KR102592424B1 - 병원성 대장균, 살모넬라 또는 시겔라에 대해 사멸능을 갖는 박테리오파지 및 이를 함유하는 항균 조성물 - Google Patents

병원성 대장균, 살모넬라 또는 시겔라에 대해 사멸능을 갖는 박테리오파지 및 이를 함유하는 항균 조성물 Download PDF

Info

Publication number
KR102592424B1
KR102592424B1 KR1020210072550A KR20210072550A KR102592424B1 KR 102592424 B1 KR102592424 B1 KR 102592424B1 KR 1020210072550 A KR1020210072550 A KR 1020210072550A KR 20210072550 A KR20210072550 A KR 20210072550A KR 102592424 B1 KR102592424 B1 KR 102592424B1
Authority
KR
South Korea
Prior art keywords
bacteriophage
coli
kfs
salmonella
pathogenic
Prior art date
Application number
KR1020210072550A
Other languages
English (en)
Other versions
KR20220164159A (ko
Inventor
박미경
김수현
엠마누엘 아데예미다밀라
Original Assignee
경북대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 경북대학교 산학협력단 filed Critical 경북대학교 산학협력단
Priority to KR1020210072550A priority Critical patent/KR102592424B1/ko
Publication of KR20220164159A publication Critical patent/KR20220164159A/ko
Application granted granted Critical
Publication of KR102592424B1 publication Critical patent/KR102592424B1/ko

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/40—Viruses, e.g. bacteriophages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/195—Antibiotics
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/381—Microorganisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Animal Husbandry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 병원성 대장균, 살모넬라균, 시겔라균에 대해 특이적인 사멸능을 갖는 신규 박테리오파지 KFS-EC3 및 이의 조성물에 관한 것이다. 본 발명의 박테리오파지 KFS-EC3는 병원성 대장균, 살모넬라균 또는 시겔라균에 특이적 사멸능을 가지는 넓은 숙주 범위를 가지는 반면, 유익균은 사멸시키지 않고 내산성 및 내열성이 우수하므로, 병원성 대장균, 살모넬라균 및 시겔라균 중 어느 하나 이상의 균에 의한 감염성 질병의 예방 또는 치료에 활용할 수 있을 뿐만 아니라, 항생용 조성물, 사료 첨가용 조성물, 소독제, 또는 세척제 등에도 광범위하게 이용될 수 있다.

Description

병원성 대장균, 살모넬라 또는 시겔라에 대해 사멸능을 갖는 박테리오파지 및 이를 함유하는 항균 조성물{Pathogenic Escherichia coli, Salmonella, and Shigella-specific bacteriophage and antibacterial composition comprising the same}
본 발명은 병원성 대장균, 살모넬라 또는 시겔라에 대해 사멸능을 갖는 신규 박테리오파지 KFS-EC3 및 이를 함유하는 항균 조성물에 관한 것이다. 구체적으로 상기 항균 조성물은 소독제, 세척제 또는 사료 첨가제로 이용될 수 있다.
대장균(Escherichia coli)은 장내세균과(Enterobacteriaceae)에 속하는 그람음성 간균, 통성혐기성 세균으로 포유동물을 비롯한 다양한 동물의 장관 내에 존재하는 정상 세균 총 중 하나이며, 일부 대장균은 병원성 인자(부착 인자 및 내열성 독소 등)을 획득한 병원성 대장균이다.
발병 특성 및 독소의 종류 등에 따라 크게 장관독소원성 대장균 (Enterotoxigenic E. coli, ETEC), 장관침투성 대장균(Enteroinvasive E. coli, EIEC), 장관병원성 대장균(Enteropathogenic E. coli, EPEC), 장출혈성 대장균(Enterohemorrhagic E. coli, EHEC), 장관흡착성 대장균(Enteroaggregative E. coli, EAEC) 등으로 분류될 수 있다. 병원성 대장균이 가축에 감염 시 주 증상은 설사이며 극심한 탈수로 인한 폐사율도 매우 높은 편이다.
특히, 장관출혈성대장균은 베로독소(Verotoxin)를 생성하여 대장점막에 궤양을 유발하여 조직을 짓무르게 하고 출혈을 유발한다. 장관출혈성대장균은 혈청형에 따라 O26, O103, O104, O146, O157 등이 있으며 대장균 O157:H7이 대표적이다. 대장균 O157:H7의 경우 10~100개의 균으로도 병원성을 나타낼 수 있으며, 주요 증상은 동일하게 설사, 복통, 발열, 구토로 심하면 출혈성 대장염, 용혈성 요독증후군, 혈전성 혈소판 감소증 등이 나타날 수 있다.
살모넬라(Salmonella)는 장내세균과에 속하는 그람음성의 통성혐기성 세균으로 사람·동물 및 조류의 장내뿐만 아니라 자연계에 널리 분포하는 병원성 미생물이다. 살모넬라는 인수공통 감염원을 포함하기 때문에 사람뿐만 아니라 돼지, 소, 닭 등을 비롯한 다양한 가축에 감염되어 질병을 발병시킬 수 있을 뿐 아니라 주요 식중독균 중 하나로 감염 시 설사, 구토, 복통, 발열 심하게는 관절염 등의 만성질환을 유발하고 돼지, 소, 닭 등 가축에는 살모넬라증(Salmonellosis)을 유발하여 급성 및 만성 감염증을 발병시킬 수 있다.
시겔라(Shigella)는 장내세균과에 속하는 운동성이 없고 협막이 없는 그람 음성 세균이다. 시겔라는 주로 S. dysenteriae, S. flexneri, S. sonnei, S. doydii가 알려져 있는데 상기 시겔라 균들은 세균성 이질을 발생시키며, 감염력이 강해 매개체 내에서의 증식 없이도 환자나 보균자의 접촉만으로도 전파가 가능하다. 시겔라 손네이(S. sonnei) 균은 나머지 3종에 비해 상대적으로 약한 균으로 평가되지만, 이 균이 대장을 침범할 경우 고열, 설사, 혈변 등의 증상이 나타나면서 심하면 사망에 이르기도 한다.
통상적으로 상기 장내세균과 세균들에 의한 감염 질환의 예방 및 치료를 위해서 실시하고 있는 방법은 백신 및 항생제를 이용하는 방법이 있다. 그러나 미국 질병통제예방센터(CDC)에 따르면 최근 병원성 대장균 O157:H7의 경우 스트렙토마이신(Streptomycin), 테트라사이클린(Tetracycline), 설프이속사졸(Sulfisoxazole), 암피실린(Ampicillin), 날리딕스산(Nalidixic acid) 등, 시겔라균의 경우 시프로플록사신(Ciprofloxacin), 아지트로마이신(Azithromycin), 테트라사이클린, 스트렙토마이신 등, 살모넬라의 경우 시프로플록사신, 아지트로마이신, 테트라사이클린 등에 대한 항생제 내성이 증가하고 있어 항생제를 이용한 치료는 그 효과가 계속 떨어지고 있다. 이러한 항생제 내성 증가의 원인을 항생제 오남용으로 규명하고, 항생제 사용을 규제하기 시작하였다. 또한, 안전한 먹거리와 식품의 안전에 대한 소비자의 요구가 증대되고 있어 점차 항생제 사용이 줄어들고 있다. 이에 따라 항생제를 사용하지 않고 병원성 세균을 제어하는 방법에 대한 요구가 증가하고 있으며, 대안으로 세균을 특이적이고 효과적으로 사멸하는 박테리오파지를 이용하는 연구가 주목받고 있다.
한편, 박테리오파지(Bacteriophage)는 세균을 특이적으로 감염(Infection)시켜 감염된 세균의 성장을 억제하고 저해하는 바이러스로 항생제와 비교해 숙주특이성이 높고 온도, pH에서 비교적 안정하다는 장점이 있다. 또한, 항생제에 대한 감수성에 관계없이 항균 활성을 나타낼 수 있으며 항생제 대비 개발 시간이 짧고 개발비용이 적다.
이에, 본 발명자들은 넓은 숙주 범위를 가지는 박테리오파지를 이용하여 단독처리만으로도 하나 이상의 병원성 세균을 제어할 수 있고, 이에 의한 감염증 예방과 치료에 효과적인 박테리오파지의 분리 및 이의 응용에 관한 기술 개발에 주목하였다. 그 결과, 본 발명자들은 도축 폐수로부터 병원성 대장균, 살모넬라균 또는 시겔라균에 대해 특이적으로 사멸능을 갖는 박테리오파지를 분리 및 정제하였고, 이를 KFS-EC3로 명명하였다. 또한, KFS-EC3에 대하여 박테리오파지의 생활사, 살균 및 정균 효과와 온도, pH에 대한 안정성, 형태학적 특성을 확인하고 유전자 분석을 통해 신규성을 확인함으로써 본 발명을 완성하였다.
대한민국 등록특허 제10-0958139호
본 발명의 목적은 병원성 대장균(Escherichia coli), 살모넬라균(Salmonella) 또는 시겔라균(Shigella)에 대해 사멸능을 갖는 신규 박테리오파지 KFS-EC3을 제공하는 것이다.
상기 목적을 달성하기 위해서, 본 발명은 병원성 대장균, 살모넬라균 또는 시겔라균에 대해 사멸능을 갖는 박테리오파지를 유효성분으로 포함하는 항균 조성물에 대해 제공하는 것이다.
본 발명의 일 실시예에 있어서, 상기 박테리오파지는 박테리오파지 KFS-EC3(Bacteriophage KFS-EC3(수탁번호: KCTC 14585BP)인 것일 수 있으나 이에 한정되는 것은 아니다.
본 발명의 일 실시예에 있어서, 상기 박테리오파지는 접종 후 26시간동안 용균활성이 지속되는 것을 특징으로 하는 것일 수 있으나 이에 한정되는 것은 아니다.
본 발명의 일 실시예에 있어서, 상기 박테리오파지는 -70˚C 내지 60˚C에서 용균활성을 갖는 것을 특징으로 하는 것일 수 있으나 이에 한정되는 것은 아니다.
본 발명의 일 실시예에 있어서, 상기 박테리오파지는 pH 3.0 내지 pH 11.0의 범위에서 용균 활성을 갖는 것을 특징으로 하는 것일 수 있으나 이에 한정되는 것은 아니다.
또한, 본 발명은 상기의 조성물을 유효성분으로 포함하는 항균 소독제에 대해 제공하는 것이다.
따라서, 본 발명은 상기의 조성물을 유효성분으로 포함하는 항균 세척제에 대해 제공하는 것이다.
마지막으로, 본 발명은 상기의 조성물을 유효성분으로 포함하는 항균 사료 첨가제에 대해 제공하는 것이다.
본 발명은 병원성 대장균, 살모넬라균 또는 시겔라균에 대해 특이적인 사멸능을 갖는 신규 박테리오파지 KFS-EC3 및 이를 이용한 향균 조성물에 관한 것으로 다른 유익균에 대해서는 용균성이 없어 항생, 항균 용도로 사용할 수 있으며 사료 첨가제, 소독제, 세척제 등의 조성물로 산업 전반에 널리 사용될 수 있다.
도 1은 박테리오파지 KFS-EC3의 병원성 대장균 O157:H7 ATCC 10536에 대한 용균반 형성 활성에 대해 나타낸 도이다.
도 2는 박테리오파지 KFS-EC3의 전자현미경 영상을 나타낸 도이다.
도 3은 박테리오파지 KFS-EC3의 열 안정성에 대해 나타낸 도이다.
도 4는 박테리오파지 KFS-EC3의 pH 안정성에 대해 나타낸 도이다.
도 5는 박테리오파지 KFS-EC3의 병원성 대장균 O157:H7 ATCC 10536에 대한 저해능 분석 결과를 나타낸 도이다.
도 6은 박테리오파지 KFS-EC3의 흡착능에 대해 나타낸 도이다.
도 7은 박테리오파지 KFS-EC3의 1단 증식 곡선(One-step growth curve) 분석 결과를 나타낸 도이다.
본 발명은 병원성 대장균, 살모넬라균 또는 시겔라균에 대해 사멸능을 갖는 박테리오파지를 유효성분으로 포함하는 항균 조성물에 대해 제공하는 것이다.
일 측면에서, 본 발명은 상기의 조성물을 유효성분으로 포함하는 항균 소독제에 대해 제공하는 것이다.
일 측면에서, 본 발명은 상기의 조성물을 유효성분으로 포함하는 항균 세척제에 대해 제공하는 것이다.
일 측면에서, 본 발명은 상기의 조성물을 유효성분으로 포함하는 항균 사료 첨가제에 대해 제공하는 것이다.
이하, 본 발명의 조성물에 대해 상세히 설명하기로 한다.
본 발명의 신규한 박테리오파지 KFS-EC3는 도축폐수로부터 분리동정된 "박테리오파지 KFS-EC3"이라 명명하고, 2021년 5월 27일자로 한국생명공학연구원 생물자원센터(Korean Collection for Type Culture, 이하, "KCTC"라 약칭함)에 수탁번호 KCTC 14585BP로 기탁하였다.
본 발명에서 용어 "배양"은 미생물을 적당히 인공적으로 조절한 환경조건에서 생육시키는 것을 의미한다.
상기 박테리오파지는 통상의 배지에서 생육 가능하며, 일 예로 뉴트리엔트 브로스(Nutrient broth) 배지에서 배양할 수 있다. 상기 배지는 특정 미생물을 배양하기 위하여 배양대상 즉 배양체가 되는 미생물이 필요로 하는 영양물질을 포함하는 것으로 특수한 목적을 위한 물질이 추가로 첨가되어 혼합된 것일 수 있다. 상기 배지는 배양기 또는 배양액이라고도 하며, 천연배지, 합성배지 또는 선택배지를 모두 포함하는 개념이다. 박테리오파지는 통상의 배양방법에 따라 배양할 수 있다.
배양에 사용되는 배지는 적당한 탄소원, 질소원, 아미노산, 비타민 등을 함유한 통상의 배지 내에서 온도, pH 등을 조절하면서 적절한 방식으로 특정 균주의 요건을 충족해야 한다. 사용될 수 있는 탄소원으로는 글루코즈 및 자일로즈의 혼합당을 주 탄소원으로 사용하며 이외에 수크로즈, 락토즈, 프락토즈, 말토즈, 전분, 셀룰로즈와 같은 당 및 탄수화물, 대두유, 해바라기유, 피마자유, 코코넛유 등과 같은 오일 및 지방, 팔미트산, 스테아린산, 리놀레산과 같은 지방산, 글리세롤, 에탄올과 같은 알코올, 아세트산과 같은 유기산이 포함된다. 이들 물질은 개별적으로 또는 혼합물로서 사용될 수 있다. 사용될 수 있는 질소원으로는 암모니아, 황산암모늄, 염화암모늄, 초산암모늄, 인산암모늄, 탄산안모늄, 및 질산암모늄과 같은 무기질소원; 글루탐산, 메티오닌, 글루타민과 같은 아미노산 및 펩톤, NZ-아민, 육류 추출물, 효모 추출물, 맥아 추출물, 옥수수 침지액, 카세인 가수분해물, 어류 또는 그의 분해생성물, 탈지 대두 케이크 또는 그의 분해생성물 등 유기질소원이 사용될 수 있다. 이들 질소원은 단독 또는 조합되어 사용될 수 있다. 상기 배지에는 인원으로서 인산 제1칼륨, 인산 제2칼륨 및 대응되는 소듐-함유 염이 포함될 수 있다. 사용될 수 있는 인원으로는 인산이수소칼륨 또는 인산수소이칼륨 또는 상응하는 나트륨-함유 염이 포함된다. 또한, 무기화합물로는 염화나트륨, 염화칼슘, 염화철, 황산마그네슘, 황산철, 황산망간 및 탄산칼슘 등이 사용될 수 있다. 마지막으로, 상기 물질에 더하여 아미노산 및 비타민과 같은 필수 성장 물질이 사용될 수 있다.
또한, 배양 배지에 적절한 전구체들이 사용될 수 있다. 상기된 원료들은 배양과정에서 배양물에 적절한 방식에 의해 회분식, 유가식 또는 연속식으로 첨가될 수 있으나, 특별히 이에 제한되지는 않는다. 수산화나트륨, 수산화칼륨, 암모니아와 같은 기초 화합물 또는 인산 또는 황산과 같은 산 화합물을 적절한 방식으로 사용하여 배양물의 pH를 조절할 수 있다.
본 발명에 따른 박테리오파지의 범위는 도축폐수로부터 분리된 서열번호 1로 표시되는 아미노산 서열을 갖는 단백질 및 상기 단백질의 기능적 동등물을 포함한다. "기능적 동등물"이란 아미노산의 부가, 치환 또는 결실의 결과, 상기 서열번호 1로 표시되는 아미노산 서열과 적어도 70% 이상, 바람직하게는 80% 이상, 더욱 바람직하게는 90% 이상, 더 더욱 바람직하게는 95% 이상의 서열 상동성을 갖는 것으로, 서열번호 1로 표시되는 단백질과 실질적으로 동질의 생리활성을 나타내는 단백질을 말한다.
본 발명의 박테리오파지는 이의 야생형 아미노산 서열을 갖는 단백질뿐만 아니라 이의 아미노산 서열 변이체가 또한 본 발명의 범위에 포함된다.
박테리오파지의 변이체란 박테리오파지 천연 아미노산 서열과 하나 이상의 아미노산 잔기가 결실, 삽입, 비보전적 또는 보전적 치환 또는 이들의 조합에 의하여 상이한 서열을 가지는 단백질을 의미한다. 분자의 활성을 전체적으로 변경시키지 않는 단백질 및 펩티드에서의 아미노산 교환은 당해 분야에 공지되어 있다(H.Neurath, R.L.Hill, The Proteins, Academic Press, New York, 1979).
상기 박테리오파지 또는 이의 변이체는 천연에서 추출하거나 합성(Merrifleld, J. Amer. chem. Soc. 85:2149-2156, 1963) 또는 DNA 서열을 기본으로 하는 유전자 재조합 방법에 의해 제조될 수 있다(Sambrook et al, Molecular Cloning, Cold Spring Harbour Laboratory Press, New York, USA, 2판, 1989).
이하, 실시예를 통하여 본 발명을 보다 자세히 설명한다. 다만, 상기 실시예 및 실험예는 본 발명에 대한 예시로 제시되는 것으로, 당업자에게 주지 저명한 기술 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명을 생략할 수 있고, 이에 의해 본 발명이 제한되지는 않는다. 본 발명은 후술하는 특허청구범위의 기재 및 그로부터 해석되는 균등 범주 내에서 다양한 변형 및 응용이 가능하다.
<준비예 1> 병원성 대장균 O157:H7에 감염하는 박테리오파지의 스크리닝 및 단일 박테리오파지의 분리
병원성 대장균 O157:H7에 특이적인 박테리오파지를 스크리닝하기 위하여 소프트 아가 오버레이(Soft agar overlay) 방법을 수행하였다. 소프트 아가 오버레이 방법이란 탑-아가(Top-agar, 0.4% 한천) 배지와 균주를 혼합하여 고체 배지위에 분주 및 배양한 후 박테리오파지가 용균하는 것을 관찰하는 방법이다.
박테리오파지의 스크리닝을 위해 대구광역시 소재의 도축장으로부터 수득한 도축 폐수를 시료로 사용하였다. 구체적으로, TSB 배지(Difco Inc., USA) 225 mL에 샘플 시료액 25 mL, 병원성 대장균 O157:H7 ATCC 10536(Escherichia coli O157:H7 ATCC 10536) 진탕 배양액(OD640 = 0.357) 1%를 혼합하고 이를 37℃에서 160 rpm으로 16시간 동안 배양하였다. 그 다음, 상기 배양액은 6,000 rpm에서 20분간 원심 분리하여 그 상등액을 0.25 μm 필터로 여과하였다. 그 후, TA 소프트 아가(0.4% (w/v) 아가, nutrient broth, NaCl, MgSO4, CaCl2) 4 mL와 병원성 대장균 O157:H7 ATCC 10536 진탕 배양액(OD640 = 0.486) 200 μl을 혼합한 후, 혼합액을 TSA(Tryptic soy agar, Difco, Inc., USA) 평판 배지 상부에 분주하고 상온에서 10분간 방치하여 건조시켰다. 그 위에 상기 도축폐수 시료액 10 μl를 점적한 다음, 37℃에서 18시간 동안 배양하여 투명환(Clear zone)의 형성 여부를 확인함으로써 박테리오파지의 유무를 확인하였다. 투명환(Clear zone) 형성을 확인한 결과를 도 1에 나타내었다.
도 1에 나타낸 바와 같이, 용균반을 형성하는 박테리오파지를 확인하였고, 상기 박테리오파지는 병원성 대장균 O157:H7 ATCC 10536에 특이적인 용균능을 갖는 박테리오파지임을 확인하였다.
그 다음, 용균반 분석(Plaque assay)을 이용하여 단일 박테리오파지를 분리하였다. 소프트 아가(Soft agar, 0.4% 한천) 배지 4 mL와 병원성 대장균 O157:H7 ATCC 10536의 진탕 배양액 200 μl, 상기 박테리오파지의 존재가 확인된 도축폐수 시료액 100 μl를 혼합하고 고체 TSA 평판 배지 상부에 분주한 후, 37℃에서 18시간 동안 배양하여 용균반(Plaque) 형성을 확인하였다. 상기 형성된 하나의 용균반을 멸균된 팁을 이용하여 회수하고 900 μl의 SM 완충용액(50 mM Tris-HCl, 100 mM NaCl, 10 mM MgSO4)에 넣고 실온에서 1시간 동안 방치하여 박테리오파지 용액을 수득하였다. 상기 수득한 박테리오파지 용액으로 다시 용균반 분석을 실시하였다. 이와 같은 과정을 형성된 용균반의 크기 및 모양이 모두 유사하게 될 때까지 최소 10회 이상 반복 실시하여 단일 박테리오파지를 분리하였다.
<준비예 2> 박테리오파지의 대량 배양 및 정제
스크리닝을 통해 분리하여 수득한 박테리오파지를 정제하기 위하여, 병원성 대장균 O157:H7 ATCC 10536을 이용하여 박테리오파지를 대량으로 배양 및 증식 하고 이로부터 박테리오파지를 정제하였다.
구체적으로, 병원성 대장균 O157:H7 ATCC 10536을 37℃에서 12시간 동안 진탕 배양하고, 균주 배양액 1%를 TA 액체 배지(Nutrient broth, NaCl, MgSO4, CaCl2) 3 mL에 분주한 후 2시간 동안 37℃에서 190 rpm으로 배양하였다. 그 다음, 수득한 박테리오파지 1 mL를 분주하고 다시 2시간동안 37℃에서 190 rpm으로 배양하였다. 배양 후, 6000 rpm에서 20분간 원심분리하여 얻어진 상등액을 0.25 μm 필터를 이용하여 여과해 박테리오파지만을 분리하였다. 동일한 방법으로 TA 액체 배지의 양을 8, 20, 200, 800 mL의 순서로 증가시키며 1010 PFU/mL까지 박테리오파지를 증식하였다. 그 다음, 최종 농도가 10%(w/v)가 되도록 폴리에틸렌글리콜(Polyethylene glycol, PEG)과 1 M NaCl을 가하고 4℃에서 16시간 동안 정치한 후, 4℃에서 8,000 rpm으로 20분간 원심 분리하였다. 상등액은 제거 후 6 mL의 SM 완충용액(50 mM Tris-HCl, 100mM NaCl, 10mM MgSO4)을 이용하여 침전물을 수득하였다.
정제된 파지는 CsCl 밀도 구배(1.20, 1.30, 1.40, 1.45, 1.50, 1.70g/mL) 후 4℃에서 22,000 rpm으로 2시간 동안 초고속 원심분리하였으며, SM 완충용액(50 mM Tris-HCl, 100 mM NaCl, 10 mM MgSO4)을 사용하여 투석한 후 사용 전까지 4℃에서 보관하였다.
이와 같이 분리된 박테리오파지를 “박테리오파지 KFS-EC3(Bacteriophage KFS-EC3)"로 명명하고, 2021년 05월 27일 생물자원센터(Korean Collection for Type Cultures, 한국)에 기탁하고, 기탁번호 KCTC 14585BP를 부여받았다.
<실시예 1> 박테리오파지 KFS-EC3의 전체 유전자 분석
분리해낸 박테리오파지 KFS-EC3의 전체 유전자를 분석하기 위하여, KFS-EC3의 DNA를 NORGEN 회사의 Phage DNA Isolation Kit를 사용하여 추출하였다. KFS-EC3의 gDNA를 Illumina platform을 이용하여 유전자 분석하였으며, Open Reading Frames(ORFs)는 Prokka(v.1.12) software를 사용하여 분석하였다.
유전자 분석 결과, KFS-EC3은 이중가닥 DNA를 가지고 있으며 전체 유전체 크기는 167,440 bp의 크기(서열번호 1)로 이 중 GC 함량은 35.5%인 신규의 박테리오파지임을 확인하였다. 또한, 특징적인 기능을 가지는 전체 ORFs의 수는 273개임을 확인하였다.
<실시예 2> 박테리오파지 KFS-EC3의 형태학적 특성
박테리오파지 KFS-EC3의 형태학적 특성을 알아보기 위해, 투과전자현미경 관찰을 수행하였다. 박테리오파지 KFS-EC3 1 mL에 구리 격자(Copper grid)를 침지(Dipping)한 후에, 2% 우라닐 아세테이트로 음성 염색하고 건조하였다. 상기 파지의 형태학적 특성은 전자 현미경 H-7100(Hitachi)를 이용해 100 kV의 전압으로 관찰하였다. 전자현미경 촬영은 경북대학교 공동실험실습관에서 수행하였으며, 그 결과를 도 2에 나타내었다.
그 결과, 박테리오파지 KFS-EC3은 길이(Length) 96.35 ± 6.84 nm, 너비(Width) 86.83 ± 7.52 nm의 머리와 길이(Length) 68.42 내지 133.48 nm 범위의 수축성 꼬리를 가지는 것으로 확인되었다. 이를 통해, 각이 진 머리(Icosahedral head)와 수축성 (Contractile) 꼬리(Tail)를 가진 마이오비리대(Myoviridae)에 속하는 것으로 추정하였다(도 2).
<실시예 3> 박테리오파지 KFS-EC3의 온도 안정성 조사
박테리오파지 KFS-EC3의 온도 안정성을 확인하기 위하여, 다양한 온도 범위(-70℃, -20℃, 4℃, 22℃, 37℃, 50℃, 60℃, 70℃, 80℃)에서 KFS-EC3의 활성을 측정하였다.
구체적으로, 9 log PFU/mL 농도의 박테리오파지 KFS-EC3 용액 100 μl와 SM 완충용액 900 μl를 혼합한 후, 다양한 온도(-70℃, -20℃, 4℃, 22℃, 37℃, 50℃, 60℃, 70℃, 80℃)에서 1시간 동안 각각 정치하였다. 다양한 온도에서 한 시간 반응시킨 KFS-EC3을 용균반 분석(Plaque assay)를 이용하여 박테리오파지 KFS-EC3의 역가를 각각 측정하였으며, 그 결과를 도 3에 나타내었다.
도 3에 나타낸 바와 같이, 박테리오파지 KFS-EC3은 -70℃ 내지 60℃에서 안정하게 활성이 유지되는 것으로 나타났다. 이에 반해, 70℃이상에서는 활성이 낮았다. 따라서, 본 발명의 박테리오파지 KFS-EC3은 -70℃ 내지 60℃에서 사용가능한 것으로 확인되었다.
<실시예 4> 박테리오파지 KFS-EC3의 pH 안정성 조사
박테리오파지 KFS-EC3의 pH 안정성을 확인하기 위하여, 다양한 pH 범위(pH 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)의 SM 완충용액 900 μl와 9 log PFU/mL 농도의 박테리오파지 KFS-EC3 100 μl를 혼합한 후, 1시간 동안 상온에서 정치하였다. 한 시간 반응시킨 KFS-EC3을 용균반 분석(Plaque assay)를 이용하여 잔존하는 박테리오파지 KFS-EC3의 역가를 측정하였으며, 측정 결과를 도 4에 나타내었다.
도 4에 나타낸 바와 같이, pH 3.0 내지 pH 11.0의 범위에서는 박테리오파지 KFS-EC3의 활성이 비교적 안정적으로 유지됨을 확인하였다.
따라서, 본 발명의 박테리오파지 KFS-EC3은 pH 3.0 내지 11.0의 넓은 범위의 pH에서 활성이 유지됨을 확인하였으므로 pH 안정성이 우수한 박테리오파지임을 확인하였다.
<실시예 5> 박테리오파지 KFS-EC3의 챌린지 어세이 분석
병원성 대장균 O157:H7 ATCC 10536 배양액을 TSB 100 mL에 1%(v/v)로 접종하고 배양액의 흡광도 값이 640 nm에서 약 0.486에 도달할 때(108 CFU/mL)까지 37℃에서 190 rpm으로 배양하였다. 그 후, KFS-EC3와 균 배양액을 혼합하여 MOI(Multiplicity of infection) 0.001, 0.01, 0.1, 1으로 맞추고, 37℃에서 110 rpm으로 24 시간 동안 배양하였다. KFS-EC3을 첨가하지 않은 병원성 대장균 O157:H7 ATCC 10536 배양액을 대조군으로 사용하였다. 균의 성장을 640 nm에서 2시간 간격으로 흡광도 값을 측정하여 파지의 용균 활성을 확인하였으며, 결과를 도 5에 나타내었다.
그 결과, MOI와 관계없이 모두에서 대조구에 비해 E. coli O157:H7 ATCC 10536이 유의적으로 저해됨을 확인하였으며, 이를 통해 64시간 동안 KFS-EC3의 용균 활성이 지속됨을 확인하였다. 특히, 가장 낮은 MOI 0.001에서도 용균 활성 및 저해능이 60시간 이상 유지되는 것을 확인하였다. 따라서, 박테리오파지 KFS-EC3는 지속력이 60시간 이상 유지되는 우수한 지속성이 있는 것으로 확인되었다.
<실시예 6> 박테리오파지 KFS-EC3의 흡착능 및 1단 증식 곡선(One-step growth curve) 분석
병원성 대장균 O157:H7 ATCC 10536을 640 nm에서 흡광도 값이 0.486에 도달할 때까지 배양한 후(108 CFU/mL), 병원성 대장균 배양액과 KFS-EC3을 MOI 0.001로 맞추어 혼합하였다. 혼합액을 37℃에서 10분 동안 정치배양하면서 2분 간격으로 수득한 시료에 대해 용균반 분석을 실시하여 상기 박테리오파지의 흡착능을 측정하였으며, 그 결과를 도 6에 나타내었다.
도 6에서 보는 바와 같이, 상기 박테리오파지 KFS-EC3은 접종 후 6분 이내에 박테리오파지의 90%가 병원성 대장균에 흡착한 것을 확인하였다.
1단 증식 곡선 분석을 위하여, 병원성 대장균 O157:H7 ATCC 10536을 640 nm에서 흡광도 값이 0.486이 되도록 배양한 뒤(108 CFU/mL), 4℃에서 1분 동안 16,000 g로 원심 분리하여 상등액을 제거해 균 세포만을 침전시켰다. 그 후, 침전물을 0.5 mL의 TSB 배지에 희석하고 박테리오파지 KFS-EC3을 MOI 0.001로 맞추어 혼합한 후, 37℃에서 상기 실험에서 확인한 침전 시간 10분 동안 배양하였다. 배양된 혼합 시료를 13,000 rpm에서 1분 동안 원심 분리하고, 침전물을 50 mL의 TSB 배지에 희석하여 37℃에서 1시간 동안 배양하였다. 배양 도중 5분마다 시료를 채취하여 용균반 분석을 하였다. 또한 Eclipse period를 구하기 위하여 채취한 시료에 1% 클로로포름을 가한 뒤, 상기 방법과 동일하게 용균반 분석을 하였다. KFS-EC3의 1단 증식 곡선에 대한 결과는 도 7에 나타내었다.
1단 증식 곡선 결과 KFS-EC3의 Burst size는 71 PFU/감염 세포, 지속시간(Latent time)은 20분, Eclipse period는 5분으로 확인되었으며, 이를 통해 본 발명의 박테리오파지 KFS-EC3은 병원성 대장균에 빠른 시간 내에 흡착할 수 있고, 높은 Burst size를 가지는 파지임을 확인하였다.
<실시예 7> 박테리오파지 KFS-EC3의 병원성 균주 특이성 조사
상기 박테리오파지가 병원성 대장균 이외의 다른 병원성 식중독 균주에 대해서도 용균 활성을 나타내는지 확인하기 위해 KFS-EC3의 특이성을 검토하였다.
병원성 대장균을 포함하여 병원성 식중독 균주 36종을 배양한 후, 소프트 아가 오버레이 방법을 통해 용균반의 형성 여부를 각각 확인하였으며, 결과를 표 1에 나타내었다.
상기 실험 결과, 용균반을 형성한 균주를 대상으로 용균반 분석을 이용하여 Efficiency of plating(EOP) 분석을 진행하였다. EOP 값은 아래 식을 통하여 계산하였다.
균주 명칭 용균반
형성 여부		 EOP 값*
E. coli O157:H7 ATCC 10536 O 1.000 ± 0.00
E. coli O157:H7 204p O 0.94 ± 0.01
E. coli O157:H7 O 0.91 ± 0.03
Shigella sonnei ATCC 9290 O 0.85 ± 0.02
Salmonella Enteritidis ATCC 13076 O 0.52 ± 0.02
S. Mission O 0.81 ± 0.01
S. Senftenberg O 0.72 ± 0.05
S. Typhi X NT
S. Typhimurium ATCC 13311 X NT
S. Typhimurium NCTC 12023 X NT
S. Hartford X NT
S. Montevideo X NT
S. Dublin X NT
S. Heidelberg X NT
S. Newport X NT
S. Panama X NT
S. Salamae X NT
Aeromonas hydrophila ATCC 7966 X NT
Bacillus cereus ATCC 13061 X NT
B. cereus ATCC 14579 X NT
B. cereus ATCC 1611 X NT
B. cereus ATCC 21768 X NT
B. cereus ATCC 6633 X NT
Klebsiella pneumoniae ATCC 13883 X NT
Listeria monocytogenes ATCC 7644 X NT
L. monocytogenes KBN10P05037 X NT
Pseudomonas aeruginosa ATCC 10145 X NT
Staphylococcus aureus ATCC 25923 X NT
S. aureus KBN10P01115 X NT
S. aureus KBN10P01328 X NT
S. aureus KBN10P03720 X NT
S. aureus KBN10P05162 X NT
Vibrio parahaemolyticus ATCC 17802 X NT
Yersinia enterocolitica ATCC 23715 X NT
Y. enterocolitica ATCC 55075 X NT
Y. enterocolitica ATCC 9610 X NT
* ≥ 0.50, high efficiency; 0.10 < 0.50, medium efficiency; 0.001 < 0.10, low efficiency; < 0.001, no efficiency. NT, not tested
상기 표 1에 나타낸 바와 같이, 박테리오파지 KFS-EC3은 3종의 병원성 대장균 (E. coli O157:H7 ATCC 10536, E. coli O157:H7 204p, E. coli O157:H7), 3종의 살모넬라균(Salmonella enteritidis ATCC 13076, S. mission, S. senftenberg)과 1종의 시겔라균(Shigella sonnei ATCC 9290)에 대해서 용균 활성을 나타냈다. 이를 제외한 기타 29종의 균주에 대해서는 용균 활성을 보이지 않았다. 즉, KFS-EC3은 병원성 대장균과 상기 3종의 살모넬라균, 1종의 시겔라균에 대해서 용균 활성이 있는 것으로 확인되었다.
특히, 용균 활성이 있는 3종의 병원성 대장균(E. coli O157:H7 ATCC 10536, E. coli O157:H7 204p, E. coli O157:H7), 3종의 살모넬라균(Salmonella enteritidis ATCC 13076, S. mission, S. senftenberg)과 1종의 시겔라균(Shigella sonnei ATCC 9290)에 대해서 모두 0.5 이상의 EOP 값을 나타냈다. 즉, KFS-EC3는 상기 7종의 균주에 대하여 모두 우수한 용균 효율성을 가지는 것을 나타났다.
이상에서 살펴본 바와 같이, 본 발명은 상기에 언급된 바람직한 실시예로서 설명되었으나, 본 발명 분야의 통상의 기술자들은 동일한 기술적 범위 내에서 다른 구성요소를 추가, 변경, 삭제 등을 통하여, 퇴보적인 다른 발명이나 본 발명 사상의 범위 내에 포함되는 다른 실시예를 용이하게 제안할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 본 발명의 범위를 한정하는 것이 아니다.
이하 본 발명의 박테리오파지 KFS-EC3을 포함하는 감염성 질병의 예방 또는 치료용 약학적 조성물의 제제예를 설명하나, 이들 제제예는 본 발명으로 제조될 수 있는 제제예들을 예시적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 제제예에 한정되는 것은 아니다.
<제조예 1> 의약품의 제조
1-1. 주사제의 제조
박테리오파지 KFS-EC3 1 × 106 PFU/mL
주사용 멸균 증류수 적량
pH 조절제 적량
통상의 주사제의 제조방법에 따라 1 앰플당(2 mL) 상기의 성분 함량으로 제조하였다.
1-2. 시럽제의 제조
박테리오파지 KFS-EC3 1 × 106 PFU/mL
설탕 20 g
이성화당 20 g
레몬향 적량
정제수를 가하여 전체 1,000 mL로 맞추어 주고, 통상의 시럽제의 제조방법에 따라 상기의 성분을 혼합한 다음, 갈색병에 충진하고 멸균시켜 시럽제를 제조하였다.
1-3. 정제의 제조
박테리오파지 KFS-EC3 1 × 106 PFU/mL
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mg
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조하였다.
한국생명공학연구원 KCTC14585BP 20210527
SEQUENCE LISTING <110> Kyungpook National University Industry-Academic Cooperation Foundation <120> Pathogenic Escherichia coli, Salmonella, and Shigella-specific bacteriophage and antibacterial composition comprising the same <130> PN2104-180 <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 167440 <212> DNA <213> Bacteriophage KFS-EC3 <220> <221> source <222> (1)..(167440) <400> 1 tccggaacag cagcgcgacg ttatcgctcg tacctttact gagagcttgt aatggcaaag 60 ataattattg aaggttctaa agatgtgtta aatgctttcg ccgagtggtt tagtaattca 120 ggcgaacagc aatttaatga agcgtggacc atgggtgata ttgatggaat ttatcctacg 180 acagaaattt ctgttcaagg atatggcatt catgaaccta ttcgtttagt tgaatatgat 240 ttatgtactg gtgaggaagt caaatatgat tgaagatatt aaaggttata agccacatac 300 tgaagagaaa atcggtaaag tgaatgcaat caaagacgct gaagttcgtt taggacttat 360 ctttgacgct ttatatgacg aattctggga agcactagat aattgtgaag actgcgaatt 420 cgcgaagaat tatgctgaaa gcctcgatca gttaactatt gctaaaacga aactcaaaga 480 agccagtatg tgggcttgtc gtgctgtgtt ccaaccagag gaaaaatatt aatggcacaa 540 ttgctatcag gttttggtac tcattattat gcagcagacc gtcgtgtatc tgttgctcct 600 aagaaaattc aaagtcttga tgacttccaa gaagtagtcc gtaaagcttt ccaggactat 660 gcgcgttatc ttaaagaaga ttcacaggac tgtcttgaag aagatgaaat tgcttactat 720 gagcaacgtc ttgaacagct taaaaacctg catgaggtcc gtgcagaagt ttcaaagtct 780 atgaataaat tgattagatt taaagaataa ttgtttactt ttcctcttga ctgtggtata 840 atttttctat cagttaagag gagaataaca tgactatcaa cacagaagtt tttatccgtc 900 gaaataagct tcgtcgtcac tttgagtcgg agtttcgtca aattaacaat gagattcgtg 960 aggcatcaaa agcagcaggg gtctcatcgt ttcatctaaa atattctcag catcttcttg 1020 atcgtgcaat tcaacgggag attgatgaga catatgtttt tgaattattc cacaaaataa 1080 aagaccatgt tttagaagtt aatgaattcc tgagtatgcc tccacgtcct gatattgacg 1140 aggattttat tgatggggtt gaatatcgtc ctggacgttt agaaatcaca gatggaaatc 1200 tttggcttgg atttacagtt tgtaaaccta atgcgaagtt caaagacccg tcacttcagt 1260 gcaggatggc aattatcaac agtcgtcgtt taccaggaaa ggcttctaaa gcagtaatta 1320 aaactcaatg aggtaagcat gagaaaagca ctactcgctg gtctattggc catttcaatg 1380 atggcacata gctccgagca tactttcagt aatgtccaac tcgataacat gcgctacgca 1440 tatcaattcg gggaacaatt ttctaaggat ggaaaatata aaacacacaa aaatatccac 1500 aagagcggat tgggtcatat aatggctgct attttatggc aagaaagctc tgccggagtt 1560 aatttaaaat ctaaaccaaa gcatcatgcc tatggaatgt tccaaaatta tttgcctact 1620 atgcgagcga gagtcaagga acttggttat aatatgaccg atgctgaaat aaaaagaatg 1680 ttgaataaac ggtccaattc agcttcctgg gcgtacattg aactttctta ttggttaaat 1740 atacataagg gcgatataag aaaggcgata tcctcttata attcgggatg gaatgttaaa 1800 gcaggttcta aatatgcttc tgaagtccta gaaaaggcta attaccttaa aaataataaa 1860 cttttggaaa tagtaaatga ctaaaatttt ggttttatgt ataggattaa tttcattttc 1920 tgcgttagca gatacatcat atactgaaat tagagagtat gtaaaccgta ctgccgcgga 1980 ttattgtggg aaaaataaag catgccaagc tgaatttgcg cagaaattaa tatatgcata 2040 taaagacgga gaaagagata aatcaagcag atacaaaaat gatacattgt taaaacgata 2100 tgctaaaaag tggaatacct tagaatgttc agttgcggag gagaaagata aagccgcttg 2160 tcattcaatg gttgaccgtt tagtagattc ttataatcga ggattgagta ctagatgatt 2220 gtaaaatata tcaagggcga tattgtcgcc ctatttcttc aaggtaatat tattgcgcac 2280 gggtgcaatt gcttccacac aatgggctct ggcgtagcag gtcaattagc aagagcctat 2340 cccaaaattt tagaaataga taaaaccact accgagtacg gttctcgtga taaattaggc 2400 gatatgtcta ttgtttttaa acacagtcct acaggatttg gtatatgcta taacctttat 2460 acgcaatacg aaccgggtcc taatcttgat tatggtgctt tagtaaactg catgatagaa 2520 ttaaatctac aggcagagac ccttttgttt aaaccagtaa tttatattcc acgcataggt 2580 tgcggtattg ctggcggcga ttgggataag gtttctaaat taatcgacat gtttactcct 2640 gatattgatt taatagtggt ggattatgaa agcacattac ccgcatccgt ttgatcctaa 2700 aaacaaagtg gaaattattc gtcaatggga acgcatttgc cgtactaaat gcccaattaa 2760 tagtccacat gatgtagata aagactatat tggaacattc gttgaatata cctttattga 2820 taagaaaggt cgtaaacagc atgtagaaga atattgctta aaggttacat ggttatgagc 2880 caaactagca ttcttaaaaa tgcacattgt gaaaagtgta aatggccagt tgtttttgct 2940 ttatgcaatg atgaaatggc ttgtgatttc gattattggt gctattgctc taataaagga 3000 tgcattaatc ataaaggtga aggattttat tcaggatttt atccttatcc cgattttgtt 3060 aaagaaggta aaccaaaatg aatagttctg agttacaata tgaagtgtta cgtgagcttg 3120 ataatttaat tgaactcgct gttaataaag gttttgccat cggaattggt caaaaagata 3180 ctgatcattt aactatggaa atatttaagc aaaagcgaat tattttaaaa cttctggaaa 3240 ttaatatatg agtctgagta aagaacaaaa agataaattg tttgagctta tccatgaact 3300 tctagatgag catacagaag caaacacctt ttatgatgaa tatggcccgc tatctcccga 3360 tcagcaagaa gaatttgctg accggtttga taagaaagaa aacgaattaa tagcttatgt 3420 gaatacgctt taagaaggtg atatggcgag tttaattttt acttatgcag caatgaatgc 3480 tggaaaatct gcttctcttt tgactgctgc gcataattat aaagagcgcg gaatgagtgt 3540 attagttctt aagcctgcta ttgatactcg cgattctgtc tgtgaagtcg tttctcgcat 3600 tggaattaaa caggaagcga atattattac ggatgatatg gatatttttg agttctataa 3660 atgggctgaa gcacaaaaag atattcattg tgtatttgta gatgaagctc agtttttaaa 3720 aactgaacag gtacatcaat taagccgaat tgttgatata tataatgttc ctgttatggc 3780 ttatgggcta aggactgatt tcgctggaaa attatttgaa ggttctaaag aacttttggc 3840 gattgcagat aaacttattg aactaaaagc agtttgtcat tgtggtaaaa aagctattat 3900 gacagctcga ttaatggaag atggaacgcc agttaaagaa ggcaatcaaa tctgtattgg 3960 tgatgaaatt tatgtttctt tgtgtagaaa acattggaat gaattaacta aaaagctcgg 4020 ttagtgcaaa agttataaat aggtttatct aactaaaggg gtatatatgc tacaattaac 4080 tgaaaagcaa cttcgcaatc ttactgttct tcaattagat gaaattcgta gggaagttgg 4140 aaatatcatt tcagctttgc gtcgagaagt atcactcaac caatctccgg cagactatac 4200 tagattgcga aattttgaaa aataccttga taaagttaag gccgtgcatc ggcataaagt 4260 aaatacagga caaaaatgat aggaggcctt tatggcctta aaagcaacgg cactatttgc 4320 catgctagga ttagcgtttg ctttatctcc accaattgaa gcgaatgtcg atcctcattt 4380 tgataaattt atggaatctg gtattagaca tgtttatatg ctttttgaaa ataaaagcgt 4440 agaatcatct gaacagttct atagttttat gcgaacgact tataaaaatg acccgtgctc 4500 ttccgatttt gaatgtatag agcgaggcgc ggagatggca caatcatacg ctagaattat 4560 gaacattaaa ttggagactg aatgaaattc agcgactttt cacaaagtgg aaaaccttca 4620 aaggcagatg aatacttagg tttattaatg gctgcacaag cttattttca ttctgcgcat 4680 tttgaaacta aaagttatgc tagacacaaa gcatacgatt ttattttctc tgagttgcca 4740 gatttgattg ataaatttgg tgagcaatat ttggggtatt ctggtagaaa atacacgcct 4800 tctattccag atgccagtaa acttcctacc gacacaatta aaatgattga tcgcatacta 4860 gaccaatcta atagcattta taaagaaatg cctccagcca ttcaaagcac gatagatgat 4920 attactggga tgttttacca gagcaaatat cttctttccc tcgaataaca ttagtctcct 4980 tcgggagact tttttcattt taccggttta ctttccattt gagctgtgat actatacaac 5040 tatcggataa agaggagaac atcatgaaaa ttgaagcact caatcaagaa ggaaacatct 5100 acgtcatcat taatggtgat tttttcgtcg acatggatga agttactagt gaagaacttg 5160 tagaagttct taagaaacgc tatagtatgt gtgatgaagc tgcaactcat atggcgtgtg 5220 caatattctc tctttcatat gtggtggaat aatgattagc attgaacaag cagataaaat 5280 taaagaattg gtagctttaa ttcgcaaagc agatgaggaa cttagtgact ttgcttggtt 5340 ttcgtcaggc attgcaaata aaggtgccga agaacttgaa gctaaagttg ataatgctgt 5400 agaagcttta gatatgtttc ttgatgaaat tatcgatcat aatacgagag tttaaatatg 5460 ctaacacgcg aacagtttga aaaaatcatt aaattagcac gtgatattga aatagattca 5520 tatcaattag cagttgagca ttgtgaagga tattcatatg acggtataga aacagctaaa 5580 aaggatttgg ataaatctaa agctaagtta gttcaatatc ttgaaatgat taggtggaat 5640 aatgaaaact gaaaagcaga tgtttttaat gaaactaatt gaagaatatg ctaatgcagt 5700 ttctgactat gaatgttctt ctcgggaaag aggtacagct ttcgcaaagg aagaaatgaa 5760 aatcatggtt gatgctcaca caaagcttca gaattttatt gaaaacgtca tttaatggtt 5820 tacaagttgg caaggttatg gtatagtaat cttgtcaact gccaaggaga agagaatgaa 5880 agttttgttt gctgtgtatg tgatgattca atataattac ccaatgttta cgtataattt 5940 ggtgaacaac attattgata tgattcagag gagtatacaa tgatgagtga ggcgaaacga 6000 ttagtattag aagttagttc actttttggt gaattggcta tagaaaaagt aaataatatg 6060 tatcgtttga cacaagaaga cgatatgcta tattttacgc ctaacgaaat tgttcgttta 6120 acccaaattg aaaattctta tactgataaa attgtaagca ttaatgatga gcataaaatt 6180 catttctatt ctttatgccc aggatttaac attgaaagcg agtcaatatg cttatcaatt 6240 aataattggg atagttttat agataagatt aaatattttt attattctaa tgaaagaaaa 6300 catagtttaa aatggctcaa aaatcgcaat gctattatta ctaacgcttg tgatcagaat 6360 gatgaaactg ttttaaacgt atcaaaatgc tatgaagatg gagatgtctt aactattcgt 6420 caaattgacg attttcgatc gcatatcatt acattaaaca aagacgaagc tattgcacta 6480 aaaacttatc ttgattctgt tattccaact atgatttcaa agtgaggaaa tatgtttatt 6540 tcaagtggaa cagacatgtg ttataaatgc aaagcaaaat taaatgagac gaagaaccaa 6600 tgaattatac taaagtatat aataatttaa ttaagaaagg aaaactcaga aaattagata 6660 aatctaaatt aaatttttat acggaaaagc atcatattat tccatcttgt attggtggta 6720 atgatgattc tgataatcta gttttattaa cagctagaga gcattttata gcccattggc 6780 ttttagctaa aattcattat aattcgcctg gattaattta tgcatggtgg tcattttata 6840 attttggaga agattcttta ggaagaaatc tcaaattaac ttcaagagga tatcagttag 6900 ttagagaaaa attttcaaaa atacattcta atacaatgaa agaaatgtgg aagtctaatg 6960 aatacagaga aaaacgttcg ataacattaa gtcttcctga aataagggct aaaatttcag 7020 aatcccagtt agaagcacaa aacaaaccgg aagttaaaga aaaaatatct aaaggggtga 7080 aagctgcttt taaacgaccg ggagttaaag aaaaacactc tgctgcagtc aaaaaatctt 7140 tgaataattt tgaaactaaa aagaaacaat caaattcatc taaaattaga caaagaactg 7200 gtaaacattg gcaagactat gacttacttt ataagttatg gattaaatta aatagaccta 7260 aacgcgggtc attcggaact tatataagta aattaggata tccaaaatct aattatcatc 7320 gattgattgt acaatttaat gaagattatg aaaggtctaa taatgaaaac tgtagttaag 7380 tcttattttg gtagtcatct ttatggaact tctactccag aatctgatgt agattttaaa 7440 gaaatctttg ttcctcctgc tcgtgatatt cttataggaa atgttaaaga gcatatgagt 7500 aaaaacacta acaacacatc atctaaaaac actaaagatg atattgacca tgaactatac 7560 agtcttaagt atttctttaa attagcagca gatggtgaaa ctgtagcatt ggatatgctc 7620 cacactccac cggaactagt agttaaatct gatttacctg atgtatggaa gtttattcaa 7680 gacaatcgtt ctcgttttta tacgactaac atgaaatcct atttaggata tgttcgcaag 7740 caagcttcta aatacggcgt caagggttct cgtttggccg cattacgtga tgtattgaaa 7800 gtagttaatc aaatccctga gcaatgggtt gattaccaag aagacggttc cattaagcag 7860 cgtcgaacta aagttgaaga tattaagcat cgtcttccag agaacgaatt ttgtgaatgg 7920 gtattccata atcatgagaa aacaggccca caaacgttct acactgtgtt gggtcgtaaa 7980 tatcaaacaa cgctttctct tatcgagctt aaacagtcac tgaacaaatt agatgctgaa 8040 tacggcgaac gcgctcgtaa ggctgaagcg aatgagggca ttgactggaa agctctaagt 8100 catgcttgcc gtggtggact ccaactattg gaaatttaca aaactggtga cttagtctat 8160 ccacttcaag atgctccatt tattctcgac gtgaagttgg gtaaacatcc atttaaaact 8220 gttcaagagt ttttggaaga tgtggtcgat caagtagaag cagcatccat tgaagcttct 8280 aagaacggta tgcagcaaaa agtagatatg ggtttctggg atgacttcct tgagagggtt 8340 tatcttgaaa accatcgaag ttattataaa tgataaggga gccttcgggc tccctttttt 8400 atttcaaaaa tttttcacaa aactgtttac aagcataaag ctttatggta ctatacaact 8460 atcaactgat acggatttgg agaataaaat gaaaactgta actatcaata agggtatcta 8520 cttcggtaaa gaaatctctg gaacttttga gctcttaggc gaatggttcc cagataatgc 8580 tccggtagat gcacaaggag atggtaaagt ttttgttgaa attgacggta aacgtcgcgg 8640 tgtttgggtt tacaaatcag atatttcata tgatggtgta aaagttgaag aagttaaaga 8700 atcatatgaa gatatgaaaa cccgcattaa taaaagattt aatgttatgg gaatgatgac 8760 gaatggtatt attaatggaa atattcgttc attaattatc tctggcgcag caggtattgg 8820 taaaacgtat tctttagata aagctttaaa taaagcaaat gataatggat acattgaata 8880 taaaagcatt aatggtaaaa tctctggtat tggtctttat gagcagcttt ggaataatcg 8940 tgaagagaac tctgtccttt tgattgatga tgtggatgtt ttctctgata tggacattct 9000 taatcttcta aaagctgctc tggacaccgg agagacccgt aaagtctgct ggagcactgc 9060 atcttcttac ttagaagaaa aaggcattga ccgtgagttt gaatttaaag gaacgattgt 9120 ttttatcaca aacgttgaca ttgaccgcga attagaccgt ggtactaaac ttgccccaca 9180 cttacaagca ttagtgtccc gctcagttta tttggatttg ggtgttcaca ctaatgaaga 9240 aattatggtc agggttgaag atgttattct ttcaaccgac atgatgcaaa agcgcggtct 9300 ttctgatgaa gaaacttata aagcattatc atggatgaaa gtcaatgtta atcgtttacg 9360 caatgtttca ctgcgtactg ctctttatct tgctgacttt attatgaccg acaaaaacgg 9420 ttgggaagaa attgctgagg ttactcttct gaaataattc ataagaggac ttctatgaca 9480 aaaaggcagt tcagaaatag attatatgga ctgccattaa aaagatgact agaattaaac 9540 tggtgaatgg aggtagtgat gttatactca aaggctcgtg aaatttacga aactaaaatt 9600 aaagaagcag tatttcagtt cgcaacaacg atgcgatgga caaatgattg ggagtattca 9660 aaaaatcata agaagcccat ggtgacaaga aaggctcata tgttagtgtt aatagaccgt 9720 gagcagatta aagcccgaga agccctccag aatcataaaa aggctgcctt tgaatggttt 9780 atggataaca ctgctcctga gactaagaaa gcagtgagcg catggttcag tggaaaaaat 9840 tgtgaaagaa gtttctttta gtggtttaca agactgttcc tctgtggtac tatacaacta 9900 tcaactacgg aggaacagaa aatgaacgct aaagatattt tcaacttggt aaattacaac 9960 gatggtaaat ttaaatctga agcacaaagc aagttcttta atgacatctc aatcggaggt 10020 gaaatcactg ttgatggagg acaaatttac aaatcccgtt ggaattggat cgttattatt 10080 gatgagattg gtattgtaga aatttacaag aatacgaata aaaatcgtac attacattgg 10140 tctcgtgata ctaacgaaca gtacaaaaag gataaagcat ctaaattatc tcgtgtaact 10200 caagaagata ttgagttcat caagaaagat attttaatgt atgataactt aattgctgaa 10260 gagcaagctg ttattgataa atttgacgag attaaagctt ctcgtgaaat tcctgatttt 10320 atgaaagaat cagtaaatga acgatacact ctcatttcag agcgtattga aacttacaaa 10380 aagcaaagag ctgaacgcca aaatactctt cggaagtttg aagaacggtt aaagacggta 10440 ctcgcataac cgctttatac caaggatggt ataatggttc taagcccttt taattgagat 10500 tattatgaaa cagttgataa ttaaaagatt gaatttattg atatgttgtt tatgtgtagt 10560 agttgcatat ggttattacg caattaatga ttatatgcat tataaagatt atgatgttac 10620 tgtagttaat accattacag gaacacaagg aaaagggtct agtttatcgt ttattgccgt 10680 atatgaactc aaagacggtt atagatttag tgaatatatt tccccagaga tgtattcttc 10740 aatagaaaaa ggtgataata ttactgtaag tttacgtcct tttgacgtaa aacagacact 10800 gtttgataat attgtttggt tctttggaat ggtattagtt caatctgtgt gtggtgctta 10860 tgtagtctgt tcaatcatat tctgtgtatt tagtaaaatt gaaaatgagt gaggaaaata 10920 tgtcagtagt aattaataat gtcaatgcag taattaaatc attagttaat aaaaaaatga 10980 tgaatgaatg gactgtactt cgccgtggag agccagataa attttttcat agatttaacc 11040 caactttgga tttgaatgtt atcgacaaag atgttcatgc agaaatttta gataagttta 11100 aagttgatat tggatttgga ttagaaaaac atttgcagcg aacaagcgga tctggaatga 11160 gtttatctaa tcgcattatg aaagccctta ataaaattgg agcattgtct cgtattaatg 11220 cgagtgaaat ccttcgtaat tataataaag gatatgacct ttatggtcga ctaatgccaa 11280 aattatcatt cgatcaaatg attgcggatt tgtggaaaaa tcaacgacga ttattagcat 11340 taggcgctcg attagctaaa ggtctagata aacaaatgat ttttaagacc aataatacag 11400 aagaccttaa atgctttaaa tttagtattc gcggagatga ttattacatc agagctcgct 11460 ctacagatta tgttaatatg gggcatcatc tctgtttagc ttttgaagtt ttaaaagaag 11520 ctggaacatt agaatattca tctggtgcaa aatgccctat tggttcaagt tgtattttaa 11580 tttatcgccc ggatgaatcc agttcaacta aattgcctac aaaacctgta ccagttcgta 11640 gtaacgaaaa acattctgaa caaattgatt attttaataa acagattgaa gagctgaata 11700 tttctattca acaatatgat gatgaaattt tcagactatc tggactgagt agtaaagcta 11760 aatctgagcg tgaaaagtta attaaaattg tcgatttact taaatcttaa ggaacaccat 11820 gaaaactcgt tctcaaattg aagatatggt tcgtactgcc agcgatactc gtaatgttat 11880 gacatttttg tgtgaaaata atttagaccc tgataaagtt aatcgtgcta ttcatcactt 11940 taaatatcta aatagcagtg aatggttacg tcattttagt aaagcggggt atattacaca 12000 aatgacggct cgtgagcagc tcaccgattt ctgtaaaacc attgattata aaaatcctct 12060 atttgttcaa ggcgttggtc agagtaaggt cgatttatca tctggatttt tcaatccaaa 12120 tcattatcgt attgaatgga gatttattgc tctattccgt aaacaattaa agcaaatttt 12180 gtcgacggct agtcgattaa aaggttctga cattaactta aagaacctga aatttgatgg 12240 ttatactctt cagatggaag taagaccatt aaaagaaaat aacagaactg cacgaattag 12300 ctttaaacct aatacaaaaa attctctttc aatttgcgaa tgccttaaat cgcagttgac 12360 agaagcattt aagtatatgg atgttgttgc cgcagttcag tctaagattt tacctcgttt 12420 tgggcgattt aaattagaca caacaacata tgaacttgat atgatcgttt catttaaata 12480 cgattttttg agaaaggacg aaatcccgca agagaaaaag caggaagtgc aagatatttt 12540 aaatttatcc aattactcat caaacgatcc taaattttgg atgtatagtt caggtaataa 12600 ggatgcatgg aaattcaata aagcgaattt tcttcctgtt gaaaatccga gtcttaaacc 12660 tgttgaaaaa tggcacgcgg atgcgattga gaagtctatc aaggcagtag atgatgaact 12720 cgttaaagca actaatgaag tgttagaagc tgaaaagatg ctagaaaaag ctcaagaaaa 12780 agtcaaaaat ctcacgaagc aacgttctaa actgaacaat gcactaaatg cactaaacta 12840 gtttacttcg ccacaaggat gtggtataat gttcttactt tctactgagg agattaatat 12900 gactcgtaaa cattatattg aatattttga cagtcttatt actaaacacc gtaattatca 12960 gatcggacac agagcagtaa tcaataatat tcttcgtgat ttccttcaat atgttggcca 13020 ggaaaatcat atctgtaaag atactcagaa cgcctattct cattcattgg gcaatttgct 13080 tgaatggttt aaacgttctc gtatgttatc ttctactgta gctcgcgata acattaaaaa 13140 ctttatgaag ccgagcttca ttaaatctgc aacatctata accgatttgg ttgaatttac 13200 tattgttaac gatgttaaaa agacccactt ggctgattgg ttatctacca ttcctgaaac 13260 taaatttgct gataaatttg cttgtcaatt caatgaccaa gtgaatatgc tttttaagca 13320 tgctcgcaaa ctatttactg ctggtgatga ccgtacaaat acagttcatg ttaaggattg 13380 ggttattgct gatgaagtaa cacacaagcc aggtggttca tcagtgttaa ttaatatcca 13440 ggttccttat tattactcac gtaatcttgg caccatgaac tctaaagaaa ttaataagca 13500 caacaaaact attcgttcgt tgtcttataa actccgcatg atgttaaaaa tcatggacgt 13560 agtagaaatg tatgacgaaa tcgaagataa tggttcaatg ttatacagtt cacgtattct 13620 aattaaactg aagaacccta atacatataa gacagttgta aaagagccta aagtagaaaa 13680 gattgataac ttgagcgaag aacgtgaata tctcaatgct cgtttgattg aagttgaagc 13740 ccagattgcc gaacacacca aattgttaaa agctcttaat gcaaaagcaa atggtttacg 13800 taatgctatt gaggtattga aatgaaaaag cgtctattag aagatattgc cgaagcttca 13860 gattttcctg aatggactcc atgcgctggg tttgataaag gactgttagt tactgacgat 13920 ttagatttta aacctccagc ctgggatgct attatggcta tggttgaacg tcgcgaaagg 13980 gcttctaaaa acgttcctaa ttgtcctgaa tgcggtactg aacaggttca attgattaac 14040 tggcgtaaac cggaacttga atataaatgc cgtcattgta aacacaaatt tagcaagcat 14100 gctccggaaa tggttaaatt acctgactct actgagttct ttaaagaact tgcgagtgtt 14160 caaccaatgc ctaataatat tttggattaa aatgaccaag cgtaaagaat atatggagac 14220 tgctgaaaag gcagtccgtg aattagcaat agcgtattat aatgaacatg gtaaatttcc 14280 tgatagatac agcgtgctta aatctgcttt aactcgttca tataaaaata tgctatcaga 14340 agtaagtgat attatataca aacataaaga acaaacgggc caaagtcttg attacgatga 14400 gacttttaaa caagtactag gaattaagga ataatatgtt taaagtatat ggttatgata 14460 gcaacatcca taaatgtgtg tattgcgata atgcaaaacg tcttttgact gtaaagaaac 14520 agccatttga atttatcaac attatgccgg aaaaaggtgt ttttgatgat gagaaaatcg 14580 ctgagcttct aacgaaacta ggtcgtgata ctcaaattgg tttgacgatg ccacaagtat 14640 ttgctcctga tggaagtcat attggtggat ttgaccaatt gcgggaatac tttaaatgat 14700 gctcgaagga actgattata tccatgacta ccgcggaagc gcggtatatg taggtgatga 14760 agtcgcagtt tactatgggt atggaacttt gatgacagcc aaggttattc aaattaaaaa 14820 taaccgtgct aaactcgaag tttattattc taatggtgaa aagtctattt ctaaatggaa 14880 atacggcgat tgtatggtca aactggggta aatatgattt acgatattag cgtatcaaga 14940 actccgtcaa tggttactat tccagctgaa gaattagatc gtcttcagaa aattgaagag 15000 cttctttggg aaattgaatc tgatttgcca tcaggactag aatcctggat tgattacgaa 15060 gaacttaata aattgcgaaa ttaattggtg acaaatgact attgaaaata aattagatgt 15120 tgatgcagtt ctgagcgaaa tcatcgaaga ccatgatgca ttttcagaaa attatgattt 15180 cgatttttcg gattatctta aacctatcga aatagaagac tgggtgcaag atggtaaatg 15240 ccaatatcgt cagtgtgttt attttagtcc aaaacataac gttcatgtgt ctgtaaatga 15300 atctcgctca ggttcttacc attctgactg gtattatgca gttccgactg ttgaactagt 15360 cgagccccgt gaacgtgtag taactcagac aatacgagaa tggattacgc tataaaacct 15420 tggtgggcgg ctagatggga aacagtagag ccagagccgg aagaaccggt ttacactgat 15480 gaagaaacag tatataatga accaacgata aatgacttaa ttgatatgga gatggggcat 15540 gattacagta gataagtggt ttagaattaa tcgtgctgat aaagggctgt gtaattattg 15600 gccggaactt agtgcaggta ctgtctttaa agttcgtgaa cttgcaaaag aatgcgaaga 15660 tgatatagaa cctgatactg gaattattga aattgaactt tctggcggaa aggttattaa 15720 catctacgat aagccaatta cgtattggtg cttgtggaat actgaatccg cagaaaatgg 15780 cgaaattgaa gaagttgtag aacgaactga tcaagttctt cataaaccta aagccgcttt 15840 tcaaggtgaa cgcatttcat atgcattagc taaattagct gcacaagaaa ataacgatgg 15900 ttatgaagga aatttgatgc aagctgcggc agaatacatt gaatggcttg aaactcaaat 15960 ttctttttct gaccgagcgt tttaatactt gaaaataata aataccctta tctatttaag 16020 gtaagggttt tattatgtta ttgactggca aattatataa agaagaaaaa cagaaatttt 16080 atgatgcaca aaacggtaaa tgcttaattt gccaacgaga actaaatcct gatgttcaag 16140 ctaatcacct cgatcatgac catgaattaa atggaccaaa agcaggaaag gtacgtggat 16200 tgctttgtaa tctctgcaat gccgcagaag gtcaaatgaa acacaaattt aatcgttctg 16260 gcttaaaggg acaaggcgtt gattatcttg aatggttaga aaatttactt acttatttaa 16320 aatccgatta cacccaaaat aatattcacc ctaacttcgt tggagataaa tcaaaggaat 16380 tttctcgttt aggaaaagag gaaatgatgg ctgagatgct tcaaagagga tttgaatata 16440 atgaatctga cactaaaaca caattaatag cttcatttaa gaagcagctt agaaagagtt 16500 taaaatgaca attgaaaaag aaattgaagg attgattcat aaaactaata aagacctttt 16560 gaacgagaat gctaataaag attctcgtgt ttttccaact caacgggacc ttatggctgg 16620 tattgtgtct aaacacattg ccaaaaatat ggtcccgtct tttattatga aagcgcatga 16680 aagcggaatt atccatttcc atgatattga ttattcccct gctcttccat ttactaattg 16740 ctgtttagta gatttaaaag gaatgcttga aaacggattt aagctaggca atgcacagat 16800 tgaaactcct aaatcaatcg gcgttgctac tgcaattatg gcacaaatta ctgcacaggt 16860 cgcttcacat cagtatggcg gaacgacttt tgctaatgta gataaaatac tttctcctta 16920 tgttaaacgc acctatgcaa aacatattga ggatgcagaa aaatggcaaa tcgctgatgc 16980 gttgaattat gcccaatcta aaacagaaaa agacgtatac gatgcattcc aagcttatga 17040 atatgaagta aatactctct ttagttcaaa cggacaaacg ccttttgtaa caattacatt 17100 tggtacggga actgactgga ctgaacgaat gattcagaaa gcaattctga aaaatcgcat 17160 taaaggcctt ggtcgcgatg ggataactcc tattttccct aagcttgtta tgttcgttga 17220 agaaggtgtt aatctttata aagacgatcc gaactatgat attaagcagc ttgctctaga 17280 gtgtgcaagc aaaagaatgt atcctgatat tatttcagct aagaacaata aagctatcac 17340 cggttcatct attcctgttt ctccaatggg ttgccgtagt ttcttaagcg tatggaaaga 17400 ttcgactggt aatgaaattc ttgatggacg taataatctt ggcgttgtaa cactgaatct 17460 tcctcgtatt gcgttagatt cttatattgg aacacagttt aatgaacaga aatttactga 17520 attattcaat gagcgaatgg atttatgttt tgaagctttg atgtgtagaa ttagttcctt 17580 aaaaggagtt aaagcgactg ttgctcctat tctttaccaa gaaggtgcat tcggggttcg 17640 tcttaaacct gatgatgaca taattgagtt atttaaaaac ggtagaagtt cagtgtccct 17700 aggatacatt ggtattcacg aattgaatat tcttgtcggt cgtgatattg gacaggaaat 17760 tttgactaaa atgaatgctc gtcttaaaca gtggactgaa agaaccggtt ttgcttttag 17820 cttgtattct acacctgctg aaaacctgtg ctatcgcttc tgtaaacttg atactgaaaa 17880 atatggaagt gtaaaagatg ttactgataa aggctggtac actaacagtt tccatgtttc 17940 agtagaagaa aatatcactc cgtttgaaaa gatttctcgc gaagcaccat atcatttcat 18000 tgcgacaggt ggtcacattt cttatgttga acttcctgat atgaaaaata acctaaaggg 18060 tcttgaggct gtatgggatt atgctgcaca acatttagat tattttggtg ttaacatgcc 18120 agtagataaa tgttttacgt gtggaagtac ccatgaaatg actcctactg aaaacgggtt 18180 tgtttgttct atttgtggag aaactgatcc taaaaagatg aacacaataa gaagaacttg 18240 tggttatttg ggaaatccga acgaacgcgg atttaatctc ggcaaaaata aagaaatcat 18300 gcatagggtt aaacaccaat gaattatgat agattttatc cttgcgattt tgtgaatggc 18360 cctggatgca gggtcgttct tttcgtcaca ggttgtttgc ataaatgcga agggtgttat 18420 aataaatcaa catggaatgc tagaaatggc gttccattca ctggtgaaac actggaacaa 18480 ttaattgaat gtttgaataa tgattatata gaaggattaa ctataactgg aggagaccct 18540 ctctatcctg ataaccgaga tgttgttcat tgcattgttc aaacagtaaa aaatctttat 18600 cccaataaaa gcatttggtt gtggacagga tataaatttg aagatattaa acaactagaa 18660 atgcttaaat atgttgatgt tattattgat gggaagtatg agaaaaatct tccgaccaaa 18720 aagctgtggc gaggatcaga taatcagcga ctttggtcaa ataccgatgg ggtgtggaaa 18780 catgattaaa ttgaattata ttatggacac tataaatgat atgatttttc attttggtcc 18840 agaattttat tcgcaatata gtttagtgct tatcaatgct tggttaatca attaagggta 18900 aaatatgtat aaatttcgta aaggtttagc tgattttctt acaactgtaa cattcttttt 18960 gtttatggca gttggagcta tttttcttat tccttttatt gctatatttt tcgtgattag 19020 tttgatttct ccagaaaaag gactgtcttc tagcgagttc aatgagcgtc tggataaaat 19080 tactaataag ctgaatgctg ctcttagtaa ggaatagttg tgaaacaaaa caagattgaa 19140 gtctatggaa ttccagatga agtaggtcgt tgtcctggat gtcaatcagt tacaaaactt 19200 ctaaaggagc tcaatgctcc ttttactttc tataaagttc ttacaaataa tggtaagatt 19260 gagtatgatc gtccactgat tgtatctctt gctaaacgcg ctggattcac atctcttaac 19320 attcgttatc cagtcatttt cattaatgat tctagacaaa agaacattaa acacttcaaa 19380 gaaactctca tttcacttgg atatgataga gatatcatag aagattaaga cgggccctct 19440 gggcctttct ttctcacatt ctgtatatta ccattctaag ctatcgttcc cttcttatcg 19500 ttccctaaaa taattttcac aaagttgttt acaagaagtt caaactgtgg tattattaac 19560 atatgaattg cctttgagga tttgatatgg ttgataaaga gattaaaagg gatcagtatt 19620 atcgtgttaa tggacatatt gttattgtta cagcagtaga tgatctagat gttcattata 19680 ctgtacttaa atggaataaa cagatgattt gtgatcgtgc cgtatttagt tcagttgcta 19740 aggaaattaa actccatggg taaaacgtat cgtcgtaaag acttaaaagt tcgtgattat 19800 gactatttcg gaaagcgtaa agctcctgat ggtgtaagtc ataaagatat ggttgaaaac 19860 atttttcgct ctgataaatg gcgtagaatg aaaggcattg attcagaagt taaagatgag 19920 ctaaatcgcc aattacgcgg tgaagtaaga aagttgaaaa aatcagttta cattgatgat 19980 gattttgatt ataatacttc tcaacgagtt gctaagcgca aatcaaacga gtgttatcgt 20040 tacagctgag gaaaatatga atatcaaacg catgcttttt aaacaaggat tatacacttt 20100 aaatgttgct ccaaaaggcg atacgaccaa gtggtcagta aatgactgga ttaaatttat 20160 tgatgaaaat ggtaactgga aaatttaaat gaatcccgaa tctaaattat cgcaacgaat 20220 tgctgaagct cgtcgtcagt tctttgaaaa tatgatagcc caaggtattg atgacgaagt 20280 ttttctaaac tggttttgga atcacaaata ttcaaaatgc gcaggagcaa tattaatatc 20340 agccgcaatg atgtatgaag gctggaaggg tgccaaacag tttagctaag ggcttcggcc 20400 ctttttggat aataaaattt taatgtaatt gaggataatg tatgactatt caaattaaaa 20460 acgccatcaa ttcttatgca tatgataaag tagtttcttt gctagaaaaa ggcgatattg 20520 taactcctca aattttggat aaatgggaaa aagagcttca tcagacgatg aaacagaacg 20580 atcagaagat tggacgcaat actgtccgcg aattgttggt tcaatatatc ttgtcagaat 20640 ttgatgttaa agcttttggt gtagaatcta aagcttatca aaagcatgaa atttccgata 20700 aaactattcg tcgcatgaaa aatcaacgca agaaaaaatt tgctgacctg aaaattgcta 20760 aggtatgaat atgaacgaag ctcttattaa cgatttacgt cttgccggat atgaagtaaa 20820 tacaaatggc attggtttaa ctcaaattga aggaaatgga tttatccttg agtatgaatt 20880 tagccaatgg tggttatacg ctaattatgg cgaattgatt gaatacgttg accagtttga 20940 ttcactagat gcagctcttg aagcggctaa gttgatgaat gtatgaaatt aattaatatt 21000 tctattgcta ttgaaaattt tggtattttc tatgttgacc aatacatgaa aatttcattt 21060 ttccaaataa gattggtgtt ggatattggg aaagccatgt ttctgaatta aatgaaagtg 21120 aatatgctag cacacatgaa aagtttttag actttttata tcatgctgat ttaactagtc 21180 attacataga tattcatgaa tttaaaaaga tgatggaaaa agtgttccaa gcatactgct 21240 tacttagata actgatatcc tctatgcttt aagatagatc ttcaaatatt atgatataat 21300 agatctatga attgagctaa gaggtgaaaa tgtcagaaac taagcctaaa tataattacg 21360 taaacaataa agagctttta caagctatta ttgattggaa aacagaatta gcaaataata 21420 aagacccaaa taaagtagtt cgtcagaatg atactatcgg attagccatt atgcttatcg 21480 cagaaggctt atctaaacgt ttcaactttt caggatacac ccaatcttgg aaacaagaaa 21540 tgattgcaga tggtatagaa gcttctatta aggggcttca taattttgat gaaacgaaat 21600 ataaaaaccc acatgcatat ataactcaag cttgttttaa tgcattcgtc caacgtatta 21660 aaaaagaacg taaggaagtt gcaaagaaat atagttactt cgttcacaat gtctatgaca 21720 gtcgtgacga cgatatggtt gcgttagtag atgaaacttt tattcaagac atctacgata 21780 aaatgacgca ttacgaagaa tcaacctata gaacaccggg ggctgaaaag aaaagtgttg 21840 tagacgattc tcctagtttg gattttttat atgaggctaa cgattaacct ctccggattc 21900 ttggaagaaa tacctgaagt tgaagctatt ccctatttac ttaaaatgta tctcagggaa 21960 gttttagctc ttgacattga tattgatcca gaaaatccgt atgataccgc ttttaaatct 22020 aatggtgtag aattaaacta tcggtatcat ttaacagatg atgattttta ttttatatta 22080 gagaaataat atgactgata aacccgaaat taatgatgaa gtggaaaagc ttatttcttc 22140 tattgaagaa aagaaccgtc ttgaagcaga aagaaaagca aataagttat tgtctaaaaa 22200 caaacgcgaa ctgaaccgtc tttataaaca cgctcaaatt gcagctgaaa acaataattt 22260 tgctcaatac gaatatgcta tcaagaaaag tcgggatatt ctaaaacagc catataacga 22320 tgaacttatt agtattcttt ggaagactac tagatcgcag attgaggata tgattgatgc 22380 ttacacacgt aaaattcaag cgtcttaaaa ttaatgcagg atttactgaa tctttgaatg 22440 gtcatctttg cgtgaaaatt tctgaaaaag aataccatga tagttcaatt aaagaagtta 22500 atcctcctat tgtaagagca gaccctaata tgaaagtgtg ggttgattcc tatcaagtca 22560 aaaaatggtg gcaactgtga aagatgaaca cccagacttc tgaaatagat tataataaaa 22620 ttcgttcctc taaagtagaa atgatgaggc gctttaaaga gtctcatgat aaagctaaag 22680 cagaaggaac tataacatat aaacgtataa aatttagaag ctctaatgag cctctgtatg 22740 gtgtattatg cggataggag cttcggctcc tatattgctt tataaatttt tggtaaaata 22800 aaccaaaaca atgaggatat taaatgaaag tatgcatttt tatggctcga ggtcttgaag 22860 gttgtggcgt aactaaattt tctcttgagc agcgtgattg gtttattaaa aatggtcatg 22920 aagtaacttt ggtttatgct aaagataaat catttacccg taattgcgcg catgattata 22980 aatcattttc aattccggtt ttattggcaa aagaatacga taaaacactt aagctggtaa 23040 atgattgtga tattctaatt atcaattcag ttcctgctac ttcagttgaa gaagacacta 23100 ttaataacta taaaaaaatt attgataaca ttaaaccttc ggttcgtgtt gtagtttatc 23160 aacatgacca ttcttctctt tctttgcgcc gaaatttggg attagaagaa actgttcgtc 23220 gagctgatgt tatttttagt cattctgata atggtgattt taataaagtt ctgatgaaag 23280 aatggtatcc ggaaactgtt tctctgtttg atgatattga agaagcaccg accgtatata 23340 actttcagcc tcctatggat attgcgaagg ttcgatcaac ctactggaaa gatgtttctg 23400 aaattaacat gaatatcaac cgttggattg gtcgtacgac tacatggaaa ggcttttatc 23460 agatgtttga ttttcacgaa aaacatctta aacctgcagg actaagtact gttatggaag 23520 gtctggaacg ttctccagcg ttcattccta ttaaagaaaa aggaattcca tacgagtatt 23580 atcgtcttca tcaagtagac caaattaaaa ttgctcctaa tttgccaacg caaattctcg 23640 accgttatgt aaatagcgaa atgcttgaac gcatgagtaa atccggattt ggttatcagc 23700 tgagtaaatt agataaaaaa tatctacaac gttctttaga atacactcat ctcgagcttg 23760 gcgcgtgcgg aacaattcca gtattttgga aatctactgg cgaaaattta aaattccgtg 23820 ttgataatac tccgttaatt tctcatgata gcggtatcgt ttggtttgat gaaaatgaca 23880 tggaatcaac atttgaacgt attaaagaac tgtcatctga ccgagctctt tatgaccgtg 23940 aacgtgaaaa agcttatgaa tttttgtatc agcatcaaga ttcaagcttc tgctttaaag 24000 agcagtttga cattattaca aaataaaggg cttcggccct ttagctttat acggagtttg 24060 atataatgat atttcttgga tatgtgatac tttttctcgc attttatcta ttcactagag 24120 cgtgttggat tgggttcttt agcactccag atggatttat ttcaataatt ttattttgca 24180 tttcgatgac ggttcttgat atatgaaaat tttaaattta ggtgattggc atttaggcgt 24240 taaagctgat gatgagtggg ttcaatccat tcagttggat ggaattaaac aagcaataga 24300 atattctaag aaaaatggaa ttactacatg gattcaatat ggtgatattt ttgatgtgcg 24360 aaaagcaatc acgcataaaa ctatggagtt cgcccgtgaa atagttcaaa tgcttgatga 24420 tgctggtatt accctacata ctgttgtagg aaaccatgat atgcacttta aaaatacttt 24480 aactccaaat gcttctactg agcttttggc taaatatcct aatgttaaag tatatgataa 24540 gcctactaca gtagattttg acggatgttt gattgattta attccttgga tgtgtgaaga 24600 aaatactggt gaaattcttg aacacatcaa aacttcgtct gcttcttttt gtgttggtca 24660 ctgggaactg aatggatttt atttttataa aggaatgaaa tctcacggtc ttgaacctga 24720 tttccttaag acttataaag aggtgtggtc tggtcacttc catactatct ctgaggctgc 24780 taacgtcaga tatattggga caccatggac actaactgca ggtgacgaga atgaccctcg 24840 tgggttctgg atgtttgata cagaaacaga acgaatggaa tttattccaa acaacactac 24900 ctggcatcgt cgaattcaat atccatttaa gggaaaaatt gactataaag attttacaaa 24960 tctatcagta cgtgttatag taactgaggt agacaaaaat ctgacgaagt tcgaatctga 25020 actagaaaaa gttgtgcatt cattacgagt tgtgtcaaag attgataact ctgtcgagtc 25080 agatgatagt gaagaagttg aagttcaatc tcttcagacg ttgatggaag aatacattaa 25140 tgcaattcca gacatcactg attctgaccg cgaagcactt attcaatatg caaatcagtt 25200 atatgtagag gcaacacaat gacttttgat gaatttaaaa atgctatgat gagtcagcat 25260 tttgaatgcg aagtaaaaga tgatattggg cataaagaaa ttattgaata ttggtttgaa 25320 ccgctagagg ttgaagataa ttgtattaaa aaggtcacgg tctgcactga ctgggctgta 25380 tcttttaact tcaacatttt agataatgac acacctaaat cattacaaga tatggctgta 25440 tcttgtatta aggatgcata ttgtgaagtt ttcgacattt gacattaacg atgaattcat 25500 agcaaatatt gattacaccg aagaagattc tagatatgtt ggaataattt atatcacatc 25560 aaaagcagca caaggtgttg tttgcatggc tgaatttgat gaatactttt tagattatga 25620 tgatatgata gaatggtcta aaagatacat taaaaggaat cttttgtgaa gaattttaaa 25680 ctaaaccgag ttaggtatca aaatataatg tcagtaggtg gaaatcctat tgacattcaa 25740 ctagataagg ttcaaaaaac tcttattact ggacgaaatg gcggtggtaa gtctactatg 25800 ttagaagcca tcacatttgg gctttttggc aagccatttc gtgatgtaaa gaaaggtcaa 25860 ttaataaaca gcacaaataa gaaagaactt ttagttgaac tgtggatgga atatgatgag 25920 aaaaagtact atatcaaaag aggacaaaaa ccaaacgttt tcgaaatcac cgttaacggt 25980 acacgtctta atgaatctgc cagcagtaaa gatttccaag cagaatttga acagcttatc 26040 ggaatgtcat atgccagttt caagcagatt gttgtccttg gtacagcagg gtatacccct 26100 ttcatgggtt tgtcgacccc tgcgcgaaga aagcttgtgg aagacctgct tgaggtagga 26160 acattagctg aaatggataa gcttaataaa gcactaatac gtgaattaaa ttcacaaaac 26220 caagtgcttg atgttaaaaa agatagtatt atccagcaaa ttaaaatata taatgataac 26280 gttgaacgcc agaaaaaatt aactggtgat aaccttactc gtctgcagaa tatgtatgat 26340 gatttggcaa aagaagctag aacgctaaaa tcggaaatag aagaagctaa tgaaagatta 26400 gttaatattg ttttggatga agacccgact gatgcattta ataaaatcgg tcaagaagca 26460 tttttaataa aatcaaaaat tgactcgtat aataaagtca ttaatatgta tcacgaaggt 26520 ggattatgtc caacctgttt gtcacaatta agttccggtg ataaagttgt ttctaaaatt 26580 aaagataaag tttctgaatg cacgcattcg tttgaacagc tttcaacaca tcgtgataat 26640 ttaaaagttc ttgttgatga ataccgagat aatattaaaa ctcagcagtc gttggcaaat 26700 gatattcgca ataaaaagca atctctaatc acgacggtag ataaagctaa aaaggttaaa 26760 gcagctatag aaaaagcatc ttctgagttt attgaccatg ctgatgaaat agcactgctt 26820 caagaagaac ttgataaaat tgttaagaca aaaactaatt tagtaatgga aaaataccac 26880 cgaggaattt tgactgatat gctcaaagat tctggtatta aaggtgctat tattaaaaag 26940 tacattccat tatttaataa gcagattaac cattatctta aaataatgga agcggattat 27000 gtgtttacat tagatgaaga atttaatgag acaattaaat cccgtggtcg tgaagatttt 27060 agttatgctt cattcagtca aggtgaaaaa gcacgaattg atattgctct tttatttact 27120 tggcgtgata ttgctgaaaa agtttcaggt gttaaaataa acacattaat tcttgacgaa 27180 gtttttgatt cagcgaccga tgttgaaggt gtaaaagcta tttcaactat tttagatagt 27240 ttaaaaaata ctaatgtttt tgttatttcg catagagacc acgacccaca agcatacggt 27300 caacatcttc aaatgaagaa agttggtcga tttactgtaa tggtttaatt tataagagat 27360 tatgctttaa tttattagag tataatctct atggaggaaa aacatggaat attcaactgg 27420 acagcatcta ttaactattc ctgaaataaa acgatatatt ctgagaaata atttttctaa 27480 tgaagagcat atagttactg aatctatgct taagaatgca tttaaagcag aatatacgaa 27540 aataatgtcc aacagaaatg aagcttggac tgttactgat tattatgact aaaggtgtat 27600 tatgactaaa attactgtga attatactgt tgatgtaaaa gatattcagc caaaacacgt 27660 gcgttctgaa tcaaatccac aaaaccaaaa taaaattcgt cgagcatggg ttttgtctct 27720 ttctgataac gcgatggaag ttattcagaa caaaattaaa tctgcacctg ctcgtcatgc 27780 gtattatgaa gctatcgatc gtgaagtaag taataaatgg attgaactaa tgcgcaaaca 27840 tacggcagaa tctctaaacg ctggtgccaa atttattatg acttcatgtg gtgaacgcct 27900 tgaagatgat tattgcggta atgcagatga acgtctaatt gttgctgctc aaattgttgc 27960 tgaaacaatt gctgccgatt ttaatcgtta attgctttat taaattagtt ataaaattaa 28020 atctcatttg aattgaagga aattacatga aactgtctaa agatactact gctctgctta 28080 aaaatttcgc tactattaac tctggtatta tgcttaaatc cggtcaattt attatgactc 28140 gcgcagttaa tggtacaact tatgcggaag caaatatttc tgacactatt gattttgatg 28200 tagcaattta cgatttgaac ggttttctcg gtattctgtc cctagttaat gacgatgcag 28260 aaatttccca gtcagaagat ggaaatatta aaattgctga tgctcgttca acaatttttt 28320 ggccagcagc tgatccgagt acagtagttg ctcctaataa accaattcca ttcccggtag 28380 catctgttgt tactgaaatt aaagctgaag atcttcagca gctgttgcgt gtatctcgtg 28440 gtctgcagat tgatacaatt gctatcacag taaaagaagg taaaatcgta attaacggtt 28500 ttaataaagt agaagattct gctttgaccc gtgttaaata ttctttgact cttggtgatt 28560 atgatggtga aaatacattt aatttcatta tcaatatggc aaatatgaaa atgcaaccag 28620 gaaattataa acttctgctc tgggcaaaag gtaaacaagg cgctgctaaa tttgaaggtg 28680 aacacgcgaa ttatgtagta gctcttgaag ctgattctac ccacgacttt taataaaggg 28740 cttcggccct ttataattta cactaaaact tgaatgagga aattatgatt actgtaaatg 28800 aaaaagaaca cattcttgaa cagaaatatc gtccatctac tatcgatgaa tgtattcttc 28860 ccgcctttga taaagaaacc tttaaatcta ttacaagtaa aggtaaaatt ccacatatta 28920 ttcttcattc tccttctcca ggaacaggta aaacaactgt agcaaaagca ttgtgtcatg 28980 atgtaaatgc tgatatgatg tttgtgaatg gatcagactg taaaattgat ttcgttcgtg 29040 gtcctttgac taattttgct agtgcagctt catttgatgg tcgtcaaaaa gtaatcgtta 29100 ttgatgaatt tgaccgctca gggttagcag agtctcagcg acatcttcgt tcctttatgg 29160 aagcttatag ttcaaactgt agtattatta ttactgctaa caatattgat ggtattatta 29220 aaccgcttca gtcacgctgc cgagttatta cattcggtca accgactgat gaagataaaa 29280 ttgaaatgat gaagcagatg attcgtcgat tgactgaaat ctgcaagcat gaaggaattg 29340 ctatagctga tatgaaagtt gtagcagctt tggttaaaaa gaattttccg gattttcgta 29400 aaactattgg tgagctcgat agttattcat ctaaaggtgt tttggatgct ggtattttat 29460 cactggttac taacgatcgt ggtgctattg atgatgttct tgaatctctc aaaaataaag 29520 atgttaaaca gctcagagct ttagcaccaa aatatgcagc tgattattcg tggttcgtag 29580 gtaaacttgc cgaagaaatc tatacacgcg taactccgca aagtattatt cgtatgtatg 29640 aaattgttgg tgaaaataac cagtatcatg gaattgcggc taatacggaa ttacatttag 29700 catatctttt cattcaatta gcatgcgaaa tgcagtggaa gtgatatgag cttatttgaa 29760 gatgatattc aattaaacga gcatcaagtt gcttggtatt caaaagattg gacagctgtc 29820 caatctgctg ctgattcttt taaggaaaaa gctgaaaatg aattttttga aataattgga 29880 gctattaata ataaaactaa atgctctatt gctcaaaaag attattcaaa atacatggtt 29940 gagaatgcat tatcacaatt tcctgaatgc atgccagctg tatatgctat gaatttaatt 30000 ggatcaggtt taagcgatga agcccatttt aattatctga tggctgctgt tcctcgtggt 30060 aaaagatatg gtaaatgggc aaaactggtc gaagattcta ctgaagtatt gattattaag 30120 ttacttgcta agcggtatca agttaataca aatgatgcaa ttaactataa atcaattctt 30180 actaaaaatg gaaaacttcc tttagtatta aaagaactaa aaggtttagt cacggatgat 30240 tttttaaaag aagtgactaa gaacgtaaaa gaacagaaac aactcaaaaa actagcattg 30300 gaatggtaaa atgattgaaa ttactcttaa aaaacctgaa gattttctga aagtaaaaga 30360 aactttgact cgtatgggaa ttgctaataa taaagataaa gttctgtatc agtcctgcca 30420 cattcttcag aaaaaaggac tatactatat cgttcatttt aaagaaatgc ttcgtatgga 30480 tggccgtcag gttgaaatga cagaagaaga tgaagttcgt cgtgattcga ttgcgtggct 30540 gttagaagat tggggactga ttgaaatcgt tcctggtcaa agaactttta tgaaagattt 30600 aaccaataat ttccgagtta tttcttttaa acaaaaacac gaatggaaac tcgttcctaa 30660 atatacgatt ggtaattaag gcaaattata tgacacaatc agagattcag tttaccgaag 30720 cttttctaat attcgaaaat ctaaaacaaa catatggttt gtcttctacc caactggttt 30780 ttgacctccc ggaagataaa caaatagaat tccaaaaaga attccaacga ttagtttacc 30840 cgcaccagct cgcatttcag gcgattatca aaaccacaaa taaagatggt aagtccgtta 30900 tatcgcgtcg tacaatagaa atttaaagca aaggggcttc ggccccttat ttggagtata 30960 atatatcaag agcctaataa ctcgggctat aaactaagga atatctatga aagaatttta 31020 tatctctatc gaaacagtcg gaaataacat tgttgagcgt tatattgatg aaaatggaaa 31080 ggaacgtact cgtgaagtag aatatcttcc gactatgttt aggcattgta aggaagagtc 31140 aaaatacaaa gacatttatg gtaaaaattg cgctcctcaa aaatttccat caatgaaaga 31200 tgcacgagac tggatgaagc gaatggaaga catcggtctc gaagctctcg gtatgaacga 31260 ttttaaactc gcttatatca gtgatacata tggttcagaa attgtttatg accgaaaatt 31320 tgttcgtgta gctaactgtg acattgaggt tactggtgat aaatttcctg acccaatgaa 31380 agcagaatat gaaattgatg ctatcactca ttatgattca attgacgatc gtttttacgt 31440 ctttgatttg ttgaattcaa tgtacggttc agtatcaaaa tgggatgcaa agttagctgc 31500 taagcttgac tgtgaaggtg gtgatgaagt tcctcaagaa attcttgacc gagtaattta 31560 tatgccattt gataatgagc gtgatatgct catggaatat attaatctct gggaacagaa 31620 acgacctgct atttttaccg gttggaatat tgagggattt gacgttccgt atatcatgaa 31680 ccgtgttaaa atggttctcg gcgaacgtag tatgaaacgc ttctctccaa tcggtcgagt 31740 aaaatctaaa ctaattcaaa atatgtacgg tagcaaagaa atttattcta tcgatggcgt 31800 atctattctt gattatttag atttgtacaa gaaattcgca tttactaatt tgccatcatt 31860 ctctttggaa tcagttgctc aacatgaaac caaaaaaggt aaattgccat acgatggtcc 31920 tattaataaa cttcgtgaga ctaatcatca acgatacatt agttataaca tcattgacgt 31980 agaatcagtt caagcaattg ataaaattcg tgggtttatc gatctagttt taagtatgtc 32040 ttattacgct aaaatgcctt tttctggtgt aatgagccct attaaaactt gggatgctat 32100 tatttttaac tcactgaaag gtgaacataa ggttattcct caacaaggtt cgcatgttaa 32160 acagagtttt ccaggtgcat ttgtatttga acctaaaccg attgctcgtc gatacattat 32220 gagttttgac ttgacgtctc tgtatcctag cattattcgt caggttaaca ttagtcctga 32280 gactattcgt gggcagttta aagttcatcc aattcatgaa tatatcgcag gaacagctcc 32340 taagccaagt gatgaatatt cttgctctcc gaatggatgg atgtatgata agcaccaaga 32400 aggtatcatt ccaaaggaaa tcgctaaagt atttttccag cgtaaagatt ggaaaaagaa 32460 aatgttcgcg gaagaaatga atgccgaagc tattaaaaag attattatga aaggtgcagg 32520 gtcttgctca actaaaccag aagttgaacg atatgttaag ttcagtgatg atttcttaaa 32580 cgaattatcg aattacaccg aatctgttct taatagtctg atcgaagaat gtgaaaaagc 32640 agctacactt gctaacacaa atcagctgaa ccgtaaaatt cttattaaca gtctttatgg 32700 tgctcttggt aatattcatt tccgttacta tgatttgcga aatgctactg ctatcacaat 32760 ttttggtcaa gttggtattc agtggattgc tcgtaaaatt aatgaatatc tgaataaagt 32820 atgtggaact gatggtgaag atttcatcgc cgcgggtgat actgattcgg tatatgtttg 32880 tgtagataaa gtaattgaaa aagtaggtct tgaccgcttt aaagaacaaa atgatttagt 32940 tgaattcatg aatcagtttg gtaagaaaaa gatggaacct atgattgatg ttgcatatcg 33000 tgagttatgt gattatatga ataaccgcga gcatctgatg catatggacc gtgaagctat 33060 ttcttgccct ccgcttggtt caaagggtgt tggtggattt tggaaagcga aaaaacgtta 33120 tgctctgaac gtttatgata tggaagataa gcgatttgcc gaaccgcatc taaaaatcat 33180 gggtatggaa actcagcaga gctcaacacc aaaagcagta caagaagctc tcgaagaaag 33240 tattcgtcgt attcttcagg aaggcgaaga gtctgtccaa gaatactaca agaactttga 33300 gaaagaatat cgtcaacttg actataaagt tattgctgaa gtaaaaactg cgaacgatat 33360 agcgaaatat gatgataaag gttggccagg atttaaatgt ccgttccata ttcgtggtgt 33420 gctaacttat cgtcgagctg ttagcggttt aggtgtagct ccaattttgg atggaaataa 33480 ggtaatggtt cttccattac gtgaaggaaa tccatttggt gacaagtgca ttgcttggcc 33540 atcgggtacg gaacttccaa aagaaattcg ttctgacgta ctgtcttgga ttgactactc 33600 aactttgttc caaaaatcgt ttgttaaacc gcttgcgggc atgtgtgaat cggcaggtat 33660 ggactatgag gaaaaagctt cgttagactt cctgtttggc tgatagaata aatctaggga 33720 cctccgggtc cctttttcat acaagtaata taaatctata cttaggaaaa atagatgatt 33780 ctaggtccta taaattcatt aaaaattttt tcacaaaact gtttacatcc ctgttcttcc 33840 atggtactat acaactatca actacggagg aacacaaaat gaaaaacatt caaactaatt 33900 ttattcaaaa tttaaaagtt gttaatgcag aaaaagctaa taaactcttt aaagttaaag 33960 aattcgaaaa tgaaggtaat acttatggta ttatagaaca aaatggaatg gtgactggat 34020 tagaaattta taatggcaaa ggaaaatttc agtcacagcc aatttctgtt atgggtgtaa 34080 gaaaaggaat taaaactgaa gatgctttaa aattatttgt taagttttaa gaggaaatta 34140 ttatgaaaat cgctattttg gttattgcgt tgggtcttac tggttgtgta gctcaaggac 34200 cggtagtaaa tcaatctgat gtaggaaaaa ttgtaaactg ttcaagcaaa ttttataatc 34260 ctaacgtcaa gtgttataaa gaagctccaa aacagacagt agaacaaatg caggcgaatt 34320 ttgacgaagc tattcgtcca gacgaatctg ctcaagcata tcgtaattca gatgtaatta 34380 cacgtgaaga aaaaattgaa aactactgcg ccgaactttg ggctaattgg gctaataatt 34440 accaatggcg tactggtaaa aatgctccga tggagtatgt agtgaattct tataattcat 34500 gcgtaaaaaa tttgactaag tgaggaaaac atggaaactt tagtagcagg ttcaattttt 34560 atggttttag tttcaggcgt gttggctatt attatataca tgcttccatg gttcatcgcc 34620 ttgatgcgtg ggtcaaaatc gacagtagga atctttttca cgtctttact gtttaattgg 34680 tcaattattg gttggtttat tacatttatt tggtcaattg cgggtgaaac taaaaaatcc 34740 gcacaaccaa atcaagtaat tatcatcaga gagaaggaat gaaaagcaaa attttagcag 34800 tattgctttt aatcttgatg attataataa gtatatacta tagcgtaacg gttccttgta 34860 tgattccgac acttattata ggttggggtt tattactgtt acaagttaaa tatgaatgta 34920 tcaattgagg tttaaatgat tagtgactct atgacagttg aagaaatccg tcttcatttg 34980 gggcttgcat taaaggaaaa agatttcgta gttgataaaa ctggtgttaa aactattgaa 35040 attattggtg catcatttgt agcagatgaa ccttttattt ttggcgctct taatgatgaa 35100 tacattcaac gtgaacttga atggtataag tctcaaagtc tgtttgttaa agatattcca 35160 ggtgaaacac cgaagatttg gcagcaggta gcatcttcta aaggcgaaat taactcgaat 35220 tatggttggg ccatctggtc agaagataac tatgcccagt atgatatgtg tttagctgaa 35280 cttggtcaaa atcctgattc tcgacgcggt atcatgattt atactcgtcc atccatgcaa 35340 tttgactaca ataaagatgg tatgtcagat ttcatgtgta cgaatacagt acagtacctg 35400 attcgtgata agaaagtcaa tgcggttgtt agcatgagaa gcaatgattg ctgggcaggc 35460 tatcgtaatg attatgcttg gcaaaaatac gtactagata aattagtatc tgatttgaat 35520 gcaggcgacc catcgcggca atataaagca ggttctatta tatggaacgt tggaagtctt 35580 catgtgtacg aaaatcagtt ttatttagtt gaccattggt ggaacaccgg tgagactcat 35640 attgctaaaa aggattatac tggaaaatgg aagtaaatgt gccacatgtt tataagtata 35700 aacatcctaa aactaaaaag tggtatatag gaagtcatga tggtcacaac ccgaattatg 35760 atggttcggg tgtagtttgg caacatgcta aaaagaaata tggaataaaa tcctttaata 35820 aagaaatatt atatgaagga ccaatgttta gacaggttga agaaattatt ttaacttgtt 35880 tagatgctgc taattgtccg gattcatata atttaaagaa tgaagcatgg ggaggaagtt 35940 ttccaggcaa attaaatgga atgtacggta aaaaactatc tccagaagaa agatataagt 36000 gcggaaatgc ctttcgtgga atcaagcgtc ctgatcattc taaaagaatg aaaggcgaag 36060 gtaatccaat gtatggtaaa aatgagcagg catatggaat tataaatcga gccaaggaaa 36120 attctggtaa aacttatgaa gaaatttttg gcgtagaaaa agctaaaata attaaagaaa 36180 cgatgtctaa aaatcgtaaa ggaaaacctc ataatttgat agaaaaaata tgtccgcatt 36240 gcggactaaa aggacgtggg ccaaatatga caagatacca ttttgacaaa tgtaaggcac 36300 ttaaatgatt caattcgtaa ttccaagcta tcaacgcgta ggggcagttt ctgcccttga 36360 tatgtttccg actgattatg aacctcatat tgtagtacgt gaacacgaag aaaaagctta 36420 ttatgacgct tacgggtcta gggctaaaat tataactatt cctgatgatg tcaatggaat 36480 tgctggtact cgtaaagcaa ttactgatat gtatgcaggt caacgaattt ggatgattga 36540 tgatgatact actattcgta tgagttcaat gcgaaagaaa gatgatcgtc gctgcgtaga 36600 taaaattaat cagttaactc gtgaacagtt ctatgaattg attcaatacg ttgaagatgc 36660 catggattgc gggtattatc atggtcatgc tcgtttacca atttttaaaa ttacttcatc 36720 ttggggtaat tatcgtgaaa attcatatgg attcacgaat acttggtatg acctcgggaa 36780 acttacaaca gaacaaattg ggtatggaaa aattgattta tgcgaagata tgtatgcatt 36840 tctcaattta attaatcaag gttatccgca tttggccttg ttcaaatatc tggttgtatc 36900 cggaaaagcg caagctcctg gtggatgtag ttcaattcgt agtaattcaa aacataatag 36960 agctcttgag caaatcaata gagagtttcc agagcaagct cgttggaaaa cttctaatat 37020 tgaaaaacga aaatcattag gtgaagaaga cgagccatta aaggttcttc gcatgtgtgt 37080 ttcgcgcaaa gaaaaatcag aagcgtttca taagtttaat gctattcatc caatagcagt 37140 tgattaatgc ctaaatttat tgtgttataa ttactctatc tttaaccagt gaggaaaata 37200 taatgatgcc tatggaaaaa atgaatgtct attgcagatt taaaatcccg tttgattaaa 37260 gcttccactt ctaaaatgac tgctgaactg actacatcta aattctttaa tgaaaaggat 37320 gtaatccgta caaaaattcc aatgctcaat atcgctattt ccggcgcgat tgatggtggt 37380 atgcagtctg gtttaaccat ttttgcaggt ccttctaaac actttaaatc aaatatgtct 37440 ttgactatgg ttgcggcgta tttgaacaaa tatcctgatg cggtttgttt attctatgat 37500 agcgaatttg gtattactcc agcttatttg cgatccatgg gagttgaccc ggaacgtgta 37560 attcatacac caatccagtc agttgaacaa ctgaaaattg atatggtgaa tcagcttgaa 37620 gctattgagc gcggtgaaaa agttattgta ttcatcgact caatcggtaa tatggcttct 37680 aagaaagaaa cggaagatgc cttgaatgaa aaatctgtgg cagatatgac tcgtgctaaa 37740 tcactgaagt cattattccg tattgttact ccttatttta gtattaaaaa cattccgtgc 37800 gttgcggtta accatacaat tgaaacaatt gaaatgttta gtaaaaccgt gatgacaggt 37860 ggtacaggcg taatgtattc ggctgatact gtattcatta tcggtaagcg ccagattaaa 37920 gatggttctg atcttcaggg gtatcaattt gttctaaatg tagaaaaatc tcgtaccgtc 37980 aaagaaaaaa gtaaattctt tattgatgtt aaatttgacg gtggtatcga tccttattct 38040 ggattgttag atatggctct tgaattagga tttgtggtaa aacctaaaaa tggctggtat 38100 gctcgtgaat ttcttgatga agaaaccggc gagatgattc gcgaagaaaa atcttggcgt 38160 gcaaaagata ccaactgcac tacattctgg ggtcctttat ttaagcatca gccattccga 38220 gatgctatta aacgcgctta tcagttaggt gctattgata gtaatgaaat tgttgaagct 38280 gaagttgatg aattgattaa ctcaaaggtt gaaaaattta aatctccaga aagtaaaagt 38340 aaatcagcag ctgatttaga aactgaccta gatcagttaa gtgatatgga agaatttaat 38400 gaataaagat gatttagatt tagatctaga aattatcgat gaatccccct cttcggaggg 38460 ggaagaagaa agaaaagaac gtctttttaa tgaatctctt aagataatta aatctgctat 38520 ggaaaatgtt atccaggaga ttgtcattaa actagaagac ggttctacac acattgtgta 38580 tgtgacaaaa ttagattggg ttgatggaaa agtcgtaatg gactttgctg ttcttgacca 38640 agaaagaaaa gctgagttag ctcctcatgt agaaaaatgt attacaatgc aactacaaga 38700 tgcgtttaat aaaaggtcaa agaaaaaatt taaattcttt taaggagtaa gtgtggtaga 38760 aattattctt tctcatctca tatttgatca agcttatttt tcaaaagttt ggccatatat 38820 ggattcagaa tattttgaaa gtggtccagc taaaaataca ttcaaattaa ttaaatctca 38880 tgttaatgag taccatagcg ttccatctat taatgcgtta aatgttgcat tagaaaatag 38940 ttcatttact gaaacagaat attctggtgt aaaaacactt atttcaaaac tggccgattc 39000 tccggaagac catagctggt tagtaaaaga aacagaaaaa tatgttcagc aaagggcgat 39060 gtttaacgcc acatctaaaa taattgaaat tcaaactaat gctgagcttc ctccggaaaa 39120 acgaaataag aaaatgccag atgtaggtgc tattcctgac atcatgcgcc aagcattatc 39180 aatttcattt gatagctacg ttggtcatga ttggatggat gactacgaag cacgttggct 39240 atcttatatg aataaagctc gtaaggttcc atttaaactc aaaattctaa ataaaattac 39300 taaaggcgga gctgagactg gaacactgaa cgttttaatg gctggtgtta acgttggtaa 39360 gtcattagga ttgtgttcat tggcagcaga ttatttgcag ctcggacaca atgttcttta 39420 catttccatg gaaatggcag aagaagtctg tgctaaacgt attgatgcta atatgcttga 39480 cgtttctctt gatgacattg atgatgggca tatttcttac gctgaatata aaggaaaaat 39540 ggaaaaatgg cgtgagaaat ctactcttgg acgtttaatc gttaaacagt atcctactgg 39600 tggagcagac gctaatacat ttcgatcgct tttaaacgaa ttaaagctca agaagaattt 39660 tgttccaaca atcattattg ttgactatct aggtatttgt aaatcttgtc gcatcagagt 39720 ttattcagaa aatagttaca caactgttaa agctatcgca gaggaattgc gtgctctggc 39780 tgttgaaacc gaaactgttc tttggactgc agcacaggtt ggtaaacaag cttgggattc 39840 ttctgatgtt aacatgagcg atattgcaga atctgccggt cttccagcaa cagccgattt 39900 tatgcttgcg gtcattgaaa ccgaggagtt agcagctgct gaacaacaac tcattaagca 39960 aatcaaatca cgatacggtg ataagaataa atggaataag tttttgatgg gtgttcaaaa 40020 aggaaatcag aaatgggtag aaattgaaca agattctact ccaactgaag tgaacgaagt 40080 agcaggttca cagcagattc aggctgaaca gaatcgctat caaagaaacg aatccgctcg 40140 agctcagtta gatgctttgg caaatgaatt aaaattttag tttacaagcc gacaagacta 40200 tggtatagta gtcttgttgg ttaaatgagg agatttttat ggaattggta aaggtagttt 40260 ttatggggtg gtttaagaat gaaagcatgt ttactaaaga aatcacaatg atgaaagatg 40320 acgttcaatg ggctactact caatatgctg aagttaataa agcgctagtt aaagctttca 40380 ttgatgacaa gaaagtatgc gaagtggatt gccgaggata atatgcatat tgttttattt 40440 aaacctactc cgtataacgt caggaaaaat actcaattca aagcacttat cgcagatacg 40500 tgggaattgg tattagatat tccagcagaa gaaagccctc catttggtcg agtggaattt 40560 attaagtttg ctgtgcgccc tacaaagcgg cagattcgcc aatgcaaaag atactttcgt 40620 aaaatcgtca agttagagaa acagtttgta acatgtgatt atacagaagt tttaaaatag 40680 tagtttacaa gctgatagag ttgtgttata gtaattctat cagctaatcg cggagaaaag 40740 aaaatgatgc tagttaatag agaatatcag tttaaatcag aagaagattt ggaaaaattc 40800 gcgagtggtt gtgaactgaa tagacgcaca gctaaagtca taggattaaa accctttaca 40860 gttctagatt gtgaagtttc taaattccgc agagggtgtt ctattagcgg tcatgctctt 40920 gttgatggga acacattttt ctttgttttt agcgtcagag aacttttatt gattaacgaa 40980 cttgaggaaa taaaatagtt tacttttgtt ttaaacatgt tattatagac ctatcaaaac 41040 aaacgagtaa atggagaaca aaatgtctaa agtatcaggt tatcaattat taacacaaga 41100 acaacgctca gagatggatt ctttacaaga acggtgtcag catagaaata acgcacttga 41160 ttcattttta ttagttgaat atgaaaactt gtgctctaga cttgaaaaag aatatgttca 41220 tcaacatgaa ggaggagaag aatgaaactg aaatcaccaa ttattgcaat ttgtctgttt 41280 gcttctacta gtgtttactt ttgttgaaaa ttgagatact ataaacataa actactgagg 41340 agattatcat gaaaaaattt atctttgctg caatttttgc tttatcttct tgcgctgctc 41400 agcctgctat ggcaggttat gataaagact tgtgtgaatg gtctatgact gcagatcaga 41460 ctgaagttga aactcaaatt gaagcagata ttatgaatat cgttgagcgt gatcgtcctg 41520 aaatgaaagc tgaagtgcaa aaacagctta agtctggtgg tgtaatgcag tataattatg 41580 ttctatattg cgataaaaac tttaataata aaaatatcgt cgctgaagtg gtaggtgaat 41640 aatgctttac caaatgcaca agtgcaaaga tacatacaaa tataaaggtg cccagtgtta 41700 tatcattaat cgtgaaaacg cgggtccagg tcattcgcat caatcacggt ttgtattcgt 41760 taaaaacaat gaaattatag cagttgctaa ttacatgctt gtgactaatg acgttaatcc 41820 ggtgcctttt acatattata atctaatgga cttggcgtac gattataatt ggtttaatca 41880 tgataatttg gctcatattg aaggcgtagg atttgacatc tcttattcta gttactcgtt 41940 atgtcctatg agcagagctc ataaagagcc tgaatatttt tcatttcgtc ggcgtgtaga 42000 ctttaaacgc agcacagagt atgtcggtgg aatctttgtt aaagataatc gaattactcg 42060 cattatttat cctttgggct gtggtcaaaa agatattgat gttgatttag actttactga 42120 aaacatcatt aatcatattg cttctgtgta ttttgatatt gatcacaaaa ttgtaatatg 42180 tggatatgaa ttaccatcag aaaaacagtc taaagtgaat gtttctattg atgaccaaat 42240 ttttaatgct tttatgaata gaggttaata tgaaaacata taaagaattt atcaaagaag 42300 atatggtagc tggagattca ggtggtaatc ctgaaaatat ctccactgga acaacatcag 42360 gcgctgtagt aaataaaggt cctgaacaga ttcctaaaaa gaaaaaagag gaatctaaag 42420 aaaaagaaga gtaaaaatgt catcaatacc ttggattgat aatgagtttg catatcgtgc 42480 attagctcat ttacctaaat tcacacaagt aaataatagt tcaactttta aattgcggtt 42540 tagatgccct gtttgtggag attcaaaaac cgaccaaaat aaagcccgtg gatggtatta 42600 tggcgataat aatgaaggaa atattcattg ttataactgc aactatcatg caccaatcgg 42660 aatatattta aaggagtttg aacccgattt atatcgtgag tatatctttg aaataagaaa 42720 agaaaaaggt aaaagtcgtc cagtagaaaa acctaaagaa cttcctaaac aacctgagaa 42780 gaaaataatt aaatctcttc cgtcatgcat tagattagat aaattggcgg aagaccatcc 42840 aattataaaa tatgtaaaag ctcgctgtat tccaaaggat aaatggaaat atctttggtt 42900 tacaaccgaa tggcctaaat tagttaatag catagcacca ggaacataca aaaaggaaat 42960 acctgagcct cgtcttgtta ttccaattta taatgctaat ggaaaagctg agtcttttca 43020 aggacgtgca ttaaagaaag atgctcctca aaaatatatc accatcaaag cttatcctga 43080 agcaacaaaa atctatggcg ttgaacgagt taaagatggt gatgtatatg ttctagaagg 43140 acctatagat tcacttttta ttgaaaatgg tatagctatt acaggtggtc aattagacct 43200 agaaattgtt ccatttaaag atagacgtgt gtgggtttta gataatgaac ctcgtcatcc 43260 tgacactatt aaacgaatga ctaaattagt tgatgcagga gaaagggtta tgttttggga 43320 caaatctccc tggaaatcaa aagatgttaa tgatatgatt agaaaggaag gtgcaacccc 43380 tgaacaaatt atggaatata tgaaaaataa tattgcccag gggttgatgg ctaaaatgcg 43440 gctatctaaa tatgctaaga tttaaattaa cccaactaaa gcaaatgcta aatctacgaa 43500 tgtatcaaga gtaattactg gaatattaac accatgagca atagcaattg gtgataaaac 43560 aaaattccaa agtaaaattc ctaccatagc agaaatagta aaagctatac gttttttatt 43620 accttttatg gcattaacaa gtgccattaa tttttgtacc atatgtcctc cttattgctt 43680 tatatattta ttgtataatt aatctactaa cccatgaatt gaaaggaaaa ataatggctt 43740 actttaatga atgttcgcaa ctgatcgaag gcgctgataa agctcaaaat gaatactggg 43800 atattctcgg tgacgaaaaa gacccactgc aagttatgct tgatatgcag aaatctttgc 43860 aggttcgttt agctaaagat aaacctgaat ataaccgtca tcctgatgat ttggctactg 43920 caggcgaagt tgtcgattgg ctgcgtaatc agaaagatta cattgatgat gaattccgtg 43980 aacttctgac ttctcttggt ggtatgagca atggtgaaaa agatgcatct gctgtatgga 44040 agccttggaa gtcgcagcat ggtgaacgtc gtgaaactct aattactgat ttgtcaccac 44100 aagaccaatt ggaaattaaa ttcgaaatga ttgatattct tcatttcgtt ctgaacatgt 44160 tccaaggtct cggtcttacg gctgaagaaa tctttaaact ttattatctg aaaaatgctg 44220 aaaacttcgc tcgtcaagac cgaggttatt aatggctcgt ttaaataaac gccagcttaa 44280 gaaagcccac aagaaacgta ttgaccagct atttaaaaat tatgacaagg agctcgtatg 44340 tgagctctta tctaatcagc tccacaaagc ttacacaatt aaaactggac atatacaatt 44400 aggtaaggtg catgaagaag ccttaaaatt aattatagag cattcaaaat gaaatttgat 44460 ttttatgcat ggcaaaaagc cgggagaccg gctagtcgag catttggttc tgatggatat 44520 aatattagag ctcattggaa gtttaatgct ccagacgatt attctccggt agttattaac 44580 ggtgatgttg ttatattaac taaaattgga tattttagcg ttccacgaga atttattata 44640 aataatcatg taatttaaat aaaaggagaa ttacatggct agtactcgcg gttatgttaa 44700 tatcaaaacg tttgagcaga aattagatgg aaataagaaa attgaaggaa aggaaatttc 44760 tgtagctttc cctctttatt ctgacgttca caaaatttct ggctctcatt accagacatt 44820 cccttcagaa aaagcagcat attctacagt atatgaagaa aatcaacgta ctgaatggat 44880 tactgcaaac gaagatttgt ggaaagtaac tggtgaataa tttcaaggac tcctccggga 44940 gtcctttttt cattttaccg gtttactttc taaaatgagt atggtataat agaattatct 45000 tatagaggag agcactatgt taaatcgctg gattaaacca aatgaagatt tagatattat 45060 catttcacgg catgtaatga agaaatatga actacagcca tggtctacag aagttgttgt 45120 gcattcattt atgatgtacg cagatggttc tgtcgaattt aatgcagaga ttcgatatga 45180 ttatggcgag aagcaagtcg aattcaagag aggctttttg taatgtttat ctttaattgg 45240 tttaaaagct tctttacgga ttttttctct acgactcccg gagaaggtgt agttcctatt 45300 tcaaatgact accttccttt aactgtagtt gaatatgttt atatgggaga tggaacagta 45360 gaagcagtta ctatgactta tgaagaagcc caagaatatt ataaaaatcc ttggcgctgg 45420 tcaacaccta ctacatcatc taatacgcag aatacgcagt ctagttctga ttcatatgat 45480 actaatgttc ctgttcatgt atggacgggc gattcgtgtg gaagttcttg tgattctagt 45540 tgttcatcta catcttgtga ttgaggaaaa ttatggaagc gattttgttt gaaatgtata 45600 ttagcagtaa tagtatgtcg tttgctaaag acgttccaat taccgtagca gtaatgattg 45660 ataagggtta ttgtgaccca atgtatctcg tagaaaattt tgtttcaatg ccagttccag 45720 aagatgctga aataaaactt aaaaagatcg gaattattga aactgtacca aatattccat 45780 ttagagcaat tgaagcattt actaaatccg aatacattaa tgttagcgca gaacaatata 45840 atgataaacc tttatctttc tattcgtatg attcagtata tagctggaaa atagataaag 45900 gaaataaatt tataattgtg agtgaagatg ctttatcata ctttatttct tctgtatgga 45960 atagtttaca tccaaatttg ctaaaaattc atgaatttga tgatgctcct actgttgttt 46020 taggtaaaac aaatgaaagt tctgaagaaa atgtttgaat ggttcaatag accaaactca 46080 atgtacattg acgatggttg ggttgaacaa gcaaataaag aaatgcagaa cgaatcagaa 46140 gaatggatga aatcaatgat tagtgctgag aaagaaaaga aattagaacg ctcagcgctt 46200 aaattgatga gagacatcta tggggataaa tcatgaacag agatatgacg ctagaagagg 46260 ccaaggctaa agcaaatgaa gcattggatt tgcttcttaa aattggcagt aaaatgatgg 46320 aagaaaatga gaaatatatc caggaaaaca aaattcctga cggcccatta gtaggcaaga 46380 ggaaatcaca tgattgaagt agcaaaacat tattcaatag aatttatgtc caaagaaggt 46440 aaatcagtaa atacacttga taaaaagtgc tcattaatta ttcctttagc agaaaatccg 46500 gatcttttaa ttaaagatat aaaagaaaga aaatatccag aaaatgttat tctaattata 46560 aagcatactg aagatatttt gcagaatact gattcaccat tttcttcttc tgaagcttta 46620 actattaaag gctataaaag agctcatgaa tatggtcttt ttgacctgtt tgaagacgat 46680 aaagttaaat tagttagtca accttctaaa agtaaaacat tcattattga agatattaaa 46740 gatataaatg cattcgttaa gatggtctgg gctcattttg atgttggact acgctggaga 46800 atgtccgaag aagaaagaaa gattattgaa gctaatcgtc aatttggttt ttatcgctag 46860 gaattaatat ggatttattt gagatgttag aagataatca ttctacgaat atccagaatg 46920 attctagtga ttataagaaa gagtaccgta tagtattaca gaattatgga attgaagccc 46980 cagatgctct tctagaagaa ctagcttcat accatcttga ccctccgccc tgggctccct 47040 gggcaaaata attcaaaaag ttgtttactt tccttcctaa caatgatatg atagcttctg 47100 aagtatatgg aggctatcat gattattaat cttgcagatg ttgaacagtt atctataaaa 47160 gctgaaagcg ttgattttca atatgatatg tataaaaaag tctgtgaaaa atttactgac 47220 tttgagcagt cagttctttg gcaatgtatg gaagccaaaa agaatgaagc tcttcatcgg 47280 cagttgaata aaatcattaa aaagcattta actgaatcac cttatcagtt atatcgtggt 47340 atatcaaaat cgacaaaaga actcattaaa gatttacaag ttggagaagt gttttcaacg 47400 aacagagtag attcatttac tactagtttg catacagcgt gttctttttc ttatgctgaa 47460 tatttcactg aaataatatt tcgcttaaaa actgataaag cttttaatta ctctgaccat 47520 atcagcgata ttatactttc ttctcctaat actgagttta agtatacata tgaagatact 47580 gatggattag attcagaacg tactgataac ttaatgatga ttgtgcgtga acaagaatgg 47640 atgattccaa ttggaaagta taaaataact tctatttcaa aagaaaaatt acacgattcg 47700 tttggaacat ttaaagtgta tgatattgag gtagttgaat gaaatattca gcaatgcaat 47760 taaaagattt taaaatcaaa tcaatggatg catctgtgcg tgcttctatt cgtgaagaat 47820 tactttctga aggatttaat ttatccgaaa ttgaactttt aattcattgt attactaata 47880 agccagacga tcattcttgg ttaaatgaaa taatcaaatc tcgtttggtt ccaaacgata 47940 aacctctttg gagaggtgtt ccagttgaga ctaaacaggt gttaaatcaa ggaattgata 48000 ttattacatt tgataaagta gtatcagctt catatgataa aaatatagct ctgcattttg 48060 cttctggatt agaatacaac acgcaagtta tttttgaatt caaagctcct atggtgttta 48120 atttccagga gtatgctata aaagctttac gctgtaaaga atatagccca agttttaaat 48180 ttccggatag ccaccgttat cgtaatatgg aattagtttc agatgaacaa gaagtaatga 48240 taccagctgg aagtgtattt agaattgcgg atagatatga gtataaaaag tattcaacat 48300 acactatcta tactcttgat tttgaaggat ttaatctata atggaaggac ttagattcat 48360 tataccatga aagttttaaa gcatttttca taaagttgtt tacaagctga agtaaaaatg 48420 ttatagtata agtagttaac ccgtccgtga gaaaaatatg aaactgtcta aaaatcaaat 48480 tcgtaaaatt acacgtcgtt tagagcatac tcaggcatct gccaaaagac gttctaaaga 48540 ttttaactta gacttcaatt acattaagaa cattttagat cagaaagttt gcgcttactc 48600 gggagaacct tttgataatc gtattgaagg agagaaatta tcattagaac gttttgataa 48660 taacgttgga tacattaaag ggaatgttat tgcagtaaag aaaaagtaca atacatttcg 48720 ttctgattat actttagaag agttgattga aaagcgtgat ttgtttgctt tacgaattgg 48780 tcgttcatct gcgaaaaaag ttcataaact aaatttagat gaaaagaaat gggctaaaat 48840 caaaaagact tataatcaaa ttaaagctat acagaaaaaa cgcgaaaacc gaattgagca 48900 catttctcag ctttctaaat caaaacaaac ttctgacgtt aagctaacga ttatagcact 48960 taaagctcgt attgatggtt ctcgtatagc agaaggcgct gaagttgtta aattgaacgt 49020 tcttcttaaa ggctcggatt ggaaaactgt gaaaaagttg tcagaagcag aaatgcaata 49080 tgatatgtgt gataaaatta ttcaaggtgt agagcggtat caaaacttgt cttttattga 49140 taaacttaaa ctgaaaagag gatacccgct aaattgttca atttttaaac ttatccgagg 49200 ataatatgtt ttatgtatat gcgatagtgt atagagataa agatggattt gcggtgcctg 49260 ttcctcttga tgaacatcgt cctgctgtat tttttgaaag ggagattgcc gataaagttt 49320 ttacaactct taaagagcag tatcgactag ctttaggtat gggaattccg agattagttg 49380 agactccacg caagttttgg tttaataaaa tagaagttaa acatgttaag cctgatgtag 49440 acacacaaag attatatcgg cgaattttag atactgggcg tattgttagt ataccaattg 49500 cagggaattt acgatgacat ttgatgattt gacagaaggc cagaaaaatg cctttaacat 49560 cgttatgagg gctattaaag aaaagaaaca tcatgtaact attaatggac ctgctggtac 49620 tggtaagact actcttacta agttcatcat tgaagcctta atatctacag gcgaaactgg 49680 cattatttta gcagctccta ctcatgcagc taaaaagatt ctttcaaaac tgtcagggaa 49740 agaagcgagt actattcaca gtattcttaa aattaaccca gtaacatacg aagaaaacgt 49800 tctttttgag caaaaagaag taccggattt ggctaaatgc agggtattaa tctgcgacga 49860 agtgtcaatg tatgatagaa agctatttaa aattttgctt tcaactatcc cgccatggtg 49920 tactataatt ggaataggag ataataagca aattagacct gttgacccag gagaaaacac 49980 tgcttatatc agtccgttct ttacgcacaa agacttttat cagtgtgaac tcactgaagt 50040 taaacgcagt aatgctccta ttattgatgt agctactgat gttcgcaacg gtaagtggat 50100 ttatgataaa gttgttgacg ggcatggagt acgtggattt actggtgata ccgctttacg 50160 cgattttatg gtaaattatt tttcaattgt aaaatcacta gatgatttgt ttgaaaatcg 50220 cgtaatggca tttacgaata aatctgttga taagttaaat agcattattc gcaaaaagat 50280 ttttgaaact gataaagatt ttatcgttgg tgaaattatt gtaatgcagg aaccattaat 50340 taaaacatat aaaattgatg gaaagcctgt gtcagaaatt atttttaata acggacaatt 50400 agttcgtatt atagaagcag agtatacatc aacatttgtt aaagctcgtg gtgtttctgg 50460 agaatatcta attcgtcatt gggatttaac agtagaaact tacggcgatg atgaatatta 50520 tcgtgaaaag attaaaataa tttcatctga tgaagagctg tataagttta acttattttt 50580 aggtaaaacg gcagaaactt ataaaaattg gaataaaggt ggaaaagctc catggagtga 50640 tttttgggat gctaaatcac agtttagtaa agtgaaagca cttcctgcat caacattcca 50700 taaagcacag ggtatgtctg tagaccgtgc ttttatctat acgccttgta tccattatgc 50760 agatgctgaa ctagctcagc agcttcttta tgttggtgtc actcgtggtc gttatgatgt 50820 gttttatgtg tgaggatata tgattaacat caattcaaaa tatttaaatc gtctaataga 50880 tggtataaag aagcatactg ataagcaaga taatctcagt gttatggtaa caggagctga 50940 gctcctttat aagcttaatc ttataagtga tactatactg gcaattaaac gaattgaaaa 51000 acaatcatat catagtaata cagatacggt aattacatta gatgaaagtg tctgtaagct 51060 gctaattaaa tttgaggaag ctattcgtgg aaataactaa agaccagttt tatctacttc 51120 aagataaagt gagcgaaatt tatgaaatag cttatagtaa aaatcgcaaa aacgtgaaaa 51180 ttgaatcggg aaagttaatg cttcaattgg aagaaattga acgagatttg attgcattag 51240 aattcttttg tggtgaagtg aaaactgtta caatcagtga ttatgtttta ggcgaaatta 51300 gccatcttta taaggcggtt attaatgatt gaattaagtt tgcatcagtt taaatctctt 51360 atggcaaatg ttaaagctgt cattcaagaa aattcaggac ctgataatgt tactattcgc 51420 gaaaaagctt taaagatagt atacagtctt gaagagatac aaaaagatat tgaatctatg 51480 gcaaaattta ttgatgagcc cattaataaa gtttatatcc aagattatac tgtaggtcaa 51540 attcgcgatt tagcgaggaa aatttaatgt ttgattttat tatagatttt gaaacaatgg 51600 gaagcggtga aaaagcagct gttattgact tagctgttat tgcctttgac cctaacccag 51660 aagtagttga atcctttgat gaattagttt cacgcggcat taaaatcaag tttgatttaa 51720 aaagccaaaa aggacatcgt ctttttacta aaagcactat cgaatggtgg aaaaatcagt 51780 ctcctgaagc tcgaaaaaat attgcgccat cagatgaaga tgtaagcact atcgatggta 51840 ttgcaaaatt taatgattac atcaatgcac ataacatcga tccttggaaa tctcaaggct 51900 ggtgccgtgg aatgtcgttt gattttccaa ttttagtcga tctcattcgc gatattcagc 51960 gtcttaacgg cgtttctgag aatgaacttg acacatttaa gttagaacca tgtaaattct 52020 ggaatcaacg tgatattcgt actagaattg aagcactttt gcttgttcgt gatatgacca 52080 cgtgtcctct tccaaaggga actttagatg gattcgttgc acatgattct attcatgact 52140 gtgcgaaaga catcctgatg atgaagtatg ctttgcgata tgctatgggt cttgaagatg 52200 ctccgtcaga ggaagattgc gatcctctat cccttccaac aaaacgataa aaagttgttt 52260 acttcctcgg ttagttatgg tattataaca ccatagctac tgaggataat aaaatgaaaa 52320 tttatcgtgt tgaacttctg aacgttgttc atgcaggtga ttttagcgta gtacctcgta 52380 gtgcagtggt agctatttta aattaatagt ttacaaactc ttgggaccag agtataatgg 52440 tcctgtggag tataaaatct ttttaacaag tgagagataa ctatgattat taatattggt 52500 gaaattgctc gtgtatctga taaatcacgt tctaaagcag caggaaaatt ggtcgaaatt 52560 gtaagcattc agcttaaaca tggtgttaaa gatgaagatt ctgaagtaaa agtgcgtatc 52620 attcctaaag atggaaagtc taaacctcag tttggctatg ttcgtgcgaa atttcttgag 52680 tctgcgtttt tgaaagctgt tcctgctaaa ggaattgaaa cgattgatac ttcgcatgta 52740 ggtgtagact ttaagtggaa actcggtcag gctattaagt tcattgctcc ttgtgaattt 52800 aaatttatca aagatgatgg tgtggctgtt tatactcgtg ctatgtgcgc atacattacc 52860 gatcagtggg tagaagacgg tgttaagctg tataatgtgg tatttttagg aacatacaaa 52920 gtcattcctg aaagctggat taaacactat agcaaagctc tctatgcata aagtttaagg 52980 aattattatg aatattgtaa atctctctac tggtgattac gtatatgtat ccgttgcttc 53040 tcgttctaaa gttgcaggaa agctggttga agttctttct ataagtgatc cagtttttgg 53100 tcgtaacgag gttaaagttc gtgacgctga tggtaacatt ggttatgtct caagccaatt 53160 cattgagcca gaagaaagcg catttaaatg gaaactttgt tcttcagttt tagttgtaaa 53220 agaccataaa ttcacaattg cggcggcaac agatggaact ccgtggacaa cgaatcaagt 53280 cgctggtttt atttcggacc gttgggtaga agatggcgtc aagctctaca atattgtttt 53340 tgcaggccag ttcatggtag ttcctgaatt ccaaattaaa aaatggtgcg aagcagcttt 53400 agcataaatt tgcataaagt ttaaaatttt ttcataaaac tatatacatc agtagttgat 53460 tatggtacta tatcaatatc aactactgat acagaaaaca acttggagaa taaaatggaa 53520 aacttaatta tcatcgaaca atctttcaac gattatggta tggcttatgg ttatcgtgcg 53580 ataatggaag attctcgtgg atgtgttatc gatattgctg aatgtaaaga tttactgcag 53640 cttttgaaga ttgttcgtaa aaattgggat tgtgaaaaca ttaaagttcg tgttgtaacg 53700 gaagaagaaa ctgtttttca tgatgtaaaa tttgccaaag gtgctgctac tcttctgaaa 53760 cgcattgctc cactgttcaa ttaatgagga aattgtaatg aaacgtaaaa ttgttcagaa 53820 ctgcactaat gacgaatttg aagatgtatt attcgatcca gatttggtag tagttcaaaa 53880 ggaacatact gttaagttta ctcacttgac ttcggtttat gtgtatgaga aagtcggcga 53940 taaacagcca atttacggtg tatttcgtga aattactgaa gatggcacaa cttactggaa 54000 ggaaatttat taatggctat taaatttgaa gttaataaat ggtatcaatt taaaaataaa 54060 caggctcaag aaaattttat taaagaccat actgataacg gaatctatgc acgacgttta 54120 ggtatggaac cttttaaaat tttagatgtg gactattacg ggcgtcctac taaaattgtg 54180 acatctactg gcacagttgg atatgcaaca catggtgata tccttgacga aaactttatc 54240 tggctttcta ctaacgaagc tgggttcttt gatgaagtgg aaaatccata ccaggcatct 54300 gaagaacaag agcaggaaga gcaagaacaa atagaagatt tcacagaatt cccagtcatg 54360 aaagttacta ttgaaaataa tgaacaggca tggtccttgt atcagatgtt gaaagcacac 54420 tttaaggaat aattatgcca atgtatgatt ataaatgcca atccgaagat tgcgggcatg 54480 aatatgaaaa aattaaaaag atttctgaac gagaaaatga tgtttgccct aaatgtcatc 54540 gtttgtctac tcgtcggcct tctgctccta agcatgtaaa tggtggtttt tacgacttac 54600 ttaaagggta attatgttta aaatcggtaa gaaatatcgt attcgcgaag gtgaagaaca 54660 gaaatatcta ctttctgcta gtaataggaa tagttctatt aatgccgtaa tatctacaag 54720 cgaatttatc gttgaagata tgaaaggtca taatgttaca atgattagta cagcatctgg 54780 aaatgatgga aaaattcttc atagttgtca gagtaatgtt ctaatttatg atgaagaatt 54840 tgacttcttc aaagaagttt ccgaagattt taattttgaa tgtactatca ctatgaaatc 54900 tggcgaccct ctttctttta cagttaaaga tgaaagaagt cgcttgagaa ttattagtct 54960 ccttcaagcc attaaatttg agtgaaatta tgaaatatgt taatcgttct atcgcggcat 55020 tagtattagc agtatcttta gtaggatgta ctgatgctga taatgcaacc aaagttttgt 55080 cttcaagtgg ttttactaat attgaaatca ctggatataa ttggttcggt tgctctgaaa 55140 atgatttcca gcatactgga tttcgtgcta ttggacctac cgggcagaaa gtagaaggaa 55200 ccgtatgttc tggtctattc ttcaaggatt cgactatccg ttttaaataa aaggccttcg 55260 ggcctttagc tttatgatta ccggagtata atattcccga aaccaaacga ggataagtga 55320 tgattaagaa tgaaattaaa attctgagcg atattgaaca tatcaaaaag cgtagcggca 55380 tgtacattgg ttcttctgct aatgaaatgc atgagcgctt tctgtttggt aaatgggaaa 55440 gtgttcagta tgtacctggt cttgttaagc ttattgatga aattatcgat aactcagtag 55500 atgaaggtat tcgtactaag tttaaattcg cgaataaaat taatgttact attaaaaaca 55560 atcaagtaac agttgaagat aacggtcgtg gtattccaca agcgatggtt aaaacaccta 55620 ctggtgaaga aattcctggt cctgttgctg cctggactat tccaaaagca ggtggtaact 55680 ttggtgatga taaagaacgc gtcactggcg gtatgaatgg tgttggttct agtttaacta 55740 acattttttc tgtgatgttt gttggtgaaa ctggcgacgg tcaaaataat attgtagttc 55800 gttgttcaaa tggcatggaa aataaatcat gggaaactat tcctggaaaa tggaaaggaa 55860 ctcgtgttac tttcattcct gattttatgt catttgaaac taatgagcta tctcaagttt 55920 atcttgacat tacactggat cgtctccaga cacttgcagt agtttatcct gatattcaat 55980 ttacctttaa cggtaaaaag gttcagggta attttaagaa atatgcacgc caatatgatg 56040 agcatgctat cgttcaagaa caggaaaact gttctattgc ggtcggtcgt tcacccgacg 56100 gttttcgtca attaacatac gttaataaca ttcatactaa gaatggtggc catcacattg 56160 actgtgttat ggatgatatt tgtgaagacc ttattccaca aatcaaacgt aaatttaaaa 56220 ttgacgtaac taaagcacgt gttaaagaat gtttgactat cgttatgttt gttcgtgata 56280 tgaaaaacat gcgatttgat tctcagacta aagagcgttt gacttctcca tttggtgaaa 56340 ttcgtagtca tattcagctt gatgctaaaa agatttcacg tgctatttta aataatgaag 56400 caattttaat gccaattatt gaagctgctt tggctcgtaa attggcggca gaaaaagcag 56460 cagaaactaa agcagctaaa aaggcttcta aagctaaggt tcataaacat attaaagcga 56520 atctttgtgg taaagatgct gacacgactc ttttcttgac tgagggtgat tctgctatcg 56580 gatatcttat tgatgttcgt gataaagaac ttcatggtgg ttatccatta cgtggtaaag 56640 ttctcaacag ttggggtatg tcatatgctg atatgcttaa aaataaagaa ctgtttgata 56700 tttgcgcaat cactggatta gttcttggtg aaaaggctga aaacttaaat tatcataata 56760 ttgccattat gactgacgct gaccatgatg gtctaggaag catttatcct tctctgctcg 56820 gattttttag taattggcca gaactgtttg aacaaggaag aattcgcttt gtcaaaactc 56880 ctgtaatcat cgctcaggtc ggtaaaaaac aagaatggtt ttatacagtc gctgaatatg 56940 agagtgccaa agatgctcta cctaaacata gcatccgtta tattaaagga cttggctctt 57000 tggaaaaatc tgaatatcgt gagatgattc aaaatccagt atatgatgtt gttaaacttc 57060 ctgagaactg gaaagagctt tttgaaatgc tcatgggaga taatgctgac cttcgtaaag 57120 aatggatgag ccaatagttt actttaccac aaggatgtgg tataattaat tgggcaaatg 57180 aggatattga aatgaaatca tataaagtaa atttagaact ttttgataaa gcagttcatc 57240 gagaatatag aatcattcaa cgctttttcg atatgggaga agccgaagag tttaaaaacc 57300 gctttaagga tattagagat aaaattcaat ccgacaccgc aactaaagat gaactactag 57360 aagttgctga agttattaaa cgcaatatga attaatgagg aaattatgat tatcaccact 57420 gaaaaagaaa caattcttgg taatggttct aaatcaaaag catttagcat cacagcgtct 57480 cctaaagtat ttaaaattct atcatctgat ttgtatacaa acaagattcg cgcagtagtt 57540 cgtgaattga ttactaatat gattgatgcc catgctctta atggaaatcc tgaaaaattt 57600 atcatacaag ttccaggacg attagatccg cgatttgttt gccgagattt tggtccaggt 57660 atgagcgatt ttgatattca gggtgatgat aattctcctg ggttgtataa ttcatacttt 57720 agttcatcta aggctgaatc taacgatttc attggtggat ttggtttagg ttctaaatct 57780 ccgtttagtt atactgatac gttcagtatt acttcgtacc ataaaggtga aattcgcggt 57840 tatgtagctt atatggatgg tgatggtcca cagattaaac ctacattcgt aaaagaaatg 57900 ggtccagacg ataaaactgg tattgaaatc gtagttccag ttgaagaaaa agactttaga 57960 aattttgctt atgaagtttc ttatatcatg cgaccattca aagatttggc tatcattaat 58020 ggtcttgacc gtgaaattga ttattttccg gattttgatg attattatgg cgtaaatcca 58080 gaaagatact ggccagatcg tggtggattg tatgctatct atggtggtat tgtttatcct 58140 attgatggtg ttattagaga ccgtaactgg ttaagcattc gcaatgaagt gaattatatt 58200 aagtttccaa tgggttcgtt ggatattgct ccatcgcgtg aagcgctttc attagatgat 58260 cgtactcgta aaaatattat tgagcgtgtt aaagaactca gtgagcaggc atttaatgaa 58320 gatgtaaaac gatttaaaga atctacatct cctcgtcata cataccgtga attgatgaag 58380 atgggatatt ctgctcgaga ttatatgatt agtaattcgg tcaaattcac gactaaaaat 58440 ctgtcatata agaagatgca gagcatgttt gaacctgaca gtaagttatg taatgcagga 58500 gttgtgtatg aagtaaatct tgaccctcga ctgaaacgca ttaagcaaag ccatgaaact 58560 tcagccgttg catcaagtta tcgtctgttt ggtattaatg caacaaaaat taatatcgtt 58620 attgataata ttaaaaatcg tgttaatatt gtccgtggat tagcccatgc gttagatgat 58680 aaagaattta ataacacttt aaatattcac cataatgaac gtcttctgtt tattaatcca 58740 gaagtagaat cgcaaattga tttacttcct gatattatag caatgtttga aagtgatgaa 58800 gttaacattc attatttgtc agaaattgaa gctttagtta aaagctatat tccgaaggta 58860 gttaaaagta aagctcctcg tcctaaagct gccacagcat ttaagtttga aattaaagat 58920 ggacggtggg aaaaagagga gttatttact ctcacatcag aagcagatga aattactggt 58980 tatgtagcat acatgcatcg ctctgacatt ttctctatgg atggtactac atctctttgt 59040 aatccatcta cgagtatttt gattcgtatg gctaatctta ttggcattaa tgaattttat 59100 gttattcgtc cgcttttaca gaaaaaggta aaagaactcg gtcagtgcca atgtattttt 59160 gaaactctgc gtgatttata tgtagatgct tttgatgatg tagattatga taagtatgta 59220 ggatattcaa gttcagctca acgatatatt gataaaatta ttaagtatcc tgaactagat 59280 tttatgatga agtacttcag tatagatgat gtttctgaag aatatactcg ccttggtaat 59340 atggttagtt cattgcgagg tgtatatttc aacggtggaa aagataccat cggtcatgat 59400 atttggacag taactaatct ctttgatgaa ttgtcacgta atgcttcaaa aaacagtgat 59460 aaaatggttg ctgagtttac caagaaattc cgtattgttt ctgacttcat cggatatcgc 59520 agctctttaa gtgatgatga agtttcccaa atcgctaaaa ctatgaaggc ccttgcggcc 59580 taataaggaa aattatgtac aatattaaat gcctgaccaa aaacgaacaa gctgaaattg 59640 ttaaattgta ttcaagcggt aattatactc agcaggaatt ggctgattgg caaggtgtat 59700 cggttgacac aatccgccgt gttttgaaaa atgccgaaga agctggtcgt tctaaagtta 59760 ctattagtgg tgacattaca gttaaagtta agagcgatgc agttattgct ccagttgcta 59820 aatctgacat tatttggaat gcatctaaaa aattcatttc aattactgtt gatggtgtaa 59880 cttataacgc aactcctaat actcattcaa acttccagga aattcttaat ctgcttgttg 59940 cagataaact ggaagaagca gcacaaaaaa ttaatgttcg ccgtgctgtt gaaaaatata 60000 tttccggcga tgttcgaatt gaaggtggaa gtttgttcta tcaaaatatc gaattgcgat 60060 ctggtttagt tgatcgtatt cttgattcga tggaaaaagg tgaaaatttt gaattttatt 60120 ttccgttctt ggaaaatctg ttggaaaacc caagccaaaa agcggtgtct cgactctttg 60180 atttcttggt agcaaatgat attgaaatta cagaagatgg ccacttctat gcttggaaag 60240 tagttcgcag taattacttc gactgccatt caaatacttt tgataacagt ccgggtaaag 60300 tagttaaaat gccacgtact cgcgtgaatg acgacgatac acaaacttgt tctcgtggtt 60360 tgcatgtgtg ttctaaatct tatattcgtc actttggtag ttcaactagt cgagtcgtaa 60420 aagttaaagt acaccctcgc gatgtagtat caattccaat tgattacaat gatgctaaaa 60480 tgcgtacctg ccaatacgaa gtagttgaag acgttactga acaattcaaa taagggcttc 60540 ggcccttaac tataaaggaa aattatgtta ggttatcaag cacgagtaaa agaagaatac 60600 gatcagctaa tgctcaaaat taatgcgttg agcaaatttt tagaaagcac aaagttttta 60660 acagttaatg aagttgaaca agaattactg ctttcacagt ttatctcaat gaaatcttat 60720 gctgactgct tagagaaaag aattgcgcaa ttcaaataat taaagggctt cggccctttt 60780 gttttaaggg aaattatgat ttattatatg aatatcagtg attccgatat aaaagagatt 60840 aatctacacg gaaatcatca cgctaacatc gtgtactgta acaaatttga gtttggtcat 60900 gaaaatttag gatttcttta ttgcaatgat gttatagtca tggataaaaa gaaactcgat 60960 gaacttgatc gtgaatcttt agatgaaaat gatcgaattt attatggtac tcttaaagta 61020 tataacgctt atctcagtgg taaaaaggaa agtctaaatg cagaaaacga atccgggttt 61080 acagagactg tttcagattc cgacatttac cctatcgaac aatgatttga ctggtgaaat 61140 gaagatcaaa attgctgata ctgcaagata ctctttaaaa caaaatccaa accaagataa 61200 agcagaagtc atcgaaagat gccgtatcgc tgtgtatgca gagttttttg tagcagattg 61260 gctgagcgga tatgttaaca aaggtcaaga ggatgttaat gacccgtata catacgcatg 61320 ggatgtattg gcacatccaa gatactgtgg gcttcgagta gaagtcaaga cacatcaaac 61380 tgactcacgc tggatttctg taacaacagg atgcagcgga gagtatccat acggttctgg 61440 aataaatcta gggcccattc tgaatcatca agtcgcagac tgtataatta tattcaacac 61500 taaagaaatt catccaggtg tcatccagta cactccgaag ttcattggtg acagagaaga 61560 ccttcgtaag gttgtaagaa aaagtaacta caacggatgg tatctttcca tttaaaaatt 61620 ttcacaaaac ggtttacata ccacaaggac tgtggtatta tacaactatc aactacggag 61680 gaacagaaaa tgaaatttaa gttcttttac gttaaacata aaattaccgg tgaatttatt 61740 gcatttacga cttcaactac agatgaagga gatattttta ctgcagtatt tttatcaaaa 61800 tgggaatcag atcaacctta cttatcatca cgtgaagatc ttcaacaatt agttaatgga 61860 gaatataatg attcatggtc atatttagtc catgattgtg ttaaaaaggc aataaaacaa 61920 aaacgcttgg aaatcgttga gattgaacta tgagttcatt atggtggtgt ttcgtttggt 61980 taattagtat tcctttaatt tgtttagcat ttacttttgt gatgaggtta ttatgaaaat 62040 tttgaattct gtacttattg cttgtgcgtg gtgggttgtg caagtttcag cggtagtagt 62100 tggtattcac atttattacg aatattttta aaaaagttgt ttacaagact gttcctctgt 62160 ggtactatac aactatcaac tacggaggaa cagaaaatga aaaagattgt taaagctata 62220 tggaatgtaa ttataatttt agttgttttg agtatattcc caattgtttt aatgattgat 62280 gtattaaacg cttactttgg atttatgtga ggaaaatatg aagcgtaaac gtagtgcttt 62340 tacatttatt gaatggtttt tcgataatat ttttccggct ttattcattt tcatgctgat 62400 tttcgcctta ggttcagttg tagttggaat ttatttaatg gcggttgtcg gaatggatat 62460 tcaccaaaac ggtttaaaat ctgtagttga aacaatttgg aatggtgtaa aatgatgaat 62520 ttgttgagtg gttggtttta tattcttatg ttttacattg gtgcaaattt cccgtattgg 62580 atgggatggt caacaactgc atttggattt tatactcctt gaggtgaatt atgaaaatct 62640 ttaaagatgt aaaagttggt gaaatttttt gcttggatag cggtgatcag ttaattcgca 62700 tttcacctct taagagtacc agcgagaaaa tgacagttaa cgctacttta gtaaataaca 62760 gtaatgaacg tttctgtatt gaaaatgata ctgaaactta taccgtagaa gagttctggg 62820 aattgagcgt cgactgcgac gattaattta tcggccgtgt gtattcatgc ggccttggag 62880 tagaaaataa tttagaggaa attaatatga aatacatgac ttctagtgat ttacgtgatg 62940 caggcgctac tattattggt acaatcaaga atggcgaatg gtttttggga actccgcata 63000 aagacatttt atctaaacct ggattttact ttttagtgag taaattcggt ggtccatttg 63060 gttgtccatg cgtatctgca cgattttatg taggtaatca gcgttctaag caaggattca 63120 gcgcagttct aagtcatatt cgtcaacgtc ggtctcagct tgcgcgtact attgcaaata 63180 acaatgttcc atacgcagta ttttatctgc ctgcttctaa gatgaaacct ctgacgactg 63240 gattcggaaa aggtcagtta gctttagcgt ttactcgtaa tcatcattct gagtatcaaa 63300 cgcttgaaga aatgaaccgt atgttggctg ataactttaa atttgttttg caggcatact 63360 aatgaaagct ctatttgtaa tgggttacct attagtttta gccttattca cattgactgg 63420 aatctttacg gctttaggtc tacctcttaa tgataatgtg gctgtcgtga tgtgtgtagg 63480 tggcggattt atctgttttg aacgtttatg caaaatgtgt ggtgtgtatg agtaatttcc 63540 ataacgaaca tgtgatgcag ttctatcgta acaatcttaa aaccaaaggc gtcttcggac 63600 gccagtgagg aaaatatgaa tatcgcaaaa ttattaggag ttatttcatt tatttgttgg 63660 atagtagcat gtgttttaac tatctgtatc gatgctagca gtgtgttttc acaagcttta 63720 gcccagggta tgtgtgcata tttaacattt gtgttgttat ctactaatga ttaagaaaat 63780 cttgggctat tcattagccc ttgctgcttt attggtagca ctatattatg gagtaatgtt 63840 cggattaatt caagtcgtgc ttttcatttc tgatgttatt atggcactac attcactagt 63900 atggtaaatt tatgcaactg aataatcgcg atttaaaaag tatcattgat aatgaagcat 63960 tggcttatgc tatgtacacg gttgaaaatc gtgctatccc aaatatgatt gatggattta 64020 agccagttca acgatttgtt attgctcgag ctcttgattt ggcacgagga aataaagata 64080 agtttcacaa actcgcttct attgcaggtg gtgtagcaga ccttggatat catcatggtg 64140 aaaactctgc acaagacgca ggtgctttga tggctaatac ttggaataat aactttcctc 64200 tgttagatgg tcaaggaaac tttggttctc gtaccgttca aaaagcagcg gcaagtcgtt 64260 atatttttgc tcgtgtaagt aaaaatttct ataacgtata taaagatact gaatatgctc 64320 cggtacatca agataaagaa cacattccgc ctgctttcta tttgcctatt attcctactg 64380 ttcttcttaa cggtgtttcc ggtattgcaa ctggttatgc aacttacatt cttcctcata 64440 gtgtttcttc tgtcaagaaa gctgtattac aagctcttca aggaaagaaa gtaactaaac 64500 cgaaggtaga attcccagaa tttcgcggtg aagtcgttga aattgacggt caatatgaaa 64560 ttcgtggaac atataagttt acttcacgaa ctcaaatgca tatcactgag attccatata 64620 agtatgatcg tgaaacttat gtgagtaaaa tcttagaccc gcttgaagat aaaggcttca 64680 ttacgtggga tgatgcttgt ggcgagcatg gttttggctt caagattaaa ttccgcaaag 64740 aatattcttt gagcgataat gaagaagaac gtcatgcaaa aattatgaaa gacttcgggt 64800 tgattgagcg tcgttctcag aatattacgg ttattaatga gaaaggaaaa ctacaagttt 64860 acgataacgt agttgattta attaaagact ttgttgaagt tcgtaaaact tatgtccaaa 64920 aacgaattga taataaaatt aaagaaactg agtcagcttt tcgtttagct tttgccaagg 64980 cacatttcat taagaaagta atttcaggtg aaattgttgt gcagggtaaa actcgcaaag 65040 aactgaccga agaactttct aaaattgata tgtattcttc ttatgttgat aaactagttg 65100 gaatgaatat ttttcatatg acttcagatg aagcaaagaa acttgctgaa gaagctaaag 65160 ccaaaaaaga agaaaacgaa tattggaaaa ctaccgatgt agttacagaa tacaccaaag 65220 atttagagga aatcaaatga gtccattcat aggtattaca agcgctgcat tagtatctgg 65280 tggcattttg ctggcgggtt taggcgttgt tccagccgta gcaggaggtc ttcttgcgtt 65340 cggaattcaa cgtgttatca tgacagttat cacagtcatg cagtaatctt aggggagcct 65400 cggctcccct tttttatttc aaaaattttt tcacaaaacg gtttacaacc aaagcattcc 65460 atggtactat acaactatca actactgata cagaattacg gagattagaa aatgtctaaa 65520 gtaacttaca tcatcaaagc ttctaacgat gttctgaatg aaaaaactgc cgcaatttta 65580 attaccattg ctaagaaaga tttcattaca gctgcagaag ttcgtgaggt gcatccagat 65640 ttaggtaacg cagtagttaa tagtaatatt ggggtattga ttaaaaaagg cctggtggag 65700 aaatctggtg atggattaat cattacaggc gaagctcagg atattatttc aaatgcggca 65760 actttatatg cacaggaaaa tgctcctgaa ctactgaaaa aacgagcaac tcgtaaagct 65820 cgtgagatta cttccgatat ggaagaagat aaagacttca tgttaaaact tttagatgaa 65880 aatggatttg ttcttaaaaa ggttgaaact tatcgcagta actatctcgc tattttagaa 65940 aaacgcactc atggaattcg taattttgaa attaacaaca atggaaatat gcgaattttt 66000 ggatacaaaa tgatggaaca tcatattcag aaatttactg acatcggtat gtcatgcaaa 66060 atcgctaaaa atggtaatgt gtatcttgac attaaacgct cagcagaaaa cattgaagct 66120 gtaatcactg tagcatctga actgtgagga ataaataatg aacaagttag aaattgttaa 66180 tgaacttcgt cgttgtgcag aacctactca agagggttgg gacatctggt accacggggc 66240 ttatcttgga actatcgtaa agattaagac tggtaaatac atgattattc gtgaaaataa 66300 agatgctcca gtaggtattc gcaataattt catgacagcg ataagttcct ttacggattc 66360 agcttacgaa atttaccttg ctgattataa agaattccag gaatctcaac cagttattcg 66420 ctcaattggt gttaacaaag ctcaacagaa aactttgtgg cagcgtatta aaggatggtt 66480 taaatgaacc catttattaa tcgtttaaaa atgctgaatg tgcctttatc tcgtgaaact 66540 ccagaaagtc ttgttgaaaa atttaaagca catggttata aatgcacaga agaagatatt 66600 cttgcagaag ttcctgaaat ctgttggcag accgcatact gggacgaaaa ccaaaagtat 66660 caacgacgaa ttgtttgcgc agcaaatcgt tttaaattaa aagatggacg aactcttgtt 66720 attccaggtg ctcgtcatta ttctaaagat atggcagaag ttttagatgt agttaaacct 66780 caattagtta ctcaacaagt ttgtgatgat gaccaagggt ttattgacca atatagtaat 66840 tattggaccc gtgaagaagc aatgattatt gcaacttacg ctggacaagt acgtatcgaa 66900 cgcggcggta gtgaaaaaga actttactct gaggaccttt actaatgaat attaaaaagt 66960 ttcaaattga tggaattacg aatcaaatcc aggcactgga atatgccaat aaaatgatgt 67020 caactaattg gggaatttat gccaatgaac cgggatttaa gttctgtgat atggaattca 67080 ctaaaaagct cgtaggaaaa gattatgtat gtccatttag ttctataata aatgacatgc 67140 taaaacctgc tttgcgcgat ctttatattg cgatgaacga agaaatgata aaagaactaa 67200 aacgtcaact gaaggtgatt caatttggcc agggaaatta attcaaaatc cgattatttt 67260 aattctctca acgataaaga taaaatctaa tacggcattt tattgttgaa atgggatata 67320 ttgatacgca tgatttaaga gaacatatat ttgaatgtgg tgtagctaaa aagttttcat 67380 tcacatgcaa atgtttaaga gaggtaattc agcactatga acaatttagt cgcaaagcat 67440 aactttaata aagcttctgt tcataaagat aagaagaaag cgtttaaaga atccaatcgc 67500 aaacagaaac ataaggggaa ggtctatgat tattaattct cagtctgtgg ttcaatacac 67560 aatcaaaatt gatattctag aaaagctata taagtttcta ccaaatttat accactcgat 67620 tgttaatgaa ttagttgaag aactacatct cgagaataat gatttcttgg ttggaactta 67680 taaagacctc tcaaaagcag gatattttta cgtaattcca gctccaggaa aaagtattga 67740 tgatgtatta aaaactataa tgatttatgt tcttgattat gaaattgaag attatttcga 67800 atgagtcata atcttgaaaa agtaatcgag cataatgtag ctcaggaacg taagtcgttc 67860 aaggaattcg tagaaaaaat ttttgaagaa aataccacag accagtttac aaatcaagcg 67920 tctgatgata ttataataaa gtcaactaat tgagtggtat agttaatgaa taaaaatatt 67980 gatacagttc gtgaaattat tactgttgcg tctattttga ttaaattttc ccgagaagat 68040 attgttgaga atcgtgctaa ttttattgca tttctgaatg agattggagt aacgcatgaa 68100 ggtagaaagt tgaatcagaa ttcattccgt aaaattgttt ctgaattaac tcaagaagat 68160 aagaaaaccc tcatcgacga attcaacgag ggttttgagg gtgtatatcg atatctagag 68220 atgtatacga acaaataatt atttagccct tcctaatatt ctggccgcct gagcacatat 68280 tgattcaagg cggtcattac ttatatgatc atttctatac cagtacatgg ttattgttcc 68340 agcatagata ttatccaaat tgaaatatgg acaactgtac atgtaattta tttcgggagt 68400 aggcttttta gtcggtaaaa aagcaaattt tgagttggaa taataatgac gtccatttaa 68460 atgaactgca tattcatcca tagttttatc aacaggatat cctccaagtg atttttcact 68520 tattgttgaa ggtaattttc cttcatatgc tataatatca acaaaatagt ttaagttttt 68580 agggcggaaa gaatacaccg cactaaagtc tgcctcagat gatatatgaa ctatctggag 68640 ttgttccagg gcgacagatt caaagcgtgc atttctttcc ttttcaataa tttcactgta 68700 tgtttcatac tttgattgct tatagtactc aaagaaacta tctcccctat accaaacaat 68760 cgccattata aacaaaagaa ttacgacagc tacccgggaa gcaagaactt tcccggtagc 68820 gttatctttg aacaagcgat ctagaacacc aaacagaata tcagagggcg aaaatgatat 68880 tctaggtgct gccatagacc ctccttttaa ggatatttat tcaaaattat actcttgcac 68940 catatattgt accaacttta gtccaagttg gagcattacc agtaacagct ttacctgctg 69000 cgccgccgtt atattctgat ccttgaccgc gaatattaca tttacctcct gcagctccgg 69060 catcaccacc attcccgcca ttatagatac cgttgacaga ccctttaccg ggtgcagaaa 69120 tagtaccagc ggtagcacct tgttccatat tagcagaacc accagcagcg ccaaatggac 69180 gaccgcctcc tccgccaaaa gtcaatttca tttgtgaaaa aggagagtag tatccgccgc 69240 cgccaccgcc accgcctcct gcgataacac cattgttgtt aatacgtaat ctagttccaa 69300 ttccgttatt tattgcagtt cctcctgctc caccggcaga gttatttcca ccattcccgc 69360 cgcgacccca aacatgcaca cctgaattga tattaagtgt cacatacgca ttagttaacg 69420 agctaggaaa atctaagcat ggaacaccag tagattgaga aacaatattt cctgtaatag 69480 taataacaac aggagtgctt ccttgggctt ttatccaatt tattagtgtg tctttattat 69540 aagaattgct agcaccaatg ctgtaatata cttctttaga ttgtccggcc attgagctca 69600 tccaaccagg aactgaaaca cgaactgctt tcattgcagc atccatccaa cgttgtccag 69660 tttcattcac ggcagattca ccaatccagc caggaacacc tacaattgcc attaaaaatc 69720 cttagagggc cgaagccctc ttttacataa gtttagattc aagttctttt acacgagaag 69780 aaagtgtttt cacagcttca accagaagag caatctgggc ttgagaagaa acagtgagta 69840 ttttattacc cttgctgtct tctgtttcat ggacagcttc tggtaaaacg tcttgcaacg 69900 tttgagctat aatacccgct tcggtttgaa caggttcacc gcctatatat tcagctttgt 69960 catagattaa accatcaagt tgctcaaccc tttcgagcgc atttttaata ggtttaaaat 70020 tagatttcaa gcgaacatca gaacgaatat aaacgttagg agcattgacg tttttctcgg 70080 aataaacttc acttgaggtg aatctaacaa ctttaacgtt gttcgcccac gcttctaaaa 70140 gcccatcgcc tacttgtcta aaaccagtat cactatcacc aattgcaatg gagttaccac 70200 cccaaccatt tgttgtatta ataccaaaag agttagtctg tgttataccc gaaagaacag 70260 cagagttaaa tgaacattgg ttactaccgc ccggtgaacg ctggaaataa accaaataat 70320 ttgattcatc ggcaatttca atacggttgc cacgtgtggc atctccagca tggaatatac 70380 tcatgtttct tggattagcg ccagacttat taaagtgaat ataaccgttg tttttgaggg 70440 caatatcgtt atagaactct gatatcccag aaacactcat cgagtttgaa atagttactt 70500 tacctgaagc gatattgata gaaaaaggtc tcaagctatt ataagaaccc gaggctttag 70560 ttttatcagt atcggtattt ttatctgtta ataatatgtg gaacgtagta ccatcattat 70620 aaaacagagc agctgcagag ccgctttcgt caccgttaga tttgtgacga tatcctatag 70680 tagtatttgc cacaaaagtt gcatttgtag aaatattaga actacccgcg tcaaaaacgc 70740 ctgagctatt attaagaagt ttaaaaacac gacctttaac aatatctaca ggaagctctg 70800 ctactggtga ttgcgtaggc ccatataatc catgaactac cacagaagca ttttctgaag 70860 tgctatattc tatggtaagt ctatttaaat aagtgccgca actaatataa acatcatact 70920 catctcctga ggtattaata tacgcaatat ctttaatagc atctgtttca cgttgccata 70980 aaacaccgtt aattccttta ggcgtattat taccagaacg taatacaatt tcattaattg 71040 ttgcttgttt gaaaatacca acgttaaaac cggaaccgcc tgttatttta aattggactg 71100 tagctacacc ttgaggcata gttactgttg ccaatttata ccaaccgctt acagaagaat 71160 agttaatcca gccggtagtt actttacggt ttaaagtcgt atctaagtta gaaaaattac 71220 cagaaccacc ggtaatatta ccagttctat caaatctgac tttgcctgct attaaatcgc 71280 gaggtgaaat aaaatcaccg tttttaataa attcccatcc aaaatctgct gtttgtggtc 71340 ctgaagaacc gttatctccg ccaccatgat aatgaaccct gaagttacca ttattaatta 71400 aagttcctaa tgaatagcaa ccattacctt ggacataacg ttgcttcaaa ataggaacat 71460 aagtgcttgt atcggttcta tctatattca tgtagaacgg agcacgaacg ttttcattat 71520 tttgtgaagc gaacgaacct gcaccagcag ggcgaacagc atcagtacat tctgcatcaa 71580 tgtacgcagc ctgacctaac tgcatacggt aattaggaga aatacgacta tgactagtaa 71640 caacaacaag tttattatcg ttgtctacag taatcatatt gccgcctctg ccgtcatcgc 71700 ctaacgtgac agaatgacgc atttgtacca taccattatt aaggctgata cttagtgggc 71760 ggagattact tattccacca ctttcaccgg catctttagg agtcgggata atatgtaatg 71820 atgcttctga acgacggaaa atagcaccat attcagcatt ccaaattctt aatgcatctg 71880 ctgttccacc aaatttagct tgtccattta cagataatcc attatttgca actaaactgg 71940 attcagtaac aatagaacca gatgatgtaa taccgccaga agttaataat gctccggcaa 72000 ctctaaattg aattggtccg acagtcgaac ctgttgctgg agctacacgc tgtgcataaa 72060 aataatatcc ctgcccgtca gccacttcaa aaactgtttc acgagcacga tcgccagatg 72120 cgttaaatga attaccccac gctttaatag atacataact tttgttctga cctgcgcgag 72180 taccaccagc ccacagtata gttttagtgc catctgctga tgtaggagcg tcaagttgaa 72240 taccgtcagg tctagcacca gtaagaacta atccgttatc aaatttaatt ctttgtattg 72300 aagatactgt tccattagca taaaactgta cgttattaga gccagttacc aatttcaaaa 72360 taccagggtt aatttccata ccttcctgtg tattaatgtt ggtttggcct ttaccgcgga 72420 aataatggga aaatttaccg ccagaattat atccaatgtg ggtttgacca tcacctgcat 72480 tgtttgtatc agcttgggtt tgaaccgata aaagcgcggc gttggttcca ggcattgtcc 72540 aggttccgcc ttggtcttct gagcgtccaa ataatgcttt acgagtacta cggaaactat 72600 ccggggtaat agatgcaacg gaattccaat tacctactaa atcataaacg ccttgcttaa 72660 tatattttaa accggttgca gcatcaccca aagcaatata tctttctccc attggagtag 72720 gagagccata attgttagta ttttcatcag tggtaattgt gccaatagca agcgaacgac 72780 gtaataccat tgcgccatca ttacgaagac ccattaattt agtagtaagc gagtttccgg 72840 tccaccaaga aatttcatca ttcttaccgg tttgagctga agcgatttca tggtaaatag 72900 ttccacctga tttagctcga actttacgaa gatagtttat accattttct tcgcctgttc 72960 caggataaac atattgaact aatgattgcg aatcatattg tccgaatgct tttgtatcta 73020 aaataatatt tttagtatta atttgaggtg aagtaatatt tccaatagct gcaatatttc 73080 cagatgtagt tatactacct cctgatactg ctaaacctcc tgtatcaaca acaaatccac 73140 cgtcacgtcg aaacgtccaa tatttcgaat cgccagattc attaatataa tgaatcttca 73200 acgaaccttc agaaactaaa gtacctaatg aaaaagttcc atctttaaag cgctgcttaa 73260 ttataggatt atattcagat gtggaaagag ctgagctcaa atctacaaaa attggggctt 73320 ttgcagcatg ttgaccagcc caagccccac ttccagtagt acgataatta aactcaatat 73380 atccaccgct tgctacaatt tgcggaccaa ttaaattaaa ttttccagtc tgtgtataat 73440 caccggtttg agtgatattt ccactgatat taccgccagc agaaatgctt aaatcaatga 73500 tatttcctga atcatcttta gtaaaaattg ttctatcttt taagtttata gccaattcac 73560 cttcggctaa tactgaagca gcaggacgtg cacctgcggt tttgcttctt ttaaattgta 73620 tttgttttaa agtagccata agtcctctta ataatagccg aaatcttgaa cagaatcctt 73680 aattacgatt tggtcaaatc gcggaacgtg tgaaggttga gacgcaggat tctgcgagaa 73740 aaagtttggt gcggttaagt ttcctgtcat agtgtcacca gatcgtaaca ctctagagtt 73800 tgcatttgcc gtaacagtat ttattgctcc atcaacatag tcctttctag taaggtcatt 73860 tgctgctata ggagcggccg catcacttct tacttgacca gtcgccgaca caacaccttt 73920 tgtgctaata tcaccattac gtgtgttaat taccacagtt cgagctgctg aaccctgtga 73980 tgatttaaaa ccaataccat accatgaaac aatatcaata tttgctgtag caaaagtagc 74040 tgcatcgcca ttacctggat aaataccgca cgggtttgta ttgtttacag catatggtac 74100 ttgaacacca gcattaaatg ttacctttga cgcaaaagta ccaccattag ctttagaaac 74160 gaaatcgtta tcaacagctt gtggtttatt atattctgaa tatattttaa atgatttata 74220 gagtacatcg tcaccggctg gattcaatgg aaaatttcct tgatgccaaa tgacagagcc 74280 tccagttgtt gaacctactt ttaaatcagc cattgtatgc ccctttattt taatagtatt 74340 tataaagaaa aagggaaccc gaaggctccc tcaatttata cttctctaaa ttcttgtcca 74400 aacactttac cagttttaga cgatgcttgc gtaggaagta ccattatatc cggaggtgaa 74460 gcagattcac agatataatt aacacgaata ccattgacgc caaattcagc aggtttcgaa 74520 atacctccat ttcttgacac ttcagaaaag cttaaacttc tcatgccacc ttgaccagct 74580 tgtgctgttc tttgcgcata tatcgtaaat ccgacagcgt tttctggaac aactacataa 74640 tcttccttta attcccaaga cccagcttgc ccggtaaact cagcttgtgt cgaggaaata 74700 tatccattcg atgcattata aaaacggata gatatatttg tagttccaag cgcaagtaaa 74760 tcggcatcag catataactg tgctttaaga taaagaacat caccagggat taaattatag 74820 tcagataatt tacttatcgc agctgaagtt ggtagacgtg caatttcgtt attagttcca 74880 ccaaccgctg acataaattg ctcaacgctt tcatacgttc ttcttggaaa cccggtagct 74940 ccaacgtctt ctaaactgtc aaatacaaca tctaaaatag tttgatagtc atctgtgctt 75000 tttctattac ttagtttaac atgctctaat gcaatagctc ttttagaaga agtgtaaaaa 75060 gcagcgtatg atacgtcaaa tcttgacaat atagaatcag acggaaaagc agacgttcct 75120 gcggctctta gccaagatac tacttcagga ggaaaattaa cctttccgct agttaatata 75180 gcaacaagtc tgttattcgt caaagaattc atgaaactaa caaaagcagc cgatgtagta 75240 tcatttgaag ccgaaaaagc atatgactta ctatcaacta atgctccggt ggaagggtca 75300 aaaactctta aatgaagtcc tgtgttaaat gtttgacttc caacaggatt atcctgaaat 75360 ttaacgtatg gtcctgccgt agaaagcggg caagaacccg ctatacttat tttatatctt 75420 actgaattac tttccgataa aaatggcgtt tggacatatc cttgtccaaa ctctgccata 75480 aatttttcca taatacctct tattcaaccc attcaaattt aaccgtttta ttcactgggt 75540 caggaataat gcgaacatta ccaattcgta agaaatcacg aatagtaaga ttccccattg 75600 tagcattatc agatggtaaa gcaccaatat cagatggttg tggagggtta cctccatcaa 75660 atacctgaac aaaacttgac caagagtttt tggttttctg ccatgtacgc gtccaacgag 75720 tggtgcgtgc ttctggagtc gttggataag taatccaatc ttggtaaagc gaatcaagtg 75780 tgttaccaaa ctgagtcaat gtaccaggag atttaacttc ttcgccacgt tctaagtacg 75840 gaagcccagt cacttcatta gttttttcaa ccattttaaa ataacctgga aactggttat 75900 aagtagctga atcattaata tcgattgacc agaatcctac agtatcagat gttggcgcac 75960 gggtatataa gtcagatgtt ttagtgccct gagaacgaat tctcgagtta acagttaaac 76020 cacctgaatt tatagtagca cctttggcaa tgattaagct ttcaccaatc gttacttgac 76080 cagatacatt attaatagct aaaggacgta atccattaaa tccgccagtc tgatcacccg 76140 atgcagtaag cataaaatag gtgttagcag catcattgcg aataaagaat ccatagtcgc 76200 catttattgc tctaaaagca tttgcagact tgctgatgaa ttcaccctgc gcggtcaccg 76260 aacgaccgaa tgttgcaacg ccattcacat tcattaaccc ggaagcatta atatttatcg 76320 gcattacagt accattaatg ctaaacgcta tattaccatc tttattgcgt tgagaataaa 76380 agtgattaga cgtttcatca ccaacttcaa atacagtaga acgtgttgta tctgaaccgc 76440 caccaaactg atttccccaa actctgatag tcatcgtctg cactgggtta gttccagttt 76500 gaggtccttt ctcaaaaatc agacgagttg ccgttccagt attagaaata gttaatgtgc 76560 tgtttgccga aactgatccg ccaaacgtag cagtactaga tgatacaaga ggggcactca 76620 gattcgtttg ttgggttaag gttagtgaac cattaaccgt ctgtgcaata tccctacgaa 76680 tgaattgaga tgaatctaga ccatctaata aatttgtatc agcagctttt gcttttaacg 76740 gcaaataatt tgctaataca cggtttaatt catatggcga tactgcatag ctatttttct 76800 cgtatagctc taagtcctgc gtagaaccag ctgtatcatt accaacgaat gtaattgaac 76860 cagatgaagt tttaacaaag cctcttatcg cagtagtagc tgcccaagta ggttcactct 76920 gtacaatcca ttttaaattt tttggagata cagcagtatt tgctgacgtt ccagttacag 76980 tttcagactg agttgcaact ttaataacac cttcctgcga ttcggtagat ttagtaccta 77040 aaagcttttt aggagttatt aaaacattat ccaatgttcc tgcagcagct tcaacctgtg 77100 tagctacacg aagtgtacca cgttgtgtct catttgcttc aagaatatta agggtataat 77160 ggtcccagag agttcctgat tcaactaatc cagatagagc aacaacagaa gtacgatcag 77220 tactattaaa tctagtttta atttttaatg gtgtagagat acgagtatcg tcgacgcctg 77280 cgtcgaattc aacttgtgta gcaatttcag ctataccact taaactttca gttgctttac 77340 ggtcatttaa agttttagga gtgactgcac gagtataatc agttcctgta ttaacttcac 77400 tttgtgtagc aatttcaatt aaaccaattc ttccatcagt tgatgtcttt ttatgtaacg 77460 tttctggcgt tacaaccgca tttgcccatc cttcttgact ctgcccagca attacttcac 77520 tttcaactgc taaaattact gcaccttgtt gtgttggagt agctttatac tgatccagag 77580 ctttaggcga aacaactaaa ttattagtgt ttttattata aacgtttgta ccatttaatt 77640 cacgactaga agctggagta gctcctgcgg tagatacaaa ggttacaata cctgataatg 77700 attcagaacc ctgacgagct tgaagctttt taggagtgat gattgtagta tcatcagtac 77760 ctgcattagt ttcctgttgc gtagcaattt cggcaacacc tctacgagtt tctgtagcag 77820 ttctttcatt cagcttttta ggagtgatga taatatcatc agcaaaagag aatgtagtat 77880 cctggtttac ttgagcagta gttgctattc ttgcgatacc tctgcgggtt tctgtagcag 77940 tacgattagc taatgtttct ggagtaattg ctaattcttt ttgaggtgaa ttttctaaat 78000 cagcatttgc ttgagcctgt gtagctaaag caattacacc taatcttgct ctagtagaat 78060 catttaaaga atctactctt tcaactgttg gaacgttttg ctgtacaacc cagtattttc 78120 catcagaatc ttctatataa gcaagttgta aaactggaac ataattagtt tcaccgttaa 78180 aaactaattc ttggacagtt acccatttag tttcaggcgg atattctgaa cgttttggga 78240 attgcaataa ttgaactgaa gaagcaattt tatcttctgg agcagcttta attttaactg 78300 tttgtccttt tctcatgtaa ttcatggaaa ttttaacagt atcaccaaca gaaatattag 78360 ttggaagctg aagctcaatc gtttgagttg ttccattatt cgcaccaaat accataactt 78420 cttcgtttgg acgaatgttt gaattagtcg ttatgatgcg taaacgtgct ttactgtccc 78480 cgtcaaacaa tctccataat ttctcattat catcaaacat caagaaaccg tcaatcgatg 78540 tacggccttc aatggaatga gttccatctt cttgtactga tgtcgtttca tcatatgtcg 78600 taacaattgt atgataaaga gggtttaatt tatccaaatc gacgaaatta ataatatcgc 78660 cgtgattagc aaatctcgga agtttaatat ttatcggtgc agcagaagta aatctacgta 78720 cgataaagtc gttggattgc gcttgataag tattcgctgg tgttacaact acagcttctc 78780 tactataatc agcaatatac atttgccaca gacgattact aaaaattaaa actatctgtg 78840 attttggatg agtcattagt actgatcgta cttgctgacc tctaaaattt acaatgcttt 78900 gtactggagc tacaattaaa acttgattaa cgccaggttt tcctccaata tcttggagaa 78960 cgatagtatc accgtcaatt ggtgaagacg gtaaagtaaa tgtaatatca ttaccagctg 79020 cagtattaac agaaattgct tcaccggatt ttaactgata agaaccagat gaaactgtag 79080 tccagttagc atcagtacgt aatgctctcc aacgtgcgct attaaaagct cctgctggtt 79140 ttgaaatatc atttatagca gcccaaaagc ggttatcata aatgattaca aaatctttta 79200 aatatccacg agttggatca tattgttgaa ctgtattttc ttgaattaag taatcaacgt 79260 taacaccgtc agttcctacg gtacgattag ctaaagctac gttgattatt ttatcgccac 79320 ctgcgtccag accatcttct gctctgaact ttcttttaat ctcggccatt ctcccgggct 79380 cctattgtgt tttcaataat aagtatttat acttgtttac tttaagattc ggatggtata 79440 taatagaaat ctcactaatt gaacgaggtt catatggatt tagaaatgat gctggatgaa 79500 gattacaaag aaggaatctg cttaattgac tttagtcaaa ttgcactttc aactgcttta 79560 gtaaactttc cagataaaga aaaaattaat ttatcaatgg ttcgtcattt gatattgaac 79620 tcaattaagt ttaatgtcaa aaaagcaaaa gctcttggat acactaaaat tgtactgtgt 79680 attgataacg cgaaatctgg atactggcgt cgtgatttcg cttattatta taagaaaaac 79740 cgtggaaaag cacgagaaga atctacttgg gactgggaag gttattttga atccagtcat 79800 aaagttatag atgaattgaa agcttatatg ccatacatcg ttatggatat tgataagtat 79860 gaagcagatg accatattgc tgttcttgtt aaaaagttct ctttagaagg acataagatt 79920 ttaatcattt catcggatgg tgactttact caacttcata aatatccaaa tgttaagcaa 79980 tggtcaccga tgcacaagaa atgggttaaa attaaaagcg gttctgctga aattgactgt 80040 atgactaaaa tccttaaagg tgacaaaaag gataacgttg cttcagttaa agtacgatct 80100 gacttttggt ttaccagagt tgaaggtgaa cgaactcctt caatgaaaac ctctattgtt 80160 gaagctattg ctaatgaccg cgagcaagct aaggtgcttc tcactgaatc tgaatataat 80220 cgttataaag aaaatttagt tctaattgat tttgattata ttcctgataa tattgcttca 80280 aacattgtga attactataa ttcatataaa ttaccaccgc gtggcaaaat ttattcatat 80340 tttgtaaaag cgggtctttc taaattaact aatagcatta atgaattttg aggtgaataa 80400 tggctaaaaa agaaatggtt gaatttgatg aagctatcca tggcgaagac ttggctaaat 80460 ttattaaaga agcatctgat cataaactga aaatttctgg ttataatgaa ttgattaaag 80520 atattcgaat tcgtgccaaa gatgaacttg gcgttgatgg taagatgttt aatcgtctgt 80580 tagctttgta tcataaagat aaccgtgatg tgtttgaagc tgaaactgaa gaggtagttg 80640 aactttatga cacagttttc tctaaatgat attcgtccgg tcgatgagac cggtctttca 80700 gaaaaagaac tttcaattaa gaaagaaaag gatgaaattg caaagcttct tgaccgccaa 80760 gaaaatggat ttattattga aaaaatggta gaagagtttg gaatgagtta tcttgaagca 80820 actactgcgt ttttggaaga aaattcaatt cctgaaactc aatttgctaa atttattcct 80880 tcaggtataa ttgaaaaaat tcagtcagaa gctatcgacg aaaatctttt acgtccttct 80940 gttgttcgtt gtgaaaaaac taatacatta gattttctac tatgattaaa ctccgcatgc 81000 ctgctggtgg tgaaagatac attgatggta aatcagttta taaattatac ttaatgataa 81060 aacagcatat gaatggaaag tatgatgtaa ttaagtataa ttggtgcatg cgggtgtctg 81120 atgccgctta tcaaaagcga agggataagt attttttcca gaagttatca gaaaaatata 81180 aattaaagga acttgcttta atctttataa gcaatctggt tgctaaccaa gatgcttgga 81240 ttggtgacat ctctgacgct gatgcacttg tgttttatcg tgaatatatc ggacgcttaa 81300 agcaaattaa atttaagttt gaagaagata ttcgcaacat ttattatttt agtaaaaaag 81360 ttgaagtttc tgcttttaaa gaaatctttg agtataatcc aaaagtccaa tcaagttata 81420 tttttaaact tcttcagtcg aatataattt cttttgaaac attcattttg cttgattcgt 81480 ttttaaatat aattgataaa catgatgaac aaaccgataa tttggtttgg aataattatt 81540 ctataaagtt aaaggcttat agaaaaattt taaatattga ttcacagaaa gctaaaagtg 81600 tttttattga aactgtgaaa tcttgtaagt attaattgct tattataaat aaattataat 81660 tatctcattg accagctatg aggtcataca tcgtcatagc accaactgtt aaattaaatt 81720 aaaaaggaaa taaaaatgtt taaacgtaaa tctactgctg aactcgctgc gcaaatggct 81780 aaactggctg gaaataaagg tggtttttct tctgaagata aaggcgagtg gaaactgaaa 81840 ctcgataatg cgggtaacgg tcaagcagta attcgttttc ttccgtctaa aaatgatgaa 81900 caagcaccat tcgcaattct tgtaaatcac ggtttcaaga aaaatggtaa atggtatatt 81960 gaaaattgtt catctaccca cggtgattac gattcttgcc cagtatgtca gtacatcagt 82020 aaaaatgatc tgtacaatac cgataataaa gaatacggtc ttgttaaacg taaaacttct 82080 tactgggcta atattcttgt tgtaaaagac ccggctgctc cagaaaatga aggtaaagta 82140 tttaaatatc gtttcggtaa aaaaatctgg gataaaatca atgcaatgat tgcagttgat 82200 gttgaaatgg gtgaaactcc ggttgatgta acttgtccgt gggaaggtgc taactttgta 82260 ttgaaagtta aacaagtttc tggttttagt aactacgacg aatctaaatt cttgaatcaa 82320 tctgcgattc caaacattga tgatgaatct ttccagaaag aactgttcga acaaatggtt 82380 gatctttctg aaatgacttc taaagataaa ttcaaatcgt ttgaagaact gaatactaaa 82440 ttcagtcaag ttatgggaac tgctgttatg ggtggtgccg cggcaactgc cgctaagaaa 82500 gctgataaag ttgctgatga tttggatgca ttcaatgttg atgacttcaa tacaaaaact 82560 gaagatgatt ttatgagctc aagctctggc agttcatcta gtgctgatga cacggacctg 82620 gatgaccttt tgaatgacct ttaataaatt atattactaa ttaattgggg accctagagg 82680 tccccttttt tatttcaaaa ttttttcaca aaacggttta caagcataaa gctttatggt 82740 actatacaac tatcggcaat actgctgatg attaaagagg aaaataatat ggctaaagtt 82800 gatattgaca tcgttgattt tgaatatatt gaagaaatta ttcgtaatcg ttatcctgaa 82860 cttagtatca caagcgtgca agattctaag ttttggagta ttcaaatcgt tattgaaggt 82920 cctcttgaag acctcacccg ctttatggct aatgagtatt gtgacggcat ggattctgaa 82980 gacgcagaat tttacatggg attgattgaa caataattat caaggggcta ttacagcccc 83040 gttaaaatga ggaaaacgta atgtataatg gcaaaaagta tgagcttgtt ccaagactta 83100 ttgatacatt tattagttat cgcccacgtt ctaattcatc aatagttaaa attattcaag 83160 aaaatggcgg atggtttgaa gttaaagaat ctttctttgc tgacggattt aaagtaataa 83220 aacacattga atgcgcaaat ggaaagcatt tttactttaa cgtctgcgaa gatgaatttc 83280 attgttttcg tgagtataaa gaaccgactt ccgaagaaga tgaagtcgaa gacaaggttt 83340 ctggcgtaac aaaaattcac tgcattgtag acgaaaataa cgtcgacgaa atcattgaac 83400 ttttgcgaaa aactttcaaa aagtagttta cagtagggta gtagtgtgat actattaccc 83460 tatcaaaact aatggagaaa agaaaatgtt cgcaccttat attatggcag cagttatgtt 83520 agtttgctta taccttttga ttaaagcttg ctaaggagaa taaaatgaga ttacaacgcc 83580 agagcatcaa agattcagaa gttaaaggta aatggtattt taatatcatc ggtaaagatt 83640 ctgaacttgt tgaaaaagct gaacatcttt tacgtgacat ggggtgggaa gatgaatgtg 83700 atggatgccc tctttatgag gatggagaaa gcgcaggatt ctggatttac cattctgacg 83760 ttgaacagtt taaagctgat tggaaaattg tgaaaaagtc tgtctgagga aatatagtga 83820 aaacactaaa tttgcttaag gttggggaag acatagatat tgctgatatc tacaaaaatc 83880 taacttatta tgataattta tttgtcgttc aaaccttaaa tggtgattac tatacggttt 83940 ctttagtgcg aaataaattt tacttttgca tttataatta tcatggccaa cgctctagca 84000 attatattga accatctgac atattttata ttgaggaatt aaaatgatta aattggtatt 84060 tgcttattct ccaactaaaa cggtcgaagg ctttaatgaa ttagcattcg gcttaggtga 84120 tggtttacca tggggacgag ttaaaaagga ccttcagaat tttaaagctc gtactgaagg 84180 tacaattatg attatgggtg ctaaaacgtt ccagtcattg cctacattac ttcctggtcg 84240 tagacatatt gtagtgtgtg accttgcgcg tgattatcct gaaactaaag acggcgattt 84300 agcacatttc tatattactt gggagcaata tatagattac atttctggcg acgaaattca 84360 agtatcaatt tctggttcgc cattcgagac tattcttgac caaaattcta atgttagcgt 84420 aattggtgga cccgctctgt tatatgctgc attaccttat gcagatgaag tagtcgtttc 84480 tcgcatcgtt aaaaagcatc gtgttaattc aacagttcaa ttagatgcaa gttttcttga 84540 tgatataagc aagcgtgaaa tggttgaaac tcattggtat aaaatagatg aagtaacaac 84600 ccttacggaa tcagtatata aatgaaataa cactggctga aaatatgaat tttaattatt 84660 accctattct attagaaaaa gacgcgaaac aaccaaaatg gcagggtcct cagtttatta 84720 aaggcgtcta tcaattagta gttcctaaag acaaaattta tagcagttgt ttcactgaat 84780 ccgcttgtag tatttttggt aatagttctc cgtattggaa ttttgatata aaacttgaca 84840 gaaatatcga tatttggttg aaagctatgg atatcggcaa tatcacgttt gatgagaata 84900 attatcatat tattggtcgc ttttccaagc gtggtaaaga attatatttt acgcctgaaa 84960 ttgaaagaaa atttgatgct aaaccgtatt aattattagc ttcataaaat gtattataat 85020 ttatataagg tactaaatga aacaatacca agatttaatt aaagacattt ttgaaaatgg 85080 ctatgaaacc gatgaccgaa cgggcacagg aacaattgct ttgttcggta ctaaattacg 85140 ctgggattta actaaaggtt ttcctgcagt aacaactaag aagctcgcct ggaatgcatg 85200 tatttctgag ttactatggt ttctgtcagg aagtactaac gtaaatgatt tacgtcaacg 85260 tactcatgga tctttaattg caggaaaaac aatttgggat gaaaactacg aaaaccaggc 85320 caaagactta ggttatcata gcggtgaact tggtccaatt tacggaaaac agtggcgtga 85380 ttttggtggt gtagaccaaa ttgtagaagt tgttaatcgt attaaaacat ttcccaatga 85440 tagacgtcaa attgtgtcgg catggaatcc agctgaactc aagcagatgg cattacctcc 85500 ttgtcatatg ttctatcagt ttaatgtgcg taacggatat ctcgatctcc aatggtatca 85560 acgttcggta gatgttttcc tcggtcttcc atttaacatc gcgagttatg ctgctctttt 85620 acatattgtt gctgacatgt gtgaactagt tccgggcgat ttggtattct cgggtggtaa 85680 tacacacatt tattctaatc atattgagca atgcaaagag atattaaata gaactcctat 85740 ggatctatgc aaactcagta ttaattggcc taatggattc agtcaatgcg atactgaaac 85800 tcagatgtat tggttaactg aatatatgac agcacgggat ttcgttctca aagattatca 85860 gtcccacggt gtcctgaaag gaaagatggc ggtgtaattt taatttaatt gagaggatat 85920 atgattttac gatttaagga tacttctggt gtcgttcttt ttacacttcc taatccaagc 85980 gagttagaag ttccaggacc agaacaacct attatcattt atggtaaaaa atattatact 86040 cataaaatga ctcgtgagta ttttgataat aaaatttcta cagttaaaac ttcttctgat 86100 tgttattatg atattactgt tttaacggaa aaacaatatg acgaattatt ccagcgtggg 86160 ccgtctatgc cgagtagtga ataaatataa atccgacttt gatgttaata ttcaccgtgg 86220 tacattttgg ggaaattacg tcggtaaaga tgctggcagc cgggaggctg ccattgaatt 86280 attcaaaaaa gattttatac gtcgaattaa atccggagaa ataactaaag cacatttaga 86340 gcctttacgt ggaatgaggc taggatgcac atgtaaacca aagccgtgtc atggtgatat 86400 aatagctcat atagttaacc gattgtttaa agacgatttt caagttgagg acttatgcaa 86460 ttaattaatg ttatcaaaag tagtggtgtt tctcagagct ttgacccgca aaaaattatt 86520 aaagttttat cttgggcagc tgaaggaaca tctgtagatc cttatgaatt atatgaaaat 86580 attaaatcat atctccgtga tggaatgacc actgatgaca ttcaaaccat tgtaattaag 86640 gctgctgcga attctatttc agttgaagag cctgattatc aatatgtagc tgcacggtgt 86700 ttaatgttcg ctcttcgtaa gcatgtttac gggcagtatg agccccgttc atttattgac 86760 catatttctt actgtgtaaa tgaaggtaaa tatgaccctg aattgttgtc aaaatattct 86820 gctgaagaaa ttacattttt agaatcaaaa attaagcatg agcgggatat ggaatttact 86880 tattccgggg cgatgcaatt aaaagaaaaa tatctagtta aagataaaac cactggtcaa 86940 atttatgaaa ctccacagtt tgcatttatg actattggaa tggcattgca tcaagatgaa 87000 cctgttgata gattaaaaca cgttattcgt ttttatgaag cagtatctac tcgacagatt 87060 tcattgccaa ctcctattat ggctggctgt cgtactccga ctcgacagtt tagttcatgc 87120 gttgttattg aggcaggtga ttcgctgaag tctatcaata aggcttccgc ttcaattgtt 87180 gaatatatct ccaaacgcgc tggaattggt attaacgttg gtatgattcg tgccgaaggt 87240 tctaagattg gcacaggtga agtacgccat actggtgtta ttcctttctg gaaacatttt 87300 cagactgctg ttaaatcatg ctcacagggt ggaattcgtg gcggcgctgc tactgcttat 87360 tatcctattt ggcatttgga agttgaaaat cttctcgttt tgaaaaataa caaaggtgta 87420 gaagaaaacc gtatccgtca tatggattat ggcgttcaac tgaatgacct aatgatggaa 87480 cgattcggaa agaatgatta cattactttg ttcagtccgc atgaaatggg tggcgaactt 87540 tattattctt attttaaaga ccaagaccgt ttccgtgaat tgtacgaagc cgcagaaaaa 87600 gaccctaata ttcgtaaaaa gcgtattaaa gcccgtgaac tgtttgaatt gctcatgact 87660 gaacgctcag gaacagcaag gatttatgta cagttcatag ataatacgaa taactatact 87720 ccgtttattc gtgaaaaggc acctattcgt cagagtaact tgtgttgtga aattgctatt 87780 ccaacaaatg atgtgaatag tcctgatgct gaaattggat tgtgtacgtt atctgcattt 87840 gtgttagata actttgactg gcaagaccaa gataaaatta atgaattggc agaagtccaa 87900 gttcgtgctc ttgataatct tttggactac caaggatatc cagttcctga agcagaaaaa 87960 gctaaaaagc gtcgaaacct tggtgtaggt gttactaact atgcagcttg gctggcaagt 88020 aattttgctt cttatgaaga tgctaacgat ttaacacatg aactatttga gagattacag 88080 tatggactca ttaaagcatc cattaagctc gccaaagaaa aaggaccttg cgaatattat 88140 tcagacactc gttggtctcg aggcgaatta cctatcgact ggtacaataa aaagattgac 88200 caaatcgcag ctccaaaata cgtttgtgac tggtcgtcgc tgcgggaaga ccttaagctc 88260 tttggcatcc gtaatagcac attatcagca cttatgccat gtgagtcatc ttcccaagtt 88320 tctaacagta caaacggtat cgagcctcca cgtggaccag tctctgttaa agaatcaaaa 88380 gagggctcct ttaatcaagt cgtgcccaat attgagcata acatagacct gtatgattat 88440 acgtggaaat tagctaagaa aggtaataaa ccttatctta cgcaagtagc tattatgctg 88500 aaatgggtat gtcaatcagc ttcagcgaat acatattatg acccgcagat ttttccaaaa 88560 ggaaaggttc caatgtcaat aatgattgat gaccttttgt atttttggta ttttggcgga 88620 aaaaatttct attatcataa tacccgtgat ggttctggta ctgatgatta tgaaatagaa 88680 actccaaaag ctgaagattg ttcatcctgt aaattatgat ataatttgac tcacggacga 88740 gtcactatct attaactaag cggaaaattt atgagcacag tttttaatac aaatccagtt 88800 gatgttttaa aagaacctat gttcttcggt tcaggtcttg gtattgcgcg ttatgatatt 88860 caacgtcata aagtttttga agatttaacc gaaaagcaat tgtcattttt ctggcgtcct 88920 gaagaagtaa acttgatgat ggatgctgca caatttaaca agcttcctca gtatcagcaa 88980 gatattttta ctaataacct gaagtatcaa tcacttctag atagcattca gggtcgtgca 89040 ccttctgctg tacttatgtc attaatttca gacccaagcc ttgatacatg ggttgctaca 89100 tggactttta gtgaaactat tcacagccgt tcatatactc atattatgcg gaatctttat 89160 actgatccgt caaaagtatt tgatgagatt gtattagata aagctataat gaaacgtgct 89220 gaatctattg gtcgttatta tgatgatgtt attaagaaaa ctagatattg gcagaatgct 89280 aaagctgata ttgagtacca aaaagaaatt aacgcagacg aagatgatat taaagatgcc 89340 atagagcatg aaatgtattg gaaacgtgaa ctaatgcgct ctctttacct ctgcctgcac 89400 gtaatcaacg cactggaagc tattcgtttt tacgtatctt tcgcatgtac ctttaacttc 89460 cataaaaaca tggaaatcat ggaaggtaat gccaagatta tgaagttcat tgcacgtgat 89520 gaacagcttc accttaaagg cacccaatat attattcgtc aacttcaact tggcactgat 89580 ggtgatgaat gggttaaaat cgcccaagag tgtgaacaag aagcagttga tattttcatg 89640 gaagttaacc gtcaagaaaa agactgggca gttcatttat ttaaagatgg cgatgttcct 89700 ggattaaata caaatagcat gtggagcttt attgattact taactgtatc tcgtatgaaa 89760 cagtgcggtc ttccatgccc aattaccgat gctccggtta aacatccgta tccttggatt 89820 cgtgaatatc ttaattctga taatgttcaa tccgcgccgc aagaagtaga actgtcatct 89880 tatcttgtag cacagattga taatgatgtt gatgataaag ttatgatgag ttttaaaaaa 89940 tatttttaag gagtgggccg caaggcccat tttattatga aagaaatcgc aacagaatat 90000 tcatttatta aatatactga gctagaatta gacgacaatg gaagtataaa acaattatct 90060 attccaaaca agtataacgt aatttatgct attgctataa atgatgagct tgtttatatt 90120 ggaaaaacta aaaatttacg taaaagaata aactattata gaactgctat taatcgtaaa 90180 gacaaaacgt ctgattctac taaatctgca ttaattcatg ctgcgctaaa ggaaggaagc 90240 aaagttgaat tttacgcccg ccaatgtttt aatctttcta tgacaaatga gttaggtaca 90300 atgacaatcg caacgattga cctagaggag ccactattca ttaaactgtt taacccgcct 90360 tggaatattc aacacaagaa aaaatgatgc ttccacatgg agtgtggtac tatattcaaa 90420 acacaaaaga ggatacacaa tgcaagaact ttttaacaat ttaatggaac tatgtaagga 90480 ttcacagcgt aagttttttt actcagatga tgtaagtgca tctggaagaa cttacagaat 90540 tttctcatat aattatgcat cttattctga ttggttactt ccagacgcat tagaatgtcg 90600 tggaattatg tttgaaatgg atggagaaaa accggtaaga attgcttctc gtcctatgga 90660 aaagtttttt aacttgaatg aaaatccatt cacgatgaat atcgatttaa atgatgttga 90720 ttacattcta acaaaagaag acgggtcttt ggtatcaact tatttagacg gtgatgaaat 90780 tctgttcaaa tcaaagggtt caatcaaatc tgaacaggct ttaatggcta atgggatttt 90840 gatgaatatt aatcatcacc agttgcgcga cagacttaaa aaattagccg aagatggatt 90900 tactgctaac tttgaattcg ttgctccaac gaatagaatc gttcttgctt atcaagagat 90960 gaaaatcatt ttattgaata ttcgtgaaaa tgaaacaggc gaatacattt catatgatga 91020 catttataaa gatgctgttc ttcgtccata tttagttgaa cgatacgaaa tcgatagtcc 91080 taaatgggtt gaagaagcta aaaatgcaga aaacatcgaa ggctatgttg ccgtgatgaa 91140 agatggttct cattttaaaa ttaagtctga ctggtatgtg tctcttcata gtacaaaaag 91200 ttcattagat aatccagaaa aattgtttaa gactattatt gatggtgcat cagatgatct 91260 taaagcaatg tacgctgatg atgaatactc atatagaaaa attgaagcat ttgagacgac 91320 ttatctgaag tacttagacc gagctctgtt tttagttctt gactgtcaca ataagcattg 91380 cggcaaagat agaaagactt atgcgatgga agcgcaaggc gttgctaaag gtgctggaat 91440 ggaacatctg ttcggtatca tcatgagcct ctaccaaggg tatgatagtc aagagaaggt 91500 tatgtgtgaa atcgaacaga attttttgaa aaattataaa aaatttatcc cagaaggata 91560 ctaagctgtt tacaagtccc tcgtgttgtg ttatagtaat cttactgata taacatgagg 91620 actttatgat ggatttgcag cttattacta ctgagatggt cgttgaagca tacggtgata 91680 ctacagatgg gatttctgta tttaaaggaa atcgtcgagt tggatatatc accgatctta 91740 agaaagattt agctaaacaa gtcaagcgga aaacgaccat taaagaatat cgaaatcgtc 91800 gtcttgagca agctcgtgat atgcttcctg atgcggttga ggagatgaaa gtctttttag 91860 aaaatcagct tgcgaaatat gattgtgatg tgttcattaa tcagactcaa cctaatgttc 91920 atattaacaa ctgtaaatgc tatatcatcg ttaatccttt aactggaaaa catcgtcttg 91980 gaattagtaa tccaaatcgt agcgcatcgg atatggcaga agatgttgag gcatgcttta 92040 aaatttctaa atctcctgct gaacatcata ttttaattaa cggtctttct caagacgata 92100 ttatagaggt tattaaaact ttatgcaatt aagtaatacg acagcaggct tgctattaat 92160 tgtaattgca ttgggcggaa cttctttaat tttaaaaaat aaaattgaaa gattagaaac 92220 gtctgttgta gaaattacaa aaacggccaa tgaaaacgct ttagcattaa ataatttgcg 92280 aattcagtat aattatattg atgcgatgaa taataaaaat cgtgaggcaa ttgctgctat 92340 tgagcgtgaa aatgaaaaac tgcgcaaaga cgcaaagaag gcggatgtgg tggctcataa 92400 gccaggattg gttgaaaaac aaatcaacaa ctccttcaac aagtttgcag aagacatcca 92460 ggacctttct aaatgattaa actatcagca gtaatattat ctattggtct tctagttggt 92520 tgttcgacaa agcctctaga agtaaagaaa gaaacagttc atcctaattg gccagtacaa 92580 ataaagtcat atgatgaagc taaactatcc tggcaagtta aagttattga tggtaaagct 92640 tgggtcggta tgccattcga agattcccag gaatttcgta tttggcttaa tgatgtaaaa 92700 cgatatgtac atgatcagaa aactatgata tgttattatc gccaagagtt aaaagaggat 92760 aaatgcaaat gatttcatgg catcaatttg aacatctcaa aggattgatt tatgaatccg 92820 agatggctgc aatgatttac ggacgccaga ttcagcggtt agaatcttta cctccaacta 92880 atgatgtttt attagctcaa tcacgtgcta atcttaaaaa tgaataccaa aataagtggg 92940 gtaaagcatc taaagacttg catgattata ttcaatcatt ggttgagaaa taataaatga 93000 aaactctgtt agaacgttat attgaatgtt cagaccgtta cattgatgca tgcaatggtg 93060 cagtatatat ggatttggac cgtggggtag tattaaatga tgaagaccct gcgaaagctt 93120 tagatgatgc aggtaaagca ttacgaaaag aagcaaaagc ccgtgggctt gatatgtatc 93180 agcttaaaaa tcacatgata aaatttattt catctaatgt tcagagcaaa tcggtgaatc 93240 aatcaacagc tgaattatat aaagggcggc gtgagcataa tattcgtatt cttgaagttt 93300 tcttaggaat taaatgatga aaaagattat tttgactgtt ggatgtcctg gttcaggtaa 93360 gagcacttgg gctcgtgaat ttattgctaa aaatcctgga ttttataata tcaatcgtga 93420 tgattatcgt caatccatca tgggtcatga agaacgtgac gagtataagt ataccaaaaa 93480 gaaagaaggt atcgtaactg gtatgcaatt tgatacagct aaaaatattc tgtacggtgg 93540 cgattctgtt aagggagtaa tcatttcaga tactaacctg aatcccgaac gtcgcctggc 93600 atgggaaact tttgccaaag aatacggctg gaaagttgaa tataaagtgt ttgatgttcc 93660 ttggactgaa ttggttaaac gtaactcaaa acgtggaact aaagcagtac caattgatat 93720 tttacgctca atgtacaaaa gcatgcgaga gtatctcggt cttccggtat ataaagggac 93780 tcctggtaaa ccaaaagcag ttatttttga tgttgatggc acattagcaa aaatgaatgg 93840 tcgcggtcct tatgaccttg aaaaatgtga tactgatgtt atcaatccta tgattgttga 93900 actagctaag atgtacgata agcaaggata ttacattgta gtcgtttcag gccgtgaaag 93960 tggaaccgaa gaagacccaa cgaaatatta tcgtatgacc cgtaaatggg ttgaggatat 94020 tgctggcgtt ccattagtta tgcagtgtca acgcgaacaa ggtgataccc gtaaagatga 94080 tgtagtcaaa gaagaaattt tctggaaaca catcgcaccg cattttgatg tgaaattagc 94140 tattgatgac cgaactcaag tagttgaaat gtggcgtcgt atcggtgttg aatgctggca 94200 agttgcttca ggagattttt aatggcgtgg catcatgaaa cttggtctat tgttattgta 94260 aatagtggtt tagttggtac tagtaatggg caattttgtg tattcactag tgaaatcaga 94320 gcttgggagg aatgtcttaa attaagagaa aagaatcctg atgttgaact aacagtaaag 94380 aaaactaaac tacctttacc atggaaaact tatgaataac atagaaaaga tttatcgact 94440 ttgtgataga attgaaaaag aaaagaaata tctattttgt ttatggccta ttgttgatgg 94500 aagagtaggc ttagatgttc ttgattatga aacagaagac aaagtagatg gttcaacttt 94560 tgataatgcg ttggatgtta ttgattggct tgaagaaaat tatgtgaggt aaatatgttt 94620 ccaacttact ctaaaatcgt agaagtagtg tttagccaaa ttatcgctaa taatatgttt 94680 gaaaagcttg ataacgcagc tgagcttcga attcatgctc aagtgactca tgtattgaac 94740 actttgcttc cagaccaggt ggattctatt gccattacgc tgtatccaag ttcagcacat 94800 atcattgtcg tatttggtct tgatgctgag ctagtcatca aaggcgatat tcgttttgaa 94860 tcgcagacat cagaattcaa agcaatttaa tagtttactt tacggtagag ttgtgatatt 94920 atagctctac caaaataaat gaggaaattg aaatgagcga atggtttgaa gaagataagg 94980 tttatcgctt taaagctgga tataaagata tttttaatga aacttgtggg gctaataaac 95040 gaattgctca gtttattggg gaaaattcat ttaaagtaaa aatagatcct gcgaaaaatg 95100 ttattagcat taaacgcgaa attgacgatt gctggtataa agctgttgat gtaatgggcg 95160 aatcctataa agttagcccg ttattttcaa ttgcttatat gttagaatat tcttttttcg 95220 aagaagttca aaaagatgat tctgtcagta aatttgaaat taaaactgat aaagaaatta 95280 agtggaaagt agtaggtatt actggttgta tgttttatat ctatgctcaa actgatacga 95340 aggaagaagc taaaaagaaa gctctagaat atcttgaaga gtatgaagaa ggcccggtaa 95400 tgattaccca agatgccgaa ttagtttctg tcaaattagt taaaaacgtt gaaagtaagg 95460 agctaggatc aacatgctaa gcgaaaaacc aattattgtt aaagaattcc aagaaaaagt 95520 taaactattt gctcaggaat tggtaaataa ggtttctgaa cgatttccag aaacatcggt 95580 tcgtgttatt accgaaactc ctcgttcagt attagtaatt gtgaatccag gtgatggtga 95640 tcaaataaca catctcaaat tggattttga tggattagtt gaagcacaaa gggtgtatgg 95700 cgtactatga tgaatttaac tgatataatt gataattgtc ttgaaaatga tactggcgat 95760 catagagcgc ttgactctga aacagcacag ttcattagaa taactttaat gaatgatact 95820 ctagtgaata gtattcatcc ttctgtgtat gatgctatta ttgtgacgaa gtatccggtt 95880 gagcttcaca aaaagatggc tggtgcaatt tttattgata agaaaaaccg ctttaaagat 95940 gggcagaata taattagttc tgttattaaa agtataacta aacttcgtca cgaaatttat 96000 cgtggtgaaa ctgctaaatc tgcttatctg gtgattatga aatgaaagcg agtacagtac 96060 ttcaaattgc atatttagta tcacaagaat caaaatgttg ctcctggaag gtaggagcag 96120 taattgaaaa gaatggacgt attatttcta ccggatataa tggttcacct gccgggggtg 96180 tgaactgctg cgattatgct gctgagcaag gttggttgct gaataagcct aaacatacta 96240 tcattcaagg tcataagcct gaatgtgtat cgtttggttc aactgatcgt tttgtcttgg 96300 cgaaagaaca ccgtagcgct cactctgaat ggtcgtctaa aaatgaaatt catgctgaac 96360 taaatgcgat tttgtttgct gcacgaaacg gttcttcaat tgaaggtgct actatgtatg 96420 taacactttc tccttgtccg gattgtgcaa aagccatagc tcaatctgga attaaaaaac 96480 tggtttattg cgaaacatac gataagaata aacctggctg ggatgatatt ctgcgaaatg 96540 caggtattga agtgtttaat gttcctaaga aaaacttgaa taagttaaat tgggaaaata 96600 tcaacgaatt ctgtggtgaa taatgaaatt tcgtttggta cagctcacag caattagttc 96660 ttattctaac gagaacattt catttgctgt agagtataag aagtattttt tctctaaatg 96720 gaaacagtat tataagtcag aatgggtttg tattgatagg ccgtatagtt ggaaatctga 96780 tttagaaaaa tgccaaaagt tgctttccac tcttaaagaa cgtggaacaa cccatattaa 96840 aactgtaata ggtaaataaa tgaaattgac aactgaacag aaagtagcaa ttcgtgaaat 96900 tttgaaaact aaattgtcca tgggtatttc aaacgtggtt tttgaaaagt ctgatggtac 96960 tattcgtacc atgaaaggca ctcgtgatgc agactttatg ccaaccatgc aaactggtaa 97020 attgactgaa tctgctcgga aagaatctac tgatatgatt ccggtatttg atgttgaact 97080 cggtgcatgg cgaggttttt ctattgacaa attgatttcc gttaatggca tgaaagttga 97140 gcatttgctt caatttattg gtaaataaat gctttaagaa ttatttgtta ttattaattc 97200 atctgttaac aaaaaggaaa aacgatgtct gaagtacaac agctaccaat tcgtgctgtc 97260 ggtgaatatg ttattttagt ttctgaacct gcgcaagccg gtgatgaaga agttacagaa 97320 tcaggactta ttattggtaa acgtgtccaa ggtgaagttc ctgaactgtg tgtagttcac 97380 tctgttggtc ctgatgttcc tgaaggtttc tgcgaagttg gtgatttgac ttctcttcca 97440 gttggtcaaa ttcgaaacgt tcctcatcct tttgtagctc tgggtcttaa gcagccaaaa 97500 gaaattaagc aaaaattcgt tacctgtcac tataaagcta ttccgtgtct ttataagtga 97560 tataaataat aatatgaatt gggtgtcgga ataataagtt aaccgaacaa ttctacgtgg 97620 tagtctacaa ctgagagatc tgtcgaaaga agatgaaatt cagaagaacg tgactaccga 97680 gttttaatct ctaacgagaa tttttaaatg attaaacaat tacaacacgc tcttgaactg 97740 caacgaaacg catggaataa tggtcacgaa aactatggcg catctattga tgttgaagcc 97800 gaagctcttg aaatcctgcg ttatttcaaa catctgaatc ctgctcaaac tgcattagct 97860 gctgagcttc aggaaaaaga tgaacttaaa tatgctaagc ctctggcttc tgccgcacga 97920 aaagcagttc gtcactttgt ggtaacactg aagtaattta ttggagagtc actgccttag 97980 tgtgaagtta aaacgaggag ccgtcgaact ttgttttacg gtgtacctct tgaatgttga 98040 cgaggtttat ggttatcctg tcggtaaata ttcaaaacct aagtacccct tgagggcttt 98100 cgcaggcaat gccaataagt cctgcatttt catttaaaag agaatttata atggcaaaac 98160 aagctaaagc aaagaaagca gttgaaaaga aagttggtga ttctaaacgc gctggttaca 98220 agcgtgggtc gaactctcgt atcaatcaaa ctgttgagaa gatcatgcgc cgagcacgtg 98280 cggttcttcg agatgatgct tctcgttttg gtaagcagaa agcataagtt aaggactcct 98340 tcgggagtcc ttttttattt tccaaagatt gcacaaagtt gtttacagta tggctccttt 98400 gtgatagtat tatcttacac aaacaaagga gaataaaatg aaaatcaatc ttaactcata 98460 catcaaaggt aaagatcacg acggctataa agctatcgaa actaaagaac ttcagtggca 98520 tttatataaa gaacaatttg aattcgtaga ttgtatgact cctgaaggtc cttctgatga 98580 tttctcttgg aaaataatgt taactaactt ttttactgga gatacatacg aattaaaaac 98640 tttgattctt ggtaaaattc gctgtgagac ttatgaagat aaagaaactg gttactctga 98700 agatgttacg tggtatcaaa atggtcgtat taccgctgac ctcttgattg aaaaaatgaa 98760 agctaaaggt gaattaaatt tagataattg gattaaagtt gcataaaata gtttactttg 98820 gtacaggata tgatattata tacctgtacc gcaattaaac atctcggaga ataaaaatga 98880 attacatcaa ctttgaacgt aaatatgttt ctaacggtat tgcaggttct attgatacta 98940 tctgcctttg gaaacatcaa aatggatcag tatgcgaaat tgaacagtat atgactccta 99000 actacgttta tatgcgattt gaaaatggca tcacggtttc aatcacgaaa gaaggttctt 99060 cttttaaaat cgcattggat gatgattttc gtcaacgcga tttagggact catccttgct 99120 ggaatggcgt taatcgcaag cttctggtta aaacttggat tcgtcatatt ctgagtaaca 99180 gagctaaacc tgagcatctt gaagcaatct ttgatgtagt tcttaacgaa tttgatattt 99240 aaataaaatg aggggcttcg gccccttact gaggaaaata ctatgtttat gactacttat 99300 tttgataccc gcaaaaattt ctgtgaagtg gttttctcta aggcacctaa agaccttcct 99360 gctcatttgc agcctactag tgaatcgatt aaaaactatg ttaatgtagt ctgcccttta 99420 gagttccgta ctgtaaatgg ccgcgatact ttagctatca ctaaacttaa tcgtgaaatt 99480 gacattgacc cttcaattgc gcgtgaaatt aatatttctg atattggcgg cggtaatgtt 99540 aaatcacacg gttttcagat gagattttaa taaaatgttt aagaaattaa tccagaaatt 99600 actgggcacg gaaatggtcg aagttactta tcgtgtgact gatgtctctc cgctcactga 99660 agaccattta gagccatata ttatgacaat taaaatgctt aaacatgatg gcggattatc 99720 tattgaagac cgtttaccaa gttatggtca ttgggctgat attgaaatta taagtattaa 99780 agatgtctga gttagagatt agaagcaatt ttagatggcc atcatgtgca ttaagtaatt 99840 tcgcccaatg gccttttgtt atggacggta tccaatttgg aggtctcgaa ggattcctcc 99900 aaggatgcaa ggtgaaaaat gttgaacaac agcgtcgtat atttgggtta tccgggcttg 99960 ccgcccaaca agctggacga gcttatgcta gagctcagga ccgtggcacg ttgttctggc 100020 ttggaactcc gttttcaaga tattccgaag catggaaaga attatacaca aatgcatatt 100080 ttgaagcagc gttccaaaac aagggctttc gcgatgcatt acaagcctcg aaaggaaaag 100140 ttttgaagca cagcatggct agtggtctaa caaaagatga tacaatacta accgaagctg 100200 aatttattga tgtgttaaac ctattaagag actctctatg aagccaacta ttttgactga 100260 tattgatgga gtatgtttaa gctggcaatc aggccttcct tattttgctc agaaatataa 100320 tcttccgtta gaacatattt taaaaatgat ccaagatgag aaatttattt ctccaggtaa 100380 actctttaat tgtgatgaag aacttggtgt caagttaatt gaaaaataca atcgttcaga 100440 ttttattcgt tatttgtctc cgtataaaga tgccctgtgt gtaattaaca aattaaaaga 100500 agattataat tttgtagctg ttacagcatt gggtgattct attgacgctc tgttgaatcg 100560 tcaatttaat ttgaatgctc tttttcctgg tgccttctca gaagtaatga tgtgtggtca 100620 tgattcttca aaagaagagt tattcaaaaa ggcaaaagag aaatataacg taatttgtta 100680 tattgacgat cttgctcacc actgcgatca tgcgagtgaa atattaaatg ttcctgttta 100740 ttggatggct cgaggagagc gtgataatat tccaaaaact gcgcagcgag tttatacatg 100800 gaatgatgta gaaaataagc ttttttcgcc gaaggaaaat aaagaaagtt ttgatagtga 100860 aaaagctata aaagatttaa ttgagaagat ggttaaaaac gattcttttc cttggaacac 100920 tacctggaga acgcctggat ttaatcctta taatcatcta tatcatccat atcagacaca 100980 tccgtttcag acatggaact atattaaacc tggtggcata gagtatttgt ataatagacc 101040 tacttgtggt gataatattt tccaaggagc attctaatgt ttgttgttca cactatttat 101100 gaaaatgaag gtaatactac acgtgattac ggtcacgtaa atcaattttt tagatgcaat 101160 ccagaattcc gagctcaaaa agacgaacga atttttaaaa aatgtgtaga gcaaggtttc 101220 atttatatca agcactggat gcaaggaaat aaagttagaa ctacatatca taggtcttta 101280 actgagctta atgatgaatt gatttataat agagctgtaa atcaaactct gaaggatgaa 101340 caatgattct taaaattctg aatgaaatag catctattgg atcaactaag cagaagcaag 101400 caattcttga aaagaataaa gataatgaat tgcttaaacg agtatatcgt ctaacttatt 101460 ctcgtgggtt acagtattat atcaagaaat ggcctaaacc tggtattgct acccagagtt 101520 ttggaatgct gactattacc gatatgcttg acttcattga attcacgtta gctactcgga 101580 aattgaccgg aaatgctgca attgaggaat tgactggata tatcaccgat ggtaaaaagg 101640 atgatgttga agttttgcgt cgagttatga tgcgagacct tgaatgcggt gcttcagtat 101700 ctattgcaaa caaagtttgg ccaggtttaa ttcctgaaca acctcaaatg ctggcaagtt 101760 cttatgatga aaaaggcatt agtaagaata tcaagtttcc agcttttgcg cagttaaaag 101820 cagatggagc tcggtgtttt gctgaagtcc gaggtgatga attagatgat gttcgtcttt 101880 tatcacgagc tggtaatgaa tatctaggat tagatcttct taaggaagaa ttaatcaaaa 101940 tgactgctga agcccgccag attcatccag aaggtgtgtt aattgatggc gaattggtat 102000 accatgagca agttaaaaag gaaccagaag gcctagattt tctttttgat gcttatcctg 102060 aaattagtaa agctaaagaa ttcgccgaag tagcggaatc tcgtactact tctaatggaa 102120 tcgccaataa atccttaaaa ggaaccattt ctgaaaaaga agctcaatgc atgaagtttc 102180 aggtctggga ttatgtcccg ttggtagaaa tatacggtct tccggcgttt cgtttaaaat 102240 atgatgtacg tttttctaaa ctagaacaaa tgacatcagg atatgataaa gtaattttaa 102300 ttgaaaacca ggtagtaaat aacctagatg aagctaaggt aatttataaa aagtatattg 102360 accaaggtct tgaaggtatt attctcaaaa ataccgatgg attgtgggaa aatgctcgtt 102420 caaaaaatct ctataaattt aaagaagtaa ttgatgttga tttaaaaatt gtaggaattt 102480 atcctcaccg taaagaccct actaaagcag gtggatttat tcttgaatca gagtgtggaa 102540 aaattaaagt aaatgctggt tcaggcttaa aagataaagc cggtgtaaaa tcacatgaac 102600 ttgaccgtac tcgcattatg gaaaaccaaa attattatat tggaaaaatt ctagagtgcg 102660 aatgcaacgg ttggctaaaa tctgatggcc gcactgatta cgttaaatta tttcttccga 102720 ttgcgattcg tttacgtgaa gataaaacta aagctaatac attcgaggat gtatttggtg 102780 attttcatga ggtaactggt ctatgaaagc ttacttagaa acaattgtcg ttgctcaaaa 102840 agaaggtgga gatgtttcta cttctgtatc acaaatcgtt ctcgaatttg tagatgcata 102900 tgcttataat aaatttacag aaacatttga tgcctacgaa aaaggtccaa agtttgaaat 102960 atatcgtact ctcttaccac tagattatta aaggccttcg ggcctttaat tttataaata 103020 gaataaacac tagagaggat atgatggaac tcattacaga attatttgac gaagatacta 103080 ctcttccgat tacaaactta aatccaaaga agaaaatacc acaaattttt tcagttcatg 103140 ttgatgacgc aattgaacaa ccaggctttc gtttatgtac ctatacatct ggaggtgata 103200 ctaatcgcga tttaaaaatg ggcgataaaa tgatgcatat tgttcctttt acattaactg 103260 ctaaaggttc aattgctaaa ttaaaaggtc tcggtccaag cccaattaat tacatcaatt 103320 cagtttttac tgttgcgatg cagacgatgc gtcagtataa aattgatgcc tgtatgcttc 103380 gtattcttaa gtctaaaact gctggtcaag ctcgacaaat tcaagttatt gctgatagac 103440 ttatccgcag tcgttcaggt ggcagatacg tccttcttaa gaaactctgg gattacgata 103500 aaaagtatgc atatattctt atacatcgca aaaatgtatc actagaagac attccaggag 103560 ttccggaaat tagcaccgag ctctttacta aagttgaatc gaaggtcggt gatgtttata 103620 tcaataaaga tactggagct caagtaacta aaaatgaggc aattgctgca tctattgcgc 103680 aagaaaatga taaacgttct gaccaagctg taatcgttaa agttaaaatt tcccgtagag 103740 caattgcaca aagtcagtca ttagaatctt ctagatttga aagcgaatta ttccagaagt 103800 atgaatctac cgcagctaat ttcaataaac ctgctaccgc tcctttaatt cccgaagcag 103860 aagaaatgaa acttggaatt aattcattag cttctaaaac taaggcagca aaaattattg 103920 ccgaaggaac cacagatgaa cttcattatg actataaatt cttttcaaaa agtgaggtta 103980 atgaagtttc tgaaaaaatt aaagacgtaa tttttaacgc gatcaaaaat gaaccgacta 104040 cttcaataaa atgtttagag aagtatgcag cagctattaa taaattcttt gaagaatata 104100 aagataattg gcttgataaa cataataaaa cccgtaaagg acagccagat gaagtctggg 104160 aagaaatgac taaaaattcc tggaacgcag caaaaactaa attcctcaaa agaatgattt 104220 atagtttttc tggaattggt gcaggtccaa tgattgatat tactattgcc cgtgatggtt 104280 ctaaatatac tccatcacaa aagcgcggca ttagagagta ttgcggttca ggatatactg 104340 acatcaataa tcttctttta ggacgctaca atccagaacg atatgatgta atgagtgaaa 104400 aagaaattga atctgctata aataacttag attcagcttt tgaaaatggt gaccgtatac 104460 cagaaggcat tacagtttat cgtgctcaaa gtatgactgc tcctatatac gaagcactag 104520 ttaaaaataa agtgttctat ttcagaaatt ttgtatctac ttctttaact cctatcattt 104580 ttggacgttt tggaattaca catgctggta ttggtctttt agaaccagaa gctcgtaatg 104640 aactgacagt tgataaaaat gaagaaggaa taactattaa tccaaacgaa ataagagcgt 104700 ataaagaaaa tcctgaatac gttaaagttc aaataggatg ggcaattgat ggagctcata 104760 aagttaatgt tgtatatcca ggaagtctcg gaatagcaac agaagctgaa gttattctac 104820 cacgcggatt gatggtcaaa gttaataaaa taactgatgc ttctaataat gacggaacaa 104880 catctaataa tacaaaactc attcaagctg aagttatgac tacggaagaa ctcaccgaat 104940 cagtaattta tgacggtgat cgtctaatgg aaactggtga agtagttgca atgacaggtg 105000 atattgaaat agaagacaga gttgactttg catcatttgt ttcatcaaat gttaaacaaa 105060 aagtagaatc atctcttgga attattgcgt cttgcataga tattacaaac atgccttaca 105120 agttcgttca aggataaatc atggaactta ttacagaatt atttgacggc gcttcggcgc 105180 cggttgttaa cttaaatcct aagcataaaa tacctcaaat ttttgctatt caagccggtg 105240 aagaaagcgt gcttcctgga tttagatttt gtacatacac ctctggcggt gatacaaata 105300 aaaacgttaa gccaggcgat aaaatgatgc atatcgtaat gataggtgtc aatgagaaac 105360 tatcattagt taaacttaaa aacttgggtg gaaatccaat tggtgtcatt aatgcggttt 105420 ttgatactgc tcttcaaaca atgaaacagt ataaaattga tgcatgccta ttccgcgtac 105480 taaaaagtaa aacaaatggc gcagctcgtc aaatgcaagt tattgctgac cgtttagtac 105540 gtactaaagg agcaggtcga tatgttcttt taaaggaaat ctgggattat gataaaaagt 105600 atgcatatat tatggtttac cgtaaaaatg ccaatttaaa agacattcca ggtgtacctc 105660 ctatttcaac tgagttattc acaaaagttg aatcaaaggt tggtgatgtt tatgtagatg 105720 ttaaaacagg taatgctgtt cctaaagctg ttgctgttgc tgcttctatt gctttagaaa 105780 atgataagcg cacggatcaa gctgttattc agaaaactaa aattagtcgt cgattggcag 105840 cacaagctca atattctact gtcgatgctt cgcttcaggg tgatagcttc gctgccaaga 105900 aatatcaaga gtttgaatct aaagttccgg tatataaagc agaaggacca atgaactctg 105960 gtgttattca gattggttca aacttcggca aaggagctat cggtggtatg agaagtgctt 106020 ctcgttttaa atctaacgat tatgaactag aaagcttccg aaatcatatt gcattggccc 106080 atgcacgttt acgtgatccg tctatcagac tgcagagcga tataacacat caaggttctc 106140 aagaatattt aaagaataaa gaattttttg attataaaac cgataaaatt ttgagcgatc 106200 tcgctgacat taatatttct aatagctttg atgttattaa gaaaattatc aatgatttag 106260 ttaaaggttc taaagccaca ccagacgaaa aggtagctat tattcaattt gtcatgaatg 106320 gcatttataa attgattaat gaatctgctg ctcaggcata tgaatacgca agcaccgaag 106380 taactccaaa aggactgact caggctgagt ctgatgtaat tgaagattat tgtgcagatt 106440 catatgttga aatgaactcg ttcctcttgg gtaaaccaga ttctacccgt gaagaatata 106500 tggaacgagc tattaaacac atcgagacgc tggattctgc ctttgccaaa ggttcagttc 106560 ttcctccagg aactacgctt tatcgtggac aagaagttac ttttaaaact ttacgccata 106620 atattgaaaa taaaatgttc tatttcaaga acttcgtatc aacatcgctt aaaccaaata 106680 tcttcggtga gcatggtaaa aactatatgg ctctagatga ttccggcgca gtattttctg 106740 gtgaaggaga aggttctgtt gatgctgaag atttaatgca tatgggcagt cattctgctt 106800 atgcgaatga agatgctgaa actagcgtag gtatggtaat aaaaggagct gagcgaatta 106860 aagttatcgt tccaggtcat ttatcgggat ttccatcaga agctgaagtt attctaccgc 106920 gtggaatttt gttgaagatt aataaagtaa gcacgtacat gatgaaagaa actgcttata 106980 acaagtatct aatcgaaggc acaatcgttc ctccttctga acaattagaa gaatcggtat 107040 atgatggcga tcatctaatg gaaactggtg aagttcgtcc aatggctggg tttaatcaat 107100 tccttgtaga agaatcaaaa gaagaggaaa acgaagtttc tcaaatcttg gcttctttgg 107160 ttaacatcaa cggaatgtct aaaaagttca agatgtagtt tacaagtccc tcgtgttgtg 107220 ttatagtaat cttactgaca taacatgagg aacacaaaat gaaatcttct ttacgctttt 107280 taggtcaaga acttgtagtt gaaggcgtta ttcctgctga taacgctttt aatgaagcag 107340 tttatgatga atttatcaaa atttttggaa cagataaaaa gttcggaatt tttccttctg 107400 aaaatttttc aaagccagaa cagactgaaa gcattttcca aggtgtagta acaggtaaat 107460 ttgagtcaga agctccggta aaaattgaag tttatattga agacagttta gttgcttcag 107520 ttgctgcttt catttcattc cgtaaataaa aatatgggga cctttcggtc cccattgtta 107580 tattgctcct aatattttac tttgcgaatt gacaattcct gtcatagtat taatgtttga 107640 aatgcttcca gcagttcccc ctaatctact aagtcgtgaa agcgaattag ataatccagt 107700 aacacctcca ctatttccga gaacgctttg aatgccattt atagcagcag attcaagcca 107760 ctcaagcgca gcttgtctat caaccgaccc ggcctgcatg actctatacg caaaagtaac 107820 atcaaatgta gttatttggt tatctccatc atatgataac tcaggagcgc tcactgacac 107880 tggaatgcat ccggtgaaca tcaccgcagt atgaggtaat ccattacgag catgaagatt 107940 aacctgaata tctgcctcga catcttgcgg caaagcacgc agtccagtta ctgggtcttg 108000 aacagcgtta acccagtctt gcattgcacg atagttacaa gcttctgaat ccattctaaa 108060 tgaaataacc aaagggtcta attctctccc agttatacga atattaggag agttataatt 108120 ccagtcagtt tcataggata atctattctc tggcattttt acagaatata tcatcaatcc 108180 agatgagtta tatgccatat taaagaagtc aattaaatat gtaccaactg taaatgagcc 108240 caataaactt tgaactgtac gttgactcat ggcaccaata agatatttac taacccctga 108300 ttttcttatc agtttttgtg tgccagcagt aattagcgtg gtaattccct gattaatatc 108360 gccttgagac aatcctaacc agtctgaatt tagacccaag ttattataag aaaagttgct 108420 aattgaactt atcaacgaag agcttttagt tgatggagtt gtcgcaaaaa cgcagctaaa 108480 catattatta cgctggaaat ctgcgtttat tgcttgatta ttaaattcct ctaaagaata 108540 cattaaaaag tccccgcata taaagaagca cggtttaacg tgataatttc tctcatagta 108600 atctcaagag taaatgtact agggaggttt ggagcaatag ctaatccgtt aaagttacca 108660 ttaggtgttt tatcaaatct gatgctctgt atttgacatg ggccaaatat ttccgttttt 108720 ccatcaaact tagatgttgc accaaagttt ttcaccatcc aaattgttgg gtttgaaact 108780 actagaacgt tagttaaact cgatgtcatt ttctcaaata gtgttttatt tttagctgcg 108840 tcttccggag ataaaggttc aattaacgta gaacgatacc aatcatctaa ataccccttt 108900 atttcagcag catattgaga tttgcccgtt tcaccataag aaaaatagtt aaaatattga 108960 tagatattga taatagccat caaatcttct gttgaacgtg gagtcaaatc ccatgtaaac 109020 actttagttc tattctcagc accgccatac atacttctgg ctgtcgtata aatctgttca 109080 ttattatcag ccattatacc ttgtgttata ctttccagtg ccccaaatac tgcagttgaa 109140 gcaatattac ttaacacacc agtagcagta cctccgcctc tgctgataag actttcttga 109200 acatcattaa atctatgtga tgatgtatca acgtcagatt tagatctcgg taaaagaatg 109260 tttgcgacag gagctttact tattgttcct gaattattgt ttgatattaa tccatttgat 109320 agttttgata ctgtactact aatagtgttt ctagctgtac ttaaaatact cgaagatgaa 109380 gaagagtagt tagatctcat cgatctaaga cttccagaat ccctagatga catattgtat 109440 gcagtaaata ataatccatt cttatataga tctgttacct ggaagtcccc tgtagtgtca 109500 ttaccactag cacgcccagt tggaaactgg gcagtgtatg ttttagttgc tacttctgat 109560 ttagtactct gtccggctga aattttctca ccggactttt taattaaatc agcagttatt 109620 tctttaacaa ttgccatatt attccttaat taactccagt cgcatcaaat acaccaggag 109680 cagtcgtgct cgttacaggt gtcatattat gaacgacagt atttttctta ataacattat 109740 tagtattatt gatcgaagga gatgcttgtt gaacaggagc ttgctgtgct ttgttctttt 109800 ctattacctg gacttgtttt gcttctggcg atttagcaga agtttcaggt ttggcattag 109860 gctgattttt cttgagctct tgataagtag catcaatttt agaaaatcta gcagcaagtt 109920 cttttttaac tgccggtgaa ttatttaaat ccgggtcatc cattcgtttt ttaaggtctt 109980 cataggcagc ttcaactgat ttaaccgttg agtctttact catatcagcc gaatcagcgt 110040 atttttcaaa acgaatcatc gcggcacgag cttcattagc tttcattaaa gcattttttc 110100 tttcttcagg tgaaagttgc tttaattttt cttcttctgc tgcgcgttct tcatcggttg 110160 tcaatgcttc tttattatca acgccacgaa tccagttaga tgcacgggtt ttccaattag 110220 caattttatc taatcctttt gctattggac caagatcgtc attcattcgt ttatcttgat 110280 aatttgctac tttttcttgg tcttctttat tgagagatgc tccagtagaa ttttggaaat 110340 tttccagcgc tcttccttct acttcatcag cggtatcctt cataccagga ataactcgaa 110400 gaattgctgc tgataattta gccattccaa gctgaataag ttctcctaaa ttataaagaa 110460 cctttccaag cccttctaca atagctaccg tcaaaccgcc ccaatctccc tcttcccaga 110520 attttttaat ttcatcaata gaactaaaga tgctctgtaa taaaggaccc catgttccgg 110580 tttcgctaga gaatttagta aagtttgaac taaacaaatc ccaagcctgc gaaaatttat 110640 ctgaccaata tttaaagtga accatcagca aatctattcc aacaacaaca gccaatatca 110700 ttgcagtcat tttagcagct tcaatagcag cactgacagt atacttaaat agcatgcttg 110760 atattttatc agtaattgaa atagatttct taaatccgaa atcaacagtc tttgttaatt 110820 tatctaaagc ttgagataat tttaagttaa atgcgtcttt tttctgtttt tcttctggcg 110880 attcttgttt gggttcaact ggctgagggg tagggaaaaa atcagcatca ggatcattat 110940 taactgcttc aggagccggt aataaaggac ccacggattc agctgtatcg tcctcaacaa 111000 ctttaacagg aatagcgctt tcaaccgtag ctaaactagt tcctgtttgt tgaattccgg 111060 ctgtctggat tttttgctct agtaaactcg ttaatttatc taatttactt ccgagcgatt 111120 cgccaatttc tttattaata ttgttgccaa tttcgacagt ttcagcaatt aactcagaac 111180 cagcagtagt atcactcact gcgttttcta cgttgtcaat tgctccaatt atttcattcg 111240 atttttcttc gacagtttga gcaattaatt cagaagcagc ttgagcatca tccaatttcg 111300 tagatatatc gttaagtcca gatagagtat tggaagcgga tttagccgct tcctgtgttg 111360 gtttattatc tgaaataact tttctacgca tcgtttgcat ttcttgtggc tttttcattc 111420 aaataatcca ataatattgc caattccggt tattggacca ttagggccag gaattgctaa 111480 agttgtaaaa atatcatttg cccattttaa aacgaatgct ggcatctcaa gaaaattaat 111540 ttctttaact tcatcattga ccttaagcaa gcatttggat aacatatcgc tcaccgttaa 111600 aaattgttca aattttccag gaggtctaaa ataaaatgta tttccttggt attgaaattc 111660 caatctttgg catacataaa catcattaat gtcataagta taaccatcta tttctttacg 111720 agatttaatc tttccattaa attccaatag atgaatagaa acgaaatcaa cttctgccgg 111780 tgataaattc ggacaaatag aatcaataag aagttttaaa ttttcatcag gacctttaac 111840 atcttttaaa atgttataat gtttaagacc cattttagga atagaaactt ctttattgct 111900 tattggaaga actactttct tcagtggtag tatcagattt aaattcattt ttaaccttaa 111960 ctgggtctat tgtttccagt ttcgttgcat tagtgaacat atatagatga gttactgaat 112020 tattattgga taattcatgg ataacttcat caacgtaaaa ttctgtttta aattggtttt 112080 tactatcatt aaaaataatt ttaacgccag gagtcaagtt aaaattaccg acagtagaac 112140 atttagcata accatcgtat tgtgccatag tttgaagacg aatagcttct tcatatccat 112200 tcctataagt catttcagaa taagcacctg accttgatac tacaatagag ttttctccct 112260 ttcctgtagt aatcattggc aatgaagaat ccaaaaacga atgcgcatag atagtagcgt 112320 ttttcattgg gtcacgtttg tgaggattcg atttagtcaa ccaaacgaaa tcatatgcca 112380 atggatattt taattcttgg atgaattgac ctattaaaga tggctcaccg acaatcattg 112440 gatatggttc ttgatttatc atcatatcat agtccatcat gttaactccc atgatgtctt 112500 gccacacaaa tacaaatttg tcacttccta cagctagagc aacttctctg acatatgaca 112560 aatagttttc aaatgtacta gtccacggaa tatcaggaac ataagcattt attgcattta 112620 ttgctggagt taataacgtg cgatcctgat aaatgacacc aagcatttcc tttatagatt 112680 cacctgcatc agagaaaaac ggtctgccaa atttaagatt ttctatagaa tgaatagttc 112740 ccaattcaat agcaatgatg ttatcaccct ttgaatctac agatacagaa aaatgcttac 112800 atccataaat tcgtgtttta acattattaa tatcgtttgc attagctaca gaaatctgaa 112860 ttatttcatt tccatccatt tttgtatgga tatttttaga atcataaaac tgcagcattc 112920 cttcatttcg gccataaaga gaatcccgca tagttaatgt ggtaatagta gcagctaatt 112980 caacaaatct attattactc caagcgtcgt agctatcaaa taatttaacg ctgagatttg 113040 gatatcctgg gcgttgcaac atactcatta ttgtttatcc ttctcaatca gttttaatac 113100 gaatccgcgc tcggcaggaa tcattttcat tattgaattt aagctataat tactttttac 113160 gagtgtgtga ttaatttgat aaaaagtaaa tatctcatct gggttaacta atagcttaaa 113220 cacatctact atatcagtgt atttttttac atacttatta caacatgaca tatgcaatgt 113280 taaattaata ggattcattg catcgagaat tttttctaat gtttctatct cgatggcatc 113340 aacaagttct atttgacttg attcgctaat ttcattccaa tcataccaca tttcatctac 113400 ttgaacggaa tgaatatttt cagtaatcat ctttgcttta ttttcataaa actcagaagg 113460 aaactttaat ttaattttaa cattagctac atcaaaaaca ggttccttta attctttttg 113520 atatatttca aatggaactg tcttttcttt tttacatttt ggacatacaa atgtgaccgg 113580 tactttagtt ttacctattg accctacaaa tacctgcaaa aatataaatg gttgccaagt 113640 cttcggatag tctccaaaat aatcggcaat taaatcagca attatttctt tttgttcttg 113700 tggtgaccga tgttctatat cgtttcgaac taacaaaaaa tctcgataat cttctaccgt 113760 aaatggttta aaacgatgaa caccatctgg taatttacaa cgaataatgt ttgccataga 113820 tgctcctttt attctattta taaatatgat aaataaagga gctaaatatg tatgaataca 113880 aatttgatgt gagagttggt tctaaaataa tcaactgtcg cgcatttact cttaaagaat 113940 atctagaact tattactgct aaaaagaacg gttcagtaga agagatcgtt aaaaagctaa 114000 ttaaagactg cacaaatgca aaagatttaa accgccaaga atcagaacta ctgttgattc 114060 atttatgggc gcattctctt ggagaagtta atcacgaaaa ctcctggaag tgcacctgtg 114120 gaactgaaat accaacccat ataaatctat tacatacaca aatagatgca ccagaagacc 114180 tctggtatac actgggtgac attaaaatta agttccatta ccctaaaatt tttgatgata 114240 aaaatatagc ccacatgata gtatcatgta tagaaacgat tcatgctaac ggtgaaagca 114300 ttccaattga agacttaaat gaaaaagagt tggaagattt atattctatc atcacagagt 114360 cagatattgt agctataaaa gatatgcttt taaagcctac tgtttatttg gctgttccaa 114420 ttaagtgtcc agagtgtgga aaaactcacg ctcatgtaat aagaggactc aaagaattct 114480 ttgagctatt gtaatggcaa atattaataa gctttattct gacattgacc cagaaatgaa 114540 aatggattgg gacaaagacg tttccagatc tcttggatta aggtcaatta aaaatagcct 114600 tttaggaatt attacaacca ggaaagggtc aaggccattt gacccagaat ttggatgcga 114660 tttatctgat cagctttttg agaatatgac tcctcttact gctgatactg ttgagcgtaa 114720 cattgaaagc gcagtaagaa actacgagcc acgtattgat aaattagcag ttaatgtaat 114780 accggtttat gatgattata ctctgatagt agaaatacgc ttttcggtca tcgataatcc 114840 tgatgatatt gagcagataa aactacaact ggcttccagt aatagagtat aatgcttcac 114900 gtataaacgt ggtataatga atctaagtcc atccaataac aattgaatag agaacaatat 114960 gaaattagaa gatcttcaag aagaattgaa gaaagatgtg tttatagatt cgactaaatt 115020 acagtatgaa gcagctaata atgtgatgtt atatagtaaa tggctcaata agcattcaag 115080 tattaaaaag gaaatgctta gaattgacgc acagaaaaaa gttgctctta aagctaaatt 115140 agactactac tcgggacgag gagatggtga tgaatttagt atggaccgtt atgagaaatc 115200 agaaatgaag acagttctat cagctgataa ggatgtttta aaagttgata cttcattaca 115260 gtattgggga attttattgg atttctgtag cggagctctt gatgctatta aatcacgcgg 115320 atttgctatt aagcatattc aagacatgcg agcatttgag gctggaaaat aatgaaaagc 115380 attgatgatg cttttaatta tgaagaagaa tttgaaacgg aaattcaatt cttaatgaaa 115440 aagtataatc tcaagcgtca ggatattcgt atcctggctg accatccatg cggtgaagat 115500 gtactttata ttaaaggaaa atttgccgga tatcttgatg aatattttta ttctaaagat 115560 atgggcattg atatgcatat gagagttata taaatagata tataatttag aggagacaat 115620 catgtcagat aagatttgtg ttgtctgtaa aactccaatc gattctgcat tggttgttga 115680 aacagacaaa ggtcctgtac atcctgggcc ttgttataat tacattaaag aactaccagt 115740 ttcagaaagt tcggaagaac aattaaatga aacacaactt ttgctatagt gtgaccttta 115800 gtctatagtt ttggcccttc ctttttggtt gggccttttt taatttaaaa gctttcttct 115860 acttcatcgt ctgaatcttc taattcagct ctttttcctg ccaaagcatc tctcacagag 115920 atgtcatcag tatcttttaa ttcagtttct ttaactcttt tcttataata agcttcaagt 115980 tcttctagac cttctaatgt ttggcaagag gcaattttac ccataaattc atcaatagaa 116040 gcttcataaa gaaattgttt aaattctagt aacatctttt tctccaaagg gccgaagccc 116100 ttataaatta actgtttttc taagtattct tagtttgtac attgataatc cagtagcttt 116160 agaagcatct ttcatacatt tatacttaac accatttact tctataggtt tagcagctgg 116220 gttattagct cctgaattat ctggcatatt atctttaatt ttctgaatag atttagcgga 116280 atgtttcctt ccatacatgc cattattttt acctgattga ttgatagaat gcaatctttt 116340 agcttcttca gtccattttg ttgggttcat tcttttagtt ttactaattt tacttcccca 116400 tgtaattgga cgatttttca tgagttttga catatattgt ttatgttctt ccgtatggtg 116460 ttttccgtaa aatggattac cttcacctga ttgaagttct gccattttac gacgttccca 116520 ggcgtaagat ttattaacgt gtctttcatg agtctttgaa ttcatcccca tacatataac 116580 ggctttaatt ataccgtaat ggtttggatg aattttagct aaaagtttat gagctataaa 116640 atgctcttca gctgttagtt ctaccaaatt acacttatca tctgtaccgc ccatacatct 116700 aggaattata tgatgagttt cagtgtatcc attaaggcga gcccgattct gggctctatt 116760 aattaaatta tcgtatatta atttataatt caaagtttaa cctctttcat cacataatta 116820 aacttctcat cagcataacg ctgaatacga tcaagtccat gttttaaaag atagtttaaa 116880 tgcgagtatt ttttagaaga tgtttttgat ttagaaacta cgcccgcgtc atctatgagg 116940 tcccagaccg ttgcgattgt tttagaacca tgcttacgta atacgcgacc tattgtttgc 117000 aatacaataa ttttagattt aacaccgtgt gctaaaacaa cgtgatgcag atttttaact 117060 gaaatgccag tagaaaatac accataacta gctactataa ttattccttt accgttttca 117120 gctaaggttt tcattatatt gcgggtttcg gtatcaactt cccctgatac gtaataaact 117180 ttatcgtatt catttttaat taaatcgaaa atagctttac catgtgatac atgcttaaac 117240 atgacaaaag cgttttcatc tttttgcgca agcttaatag ctaatttagc gatccattta 117300 tttcttttac tgagtccagt aataattttt atttcttctt ggtaagtttt tccctttaat 117360 ttagtagtga actcatcggg atagcgaaga aaaatactat taatttttaa ctcagttact 117420 tgtccatctt ccattaattt agaagtcgtt actggcttaa atatttcacc gaacattcca 117480 acatactgca tgatattagc tttgccatca cgtaatgaac cagacaaacc gaatttgaac 117540 atgcagttat ttaaacctga tatgatagat gaaatacttt ttcctgtggc aagatggcat 117600 tcatcattca tcatcattcc aaactgtgag aaccattctt tcggttgttt tactacagtt 117660 tgccatgtac caacaacgac tggtgcatca tttttatatt tatcatcttt tgatgctcca 117720 ccgccgattt tctttatcat tgcatgactg aataaacgat agtcaacaaa gtcatcagcc 117780 atctgagttg tcagagcagt tgttggaaca atgataagaa ttttaccttc ataattttcc 117840 aaataataac gtgcaagcaa agcttgaatt aaggatttac ctgcagatgt tggaagatta 117900 agaattctac gacgattaac taatccttcg aacactgcat ctttttgata ccaatgcggt 117960 tcaattcttt tatttcctga atagatttct aatttagaaa gccattcatc aaaatctttt 118020 cttgataatt cttctttttc gttaatctgt gggtcaatcc aggctttata gccaaagtta 118080 tcacagaact ttttaatttg cccgactaag ccgaatggaa gaagacgatt ataatctaaa 118140 agacggattc gtccatccca gttgccatat ctgaagcgag gattaaacct atatccatcg 118200 gcctcaaacg aaaagaaatc tcttaattcg tggaacgtgc tctcttcaca atcgatgcgt 118260 acatgactga agtcgtgaaa atgtacttta atatccataa ttatgcctta ctaaatttgc 118320 ctttagaatc tcttttcatg agacgacctt taataaatcc gtcgggaata ataccgtctg 118380 gttgtattaa tttatttatt gcgccattat tgacccaaaa agttcctgtt gtagtcggtt 118440 tgactttaca tccttttctg gactttctat taggatgaac catccctttt acaaatcctt 118500 ctggaacaag ttctccaggc ttaataaaaa tatttttagt tccattagtg taacaagttg 118560 tacctaatac cgtgcccggt gagttttcaa atctcttagc ggaagattct ttcatctttg 118620 ctataacctc tgtagtcata acaattccac caattccacc aggtttcata ttataataat 118680 ttttgctttt tatcagttca ggagttataa tttcttcttc atacatgtac gcttcttcgg 118740 aagttttaaa ctcttttagt atttttctag agaaattgtt ttcaccatat ttctttatag 118800 cctgctgaat tgccttaccg gaaccaaggt agccatcatt caggtcgtca gtagagtgct 118860 ttcctatata ctttttacca tttattagat ttgttgtttc gtatacaaag tggtacatac 118920 tattttccga gtaataaata tatctatatt tatactgagg aaatattatg atagataaag 118980 attatattgc agagctgaag gctcttgatg ataataaaga agctaaagct aaattagctg 119040 aatatgctga acagtttggt ataaaggtca aaaagaataa atcttttgat aatatcgtta 119100 atgatattga agaagccctc cagaagctcg ctagtgaacc tatgccagag actgatgggt 119160 tatctattaa agacttaatt gatgctgctg atgctgcaga cggattaaaa tatgacgatg 119220 aagaagtcaa tccagaggca gcacttctga ttgattctcc ggttaaatct gacattaaaa 119280 ttgaagtagt agaaacggat aaaattcctg aaaataccga tgttttgatt gaagatactc 119340 cttttgttga agaaaaattc gagcaggctg tagctgagat tattgaatct gaaaagccgt 119400 ctgtatttac tcttccggaa aactttagtc cgaatcttca gctgattgga aaaaatccag 119460 gattctgcac cgttccttgg tggatttacc agtggattgc tgaaactccg gattggaaat 119520 ctcacccaac tagttttgaa cacgcgtcag cacaccaaac tttatttagc ttaatttact 119580 acattaatcg tgacggatca gttctaattc gtgaaacacg taattcttct ttcgtaacat 119640 taaaataagg ataacttatg gcttttacag ttgatataac tcctaaaaca cctactggag 119700 ttatagatca gactcagcag tttactgcta cacccagtgg tcaaactgga gatggaccta 119760 ttacatatgc ttggactgta gatgatgttc cacaagatgg agctgaagca acttttagtt 119820 atgtactaaa aggacctgcc ggtcaaaaga ctattaaagt agttgcaaca aacacagttc 119880 cagatactga tgctgaaacg gcagaagcta ctacaactat cacagttcaa aataagacac 119940 agacaactac cttagctgta actcctaata gtcctccaga aggagtaatc ggaacagctg 120000 ttgaatttac tgctgcctta gcttcacagc catcgggcgc atctgctacg tatcaatggt 120060 acgtagacgg ttctcctgtg ggcgaagcaa cttctgctac attcaattac actccttcca 120120 caagcggagt taaaacaatt aagtgtgtag ctcaagtaac cgcgacagat tatgatgcaa 120180 aggaagttac ttctaatgaa gtgtcactga ctgttaataa aaagacgcag acaactacct 120240 tgacagtaac tcctgatagt cctccagcag gagttattgg aaccccagtt caatttactg 120300 ctaccttggc ttctcaacct gatggagcgt ctgccacgta tcagtggtat gtagatgatt 120360 cacaagttgg tggagaaact aactctacat ttaactatac tccaactaca agtggagtaa 120420 aaagaattaa atgcgtagct caagtaacag cgacagatta cgatacaaaa gaagttactt 120480 ctaatgaagt gtcattgaca gttaataaga aaacaatgaa tccacaggtt acattgactc 120540 ctccttctat taatgttcag caagatgctt cggctacatt tactgctaac gttacggatg 120600 ctccggaaga agcgcaaatt acttattcat ggaagaaaga ttcttctcct gtagaagggt 120660 caactaatgt atacactgtt gatacatcat ctattggaag tcaaactatt gaagttactg 120720 caactattac tgcaactgat tacgatagca aaactattac agcagaaggt caagttcagg 120780 taactgataa agttgctcca gaaccagaag gtgaactacc ttatgttcat cctcttccac 120840 atcgtacttc agcttacatc tggtgcggat ggtgggttat ggatgaaatc caaaaaatga 120900 ctgaagaagg taaagattgg aaaactgaag atccagatag taaatactac ctgcatcgtt 120960 acactcttca gaagatgatg aaagactatc cagaagttga tgttcaagaa tcgcgtaatg 121020 gatacatcat tcataaaact gctttagaaa ctggtattat ctatacctat ccataatcat 121080 aggggcttcg gcccctttct tcattttgaa agcacacaaa acacaatcgg aaaacgatgt 121140 atataatggc accaactcga taacatgaga ttgattatga gaactgaggt tgtggtgttt 121200 acactccatg agtctggaaa gtcattcatt gaaattgctc gtgaattgaa cttacaggca 121260 aaggaagtgg ctgtattatg ggctcgagct atgactgcta agaataaatt tgagactcga 121320 gaaaaagttg tctatagaaa aagacatatt aataaaaagg tgaaaaatgg aacagtatga 121380 tctttatgaa aatgaatctt ttgctaatcg attacgcgaa aaagcactta aaagtaaaca 121440 gtttaagcta gagtgtttta ttaaagattt ttcggaactt gctaataaag cagctgaaca 121500 aggtaaaaca tattttagtt attattgtac tgctcgcgat aaattgatta ctgaagaaat 121560 tggtgattgg ctgagaaaag aaggatttag ctttaaagtc aatagtgatc agcgtgatgg 121620 tgattggtta gaaattacat tttgaggatt aattatgttt aaaaagtata gcagtcttga 121680 aaatcattac aactctaaat ttattgaaaa actttacagt ttaggattga ctggcggcga 121740 atgggtagct cgtgaaaaga ttcatggcac aaatttctca ctgattattg aacgcgataa 121800 agcaacttgt gctaaacgta ctggaccgat tcttcctgct gaagatttct ttgggtatga 121860 aattattcta aagaattatg ctgattccat taaagcagta caagatatta tggaaacttc 121920 agcggttgta tcttatcaag tctttggcga attcgctgga cctggcattc agaagaatgt 121980 cgattatggc gataaagatt tttatgtatt tgacattatt gttactaccg aaagtggtga 122040 tgtgacttat gtcgatgatt atatgatgga atcattctgt aatacattta aatttaaaat 122100 ggctccactt ttaggtcgcg gtaaatttga agagcttatt aaattgccaa acgatttaga 122160 ttctgtcgtt caagattata attttacagt agaccatgct ggattagttg atgcaaataa 122220 atgcgtttgg aatgccgaag caaaaggcga agtatttacc gctgaaggtt atgtattgaa 122280 accttgctat ccttcttgga tgcctaatgg caatcgcgta gcgattaaat gcaagaactc 122340 taaatttagt gaaaagaaaa agtctgataa gcctattaaa gctaaagttg aactgtcaga 122400 agctgataac aaattggtgg gaattttagc ttgttacgtt acactgaacc gagtaaataa 122460 cgttatttct aaaattggcg aaattggtcc gaaggatttt ggaaaggtga tgggactaac 122520 tgttcaagat attttggaag aaacttctcg tgaaggtatt actctaactc aagcggataa 122580 tccttctttg attaaaaagg aattagttaa aatggtacaa gatgtacttc gtccagcttg 122640 gattgaattg gtaagttaaa taaaaaggga cctttcggtc cctttgtttt attcatcaac 122700 gataattttt ggtagcttaa cgcctaataa aacagacaaa tctgaacgac ccgccatttt 122760 atccatgtct ccaccatcaa ttattcttgc ttctttttcg tcttttgcta cagtgtaagg 122820 atttgcagat aaagcatatc taaccaataa accgatagat ggctgcaaac tttctggatc 122880 aactacaact ttaaatgcac ctacatgttc agggtcatct aagtcaagac cttctgtata 122940 cggagcatag aaaattgatc cgacaatttc tttttcgcca atattttcta ccacgccaac 123000 gattacataa tctaatgggc tgttagtatc gcaataaagc ggtaaaccat tagctaagaa 123060 cccataggca ttttgtgaaa gatatttatc atcttctggt ttatgtttta accaacctga 123120 tgcagcaaga atcgcagcgg cacgagctga agcaacacag aacgttgctg tataagttga 123180 ttctttttgg atatgcgaaa ccatttcaca caccattcgg tataatgaac gaccagcttc 123240 aggtgcagat gcataactca aatcaatgaa tccagtatca gtaattcctg taactttata 123300 gcgttttgac actgtaatca aagactgtag aatatcttta ttgatttcat ccgccatttc 123360 agttgcaagc aaatcttcca agaaattagg agcatcgaat ccatttgctt ctaaatcttg 123420 tgctaattca actgtaatgc cagttttaag tttacgagat ttaactgcgg tttgccattt 123480 attaatctgg aatctagcat cagcaatttc gctatcagag ctttcaaatt tgctcgttga 123540 cgctgcgtca gaaaatagac gaacctttaa aagaacaatt gcaatctgaa gagctagttc 123600 taaatcgctt tcttcaatgg tagcaaatgg tgtatcttct aatactttat aaacgatatt 123660 attatatttg aataaatcgc ctttattgag agttaattta gactcttctg ttaattctgt 123720 gatttgttct cggtctacat acccagcttc gccagcgtaa gtagcaccag ttttaaatgt 123780 aaattcatta tctgggttaa ggtatttgat accataaaaa gcagcaacag gttgattagt 123840 tctttgcgtt gctacaatgt cagaatatat taatttagtg gtagcgcgag tcaaagcaac 123900 gagatttggg cgaccgattg agttgctatt cgttgtggtt gattcgcgca gaagttcgtt 123960 gattttagcc attgcgcttt ccttttggtt tatatgattt atttatacca taaaaacaac 124020 taaagggacc cgaaggtccc ttaaatcgtc aaagattaga tacctttaac atatacacgg 124080 cggaagtaag cgtttttacc aaggctattc agaatagaag gcataccgct ctggatgcga 124140 gaagccggag cctgagcagc ggattctgca aatgggttga taccgatacc gtaacgagtt 124200 ttgaatccca ttactggttg gaagttcttc ggatcggaac cacgcagcgg agtcagagct 124260 acatatggag catagtaaat accagcatcc atttcgttcg gacctttata acctacagtg 124320 aaataatcct gtttagcata ctggtcgata tatacacggt atttaccacc cagaacacca 124380 gcaaatactg acttggtagt atcagtgtta aagccggtag ccagaccttg tgcagcataa 124440 gaaatgccag tatcaactga agccagaacg ttaactacgt tacgggaagc gataatgaag 124500 ttaccttcac cacgaccggt ctgacgagca atttcaactg cttctttgtc aatctggaac 124560 aacagagctt taaaggattc acccgcccag cgagcaccac gaatatcaat cggatcctgg 124620 aagtcaaata caccagcttt agaacccgga gtcagggtca taccagattt accaacctga 124680 gctgagtagt taatccaatc aacaacttca cggttgattt ccagcatgat ttcggtagcc 124740 agaataccag acagttcagc atcagcatcc ataccgtgaa cagcgcggag gtcttgtgct 124800 aattcgatag agtaagcagc tttcagctga cgagatttag cttcgataac ttgtttatcg 124860 atacggaagc ccatttcatt ccatgggtta tcggtagaac cgttgaaact ttcctgaagt 124920 tcagcgatag aagtagccat accttcagcg atttctacca gtgcaccagc ttccatttgt 124980 ttcttaattt cagcatctaa tttagctgca tcagttgcgc cagcatcgat tgttactaca 125040 gcagaagcct gcagatacac agtaccagtg tcctggaaga agtgagtata gatatcaccc 125100 acaacggttt gtgtactagc agccagagct gggaatttct tagcagcacc ctgaccagag 125160 aacattgcat ctggaccata cattgggtgg aatgcttctt tagcaccggc agcgattggg 125220 tctttaccat atactgcgcg cagtgcaaat acctgaccag tcgggctgtt catcggctga 125280 acaccacaaa tatcgaaagc aatcaggtta ggaatagcac gacgtaccat acccataaca 125340 gctgggccaa tctgagttac tgcgccagaa gtctgaccag cagcgatgtt agtagcattg 125400 taaccgtggt caccgccgat ttcagcttct gttaagaaag aaccaaatgc ctgagcaatt 125460 ttttcgtctt tgtactctgg agctgtctgg aaatcttttt cctggttttc aaagatttta 125520 gcgataatcg cttgtttgct attagcaatt tccggtaaac cttcaccttc cagtaatggc 125580 ttccatttgt tcaaaagttc agctttagtt ttgatagtca tttgtgttaa cctttaaaat 125640 tagaaacgag atgcgacttt cgcatataca cttacaatat cttctgcacc aggtgcagct 125700 ttatcttcta cagcttcagt gacgaaattc agtccggctg cttcagtatc aggagtattt 125760 ataccctcag taatagtgct ttcatcttta ttagacttct tcaccatttc tacgatggca 125820 ctcaatttac ttgagaatgc atctgaataa tccatacctt cgaccagagc agagactttt 125880 tctttttgag actcagtcag atctttagta ctttcgctca atgccacttc acgctgcaca 125940 taattgatat atgcgtcgcg catattgagt tcttcgaaca gacgagctga ttcttcttta 126000 tgttcttgca gctcttcttc catttcagct acaacatcaa ctgattcttc tggaacaaca 126060 acgttgtgtt caacaaagag ctcttttaat ccaccaagca tggattcaaa cagttcggct 126120 ttgatgcctt tatcaactgc taatttattt tcagcgagcc attctttcgc aagatggtcg 126180 atgaatttag aagcttgctc agcgattttc ttctcagctt tttcttcggc ttcttcttta 126240 tttttttcta cttcttcttc tgctttttca gcaattttag cgatatgaga ttcagctaat 126300 ttaacggcgt gctgcttgac ggtagcttcg aatacagtgc cgaaagtttc ttttgcttcc 126360 ggagaaatat taactgattc gaaaatacta tcaagagcaa cggaagcatc aattttttgc 126420 gcttcagcaa tcagttgttc tttaagcatt ttgtagtcct gttgtttaga taataatatt 126480 tataacgctt ttttcatggc ctctgcgaga gccatatagg cgtcatcggc acttgtatcg 126540 gcttccgccg tctgtgattc ggtaatttcc ttaggagtta cccatgcatc tggagcactt 126600 ggaccccata ctgcatcaac acctacagtt aatttgaatc cttcgtttac gatacgataa 126660 cctttatttg tgtcagtcaa tgaacctaat ccacgagaag aaactcctgg aatccatccg 126720 gcacgaatat tagctgctaa tttatctcca ggaccatggt caccttcaat aacacgagct 126780 cgtccgtata cgtcatttcc tttccaccac atatcttcta taatgatagc ggcttgcatc 126840 gggtcaacat tagcgcgtgg aggatgattt aattctccga gagcttgttt agttaaaact 126900 tgctcattga tatagtcttt taccgctttt tctaatatgc gttttggata aagacgttta 126960 tttctattga cgacttccgc ttgcatgaat attccttcga tgtataaacc gggttttaaa 127020 cctaggtctt ttccatcatg agattcaagc attggcacgc catcaataat ttcgccaggt 127080 tgaccccaag tttcaattag taattggggt tcattcatta gcttaatcct aatgctttac 127140 ggcgtttaag agctttttta cgcttacgct gagcacgaga ttgacctgct ggattggcaa 127200 tcttcgtttt ggtagctttg cgagcaattt gtctacgttt tgctttagac agcccggtgg 127260 tttgaaatgc attacgttca cgcgtcttgc ggtctttagt acgtgtaatt tcaccacgag 127320 cagaaacatg tttaacgatg aattcattta gcggcatatc ttcattaata gaagctaatg 127380 caattgctaa atcagtttca tcatcaagca tattctcgac aattgtattt atatcgtctt 127440 tatttaaagc agaagacaat tcgtcaaagc gaccctgtgc ttcaggaata agtgcttcga 127500 cattctcgag aactaattca tgagtttcag ggatcagaag cattattcat catcctcgtc 127560 ttcgtcagag tctttgtctt ttttgtcgtc tttatcatcg ctatcttcgt catcatcctc 127620 gtcttcgtca tcatcaggtt cttcaccttc gattaagaaa ttacgagcga tagcgatttt 127680 ttcttcttta attaaatcaa tcgttcttgc agccatggct tcagcaaata atttacgagc 127740 ggctacgagg tcgtttgatt taatagcttc aattaaacct tccattaaaa atcctcttgt 127800 tcttggtcgg ggtcttggaa acgagcctct ttagactctt cttcaatttg cttggcttct 127860 tgttctattt cttcatcagt catctgcaaa atatctttca tagcagttct gtgagaaata 127920 tatttaccaa taaatggttc tgccatggtt agcatattaa ttcttcgttc caaaatttct 127980 gcttctttga gttcagtaaa gtagctatcc cgatgaaatt ctatcttaat attatttatt 128040 tcatcattcc actcatcttc tgtgattata cctttaagca acagatttgt tttaagcgga 128100 tctaggaaaa cttcttcaaa cttgtgctgt aactcacgaa taaatttagc aaacgttaat 128160 tcatcacgtg taatgctagt tccagaatca aacatcacac cgccttgttg gtcttgcgga 128220 atgcgtgaaa gaggaacacg taatgccata taaagagctt gtctaaacca acgaacatct 128280 tccatattac cggtgttatc agcaccagga agagtatcaa cttctgtcac agctttacca 128340 tcacggcgct gcaaccaata gtcttcggtc atagacatat tatgctgttg attttttatt 128400 ttacctgtcg atgcgtcata tactacacgg tttttcatcg tgttcataac atgttgcatg 128460 tgctcggcag ctttacgagc aggcatatta cctgtgtcta cataccaaac acgacggtca 128520 ggagcgcgag taatacgata aatgactacg gcatcttcta ataattttaa ttggttagca 128580 ggtttaacag cacgatgcaa atacccgatg atatttttac cacaacaatc gactaatcca 128640 gaatgggcat aaacgacggc agctttagga atttttattt ttgtgccagc ttcatacatt 128700 ctaccatcac atgcatatga ttcatgtgca gtatcatata taaaatactc tttgtaacct 128760 ttaactattt ttgtgccagc ttcagtttct gttataattt cacgaacata ctgaacttga 128820 cgagggtcta atctacgtaa ttcctttatg ccttcttttg gacgttttgg atcaataatt 128880 ttatgaaaga aaattcttga atcgacatac caacgtctaa aatgatcaga accttttcgt 128940 tgaaacgata gatgatttaa tacatcgcta aattcatcta acatcatatt tttaattttt 129000 gggctaaatt tagatttatc caaatttaac gctacgactt cagtatcatc ttcatagacg 129060 atagcatctg aaacgatttc tgaaactgca ttatctactt catagttatt catgagatta 129120 cgatatgtat caataagctc acgagtggtt ttcattcctg gttcatatga accaaaaatt 129180 gtttggaatg cagcattata aggagaagca gcttcattcg agcttacttc aaattctctt 129240 gctccatcat caagctttgg ggctgtaatg gaaacaagat cttctttttc ttggtcttta 129300 aaatttcgtt cgtccatttt agcccatgga gcaaacaaac ttaatacatt aaatttcatt 129360 gtattctcca aatgggaatt atagttatat ttataatgga cttctctgct taaagcagga 129420 tggggatttc tccccattca ttttattccc aataatcgag agcaagagtt acttcaaagg 129480 tttggatttc attgtttgaa tcccaatcta attgaagttc acccacgtta gtaggccaca 129540 ggcctttaat ttcgacttct tttgttacag ttttagcatc acgagcatat tggcgaacaa 129600 tagcgctctt tttatactct gctggttttc caccagtaat ttcattcccc tgcccagcag 129660 caatgctttg ccaatcaaca aacttctggc gagcatcatg tgcctcatcg ttcattactg 129720 taacagtcca gtcatcgaat gtacgatcgc ctgctacgtt aattttacgg ttcataaatc 129780 cgactggaat tttttctaca ataccagctg gtaaagcagt ggctttacat tgaaacgtaa 129840 aattttgtcc aagataagaa atttctactt ggaataagtt aggtcgagca aaatcaccag 129900 attcaaacgc tcgtgttaca tcatctacaa acatattagc ctctgtagta tttatatccc 129960 tatgtttaac ctagggcata tagaattaaa gaattaagga tatagtgtat ttatatggcc 130020 tgccgaaaca ggcctttaga atgcaccgta ttaacctgca agaccagtta actcatcgaa 130080 atctgcacca gtagcagttg ctacgaagtt taaggtaatg tagttaatgc ttctagccgg 130140 ttgaatgtag aatgttgcaa caaactcatt tctatcaatt actgacggag tgttatttgt 130200 tgtatcgcaa actacacgat attcataaat tccaccgaga gctttaattc cctgcaagta 130260 ctgggaagtt tctgtgcgga acgatgaacg agtaaacgcg ttgtttaatt cgaacaaacg 130320 atattttgaa ctacgtccga tattcgtttt taacatatta aacagacgac gaacgttaat 130380 acgatcaaat ggagaaggaa cagaagtagc tgttttatca ccatataata cgtaaccatc 130440 accacctgta ccagttactg ggttgatagc ttcttggtat aaacggtcgc gctgcgcctg 130500 gcgagtttca atagcaagtt taataacgtt aagaatctgg ccacgattat aaccagccgg 130560 agacatccaa gtctgagaaa cgttatcagt tcttgcgcat aaaccagcaa tatcagctgc 130620 taatggaacc caacgattca catcattata tttgtcatac tgatacttgt agttaccatc 130680 gattgctgca taggttgaac tgatattaaa gttgttatca gtgtatgaac ctgctgcagt 130740 tctccaattg actaagttat caacagcacg agttacagga attccaacta cagtttcgcg 130800 cggcggtgag cacaatacta agcaatcttg gcgagcatca ccaattgaaa cgacatgttt 130860 ttggacagta gatgctgttt ctaaagattc gccggcgcaa gaacccgcaa taaacaactg 130920 aacgtcaaca gattcacggt cagcaaagaa gtcccaagct tccatcaaat ctcctgctgt 130980 tacttcagca tttgacgata atccaccaga cagagttaaa attccagaga agccttctgg 131040 ccagttttgt gcagttgcaa aaatatattc tgaaccacct tttgcgaaaa agtcatcaat 131100 atagatgtta ctatcgtaaa tatctttttc accacgcttc gttgaaagaa caacgctttg 131160 aacaatagca tcattgcgac gaactataat agcgtattgt gaatcagttt gtggtccgta 131220 tccaaacact gctttggcag tagatgcgcg tgtgccgccg cctggataaa ttggaagtaa 131280 tgcagaagca ccttttgcgt agtcagcttt agatacgatt tcaatttcga ttttatcgcc 131340 caactcgcct ggataaagag ctactactcc tggaattcca tatttttcga gatttgcttg 131400 aaagtcaaca gctgtcatgg cagcttcagc attttcaatt tcagctaata aaataccaga 131460 atcagtaata atttttccaa gagttattac tgcagctaaa ccagaggaag acgaagaaat 131520 ttccgcggtc cagttagaac ctaatgttgg atattcaccg acttctttcg ctttagcgat 131580 aatttttgcg gtaggaatat taattttctt aatttttcca tcagcatcta cttcggtaac 131640 tttacattct gtttcaatgt cttctgaaac atacttaacc gtgattttat ctccaaccgc 131700 gtagttacta cctggggtag aaatagtgta ttcaatatta ccggcaattg gagatgagtt 131760 tttagcggta tctctatcga cagcacgcac aactcgcaaa tcatttccgt attgtaagaa 131820 attcattgca gacataaaat agtcagcagt ttcagctgta ggttgaccaa aagtattaac 131880 taaatctact tcatttgtaa cctgtttaat ttgaaaagca ggaccccact ggaatttacc 131940 ggccaaagct gctgtaccag tagagttatt aaccacggtg ctttgaaccg tagtttcttt 132000 gagctcaatg cccggagata ataaagtcat ttttaatcct ctttaatatg ctttaatata 132060 tttataccat tgacatacca tgagatactg gaacatactc agcagaatga accgaatcaa 132120 caaatatgac tggagcgtat tcatcaccca tatcttggag ttcttttgaa aacacttcag 132180 atgctagtcg catgtcatct ttgtcagcat aatcaataaa ttttgattgc gttgataacc 132240 atccaaaaat tactaaagac attactaaat cgtcatgata accttcttca gccgcccaag 132300 acacgccttt ttcactgaac gttctaaact cttgaatagt tgcgcggtga tgaataataa 132360 gcttatcttt ttcaataagg tcttttaatg tagagcatcc tactgcttta gtacgttttg 132420 tctgtttcat tcctaaatca gtatatgaat cgcaaataac gccttcgtat tctaaatcca 132480 tataaagtga tttcgcaact gacacaccag tactatttaa ttcaatataa actgggcatt 132540 cattgtattc tactaaataa cgcataacga tgtcgggtag aattaaatga gaaatagtgt 132600 ttgagtgtaa aacaccagct tgttcccaca catcatcggt aacatcaata atatgtaaag 132660 cgtggtaatc ttgcccacga ccttctgagc agtctagagt tgcaatatat tttctatctg 132720 gttcagggct tttaaatcga tgaaaaccat gatcatctgg agttacttca atgaaatcca 132780 taacagccaa tttcatacct gaaattaatg taccagaagt cccttcaaac gccgcagtgt 132840 gttcttgacg aaattgagct aaagtagaac cattaatggt ttgtatgctc cattgccatc 132900 catcgtcaaa aatatcttca tcgttataaa gacgttcttt aactgaattc caaatagcag 132960 tgtatggttc aaaccctgat ttaccttcaa cagcagcagt ccaaatatca taaaaatgat 133020 ttaatccatt aggagtcgta gtaataataa ttttcgaacg acgaccagat gaaattaccg 133080 gctgaatagc aagccaggaa tcgtggaagt ttggaataaa cgcgcattcg tcaatataaa 133140 tcattgcgaa cgagttacca cgaactgcgt caggagagga agcataggct ccaattgagg 133200 aaccattatc aagttcaata gaccctttat tccattcaac aattcctggc tgtaaaaagt 133260 caggaagcag ttcaattgct tgcttagtac gatctaaaac ttccgcagac attgagcctt 133320 tgtgcgcaag aatacctaca gctttatcct tattaaagca tacaaagtgt gcaagaaaaa 133380 tagctactac agttgtttta ccaagctgac gtgataaatt acaaacagtc atacgtttag 133440 atgacattat tttgagcata tcacgctgat agtcacgtaa ttgaaccttt atgacaccat 133500 agtcaatatg tgtaatggcg cagtatgttt ctgcaaaata cacaatatcg tctcggcatt 133560 ttttccattc ctcaaccatt tcacgagtcc actgtgtttt aatattagct cgtttcaagt 133620 taggaagacc catataccga gatcttttat tattcttatc tttataagtt tgaaataatt 133680 caggcttatc agaattattt ggaattttta ctattttgtg tagacgaaga taatcactga 133740 atttttcagg ataccatttt ccatcccact gtgattttat ccaatgaatt ccatcttcat 133800 cttttctttc tgctaagctc gggtgtttta ttaaaatttt tccagcttcg tttaacggat 133860 ggaaatcatt taatgcatta atcggttgtt ccatttatca ccttctcacg agcttcttga 133920 gcctcgtaag catcaccaat ttcgtccatt aattctgttg gtgaacccat gaatactgtc 133980 gcattctgaa tattcatttg acctgtagga acagcgcctt tggtgccaac ctgctcagat 134040 gtaatatctt tcatatcttt gtgaagcttc agtatttctc tgttcgtcgt agtcatttgc 134100 cccataagag ttgcaaatac ttccatgtga cgaggagaat cagcattctt tgccgtctca 134160 agaaaaatct tggctgcgtc cattagcatt tgttgttgaa aatgcatatt tcgacgaact 134220 actccataat catcttctaa gtcaggagta cggttttgag gattgctttt aacttctact 134280 aattgcagag gttcatatac tttaatttcc tccccgccaa ttccggggag gtcagaaata 134340 tctaaaagtt tgtttatatc aagaccttcc ataataacct ctatgttctt gggccaggag 134400 gttctggcgg tgttggtcta tttacattgc tggtgaaagt ttgttttact gttccatccc 134460 agtcttctgg gttaatatct cgaggaacaa cttcgctatc tacagattca aaaacacctt 134520 cgccatcagg caaatctctt gtattggcgt gaaaatctgt ataagtagta cgaattaatc 134580 cttctgcatc atctactgga ggatacatcc atccatttac ttcaaatgtt agtgaccatt 134640 caattctacg acgagataaa ttatctccat ctatagcttc atctatagca gcagacatca 134700 gtacaatttt aatatccctt ttaaatggaa tatcatttcc aaactgttcg tacatagttg 134760 tattaaaatg aggttgaaaa tatggaagaa tctgttcaac tatttgaaac atatcgtctt 134820 cgtatcgagt aaagatactc aattcataaa tcattttaat aggagatgga ttatactgtg 134880 atactacaga agttgcgcct ttttgcagta aattctgatt taaaatgttt gttttaaatg 134940 gagcattata gctaaaatca actaaatgta aatttatacg aggtagaata gtttcaactt 135000 tagctacatc ttcttgtgaa tttatcgatg tccatttatt taatttcatc atgaagtgtt 135060 cctttgatgc atacgtaata ggaacacgta taaacttatc accagattct aactgacgtt 135120 tgatttggat atttgaaaac aaatcgccca tcaaggtagc atatcgtcta aaagacgaat 135180 tataaaaata accaaacatg atttctccta atctgggctt taattagttt ataatattta 135240 ttaatccatg aaatcattat caaatgggct agattcaaaa gatttgcctc tgttattgac 135300 aacaacataa ggttcaacat attctttagc ttcagaatta atttgatcta cttcagcata 135360 ctgatcaata ttaatatcat gaatgccatt gagattgcga acaggattta attctaattc 135420 actaaattct ggaatgttaa ttccttcatt tttctgtaga actggattaa tttcttctcc 135480 agaataaatg aatttacctg ctgtaatttt acgaatagca ttttggccta attgataaaa 135540 tggatcatat ggttcaaccc agttaatttc gaataagctg ttatccatag gaaaatatat 135600 caaatcgcct tccttaggtt cttttccatt tacttgatgt ttaaacaagt ttggattaat 135660 ggataaagta acttcatcct gtacttgcat accaaaatta ctaaagaacg atttagctcc 135720 ttcatatcct tcgaatgaat ttaaatatgc agcgaatttc caagctttag taaatttatt 135780 ttttaagtct tcgccgaata tcaaatcagg ggaaacatac tctcttggaa cataatagca 135840 ttctacacct cgcatttgaa tgctttcagc cactaataca tcagctaata tttggctgtt 135900 tttataatga ttgaaattta cataaggatt tagtatttca gtttcattag tctgagaata 135960 accagtgcga ttttctaatt tagcaaaaag atttttatca taagtagcca tattaaccta 136020 ccaaaattcc aaatggagga tcgagcaagt ataattcttc gcgtaatgct tctttttcta 136080 atcgagcttc ttctattaag cgctgtccat caattgtgac accgccagga agcatcatgc 136140 cttggtgacg tgctaaaatt tggccattta attctttagc taaagctgta gcatagtctt 136200 tcacccaacg attattataa gcaccttgct taacatttgg gtcttgacca acaacgcgac 136260 caactaaatt gtggtctggg ttattatatc gttcagataa tgaccagcta tcttgtggac 136320 cgactgttcc atatcctact gtatttccaa ccattttatt agtatcaata tatgatttag 136380 tccagctttc tacgatgatt aaatcgtatt tttggaagtt tcccatgact ttgagttgtt 136440 catttgctga attaaaccaa aaatctggaa taggcgaaag catatcttgc atcattccca 136500 tgtaactggt aagctgggta aaatacccta aatcagctcc aaaggcattt ggtccataaa 136560 atctattaca agaagtcccc atgccaccat taataccagc cattcctaaa agaaagtcag 136620 taaaccatgg atatgtagcg tttccgtcca ttgacgttat tgatccaaca ttcgtgcgaa 136680 gaatgcgagt tactgcgaat acatttgaac ctcttaaatc gaagactcca gtcttatatt 136740 tttcttcgtc gtctcctaca taaaatacat gaaaaccttt gttaagtcca tcaaaatggt 136800 attcaccgta taattctagg gcacgctgga tgcaatcgta gatttgatcg ggtgttaact 136860 caacattaat aattggagcc cctaaacgtc ttagaatgac atctttgagt tcctttggat 136920 tctgagaatt atatcctgac atttaaaaat cctttggggc cttgcggccc catgttatgc 136980 tggtgataaa aaggtagaaa gtaataccca ttcgccgtct ttacgaacgt aagcttgacc 137040 atcttttgga gcttcaggaa tataacctgc ttcttgtaga gcttgaacat caccttgcag 137100 agaagatata ctacctttgg ctgtattcac ttcttgtgta actgatgcaa tgttggtttc 137160 gttagctttt attgaattgg ttaatccacg ttcttcgaca gttgaaccgt ttggattagt 137220 tccatttact aaagtattta acgctacaac ttgcccttta attcctgtgc tgttattacc 137280 aatctctact tgtaggttct gaacatcgtt attcaaacct gaaacagaag tttcaattgt 137340 agaaactcgg tttaaaagag acccaggagg agaaggttgt ccaccgctag aatcagttcc 137400 aacaatttgg tttaaccacg aaacattcgc tcttaaacca gatgaagtgt tttcaccgac 137460 gattccattt agagcttcga ttgaagttgt attatctttg atttgacctt taatactcga 137520 aggaatatca tctgaaccaa tagaagtttc aataacggtt aaacgctgtt taatacctcc 137580 actttgattt agctctaaat taatagatgc aatattatca gtgtttgtat taacactcgt 137640 aataatcgaa ttatttcctg gatatcctat tgaggtctta atagcactaa tgtcagattc 137700 aactgctgtc tgtttagtgt caatttcatt taagcgactg taaacagttt gtgaaaacga 137760 tggtggtttt ggtccaagct cttcacggag attaccaact tcaatagtca aagaacctac 137820 atcagattca ataaattttg tttctaattc gcttaaacgt attccttgtg aagtaatgac 137880 atcagtatta ttaataatgc gatgcttcat tcctgtgcta ggatttccta caacaggaag 137940 accattaata tcttggcctg tatattgtcc aagttcacgc tttatccaca gtaaatcatt 138000 tctgatcgtt ctgtatacag agttggcctc ggagttaaat ggaccaatat cagcaagaat 138060 gctgtcaacc gtactattag tcccatttaa tatttctgta tgttcactgg ttagcgtttt 138120 aaggtcagaa atatcctttt tattgatact tatttgggcc aaagcttcaa tatcaccaga 138180 tacatctaag atgccctgaa ttgttttaat atcactattc gcggattcta aagatgtttt 138240 gagtattccg atgtttttat cgagaacttc aacatttctt agtacagaaa ctgtaggtct 138300 attcattgag ccttcacttc catactgtgt gtcagctcct aatatttctt cgccgttttt 138360 aatccaggaa atgcggctct ggccctttgc aggagggccg tcaacgaatg gtaagtcatt 138420 cagtacaata tctgtcattc ttttacctta attatgtagt ttaaagaaat gttccatgga 138480 cgtgtttcat ttccaattaa ttcgggacga tttaatgtat atttggaatt tcgttgtgat 138540 gctgggtcaa tttcatatcc gtcattggtg aagtatgaac ggttatccca gtcaagtcct 138600 ttacgtgtac caacaaaatt tgatctacgg gtattaccga atgctccaga atcatcatgc 138660 tcaccaaatc cgccagcgtg tttatgataa gacatctgct gtttttgtac ttcgccaaca 138720 tatccaccgg tacaacctac acctaatcta ggcttaccaa attggtcatt accattaacg 138780 tttggatttg ttaagtgaga gccacgacca gagccacgaa caaaaagacc acgcatatca 138840 ggtaatccag gatttgatga gcttccgccg tatcttgttc caattctaga agcatataac 138900 ggacaatctg atgctgaaac agttccacca tgacagaagc gccaagcatc actaggaaga 138960 ctatctgccg cccacatcat aatagctccg acaggaatag ttctatctat ttcattctgt 139020 gttacaacac gcttaccttg aatataacca ccagtcatgt taactgttcc ggtaatattt 139080 gctccacctg aagctattgt aagcgtatta ttaatgcgaa gtgttccatt aatcgtttga 139140 ccacctcttt ggtggataac atcagcattc caagctagtg ctgatgaagc atcacctcca 139200 ctctgtgagc cggcggttgt agttaattta actacgcctc tcattgacgt tgtagaacca 139260 cgaccattaa gagttgcgcc agtaactgca acagaagcat tattcgagtt aacttctgat 139320 tgtgttccta atttaattac gcctttatat tcttcagtag cagtagaatt cattaacgta 139380 taaggagaaa ttgcataacc ttctcttaat gttccttgac gaacttgagc tactgtcgct 139440 aattgaacaa caccttggtc cgattcggta gctgtggttt cagaaggagc gatttgcgca 139500 attaatttaa tagctaactg ttgtgttttt aatggagtca tcgcagtagt atcatctgcg 139560 ccagctaatg cttgtggtag agatgaaatt ttaataactc catttgatga ttcagttgaa 139620 actcgtgttt caaaggcatt attaagggca acagtaaatt tagcgggagt tatagaagat 139680 tcattattaa ctccggcaat tgcttcatca ttggttgaat atcttgttaa tccgtaaaca 139740 gtttcagttg cgtttggata agataatctt gttgccaacg ttgctggtgt aactgctttg 139800 gtattatcag ttccgtcgat aacttcctgt tcagtggcaa gaaataaaat tccttttgtt 139860 gttgatgatg catcaggaac tccatttaca ccagcaggag aaatggctgc tatagcagcc 139920 tgaacatcag taatctctgc cggaaaattt gtaccagttg gatcaaattt tacatagcga 139980 gattcattag aaacgtgttg atatgtatta ttactcatgc tgttctctca aaatgataaa 140040 gtactattgg agtttgctga ttatctaaag ttacagtttg tgcaccaagc aaattccatg 140100 tgccatatcc aggcttagat tctgaaataa tttcttggga aatagttatt ccatatgtag 140160 aatatgtgct taatacgtgt ttttgattat ctaaataagt aacttctaat ttactaccat 140220 cggttggatg gtctttatat gaattgatag cagtaaatga cgcaatagct tcttgtagcg 140280 caacgcgaac tttggcaaca aactcgatag ctgtcattcc ggttgttgct gaaaccggaa 140340 gaccaaatac taatacagta acaggagaac caggaggaag cgaatcatct gtcacagttc 140400 ctgaaaatgc ccaataatca gtttgagaaa caccctcagg agaaacgccg gttttattag 140460 taataattgt accaacgtct tttatagaaa aattatgaac atcattaata gctgattcta 140520 cgtttggttc ataaaatcct ttcgctaatt gagaaattgt cactgaccca actgattgac 140580 gattcattac atcgatgtcg ccagttttag gtctaaaacc aagaaaatcg gctaagcggg 140640 aaataactcc cgctttatta ttaagtaaac tcattatgca atccttatcc aacgataaac 140700 tgtaatatat ggttgaatgt tagttattga tgttggcgga gtgtgagtag agttagtaga 140760 tgctttagct tcacggtatt tcgtgtaaac tggaccagat tcatctggat cgtattgaca 140820 tccaccaaca atgactgatc cattttcatc aactattaga acttcttcat ctgtctcagt 140880 tgcaggaaga ttagcatttt ccaatgtaac agaagtagaa ccacctgttc cgcctgcagt 140940 atgtgaagga tttccacccg aatctaaatc gttgttattt agagcaaagt tagggtccga 141000 aatatcttca ttccatccaa ctaaaacttt tccttgccca aataatttcc atgagccgaa 141060 tcccatatat gtaacagggt tatttggatt aacagcgttt tcatatatag ttccaatagg 141120 ataaatcgta tcaaagatat ttgaaacagc attaaattca cgttgatagg attgaactgc 141180 ttctacattt ggccaaccaa ttttatcgaa gtctgttaaa tttacatcac cggaaatatc 141240 tactcctggt ccttgcgata catagagatt atcagtttca tcaataatct catcttttgt 141300 taataatgta cctaaatcat tattaaacca ggtgatatta ataatgtcgc catgctcaaa 141360 tactctatcg aagtgcaatg tttctggtat gccgttttca tcagtttcaa ctgaatagtc 141420 agtatgagac tgttcccaag ttccacctaa aacagagcat tctacttcat cgtctgaatc 141480 ggcaccaaca caatgaaata atggcattcc aactgttcca gccaattctt gtaaaattcc 141540 attaaaacgg acctcgagag aattaggatt aataggttca cctggaatta atccaaaagc 141600 tgaaaacgga attgatttca ttgttgataa atcagtaacg taaatgcttc cttctaaaga 141660 agtctttgac gttaattttg aatctaacaa tctaatttga cgtcttgtat atgaacttct 141720 ccactgtgat acgccatcca taaatgtttc aatttgaaca gtatcgccaa tattacaagg 141780 ctgtcttaat cgaatattaa atccatcaag aggaactaat tcgccttcat tttcgcccgg 141840 agagccaaaa tcgctgtttt cactaaatac atcgccgtaa tacaattcgt taccacgatg 141900 ttttactctg atgttattta cattataact agttccacgg aaaacatcta aaaagtctgt 141960 ctgtccttgg acttcaacta aaaattcttt acgagctaca ttactaatgt ctgaactggt 142020 aattttgtca atttgcttat ttttgacata ttcccaacgt cctggggcgc aatacactaa 142080 ttccaaatcg ctgaattgaa cattaatttc aactggtact gcagaccctt taatcgtatc 142140 gccggaagca gctactaatg taactgggtt gacgttccat gtagcaaata cgtctctagc 142200 tctaattacc ttgttgtaat cattgacagt tccttttgga agatttatag ttactcttcc 142260 agaagaggta ttaatagcgt atgattttcc ccactcagct tttagtgttt ggccagatga 142320 agcatcataa gttttccagg caccggctga atatggaaca tctccatcac caagctcata 142380 ataaagctca tcaaagtttt catttatttt tataccacct ttacgcaggt agtcaccggt 142440 accatcatca acaacattac cgatattaat attttgtttc attattgagc caccccgatt 142500 ttctgcgtag cgataacttt aactgctgct ctcataccga cagttgcaga acttatagtc 142560 gccgttacat aattttcttt aatactaaat gcaatattag cgatttcgtc ttcttcggtt 142620 tcattcccaa cccgcatgac agcatattca gaagaaatga cctctgaatt aacagtatcc 142680 acaagaatat ttatttctgc tgttttaatt tttcttccat ctactgattg gcatgtaact 142740 agcaatttag ccatattgta ttcagtgcga tgaaatagtg gaatatcaac tgatccggat 142800 gtagaaatat tccatgtacc ttcagctggt gattcctttt gtccaaacat gctttcaata 142860 gaataattcc aaaccgatgt agaattatca gatgaaatac agcgtaaagt tactttacta 142920 tatgggctag tcactactaa attacccgaa acacctttaa ttgaatcaat cgcttgaatc 142980 gttaaaggat tagtaactga tatagatcca tttgagttaa taaattcaac gcagtcgcca 143040 agttcgcctc tttcaatgat aactttaaca cctacagtag aggtatcaat atcatgccta 143100 gtgccaactt ttactggagt tgcgtactct gcaatagagt gtttttgata atatccagta 143160 gcatggataa tttggccatc tgctccagtt ccatttgcta ctgccatttt acgttgatcg 143220 ccaaacgcat tataaattgc gttaaaatca ctattaattt tattaccacc gtcgaataag 143280 atatcaccag tagaagcgtt accaatttcg ccggtatcaa tcaatttctt tggttcttga 143340 atgaacatag cggtttcctt atgagtttat agtatttata aagaaaaagg gagcccatgg 143400 gctcccttaa gttaaaatgt aaacagaata ttgatttctt ctgtttgatc cattgccatg 143460 ataataggtg gcctattttc catataaatc atttcgcccg aatgcctcat taaatcttcc 143520 ggatcataat aatccttttc agctttaacg tttgggtcat ttggatgagc tttagcttca 143580 agaggattcg tgattattga tatttgtcta aatcctttat ttcccggcaa tgcagcctca 143640 ggaaaataaa ctgaatctaa atatgcttta aaacggatag tatttgcctt aacacggtaa 143700 attaatccaa aatcatcttg ttgccaagtg agattatctt cataccccca tctagccggg 143760 tcttctttta attcctcagg ccaaggaacc acgatatatt cattcgtgca tctatttata 143820 gatacatcag gcggaatctc aaaaagatat tcccaaacat atccgtctcc aggctcgatt 143880 gttccttccg catctcctcg accttcagga ggagtcattg acctagcgga aggagtccat 143940 tttccaccta acttaaggca ttcatcttta tcagttaaag actcaattga gcacattcca 144000 gtatcaggaa catctaaaca gcgatatact aaccagcctg cgcctgattc ggtagcattg 144060 taaggagctg agttacatac tacaatatcg ttaattctaa atgtgtatgg atccggatat 144120 ctagtatctc cccaatctcg acgaggaata actgcatcaa gcatagatgg aagaaccttt 144180 acagttccca tcatatgagt ccacatgtca gttacaccta atacagaatc ggtgggataa 144240 ggtggggcaa atcccacctc attttcattt gatgaccatg gttctgatct tccaaatgtg 144300 ataaagatag tgtttttatc cggaccactt ccaattgaat tataaaaatt caacattttt 144360 tctgttctaa attttgaagt aactatcgca cgatagataa cacttgaatc attcatctat 144420 tttaacctgt gttggatttt cagggtctct tggatttcct atattatctt ttagacgttt 144480 attaactaaa tctctaaatt gtgcaaatgt tgttccagat gcatcgaata aaggactcat 144540 taatttacgt cgttcagatg gcaattgacc ttgaaatatt gaattattat tttcagcatt 144600 atagtcatca ggaagaggat attttacacc agccattggg ccaggttcat aaattgcttc 144660 gcctgttacc gaatcatgct caatttcacc ggttggagtc aatttagcta ctctatcagc 144720 atattcagta ggcaatccag aatcccattt atagtttttg tatttattaa ttatagtctc 144780 tgtatgttta agagttaaac caacattaat aaacattgtt aaaagggtaa ttgctataaa 144840 tccaaatcct actggatgaa caaaacgaat aacgtcagat ttccagcggg aagaaggcaa 144900 attggattta attttcatta cataatatga ccgacttcta ttaatatagt ctatattatt 144960 ttgaagcaaa tccttccctt taactccacg aataatttcg ccttcaaaac taggaagtct 145020 ttctgcttta acttcttgac cggcaattaa tcttcccaaa agattatgaa tagttacggt 145080 ccattgtaat ttaccgttag aatagcttct ttctatataa gtaacattac atcttcctgt 145140 ggctgtataa atcgtttgtc ctactaaatc ttcagtcaaa gaatcggatt gaacgattat 145200 atcatattca gtaccagctc cagattcaat ttcaatttca acctcttcat tataaagaac 145260 tttaaaaaga aatttgtatg atgcttcaat tcctttagta gaataaaaat catagctacg 145320 tgattcgaaa aatcttgcaa cagcatcacg tttgtcagca tttaaataaa tgtttctttt 145380 atatatctct gaccacaaat attcccatgc atgctcttct cgtggatatt tgttacgaat 145440 taaatttcgt aaattgttat actgagttcc atatccatca gaaagatact gaatatatgc 145500 ttcgcaaaat gcctcaaaat tcgaatcctg caacaaataa gaatcaggca tcattgtgcc 145560 aatcaatggt cttaaatcag ggtcagctaa tccacgctct tcttcaggcg accagggggt 145620 ttcatgttct tggttttgta aaaatgcttt taaaaatact tcagttggct tccaaataat 145680 ttcgacagtg tctctaacac gatagttaaa atcatagtaa gaaattattt caccagaggc 145740 tttatagaaa agcatcccag atgcatattt tttaaatcca gtgaatttaa catttggcat 145800 tactatagtg caatcgccat catcccagta ttcatgaact aatctatctg gtgatgtttc 145860 cggaatattt tctataactt tagtgtattt taaatcagca taaactacta caaccctatc 145920 ggagttgttt atccaacaac gtgtgttaga ttttttagac cagttaaaga acggttctgc 145980 gtagtatttc atcggctgag gagtaaaagt ttcccaatca gacttttcat ctgctataaa 146040 tgccatcata tgataatgct tatcagctaa ccattcgcga ggaaattcat atttaacagc 146100 accgattaac tgatattttg ctgcagtttc agggtcatta acaacattat cacttaaaaa 146160 tttaaaatta ctcgatgata gagaaactaa tttaccatca gttgacatat tcgcgtaccc 146220 tggttgaata cgtcttcttt cttcttcagt gttgccaaaa actcttttcc acgtttttgt 146280 gtcgtgattt aaaacatata ttcctttatc agcagaatca attatttttg atgttctagg 146340 attagcattt aatgtttcaa cttcaccaat aataagagca aaaactttat caccgataga 146400 atccatttta tagcatactg ctttaggatt tccggttata gtcattgtat caggttcaaa 146460 aagcctttcc gaatatgttg gagataatgg atcagaatct ataggtgcat tgctcgtttt 146520 tatgtatcta actttgtctc tggcgacaac gtaaatgtaa tcatcggtac aagtaatagc 146580 ttctgcaatg cgatatacat tcgctggtaa agtcgcgtaa gtggcaaaaa tttctacatc 146640 aaatcctaaa tgtaattggt cgccaagttt agcaaatgtt atatcctgag aactaaatct 146700 gacatcatca gctgaccatc taacatcagt cgatttgcgg ccatagaaaa tcttgtcgta 146760 tcctagaacg tatgttgtgt tcgcagattg gtaatatact gtcttagata aaggatatcc 146820 tacgcggtca ttgaagagct ttacagcttt ccaagtttgt cctttatcat tggatacttt 146880 aactacaggt tgataacgct caaaaaggta taaaatccct tctgattcca tcaaataaac 146940 tcggttaata tccttacata cttgctgaat agaaccttgt atttcatgat actcattttc 147000 accaataata aaattgctta ttgatgaaac atcaacatat gatggactga attgaaaaga 147060 ttcattcatc aatgcagcca ttatagtgtc attattaaaa ttaacataat cagaattatt 147120 aagagtaaat ttttcctgaa tgaatttatt ggctaattgc atttcaatca tgttttgaaa 147180 tgtataagca tttgtagcaa aagtttcaaa ttcttcggta taaatccaat cagactgctc 147240 aaaatcttgt gcagctgtag ctactctaat aacgtatgat gtcaatgggt cagcatcatc 147300 aaaaaagaag ctgttatttg cagtatatcc taagttaatc catctgtatt gattactcgg 147360 aagattttcc cccgagtttg tttttgtctc agcgatttct acaaaatagt agaaattagc 147420 accaacgtca tcccagcgta cttgcacctg atttgcggat aacttggaaa ttctgagact 147480 agtgactgaa ggtgctttta ctgtcattgt gatataggct ccaaatcgat agttaagtat 147540 tgtggacgta agtcattttc aaatacaatc agtgaaccgt cgcgagtaaa aataacatca 147600 tcggttgggt cagaatataa ttcgatggtc tgaacttcaa atttttcaga tgttaaatta 147660 attttagcga tattccaata aatcacatca gctggataat ttatttcacc gataacatag 147720 tatttgtcgc gtccatcaga atttgctaat ttgttaaaat cgttgcctgt atatggctga 147780 atgttttcat tttctgtaac atcgccagaa gcaaatggac caataataac tttaccaatt 147840 cctttagaat ctcggtctgt tgatactatg cgaacgtcat ataatacatc ttcttctaaa 147900 ccagtatcag gatttacaac ctttcgtcca gaattaaatg aaaacgtatt agattccata 147960 gaacgatctt ttatttgatt attgtattta atacctgctt caggcgtttt atagaagttt 148020 tgtacttcac gaacaatttg aattgtcgct gatgaaccaa tgatagaatg atctgcatca 148080 tctacatatg tcaacatctt agatttagcg aaagatgagt taaaaatttc tacatcttcg 148140 gtataataac gatcaatttt atcaattatt tgaccttcga gccactgttc ggattcttgc 148200 agcttattta aagcatatgt gacttttaaa ttagtcttaa taaaaagata attaggagaa 148260 ataattgatg gcgtaatagg agctaaatta tagtctttga gataattttt aatatcttcg 148320 cgctgtacgg tagttaaata cagacctgat ttaggtttag cagcaataaa tgcataacca 148380 ggtttagtag aatcagtgaa cgtctgaact gcttgaataa tagaaccaaa tctctctgaa 148440 acgaatgtat catagtcagt cgcagttacg cagcgttgct gggtttcgcg tttaatagta 148500 cccaattcgc gaatacgttc aatatcttct ggatcgccgc cgccatctgc cccaacaaaa 148560 tctgggtcat cgtttggatt ttcattaatg ttgatgacag ttatatttgt taatgtatct 148620 gcatatgaaa atccgactgc accattcgca tctgcaccgt tagtactgat gtactcaata 148680 acaatcgtag agttctgggt aggcttaaga cctccaatat aattagcagt caatgctcct 148740 tctgccgcat taacagaaat ctcgccttca ccaaaataaa actcagtatt tccatcaata 148800 gtttcacgca tgtagtaaat tgtcgatgta gaaccagcat gaaccattga ctttcttgtc 148860 cagttaatcc attctgctcc atcaacatat agtttaactt ggtttctatc tatatttttg 148920 tcataaatga taataggtgt taatttatca taaatgattt cagttcttac tatacgtccc 148980 tgggccaatt ttaaacgtgg aaaatattgg ttatttttat cgcgaatagc aataacatct 149040 tcggtagata caaagttgta aggattaacg gaagtatcct ttgcatatgc taaaaagcga 149100 gtaccacgag gaatagtaat gtaatttcta ttcaatgcgt ctgtacaagt tagcataatt 149160 tcagtttgag cagctgattt tgaagtaggt aaatatccat tatcttgtgc agcttgaact 149220 actgaactac gcaaattagc agtacgcata aagctttcat acaaggcagc attaccaaac 149280 tgctgaatgt ataatgtatt ataagccaaa aggtcgcaca gaacgtttaa tcttgagcct 149340 tcaaaatcat aatccaaaaa ttcattttgg ccattaagcc attcaatgag gttttgtttt 149400 atttcagcaa atgtaccccc gacgaatatc tcgggaatag catttgctgt tcttgttaat 149460 tgataattta caggggtatt tgccatttta aatcctattt aatgaatact ttagatgatg 149520 cctgagccac tgtatcaccg catgatattg gatcagccat ttgaacagct ttctttccag 149580 tgacatatac cttagaagtt ctaggttgtg tcactccgcc atgtgtttca tatggctttt 149640 taatttctgt atgttctgta attggatcac ctgctacgag aacagcaatt cctccagtga 149700 atactttact ttgcgtagca ttcacaactg ttggaggcca tgcttcatgg ccggcagtaa 149760 cacacttatc ataacttaat cctgacattt atggcctctc atacacgtag cttctcaatt 149820 tattagccca acggctccaa tttccaacta tagtttttgt ataattttta acaagagttt 149880 ttcttattgg agctggagga tcagttggtt cagtagtatc agaagatgac ctagaattac 149940 tgccagaacc tccagattca ccttgttctt ggtagtcata tattaatgtt acttcgtaag 150000 tgaatgtctt ctggaggttt tgaggagctt tccataaata taactgagta ttagaatcag 150060 ttggaaggtc ttcccatgaa gcagcagttt taaattcatt atctaaacga tatttcaacg 150120 catcatttcc aaatccaaat acagattcat acgttccata taaacgattt tcttctacta 150180 aaaccccagg agtttcttcg taactagtta tatttataga tactaacgtt tcacctgttt 150240 ctaattgagc ggtaaaggtg acgtcgatag aagaaccttc catggattct cctaaatcag 150300 cgctcattgg aagtatatta gccaatgtca atcctcgatc catcaattgt gtattgacca 150360 gatgaaatag aactcataga tgccattttt tctgtccaat caccaccaac gtcccaatca 150420 actgtcccag caactttcca agaaagattt ccatttactg tattagtctg atttccttca 150480 actaaagtgg tagcatctcc tttaactgta atgtcagcat taccttcaac tacaatagta 150540 acattacctt tgactaggat agttccatta ccttcaaccg tcttagtttc atcgccacgc 150600 acaaatattg tattgcttcc atctatttgg tggagacgat tatccatgtt gtaataaatt 150660 tctgacccac cgacgttagt ctttttatcg ccagctacca aaaaattacc atcagcattt 150720 gttatatcat acaaattatc aacagttttt cttgttcttc ttcctgacgg tgatacttcc 150780 tcatatgttc cagtcggatg aactaatctg tatcgttctt gcccaggggt atcatcaaat 150840 tcctgaatat gaccgctttc agtttccatt gtgtgaacat acggatattc gcctttatat 150900 gaagaaacag gttctttaaa taaaattctc gaatcattcg gaataggagg gtcagctgga 150960 tcagaagact ttgctacagc agcagccatt gctgacaaag acctagccgg tgttttcact 151020 tcaataccgt atgattccaa attacccgta agaataatca tggtaacacg ggatgcacgg 151080 ccttttgttt gttgatacca caatgaatca cgaccagctt tatatgcttt ttcccaatct 151140 ccggctaaca tagcagttaa cattgtgtta aatttagcta caccgccaac acccatctga 151200 aatgccatat tttctaatgc catttgacga gaacgattaa ctgcctgcca aactgggcca 151260 actttagaat gtgatttaat gtcacgttgc atatcagcca aatcacgttc aaataaagtt 151320 accgcttctt ccattgtaat agaacctgga tttccggtaa tttcacgacc aacttgtttt 151380 gataaaactt tattaatttg agtcatatca cgaactggct gcttcatgat aagatgacca 151440 ataccaattg tcggatatcc ttcagtatcc caataaactt ttagtcttaa tccttcatca 151500 cggcgaagca tttcagccat tgacatattt ggattatcat cagttggaat ttctgacagc 151560 ggtctatcat caggatttat cgcagtgtct agattactgt cttggataat attagaagac 151620 gaatcatatc ctacttctcc gccttggttt aatacgttag tatcatttcc tagacgtcta 151680 ggatactgtc cagtcgggtc agaaaatcct tcaagtctat taggcttttc gcgaactatt 151740 ccaccatacg tgccaaggac aattccgtta gttttccatt tgtctaaaaa atgaccataa 151800 actctagttc cttctactgg accagtaacg gagcctccaa ttccagacat tgctgcagaa 151860 gttataggtt gaataactga catccatggt aatttatcag ttggaatacc cattacgtcg 151920 ccttgtgctc tttgaggtgg atgcagacca actacacgaa cacgaacacg acctaatttt 151980 aatgggtcca ttctatcttc aacaacacca acaaaccaat taaggttatt acttatcatt 152040 tccataagat ttctccatta tacgtataag gtcgttcata aatgaattga tgtctgattt 152100 tgctattatt tttatttgac gaagtttttc attttcaaga acagcagctt catacgtatc 152160 gaccgcagca agtgctcctt catattgagg atattttcta gctttatcgc ctttgtcata 152220 ccaaacatat ggattatcat cgtatgatat taaattataa aatttttcac cgttctcatt 152280 cacatgatat actatttggt ctccacctac atttttgtat ttttgtatag atgcttgata 152340 agcagcttct tgcgaagtaa tccatccata atacggatca taattatcat tacacatcaa 152400 taaaacccaa tacaactgcg gatttccata tatagcattt gctaattctt ccgggcgtgg 152460 tgaacctttg atatagtaag tgcgtaagcg atatcccgca agagcgcgtt tgaaatagtc 152520 tttatagttt ctaaaaatat ctgtcatagg aatagtcggc gcgtttttat tcaccgtttt 152580 ggccgcatat tcaatcggat caaaaaatgt aaagagcatg ggccctcctg tttataaata 152640 tattatctat ttataaggag aatccaatgg catattctgg aaaatgggtt cctaaaaata 152700 tatcaaaata tagaggtgac cctaaaaaaa ttacgtatag atcaaattgg gaaaaattct 152760 tttttgaatg gttagataaa aatccagaaa ttattgcatg gggtagtgaa acagcagtaa 152820 ttccttattt ttgtaatgca gaagggaaaa aacgtagata cttcatggat atttggatga 152880 aagattcttc tggtcaagaa ttttttattg aaataaaacc taaaaaagaa acacaaccac 152940 cagttaaacc agcacatcta acaactgcag cgaagaaaag atttatgaat gaaatttata 153000 catattccgt taatactgat aaatggaaag cagcgcaagc tttagctgaa aagcgcggaa 153060 taaaatttag aattctaaca gaagatggat tacgagctct tggctttaag ggggcataat 153120 ggctattttt caaataatta atgaaagcac tccccaagtt ccaaaggtta agcaatcatt 153180 aaacgaaaag aaatggattc agataggtct tgaatacaaa aaggccaaag caaaaggaat 153240 gacaggaaag caatttgctg aagaaagagg aatcaaatac tctacgttta cttcagcaat 153300 gtcaaaatat gcttcaggaa ttaaaacagc tgaaaagatt caaaagcttg aatcaaaacc 153360 aatgaataaa ctcaataagc aagaaagaca actgcttatg ataaattcat tcaggcaaac 153420 gttgcgtgat aaaattcgta atgaaggtgc agcaattaat aataaaacca gaaagtggtt 153480 tgctgaaact attaagcagg taaaaggaca taaagttgtt cgcccgcagc cgggacgaat 153540 atatgctttt gcttatgatg ctaaacacaa ggaaactctt ccttattggg ataaatttcc 153600 tttgataatt tacctcggtt taggtaaaca caatttaatg tacggattga acttgcacta 153660 tattccacct aaagctcgtc agcaatttct agaagagctt ttaaagcaat attcaaatac 153720 acctactatt actaataaaa cgaaattaaa aattgattgg agtcaagtga aaggatttag 153780 gggtgcagac caaatgatta aggcatatat acctggtaat attatgggta gccctgttga 153840 aatcgccccg aaagactggg cgaacgttgt gttaatgcca ctccaaagct tctactccaa 153900 aggaaaacgt ttctctgcaa acaaagtttg gagcaatgta cgatgaatta tgaaaaaatt 153960 tataattcac taattgatag ggctcgcaat agagccctta ctggatataa agaaagacat 154020 catattattc cgaaatgttt aggcggtagt aatgatgctt ctaacttggt tgatttaact 154080 ccagaagagc attatgttgc acatcagcta ttagtaaaaa tacacccaaa ttctttaaaa 154140 ttatcttatg cagccataaa aatgacggtt tcaactaaat atgttaggag aaataataaa 154200 gtttatggtt ggttaaaaag aaaacatgca aataatattt caaatacaca aactggacga 154260 aaatattata ataatggtaa aagatgtata aaattacatg atggagatat aattcctggg 154320 ggatttattt taggaagagg atggagtccg acacaaggaa caactcattc tagaaaaaat 154380 gacacgttta aaaacaaaaa gcttcaatct gctttagcta ataaacggtg ggaaaaagaa 154440 aagaaaataa tgttagataa ctttggagtt aacactattg atgaagcaaa gaaaattgtt 154500 cttgatttta aatcaacttg tcatccaaga tattggcgaa agccaacttt agaaaaattt 154560 ccatttatga cttctgctcg tttaagaagt ctaataaatt aattctatta tcttccattc 154620 ttttctattg tttgttctaa ttggaattga atggaaggga cttagaccca ttataccacc 154680 aacatttata aagcattatg aggaatatat gtcgcaagca ctacaacaaa tttttaacca 154740 agcaaataca actaactttg tagtatcaat accacatagt aatactacat ctgcttttac 154800 tttaaatgct cagtcagttc ctattccggg aattaggata cctgttactg ataccgtgac 154860 tgggccgttt ggactaggcc gagcacagcg tccaggagtt acatttgagt atgatccact 154920 catcgtgaga tttatagttg atgaagagct taagtcgtgg ataggaatgt atgaatggat 154980 gctaggaact agtaactatc ttacaggcga aaatactgcc caaaaaacag gtcctgaata 155040 cattacgctt tacattttag ataacagtaa aactgaaatc gtgatgtcaa taaattttta 155100 taagccttgg gtttctgacc tgtctgaagt agaatttagc tacacggaag attcagaccc 155160 ggctttagta tgtacagcaa cgattcctta tacgtatttt caagtagaaa aagatggcaa 155220 aattatagca gaagtttaat gcttcagttt catgtgttat aatcttaact aaatttgagg 155280 agaaacatat gaaactaatc tttttaagtg gtgtaaagcg tagtggaaaa gatactactg 155340 cagattttat tatgaataat tattctgcag ttaaatatca acttgctggt cctattaaag 155400 atgcattggc ttatgcatgg ggtgtatttg cagcaaacac tgactatcct tgtttaactc 155460 gtaaagagtt tgaaggaatt gactatgatc gtgaaactaa tttaaatctg actaaattag 155520 aagtaattac gattatggaa caagcatttt gctatcttaa tagtaaaagc ccaattaaag 155580 gtgtgtttgt ttttgatgac gcagttaatt tcgtagcatt taacaagatt gctgacgtta 155640 taaataatat tgaagatcaa tggtcagtcc gtcgtctgat gcaagcccta ggtacggatt 155700 tgattgttaa taacttcgac cgcatgtatt gggtaaaatt atttgcttta gattatcttg 155760 ataaatttaa ctcaggttat gattattata tcgttcctga tactcgtcaa gatcatgaaa 155820 tggatgcggc tagggcgatg ggtgctacag taattcatgt agttcgtcct ggtcaaaaat 155880 ccaatgatac acatattaca gaagctggat tgccaattcg tgatggcgat ttagtaatta 155940 caaacgatgg ttctcttgaa gaactttttt ctaaaattaa aaatacacta aaggtactat 156000 aatgtctgaa caaactattg aacaaaaact gtctgctgaa atcgtaactc tgaagtctcg 156060 tattcttgat acgcaggacc aggcggctcg tctgatggaa gaatccaaaa ttctacaagg 156120 aactttggct gaaattgctc gtgcagtagg tatcactggc gatactatca aagttgaaga 156180 aatcgttgaa gctgtcaaga atcttactgc tgaatctact gatgaagcaa aagatgaaga 156240 ataatggaat ttaaagactt ttcaacgggt ctttatgtag cagctaagtt ttcagaatta 156300 acacttgatg cgctggaaga actccagcgc tctttacgtg ttcctaatcc agttcctaga 156360 gaaaaaattc attcgaccat atgttattca agagtaaatg ttccgtatgt tccatcaagc 156420 ggaagttttg aagtagcttc ttctggacat ttagaagtat ggaaaacaca agatggatcg 156480 actcttgtac ttgtgctaga ttctgaatat ctgcgctgcc gacacatgta tgcgcgggca 156540 ctaggtgcta cacatgattt tgatgattac acaccgcata taacattgtc ttataatgtt 156600 gggcccttat catttagcgg tgatgtacaa attccggtcg tattagatcg tgaatacaaa 156660 gagcctctta aactcgattg ggcagatgat ttaaaataat ttcacaaagt tgtttacatg 156720 ctgatgaggt agtgatacta ttatctcatc aaaattaatt aggaaaataa aatgaaaact 156780 ttcaaagagt ttgctacaaa aaccactatc actgaatctt ctcacggtat ggaagtaaag 156840 cttggaatgg ctttagctga agctgagcgt cttttctctc gtattaaaga acttgctgct 156900 gcagtcgatc cttcatcttt taaaggagac caaactaaag ttaaagcact tttagcgtta 156960 tgctccgatg caggagaaat tgctaaaaat ggttctaaga tgaagaaacg attagaagat 157020 ttaaaataat ttcacaaagt tgtttacaca gggttttagt agtgatacta ttaccctatc 157080 aaaacaaaat caaatggaaa tcaaaatgaa aactttcaaa gagtttatcg ctgaatcttc 157140 tgatgtaaaa gttgagttta tttacactgg caagaaagat aaaatgggtg aaatgcctta 157200 tggagttctt cgtgatgcat tagataattt cggtcaactc gccgcagaag attacggtga 157260 taaaattgtt gttactggtc ctgctgcagt tattgaaaaa tgggcagcag aaaataaatc 157320 aatttttcgt aaaaaataag tttactttta gatggggtag tgatactatt atctcatcaa 157380 ctactgagga gaataaaatg aaacgttgtg aattaattcg aaatgttgct attgcaattt 157440 ctgcttctgc ttttagtttt tcaatgtttg ttggatttat atgcggatta ttgactacag 157500 cagaaaatgt tttttcactt gtagtagcat ttttaattgg tttaattgct atcgttatgg 157560 ataaaatttc taaaggtgaa tagaatgtac aaagtgtatg cggattatca ggctaatcca 157620 tctgaaaagc caggttgtca aattggatgg gctcatgata ctttgcttga agcagtagat 157680 gctgctcaaa aattgggata taaatatgta gagattgttc aaccgtcagg aaaagttatt 157740 actcttgaag agttcaataa cattaaacca aactttttac tttttgcagg tgataattat 157800 tatcctcgtg gtggatatgc tgatttaata gcgaaagcca ccactgaaga tgaactccgt 157860 gacattatca aagaaaatga aaacaaacca gattatggtt ctaatcgctt tgattggtgg 157920 caaattgtaa atgccaatac gcatactatt gttgatgaag gttgataatg attctttatg 157980 cgaaagtatc atccattgaa aatggatata aatatgatca agacgcggct aaagccttga 158040 ttgatgatta tggcatttta acatattttg aagttgaaaa ggtttacatt gaccgttcat 158100 cttcacaagt taaattagtg aaggaagacc gtaaatttaa tacagtaaat tttgatttct 158160 ttatcgaaac agaaaaaggt cctcttgaat atgatatttt caagaatcct ttgggtcttg 158220 aatgtattaa atatacttac attaatgcag cgtggtgaaa atgaatattt gtttaggtac 158280 tacaattccg cagggctacg taattgatgt cactacctgg gaaaatgatg gtgacaacta 158340 taaaaccaaa acattgtttg gcgtagaaga gcatgaaatc ttacaatttc agtatctttt 158400 aaagaagttt aaaagcagat attctagtac taaaattgag cgctattgtg gtaatggttt 158460 gttcagcgag caatctcttt ttatttatga atacttggtt gaaggactgt tctcagatca 158520 gctttatcca gaattcatta aaaaggtctt tcgcatagaa gttgatcttg gtaataaatc 158580 tgaagaagat gaagaacgtg tatttgactt attctttgtg aatggtaata aagtgcttga 158640 aggacttatt aatattttag gtaatgcttc tgagtattat gattatgatt ttttgcgtgt 158700 agttgagagt gttaaatact cttatataga agaagaaatt gttttgccga ccgttaaaat 158760 gtttaatttg ctttaagaat tatttgttat tattaactca taccgcactg attaataccc 158820 tctatcatca agggttcttg cttgagagcc tttgttaata attgggaatt agccaagttg 158880 gtaaggcact ggattttgat tccaggatgc aaaggttcga gtcctttatt cccagcgcga 158940 gaatggtcaa attggtaaag gcacagcact taaaatgctg cggaatgatt tccttgtggg 159000 ttcgagtccc acttctcgta ccaaagtttg cggatatcgt ataatggtat tacctcagac 159060 ttccaatctg atgatgtgag ttcgattctc attatccgct caaatttaat ttactccgtg 159120 tagctcagtt tggtagagcg tctgctttgg gagcagaatg tcgtaggttc aaatcctgcc 159180 gcggagactg gaggcgtggc agagtggttt aatgcaccgg tcttgaaaac cggcagtcgc 159240 tccggcgact cataggttca aatcctatcg cctccgtaat ttcgctgatt tagctcagta 159300 ggtagagcac ctcacttgta atgaggacgt cggcggttcg attccgtcaa tcagcatatc 159360 aaggccctgt agctggaagg ttcaagcaag cgactcataa tcgccagatg gtggttcaat 159420 tccacccagg gccatatttt gagaacataa atactcttag aaataggaga tatttatgtt 159480 ccattacatt tataaaataa caaataaaat taataataaa atctatatag gcgctcattc 159540 taccgaaaat ttagatgatg gatatatggg ttctggcaaa ttattaaaaa gagcccaaga 159600 caagtatggt atagaaaatt tctctaaaga aatattagag ttctttgacg ataaaacctc 159660 tatgtttgag tcagaaaaga atattgtgac tgaagagttt ttaaagcgcc ctgatgttta 159720 taatttgaaa ttaggtggtg aaggtggttg ggaccattta aacactaaag aatatttgga 159780 taaaaagaaa gaagcttcct taaaaggagc tgccgctttt aaaaccagat ttgagaacga 159840 cccagacctt aaagaaaaat atcgtaaaat gggttcagaa aggtttaaac agctttggga 159900 taatcctaag tatagagaga aatttttaaa tactaaatct ttcttgggta agacccatac 159960 tcctgaaact attaataaaa tgaaagaatc tcacgccaga aataaacacc aacagggcga 160020 aaagaattcc caatttggta tgatgtggat tcattcatta gatgaaaaag tttctaaacg 160080 tattaagaaa actgacccaa ttccagaagg atggtttaaa ggtcgaaaaa tgaaatttta 160140 attacggggc atagctcaat tgtatagagc aacggacttc taatccgtag gttgaaggtt 160200 agaatccttc tgtctcgacc aaacaagaaa ccgggtcgct accggtaagt cgtcggactg 160260 atgttccctg agtaaggaat tgcgttaata atctttacgt ttattgatgc ccccttatat 160320 cacagcagaa acggcgcacc gaattatcga ttcgaggaaa tatctttgcc gtaagccgag 160380 tagcgttttt gacggaacgt tcggatacgg tcgagatatg gccttttaaa aaatattgag 160440 tagcgtcaac tgcttaataa ccgggttcga atcccggcgt ttcgtacaaa cacttgcctt 160500 agcaggtgga accccgacaa ggttgccgca aggcttagcc ccgaccgaaa ggttggggct 160560 ttttagtata aatatagtag tatattaaat ccacgaatta aaacaggaaa taagatgaaa 160620 tcatatgctc aatttttaaa tgaagcggtg ttaaatgaag catctagcac cgaaattcaa 160680 gctgtcgcaa aagctgccat tgccgcgggt aaatattcct ataaagatgc ttccgatgaa 160740 tcacgattcc aatttgctcg tgatatgaaa gccgaaggat ttacagggaa tgcagttagt 160800 atggcttgga aaagtttagt tgctactggc gctgcttttg caaaggcttc gggtaaacct 160860 gctcctaaag cagatcctaa agtcgcacaa gagaaaaata ttgttaaagg aattatctcc 160920 aaatatgaag ctatccttaa agagctttta gtaatcaaaa ctgaaggcca aaaactggct 160980 cgtgcttata gtttcaaaga taacccacat gctcactctc ttgagtatgt tgaagacatc 161040 caaaaaatta ttaaagaccg catttggtct gctaaacaaa tcaaataaca ttcttagccc 161100 tagccgaaag gttggggctt tttggtatct agacctttcc aggcctctct agacatcatt 161160 tagtttctac cctttataat atattatcct atcctttgat ttcccctccc tgccctagaa 161220 ttccccaaaa ctttttaaaa attttttcac aaaactgttt acatccttgt ccttccatgg 161280 tactatacaa ctatcaacta ctgatacaga aaacaacttg gagaatgaaa tggataatta 161340 cggtgaactg ttcaacttct ttatgaaatg tgtttcagaa gattttggtc gtacagtgaa 161400 tgatattaaa gttatcggtc ctgatcatcc gatgtttgaa acttacgcag taatgggtaa 161460 tgaagatggt cagtggtata ctgtaaaggt tgtgattaac atgttcacag cagaaggtta 161520 tgttaaactg tcttctaaag tttaccacga taacgacgaa atcgcagaag aatatttcaa 161580 taatatgaaa taagtttaca caggctcatg attgagatat tatgagcctg taacttgagg 161640 aaaactgtta tgtctcttta taccgaacta aatgaaactt ccgaaaaatc ttttgatggt 161700 atcttatcaa taatgcaaaa gcaggtatta gaacaactta gtgaagccgc aagcaatggg 161760 cacaaggctt gtcgagtcca aagcccacat cgtcgcatgt atgaacgccc tcttattaat 161820 tggttagaaa gcgaaggtct tacggttaag atttgccctg ctaaatttga tgtcccacgt 161880 attcttaata tttactggga taaaccggtt gataaaccgg ttgagaaaag tgacccttgg 161940 gttgttaatg taaaaacatg tgataatgga tgctcacttg aggtgtctgc tgtaattaaa 162000 ggcactcacg gtgaaaaaac gtggggttgg cctggtaaag ataaagaaat cgtgttaagt 162060 gtaaaagcta ctagtattag tactcctata gctaaaagca ttatcactgc cgctgttgaa 162120 gaagctgaac gaatttgtaa aattaaaaat agtttacatc tcttaagtag catgatataa 162180 tttttctgta aattttaatt tctaatcaac ctaaggaaat actatgaaca cactgaagaa 162240 aattgttgag tttattcgca ctaaacttgg ttctgctatg gctaaaaatc tatctgttga 162300 agagcagtat actgctgcgg cagcaaaact acttgataaa attaaagacc taaaaactgc 162360 ttctattaaa tctattaatg aagaaaaacg tattcgtgaa cttattgtcg aaaagaataa 162420 gcaggccgaa tcaaaagagc gtgaaattcg caagcttctt tccgaaggtc aagatgtaac 162480 aatgcatgct aaacttggtt tactatatcg tcgaacagct gagcagttga ctactaaggc 162540 tgacggttat gccgaaatgc gaattgaaat cgctaagaaa gtagttgagt tagatgatgc 162600 tcgccaagaa cttgcagtta aattggaata tatccgtgaa actcgtgcgg caaatgccct 162660 tggaattagt actgctgatg atgtagttga aattgcagca ctgactaagg ttgatattga 162720 agataccctt gctcgagttg aaacctttaa cggtaatatt tctggggttg aaaccacctc 162780 tgccgatgtc caggaatata ttaattctct gaaataatga taaggggctt ccgcccctat 162840 tacttggagt aaataggaat gaaaatgcaa agtgatttca attcaatgtt tgaagagttc 162900 caaagacagg ttgatgttcc agatcaatta ctaaatgctc ttaaacgtat ggcagaagga 162960 cgtaattact attgggggtc ttcatacgaa actgatgaaa gtctttctgg attttataaa 163020 ggtaaaaagt ctttaatacg tcctggaata ctcattaaaa gtattgaatc aattcattca 163080 ttgacgtgtg attttgatgt tgaatttact gatttcattt ctcctgaatg gacagtttgt 163140 tatttaaatg acgattatga ttatcttggc gtttacagtt taagtgacgc atggtttaaa 163200 cgtaatttac aaaagtcaaa tttgttctat attgatacta cggtaaaatt tcagggaaag 163260 aaatatttct ttactcttat agttgactct gaaacgaagc atgaaaataa acgtattctt 163320 agtaaaagaa atatcttgaa tattgttgat gatctttttg ataaattagt ggaaaatcct 163380 aattttgaaa gtgatttatt actagaaaaa tttgttaagg aatgtagaga atatgtcaaa 163440 gccatcacta taccttccaa gtaaaccttt gaagtatgaa ttaaagcgcc agctaattcc 163500 tactgatgta ttaataggtc ctgttatact catatcattt gtaattctat tgattattgg 163560 aggtgtttta gatgttatga ctgatattga ttctggcgaa atacttgtgt taatgctaat 163620 tcttccatta atagttccac ttttattagt acctgtaaat tgggtaggat actggtatca 163680 aggaaggcat tatcgtaaac gcgtacgtga ttggaaagca cagtgtaaaa agattaaaaa 163740 ggaacatcag cttaaacttg ctgcgtatga atttgatgaa attatgaaat ttgttaagga 163800 atcacgatgc aaaagccaaa actaaataaa gtcaaatatt cgcttcctga gtcattttta 163860 atttttgctg tggcgttggc tgcagctctt gcgggtagtc tttttggatt attaattgac 163920 tgtcttattt taaacatcga cggcacagta aatataatag aagtttggag tgaacttcgc 163980 tatactaaaa ttatttcatt attttcattc tttggtacca tgctatattt tcattatgat 164040 aattttaaaa taaattggca aagaaaaaag gattacaaaa tacaattaaa agaatataat 164100 agctacatgt cttatattga aaatgaatca atgaaagagt ttgtgagtga ttgcaggaaa 164160 atcaaatgat tttaaaaact cgctggtatg atttagatga cggggatgat ggcatttcag 164220 ttgatagagt tgactggaat ggctgttccg aagatatgaa aaatttgctg attagggagt 164280 ttagaatggg atatcaacca gttaagccat ctactgcaac agatgataaa ttcgtgtgta 164340 ttcataatgg tcgcgctaaa ttaacgaacg ctgattggtt cacagataag attatgattc 164400 tgtggtatat tattagtttt cctgtgacat catttgtatt ttgcttcttt ggagaaagtc 164460 cagtaggcag aacggaagat tggattcttt taattatact tgttaatatt tttacagcag 164520 cagtactatc aggaatatgg tgcgtgttca ttgaaatgcc atggcggcta cgcagacaac 164580 aaaagatttt tgatgaaaag aaatacgctc aaaatttaaa taactttatc actgaatgca 164640 ggaaattaaa atgaaaacac tatcagctgg tattatcttt atgacagaag ataaagattt 164700 atttatgggt cgggttactg gttctcgtaa gcctggaatg atggcacatc gctgggatat 164760 tccaaagggg cgtgtagaaa gttctgattt gaatgcactg gaagctgcaa aaagagaatg 164820 tttagaagag accggtttta gcaattataa tccagacctt ctagaagacc taggtatatt 164880 taaatattct agtaataaag acttacagtt attttattat acgattccag tagagcatga 164940 gatgttcaga aattgccatt gcgagtctta ttttgaaaat aaagatggcg ttatgattcc 165000 agagatggat gcttttgctc ttattcctcg tactcagtgg caatatgtga tgggtccttc 165060 actttaccga ataatgaaca acctctttta attttataaa taccttctat aaacacttag 165120 gaggtattat gaatatattt gaaatgttac gtatagatga aggtcttaga cttaaaatct 165180 ataaagacac agaaggctat tacactattg gcattggtca tttgcttact aaaagtccat 165240 cacttaatgt tgctaaatct gaattagata aagctattgg gcgtaattgc aatggtgtaa 165300 ttacaaaaga tgaggctgaa aaactcttta atcaggatgt tgatgctgct gttcgtggaa 165360 ttttgagaaa tgctaaatta aaaccagttt atgattctct tgatgctgtt cgtcgttgtg 165420 cattgattaa tatggttttc caaatgggag aaaccggtgt ggcaggattc actaactctt 165480 tgcgtatgct tcaacaaaaa cgctgggatg aagcagcagt taacttagct aaaagtagat 165540 ggtataatca aacacctaac cgcgcaaaac gagttatcgc tacgtttaga actggcactt 165600 gggacgcgta taaaaatcta taaagctgtt tactttctac tagaattgtg atagtatatt 165660 cacagttgct tgaagagaca aattactaat taaaatattt aaaggaaaca tatgaaaaca 165720 tatcaagaat ttatcactga agcagttatt aattctcaaa ttattgctga atcttttact 165780 gatcttttga aatttaaaaa aggtcaaaaa attactgctg tattagatga tggtacagaa 165840 gttgagatgg atgtacaggg atataattat gcagtagatg gaaaactgta taataaatct 165900 catgctaaat ttgattcatt tgacgacttt gttaatacag ttgaagatga aaaaactcgt 165960 cgatccattg caactggtga tgctaaggtt cttatggcgc acggtcatga acgtatccgc 166020 gccaaacaga ataaaatggg cgaagataat tttgcattag ttggttatca atctggtaaa 166080 caaacttatg gttatcagcg tactgctacc atgtataaca aaaatggtaa aattgccttt 166140 gtaaatagta aaggttctat tcagtacgtt aaatcgttca aataatatgg gaacagcctg 166200 gacctcattt gaggtattcc cgccaacctg tgataaggtc gagccaaagc gcggtaatgg 166260 gtaatgcagg aatggacaac tcatgtgcca tggaatggcc caaaaattaa gagagaaaat 166320 atgagaacat ttttaactgg cccgtatcta tccctgatga atgcttttac acaccattct 166380 gatgctagag tagaagaact ctgtaagaat gattatcttc caccaatgga agatgtgctc 166440 aaacagtact gcacacttcg actagatggt ggacgtcaat ctggtaaatc aactgctgtg 166500 actaactttg ctgctaattg gctgtatgac ggtggaacag ttattgttct ttctaatact 166560 tcagcttacg ctaaaatttc tgcaggccac attaaaaaag aattttctcg ttattctagt 166620 gatgatattc gattccgctt atttactgat tctgtccgta gttttatcgg taataaagga 166680 agcaagttca gaggtttatc gctttctcga attttgtata taattgatga gcctgttaaa 166740 tctcctgata tggataagat ttatagcgtc catattgaca ctgtacacta ctgctgtaat 166800 agtaaatgtt gcattggtgg tattactcgt ccgcagtttt tcgtaatcgg aatgcaatga 166860 tgacagacac acagcttttc gaatatcttt atttttcgcc aaaaactatt aaaaataaat 166920 tggtgaatca ttttgaaatt ttggcaaata acaacgtctt gagcgaattt tatcctaagc 166980 aatacaaatt acaaaaaggt gtattcaaag gatgcagagt tttgtgtact gctcctaatg 167040 caagattaat gaataaaatt ccatatttta ccatggaatt tattgatgga ccttttaaag 167100 gactaatcac ccaaagttta atggcatatg attctgagcc atttttaatt aaagaacagt 167160 cttggataaa tttattttct aattgaggtt atatgaaagc atatcaaatt cttgaaggca 167220 cacataaagg tactatttat tttgaagatg gtattcaagc acgaattatt gtttctaaaa 167280 cctttaaaga ggactctttt gtagacccag aaattttcta cggtttgcat gcccgtgaaa 167340 ttgaaattga accacagcct acagttaaaa ttgaaggtgg tcaacacctg aacgttaacg 167400 ttctacgtcg tgaaactctg gaagatgcag ttaggcagcc 167440

Claims (8)

  1. 서열번호 1의 염기서열로 표시되는 박테리오파지(Bacteriophage) KFS-EC3(수탁번호: KCTC 14585BP).
  2. 제1항의 박테리오파지(Bacteriophage) KFS-EC3(수탁번호: KCTC 14585BP)를 유효성분으로 포함하는 항균 조성물에서,
    상기 박테리오파지는 병원성 대장균(Escherichia coli), 장염균(Salmonella enteritidis), 살모넬라 미션(Salmonella mission), 살모넬라 센프텐버그(Salmonella senftenberg) 및 시겔라 손네이(Shigella sonnei)로 이루어진 군에서 선택되는 어느 하나 이상의 병원성 세균에 대해 사멸능을 갖는 것을 특징으로 하는, 항균 조성물.
  3. 제2항에 있어서,
    상기 박테리오파지는 접종 후 26시간동안 용균활성이 지속되는 것을 특징으로 하는 것인, 박테리오파지 및 이를 포함하는 항균 조성물.
  4. 제2항에 있어서,
    상기 박테리오파지는 -70˚C 내지 60˚C에서 용균활성을 갖는 것을 특징으로 하는 것인, 박테리오파지 및 이를 포함하는 항균 조성물.
  5. 제2항에 있어서,
    상기 박테리오파지는 pH 3.0 내지 pH 11.0의 범위에서 용균 활성을 갖는 것을 특징으로 하는 것인, 박테리오파지 및 이를 포함하는 항균 조성물.
  6. 제2항의 조성물을 유효성분으로 포함하는 항균 소독제.
  7. 제2항의 조성물을 유효성분으로 포함하는 항균 세척제.
  8. 제2항의 조성물을 유효성분으로 포함하는 항균 사료 첨가제.
KR1020210072550A 2021-06-04 2021-06-04 병원성 대장균, 살모넬라 또는 시겔라에 대해 사멸능을 갖는 박테리오파지 및 이를 함유하는 항균 조성물 KR102592424B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020210072550A KR102592424B1 (ko) 2021-06-04 2021-06-04 병원성 대장균, 살모넬라 또는 시겔라에 대해 사멸능을 갖는 박테리오파지 및 이를 함유하는 항균 조성물

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020210072550A KR102592424B1 (ko) 2021-06-04 2021-06-04 병원성 대장균, 살모넬라 또는 시겔라에 대해 사멸능을 갖는 박테리오파지 및 이를 함유하는 항균 조성물

Publications (2)

Publication Number Publication Date
KR20220164159A KR20220164159A (ko) 2022-12-13
KR102592424B1 true KR102592424B1 (ko) 2023-10-25

Family

ID=84439131

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020210072550A KR102592424B1 (ko) 2021-06-04 2021-06-04 병원성 대장균, 살모넬라 또는 시겔라에 대해 사멸능을 갖는 박테리오파지 및 이를 함유하는 항균 조성물

Country Status (1)

Country Link
KR (1) KR102592424B1 (ko)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100958139B1 (ko) 2008-03-31 2010-05-18 주식회사 인트론바이오테크놀로지 엔테로코쿠스 패칼리스 특이적 사멸능을 갖는 신규한박테리오파지
KR101260655B1 (ko) * 2010-06-09 2013-05-10 주식회사 인트론바이오테크놀로지 살모넬라 콜레라수이스 또는 살모넬라 두블린 감염을 방지 및 처치하는 방법
US9402873B2 (en) * 2011-09-09 2016-08-02 Intron Biotechnology, Inc. Method for preventing and treating Salmonella Typhimurium infection
KR101581654B1 (ko) * 2013-06-27 2015-12-31 주식회사 인트론바이오테크놀로지 K99형 대장균 감염을 방지 및 처치하는 방법
KR102068591B1 (ko) * 2018-05-03 2020-01-21 경북대학교 산학협력단 병원성 그람음성균에 항균 활성을 갖는 박테리오파지 유래 재조합 단백질

Also Published As

Publication number Publication date
KR20220164159A (ko) 2022-12-13

Similar Documents

Publication Publication Date Title
KR101592177B1 (ko) 대장균에 대한 광범위 항균 활성을 갖는 박테리오파지를 활용한 대장균 감염을 방지 및 처치하는 방법
AU754264B2 (en) Chlamydia trachomatis genomic sequence and polypeptides, fragments thereof and uses thereof, in particular for the diagnosis, prevention and treatment of infection
RU2725726C2 (ru) Бактериофаги, фаговые пептиды и способы их применения
AU745787B2 (en) Enterococcus faecalis polynucleotides and polypeptides
KR101649851B1 (ko) 신규한 시가독소생산 F18형 대장균 박테리오파지 Ｅｓｃ-ＣＯＰ-1 및 이의 시가독소생산 F18형 대장균 증식 억제 용도
AU2015205512B2 (en) Phage therapy of E coli infections
KR101761581B1 (ko) 신규한 장침입성 대장균 박테리오파지 Ｅｓｃ-ＣＯＰ-4 및 이의 장침입성 대장균 증식 억제 용도
KR101986442B1 (ko) 류머티스성 관절염용 바이오마커 및 이의 용도
KR102191537B1 (ko) 포유류에서 골 소실을 예방하는 젖산균의 선별 및 이의 용도
KR101993123B1 (ko) 신규한 병원성 대장균 특이 박테리오파지 eco5 및 이를 포함하는 항균 조성물
KR102592424B1 (ko) 병원성 대장균, 살모넬라 또는 시겔라에 대해 사멸능을 갖는 박테리오파지 및 이를 함유하는 항균 조성물
KR101859974B1 (ko) 신규한 에로모나스 하이드로필라 박테리오파지 Aer-HYP-2 및 이의 에로모나스 하이드로필라 균 증식 억제 용도
KR101992013B1 (ko) 병원성 장내세균에 대해 사멸능을 갖는 신규 박테리오파지 및 이의 용도
RU2790834C1 (ru) Композиция антибактериальная для санации лабораторных приматов
KR102203675B1 (ko) 신규한 여시니아균 특이 박테리오파지 ye12 및 이를 포함하는 항균 조성물
KR102418861B1 (ko) 포도상구균에 대한 사멸능을 갖는 박테리오파지
KR101993124B1 (ko) 신규한 살모넬라균 특이 박테리오파지 sg1 및 이를 포함하는 항균 조성물
AU2021240230B2 (en) Vaccines and vaccine components for inhibition of microbial cells
KR20230172913A (ko) 병원성 대장균 또는 시겔라 속 균의 특이적 제어를 위해 축산 농가로부터 분리한 신규 박테리오파지
KR20180122992A (ko) 신규한 비브리오 파라헤몰리티쿠스 박테리오파지 Ｖｉｂ-ＰＡＰ-3 및 이의 비브리오 파라헤몰리티쿠스 균 증식 억제 용도
KR20180061778A (ko) 신규한 비브리오 파라헤몰리티쿠스 박테리오파지 Ｖｉｂ-ＰＡＰ-3 및 이의 비브리오 파라헤몰리티쿠스 균 증식 억제 용도
AU1546202A (en) Enterococcus faecalis polynucleotides and polypeptides

Legal Events

Date Code Title Description
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right