acgtaaaaca gacatggttt gataatatta 77940 tttggttctt tggaatggca ttagttcagt ctatatgcgg tacttatata gtctgttcaa 78000 tcttatttcg cgtaattagt aaaattgagt gaggaaaata tgtcagtagt aattaataat 78060 gtcaatgcag taattaaatc tttagttaat aaaaaaaatg atgaatgaat ggactgtatt 78120 tcgccgtgga gagccggata aattttttca tagatttaac ccaactttgg atttgaatgt 78180 tattgacaga gatgttcatg cagaaatttt agataagttt aaagttgata ttggatttgg 78240 attagaaaaa catttgcagc gaacaaatgg gtctggaatg agtttatcta atcgcattat 78300 gaaagccctt aataaaattg gagcattgtc tcgtattaac gcgagtgaaa tccttcgtaa 78360 ttataataaa ggatatgacc tttatggtcg actaatgcca aaattatcat tcgatcaaat 78420 gattgcggat ttgtgggaaa atcaaagacg attattagca ttaggcgctc gattagctaa 78480 aggtctagat aaacaaatga tttttaagac taataataca gaagacctta aatgctttaa 78540 atttagtact cgtggcgatg attattacat cagagctcac tctacagatt atgttaatat 78600 ggggcatcat ctctgtttag cttttgaagt tttaaaagaa tctggaacat tagaatattc 78660 atctggtgca aaatgcccta ttggttcaag ttgtatttta atttatcgcc cggatgaatc 78720 cagttcaatt acattgccta caaaacctgt accagttcat agtaacgaaa aacattttga 78780 acaaattgat tattttaata aacagattga agagctgaat atttctattc aacaatatga 78840 tgatgaaatt ttcagactat ctggactgaa tagtaaagct aaatctgagc gtgaaaagtt 78900 aattaaaatt gttgatttac ttaaatctta aggaacacca tgaaaactcg ttctcaaatt 78960 gaagatatgg ttcgtactgc cagcgatact cgtaatgtta tgacattttt gtgtgaaaat 79020 aatttagacc ctgataaagt taatcgtgct attcatcact ttaaatatct aaatagcagt 79080 gaatgggtga gtaattttag taaagcgggg tatattacac aaatgactgc tcgtgagcag 79140 ctcacagatt tctgtaaaac tattgattat aaaaatcctc tatttgttca aggcgttggt 79200 caaagtaagg tcgatttatc atctggattt ttcaatccaa atcattatcg tattgaatgg 79260 agatttattg ctctattccg taaacaatta aagcaaattt tgtcgacggc tagtcgatta 79320 aaaggttctg acattaactt aaagaacctg aaatttgatg gttatactct tcagatggaa 79380 gtaagaccat taaaagaaaa taacagaact gcacgaatta gctttaaacc taatacaaaa 79440 aattctcttt caatttgcga atgccttaaa tcgcagttga cagaagcatt taagtatatg 79500 gatgttgttg ccgctgttca gtctaagatt ttacctcgtt ttgagcgatt taaattagac 79560 acaacaacat atgaacttga tatgatcgtt tcatttaaat acgatttttt gagaaaggac 79620 gaagtcccgc aagagaaaaa gcaggaagtg caagatatct taaatttatc caattactca 79680 tcaaacgatc ctaaattttg gatgtatagc tcaagcaata tagatgcatg taaacttaat 79740 aaagtgagtt ttcttcctac tgaaaattca aattttaaac ctgtagaaaa atggcacgcc 79800 gatgcgattg agaagtctct taaggcagta gatgatgaac tcgttaaagc gaccaatgaa 79860 gtgctagaag ctgaaaaggt gttagaacaa gctcagtcaa gggttcaaaa tctgacaaag 79920 caacgttcta aactgaacaa tgcactaaat gcactgaact agtttacttt gccacaagga 79980 tgtggtataa tgttcttact ttctactgag gagattaata tgactcgtaa cgaatatatc 80040 aaatcgttca atagcgttat tgatgataaa gttaaaccta tgtctagtca gaatagtgtg 80100 atttctatta tcaatcaatg gatcaataat actagtgcaa gtattgtttc ttccgacaaa 80160 tttatttatg aagtttgtga aatttctagt cgtacatata aagatgacat taaggaaacc 80220 tttaaaggtt ctcgtcttct ttcatactta gtcaacagag atattcttag taaatttggg 80280 aaagaaatta aacgaactaa agatgtagta ggatataatt ggttcggtga tgttaattct 80340 tatcatctta ataataaaga agaccctgag aatattttta ctcgtcgttg gattagtaat 80400 ttcagacttt tcaaaggaca aattctaaaa tcagcttcta aattatgtta tggcgattat 80460 cgtcaaattc atcctttggc ttctgatatg attatcgtaa aagaaaatga gcttgataaa 80520 aataaagtag ctatttttgt gaattatgga ttttttacac caaacgctaa ccaaaagaat 80580 attaataaat ttttctcaat tgctagcact ataacttatc aattagaggc ggctttgggc 80640 tgtatggaaa ctgtaaaaag tattcataca tatcctttca aaaatatgtg tggttgggaa 80700 ggatataaaa ttataattag ccttcgtgaa gtgaaatgtg cgtactcacc gactgataaa 80760 gaaatttacc aacaaaaatg tgaagaaatt gtgaatactc ctaaagaaga aactaccctt 80820 gaggaactaa tggataatct tgatgattca cctgaaccgg tagaaattcg tccagaagtt 80880 attgcattag aaaaagctta taaggaagtt ctagaaattt ctaataaagc gcagaaagaa 80940 tatgagcagg ctaaaaggat ttgggaagaa tctgttaatc gcctggatcg tcttgaacaa 81000 gctttacaat taattaagta atttaaagcc aaggatggct cggagtataa atcattaacc 81060 aagtgagaag aacatgaaaa ctcgtaaaca ttatattgat tattttgaca gtcttattac 81120 taaacaccgt aattatcaga tcggacacag agcagtaatc aataatattc ttcgtgattt 81180 ccttcaatat gttggccagg aaaatcatat ctgtaaagat actcaaaacg cttattctca 81240 ttcgttaggc aatttgcttg aatggtttaa acgttctcgt atgttatctt ctactgtagc 81300 tcgcgataac attaaaaact ttatgaagcc gagcttcatt aaatctacga catctataac 81360 cgatttggtt gaatttacta ttgttaacga tgttaaaaag acccatttgg ctgattggtt 81420 atctaccatt cctgaaacta aatttgctga taaatttgct tgtcaattca atgcccaagt 81480 gaatatgctt tttaagcatg ctcgcaaact atttaccgca ggcgatgacc gtacaaatac 81540 agttcatgtt aaggactggg ttattgctga tgaagtaaca cacaagccag gtggttcatc 81600 agtattaatt aatatccagg ttccttatta ttactcacgt aatctcggca ccatgacagc 81660 taaagaaatt aataagcaca acaaaactat tcgttcgttg tcttataaac ttcgcatgat 81720 gttaaaaatt atggacgtag tggaaatgta tgatgaaacc gaagataatg gttcaatgtt 81780 atacagctca cgcattctaa ttaaactgaa gaaccctaat acgtataagc cagttgtaaa 81840 agaacctaaa gtagaaaagg ttgataacct gagcgaagaa cgtgaatatc tcaatgctcg 81900 tttaattgaa gttgaagccc agattgccga acacaccaaa ttgttaaaag ctcttaatgc 81960 aaaagcaaat ggtttacgta atgctattga ggtattgaaa tgaaaaagcg cttattagaa 82020 gatattgcag cttcaagtaa ttccagtcta attaaaatta ttatggctgg tgaagaagac 82080 gatctggaaa tgcgtggaaa gattcatggt tgtgacgatt tagattttaa acctccagca 82140 tgggatgcta ttatggctat ggttgaacgt cgcgaaaggg cttctaaaaa cgttcctaat 82200 tgccctgaat gcggtactga acaggttcaa ttaattcatt ggcagactag tattcttcgt 82260 tataaatgcc gtcattgcaa acaccgattt aaccgagaag aaaatgacaa agcgtaaaga 82320 atatatggag actgctgaaa aggcagtccg tgaattagca atagcgtatt ataatgaaca 82380 tggtaaattt cctgatagat acagcgtgct taaatctgct ttaactcgtt catataaaaa 82440 tatgctatca gaagtaagtg atattatata caaacataaa gaacaaacgg gccaaagtct 82500 tgattacgac gagactttta aacaagtact aggaattaag gaataatatg tttaaagtat 82560 atggttatga tagcaacatc cacaaatgtg tgtattgcga taatgcaaaa cgtcttttga 82620 ctgtgaagaa acagccgttt gaatttatca atattatgcc ggaaaaaggt gtttttgatg 82680 atgagaaaat cgctgagctt ctgacgaaac taggtcgtga tactcaaatt ggtttgacaa 82740 tgccacaagt atttgctcct gatggaagtc atattggtgg atttgatcaa ttgcgggaat 82800 actttaaatg atgctcgaaa gaactgatta tatccatgac taccgcggaa gcgcggtata 82860 tgtaggtgat gaggttgcag tttattatgg atatggaact ttgatgacag caaaggttat 82920 tcaaattaaa aatggccgcg ctaaactcga agtttattat tctaatggtg aaaagtctat 82980 ttctaaatgg aaatatggcg attgtatggt caaactgggg taaatatgat ttacgatatt 83040 agtgtatcaa gagctccatc aatggttact attccagctg aagaactgga tcgtcttcag 83100 aaaattgaag aacttctttg ggaaattgaa tctgatttgc catcaggact agaatcctgg 83160 attgattatg aagaacttaa taaattgcga aattaattgg tgaaaaatga atattgaaaa 83220 taaattagat gttgatgcag ttctgagcga aatcatcgaa gaccatgatg gattttcaga 83280 aaattatgat ttcgattttt cggattatct taaacctgtc gaaatagaag attgggtaca 83340 agatggcaaa tgccaatatc gtcagtgtgt ttattttagt ccaaaacata acgttcatgt 83400 gtctgtaaat gaaactcgct caggttctta ccattctgac tggtattatg cagttccgac 83460 tgttgaacta gtcgagcccc atgaacgtgt agtaactcag acaatacgag aatggattac 83520 gctataaaac cttggtgggc ggctagatgg gaaactgtag agccagagcc ggaagaaccg 83580 gtttacactg atgaagaaac agtatataat gaaccaacga taaacgactt aattgatatg 83640 gagatgggat atgattgcag tagataagtg gtttaaaatt aatcgtgctg ataaagagct 83700 gtgtaattac tggccggaac ttggtgcagg tactgtcttt aaagttcgtg aacttgtaaa 83760 agaatgtgaa gatgatatag aacctgatac tggaattatt gaaattgaac tttctggtgg 83820 aaagattatt aacatctacg ataagccaac tgcctattgg tgcttatgga atactgaatc 83880 agtagaaaat ggcgaaattg aagaggtcgt agaacgagtt gatcaagtta ttcggaaacc 83940 tgaagccgct tttcaaggtg aacgtatttc atacgcatta gctaaattag ctgcacaaga 84000 aaataacgat ggttatgaag gaaatttaat gcaagctgcg gcagaatata ttgaatggct 84060 tgaaactcaa aattcttttt ctgaccgagc gttttaatga aaaccgaatt aatttatact 84120 gaaaagttaa atggtggtaa ggtttggaaa ctttttatta aaggaagtca taccgactct 84180 aatatgacca cttgtgtagg aacttattct cgtcctacta aaaagatgat tcggcaatat 84240 aagcgattgc atcgaatgtt ttacaatact tgaaaataat aaataccctt atctattcaa 84300 ggtaagggtt ttattatgtt attgactggc aaattataca aagaagaaaa acagaaattt 84360 tataacgcac aaaacggtaa atgcttaatt tgccaacgag aactaaatcc tgatgttcaa 84420 gctaatcacc ttgaccatga ccatgaatta aatggaccaa aagcaggaaa ggtacgtgga 84480 ttgctctgta atctctgcaa tgcagcggaa ggtcaaatga aacacaaatt taatcgttct 84540 ggcttaaagg ggcaaggcgt tgattatctt gaatggttag aaaatttact tacttattta 84600 aaatccgatt acacccaaaa taatattcac cctaacttcg ttggagataa atcaaaggaa 84660 ttttctcgtt taggaaaaga ggaaatgatg gctgagatgc ttcaaagagg atttgaatat 84720 aatgaatcta acactaaaac acaattaata gcttcattta agaagcagct tagaaagagt 84780 ttaaaatgac aattgaaaaa gaaattgaag gattgattca taaaactaat aaagaccttt 84840 taaacgagaa tgctaataaa gattctcgtg tttttccaac tcaacgggac cttatggctg 84900 gtattgtgtc taaacacatt gccaaaaata tggtcccgtc ttttattatg aaagcgcatg 84960 aaagcggaat tatccatttc catgatattg attattcccc tgctcttcca tttactaatt 85020 gctgtttagt agatttaaaa ggaatgcttg aaaacggatt taagctcggc aatgcacaga 85080 ttgaaactcc taaatcaatc ggcgttgcca ctgcaattat ggcacaaatt actgcacagg 85140 ttgcttccca tcaatatggc ggaacgactt ttgcgaatgt agataaagta ctttctcctt 85200 atgttaaacg cacctatgca aaacatattg aggacgcaga aaaatggcaa atcgctgatg 85260 cgttgaatta cgcccagtct aaaacagaaa aagacgtata cgatgcattc caagcttatg 85320 aatatgaagt aaatactctc tttagttcaa acggacaaac gccttttgta acaattacat 85380 ttggtacggg aactgactgg actgaacgaa tgattcagaa agcaattctg aaaaatcgta 85440 ttaaaggtct tggtcgcgat gggataactc ctattttccc taagcttgtt atgttcgttg 85500 aagaaggcgt taatctttat aaagacgatc cgaactatga tattaagcag cttgctctag 85560 agtgtgcaag caaaagaatg tatcctgaca tcatttcggc taagaacaat aaagctatca 85620 ccggttcatc tattcctgtt tctccaatgg gttgtcgtag tttcttgggc gtatggaaag 85680 attcgactgg taatgaaatt cttgatggac gtaataatct tggcgttgta acactgaatc 85740 ttcctcgtat tgcgttagat tcttatattg gaacacagtt caatgaacag aaatttgccg 85800 aattgttcaa tgaacgaatg gatttatgtt ttgaagcatt gatgtgtaga attagttcct 85860 taaaaggagt taaagctact gttgctccta ttctttatca agaaggtgca ttcggggttc 85920 gtcttaaacc tgatgacgat ataattgagt tatttaaaaa cggtagaagt tcagtgtccc 85980 taggatacat tggtattcac gaattgaata ttcttgtcgg tcgtgacatt ggacaagaaa 86040 ttttaactaa aatgaatgca catcttaagc agtggactga aagaactggt tttgctttta 86100 gcttgtattc tacacctgct gaaaacttgt gttatcgctt ctgtaaactc gataccgaaa 86160 aatatggaag tgtaaaggat gttactgata aaggctggta cactaacagt ttccacgttt 86220 cagtagaaga aaatattact ccgtttgaaa agatttctcg cgaagcacca tatcatttca 86280 ttgcgacagg tggtcacatt tcttatgttg aacttcctga tatgaaaaat aacctaaaag 86340 gtcttgaggc tgtatgggat tatgctgcac aacatttaga ttattttggt gttaacatgc 86400 cagtagataa atgttttacg tgtggaagta cccatgaaat gactcctact gaaaacggat 86460 ttgtttgttc tatttgtgga gaaactgatc ctaaaaagat gaacacaata agaagaacat 86520 gcggctattt gggaaatccg aatgaacgcg gatttaatct cggcaaaaat aaagaaatca 86580 tgcatagggt taaacaccaa tgaattatga tagattttat ccttgcgatt ttgtgaatga 86640 taaattatct ttaatatttg aagaaaataa tgatttacta ttgtcatatg ttgatttgat 86700 aacaaaaaat tataataatc ctaaaataaa aggaaaaacg gaagcacatc acgtgcttcc 86760 caaatctata tttcccgaat ataaaacaga taaatggaat attgttaatt tgtcatatta 86820 tgaccacgcg ttagctcatt actatttagc taaaacaagt aatagtaaaa tgtgttatgc 86880 ctttcataga atgataaact cgaataccga tagatttaat acagatgaat tggatgaaat 86940 tcttaaattg tattccagtg aaaaggaatt atttcaccgc tctatgtcaa aattatcgaa 87000 aaatatatgg aaggatgacg attttagaaa aagacataga gaactaacta agttagcaat 87060 gcaaaaacct gaagttagaa ataaaacttt attaaatttg tcaaatgcaa tgaaaactga 87120 cgaagttaga aataaaattt ccaggctaac taagttagca atgcaaaaac ctgaagtaaa 87180 agaaaaacat agaaattcat tgaggttggc gtatgcaaat cctgaggtta tgaaaagaaa 87240 acatcaacgt cttcgagaaa aatatactct taatttattt ggcgcatata aagatggtgt 87300 tcttttaaaa acatttaatt atataccaga cgcagcaaaa tattttaata tttctagcaa 87360 aggaaatatt ttaaaatgtt taaatggcga acgtaaaagt gttgaaggct ataattggaa 87420 atatataaat gaattatgac agaatatacc cgtgctgctt tgtgaatggc cctggatgca 87480 ggaccgttct tttcgttacc ggttgtttgc ataaatgcga agggtgttat aataaatcaa 87540 cgtggaacgc tagaaatggt gttccattca cgggtgaaac actagaacaa ttaattgaat 87600 gtttgaataa tgattatata gaaggattaa ctataactgg aggagaccct ctctatcctg 87660 ataaccgaga tgatgttcat tgcattgttc aaacagtaaa aaatctttat ccaaataaaa 87720 gcatttggtt gtggacagga tataagtttg aagatattaa acaactagaa atgcttaaat 87780 atgttgatgt tattattgat gggaagtatg agaaaaatct tccgactaaa aagctgtggc 87840 gaggatcaga taatcagcga ctttggtcaa ataccgatgg ggtgtggaaa catgattaaa 87900 ttgaattata ttatggacac tataaatgat atgatttttc attttggtcc agaattttat 87960 tcgcaatata gtttagtgct tatcaatgct tggttaatca attaagggta aaatatgtat 88020 aaatttcgca aaggtttagc tgattttctt acaactgtaa cattctttct gtttatggca 88080 gttggagcta ttttccttat tccttttatt gctatatttt tcgtgattag tttgatttct 88140 ccagaaaaag gactgtcttc tagcgagttc aatgagcgtc tggataaaat tactaataag 88200 ctgaatgctg ctcttagtaa ggaatagttg tgaaacagaa caagattgaa gtctatggaa 88260 ttccagatga agtaggtcgt tgtcctggat gtcaatcagt tacaaaactt ctaaaggagc 88320 tcaatgctcc ttttactttc tataaagttc ttacaaataa tggtaagatt gagtatgatc 88380 gtccactgat tgtatctctt gctaaacgcg ctggattcac atctcttaac attcgttatc 88440 cagtcatttt catcaatgat tctagacaaa agaacattaa acacttcaaa gaaactctca 88500 tttcacttgg atatgataga gatatcatag aagactaaga tgggccctct gggcctttct 88560 ttctcacatt ctgtatatta ccattctaac tatcgttccc ttcttatcat tccctaaaat 88620 aattttcaca aagttgttta caagaagttc aaactgtggt attattaaca catgaattgc 88680 ctttgaggaa ttgatatggt tgtggttgat aaagaaatta aaaagggaca gtattatttt 88740 attaatggta atgttgttcg tgttacttat gtaaacggtt ttgaagttta ttatcttata 88800 ctcaagttac ataaacagat gatttgtgac cgtgctgtat ttagttcagt tgctaaggaa 88860 attaaactcc atgggtaaaa catatcgtcg taaagattta aaagtacgtg attatgacta 88920 tttcggaaag cgtaaagctc ctgatggtgt aagtcataaa gatatggttg aaaacatttt 88980 tcgctctgat aaatggcgta gaatgaaagg cattgattca gaagttaaag atgagttaaa 89040 tcgtcaattg cgcggtgaag taagaaagtt gaaaaaatca gtttacattg acgatgattt 89100 tgattacaat acttcccaac gagttgctaa gcgcaaatca aacgagtgtt atcgttacag 89160 ctgaggaaaa tatgaatatc aaacgcatgc tttttaaaca aggattatac actttaaatg 89220 ttgctccaaa aggcgatacg accaagtggt cagtaaatga ctggattaaa tttattgatg 89280 aaaatggtaa ctggaaaatt taaatgaatc ctgaatctaa attatcgcaa cgaattgctg 89340 aagctcgtcg tcagttcttt gaaaatatga tagcccaagg tattgatgac gaaatttttc 89400 taaactggtt ttggaatcac aaatattcaa aatgcgcagg agcaatatta atatcagccg 89460 caatgatgta tgaaggctgg aagggtgcca aacagtttag ctaagggctt cggccctttt 89520 tggataataa aattttaatg taattgagga taatgtatga ctattcaaat taaaaacgcc 89580 atcaattctt acgcatatga taaagtagtt tctttgctag aaaaaggcga tattgtaact 89640 cctcaaattt tggataaatg ggaaaaagag cttcatcaga cgatgaaaca gaatgatcag 89700 aaaattggtc gcaatactgt ccgtgaattg ttggttcaat atatcttgtc agaatttgat 89760 gttaaagctt ttggcgtaga atctaaagct tatcaaaagc atgaaatttc cgataaaact 89820 attcgtcgta tgaaaaatca acgcaagaaa aaatttgctg acctgaaaat tgctaaggta 89880 tgaatatgaa cgaagctctt attaacgatt tgcgtcttgc cggatatgaa gtaaatacaa 89940 acggcattgg tttaactcaa attgaaggaa atggattcat ccttgagtat gaatttagcc 90000 aatggtggtt atacgctaat tatggcgaat tgattgaata cgttgaccag tttgattcac 90060 tagatgcagc tcttgaagcg gctaagttga tgaatgcatg aaatttatta atatttctat 90120 tactattgaa aattatggca ttttctatgt tgatcaatac atgaaaattt catttttccc 90180 aaataagact ggtgttggat attgggaaag ccatgtttct gaattaaatg aaagtgaata 90240 tgctagcaca catgaaaagt ttttagactt tttatatcat gctgatttaa ctagtcatta 90300 tatagatatt catgaattta aaaagatgat ggagaaagtg ttccaggcat actgcttact 90360 tagataactg atatcctcta tgctttaaga tagatcttca aatattatga tataatagat 90420 ctatgaattg agctaagagg tgaaaatgtc agaaactaag cctaaatata attacgtaaa 90480 caataaagag cttttacaag ctattattga ttggaaaaca gaattagcaa ataataaaga 90540 cccaaataaa gtagttcgtc agaatgatac tatcggattg gccattatgc ttattgcaga 90600 aggcttatct aaacgtttca acttttcagg atacacccaa tcctggaaac aagaaatgat 90660 tgcagatggt atagaagctt ctattaaggg gcttcataat tttgatgaaa cgaaatataa 90720 aaacccacat gcatatataa ctcaagcttg ttttaatgca ttcgtccaac gtattaaaaa 90780 agaacgtaag gaagttgcaa agaaatacag ttacttcgtt cacaatgtct atgacagtcg 90840 tgacgacgat atggttgcgt tagtagatga aacttttatt caagacatct acgataaaat 90900 gacgcattac gaagaatcaa cctatagaac accgggggct gaaaagaaaa gtgttgtaga 90960 cgattctcct agtttggatt ttttatatga ggctaacgat taacctctcc ggattcttgg 91020 aagaaatacc tgaagttgaa gctattccct atttacttaa aatgtatctc agggaagttt 91080 tagctcttga cattgatatt gatccagaaa atccgtatga taccgctttt aaatctaatg 91140 gtgtagaatt aaactatcgg tatcatttaa cagatgatga tttttatttt atattagaga 91200 aataatatga ctgataaacc cgaaattaat gatgaagtgg aaaagcttat ttcttctatt 91260 gaagaaaaga accgtcttga agcagaaaga aaagcaaata agttattgtc taaaaacaaa 91320 cgcgaactga accgtcttta taagcacgct cagattgcag ctgaaaacaa taattttgct 91380 caatacgaat atgctatcaa gaaaagtcgg gatattctaa aacagccata taacgatgaa 91440 ctcattagta ttctttggaa gactactaga tcgcagattg aggatatgat tgatgcttac 91500 acacgtaaaa ttcaagcgtc ttaaaattaa tgcaggattt actgaatctt tgaatggtca 91560 tctttgtgtg aaaatttctg aaaaagaata ccacgatagt tcaattaaag aagttaatcc 91620 tcctattgta agagcagacc ctaatatgaa agtgtgggtt gattcttatc aagtcaaaaa 91680 atggtggcaa ctgtgaaaga tgaacaccca gacttctgaa atagattata ataaaattcg 91740 ttcctctaaa gtagaaatga tgagacgctt taaagagtct catgataaag ctaaagcaga 91800 aggaactata acatataagc gtataaaatt tagaagctct aatgagcctc tgtatggtgt 91860 attatgcgga taggagcttc ggctcctata ttgctttata aatttttggt aaaataaacc 91920 aaaacaatga ggatattaaa tgaaagtatg catttttatg gctcgaggtc ttgaaggttg 91980 cggcgtaact aaattttctc tcgagcaacg tgattggttt attaaaaatg gtcatgaagt 92040 aactttggtt tatgctaaag ataaatcatt tacccgtaat tgcgcgcatg attataaatc 92100 attttcaatt ccggttttat tggcaaaaga atacgataaa acacttaagc tggtaaatga 92160 ttgtgatatt ctaattatta attcagttcc tgctacttca gtagaagaag acactattaa 92220 taactataaa aaaattattg ataacattaa accttcagtt cgtgttgtag tttatcaaca 92280 tgaccattct tctctttctt tgcgccgaaa tttgggatta gaagaaactg ttcgtcgagc 92340 tgatgttatt tttagtcatt ctgataatgg cgattttaat aaagttttga tgaaagaatg 92400 gtatccagaa acggtttctc tgtttgatga tattgaagaa gcaccgaccg tatataactt 92460 tcagcctcct atggatattg cgaaggttcg atcaacctac tggaaagatg tttctgaaat 92520 taacatgaat atcaaccgtt ggattggtcg tactactaca tggaaaggct tttatcagat 92580 gtttgatttt cacgaaaaac atcttaaacc tgctgggcta agtactatta tggaaggtct 92640 ggaacgttct ccagcgttca ttcctattaa agaaaaagga attccatatg agtattatcg 92700 tcttcatcaa gtagaccaaa ttaaaattgc tcctaatttg ccaacgcaaa ttcttgaccg 92760 ttatgtaaat agcgagatgc ttgaacgcat gagtaaatcc ggatttggtt atcagttgag 92820 taagttggac aaaaaatatc tacaacgttc tttagaatat actcatctcg agcttggtgc 92880 atgtggaaca attccagtat tttggaaatc tactggtgaa aatttaaaat tccgcgttga 92940 taatactccg ctaatttcac atgatagtgg tatcgtttgg tttgatgaaa atgacatgga 93000 atcaacattt gagcgtatta aagaactgtc atctgaccga actctttatg accgtgaacg 93060 cgaaaaagct tatgaatttt tgtatcagca tcaagattca agcttctgct ttaaagaaca 93120 atttgacatt attacaaaat aaagggcttc ggccctttag ctttatacgg agtttgatat 93180 aatgatattt cttggatatg tgatactttt tctcgcattt tatctattca ctagagcatg 93240 ttggattggg ttctttagca ctccagatgg atttatttca ataattttat tttgcatttc 93300 aatgacggtt cttgatatat gaaaatttta aatttaggtg attggcattt aggtgttaaa 93360 gccgatgatg agtgggttca atccattcag ttggatggaa ttaaacaagc aatagaatat 93420 tctaagaaaa atggaattac tacatggatt caatacggcg atatttttga tgtgcgaaaa 93480 gcaatcacac ataaaactat ggagttcgct cgtgaaatag ttcaaatgct tgatgacgct 93540 ggaatcacat tacatactat tgtaggaaat cacgatctcc actacaaaaa tgtaatgcat 93600 ccaaatgcct ctactgagct tttggctaaa tatcctaatg ttaaagtata tgataagcct 93660 gctacagtag attttgacgg atgtttgatt gatttaattc cttggatgtg cgaagaaaat 93720 actggtgaaa ttcttgaaca catcaaaact tcatctgctt ctttttgtgt tggtcactgg 93780 gaactgaatg gattttattt ttataaagga atgaaatctc acggtcttga acctgatttc 93840 cttaagactt ataaagaggt gtggtctggt cacttccata ctatctctga ggctgctaac 93900 gtcagatata ttgggacacc atggacacta actgcaggtg acgagaatga ccctcgcggg 93960 ttctggatgt ttgatacaga aacagaacga atggaattta ttccaaacaa caccacctgg 94020 catcgtcgaa ttcaatatcc atttaagggg aaaattgact ataaagattt tacaaatcta 94080 tcagtacgtg ttatagtaac tgaagtagac aaaaatctaa cgaagttcga atccgaacta 94140 gaaaaagttg tgcattcatt acgagttgtg tcaaagattg ataactctgt cgagtcagat 94200 gatagtgaag aagttgaagt tcaatctctt cagacgttga tggaagaata cattaatgca 94260 attccagaca tcactgattc tgatcgcgaa gcacttattc aatatgcaaa ccagttatat 94320 gtagaggcaa cacaatgact tttgatgaat ttaaaaatgt tatgatgagt cagcattttg 94380 aatgcgaagt aaaagatgat attgggcata aagaaattat tgaatattgg tttgaaccgc 94440 tagaggttga agataattgt attaaaaagg ttacggtctg cactgattgg gccgtatctt 94500 ttaacttcaa cattttagat aatgacacac ctaaatcatt acaagatatg gctgtatctt 94560 gtattaagga tgcatactgt gaagttttcg acatttgaca ttaacgatga attcatagca 94620 aatattgatt acaccgaaga agattctaga tatgttggaa taatttatat cacatcaaaa 94680 gcagcacaag gtgttgtttg catggctgaa tttgatgaat actttttaga ttatgatgat 94740 atgatagaat ggtctaaaag atacattaaa aggaatcttt tgtgaagaat tttaaactaa 94800 accgagttag gtatcaaaat ataatgtcag taggtggaaa tcctattgac attcaactag 94860 ataaggttca aaaaactctt attactggac gaaatggcgg tggtaagtct actatgttag 94920 aagccatcac atttgggctt tttggcaagc catttcgtga tgtaaagaaa ggtcaattaa 94980 taaacagcac aaataagaaa gaacttttag ttgaactgtg gatggaatat gatgagaaaa 95040 agtactatat caaaagagga caaaaaccaa acgttttcga aatcaccgtt aacggtacac 95100 gtcttaatga atctgccagc agtaaagatt tccaagcaga atttgaacag cttatcggaa 95160 tgtcatatgc cagtttcaag cagattgttg tccttggtac agcagggtat acccctttca 95220 tgggtttgtc aacccctgca cgaagaaagc ttgtggaaga cctgctcgag gtaggaacat 95280 tagctgaaat ggataagctt aataaagcac taatacgtga attaaattca caaaaccaag 95340 tgcttgatgt taaaaaagat agtattatcc aacaaattaa aatatataat gataacgttg 95400 aacgccagaa aaaattaact ggtgataacc ttactcgtct acagaatatg tatgatgatt 95460 tggcaaaaga agctagaacg ctaaaatcgg aaatagaaga agctaatgaa agattagtta 95520 atattgtttt ggatgaagac ccgactgatg catttaataa aatcggccaa gaagcatttt 95580 taattaaatc aaaaattgac tcgtataata aagtcattaa tatgtatcac gaaggtggat 95640 tatgtccaac ctgtttgtca caattaagtt ccggtgataa agttgtttct aaaattaaag 95700 ataaagtttc tgaatgcacg cattcgtttg aacagctttc aacacatcgt gataatttaa 95760 aagttctcgt cgatgaatac cgagataata ttaaaaccca acagacgttg gcaaatgata 95820 ttcgcaataa aaagcaatct ctaatcacga ctgtagataa agctaaaaaa gttaaagcag 95880 ctatagaaaa agcatcatcc gagtttattg accatgctga tgaaatagca ctgcttcaag 95940 aagaacttga taaaattgtt aagacaaaaa ctaatttagt aatggaaaaa taccacagag 96000 gaattttgac tgatatgctc aaagattctg gtattaaagg tgctattatt aaaaagtaca 96060 ttccattatt taataagcag attaaccatt atcttaaaat aatggaagca gattatgtgt 96120 ttacattaga tgaagaattt aatgagacaa ttaaatcccg tggtcgtgaa gattttagtt 96180 atgcttcatt tagtgaaggt gaaaaagcgc gaattgatat tgctctttta tttacttggc 96240 gtgatattgc ttctatcgta tctggtgtta gtattagtac attaattctt gatgaagtgt 96300 ttgatgggtc atttgatgcc gaaggtatta aaggtgtagc taatattata aattcaatga 96360 aaaatactaa tgtttttata atttcgcata aagaccacga cccgcaagaa tatggtcagc 96420 atcttcaaat gaagaaagtt ggtcgattta ctgtaatggt ttaatttata agagattatg 96480 ctttaattta ttagagtata atctctatgg aggaaaaaca tggaatattc aactggacag 96540 catctattaa ctattcctga aataaaacga tatattctga gaaataattt ttctaatgaa 96600 gagcatatag ttactgaatc tatgcttaag aatgcattta aatcagaata tacgaaaata 96660 atgtccaaca ggaatgaagc ttggactgtt actgattatt atgactaaag gtgtattatg 96720 actaaaatta ctgtgaatta tactgttgat gtaaaagata ttcagccaaa acacgtgcgt 96780 tctgaatcaa atccgcaaaa ccaaaataaa attcgtcgag catgggtttt gtctctttct 96840 gataacgcaa tggaagttat tcagaataaa attaaatctg cacctgctcg tcatgcgtat 96900 tatgaagcta tcgatcgtga agtgagtgat aaatggattg aactaatgcg caaacatacg 96960 acagaatctc taaatgctgg tgctaaattt attatgactt catgcggtga acgccttgaa 97020 gatgattatt gcggtaatgc agatgaacgt ctaattgttg ctgctcaaat tgttgcagaa 97080 acaattgctg ctgattttaa tcgttaattg ctttattaaa ttagttataa aattaaatct 97140 catttgaatt gaaggaaatt acatgaaact gtctaaagat actactgctc tgcttaaaaa 97200 tttcgctact attaactctg gtattatgct taaatctggt caatttatta tgactcgcgc 97260 agttaatggt acaacttatg cggaagcaaa tatttccgac accattgatt ttgatgtagc 97320 aatttacgat ttgaacggtt ttctcggtat tctgtcccta gttaatgacg atgcagaaat 97380 ttcccagtca gaagatggaa atattaaaat tgctgatgcc cgttcaacaa ttttttggcc 97440 tgcagccgat ccgagtacag tagttgctcc taataaacca attccattcc cggtagcatc 97500 tgttgttact gaaattaaag ctgaagacct tcaacagctg ttgcgtgtat ctcgtggtct 97560 gcagattgat acaattgcta tcacagtaaa agaaggtaaa atcgtaatta acggttttaa 97620 taaagtagaa gattctgctt tgactcgtgt taaatattct ttgactcttg gtgattatga 97680 tggtgaaaat acatttaatt tcattatcaa tatggcaaat atgaaaatgc aaccaggaaa 97740 ttataaactt ctgctctggg caaaaggtaa acaaggtgct gctaaatttg aaggtgaaca 97800 cgcgaattat gtagtagctc ttgaagctga ttctacccac gatttttaat agagggcttc 97860 ggccctttat aatttacact aaaacttgaa tgaggaaatt atgattactg taaatgaaaa 97920 agaacacatt cttgaacaga aatatcgtcc atctactatc gatgaatgta ttcttcccgc 97980 ctttgataaa gaaaccttta aatctattac aagtaaaggt aagattccgc atattattct 98040 tcattctcct tctccaggaa caggtaaaac aactgtagca aaagcattgt gtcatgacgt 98100 aaatgctgat atgatgtttg tgaatggatc agattgtaaa attgatttcg ttcgcggtcc 98160 tttgaccaat tttgctagtg cagcttcatt tgatggccgt caaaaagtaa tcgtcattga 98220 tgaatttgac cgttcaggat tagcagagtc tcagcgacat cttcgttcct ttatggaagc 98280 ttatagttca aactgtagca ttattattac tgctaacaat attgatggta ttattaaacc 98340 acttcagtca cgctgccgag ttattacatt cggtcaacca accgatgaag ataaaattga 98400 aatgatgaag cagatgattc gtcgattgac tgaaatctgt aagcgtgaag gaattgctat 98460 agctgatatg aaagttgtag cagctttggt taaaaagaat tttcctgatt ttcgtaaaac 98520 tattggagag ctcgatagtt attcatctaa aggtgttttg gatgctggta ttttatcact 98580 ggttactaac gatcgtggtg ctattgatga tgttcttgag tctctcaaaa ataaagatgt 98640 taaacagctc agagctttag caccaaaata tgcagctgat tattcgtggt tcgtaggtaa 98700 acttgccgaa gaaatctata cacgcgtaac tccacaaagt attattcgta tgtatgaaat 98760 tgttggtgaa aataaccagt atcacggaat tgcggctaat actgaattac atttagcata 98820 tcttttcatt caattagcat gcgaaatgca gtggaagtga tatgagctta tttgaagatg 98880 atattcaatt aaacgagcat caagttgctt ggtattcaaa agattggaca gctgtccaat 98940 ctgctgctga ttcttttaag gaaaaagctg aaaatgaatt ttttgaaata attggagcta 99000 ttaataataa aactaaatgc tctattgctc aaaaagatta ttcaaaatac atggttgaga 99060 atgcattatc acaatttcct gaatgcatgc cagctgtata tgctatgaat ttaattggat 99120 caggtttaag cgatgaagcc cattttaatt atctgatggc tgctgttcct cgtggtaaaa 99180 gatatggtaa atgggcaaaa ctggttgaag attctactga agtattgatt attaagttac 99240 ttgctaaacg gtatcaagtt aatacaaatg atgcaattaa ctataaatca attcttgcta 99300 aaaatggaaa acttccttta gtattaaaag aactaaaagg tttagtcacg gatgattttt 99360 taaaagaagt gactaagaat gtaaaagaac agaaacaact caaaaaacta gcattggaat 99420 ggtaaaatga ttgaaattac tcttaaaaaa cctgaagatt ttctgaaagt aaaagaaact 99480 ttgactcgta tgggaattgc taataataaa gataaagttc tgtatcagtc ctgccacatt 99540 cttcagaaaa aaggactata ctatatcgtc cattttaaag aaatgcttcg tatggatggc 99600 cgtcaggttg aaatgacaga agaagatgaa gttcgccgtg attcgattgc gtggctgtta 99660 gaagattggg gactgattga aatcgttcct ggtcaaagaa cttttatgaa agatttaacc 99720 aataacttcc gagttatttc ttttaaacaa aaacatgaat ggaaactcgt tccaaaatat 99780 acgattggta attaagcaag gggcttcggc cccttatttg gagtataata tatcaagagc 99840 ctaataactc gggctataaa ctaaggaata tctatgaaag aattttatat ctctatcgaa 99900 acagtcggaa ataatattgt tgagcgttat attgatgaaa atggaaagga acgtactcgt 99960 gaagtagaat atcttccgac tatgtttagg cattgtaagg aagagtcaaa gtacaaagac 100020 atttatggta aaaattgcgc tcctcaaaaa tttccatcaa tgaaagatgc acgagactgg 100080 atgaaacgaa tggaagacat tggtctcgaa gctctcggta tgaacgattt taaactcgct 100140 tatatcagtg atacatatgg ttcagaaatt gtttatgacc gaaaatttgt tcgtgtagct 100200 aactgtgaca ttgaggttac tggtgataaa tttcctgacc caatgaaagc agaatatgaa 100260 attgatgcta tcactcatta tgattcaatt gacgatcgtt tttacgtctt tgatttgttg 100320 aattcaatgt acggttcagt atcaaaatgg gatgcaaagt tagctgctaa gcttgactgt 100380 gaaggtggtg atgaagttcc tcaagaaatt cttgaccgag taatttatat gccatttgat 100440 aatgagcgtg atatgctcat ggaatatatt aatctctggg aacagaaacg acctgctatt 100500 tttaccggtt ggaatattga gggatttgac gttccgtata tcatgaatcg tgttaaaatg 100560 gttctcggtg aacgtagtat gaaacgcttc tctccaatcg gtcgagtaaa atctaaacta 100620 attcaaaata tgtacggtag caaagaaatt tattctattg atggcgtatc tattcttgat 100680 tatttagatt tgtataagaa attcgcattt actaatttgc cgtcattttc tttggaatca 100740 gttgctcaac atgaaaccaa aaaaggtaaa ttaccatatg atggtcctat taataaactt 100800 cgtgagacta atcatcaacg atacattagt tataacatca ttgacgtaga atcagttcaa 100860 gcaattgata aaattcgtgg gtttatcgat ctagttttaa gtatgtccta ttacgctaaa 100920 atgccttttt ctggtgtaat gagtcctatt aaaacttggg atgctattat ttttaactca 100980 ctgaaaggtg aacataaggt tattcctcaa caaggttcgc acgttaaaca gagttttcca 101040 ggtgcatttg tatttgaacc taaaccaatt gcccgtcgat acattatgag ttttgacttg 101100 acgtctctgt atcctagcat tattcgtcag gttaacatta gtcctgagac tattcgtgga 101160 cagtttaaag ttcatccaat tcatgaatat atcgcaggaa cagctcctaa gccaagtgat 101220 gaatattctt gttctccgaa tggatggatg tatgataagc atcaagaagg tatcattcca 101280 aaggaaatcg ctaaagtatt tttccagcgt aaagattgga aaaagaaaat gttcgccgaa 101340 gaaatgaatg ccgaagctat taaaaagatt attatgaaag gtgcagggtc ttgttcaact 101400 aaaccagaag ttgaacgata tgttaagttt aatgatgatt tcttaaacga attatcgaat 101460 tacaccgaat ctgttcttaa tagtctgatc gaagagtgcg aaaaagcagc tacacttgct 101520 aatacaaatc agctgaaccg taaaattctt attaacagtc tttatggtgc tcttggtaac 101580 attcatttcc gttactatga tttgcgaaat gctactgcta tcacaatttt tggtcaagtt 101640 ggtattcagt ggattgctcg taaaattaat gagtatctaa ataaagtatg tggaactgat 101700 ggtgaagatt tcatcgcagc aggtgatact gattcggtat atgtctgtgt agataaagtt 101760 attgaaaaag taggtcttga ccgctttaaa gaacaaaatg atttagttga attcatgaat 101820 cagttcggta agaaaaagat ggaacctatg attgatgttg catatcgtga gttatgtgat 101880 tatatgaata accgcgagca tctgatgcat atggaccgtg aagctatttc ttgtcctccg 101940 cttggttcaa agggcgttgg tggattttgg aaagcgaaaa aacgttatgc tctgaacgtt 102000 tatgatatgg aagataagcg atttgctgaa ccacatctaa aaatcatggg tatggaaact 102060 cagcagagtt caacaccaaa agcagtgcaa gaagctctcg aagaaagtat tcgtcgtatt 102120 cttcaggaag gtgaagagtc tgtccaagaa tactataaga actttgagaa agaatatcgt 102180 caacttgact ataaagttat tgctgaagta aaaaccgcga atgatatagc gaaatatgat 102240 gataaaggtt ggccaggatt taaatgtccg ttccacattc gtggggtgct aacttatcgt 102300 cgagctgtta gtggtttagg tgtagctcca attttggatg gaaataaggt aatggttctt 102360 ccattacgtg aaggaaatcc atttggtgac aagtgcattg cttggccatc aggcacagaa 102420 cttccaaaag aaattcgttc tgatgtactg tcttggattg actactcaac tttgttccaa 102480 aaatcgtttg ttaaaccgct tgcgggtatg tgtgaatcgg caggtatgga ctatgaggaa 102540 aaagcttcgt tagacttcct gtttggctga tagaataaat ctagggacct ccgggtccct 102600 ttttcataca agtaatataa atctatactt aggaaaaata gatgattctg gacctctgga 102660 attcattaaa aaatttttca caaaatggtt tacaagactg ttcctctgtg gtactataca 102720 actatcaacc aatacagatt tggagaatga aatgaaaatc gctattttag ttattgcatt 102780 gggtcttact ggttgtgtag ctcaaggacc agtagtaaat cagtctgatg caggaaaaat 102840 tgtaaactgt tcaagcaaat tttataatcc taacgtcaag tgttataaag aagctccaaa 102900 acagacagta gaagaaatgc aggcgaattt tgacgaagct attcgtccag atgaatctgc 102960 tcaagcatat cgtaattcag atgtgattac gcgtgaagaa aaaattgaaa actactgcgc 103020 tgaactttgg gctaattggg ctaataatta ccaatggcgt actggtaaaa atgctccgat 103080 ggagtatgta gtaaattctt acaattcatg cgtaaaaaat ttgactaagt gaggaaacat 103140 ggaaacttta gtagcaggtt caatttttat ggttttagtt tcaggcgtgt tggctattat 103200 tatatacatg cttccgtggt tcatcgcctt gatgcgtggg tcaaaatcga cagtaggaat 103260 cttttttaca tctttactgt ttaactggtc aatcattggt tggtttatta catttatttg 103320 gtcaattgcg ggtgaaacta aaaagtcaac acagccaaat caagtaatta tcatcagaga 103380 gaaggaatga aaagcaaaat tataacagtg ttgcttttaa tcttgatgat tataataagt 103440 atatactata gtgtaacggt tcctcttatg attccaacgc ttattatagg gtggagttta 103500 ttactgttac aagttaaata tgaatgtatc aattgaggtt taaatgatta atgactctat 103560 gacagttgaa gaaattcgtc ttcatttggg aattgcatta aaggaaaaag attttgtaat 103620 tgacaaaacc ggggttaaaa ctattgaaat tattggtgca tcatttgtag cagatgaacc 103680 ttttattttt ggtgctctta atgatgaata cattcagcgt gaacttgaat ggtataaatc 103740 taaaagtttg tttgttaaag atattccggg tgaaacgccg aagatttggc aacaggtagc 103800 atcttctaaa ggcgaaatta actcgaatta tggttgggcc atctggtcag aagataacta 103860 tgcccagtat gatatgtgtt tagctgaact tggtcaaaat cctgattctc gacgcggtat 103920 catgatttat actcgtccat ccatgcagtt tgactacaat aaagatggta tgtcagattt 103980 catgtgtacg aatacagtac agtacctgat tcgtgataag aaagtcaatg cagtagtaag 104040 catgcgttca aatgacgtag tcttcggatt ccgtaatgat tatgcatggc aaaaatacgt 104100 actagataaa ttagtatctg atttgaatgc aggtgattca actcgtcagt ataaagcagg 104160 ttccattata tggaatgtcg gaagccttca tgtgtattcc cgtcattttt atttagttga 104220 ccattggtgg aaaactggcg aaactcatat tgcgaaaaaa gattataacg gtgagtggaa 104280 atgattcaat tcgtaattcc aagctatcaa cgcgtagggg cagtttctgc ccttgatatg 104340 tttccatctg attatgaacc gcatattgta gtacgtgaac atgaagaaaa agcttattat 104400 gacgcttacg ggtctagagc caaaattata actattcctg atgatgttaa tggaattgct 104460 ggcactcgta aagcaattac tgatatgtat gcaggtcaac gaatttggat gattgatgat 104520 gatactacta ttcgtatgag ttcaatgcga aaaagagatg accgtcgttg tgtagataaa 104580 gtcaatcaat taacccgtga acagttctat gaattgattc aatacgtcga agatgccatg 104640 gattgcgggt attatcatgg ccatgctcgt ctacctattt ttaaaattac ttcatcttgg 104700 ggtaattatc gtgaaaattc atatggattc acgaatacat ggtatgacct cggaaaactt 104760 acaacagaac aaattgggta tggaaaaatt gatttatgcg aagatatgta tgcatttctc 104820 aatttaatta atcaaggtta tccgcatttg gccctgttca aatatctggt tgtatctgga 104880 aaagcgcaag ctccgggcgg gtgcagttca attcgtagta attcaaaaca taatagagct 104940 cttgagcaaa tcaatagaga gtttccagag caagctcgtt ggaaaacatc gaatattgaa 105000 aagagaaaat cgttgggtga agaagacgag ccattaaagg ttcttcgcat gtgtgtttcg 105060 cgcaaagaaa aatcagaagc gtttcataag tttaatgcta ttcatccaat agcagttgat 105120 taatgcctaa atttattgtg ttataattac tttatcttta accagtgagg aaaatataat 105180 gatgcctatg gaaaaaatga atgtctattg cagatttaaa atcccgtttg attaaagctt 105240 ccacttctaa aatgactgct gaactgacta catctaaatt ctttaatgaa aaggatgtaa 105300 tccgtacaaa aatcccaatg cttaatattg ctatttctgg tgcgattgat ggcggtatgc 105360 agtctggttt aactattttc gcagggcctt ctaaacactt taaatcaaat atgtctttga 105420 ctatggttgc agcgtatttg aacaaatatc ctgatgcagt ttgtctattc tatgatagcg 105480 aatttggtat tactccagct tatttgcgat ccatgggagt tgacccggaa cgagtaattc 105540 atacaccaat ccagtcagtt gaacagctga aaattgatat ggtgaatcag cttgaagcta 105600 ttgagcgtgg tgaaaaggtt attgtattca tcgactcaat tggtaatatg gcttctaaga 105660 aagaaacgga agatgccttg aatgaaaaat ctgtggcaga tatgactcgt gctaaatcac 105720 tgaagtcatt attccgtatt gttactcctt attttagcat taaaaatatt ccatgtgttg 105780 cggttaacca tacaattgaa accattgaaa tgtttagtaa aaccgtgatg acaggtggta 105840 caggcgtaat gtattcagct gatactgtat tcattatcgg taagcgtcag attaaagatg 105900 gttctgatct tcaggggtat caatttgttc taaatgtaga aaaatctcgt accgttaaag 105960 aaaaaagtaa attctttatt gatgttaaat ttgacggtgg tatcgatcct tattctggat 106020 tgttagatat ggctcttgaa ttaggatttg tagtaaaacc taaaaatggc tggtatgctc 106080 gtgaatttct tgatgaagaa accggcgaga tgattcgcga agaaaaatct tggcgtgcaa 106140 aagataccaa ttgcactaca ttctggggtc ctttatttaa gcatcaacca ttccgagatg 106200 ctattaaacg tgcttatcag ttaggtgcta ttgatagtaa tgaaattgtt gaagctgaag 106260 ttgatgaatt gattaactca aaggttgaaa aatttaaatc tccagaaagt aaaagtaaat 106320 cagcagctga tttagaaact gacctagatc agttaagtga tatggaagaa tttaatgaat 106380 aaagatgatt tagatttaga tctagaaatt atcgacgaat ccccctcttc ggagggggaa 106440 gaagaaagaa aagaacgcct ttttaatgaa tctcttaaga taattaaatc tgccatggaa 106500 aatgttatcc aggagattgt cattaaacta gaagacggtt ctacacacat tgtgtatgtg 106560 acaaaattag attgggttga tggaaaagtc gtaatggact ttgctgttct tgaccaagaa 106620 agaaaagctg agttagctcc tcatgtagaa aaatgtatta caatgcaact acaagatgca 106680 tttaataaaa ggtcaaagaa aaaatttaaa ttcttttaag gagtaagtgt ggtagaaatt 106740 attctttctc atctcatatt tgatcaagct tatttttcaa aagtttggcc atatatggat 106800 tcagaatatt ttgaaagtgg tccagctaaa aatacattca aattaattaa atctcatgtt 106860 aacgagtacc atagcgttcc atctattaat gcgttaaatg ttgcgctaga aaatagttca 106920 tttactgaaa cagaatattc tggtgtaaaa acacttattt caaaactggc cgattctccg 106980 gaagaccata gctggttagt aaaagaaaca gaaaaatatg ttcagcaaag ggcgatgttt 107040 aacgccacat ctaaaataat tgaaattcaa accaatgctg agcttcctcc agaaaaacga 107100 aataagaaaa tgccagatgt aggtgctatt cctgacatca tgcgccaagc attgtcaatt 107160 tcatttgata gctacgtcgg tcatgattgg atggatgact acgaagcacg ttggctatct 107220 tatatgaata aagctcgtaa ggttccattt aaactcaaaa ttctaaataa aattactaaa 107280 ggcggagctg aaactggaac actgaacgtt ttaatggctg gtgttaacgt tggtaagtca 107340 ttaggattgt gttcattggc agcagattat ttgcagctcg gacacaatgt tctttacatt 107400 tccatggaaa tggcagaaga agtctgtgct aagcgtattg acgctaatat gctcgatgtt 107460 tctcttgatg acattgatga cgggcatatt tcttacgctg agtataaagg aaaaatggaa 107520 aaatggcgtg agaaatctac tcttggccgt ttaattgtta aacagtatcc tactggtgga 107580 gcagacgcta atacatttcg atcgctttta aacgaattaa aactcaagaa gaattttgtt 107640 ccaacaatca ttattgtcga ctatctaggt atttgtaaat cttgccgcat cagggtttac 107700 tcagaaaata gttacacaac tgttaaagct attgcagagg aattgcgtgc tcttgctgtt 107760 gaaaccgaaa ctgttctttg gactgcagcg caggttggta agcaagcttg ggactcttcc 107820 gatgttaaca tgagcgatat tgcagaatct gccggtcttc cagcaacagc cgattttatg 107880 cttgcggtca ttgaaaccga ggagttagca gctgctgaac aacaactcat taagcaaatc 107940 aaatcacgat acggtgataa gaataaatgg aataagtttt tgatgggggt tcaaaaagga 108000 aatcagaaat gggtagaaat tgaacaagat tctactccaa ctgaagtgaa cgaagtagca 108060 ggttcacagc agattcaggc tgaacagaac cgctatcaaa gaaatgaatc ggctcgagct 108120 cagttagatg ctttggcaaa cgaattaaaa ttttagttta caagccgaca agactatggt 108180 atagtagtct tgttggttaa atgaggagat tgttatggaa ttggtaaagg tagtttttat 108240 ggggtggttt aagaatgaaa gcatgtttac taaagaaatc gcaatgatga aagatgacgt 108300 tcaatgggct actactcaat atgctgaagt taataaagca ttagttaaag ctttcattga 108360 tgacaagaaa gtatgtgaag ttgattgtcg aggataatat gcatattgtt ttatttaaac 108420 ctactccata tagtgttagg aaaaatactc aattcaaagc acttatcgca gatacgtggg 108480 aattggtatt agatattcca gcagaagaaa gccctccatt tggtcgagtg gaatttatta 108540 agtttgctgt gcgccctacg aagcgacaga ttcgccaatg caaaagatac tttcgtaaaa 108600 tcgtcaagtt agagaaacag tttgtaacgt gtgattatac agaagtttta aaataactgt 108660 ttacttttat tggaaaatga gatattatat aaacataaac tactgaggag attattatga 108720 aaaaatttat ctttgctgca atttttgctt tatcttcttg cgctgctcag cctgctatgg 108780 cgggttatga caaagatttg tgtgaatggt ctatgactgc agatcagact gaagttgaaa 108840 ctcaaattga agcggatatt atgaatatcg ttgagcgtga tcgtcctgaa atgaaagctg 108900 aggtgcaaaa acagcttaag tctggtggtg taatgcagta taattatgtt ctgtattgcg 108960 ataaaaactt taataataaa aatatcatcg ctgaagtggt aggtgaataa ttagaggtta 109020 atatgtatag ttctgaattt ccatatttaa aaatggaaaa aatttcatac gattttatag 109080 atgaaaaggt ttattacagt tttcatgagc cgcgttttaa cagtgaagtc ggttttattg 109140 tagtaaaaga taatttcatt ttaaaaatat attcggcatt aaaggatttt cactacgaaa 109200 atattaacct aaaatttgat aaagaaaacg ttcgtaattg cgcagtaaca attacaggaa 109260 ataaaggtac atgtgttatg ctatctgatg aaattaatga tttgctaaat aatgcagaaa 109320 agattaccat tccatctatt gatgaccaaa tttttaatgc ttttatgaat agaggttaat 109380 atgaaaacat ataaagaatt tattaaagaa gatatggtag ctggagattc aggtggtaat 109440 cctgaaaata tctctactgg aacaacgtca ggtgctgtag taaataaagg tcctgaacag 109500 attcctaaaa agaaaaaaga ggaatctaaa gaaaaagaag agtaaaaatg tcatcaatac 109560 cttggattga taatgagttt gcgtatcgtg cattagctca tttacctaaa ttcacacaag 109620 taaataatag ttcaactttt aaattgcggt ttagatgccc tgtttgtgga gattcaaaaa 109680 ccgaccagaa taaagctcgt ggatggtatt atggtgataa taatgaagga aatattcatt 109740 gttataactg taactatcat gcaccaatag gaatatattt aaaggagttt gaacctgatt 109800 tatatcgtga gtatatcttt gaaataagaa aagaaaaagg taaaagtcgt ccagtagaaa 109860 aacctaaaga acttcctaaa caacctgaga agaaaataat taaatctctt ccgtcatgtg 109920 ttagattaga taaactggcg gaagaccatc caattataaa atacgtaaaa gctcgttgta 109980 ttccaaagga taaatggaaa tatctttggt ttacaactga atggcctaaa ttagttaata 110040 gtatagcacc aggaacatat aaaaaggaaa tacctgagcc tcgtcttgtt attccaattt 110100 ataatgctaa tggaaaagct gagtcttttc aaggacgcgc attaaagaaa gatgctcctc 110160 aaaaatatat caccatcaaa gcttatcctg aggcaacaaa aatctatgga gtcgaacggg 110220 ttaaagatgg tgatgtatat gttctagaag gacctataga ttcgcttttt attgaaaatg 110280 gtatagctat tacgggcggt caattagacc tagaaattgt tccatttaaa gatagacgtg 110340 tatgggtttt ggataatgaa cctcgtcatc ctgacactat taaacgaatg actaaattgg 110400 ttgatgcagg agaaagggtt atgttttggg acaaatctcc ctggaaatca aaagatgtta 110460 atgatatgat tagaaaggaa ggtgcaaccc ctgaacaaat tatggaatat atgaaaaata 110520 atattgccca ggggttgatg gctaaaatgc ggttatctaa atatgctaag atttaaatta 110580 acccaactaa agcaaatgct aaatctacga atgtatcaag agtaactact ggaatattaa 110640 taccatgtgc aattgcaatg ggtgataaaa taaagttcca gagtaaaatt cctaccatag 110700 cagaaatagt aaaagctata cgttttttat taccttttat ggcatcaaca agtgccatta 110760 atttttgtgc catgtgtcct cctttaaatt aatatttatc ataaatttgt ttactttctc 110820 agcttgttat gatattataa aataaattgc tgaggatgat atgatgatta ataaaattgt 110880 gcatgaaatg gctttaaatg gagattcata taaaatatct gccgtagttg aaaatttcat 110940 acttaataaa gtaaaagaat atttcactga ttgttcagtt agttatcaag aaaaaatggt 111000 tttaaccgat gatactgaaa aatcaaataa tttgttttgt tctaatttta taactaagaa 111060 gcgtactaga agatttgata ttgttatttc tcgcaacggt aaaaagcata taattgaaat 111120 taaacatcaa gttggtggag gtacagctat tgattcggtt ggaatatatt tagaagataa 111180 agagaaatta aaagaataca caaaaactga aaaccctgtg tcattgatga tattagattt 111240 tttgccatgc ggatattatc cacgtaataa atggacaaaa agagaatcat ttactgataa 111300 tccaactatt caggcgaggt ttaatgaata tgctaaatca caaaacgtgt tagtattact 111360 atcaaataca tatgatgaag aattgtataa ttcatttttt gctgcaataa atgagagaat 111420 ataatgctag gagctatcgc gtatacaggt aataaacaat cattattacc tgaacttaag 111480 cctcactttc caaaatatga cagattcgtg gatttatttt gtggaggttt atcagtgtct 111540 ttgaacgtca atggtcctgt attggccaat gatattcaag aaccaattat tgaaatgtat 111600 aagcgtctta ttaatgtatc atgggatgac gttttaaaag taataaagca atataaacta 111660 tcaaaaacat caaaagaaga gtttttgaaa ttacgtgaag attataataa aactagagat 111720 cctcttttac tttatgttct tcattttcac ggatttagta atatgattcg tataaacgat 111780 aaaggaaatt ttactactcc gtttggaaaa agaactataa acaaaaatag tgaaaaacgc 111840 tttaatcact ttaaacaaaa ttgtgataaa ataatcttta gttcattgca ttttaaagat 111900 gtcaaaattc tagacggcga ttttgtatat gtggaccctc catatcttat aacagttgct 111960 gattataata aattttggtc agaagaagaa gaaaaagacc ttttaaatct tttagattct 112020 ttaaatgaca gaggaataaa atttggactg tcgaatgttt tagagcatca cggaaaggaa 112080 aacactcttc ttaaagaatg gtctaaaaaa tataatgtta agcatcttaa taaaaaatac 112140 gtctttaaca tatatcattc caaagaaaag aatggaactg atgaagtata tatttttaat 112200 taattgctta cataatcaaa tgatataatt atttaactta ttaatgaatt gaaaggaaaa 112260 ataatggctt actttaacga atgtagtcaa ctgatcgaag gcgctgataa agctcaaaat 112320 gaatactggg atattctcgg tgatgaaaaa gacccgctgc aggttatgct tgatatgcag 112380 aaatctttgc aggttcgttt agcaaatgac cgcgaatact gctaccatcc agataaatta 112440 gaaactgccg gtgatgttgt ttcttggatg cgtgaacaaa aagactgtat tgatgatgaa 112500 ttccgcgaac ttctgacttc tcttggtgaa atgtcacgtg gtgaaaaaga tgcatctgca 112560 gtttggaaaa aatggaaagc acgttatatt gaagcgcaag aaaaacgcat tgatgaaatg 112620 tccccagaag atcagcttga aattaaattt gagcttgtgg atatatttca tttcgtatta 112680 aatatgtttg ctggccttgg aatgaatgcg gaagaaatct ttaaacttta ttatttgaaa 112740 aataaacata attttgaacg tcaagataat gggtattaaa caaaaatttt atagaacata 112800 tatagtaaaa gttaggacgc cgaaaggcgt cttttggtac gctgggaaac atgaatcatt 112860 tattgtaaat ccatatgatg ataaatatcc gggttcaggc aaaatattat ggaacatata 112920 tcgtaagtat ggttttaatt ataaaatacg ttggtcaaaa tgccatggtt ctagagaaaa 112980 atcatatgaa gttgaacgcg agctaatatc tgcattaaaa cgtaaacacc cagatacttg 113040 cattaatatt tctcctggtg gtcagggtgg agaaggaaga aaatggactg agcaacaacg 113100 attagaacat aaacttagat taaacaaccc tgaaacaaaa actcggatga agaattcaca 113160 acgtatagcc caaaatagag cagaacgaaa agctcggcaa tctgaagtaa tgaaaaagtt 113220 ttattcgaat ggcggaaata aaaagatttc agaaggaact tcaagggcgc aaagaaaagc 113280 accgcattgg catgaaccgc ttaaaagcga aatacacgag ttatgggttt ctttaggtaa 113340 accagcaaca ggcccggttg taaaggcgct taaaggaaaa tatgatgtaa caagttcagc 113400 tcttaagaat ttaatttact tattcagaaa agaagatgta taaataatca tgtaatttaa 113460 ataaaaggag aattacatgg ctagtactcg cggttatgtt aatatcaaaa catttgagca 113520 gaaattagat ggaaataaga aaattgaagg aaaggaaatt tccgtagctt tccctcttta 113580 ttctgacgtt cacaaaattt ctggtgctca ttaccagaca ttcccttcag aaaaagcagc 113640 atattctaca gtatatgaag aaaatcaacg tactgaatgg attgctgcaa atgaagattt 113700 gtggaaagta actggtgaat aaattcaagg actccttcgg gagtcctttt tcattttacc 113760 ggtttacttt ccaaaatgag tatggtataa tagaattatc ttatagagga gagcgctatg 113820 ttaaatcgct ggattaaacc aaatgaagac ctccagaagg ttcttgataa agctatctct 113880 gataaatggg gcatgaaaag ctgggattgt gatatcgtca cgcattcatt catgatgcat 113940 gcagatggtt cagtcgagtt caatgctgaa atgcgataca ctgactgggg tggatttcaa 114000 agaatcgaat ttcaaagagg ctttttgtaa tgtttatctt taattggttt aaaagtttct 114060 ttacggattt tttctctacg actcctgggg aaggtgtagt tcctatttca aatgactacc 114120 ttcctttaac tgtagttgag tatgtttata tgggagatgg aacagtagaa gcagttacta 114180 tgacttatga agaagcccaa gaatattata aaaatccttg gcgctggtca gtacctacgt 114240 ctgcaccatc taatacacag tctagttctg attcatatga tactaatgtt cctgttcatg 114300 tatgggcggg cgattcatgt ggaagttctt gtgattctag ttgttcatct acatcttgtg 114360 attgaggaaa attatggaag cgattttgtt tgaaatgtat attagcagta atagcatgtc 114420 gtttgctaaa gatgttccaa ttaccgtagc agtaatgatt gataagggtt attgtgaccc 114480 aatgtatctc gtagaaaatt tcgtttcaat gccagttcca gaagatgctg aaataaaact 114540 taaaaagatt agtattattg aaactgtgcc aaatattccg tttagagcaa ttgaagcatt 114600 tactaaatcc gaatacatta atgttagcgc agaacaatat aatgataaac ctttatcatt 114660 ttattcatac gattcagtat atagttggaa aatagataaa ggaaataaat ttataattgc 114720 gagtgaagac gctttatcat attttatttc ttctgtatgg aatagtttac atccaaattt 114780 gctaaaaatt catgaatttg acgatgctcc tactgtcgtt ttaggtaaaa caaatgaaag 114840 ttctgaagaa aatacttgaa tggtttagta gaccaaactc aatgtacatt gatgatggtt 114900 gggttgaaca agcaaataaa gaaatgcaga acgaatcgga agaatggatg aaatcaatga 114960 ttagtgctga gaaagaaaag aaattagaac gctcagcgct taaattgatg agagacatct 115020 atggtgataa atcatgaaca aagatatgac attggaagag gctaaagcaa aagcaaatga 115080 agcactggat ttgcttctta aaattggcag taaaatgatg gaagaaaatg agaaatatat 115140 ccaggaaaac aaaattcccg atggtccatt agtaggcaaa aggaaatcac atgattgaag 115200 tagcaaaaca ttattcaata gaatttatgt ctaaagaagg taaatcagta aatacacttg 115260 ataaaaaatg ctcattaatt attcctttag cagaaaatcc ggatttttta attgaagata 115320 taaaagaaag aaaatatcca gaaaatgtta ttctaattat aaaacatact gaagatattt 115380 tgcaggatac tgattcaccg ttttcttctt ctgaagcttt aattattaaa ggctataaaa 115440 gagttcatga atatggtctt tttgacctgt ttgaagacga taaagttaaa ttagcgagtc 115500 aaccttcaaa aagtaaaaca ttcattattg aagatattaa agatataaat gcatttgtta 115560 agatggtctg ggctcatttt gacgttggac tacgctggag aatgtccgaa gaagaaagaa 115620 agattattga agctaatcgt caatttggtt tttatcgcta ggaattaata tggatttatt 115680 tgagatgtta gaagataatc attctacgaa tatccagaat gattctagtg attataagaa 115740 agagtaccgt atagtattac agaattatgg aattgaagcc ccagatgctc ttctagaaga 115800 actagcttca taccatcttg accctccgcc ctgggctccc tgggcaaaat aattcaaaaa 115860 gttgtttact ttcctttcta acgatgatat gatagcttct gaagtatacg gaggctatca 115920 tgattattaa tcttgcagat gttgaacagt tatctataaa agctgaaagc gttgattttc 115980 aatatgatat gtataaaaag gtctgtgaaa aatttactga ctttgagcag tcaattcttt 116040 ggcaatgtat ggaagctaaa aagaatgaag ctcttcatca gcagttgaat aaaatcgtta 116100 aaaagcattt aactaaatca ccttatcagt tatatcgtgg tatatcaaaa tcgacaaaag 116160 aacttattaa agatttacaa gttggagaag tgttttcaac gaacagagta gattcattta 116220 ctactagttt gcacacagca tgtggttttt cttatgctga atatttcact gaaacaatac 116280 ttcgtttaaa aactgataaa gcttttaatt attctgacca tatcagcgat attatacttt 116340 cttctcctaa tactgagttt aagtacacgt atgaagatac tgatgggcta gattcagagc 116400 gtactgataa cttaatgatg attgtacgtg aacaagaatg gatgattcca attggaaagt 116460 atagaataac ttctatttca aaagaaaaat tacacgattc atttggaaca tttaaagttt 116520 atgatattga ggtagttgaa tgaaatactc agtaatgcaa ctaaaagatt ttaaaataaa 116580 atcaatggat gcatcggtgc gtgcttctat tcgtgaagaa ttactttctg aagggtttaa 116640 tttatctgaa attgaacttt taattcattg tattactaat aaaccagacg atcattcttg 116700 gttaaatgaa ataatcaaat ctcgtttggt tccaaacgat aaacctcttt ggagaggtgt 116760 tccagctgag actaagcaag tattaaatca aggaattgat attattacat ttgataaagt 116820 cgtatcagct tcatatgata aaaatatagc tctacatttt gcttctgggt tagagtataa 116880 cacacaagtt atttttgaat tcaaagctcc tatggtattc aatttccagg agtatgctat 116940 aaaagctcta cgctgtaaag aatacaatcc aaactttaag tttccggata gccatcgtta 117000 tcgtaatatg gaattagttt cagatgaaca agaagtaatg ataccggctg gaagtgtatt 117060 tagaattgca gatagatatg agtataaaaa gtgttcaaca tacactatct atactcttga 117120 ttttgaagga tttaatctat aatggaagga cttagattca ttataccatg aaagttttaa 117180 agcatttttc ataaagttgt ttacaagtta aagtaaaaat gttatagtat aagtagttaa 117240 ccgtccgtaa gatgtgagaa aaatatgaag ctgtctaata accaaattcg taaaattaaa 117300 cgtcgtctag agcatactca ggcatctgca aaaagacgtt ctaaagattt taacttagac 117360 ttcaattaca ttaagaacat tttagatcag aaagtttgtg cttactcagg agaacctttt 117420 gataatcgta ttgaaggaga gaaattatca ttagaacgtt ttgataataa cgttggatac 117480 attaaaggga atgttattgc agtaaagaaa aagtataata catttcgttc tgattatact 117540 ttagaagagt tgattgaaaa gcgtgattta tttgctttgc gaattggtcg ttcatctgcg 117600 aaaaaagttc ataaactaaa tttagatgaa aagaaatggg ctaaaatcaa aaagacttat 117660 aatcaaatta aagctataca gaaaaaacgt gaaaaccgaa ttgaacacat ttctcagctt 117720 tctaaatcaa aacagacctc tgacattaag ctaagaatta tagcactcaa agctcgtatt 117780 gatggttctc gtatagcaga aggcgctgaa gttgttaaat tgaacgttct tcttaaaggc 117840 tcggattgga aaaccgtgaa aaagttgtca gaagcagaaa tgcaatatga tatgtgtgat 117900 aaaattattc aaggtgtaga gcggtatcaa aacttgtctt ttattgataa acttaaactg 117960 aaaagaggat acccgctaaa ttgttcaatt tttaaactta tccgaggata atatgtttta 118020 tgtatatgcg atagtgtata gagataaaga tggatttgtg gtgcctgttc ctcttgatga 118080 acatcgtcct gctgtatttt ttgaaaggga gattgctgat aaagtattta ctactcttaa 118140 agagcagtat caactagctt taggtatggg aattccgaga ttagttgaga ctccacgcaa 118200 gttttggttt aataaaatag aagttaaaca cgttaagcct gatgtagaca cgcaaagatt 118260 atatcggcga attttagata ctgggcgtat tgttagtata ccaattgcag ggaatttacg 118320 atgacatttg atgatttgac tgaaggccag aaaaatgcct ttaacactgt tatgagggct 118380 attaaagaaa agaaacatca tgtaactatt aatggacctg ccggtactgg taagactact 118440 cttactaagt tcatcattga agctttaata tctacgggcg aaactggcat tattttagca 118500 gctcctactc atgcggctaa aaagattctt tcaaaactgt caggaaaaga agcgagtact 118560 attcacagta ttctcaaaat taacccagta acatatgaag aaaacgttct ttttgagcaa 118620 aaagaagtac cggatttggc taaatgcaga gtattaatct gcgacgaagt gtcaatgtat 118680 gatagaaagc tatttaaaat tctgctttca actatcccgc cgtggtgtac tataattgga 118740 ataggtgata ataagcagat tagacctgtt gacccaggag aaaacactgc ttatatcagt 118800 ccattcttta cacacaaaga tttttatcag tgtgaactca ctgaagttaa acgcagtaat 118860 gctcctatta ttgatgtagc tactgacgtt cgtaacggta agtggattta tgataaaatt 118920 gttgacgggc atggagtacg tggatttact ggtgataccg ctttacgcga ttttatggta 118980 aattattttt caatcgtcaa atctctagat gatttgtttg aaaatcgcgt aatggcattt 119040 acgaataaat ctgttgacaa gttaaatagc attattcgta aaaagatttt tgaaactgat 119100 aaagatttta ttgtcggtga aattattgta atgcaggaac cattaattaa aacatataaa 119160 attgatggaa agcttgtgtc agaaattatt tttaataacg gacaattagt tcgtattata 119220 gaagcagagt atacatcaac ctttgttaaa gctcgtggtg ttcctggaga atatctaatt 119280 cgtcattggg atttaacagt agaaacttac ggcgatgatg aatattatcg tgaaaagatt 119340 aaaataattt catctgatga agagctgtat aagtttaact tatttttagg taaaacggca 119400 gaaacttata aaaattggaa taaaggcgga aaagctccgt ggagtgattt ttgggatgct 119460 aaatcacagt ttagtaaagt gaaagcactt cctgcatcaa catttcataa agctcagggc 119520 atgtctgtag accgtgcttt tatctatacg ccttgtattc attatgcaga tgctgaacta 119580 gctcagcagc ttctttatgt tggtgtaaca cgtggtcgtt atgatgtgtt ttacgtatga 119640 ttaaattcga ggaagctatt cgtggaaata actaaagatc agttttatct gctccaagat 119700 aaagtaagcg aaatttatga aatagcttat agtaaaaatc gcaaaaacgt gaaaattgaa 119760 tcaggcaagt taatgcttca attggaagaa attgaacgag atttgattgc gttagaattc 119820 ttttgcggtg aagtgaaaac tgttacaatc agtgattaca ttttaggtga aattagccat 119880 ctttataagg cggttattaa tgattgaatt aagttggtgt cagtttaaat ttcttatgac 119940 aaatgttaaa gctgtcattg agaaaaattc tggtcctgaa aatattacta ttcgcgaaaa 120000 agctttaaag ataatataca gtcttgaaga gatgcaaaaa gatattgaat ctatggcaaa 120060 atttattgat gaacctatta ataaagttta tattcaagac tatactgtag gtcaaattcg 120120 cgatttagca aggaaaattt aatgtttgat tttattatag attttgaaac aatgggaagt 120180 ggtgaaaaag cagcggttat tgatttagct gtaattgctt ttgaccctaa tccagaagtt 120240 gttgaaacat tcgatgaact agtttcacgt ggcattaaaa tcaaatttga tttaaaaagc 120300 caaaaaggac atcgtctttt tactaaaagc actatcgaat ggtggaaaaa tcagtctcct 120360 gaagctcgaa aaaatattgc accatcagat gaagatgtaa gcactatcga tggtattgca 120420 aaatttaatg attacatcaa tgcgcataat atcgatcctt ggaaatctca aggctggtgc 120480 cgtggaatgt cgtttgattt tccaatttta gtcgatctca ttcgcgatat tcaacgcctt 120540 aatggtgtat ctgagaatga gcttgacaca tttaagttag aaccatgtaa attctggaat 120600 cagcgtgata ttcgtaccag aattgaagca cttctgcttg ttcgtgatat gaccacatgc 120660 ccccttccaa agggaacttt agatggattt gttgcgcata attctattca tgactgtgcg 120720 aaagacatcc tgatgatgaa gtatgctttg cgatacgcta tgggtcttga agatgctccg 120780 tcagaggaag aatgcgatcc tctgtctctt ccaacaaaac gataaaaagt tgtttacttc 120840 ctcggttagt tgtggtatta taacaccata gctactgagg ataataaaat gaaaatttat 120900 cgtgttgaat catcgtttag tattcttaat aatgaagatg ctataacaat acgtcgaaat 120960 ctttgcgttc aaataacgcc atacaggagt ataatagatt catggagcga agagtggcta 121020 ttacacgtag gttatgacag acctaatttt atgcatcata gcgataataa taaaagaatt 121080 cctttaccac acgaagataa actattagtt aaaaacgcta atatagtaat taatactaag 121140 ttcaagaaag attatgttgg agtagaatat catattcctg gatggtttat agctctttat 121200 cattttgctt tcgctagcga atatgatatg atgagatggt tcacacgaga agagcgggaa 121260 gaattatctt ctaaaggatt ttatctcgct gtatacgaag ttcctgaaga tgaagttatc 121320 attggtgggc accaagtaat gttccgcaaa tcgcatgctg aactcgtaga ttttattgaa 121380 atgagataat tatgaaattt aattataatc ctgaatacac accgaatcct gtagataaac 121440 tgattgattt tgatgttgta agcacttatg tatgccctat taaaccactg gaaattaagg 121500 aacctactat gactaccgct attgaaatcg gcaaaaccta caaactggtt gaacctaaaa 121560 ttaaaactaa tgccttgatt tctggtcata aaactctgac tgatgttttt ggcgaaggcg 121620 aatttattgt tgaagaattt gccaaaagtg agtggtttga caaatcttac gtcatccacg 121680 gtcgccggtt agataataac aaaataaaga aaaacctggt ttatgaagat gagttcatcc 121740 tgttccaaga agttgaagaa caagacccta cagacctgtt gtgtgctgct gtgtctatcc 121800 gtcgtccttt tgataatcct atctgtggtt gggtaacaga ccagtggatt gaagatggtg 121860 ttgagcttct gaacgttgtc catgcaggag attttagtgt agtacctcgt agtgcggtgg 121920 tagctatttt gaattaatag tttacaaact cttgggacca gagtataatg gtcctgtgga 121980 gtataaaatc tttttaacaa gtgagagata actatgatta ttaatattgg tgaaattgct 122040 cgtgtatctg ataaatcccg ttctaaagca gcaggaaaat tggtcgaaat tgtaagcatt 122100 cagcttaaac acggcgttaa agatgaagat tctgaagtaa aagtgcgtat cattcctaaa 122160 gatggaaagt ctaaacctca gtttggctat gttcgtgcga aatttcttga atctgcgttt 122220 ttgaaagctg ttcctgctaa aggaattgaa acgattgata cttcgcatgt aggtgtagac 122280 tttaagtgga aactcggtca ggctattaag ttcgttgctc cttatgaatt cgaatttact 122340 gaagatgagg aaggggttgt tcaaaaacac catactcgcg ctatgtgtgc atacattact 122400 gatcaatggg tagaagatgg tgttaagctg tacaatgcgg tatttttagg aacatacaaa 122460 gtcattcctg aaagttggat taaacactat agcaaagctc gctgtgcata aagtttaaaa 122520 ttttttcata aaactatata catcagtagt tgattgtggt actatatcaa tatcaactac 122580 tgatacagaa aacaacttga agaataaaat ggataattcg ttaaaggtgc gctgatactc 122640 ttctgaaacg catcgctcca atgttcaatt aatgaggaaa ttatgatgaa acgtaaaatt 122700 gttcagaatt gcactaatga tgaatttgaa gatgtattat tcgatccaga tttggtagta 122760 gttcaaaagg aacatactag caagtttact cacttgactt ctgtttatgt gtatgagaaa 122820 gtcggtgata aacaaccaat ttatggtgta tttcgtgaaa ttactgaaaa tggcacaact 122880 tattggaagg aaatttatta atggctatta aatttgaagt taataaatgg tatcaattca 122940 aaaataaaca agctcaagaa aattttatta aagaccatac tgataacgga atctatgcgc 123000 ggcgtttagg tatggaacct tttaaaattt tagatgttga ctattacggg cgtcctacta 123060 aaattgtgac atctactggc atagttggat attcatcacg aggtacagtc attgacgaaa 123120 actttatctg gctttctagc aacgaagccg agttctttaa tgaagtggaa aatccatatc 123180 aggctactga agagcaggaa gaccctgcac cagtaactga accatctgaa ttcccggtaa 123240 tgaaagttac tattgaaaat aatgaacaag catggtcctt gtatcagatg ttgaaagctc 123300 actttaagga ataattatgc cgctttatga ttataaatgc caatccgaag actgtgcaaa 123360 agaatacgaa aaaatcaaga aaatttctga aagagatact gatgtatgtc ctgattgtca 123420 tcggattgct attcggttag tttccgctcc taagcatgtg aacggtggat tttacgactt 123480 acttaaaggg taattatgaa atatgttaat cgttctatcg cggcattagt attagcagta 123540 tctttagtag gatgtactga tgctgataat gcaaccaaag ttttgtcttc aagcggtttt 123600 actaatattg aaatcactgg atataattgg ttcggttgct ctgaaaatga tttccagcat 123660 actggatttc gtgctattgg acctactggg cagaaagtag aaggaacagt atgttctgga 123720 ctattcttca aggattcgac tattcgtttt aaataaaagg ccttcgggcc tttagcttta 123780 tgattattgg agtataatat tcccgaaacc aaacgaggat aagtgatgat taagaatgaa 123840 attaaaattc tgagcgatat tgaacatatc aaaaagcgta gtggcatgta tattggctct 123900 tctgctaatg aaatgcatga gcgctttctg tttggtaaat gggaaagtgt tcagtatgta 123960 cctggtcttg ttaagcttat tgatgaaatt atcgataact cggtagatga aggtattcgt 124020 actaagttta aattcgcgaa taaaattaat gttactatca aaaacaatca agtaacagtt 124080 gaagataacg gtcgtggtat tccacaagcg atggttaaaa cacctactgg tgaagaaatt 124140 cctggtcctg ttgccgcctg gactattcca aaagcaggtg gtaacttcgg tgatgacaaa 124200 gaacgcgtca ctggtggtat gaacggtgtt ggttctagtt taactaacat tttttctgtg 124260 atgtttgtcg gtgaaactgg cgacggtcaa aataatattg tagttcgttg ttcaaatggc 124320 atggaaaata aatcatggga aactattcct ggaaaatgga aaggaactcg tgttactttc 124380 attcctgatt ttatgtcatt tgaaactagt gagctatctc aagtttatct tgacattacg 124440 ctagatcgtc tccagacact tgcagtagtt tatcctgata ttcaatttac ctttaatggt 124500 aaaaaggttc agggtaattt taagaaatac gcacgccaat atgatgagca tgctattgtt 124560 caagaacaag aaaattgttc tattgcggtt ggtcgttcac cggatggttt tcgtcaatta 124620 acgtacgtca ataacattca tactaagaat ggtggccatc acattgattg tgttatggat 124680 gatatttgtg aagaccttat tccacaaatc aaacgcaagt tcaaaattga tgtaactaaa 124740 gcacgtgtta aagaatgttt gactatcgtt atgtttgttc gtgatatgaa aaacatgcga 124800 tttgattctc aaactaaaga gcgtttgact tctccttttg gtgaaattcg tagtcatatt 124860 caacttgatg ctaaaaagat ttcacgtgct attttaagta atgaagcaat tttaatgcca 124920 attattgaag ctgccttggc tcgtaaattg gcggcagaaa aagcagcaga aactaaagca 124980 gctaaaaagg cttctaaagc taaggttcat aaacatatta aagcgaatct ttgcggtaaa 125040 gatgctgaca ctactctttt cttgactgag ggtgattctg ctatcggata tcttattgat 125100 gttcgtgata aagaacttca tggtggttat ccattgcgcg gtaaagttct taacagttgg 125160 ggcatgtcat atgctgatat gcttaaaaac aaagaactgt ttgatatttg cgcaatcact 125220 ggattagttc ttggtgaaaa ggctgaaaac ttgaattatc ataatattgc tattatgacc 125280 gatgctgatc acgatggtct aggaagcatt tatccttctc tgctcggatt ttttagtaat 125340 tggccagaac tgtttgaaca aggaagaatt cgcttcgtca aaactcctgt aatcatcgct 125400 caggtcggta aaaaacaaga atggttttat acagtcgctg aatatgagag tgccaaagat 125460 gctctaccta aacatagcat ccgttatatt aagggacttg gctctttgga aaaatctgaa 125520 tatcgtgaga tgattcaaaa cccagtatat gatgttgtta aacttcctga gaactggaaa 125580 gagctttttg aaatgctcat gggagataat gctgaccttc gtaaagaatg gatgagccaa 125640 tagtttactt taccacaagg atgtggtata attaattggg caaatgagga tattgaaatg 125700 aaatcatata aagtaaattt agaacttttt gataaagcag ttcatcgaga atatagaatc 125760 attcaacgct ttttcgatat gggagaagct gaagagttta aaaaccgctt taaggatatt 125820 agagataaaa ttcaatccga caccgcaact aaagatgaac tactagaagt tgctgaagtt 125880 attaaacgca atatgaatta atgaggaaat tatgattatc accactgaaa aagaaacaat 125940 tcttggtaat ggttctaaat caaaagcatt tagcatcaca gcatctccta aagtatttaa 126000 aattctgtca tctgacttgt atacaaacaa gattcgcgca gtagtccgtg aattgattac 126060 caacatgatt gatgcccatg ctctcaatgg aaatcctgaa aaatttatca ttcaagttcc 126120 aggacgatta gatccgcgat ttgtttgtcg agattttggt ccgggtatga gcgattttga 126180 tattcagggt gatgataatt ctcctgggtt gtataattca tactttagtt catctaaggc 126240 tgaatctaac gatttcattg gcggatttgg tttaggttct aaatctccgt ttagttatac 126300 tgatacattt agtattactt catatcataa aggcgaaatt cgtggttatg tagcttacat 126360 ggatggcgat ggtccacaaa ttaaacctac atttgtaaaa gaaatgggtc cagatgataa 126420 aactggtatt gaaatcgtag ttccagttga agaaaaagac tttagaaact ttgcttatga 126480 agtttcttat atcatgcgac cattcaaaga tttagctatc attaatggtc ttgaccgcga 126540 aattgattat tttccggatt ttgatgacta ttacggcgta aatccagaaa gatactggcc 126600 agatcgtggt ggattgtatg ctatctatgg tggtattgtt tatcctatcg atggtgttat 126660 tagagaccgt aactggttaa gcatccgtaa tgaagtgaat tacattaagt ttccaatggg 126720 ttcacttgat attgctccat cacgcgaagc tctttcacta gatgatcgta ctcgtaaaaa 126780 tattattgag cgtgttaaag agcttagtga gaaggcattc aatgaagatg taaaacgatt 126840 taaagaatct acatctcctc gtcatacata ccgtgaattg atgaagatgg gatattctgc 126900 tcgagattat atgattagta attcagtcaa atttacgact aaaaatctgt catataagaa 126960 gatgcaaagc atgtttgaac cagatagtaa gctatgcaat gcaggagttg tgtatgaagt 127020 aaatcttgac cctcgactga aacgcattaa gcaaagtcat gaaacttcag ccgttgcatc 127080 aagttatcgt ctgttcggta ttaatacaac aaaaattaat atcgttattg ataatattaa 127140 aaatcgcgtt aatattgtcc gtggattagc gcatgcgtta gatgataaag aatttaataa 127200 cactttgaat attcatcata atgagcgtct tttgtttatt aatccggaag tagaatcgca 127260 gattgattta ctccctgata ttatggcaat gtttgaaagt gatgaagtta acattcatta 127320 tttgtcagaa attgaagctt tagttaaaag ttatattcca aaggcagtaa aaagtacagc 127380 tcctcgtcct aaagctgcta ctgcgtttaa gtttgaaatt aaagacgggc gctgggaaaa 127440 agaggaactg tttacactta cgtcagaagc agatgaaatt actggttatg tagcatacat 127500 gcatcgttct gatattttct ctatggatgg cactacatcg ctttgtcatc catctacgag 127560 tattttgact cgtatggcta atcttattgg cattaatgaa ttttatgtta ttcgtccgct 127620 tttacagaaa aaggtaaaag aacttggtca gtgccaatgt atttttgaaa ctctgcgtga 127680 tttatatgta gatgcttttg atgctgtaga ttatgataag tatgtaggtt attcaagttc 127740 agctaaacga tatattgata aaattatcaa gtatcctgag ttagatttta tgatgaagta 127800 cttcagcgta gatgaagttt ctgaagaata tacacgactc gctaatatgg ttagttcatt 127860 acagggtgta tatttcaacg gtggaaaaga taccatcggt catgacatct ggacagtaac 127920 taatcttttt gatgtattat caaataatgc ttcaaaaaat agtgataaaa tggttgctga 127980 gtttaccaag aaattccgta ttgtttccga cttcatcgga tatcgcaatt ctttaagtga 128040 tgatgaagtt tcccaaatcg ctaaaactat gaaggccctt gcggcctaat aaggaaaatt 128100 atgtacaata ttaaatgcct gaccaaaaac gaacaagctg aaattgtcaa actgtattca 128160 agtggtaatt acacccaaca ggaattggct gattggcaag gtgtatcggt ggacacaatc 128220 cgtcgtgttt tgaaaaatgc tgaagaagct gagcgctcca aagttaccat tagcggtgac 128280 attacagtta cagttaagag tgatgcagct attgctccag ttgctaaatc tgacattatt 128340 tggaatgcat ctaaaaaatt catttcaatt accgttgacg gcgtaactta taacgcaact 128400 cctaatactc attcaaactt ccaagaaatt cttaatctgc ttgttgcaga taaattggaa 128460 gaagctgcgc aaaaaattaa cgttcgtcgc gctgttgaaa aatatatttc tggcgatgtt 128520 cgaattgaag gtggaagctt gttctatcaa aacattgaat tacggtctgg tttagttgat 128580 cgtattctcg attcgatgga aaaaggtgaa aactttgaat tttattttcc gttcctggaa 128640 aatctgctgg aaaacccaag ccaaaaagca gtatctcgac tctttgattt cttggtagca 128700 aatgatattg aaattacaga agatggttac ttctatgctt ggaaagtagt tcgcagcaat 128760 tactttgatt gtcactcaaa tacctttgat aacagccctg gtaaagtagt taaaatgcca 128820 cgtactcgcg tgaatgacga tgatacgcaa acttgttctc gtggtctgca tgtgtgttct 128880 aaatcttata ttcgtcactt tggcagttca accagccgag ttgtaaaagt taaagtgcat 128940 cctcgcgatg tagtatcaat tccgattgat tataacgacg ctaaaatgcg tacctgccaa 129000 tacgaagtag ttgaagacgt tactgaacaa tttaaataag ggcttcggcc cttaactaag 129060 gaaaattatg ttaggttatc aagcacgagt aaaagaagaa tacgatcaat taatgctcaa 129120 aattaatgca ctgagcaaat ttttagaaag tgaaaagttt tcaacggtta atgcagttga 129180 gcaagaactg ttgctttcgc agttcatctc aatgaaatct tacgcggatt gtttagagaa 129240 aagaattgca caatttaaat aaaataaggg gcttcggccc ttttgtttta aggaaaaatt 129300 atgatttatt gtatgaatat cggtgattcc gacataaaag agattaaact gcacggaaat 129360 catcacgcta atattgtgta ctgtaataaa tttgagtttg gtcacgaaaa acttggggtt 129420 ctttattgta atgatgttat aatcatggat aaaaaggaac tcgatgaact cgaccgtgag 129480 tctttagatg aaaatgatcg aatttattac ggcactctta aagtacataa cgcttatctt 129540 agtggtaaaa aggaaagtct aaatgcagaa aacgaatcca ggtttacaga gactatttca 129600 gattccgaca tttaccctat cgaacaatga tttgactagt gaaatgaagg tcaaaattgc 129660 tgatacagca agatactctt taaaacaaaa cccgaaccag gataaagcag aagtcattga 129720 aagatgtcgt atcgctgtgt atgcagagtt ttttgtagca gattggttga gcgggtatgt 129780 taacaaaggc caagaggatg ttaatgaccc gtatacatat gcatgggatg tattggcgca 129840 tccaagatat tgcgggcttc gtgtagaagt caaaacacac caaactgact cacgttggat 129900 ttctgtaaca acaggatgca gcggagagta tccatacggt tctggaataa atctagggcc 129960 cattctgaat catcaggtcg ctgactgtat aattatattc aacactaaag aaattcatcc 130020 aggtgtcatc cagtacactc cgaagttcat cggtgataga gaagaccttc gtaaggttgt 130080 aagaaaaagc aactacaacg gatggtatct ttccatttaa aaattttcac aaaacggttt 130140 acataccaca aggactgtgg tactatacaa ctatcaactg atacggattt ggagaacaaa 130200 atgtttacta cagctgaatt aaaacgagca aaagctaaga aagggcaagg aaaatataaa 130260 gctgaattag ttaaagaact tcagtttgct gaggctgaat tgaattcaat gattattcaa 130320 aatgctccag aaactgaaat tgctcttaag cgtattgcga ataagtgtct tcgtgatgca 130380 atcgtcgatc ttttagcgga ttattgagta aaatgaaatt taaattttat tacgctaaac 130440 ataaaataac tgatgagttt gtgtcagttg aatattctag cgatgatgaa ggtatgattt 130500 atactcgctt agggttatcg cactgggagt ctgatacgcc ttatttttca actcccgaag 130560 aaattacacg tttagttaat ggtcatatga atgaccactg gaatgtaatt ctttcagata 130620 gtcttaaagc ggctattaat agtaatgaaa tgaaaatcgc tgagattgaa ctatgagttc 130680 attatggtgg tgtttcgttt ggttaattag tattccattg atttgtttag catttacttt 130740 tgtgatgagg ttattatgaa aattttgaat tctgtactta ttgcttgtgc gtggtgggtt 130800 gcacaagttt cagcggtagt agttggtatt cacatttatt acgaatattt ttaaaaagtt 130860 gtttacaaga ctgttcctct gtggtactat acaactatca actacggagg aacagaaaat 130920 gaaaaagatt gttaaagcta tatggaatgt agttataata ctagtagttt tgagtatatt 130980 cccaatcgtt ttaatgattg atgtattaaa cgcttacttt ggatttatgt gaggaaaata 131040 tgaagcgtaa acgcagtgct tttacattta ttgaatggtt tttcgataat atttttccag 131100 ctttattcat tttcatgctg atttttgcct taggttcagt tgtagttgga atctatttga 131160 tggcagttgt cggaatggat attcaccaaa atggcttaaa atctgtggtt gaaacaattt 131220 ggaatggtgt aaaataatga atttactaag catttggttt tatattctta tgttttacat 131280 tggtgcaaat ttcccatatt ggatgggatg gtcaacaact gcatttggat tttatactcc 131340 ttgaggtgaa ttatgaaaat ctttaaagat gtaaaagttg gtgaaatttt ttgcttagat 131400 aatggtgatc agttaattcg tatttcgcct cttaaaagca ctagcgagaa aatgacagtt 131460 aacgctactt tagcaaataa cagcaatgaa cgtttctgta ttgaaaatga tactgaaact 131520 tatactgtag aagagttctg ggaattaagc gtcgactgcg acgattaatt tatcggccgt 131580 atgtattcat gcggccttgg agtagaaaat aatttagagg aaattaaaat gaaatatatg 131640 actgttactg atctgaataa tgcaggggca actgttattg gtacaatcaa gggcggtgaa 131700 tgggtttttg gaactccaca taaagacatt ttgtctaacc ctggatttta ctttttagtg 131760 agcaaatttg gtggtccatt tgatagtccg tgtgtatctg cgcgatttta tgtaggtaat 131820 cagcgctcta agcaagggtt cagtgcagtt ctaagtcata ttcgtcagcg tcggtctcag 131880 cttgcgcgta ctattgcaaa taacaatgtt ccatacacgg tattttattt gcctgcttct 131940 aagatgaaac ctctgacgac gggatttgga aaaggtcagt tagctttggc gtttacccgt 132000 aatcatcatt ctgagtatca aacacttgaa gaaatgaacc gtatgttggc tgataatttt 132060 aaatttgttt tgcaggcata ttaatgagca atttccataa cgaacatgtg atgcagttct 132120 atcgtaataa tcttaaaact aaaggcgtct tcggacgcca gtgaggaaaa tatgaatatt 132180 gcaaaattat taggagttat ttcatttatt tgttggatag tagcatgtgt tttaactatt 132240 tgtattgatg ccagcagcgt gttttcacaa gctttagctc agggtatgtg tgcatattta 132300 acatttgtgt tgttatctac taatgattaa gaaaatcttg ggctactcag tagcccttgc 132360 tgctttattg gtagcgctat attatggaat aatgttcggg ttaattcaag tcgtgctttt 132420 catttctgat gttattatgg cactacattc actagtatgg taaatttatg caactgaata 132480 atcgcgattt aaaaagtatc attgataatg aagcattggc ttatgctatg tacacggttg 132540 aaaatcgtgc tattccaaat atgattgatg gatttaagcc agttcaacga tttgttatcg 132600 ctcgagctct tgatttggca cggggaaata aagataagtt tcacaaactc gcttctattg 132660 caggtggtgt agctgacctt ggatatcatc atggcgaaaa ctccgcacaa gatgcggggg 132720 ctttgatggc taacacttgg aataataact ttcctttgtt agatggtcaa ggaaactttg 132780 gttctcgtac cgttcaaaaa gcagcagcaa gtcgttatat ttttgctcgt gtaagtaaaa 132840 atttctataa cgtatataaa gatactgaat atgctccggt acatcaagat aaagaacaca 132900 ttcctcctgc tttctatttg cctattattc ctactgttct tcttaatggt gtttccggta 132960 ttgcaactgg ttatgcaact tacattcttc ctcatagtgt ttcttctgtc aagaaagctg 133020 tactacaagc tcttcaagga aagaaagtaa ctaaaccgaa ggtagaattc ccagaatttc 133080 gtggtgaagt cgttgaaatt gatggccaat atgaaattcg tggaacgtac aagtttactt 133140 cacgaactca aatgaatatc actgagattc cgtataagta tgatcgtgaa acttacgtga 133200 gtaaaatctt agacccgctt gaagataaag gtttcattac atgggatgat gcttgcggtg 133260 agcatggttt tggctttaaa attaaattcc gtaaagaata ctctttgagc gataacgaag 133320 aagaacgcca tgcaaaaatc atgaaagact tcgggttgat tgagcgtcgt tctcagaata 133380 ttacagtcat taatgagaaa ggaaagctgc aagtttacga taacgtagtt gatttaatta 133440 aagacttcgt tgaagttcgt aaaacttatg tccaaaaacg aattgataat aaaattaaag 133500 aaactgagtc agcttttcgt ttagcctttg ccaaggcaca tttcattaag aaagtaattt 133560 caggtaaaat tgttgtacag ggtaaaactc gtaaagaact gaccgaagaa ctttcgaaaa 133620 tcgacatgta ttcttcttat gttgataaac tagttggaat gaatattttt catatgactt 133680 ccgatgaagc aaagaaactt gctgaagaag ctaaagccaa aaaagaagaa aacgaatatt 133740 ggaaaactac tgatgtagtt acagaataca ctaaagattt agaggaaatc aaatgagtcc 133800 attcattggt attacaagcg cggcattagt atctggtagc attttactgg cgggtttagg 133860 tgttgttcct gccatagcag gaggtcttct tgcgttcgga attcaacgtg ttatcatgac 133920 agttatcaca gtcatgcagt aattttaggg agagcctcgg ctctcccttt tttatttcaa 133980 aaattttttc acaaaacggt ttacaaccaa agcatactgt ggtactatac aactatcaac 134040 tactgataca gaattacgga gattagaaaa tgtctaaagt aacttacatc atcaaagctt 134100 ctaacgatgt tctgaatgaa aaaactgctg cgattttaat taccattgct aagaaagatt 134160 tcattacagc tgcagaagtt cgtgaggtgc atccagattt aggtaacgca gtagttaata 134220 gtaatattgg ggtattgatt aaaaaaggcc tggtggagaa atctggtgat ggattaatca 134280 ttactggtga agctcaggat attatttcaa atgcggcaac tttatatgca caggaaaatg 134340 ctcctgaact gctgaaaaaa cgagcaactc gtaaagctcg cgagattact tctgatatgg 134400 aagaagataa agacttcatg ttaaaacttt tagatgaaaa tggatttgtt cttaaaaagg 134460 ttgaaactta tcgtagtaac tatctcgcta ttttagaaaa acgcactcac ggaattcgca 134520 attttgaaat taacaacaat ggaaatatgc gaatttttgg atacaaaatg atggaacatc 134580 atattcagaa atttactgat atcggaatgt catgtaaaat cgctaaaaac ggtaatgtgt 134640 atcttgacat taaacgctcg gcagaaaaca ttgaagctgt aatcaccgta gcatctgaac 134700 tgtgaggaat aaataatgaa caagttagaa attgttaatg aacttcgtcg ttgtgcagaa 134760 cccactcaag aggggtggga catctggtac catggggctt atcttggaac tattgtaaag 134820 attaagactg gtaaatacat gattattcgt gaaagtaaag atgctccagt aggtattcgc 134880 aataatttta tggcagcgat aagttccttt acggatgcag cttacgaaat ttaccttgct 134940 gattataaag aatttcagga atctcaacca gttattcgct caattggcgt taacaaagct 135000 caacagaaaa ctttatggca gcgtattaaa ggatggttta aatgaaaact tttaaagaac 135060 gtttagctct gttagataaa gctttgtcac gtgagactcc agaaagtttg gctgctaaat 135120 tagcttctta cagtggtgaa tacacagaag aagatatttt aaaagaagtt cctgaaatct 135180 gttggcagac cgcatactgg gatgaaaatc aaaagtatca acgaagaatt gtttgcgcag 135240 ccaatcgttt taaattaaaa gatggacgaa ctcttgttat tccaggtgct cgtcattatt 135300 ctaaagatat ggccgaagtt ttagatgtag ttaaacccca attagttact cagcaagttt 135360 gtgatgatga ccaagggttt attgaccaat atagtaatta ttggacacgt gaagaagcaa 135420 tgattattgc aacttatgct ggacaagtac gtattgaacg tggtggtagt gaaaaagaac 135480 tttactctga ggacctttac taatgaatat taaaaagttt caaattgatg gaattatgaa 135540 tcaaattcag gcgctggaat atgccaataa aatgatgtca actaaatggg gaatttatgc 135600 caatgaacca gcatttcagt tctgtaatat ggaattcact aaaaagctcg taggaaaaga 135660 ttatgtatgc ccatttagtt ctccaataaa tggaatgtta aaacctgctt tacgcgatct 135720 ttatattgcg atgaatgaag agatgataaa agagctaaaa cgtcaactga aggtgattca 135780 atttggccag ggaaattaat tcaaaatccg attattttaa ttctctcaat gataaagata 135840 aaaatctaat acggcatttt attgttgaga tgggatatac tgacacacgt gatttaagag 135900 aacatatatt tgaatgtggt gtagctaaaa agttttcatt cacatgcaaa tgcttaagag 135960 aggtaattca gcactatgaa caatttagtc gcaaaacata attttaataa agcttctgtc 136020 cataaagata aaaagaaagc gtttaaagaa tctaatcgca aacagaaaca taaggggaag 136080 gtctatgatt attgattctc aatctgtggt tcaatataca atcaaaattg atattctaga 136140 aaagctatac aagtttctac caaatttata ccactcaatt gtcaatgaat tagttgaaga 136200 attacatctc gagaataatg atttcttggt tggaatttat aaagataact caaaggcggg 136260 atatttttac gtaattccag ctccaggaaa aagtattgat gatgtattaa aaactataat 136320 gttttatgtc catgattacg aaattgaaga ttatttcgaa tgagtcataa tcttgaaatg 136380 gtaatcgagc ataatgtagc tcaggaacgt aagtcgttca aggaattcgt agaaaaaatt 136440 tttgaagaaa ataacacaga ccagtttaca aatcaagcgt ctgatgatat tataataaag 136500 tcaactaatt gagtggtata gttaatgaat aaaaacattg atacagttcg tgaaattatt 136560 actgttgcgt ctattttgat taaattttcc agagaagata ttgttgagaa tcgtgctaat 136620 tttattgcat ttctgaatga gattggagta acgcatgaag gtagaaagtt aaatcagaat 136680 tcattccgta aaattgtttc tgaattaacc caagaagata agaaaaccct catcgacgaa 136740 ttcaacgagg gttttgaggg tgtatatcga tatctagaga tgtatacaaa caaataatta 136800 tttagccctt cctaatattc tggccgcctg agaacatatt gattcaaggc ggtcattact 136860 tatgtgatca tttctatacc agtacatagt tattgttcca gcatagatat tatccaaatt 136920 aaaatatgga caactataca tgtaatttat ttcgggagta ggctttttag ttggtaaaaa 136980 agcaaatttt gagttagaat aataatgacg tccatttaaa tgaactgcat attcatccat 137040 agttttatca acaggatatc ctccaagtga tttttcgctt attgttgaag gtaattttcc 137100 ttcatatgct atgatatcaa caaaatagtt taagttttta gggcggaaag aatacaccgc 137160 actaaagtct gcctcagatg atatatgaac tatctggagt tgttccaggg cgacagattc 137220 aaagcgtgca tttctttcct tttcaataat ttcactgtat gtttcatact ttgattgctt 137280 atagtactca aagaaactat ctcccctata ccaaacaatc gccatcataa ataaaagaat 137340 tacgacagct actcgggaag caagaacctt cccggtagcg ttatctttga acaagcgatc 137400 tagaacacca aacagaatac cagagggcga aaatgatatt ctaggtgctg ccatattacc 137460 tccaatttat caatatttat aactgagagg tattatcgtt taaatccagg agccgtagat 137520 cgtgcctgta gcgccccatg atggtgaaga accgaatact gccctgcctg gagcaccacc 137580 gccagatcta gataagtgat taccccatcc atctccgccc cgacctccag catcaccgcc 137640 attgcctccg ccgaattggg aagtgtaacc ggcaccgccc ggggcgtcat aactagcatt 137700 accaccaggc caattaacgc cgcctccgcc gatgccaaat ggtctaccac caccgccacc 137760 tgctgtagca tttggcgccc agctattctg taatgacact gcaccaccac caccgccgcc 137820 tgatgcaata acgccatagt tccaaatacg aagtcttccg ccaatatcat tttggattac 137880 atcgccgcca tttgtgcctg ggtttccgtt tacagtaccg ttaccacctc gtccccacat 137940 atgaactcca ccatgaatat tcaaagtaac atattcattt ggcgtatcgc catacataaa 138000 gaataatgga acatctttac tatatgatac caaatttccg gtgatattaa acactatagg 138060 tgcactacct gcctcaaaac atctatctcg gaaccattga ccattgaagt tatggtctgc 138120 tccaagcgtg tgaattactt caacagatcg acctatcata ttactcatcc aaaatggaac 138180 acccattctt aatcttacgg ctgctcctgc catccaaggg tctccagtct gtacccttgc 138240 agaccatcct acccacggtc cttcaactgc catataacct ccaaggggcc gaagcccctc 138300 ttattatttt aacaatgatt taactaattt tttaagctct tcaatctctg acttaagtcc 138360 tgcaatttct gcagtatgct cattgattgc agctgtattt aaaccaatta caccgttata 138420 gttcaaacga agtaaagctt caccattagg gtcaccttca accaattcag gtaaaatagc 138480 ttgaacttct tgggcaatta aaccagcgtt aggttcccat ttctgattgc cttcttcatc 138540 taggcctcgc ttctgcatat aagtgtaacc gttaatttta gaaagcttct cagaagcatt 138600 ttcaaattta acaaggtctt ttttaacgcg aatatcagaa cggacataaa catcacggac 138660 atatgtagaa tagttgtcat tcgcctgaac gagatgacca taagacataa tagctcttcc 138720 ttgctcagtc cacaaagcgc cagtggtatc ccaaagacct tcatggatat gataagagct 138780 agaccatgaa gcaatatgca tgccattggc aaccatattt attcggccgt caccacccca 138840 tactaagcca gtgtcattat caccaataac aacacatgcg cctgtccatg caggatcacc 138900 tccaccagct gatattctta ctccagcttt aaccatgtca ggaacgtaca tgtttccatt 138960 atggtggaat tcgaagatag cttgcggatc gctagcattt tcctggttac caacacgaat 139020 aatagcctga gcccattggt tagtgatacg gcgcatacca aaatctacgc ctgatacgta 139080 tccttcgtta gtaataccag attttccttt aataatagga taataggagt cattgctgac 139140 ggatccaaaa tctacaaata caggcgcttc taaaccccat tggtcacccc aggcgccaga 139200 tttatgcgca acccattgtg cagcctttat ataaagtcgg tccctattaa aataaaaagt 139260 atccgaccct tgaggagcta aaccaacttc tccgctatta tgaatactaa cgcctgaatt 139320 agtttggaaa ctataaaacg ataaggtgtt ctcagtgccg ccattaccaa cataccagtt 139380 attaacgcct gctttttgtc ctttcacata acaagattcg gttgcagcac tgttaataac 139440 aatagcatcc gagtttgcat tcagagttaa acggcctgtc atcgtgtcgc cttctttagc 139500 cacacgggag gcaatggcat cgcgaatatt agttacgtcg cctgctgaag taaacgtttt 139560 ccagatatca gaccagatat taccattgcg agcaatagta gcttcaccac caccgacaga 139620 aacgttatct gggaagttaa cagtaccgtc ttgattaaaa cgagatgtat ggccttgtga 139680 accatcttct ttgatatggt gtaaaacaaa tgtacccgaa ttaatttcag tacctaaaga 139740 ccaaacagct ttactgttta aaaacttctg tttaacaata ggataatatt tacttactgc 139800 gtcatcatcg atttcctgga aaataggagc tgcttgaaca tattgaatat cataaccacc 139860 agcgcccgcg ccgtgaccag taaatttaat ataaccgtta gcagcaaacg tattgtaact 139920 tgattgtaaa tctggaatag taactttacc agtatctaaa gccatactaa acgggcgaag 139980 tggaccgata tcaccatgtt taccttcacc ataagcagtt ggaataatat gaagactacc 140040 ttctgaacgt ctaaaaatag ctccataatc tgcgttccaa atacgaagcg cattcacagc 140100 cccagatgaa atttcgcctg ttgtgtgaac tcctttattt ccagcagtaa caccggatga 140160 ttcaaatgaa ccattgacgt tcatttctac ttcgccggta gtaagtcttt gaatatagct 140220 catccaacta gttgcgtcag aaatttccat gacattttta cgggtgcctt cactaggatt 140280 agtaaaggta ttaccccata cagaaattgt catattatca actgaactgt taccagttgt 140340 gttacctcta aacatcagcg tagaagaatt attacgacca tcaatattaa ctgaaccacc 140400 aataacgtca atattaccag gagtaactag caaaccgcca tgagtattaa tgtttgtggt 140460 acccttaccg cggaaataat gatgataatt accaccttgg taatatccta aaagagtctg 140520 accatctcca aacgcattat tatcatggta agtaacaaca gtagcgagag catagttttc 140580 agtaggagga gtagttaaat cattgccatt tacagaataa cccataacca tttttctaag 140640 gcttacggtt gattcaggac tataaaggaa agttctcgta ccattgttta ctacatagaa 140700 ataaccatct tgataccatt ttaatccggt atcattatca cccaaagcaa tagaaccttt 140760 acctaaggct gaatacgtca taggccctgc tgattccata cctactgcaa tcgaatgccc 140820 agcttgaaaa ccacctgaat aagcaaaaga caacatgaaa tcagtagggg ttgaaccaga 140880 ataccaactg atgccgttat tttcacaaat ttcatggagc attgcggccg ctgggttggt 140940 acgtacaacg cgcaaatagt ttttatctgt gtcagaatta ttattggcca ttgaatagat 141000 atcataatcg ccgacaactt taccatttgt aataacttta ttagtataaa ttacgggagt 141060 tgatacagat ttccacccaa aaacttctgg tgaagaaaac aaaccatttc cattaaaagc 141120 gaaagtgctt tctgaactag ctttgtaatc ttgaactctg atgttaacta cttgtgtagt 141180 taaaccgtca ttagcaggag aaaaaataat accgcgttca cggtcggcgg aatcatgaaa 141240 acgaacatgt gctgtacctg aactttttgt aataagttct ccgccttcag tcataatctg 141300 cccggcggca ataatatcgc gagttactcg agcaatacca gttaaattaa aattaccagt 141360 ctgagtgaag gttccattta aagtataatc accagtttga ttataatttc ctatatgaat 141420 aacattcccg tcaatgctac cgcctttagc aaaacccaga tcaatgatat ttccttggtc 141480 atctttagta aaaagtacgc ggtcttttaa atttatagcc aattcacctt cggctaatac 141540 tgaagcggca ggacgtgctc ctgcagtttt gcttctttta aattgtattt gttttaaagt 141600 agccataagt cctcttaata atagccgaaa tcttgaacag aatccttaat aacaatttga 141660 tcaaatcgtg gaacatgcga aggttgagat gcaggattct gtgaaaaaag gtttggcgca 141720 gttaaattac ctgtcatagt atctccagag cgtaatactc tagagtttgc gtttgctgta 141780 acaatattta ttgctccatc gacataatct ttgcgagtta agtgtgctgg gccggtcggt 141840 actgaagcat tagaaataac ttgttggcca gaaacaatat tgccaactgt ttcaacttta 141900 ccactcctgg cattaaacca gattgtgcgt ccctggatcc catcacttgt gcatgtgttc 141960 caaataccaa tgccgtacca tgaacgtaaa tccatgttag ctttttctct ggaagctcca 142020 tctccattgc cagaatataa cccattcagg tctgaaccgc cagaaaacgt tgcagaaatc 142080 ctaacgcctt ctttaaattc tacttctttt tggaaacctt gagactttgt gccaccatta 142140 gctttagaaa cgaaatcgtt atcagttgcc tgtggtttat tgtattctga atatatttta 142200 aatgatttat agagtacatc gtcaccggct ggattcaatg gaaaatttcc ttgatgccaa 142260 atgacagagc ctccagttgt tgaacctact tttaaatcag ccattgtatg cccctttatt 142320 ttaatagtat ttataaagaa aaagggaacc cgaaggctcc ctcaatttat acttctctaa 142380 attcttgccc aaatacttta ccagctttag acgatgcttg cgtaggaagt accattatat 142440 ccggaggtga agcagattca cagatataat taactcgaat accattgacg ccaaattcag 142500 caggttttga aattccgcca tttcttgaca cttccgaaaa gcttaaattc ctcatgccac 142560 cttgaccagc ttgtgcagtc ctttgcgcgt atatcgtaaa tcctactgca ttttctggaa 142620 taactacata atcttccttt aattcccatg acccagcttg tccagtaaac tcagcatgtg 142680 ttgaagaaat atatccattc gattcatcat aaaaacggat agatatattt gtagttccaa 142740 gcgcaagtaa atcggcatca gcatatagct gtgctttaag ataaagaaca tcgccaggaa 142800 ttaaattata atcagagagt ttacttatcg cggatgaagt tggtaaacgt gcaatttcgt 142860 tattagttcc accaaccgct gacataaatt gctcaacgct ttcgtatgtt cttttaggaa 142920 atcctgtagc tccaacgtct tctaaactat caaatacaac atctaaaata gtttgatagt 142980 catctgtgct ttttctatta cttagtttaa catgctctaa tgcaatagct cttttagaag 143040 aagtgtaaaa agcagcatat gatacgtcga atcttgacaa tacagaatca gatggaaaag 143100 cagacgttcc tgcggttctt atccaagata ctacttcagg aggaaaatta acttttccgt 143160 tagttaatat agcaacaagt ctattattcg tcaaagaatt catgaaacta acaaaagcag 143220 ctgatgtagt atcatttgaa gtcgaaaaag catatgactt actatcaact aatgctcctg 143280 tagaagggtc aaaaactctt aaatgaagtc ctgcgctaaa tgtttgactt ccgacaggat 143340 tatcctgaaa tttaacgtat ggtccttctg cagtagaaag cgggcaagaa cccgctatac 143400 ttattttata tcttactgaa ttgctttccg ataaaaatgg cgtttggaca tatccttgtc 143460 caaactctgc cataaatttt tccataatac ctcttattca atccattcaa atttaacaga 143520 tttattcact gggtcaggaa taatgcgaac attaccaatt cgtaagaaat cacgaatagt 143580 aagattcccc attgtagcat tatcagatgg taaagcaccg atatcagatg gctgaggagg 143640 gttaccccca tcaaatacct gaacaaaact tgaccaagag tttttggttt tctgccatgt 143700 acgcgtccag cgagtggtgc gcgcttctgg ggtcgttgga taagtaatcc aatcttggta 143760 aagcgaatcg agtgtgttac caaactgagt caatgtacca ggagatttaa cctcttcgcc 143820 acgttctaag tatggaagtc cagtcacttc attagttttt tcaaccattt caaaataacc 143880 cgggaactgg ttataagtgg ctgaatcatt aatatcgatt gaccagaatc ctacagtatc 143940 agatgttggc gcacgggtat ataaatcaga ggttttagta ccctgagaac gaattctcga 144000 gttaacagtt aaaccacctg aacttatagt agcacctttg gcaatgatta agctttcacc 144060 aatcgttact tgaccagatt gattattaat tgataatgga cgtaatccat taaatccacc 144120 gctctgatca ccggctgcag tgagcataaa ataggtgtca ttaccagcgt tacgaataaa 144180 gaatccataa ttaccattta ttgctctgaa agcattcgat gatttactga taaattcgcc 144240 attagcagta actgaactac cgaatgttgc aacgccattc gcattcaaag aacctaaagc 144300 attcacattt attggcatta cagtaccatt aatgctaaat gctatattac catctttatc 144360 gcgttgagaa taaaagtgat aagatgtttc atcactaact tcaaatacgg tagaacgtgt 144420 tgtgtttgat tccccactaa attgattccc ccacacgctg actgtcatcg tttgagccgg 144480 gttcgttcca gtttgaggtc ctttctcaaa aatcagacga gttgctgttc catcagtatt 144540 agaaatggtt agtgtactat ttgctgaaac tggtccaccg aacgtagcag tactagatga 144600 tacaagaggg gcactcagat tcgtttgttg cgttaaggtt agtgaaccat taactgtctg 144660 tgcaatatcc ctacgaatga actgagatga atctagacca tctaataaat tactatctgc 144720 agcttttgct tttaacggca aataatttgc taatacgcgg tttaattcat atggcgatac 144780 tgcatagcta tttttctcat ataattctaa tgactgtgtt gaaccaacta tatcattacc 144840 aacgaatgta attgaaccag atgaagtttt aacaaaacct cttaccaaag tagtagctgc 144900 ccaagtaggt tcactctgta caacccattt taaatttttt ggagatacag cagtatttgc 144960 tgacgttcca gctacagttt cagcctgagt tgcaacttta ataacgcctt cttgcgattc 145020 ggtagattta gtacctaaaa gctttttagg agttattaga acattatcca atgttcctgc 145080 agcagcttca acttgtgtag ctacacgaag tgtaccacgt tgtgtctcat ttgcttcaag 145140 aatattaagg gtataatggt cccagagagt tcctgattca actaatccag atagagcaac 145200 aacagaagta cgatcagtac tattaaatct ggttttaatt tttaatggtg tagagatacg 145260 agtatcgtcg acgcctgcgt cgaattcaac ttgcgtagca atttcagcta taccacttaa 145320 accttcagtt gctttacgat catttaaagt tttaggagtg actgcacgag tataatcagt 145380 tcctgtatta acttcacttt gcgtagcaat ttcaattaaa ccaattcttc catcagttga 145440 tgtcttttta tgtaacgttt ctggcgttac aaccgcattt gcccatcctt cttgactctg 145500 tccagcaatt acttcacttt caactgctaa aattactgca ccttgttgtg ttggagtagc 145560 tttatactga tctaaagctt taggcgaaac aactaaatta ttagtgtttt tattgtaaac 145620 atttgtacca tttaattcac ggctagaagc tggagtagct cctgcggtag atacaaaagt 145680 tacgatacca gataatgatt cagaaccttg acgagcttga agctttttag gagtgatgat 145740 tgtagtatca tcggttcctg tattagtttc ttgctgcgta gcaatttctg cgacacctct 145800 gcgagtttca gtagcagttc tttcattcag ctttttagga gtgatgataa tatcatcagc 145860 aaaagagaat gtagtgtcct ggttcacttg ggcagtagtt gctattcttg cgatacctct 145920 acgagtttct gtagcagtac ggttagctaa tgtttctggt gtaattgcta attctttctg 145980 tggagaattt tctaaatcag catttgcttg agcttgtgta gctaaagcaa ttacgcctaa 146040 tcttgctcta gtagaatcat ttaaagaatc aactctttca actgttggaa cgttttgttg 146100 tacaacccag tattttccgt cagaatcttc gatataagca agttgtaaaa ctggaacata 146160 attagtttca ccgttaaaaa ctaattcttg aacagtcacc cattcagctt caggcggata 146220 ctctgaacgt tttgggaatt gcagcaattg aactgaagaa gcaattttat cttcaccggc 146280 ggctttgatt ttaactgttt gtccttttct catgtaattc atggaaattt taacagtatc 146340 accaacagaa atattagttg gaagctgaag ttcaattgtt tgagttgttc cgttattcgc 146400 accaaatacc ataacttctt catttggacg aatactagaa ttagttgtta taatacgtaa 146460 acgtgcttta ctatccccgt caaacaatct ccacaatttt tcattatcat catacatcaa 146520 gaaaccgtca atcgatgtac gtccttcaat agaatgagtt ccatcttctt gtattgaagt 146580 agtttcatcg tatgtagtaa caattgtatg ataaagtgga tttagtttat ctaaatcgac 146640 gaaattaata atatcgccat gattagcaaa tctcggaagt ttaatattaa tcggcgcagc 146700 ggaagtaaat ctacgcacga taaaatcgtt agattgcgcc tgataaagac ttgccggtgt 146760 tacaattgca gcttctctgc tataatcagc aacgtacatt tgccacagac gattactaaa 146820 aattaaaact atctgtgatt ttggatgagt cattagtact gatcgtactt gctgacctct 146880 aaaatttaca atgctttgca ccggagctgt aattaaaact tggttaactc caggttttcc 146940 tccaatgtct tggagaacga tagtatctcc atcaattggg gaagatggta aagtaaatgt 147000 aatgtcatta ccagctgcag tatcaactga aattgattcg cctgacttta attgataagg 147060 tcctgatgaa acagttgtcc aattagcatc agtacgtaat gatctccaac gtacgctatt 147120 aaaagctcct gctggttttg gaatatcatt tatagcagcc caaaagcggt tatcataaat 147180 gattacaaaa tcttttaaat atccacgagt tggatcatat tgttgaactg tgttttcttg 147240 aattaagtaa tcaacgttga caccgtcagt tcctacggta cgatcagcta aagctacgtt 147300 gattatttta tcgccacctg cgtccagacc gtcttctgct ctgaactttc ttttaatctc 147360 ggccattctc ccgggctcct aatgtgtttt caataataag tatttatact tgtttacttt 147420 aagatttgga tagtatataa tagaaatctc actaattgaa cgaggttcat atggatttag 147480 aaatgatgct ggatgaagat tacaaagaag gaatttgctt aattgacttt agtcaaattg 147540 cgctttcaac tgctttggta aactttccag ataaagaaaa aattaattta tcaatggttc 147600 gtcatttgat attgaactca attaagttta atgtcaaaaa agcaaaaact cttggataca 147660 ctaaaattgt actgtgtatt gataacgcga aatctggata ctggcgtcgt gattttgctt 147720 attattataa gaaaaaccgt ggaaaagcac gagaagaatc tacttgggat tgggaaggtt 147780 attttgaatc cagtcataaa gttatagatg aactgaaagc ttatatgcca tacatcgtta 147840 tggatattga taagtatgaa gcagatgacc atattgctat tcttgttaaa aagttctctt 147900 tagaaggaca taagatttta atcatttcat cggatggtga ctttactcaa cttcataaat 147960 acccaaatgt taagcaatgg tcaccgatgc acaagaaatg ggttaaaatt aaaagcggtt 148020 ctgctgaaat tgactgtatg actaaaatcc ttaaaggtga caaaaaggat aacgttgctt 148080 cagttaaagt acgatctgac ttttggttta ctagagttga aggtgaacga actccttcaa 148140 tgaaaacctc tattgttgaa gctattgcta atgaccgtga gcaagctaag gtgcttctta 148200 ccgaatctga atataatcgt tataaagaaa atttagttct aattgatttt gattatattc 148260 ctgataatat tgcttcaaac attgtgaatt actataattc atataaatta ccaccgcgtg 148320 gcaaaattta ttcatatttt gtaaaagcgg gtctttctaa attaactaat agcattaatg 148380 aattttgagg tgaataatgg ctaaaaaaga aatggttgaa tttgatgaag ctatccatgg 148440 cgaagatttg gctaaattta ttaaagaagc atctgatcat aaactgaaaa tttctggtta 148500 taatgaattg attaaagata ttcgaattcg tgccaaagat gaacttggcg ttgatggtaa 148560 gatgtttaat cgtctgttag ctttgtatca taaagataac cgtgatgtgt ttgaagctga 148620 aactgaagag gtagttgaac tttatgacac agttttctct aaatgatatt cgtccggtcg 148680 atgagaccgg tctttcagaa aaagaacttt caattaagaa agaaaaagat gaaattgcaa 148740 agcttcttga ccgtcaagaa aatggattta ttattgaaaa aatggtagaa gagtttggaa 148800 tgagttatct tgaagctaca acagcattct tggaagaaaa ttctattcct gaaactcaat 148860 ttgctaaatt tattccttcg ggtataattg aaaaaattca gtcagaagct attgacgaaa 148920 atcttttacg tccttctgtt gttcgttgtg aaaaaactaa tacattagat tttctactat 148980 gattaaactc cgcatgcctg ctggtggtga aagatatatt gatggtaaat cagtttataa 149040 attatactta atgataaaac aacatatgaa tggaaagtat gatgtaatta agtataattg 149100 gtgcatgcgg gtgtctgatg ccgcttatca aaagcgaagg gataagtatt ttttccagaa 149160 gttatcagaa aaatataaat taaaggaact tgctttaatc tttataagca atcttgttgc 149220 taaccaggat gcttggattg gtgacatctc tgacgctgat gcacttgtgt tttatcgtga 149280 atatatcgga cgcttaaagc aaattaaatt taagtttgaa gaagatattc gcaatattta 149340 ctatttcagt aaaaaagttg aagtttctgc ttttaaagaa atttttgaat ataatccaaa 149400 agttcaatca agttatattt ttaaactact tcaatcgaat ataatttcgt ttgagacgtt 149460 tatcttgctt gattcgtttt taaatataat cgataaacat gatgaacaga ctgataattt 149520 agtctggaat aattattcta taaagttaaa ggcttataga aaaattttaa atattgattc 149580 acagaaagct aaaagtgttt tcattgaaac tgtgaaatct tgtaagtatt gatatgaata 149640 tagtatattg gtttacattt gaagaccgtg tcaaaaataa gactccgccg tactactata 149700 tcggcagtaa attaaattgc tcatttgaga atggaataat atatgactct tccggaaagg 149760 aatactggag ttcgtgtaaa caaaaaagat ttttaaatgc ggtaatgctt caaaaaccga 149820 gcgttaaaat aattcaaatt gatgatgatt tggatgttat tgaagcagag cgaaaatacc 149880 aacttgaagt aaatgccaga gataatccag actattttaa tctggtatat gctggcggcg 149940 ggtttggtgt gagtggtgaa actcatccag ccaaagaccc ggaagttaga gagcatatga 150000 gattggctaa ttatatgaac cgtgacgatt ttagaccttg gaaaacatca cgagctaata 150060 tagagtcttg gaaattatct catattgctt acgagaatta tgtgttatta ttatcctcta 150120 atctgtacgg taaaactcct ggatggcgaa gagttaaagg taatataaat ataactgatg 150180 caactgctaa atcgatggta aagtatttca actcaggttg gatacctctc gaagacccag 150240 agtattgcga attatgccag ctatgaggta aagtgtcata gcaccaactg ttaattaaat 150300 taaaaaggaa ataaaaatgt ttaaacgtaa atctactgct gaactcgctg cacaaatggc 150360 taaactggct ggaaataaag gtggtttttc ttctgaagat aaaggcgagt ggaaactgaa 150420 actcgacaat gcgggtaacg gtcaagcagt aattcgtttt cttccgtcta aaaatgatga 150480 acaagcacca ttcgcacttc ttgtaaatca cggtttcaag aaaaatggta aatggtatat 150540 cgaaacatgc tcatctacct acggtgatta cgattcttgt ccagtatgtc agtacatcag 150600 taaaaatgat ttgtataaca ctgacaataa agagtacggt cttgttaaac gtaaaacttc 150660 ttactgggct aacattcttg ttgtaaaaga cccagctgct ccagaaaatg aaggtaaagt 150720 atttaaatac cgtttcggta agaaaatctg ggataaaatc aatgcaatga ttgcagttga 150780 tgttgaaatg ggtgaaactc cggttgatgt aacttgtccg tgggaaggtg ctaactttgt 150840 actgaaagtt aaacaagttt ctggttttag taactacgat gaatctaaat tcctgaatca 150900 atctgcgatt ccaaacattg acgatgaatc tttccagaaa gaactgttcg aacaaatggt 150960 tgacctttct gaaatgactt ctaaagataa attcaaatcg tttgaagaac tgagcactaa 151020 gttcagtcaa gttatgggaa ctgctgctat gggtggtgcc gcagcaactg ccgctaagaa 151080 agctgataaa gttgctgatg atttggatgc attcaatgtt gatgacttca aaacaaaaac 151140 tgaagatgat tttatgagct caagctctgg cagttcatct agtgctgatg acacggacct 151200 ggatgacctt ttgaatgacc tttaacagat tatattacta attaattggg gaccctagag 151260 gtcccctttt tttatttcaa aaattttttc acaaagctgt ttacatcctt gtccttccat 151320 ggtactatac aactatcggc aatactgctg atgattaaag aggaaaataa tatggctaaa 151380 gttgatattg acatcgttga ttttgaatat attgaagaga ttattcgtaa tcgttatcct 151440 gaacttagta tcacaagcgt gcaagattct aagttttgga gtattcaaat cgttattaaa 151500 ggtcctcttg aagacctcac cctctttatg gctaatgaat attgtgatga catgggttct 151560 gaagacgcag aattttacat gggattgatt gaacaataat tatcaagggg ctatcaagcc 151620 cctattaaaa tgaggaaaat caaaaatgga aatcggcaaa aaatatgagt taaatccaca 151680 ccgtattaaa tctttcattg atattagttc atcaaatgct agtatggtcg gcatcattca 151740 agaaaatggc ggttggtttg aagttaaatc aatatcaagt ttagatggat ttgattatgt 151800 aaccgaaatc atttgcgcca atggtgaaat ctataatgat gatggtatgg gtgatgatta 151860 ttttgaactt agtgaagaag agttttattg ttttcgcgag tataaagaac caacttttga 151920 agaagatgaa gtcgaagaca ggatttctgg cgtaacaaaa attcactgca ttgtagatga 151980 aaacaatgta gatgaaatca ttgaactttt gcgaaaaact ttcaaaaagt agtttacaga 152040 agggtagtag cgtgatacta ttaccctatc aattaaggag aataaaatga gattacaacg 152100 ccagagcatc aaagattcag aagttagagg taaatggtat tttaatatca tcggtaaaga 152160 ttctgaactt gttgaaaaag ctgaacatct tttacgtgat atgggatggg aagatgaatg 152220 cgatggatgt cctctttatg aagacggaga aagcgcagga ttctggattt accattctga 152280 cgtcgagcag tttaaagctg attggaaaat tgtgaaaaag tctgtttaag gaaaataata 152340 tgatttttgt atttgaattt atgaatgatg aattcgatta tgcaattttt aacgcattgc 152400 ataatcctga tttaagtgaa tttaatgaaa tgttttctga cgctttgagt atgtcagaag 152460 aatactgtgg agaatgtcaa cgtgtttgtg tgacagtttt tgaaaacaaa gaaaagacat 152520 atgaagaatt attctttgac gctaataaag ccactgaatg gtttattgaa aggggctttg 152580 cgtaatgatt aaattggtat tcgcttattc tccaactaaa accgttgaag gctttaatga 152640 attagcattc ggtttaggtg atggtttacc atggggacga gttaaaaagg acctccagaa 152700 ttttaaagct cgtactgaag gtacaattat gattatgggt gctaaaacgt tccagtcatt 152760 atctacatta cttcctggac gtagccatat tgtggtgtgt gaccttgcgc gtgattatcc 152820 tgtaactaaa gacggcgatt tagcacattt ctatattact tgggaacagt atataactta 152880 catttctggc ggtgaaattc aagtgtcaag ccctaatgca ccattcgagg ctatgcttga 152940 ccagaattct aaagtaagtg taattggcgg acccgctctg ttatatgctg cattacctta 153000 tgcagatgaa gtagttgttt ctcgtattgt taaaaggcat cgtgttaatt caactgttca 153060 attagatgca agttttcttg atgatataag caagcgtgaa atggttgaaa cgcattggta 153120 taaaatagat gaagtaacaa cccttacgga atcagtatat aaatgaaata acgcgtggca 153180 gaaaatatga attttaatta ttaccctatt ttattagaaa aagacgcgaa acagccaaag 153240 tggcagggtc ctcagtttat taaaggtgtc tatcaattag tagttcctaa agacaagatt 153300 tatagcagtt gtttcactga atccgcttgc agtattttcg gtaatagttc tccgtattgg 153360 aattttgaca taaagctgga tagaaatatc gatatttggt tgaaagctat ggatattggt 153420 aatatcacgt ttgatgagaa taattatcat attattggtc gcttttccaa acgcggtaaa 153480 gaattatatt tcacgcctga aattgagaga aaatttgatg ctaaaccgta ttgattattt 153540 agcttcataa aatgtattat aatttatata aggtactaaa tgaaacaata ccaagattta 153600 attaaagaca tttttgaaaa tggctatgaa accgatgacc gaacaggcac aggaacaatt 153660 gctttgttcg gtactaaatt gcgctgggat ttaaccaaag gttttcctgc agtaacaact 153720 aaaaagctcg cctggaatgc atgcattgct gagctaatat ggtttttatc aggaagcaca 153780 aatgtcaatg atttgcgatt aattcaacat gattcattaa ttcaaggcaa aacagtctgg 153840 gatgaaaatt acgaaaatca agcaaaagat ttaggatacc atagcggtga acttggtcca 153900 atttatggaa aacagtggcg tgattttggc ggtgtagacc aaattataga agttattgat 153960 cgtattaaaa aactgccaaa tgataggcgt caaattgttt ctgcatggaa tccagctgaa 154020 ctcaagcaga tggcattacc gccttgtcat atgttctatc aatttaatgt gcgtaatggc 154080 tatttggatt tacagtggta tcaaagaagt gttgacgtgt tcttgggctt gccattcaac 154140 attgcatcat atgctgcgtt agttcatatt gtagctaaga tgtgtaatct tattccggga 154200 gatttgatat tttccggtgg taatactcat atctatatga atcacgtaga acaatgtaaa 154260 gaaattttac gtcgtgaacc tatggattta tgcgaattgc aattaaaatt tccagatgaa 154320 tttgatgaat gggacacaga atcgcaggta ttttggttga gtcaattcgc aaagccgcat 154380 aattttgttc ttaacaacta tgaatcacac cctcctatta aaggaaagat ggcggtgtaa 154440 ttttaattta attgcgagga tatatgattt tacgatttaa agatacttct ggtgtagttc 154500 tttttacact tcctaatcca agtgagttag aagttccagg accaaatcag cctattatca 154560 tttatggcaa aaaatattat actcataaaa tgactcgtga gtattttgat aataaaattt 154620 ctacagttaa aacttcttct gactgttact acgatattac tgttttaacg gaagaacaat 154680 atgaacacct acaaccgcgc gggccgtcta tgccgggtag tgaataaata taaatccgac 154740 tttgatgtta atattcaccg tggtacattt tgggggaatt acgtcggtaa agatgctggc 154800 agccgggagg ctgccattga attattcaaa aaagatttta tacgtcgaat taaatccgga 154860 gaaataacta aagcacattt agagccttta cgtggaatga ggctaggatg cacatgtaaa 154920 ccaaagccgt gtcatggtga tataatagct catatagtta accgattgtt taaagacgat 154980 tttcaagttg aggacttatg caattaatta atgttatcaa aagtagtggt gtttctcaga 155040 gctttgaccc acaaaaaatt attaaagttt tatcttgggc agctgaagga acatcagtag 155100 atccttatga attatatgaa aatattaaat cttatctccg tgatggaatg acaactgatg 155160 atattcagac tattgtcatt aaggctgctg cgaattctat ttcggttgaa gagcctgatt 155220 atcaatatgt agctgcacgc tgtttaatgt ttgctcttcg taagcatgtt tatgggcagt 155280 atgaaccacg ttcatttatt gaccatattt cttattgtgt aaatgaaggt aaatatgacc 155340 ctggattatt gtcaaaatat tcagcagaag aaattacatt tttagaatca aaaattaagc 155400 acgaacggga tatggaattt acttattccg gggcgatgca attaaaagaa aaatatctag 155460 ttaaagataa aaccactggt caaatttatg aaactccaca gtttgcattt atgactattg 155520 gaatggcact gcatcaagat gaacctgttg acagattaaa acatgttatt cgtttttatg 155580 aagcagtatc tactcgacag atttcattgc caactcctat tatggctggt tgtcgtactc 155640 caactcggca gtttagttca tgcgttgtta ttgaggcagg tgattcgctg aagtctatca 155700 ataaggcttc cgcttcaatt gttgagtata tttctaaacg cgctggaatt ggtattaacg 155760 ttggtatgat tcgtgccgaa ggttctaaga ttggcacagg tgaagtgcgt catactggtg 155820 ttattccttt ttggaaacat tttcagactg ctgttaaatc atgctcacag ggtggaattc 155880 gtggcggcgc tgctactgct tattatccta tttggcattt ggaagttgaa aatcttctcg 155940 ttttgaaaaa taacaaaggt gtagaagaaa accgtatccg tcatatggat tatggcgttc 156000 aactgaatga tttgatgatg gaacgttttg gaaagaacga ctacattact ttgttcagtc 156060 cacacgaaat gggtggcgag ctttattatt cttattttaa agaccaagac cgtttccgtg 156120 aattgtatga agccgcagaa aaagacccta atattcgcaa aaagcgtatt aaagcacgtg 156180 aactgtttga attgctcatg actgaacgat caggaacagc aaggatttat gtacagttca 156240 ttgataatac gaataactat actccattta ttcgtgaaaa ggcacctatt cgtcagagta 156300 acttgtgctg tgaaattgct attccaacaa atgatgtgaa tagtcctgat gctgaaattg 156360 gattgtgcac gttatctgca tttgtgttgg ataacttcga ttggcaagac caagataaaa 156420 ttaatgaatt ggcagaagtc caagttcgtg ctcttgataa tcttttggat taccaaggat 156480 atccagttcc tgaagcagaa aaagctaaaa agcgtcgtaa ccttggtgta ggtgttacta 156540 actatgcagc ttggctggca agtaactttg cttcttatga agatgctaac gatttaacac 156600 atgaactatt tgagagatta cagtatggac tcattaaagc atccattaag ctcgccaaag 156660 aaaaaggacc ttgcgaatat tattcagaca ctcgttggtc tcgaggcgaa ttacctatcg 156720 actggtacaa taaaaagatt gaccaaatcg cagctccaaa atacgtttgt gactggtcgt 156780 cgctgcggga agaccttaag ctctttggca tccgtaatag cacattatca gcacttatgc 156840 catgtgagtc atcttcccaa gtttctaaca gtacaaatgg tatcgagcct ccacgtggac 156900 cagtctctgt taaagaatca aaagagggtt cctttaatca agtcgtgccc aatattgagc 156960 ataacataga cctatatgat tatacgtgga aattagctaa gaaaggtaat aaaccttatc 157020 ttacgcaagt agctattatg cttaaatggg tatgtcaatc agcttcagct aatacatatt 157080 atgacccgca gatttttcca aaaggaaagg ttccaatgtc aataatgatt gatgaccttt 157140 tgtatttttg gtattttggc ggaaaaaatt tctattatca taatacccgt gacggttctg 157200 gtactgatga ttatgaaata gaaactccaa aagctgaaga ttgttcatcc tgtaaattat 157260 gatataattt gactcacgga cgagtcacta tctattaact aagcggaaaa tttatgagca 157320 cagtttttaa tacaaatcca gttgatgttt taaaagaacc tatgttcttc ggttcaggtc 157380 ttggtattgc gcgttatgat attcaacgtc ataaagtttt tgaagattta accgaaaagc 157440 aattatcatt tttctggcgt cctgaagaag taaacttgat gatggatgct gcacaattta 157500 acaagcttcc tcaatatcag caagatattt ttactaataa cctgaagtat caatcacttc 157560 tagatagcat tcagggtcgt gcaccatctg ctgtacttat gtcgttaatt tcagacccaa 157620 gccttgatac atgggttgct acatggactt ttagtgaaac tattcacagt cgttcataca 157680 ctcatatcat gcgaaatctt tatactgatc catcaaaggt atttgatgag attgtattag 157740 ataaagctat tatgaaacgt gccgagtcca ttggtcgcta ttacgatgat gttctagtta 157800 agactcgtga atatcagaat gcggttgaag attatgaagg aattttatca gaaggatttc 157860 ataaagaaga gctaggcaaa gttgttgaat atactaaacg ggcactaatg aaatctcttt 157920 atctttgcct tcatgtaatc aacgcattgg aagctattcg tttttatgta tctttcgcat 157980 gtacctttaa cttccataaa aacatggaaa tcatggaagg taatgccaag attatgaagt 158040 tcattgctcg tgatgaacag cttcacctta aaggtaccca atatattatt cgtcaacttc 158100 aacttggcac tgatggtgat gaatgggtta aaatcgccca agagtgtgaa caagaagcag 158160 ttgatatttt tatggaagtc aaccgccaag aaaaagactg ggcagttcat ttatttaaag 158220 atggcgatgt tcctggatta aatacaaata gcatgtggag ctttattgat tacttaactg 158280 tatctcgtat gaaacagtgc ggtcttccat gcccaattac cgatgctccg gttaaacacc 158340 cgtatccttg gattcgtgaa tatcttaatt ctgataatgt tcaatccgcg ccacaagaag 158400 tagaactgtc atcttatctt gtagcacaga ttgataatga tgttgatgat aaagttatga 158460 tgagttttaa aaaatatttt taaggagtgg gccgcaaggc ccattttatt atgaaagaaa 158520 ttgcaacaga atattcattt attaaatata ctgagctaga attagacgac aacggaagta 158580 taaaacaatt atccattcca aacaagtata acgtaattta tgctattgct ataaatgatg 158640 agcttgttta tattggaaaa actaaaaatt tacgtaaaag aataaattat tatagaactg 158700 ctattaatcg taaagacaaa acgtctgatt ctactaaatc tgcattaatt catgctgcgc 158760 taaaggaagg aagcaaagtt gaattttacg cccgccaatg ttttaatctt tctatgacaa 158820 atgagttagg tacaatgaca atcgcaacga ttgacctaga ggagccgcta ttcattaaac 158880 tgtttaaccc gccttggaat attcaacaca agaaaaaatg atgcttccac atggagtgtg 158940 gtactatatt caaaacacaa aagaggatac acaatgcaag aactttttaa caatttaatg 159000 gaactatgta aggattcaca gcgtaagttt ttttactcag atgatgtaag tgcatctgga 159060 agaacttaca gaattttctc atataattat gcatcttatt ctgattggtt acttccagac 159120 gcattagaat gtcgtggaat tatgtttgaa atggatggag aaaaaccggt aagaatcgct 159180 tctcgtccta tggaaaagtt ttttaacttg aatgaaaatc cattcacgat gaatatcgat 159240 ttaaatgacg tcgattacat tctaacaaaa gaagacgggt ctttggtatc aacttattta 159300 gacggtgatg aaattctgtt caaatcaaag ggttcaatca aatctgagca ggctttaatg 159360 gctaatggaa ttttgatgaa tattaatcat caccagttgc gcgacagact taaaaaatta 159420 gccgaagatg gatttactgc taactttgaa ttcgttgctc cgacgaatag aatcgttctt 159480 gcttatcaag agatgaaaat cattttattg aatattcgtg aaaatgaaac aggcgaatac 159540 atttcatatg atgatattta taaagatgct attcttcgtc catatctagt tgaacgatac 159600 gaaatcgata gtcctaaatg ggtagaagaa gctaaaaatg cagaaaacat cgaaggctat 159660 gttgccgtga tgaaagatgg ttctcatttt aaaattaagt ctgactggta cgtgtctctt 159720 cacagcacaa aaagttcatt agataatcca gaaaaattgt ttaagactat tattgatggt 159780 gcatcagatg atcttaaagc aatgtacgct gacgatgaat attcatatag aaaaattgaa 159840 gcatttgaaa cgacttatct gaagtactta gaccgagctc tgtttttagt tcttgactgt 159900 cacaataagc attgcggcaa agatagaaag acttatgcaa tggaagcgca aggcgttgct 159960 aaaggtgctg gaatggaaca tctgttcggt atcatcatga gcctctacca agggtatgat 160020 agtcaagaga aggtcatgtg tgaaatcgaa cagaattttt tgaaaaatta taaaaaattt 160080 atcccagaag gatactaagc tgtttacaag tccctcgtgt tgtgttatag tagtcttact 160140 gacataacat gaggacttta tgatggattt gcagcttatt actactgaga tggtcgttga 160200 agcatacggt gatactacag atgggatttc tgtatttaaa ggaaatcgtc gagttggata 160260 tatcaccgat cttaagaaag atttagctaa gcaagtcaag cggaaaacga ccattaaaga 160320 atatcgaaat cgtcgtcttg agcaagcccg tgatatgctt cctgatgcgg ttgaggagat 160380 gaaagtcttt ttagaaaatc agcttgcgaa atatgattgt gatgtgttca ttaatcagac 160440 tcaacctaat gttcatatta acaactgtaa atgctatatc atcgttaatc ctttaacggg 160500 aaaacatcgt cttggaatta gtaatccaaa tcgtagtgca tcggatatgg cagaagatgt 160560 tgaggcatgc tttaaaattt ctaaatctcc ggctgaacat catattttaa ttaacggtct 160620 ttctcaagac gatattatag aggttattaa aactttatgc aattaagtaa tacgacaaca 160680 ggcttgctat taattgtaat tgcattgggc ggaacttctt taattttaaa aaataaaatt 160740 gaaagattag aaacgtctgt tgtagaaatt acaaaaacgg ccaatgaaaa cgctttagca 160800 ttaaataatt tgcgaattca gtataattat attgatgcga tgaataataa aaatcgtgag 160860 gcaattgctg ctattgagcg tgaaaatgaa aaactgcgca aagacgcaaa gaaggcggat 160920 gtggtggctc ataagccagg attggttgaa aaacaaatca acaactcctt caacaagttc 160980 gcagaagaca tccaggacct ttctaaatga ttaaactatc agcagtaata ttatctattg 161040 gtcttctagt tggttgttcg acaaagcctc tagaagtaaa gaaagaaaca gttcatccta 161100 attggccagt acaaataaag tcatacgatg aagctaaact atcctggcaa gttaaagtta 161160 ttgatggtaa agcttgggtc ggtatgccat tcgaagattc ccaggaattt cgtatttggc 161220 ttaatgatgt aaaacgatat gtacatgatc agaaaactat gatatgttat tatcgccaag 161280 agttaaaaga ggataaatgc aaatgatttc atggcatcaa tttgaacatc tcaaaggatt 161340 gatttatgaa tccgagatgg ctgcaatgat ttacggacgc cagattcagc ggttagaatc 161400 tttacctcca actaatgatg ttttattagc tcaatctcgt gctaatctta aaaatgaata 161460 ccaaaataag tggggtaaag catctaaaga cctgcatgat tatattcaat cattggttga 161520 gaaataataa atgaaaactc tgttagaacg ttatattgaa tgttcagacc gttacattga 161580 tgcatgccgc ggtgcagtat atatggattt ggaccgcggg gtagtattaa atgatgaaga 161640 ccctgcgaaa gctttagatg atgcaggtaa agcattacga aaagaagcaa aagcccgtgg 161700 gcttgatatg tatcagctta aaaatcacat gataaaattt atttcatcca atgttcagag 161760 caaatcggtg aatcaatcaa cagctgaatt gtataaagga cggcgtgagc ataatattcg 161820 tattcttgaa gttttcttag gaattaaatg atgaaaaaga ttattttgac tgttggatgt 161880 cctggttcag gtaagagcac ttgggctcgt gaatttattg ctaaaaatcc tggattttat 161940 aatatcaatc gtgatgatta tcgtcaatcc atcatgggcc atgaagaacg cgacgagtat 162000 aagtatacca aaaagaaaga aggtatcgta actggtatgc agtttgatac agctaaaagt 162060 attctgtacg gtggcgattc tgttaaggga gtaatcattt cagatactaa cctgaatccc 162120 gaacgacgac tagcatggga aacttttgcc aaagaatacg gctggaaagt tgaatataaa 162180 gtgtttgatg ttccttggac tgaattggtt aaacgtaact caaaacgtgg aactaaagca 162240 gtaccaattg atattttacg ttcaatgtat aaaagcatgc gagagtatct cggtcttccg 162300 gtatataaag ggactcctgg taaaccaaaa gcagttattt ttgatgttga tggcacatta 162360 gcaaaaatga atggtcgcgg tccttatgac cttgaaaaat gtgatactga tgttatcaat 162420 cctatgattg ttgaactagc taagatgtac gataagcaag gatattacat tgtagtcgtt 162480 tcaggccgtg aaagtggaac cgaagaagac ccaacgaaat attatcgtat gacccgtaaa 162540 tgggttgagg atattgctgg cgttccatta gttatgcaat gtcaacgcga acaaggtgat 162600 acccgtaaag atgatgtagt taaagaagaa attttctgga aacacatcgc accgcatttt 162660 gatgtgaaat tagctattga tgaccgtaac caagtcgttg agatgtggcg tcgtatcggt 162720 gttgaatgct ggcaagttgc ttatggagat ttttaatggc gtggcatcat gaaacttggt 162780 ctattgttat tgtaaatagt ggtttagttg gtactagtaa tgggcaattt tgtgtattca 162840 ctagtgaaat cagagcttgg gaggaatgtc ttaaattaag agaaaagaat cctgatgttg 162900 aactaacagt aaagaaaact aaactgcctt taccatggaa aacttatgaa taacatagaa 162960 aagatttatc aactttgcga taaaattgaa aaagaaaaga aatatctatt ttgtttatgg 163020 cctattgttg atggaagaat agacttagat attcttgatt atgaaacaga agacatagta 163080 gatggttcaa cttttgataa tgcgttggat gttattaatt ggcttgaaga aaattatgtg 163140 gggtgaatat gtttccgact tattctaaaa tcgtagaagt agtgtttagt caaattatcg 163200 ctaataatat gtttgaaaag cttgataacg cagccgagct tcgaattcat gctcaagtga 163260 ctcatgtatt gaacactttg cttccagacc aggtggattc tattgcaatt acgctgtatc 163320 cgggttccgc gcatatcatt gtcgtatttg gtcttgatgc tgagctagtt atcaaaggcg 163380 atattcgttt tgaatcgcag acatcagaat tcaaagcaat ttaatagttt actttacggt 163440 agagttgtga tattatagct ctaccaaaac aaatgaggaa attgaaatga gcgaatggtt 163500 tgaagaagat aaggtttatc gctttaaagc tggatataaa gatattttta atgaaacttg 163560 tggggctaat aaacgaattg ctcagtttat tggggaaaat tcatttaaag taaaaataga 163620 tcctgcgaaa aatgttatta gcattaaacg cgaaattgac gattgctggt ataaagctgt 163680 tgatgtaatg ggcgaatcct ataaagttag cccgttattt tcaattgctt atatgttaga 163740 atattctttt ttcgaagaag ttcaaaaaga tgattctgtc agtaaatttg aaattaaaac 163800 tgataaagaa attaagtgga aagtagtagg tattactggt tgcatgtttt atatctatgc 163860 tcaaactgat acgaaggaag aagctaaaaa gaaagctcta gaatatcttg aagagtataa 163920 agaaggcccg gtaatgatta cccaagatgc cgaattagtt tctgtcaaat tagttaaaaa 163980 cgttgaaagt aaggagctgg gatcaacatg ctaagtgaaa aaccaattac tgttaaagaa 164040 ttccaagaaa aagttaaact atttgctcag gaattggtaa ataaggtttc tgaacgattt 164100 cctgaaacat cggttcgtgt tattaccgaa actcctcgtt cagtattagt aattgtgaat 164160 ccaggtgatg gtgatcaaat aacacatctt aaactggatt ttgatggatt agttgaagca 164220 caaagggtgt atggcgtact atgatgaatt taactgatat aattgataat tgtcttgaaa 164280 atgatactgg cgatcataga gcgcttgatt ctgaaacagc acagttcatt agaataactt 164340 taatgaatga tactctagtg aatagtattc atccttctgt gtatgatgct attattgtga 164400 cgaagtatcc agttgagctt cacaaaaaga tggttggtgc aatttttatt gataagaaaa 164460 accgctttaa agatgggcag aatataatta gttctgttat taaaagtata actaaacttc 164520 gtcacgaaat ttatcgtgtt gaaactgcta aatctgctta tctggtgatt atgaaatgaa 164580 agcgagtaca gtacttcaaa ttgcatattt agtatcacaa gaatcaaaat gttgctcctg 164640 gaaggtagga gcagtaattg aaaagaatgg acgtattatt tctactggat ataatggttc 164700 acctgcaggt ggtgtgaact gctgtgatta tgctgctgag caaggttggt tgctgaataa 164760 gcctaaacat actatcattc aaggtcataa gcctgaatgc gtatcgtttg gttcaactga 164820 tcgttttgtc ttggcgaaag aacaccgtag cgctcactct gaatggtcgt ctaaaaatga 164880 aattcatgct gaactaaatg cgattttgtt tgctgcacga aacggttctt caattgaagg 164940 tgctactatg tatgtaacac tttctccttg tccggattgt gcaaaagcga tagctcaatc 165000 tggaattaaa aagctggttt attgcgaaac atacgataaa aataaacctg gctgggatga 165060 tattctgcga aatgcgggta ttgaagtgtt taatgttcct aagaaaaact tgaataagtt 165120 aaactgggaa aacatcaacg aattctgtgg tgaataatga aatttcgttt ggtacagctc 165180 acagcaatta gttcttattc taacgagaac atttcatttg ctgtagagta taagaagtat 165240 tttttctcta aatggaaaca gtattataag tcagaatggg tttgtattga tagaccgtat 165300 agttggaaat ctgatttaga aaaatgccaa aaattgcttt ccactcttaa agaacgtgga 165360 acaacccata ttaaaactgt agtaggtaaa taaatgaaac tgacaactga acagaaagta 165420 gcaattcgtg aaattttgaa aactaaattg tccatggggg tttcaaacgt agtttttgaa 165480 aagtctgatg gtactattcg tactatgaaa ggcactcgtg atgcggactt tatgccaacc 165540 atgcaaactg gtaaattgac tgaatctgct cggaaagaat ctactgatat gattccagta 165600 tttgatgttg agcttggtgc atggcgaggt ttttctattg acaaattgat ttccgttaat 165660 ggtatgaaag ttgagcattt gcttcaattt attggtaaat aaatgcttta agaattattt 165720 gttattatta attcatctgt taacaaaaag gaaaaacgat gtctgaagta caacagctac 165780 caattcgtgc tgtcggtgaa tatgttattt tagtttctga acctgcacaa gccggtgatg 165840 aagaagttac agaatcagga cttattatcg gtaaacgtgt tcaaggtgaa gttcctgaac 165900 tgtgtgtagt tcactctgtt ggtcctgatg ttcctgaagg tttctgtgaa gttggtgatt 165960 tgacttctct tccagttggt caaattcgaa acgttccaca cccttttgta gctctgggtc 166020 ttaagcagcc aaaagaaatt aaacaaaaat tcgttacctg tcactataaa gctattccgt 166080 gtctttataa gtgatataaa taataatatg aattgagttt caattctatg tggtagtcta 166140 caactgagag atctgtcgaa agaagatgaa attcagaaga acgtgactac cgagttttaa 166200 tctctaacga gaatttttaa atgattaaac aattacaaca cgctcttgaa ctgcaacgaa 166260 acgcatggaa taatggtcac gaaaactatg gcgcatctat tgatgttgaa gccgaagctc 166320 ttgaaatcct gcgttatttc aaacatctga atcctgctca aactgcatta gctgctgagc 166380 ttcaggaaaa agatgaactt aaatatgcta agccactggc ttctgctgca cgaaaagcag 166440 ttcgtcactt tgtggtaaca ctgaagtaag tatgatgacg ggtttatggt tatcctggtc 166500 gttaaatatt caaaaaccta tgttcccctt gagggcttgc gcaggcaatg ccaataagtc 166560 ctgcattttc atttaaaaga gaatttataa tggcaaaaca agctaaagca aagaaagcag 166620 ttgaaaagaa agttggtgat tctaaacgcg ctggctacaa gcgtgggtcg aactctcgta 166680 tcaatcaaac tgttgagaag atcatgcgcc gagcacgtgc ggttcttcga gatgatgctt 166740 ctcgttttgg taagcagaaa gcataagttg aggactcctt cgggagtcct tttttatttt 166800 ccaaagattg cacaaagttg tttacagtat ggttcctttg tgatagtatt atcttacaca 166860 aacaaaggag aataaaatga aaacaattaa tctgaacgct gcagttaaaa ctaaatgctt 166920 caacggtaaa tatgatgaaa ctatgtggtt cttaatggca gttgaaggtg atattattga 166980 agtagaaaca acagaaggta tgggaacaga tttcaccttt acaattcaag ttcataattt 167040 ctttactggt tggatttatg aattgaacac agtaatcgtt ggaaaaattg aacaaaatga 167100 attgggtgaa tggtattatg ttacagctcg ccaacgtgcc gaacgcttaa ttgagaagat 167160 gaaaaaagtt ggtaaacttg acatgcagca ctggaaagta gtaaaataat tgtttacttt 167220 ggtacaggat atgatattat atacctgtac cgcaattaaa catcttggag aataaaaatg 167280 aactacatca actttgaacg taaatatgtt tctaatggta ttgcaggttc tattgatact 167340 atttgccttt ggaaacatca aaatggatca gtatgcgaaa ttgaacagta tatgacccct 167400 aactacgttt atatgcgatt tgaaaatggc atcacggttt caatcacaat ggaaggttcc 167460 aacttcaaaa tcgctctgga tgatgatttt cgtcaacgcg atttagggac tcatccttgc 167520 tggaatggtg ttaatcgcaa gcttctggtt aaaacttgga ttcgtcatat tctgagtaac 167580 agagctaaac ctgagcatct tgaagcaatc tttgatgtag ttcttaacga atttgatatt 167640 taaaataaaa caggggcttc ggccccttac tgaggaaaat actatgttta tgactactta 167700 ttttgatacc cgcaaaaatt tctgtgaagt ggttttctct aaggcgccta aagaccttcc 167760 tgctcatttg caacctacca gcgaatcgat taaaaactac gttaatgtag tctgtccttt 167820 agagttccgt acagtaaatg ggcgcgatac tttagctatc gctaaactca atcgtgaaat 167880 tgacattgac ccttcaattg cgcgtgaaat taatatttct gatattggcg gcggtaatgt 167940 taaatcgcat ggttttcaga tgaggttcta atgaaattct ttttaggtca aactgttgaa 168000 ttaaagggag ttggtatacc tggattaatt tctaaggttt tacctccatt taaatggagt 168060 ggtgttcaaa taaaagaggc ttatattgtt tcttgggtag atggaaatga agaccttcgt 168120 atgggtgatg aattatctcc tatttacgga ttaaaggaat t 168161

Claims (5)

  1. 서열번호 1로 표시되는 유전체를 갖는 것을 특징으로 하는, 자연으로부터 분리된 미오비리대 박테리오파지 Esc-COP-4 (수탁번호 KCTC 12663BP).
  2. 제 1항의 박테리오파지 Esc-COP-4 (수탁번호 KCTC 12663BP)를 유효성분으로 포함하는, 장침입성 대장균 설사증 방지용 또는 치료용 조성물.
  3. 제 2항에 있어서, 상기 조성물은 사료첨가제, 음수첨가제, 또는 소독제 형태로 사용되는 것을 특징으로 하는, 장침입성 대장균 설사증 방지용 또는 치료용 조성물.
  4. 제 2항 또는 제 3항에 의한 박테리오파지 Esc-COP-4 (수탁번호 KCTC 12663BP)를 유효성분으로 포함하는 조성물을 사람을 제외한 동물에 투여하는 단계를 포함하는, 장침입성 대장균 설사증을 방지 또는 치료하는 방법.
  5. 제 4항에 있어서, 상기 조성물이 사료첨가제, 음수첨가제, 또는 소독제 형태로 사람을 제외한 동물에 투여되는 것을 특징으로 하는, 장침입성 대장균 설사증을 방지 또는 치료하는 방법.
KR1020140192984A 2014-12-30 2014-12-30 신규한 장침입성 대장균 박테리오파지 Ｅｓｃ-ＣＯＰ-4 및 이의 장침입성 대장균 증식 억제 용도 KR101761581B1 (ko)

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MX2017008562A MX2017008562A (es) 2014-12-30 2015-12-28 Novedoso bacteriófago de e. coli enteroinvasiva esc-cop-4 y su uso para inhibir la proliferación de e. coli enteroinvasiva.
PCT/KR2015/014332 WO2016108542A1 (ko) 2014-12-30 2015-12-28 신규한 장침입성 대장균 박테리오파지 Ｅｓｃ-ＣＯＰ-4 및 이의 장침입성 대장균 증식 억제 용도
CN201580071157.6A CN107208067B (zh) 2014-12-30 2015-12-28 新型肠侵袭性大肠杆菌噬菌体Esc-COP-4及其用于抑制肠侵袭性大肠杆菌增殖的用途
RU2017121452A RU2662984C1 (ru) 2014-12-30 2015-12-28 Новый бактериофаг энтероинвазивной e. coli esc-cop-4 и его применение для ингибирования пролиферации энтероинвазивной e. coli
BR112017013245A BR112017013245A2 (pt) 2014-12-30 2015-12-28 bacteriófago esc-cop-4 de e. coli enteroinvasiva inovador e uso do mesmo para a inibição de proliferação de e. coli enteroinvasiva
US15/538,588 US10265356B2 (en) 2014-12-30 2015-12-28 Enteroinvasive E. coli bacteriophage Esc-COP-4 and use thereof for inhibiting proliferation of enteroinvasive E. coli
CONC2017/0006496A CO2017006496A2 (es) 2014-12-30 2017-06-27 Bacteriófago de e. coli enteroinvasiva esc-cop-4 para inhibir la proliferación de e. coli enteroinvasiva

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CO2017006496A2 (es) 2017-09-20
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US20170333499A1 (en) 2017-11-23
US10265356B2 (en) 2019-04-23
CN107208067B (zh) 2020-09-11
CN107208067A (zh) 2017-09-26
RU2662984C1 (ru) 2018-07-31
KR20160080577A (ko) 2016-07-08

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