CN113528463A - 一株裂解性、高效价副溶血弧菌噬菌体rdp-vp-21010及其应用 - Google Patents

一株裂解性、高效价副溶血弧菌噬菌体rdp-vp-21010及其应用 Download PDF

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CN113528463A
CN113528463A CN202110894035.4A CN202110894035A CN113528463A CN 113528463 A CN113528463 A CN 113528463A CN 202110894035 A CN202110894035 A CN 202110894035A CN 113528463 A CN113528463 A CN 113528463A
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vibrio parahaemolyticus
phage
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杜新永
崔耐
王晓铃
李先胜
马如霞
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Abstract

本发明公开了一株裂解性、高效价副溶血弧菌噬菌体RDP‑VP‑21010及其应用，其宿主为副溶血弧菌，透过电镜观察，该噬菌体头部呈正六边形，头部直径约为56nm，尾部长度为103nm，为长尾科噬菌体，基因组全长为12558bp，保藏编号为CGMCC No.22481。副溶血弧菌噬菌体RDP‑VP‑21010对水体中副溶血弧菌具有强裂解作用，具有较高的温度耐受性和较宽的酸碱耐受范围，可以高效治疗该病原菌在水产养殖中所引起的副溶血弧菌疾病，以及可以作为消毒剂，为工业化、规模化、程序化生产噬菌体及治疗副溶血弧菌引起的水产养殖细菌病提供了噬菌体来源。

Description

一株裂解性、高效价副溶血弧菌噬菌体RDP-VP-21010及其 应用
技术领域
本发明涉及生物技术领域，特别是涉及一株裂解性、高效价副溶血弧菌菌噬菌体及其在水产养殖中副溶血弧菌感染中的应用。
背景技术
副溶血弧菌又名致病性嗜盐菌，是一种海洋致病菌，为人畜共患病原菌。在1950年在日本的爆发性食物中毒中分离发现。副溶血弧菌分布极广，主要分布在海水和水产品中，据统计，我国华东地区沿岸海水中副溶血性弧菌的检出率是47.5％～66.5％，海产鱼虾的平均带菌率是45.6％～48.7％，在夏季时可高达90％以上。除了海产品以外，在畜禽肉、咸菜、咸蛋、淡水鱼等都发现有副溶血性弧菌的存在。
近年来，我国的水产养殖业获得迅猛发展。随着海水养殖规模的日益扩大和集约化养殖方式的不断推广，鱼虾疾病的频繁发生对水产品的规模化养殖的健康发展造成了严重的威胁。水产动物的发病要素主要有细菌、病毒，寄生虫等，其中细菌病是对水产动物度危害程度较为严重的疾病。目前，对于副溶血性弧菌的预防和控制的主要方法是使用抗生素，虽然抗生素使用方便，广谱抑菌，见效快，但是，抗生素的滥用也导致了细菌的严重耐药性，在副溶血性弧菌所造成的疾病防治中的效果愈发下降，因此，急需开发新型抗副溶血性弧菌的药物。
噬菌体是一种细菌病毒，在自然界中具有较高的丰度，具有宿主专一性强，不会造成微生态环境的破坏。噬菌体侵染宿主菌后，裂解时会释放出新的噬菌体，而少量的噬菌体就能起到防治的效果。副溶血性弧菌噬菌体的作用靶点在原核微生物的细胞壁，而对于动植物细胞上没有相应的作用位点，增加了噬菌体使用的安全性。并且噬菌体和病原菌可以共同进化，防止细菌耐药性的出现。因此，利用噬菌体来解决细菌具有广阔前景的生物防治途径。
本发明在于响应国家全面禁抗的号令，解决细菌耐药性，抗生素滥用的问题，提供一株治疗副溶血弧菌的且安全可靠的裂解性副溶血弧菌噬菌体，扩充了噬菌体储备库为开发治疗副溶血弧菌的抗生素替代品奠定了基础。
发明内容
本发明的目的在于提供一种专一性裂解性副溶血性弧菌噬菌体RDP-VP-21010，以替代抗生素在防治水产养殖中的应用，并解决水产动物发病急而治疗不及时，且因致病菌耐药性而导致用药无效等问题。
为实现上述目的，本发明采用如下技术方案：一株裂解性副溶血性弧菌噬菌体RDP-VP-21010，透过电镜观察该噬菌体头部呈正六边形，头部直径约为56nm，尾部长度为103nm，保藏编号为CGMCC No.22481。
本发明利用双层琼脂平板法从养殖淤泥中分离到一株副溶血弧菌噬菌体，其基因组全长为50642bp，该噬菌体在pH为5-10中作用6小时，其效价较高，可在2.2×1010pfu/mL之上，具有明显的嗜碱性。在pH为12时，效价可达到106pfu/mL，在pH为4时，效价仍可达到107pfu/mL，可见该噬菌体的酸碱耐受范围比较宽。该噬菌体的最适温度在37℃，效价能达到1.7×1012pfu/mL，该噬菌体在﹣80℃的条件下可长期保存，效价维持稳定。该噬菌体可特异性地裂解本实验室从青岛即墨养殖户取得的患病虾病灶处分离的致病性副溶血性弧菌BV-21002，其最佳感染复数为1:1。将该噬菌体命名为副溶血性弧菌噬菌体RDP-VP-21010，该噬菌体于2021年06月03日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为：北京市朝阳区北辰西路1号院3号，保藏编号CGMCC No.22481。
本发明还提供了所述的副溶血性弧菌噬菌体RDP-VP-21010在制备抗副溶血弧菌制剂中的应用。
本发明还提供了所述的副溶血性弧菌噬菌体RDP-VP-21010在制备抗副溶血弧菌BV-21002药物中的应用。
本发明的另一目的在于提供该副溶血性弧菌噬菌体RDP-VP-21010在制备预防和或治疗副溶血弧菌引起的疾病的药物中的用途。
本发明的另一目的在于提供该副溶血性弧菌噬菌体RDP-VP-21010在制备预防和或治疗副溶血性弧菌引起的疾病的饲料中的用途。
本发明的另一个目的是提供一种用于防治副溶血弧菌感染的杀菌组合物，包括有效量的副溶血性弧菌噬菌RDP-VP-21010。
本发明的另一目的在于提供一种副溶血弧菌噬菌体的应用，该目的是通过将副溶血性弧菌噬菌RDP-VP-21010用作为由副溶血弧菌引起的细菌感染提供潜在的治疗药物或保健品来实现的。
本发明的另一目的在于提供一种预防和或治疗副溶血弧菌引起的疾病的添加剂，该目的是通过其有效成分包括副溶血性弧菌噬菌RDP-VP-21010来实现的。
本发明还提供了一种清洁剂或消毒剂，包括副溶血性弧菌噬菌RDP-VP-21010。
本发明的有益效果体现在：
(1)本发明发现并分离了一株致病性副溶血性弧菌BV-21002，并以该致病性副溶血性弧菌BV-21002为宿主分离得到了副溶血弧菌噬菌体RDP-VP-21010，该噬菌体RDP-VP-21010对水产养殖环境中致病性副溶血性弧菌BV-21002具有强裂解效用，为工业化生产噬菌体用于水产养殖环境中致病性副溶血性弧菌BV-21002的防治提供了噬菌体来源；
(2)本发明所提供的副溶血弧菌噬菌体RDP-VP-21010是从自然界中分离的烈性噬菌体供试噬菌体不含毒力基因或不良基因；本发明未对供试噬菌体进行任何遗传修饰；
(3)本发明提供的副溶血性弧菌噬菌体RDP-VP-21010，当水体中加入副溶血弧菌噬菌体RDP-VP-21010后，8小时内有明显的裂解效果，具有较强的裂解活性，表现出了良好的应用前景。
(4)本发明提供的副溶血性弧菌噬菌体RDP-VP-21010，在pH为5-10中作用6小时，其效价较高，可在2.2×1010pfu/mL之上，具有明显的嗜碱性。在pH为12时，效价可达到106pfu/mL，在pH为4时，效价仍可达到107pfu/mL，可见该噬菌体的酸碱耐受范围比较宽，表现出了较高的温度耐受性和较宽的酸碱耐受范围，效价较高，安全有效；
(5)本发明提供的副溶血性弧菌噬菌体RDP-VP-21010在防控水产动物致病性副溶血性弧菌中具有良好的应用前景。
(6)本发明所提供的副溶血性弧菌噬菌体RDP-VP-21010可作为应用于环境消毒的各种产品的有效成分。
(7)本发明副溶血性弧菌噬菌体RDP-VP-21010可作为应用于环境消毒的各种产品的有效成分，例如包括但不限于以液体浸泡、喷洒、与含水性载体联合使用等形式对配水系统、医疗设施、养殖业设施、公共及私人设施或其他环境表面进行消毒去污，可有效控制目标细菌的生长及活性。所述液体浸泡、喷洒形式包括但不限于洗涤剂、消毒剂、去污剂等；所述含水性载体包括但不限于磷酸盐缓冲液、LB培养基、氯游离水等。
(6)本发明的副溶血性弧菌噬菌体RDP-VP-21010，可以有效杀灭海产表面的副溶血性弧菌并防治海产在保存过程中副溶血性弧菌的污染，可作为海产食品防护的各种产品的有效成分。本发明包括但不限于以液体浸泡、喷洒、与合成组分联合使用等形式对由副溶血弧菌侵染所导致的食品腐坏进行预防，尤其适用于熟食或不宜灭菌的食品。所述液体浸泡、喷洒形式包括但不限于食品除菌剂、食品消毒剂、食品防腐剂等；本发明中合成组分包括但不限于苯甲酸、苯甲酸钠、山梨酸、山梨酸钾、丙酸钙等。
(7)本发明的副溶血性弧菌噬菌体RDP-VP-21010，可用于制备组合物，试剂，或试剂盒；可应用于副溶血性弧菌的快速检测，其包括但不限于以试纸、试剂盒等形式对目标样本中的副溶血性弧菌进行检测，或对临床样本中的目标致病菌进行筛选，可有效确保检测的灵敏度；
(8)本发明的副溶血性弧菌噬菌体RDP-VP-21010可以单独或混合使用，用作为生物杀菌剂，饲料添加剂，或因副溶血性弧菌引起的细菌感染的治疗药物、保健品及医疗器械；副溶血性弧菌噬菌体RDP-VP-21010可应用于治疗或预防由副溶血弧菌引起的感染性疾病；
(9)本发明的副溶血性弧菌噬菌体RDP-VP-21010可以应用于工业生产，可由宿主菌特异性扩增，可应用标准病毒纯化方法高度纯化，具有极好的推广前景。本领域技术人员可以根据本发明的记载及本领域常识将本发明所述副溶血性弧菌噬菌体RDP-VP-21010或其组合物制备成应用于医疗，检测，消毒及食品防护等方面的各种产品加以工业应用。产品形式可包括但不限于以载体携带、浓缩注射或药剂浸泡等形式施用于被防治的寄主体表、口部、直肠、胸膜内部等部位；作为实施方案之一，所述载体携带形式包括但不限于口服含水性载体、口服无水性载体、乳膏制剂等；浓缩注射形式包括但不限于疫苗注射、胸膜腔注射、经脉注射等；药剂浸泡形式包括但不限于气雾剂、漂洗剂等。
附图说明
本发明上述的以及其他的特征、性质和优势将通过下面结合附图和实施例的描述而变得更加明显：
图1为本发明噬菌体RDP-VP-21010的电镜图；
图2为本发明噬菌体RDP-VP-21010的酸碱稳定性示意图；
图3为本发明噬菌体RDP-VP-21010对宿主菌BV-21002的裂解曲线图；
图4为本发明噬菌体RDP-VP-21010热稳定性示意图；
图5为本发明噬菌体RDP-VP-21010遗传稳定示意图；
图6为本发明噬菌体RDP-VP-21010D的RTD实验示意图
具体实施方式
下面结合附图和实施例对本发明做进一步说明。以下实施例将有助于本领域的技术人员进一步理解本发明，但不以任何形式限制本发明。应当指出的是，对本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进。这些都属于本发明的保护范围。
本发明使用从青岛即墨一养殖场的患病虾病灶处分离副溶血弧菌BV-21002，并通过实验确定了BV-21002的致病性和耐药性，以BV-21002为宿主，从养殖淤泥中分离得到一株裂解性副溶血弧菌噬菌体，并通过全基因组序列确定了RDP-VP-21010的基因型。
实施例1病原菌及噬菌体的分离及鉴定
(1)致病性副溶血弧菌BV-21002的分离及其鉴定
从发病对虾的鳃取样，采用无菌操作的方法，在TCBS培养基上涂布，37℃培养18-24h后，在TCBS琼脂平板上呈现边缘整齐的圆形绿色菌落，直径约2～3mm；然后挑取典型菌落在科玛嘉弧菌显色培养基上划线，37℃培养18-24h后，在科玛嘉弧菌显色培养基上呈现紫色菌落，挑取典型菌落继续划线纯化3次，然后挑取单菌落接种于5mL的2216E液体培养基中，37℃，200rpm振荡培养6h，得到均匀浑浊的细菌悬浮液。再通过16sRNA分子鉴定，确定为副溶血弧菌，将其中一株命名为BV-21002，保存于﹣80℃冰箱。
(2)噬菌体分离及鉴定
本发明试验用淤泥样品为2021年从青岛即墨养殖户水底淤泥中采集，作为噬菌体分离的样品。
样品处理：取适量淤泥浸泡于20mL 2216E液体中，10000rpm离心10分钟，除去较大杂质及大部分细菌后，再用0.22μm微孔滤膜过滤除菌。
制备混合菌悬液：取2mL滤液和1mL宿主菌菌悬液，一起加入27mL高压灭菌后的2216E液体培养基中，然后置37℃培养箱中，过夜培养。培养后取3mL，10000rpm离心5分钟，再用0.22μm微孔滤器过滤除菌，收集滤液备用，滤液即为拟含有噬菌体的原液。
噬菌体分离：采用双平板法进行噬菌体的分离，取混合菌悬液滤出液0.1mL和副溶血弧菌菌悬液0.1mL混合均匀后，加入加热至45-54℃3mL4％的2216E琼脂中，然后铺双平板，放于37℃培养箱培养6-8h后，观察结果，如果有噬菌体，那么平板上会有透明的噬菌斑。挑取噬菌斑于1mL3％盐水中浸泡10min后，取0.1mL浸出液和0.1mL菌悬液，加入加热至45-54℃3mL的2216E琼脂中，铺双平板，37℃培养箱培养4-6h纯化，照此步骤纯化2-3次，至噬菌斑大小均匀为止，由此得到一株噬菌体，并将其命名为RDP-VP-21010。RDP-VP-21010在双平板形成的噬菌斑直径在4mm-5mm，圆形透明，有晕圈。
(3)菌体效价的测定
将纯化好的噬菌体铺双层平板，然后取单个噬菌斑，加入2216E液体培养基中，同时加入宿主菌悬液，将噬菌体增殖后，再将增殖液10000rpm离心5分钟后再用0.22μm微孔过滤器过滤，将滤液做10倍梯度稀释至10-8倍，取最后3个稀释度的噬菌体稀释液0.1mL和宿主菌悬液0.1mL，铺双层平板，测定噬菌体效价。噬菌体效价(pfu/mL)＝噬菌斑数*稀释倍数÷0.1。结果表明，其噬菌斑形态、大小和清晰度一致，有典型的裂解性噬菌体特征；该噬菌体的效价可达10^12pfu/mL以上。
(4)噬菌体的保藏
将噬菌体增殖液与60％的甘油按照1：1的比例混合后，于液氮中保存。
实施例2噬菌体的电镜观察
取20μL含噬菌体粗制颗粒的液体滴于铜网上，自然沉淀15min，并用滤纸从侧面吸去多余液体，加一滴2％的磷钨酸(PTA)于铜网上对噬菌体染色10min，然后用滤纸从侧面吸去染色液，待样品干燥后用电子显微镜观察噬菌体形态。
由图1可知，该噬菌体RDP-VP-21010为长尾科噬菌体，头部呈正六边形，头部直径约为56nm，尾部长度为103nm。
实施例3噬菌体基因组测序
通过对单株噬菌体的富集培养后，在4℃条件下8000g离心15分钟，加入10％PEG8000和0.5M NaCl静置过夜，再加入等量氯仿混匀，静置分层后5000g下离心10min，去除氯仿层和PEG层后，加入限制性核酸内切酶消化处理，并采用梯度密度氯化铯条件下悬浮噬菌体，后期采用TM缓冲液透析3次，每次30min，送华大基因生物测序公司进行全基因组测序。结果如序列表所示。
实施例4：噬菌体pH稳定性的测定
用稀盐酸和稀NaOH溶液调节生理盐水的酸碱度，配成pH值为2、3、4、5、6、7、8、9、10、11、12的缓冲液，再用配置好的缓冲溶液将噬菌体稀释成1×1010pfu/mL，将稀释液在37℃下，水浴1h，用生理盐水10倍比稀释后铺双平板；37℃倒置培养4-6h后计数。
由图2可知，在不同pH条件下培养1h后，噬菌体RDP-VP-21010在pH4.0-11.0范围能维持较好的活性，噬菌体滴度没有明显变化，而且在pH为8.0时，噬菌体效价最高，达5×109pfu/ml。随着pH值的升高或降低，噬菌体的滴度下降明显。上述结果表明，噬菌体RDP-VP-21010能耐受弱酸和强碱条件，具有良好的酸碱耐受性。
实施例5：最佳感染复数的测定
按照感染复数为10:1、1:1、1:10、1:100、1:1000、1:10000的比例将噬菌体增殖液和宿主加入2216E中，并且确保培养体系的总体积相同。于37℃200rpm振荡培养8h后，常温下12000r/min离心5min，取上清液铺双平板测定其效价。结果如表1所示。
表1
Figure BDA0003197128740000081
由表1数据可知，当感染复数为1:1时，培养8h后，增殖液较为清澈，效价最高，表明本发明副溶血弧菌噬菌体RDP-VP-21010的最佳感染复数为1:1。
实施例6：噬菌体对宿主裂解曲线
将培养6h的宿主菌悬液以1:1的比例接种到100mL 2216E培养基中，37℃200rpm振荡培养2h(OD约0.45，约6.0×108cfu/mL)将菌悬液分装在4个灭菌的锥形瓶中，以最佳感染复数，向其中3个加入噬菌体增殖液0.2mL(约4.0×1011pfu/mL，经0.22μm膜过滤)，同时以不加噬菌体的作为对照，取其平均值，结果如图3所示。
由图3可知，副溶血弧菌噬菌体RDP-VP-21010加入到宿主菌BV-21002菌悬液中，作用1h后出现一定的裂解效果，约1h后，OD600值开始下降，持续作用至8.5h，9h后混合菌液开始变得浑浊，OD值逐渐升高，至12h达到平衡，但仍较宿主菌的OD值低。由于前期，噬菌体处于对宿主菌的吸附、侵染阶段，只有少部分噬菌体开始裂解宿主，因此，其裂解宿主的量远远小于宿主的增长量，因此，OD600在前1h，依旧保持一定的上升趋势，伴随着噬菌体数量的指数增加，在1h后，其裂解宿主数量大于宿主的增殖速度，所以OD600值开始逐渐降低，混合菌液达最澄清。持续作用9小时后，混合菌液出现返浊，原敏感菌被噬菌体裂解，被突变的耐受菌所取代的一种菌群交替。
实施例7：噬菌体热稳定性的测定
将噬菌体原液分装到EP管中，分别在40℃、50℃、60℃和70℃的条件下孵育30min，然后用生理盐水10倍比稀释后，铺双平板测定其效价。结果如图4所示。
由图4可知，噬菌体在40℃～60℃以内活性保持稳定，效价在1012pfu/mL以上；在70℃条件下作用30min后，效价仍可达到7.4*10^4pfu/mL，表明噬菌体具有一定的温度耐受性。
实施例8：稳定性遗传实验
将RDP-VP-21010连续传代15次，每代培养基中加入等量的RDP-VP-21010和BV-21002，每代培养时间6h，然后铺双平板，测定其效价。结果如图5所示。
由图5可知，将RDP-VP-21010连续传15代后，测定其效价，其效价稳定在1011pfu/mL以上，说明该噬菌体具有较好的遗传稳定性，适合工业化生产。
实施例9：裂解率实验
无菌条件下，分别取1mL样品和1mL宿主菌菌液(1×106CFU/mL)，37℃孵育15分钟，混匀后用生理盐水稀释至10-1-10-3个梯度，每个梯度取100μL涂布于2216E琼脂平板上，置于37℃，培养24小时，每个梯度重复两次。同时取1mL3％盐水和1mL宿主菌菌液(1×106CFU/mL)作为空白对照，重复以上步骤。选取30-300个菌落数的平板进行计数。该试验重复3次，取其平均值。噬菌体裂解率＝(1-处理组菌落数/对照组菌落数)×100％。
经计算，RDP-VP-21010的裂解率达93.1％，对宿主有较好的裂解效果，适合在养殖过程中使用。
实施例10：RTD实验
在平板上划分区域，在每一区域中心处滴加一滴宿主菌(约10μL)，晾干，将不同稀释度的一滴噬菌体液滴加到宿主菌的斑点上，晾干，37℃培养过夜。根据宿主菌生长状况，凡是不能连片生长的，称为CL浓度(CL浓度是可以完全性裂解环境中宿主菌的浓度，可以用于指导生产实践的稀释方案)，结果如图6所示。
由图6可知，RDP-VP-21010在稀释105倍后对宿主菌仍有较好的裂解效果。
实施例11：噬菌体大规模生产的方法
该工艺流程简便，可靠有效，有利于工业化生产。
具体步骤
1、种子制备
(1)宿主种子制备：无菌条件下，挑取单菌落划线于2216E琼脂培养基，37℃培养过夜，
然后挑取培养好的单菌落于5mL 2216E液体培养基中，37℃，180rpm震荡培养6h后，以1％的接种量接种于2216E液体培养基中，37℃，180rpm震荡培养6h。做好的种子液置于4℃冰箱保存备用。
(2)噬菌体种子制备：将保存的噬菌体种子适当稀释后，取100μL与100μL宿主菌置于上层培养基中，倒双层平板，37℃，培养4-6h，然后用无菌涂布棒将其刮取，将其在10000rpm，5min离心，然后过0.22μm的滤膜除菌，将收集到的过滤液置于4℃冰箱中保存备用。
噬菌体和宿主分别以1％和5％的比例接种于2216E液体培养基中，37℃，180rpm，培养6-8h，至增殖液澄清出现大量絮状物为止。
2、噬菌体增殖：在种子罐中接种2％的宿主菌，培养2-3h后，OD600在0.6-0.8，再以1％的接种量接种噬菌体种子，培养6-8h，至溶氧反升，pH趋于稳定为止。
在种子罐中接种2％的宿主菌，培养4-5h，待溶氧开始缓慢下降为止。
将准备好的宿主菌以5％的接种量，接种到大发酵罐中，培养2-3h，待溶氧开始缓慢下降，然后再以5％的接种量接入噬菌体种子，将通气量提高至1vvm，转速提高至150rpm，培养8-10h，待溶氧反升，pH升高，结束增殖。
注：发酵罐参数：种子罐200-300rpm，37℃，通气量0.6-1vvm。发酵罐：120-150pm，37℃，通气量0.6-1vvm。
培养基成分：酵母浸粉0.5％，蛋白胨1％，牛肉膏0.1％，甘油0.5％，氯化钠0.5％，磷酸二氢钾0.1％，磷酸氢二钾0.1％，氯化钙20ppm，氯化镁20ppm。
3、噬菌体后处理工艺
(1)过滤：分别采用采用800nm和500nm的陶瓷膜进行过滤，以去除细胞碎片和未裂解的宿主菌，过滤压力小于0.4mpa，减小剪切力对噬菌体的损伤。
(2)浓缩：采用中空纤维膜进行浓缩，以提高噬菌体效价。
(3)稳定剂：加入5％的甘油，海藻糖3％，聚山梨醇酯3％，磷酸二氢钠3％，磷酸钠2％，搅拌至完全溶解。
(4)除菌：采用三级过滤(玻璃纤维0.45μm，玻璃纤维0.2μm，聚醚砜0.22μm)除菌。
(5)将过滤除菌的噬菌体液体置于4℃条件下储存。
应当指出的是，具体实施方式只是本发明比较有代表性的例子，显然本发明的技术方案不限于上述实施例，还可以有很多变形。本领域的普通技术人员，以本发明所明确公开的或根据文件的书面描述毫无异议的得到的，均应认为是本专利所要保护的范围。
序列表
<110> 瑞科盟（青岛）生物工程有限公司
<120> 一株裂解性、高效价副溶血弧菌噬菌体RDP-VP-21010及其应用
<160> 1
<170> SIPOSequenceListing 1.0
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<211> 87990
<212> DNA
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tccaagaagt tattaaaggt ttacaaccac gagaagattg gaacattgat cttgattgtc 180
gtatcggtga tttaatctta actaaattgt ttaaggatga atagttatga caacacaact 240
tacttatcat gttggtatct tatctccaag agctgaggta gttgtttaca acagacagaa 300
taaagttgag tataagatcc tatcagatta tgcttctgaa agtaatattc gttctcatgt 360
aggtaactat gggtcagggc aatacgaccc ttactacaac ggcttctatg gtaatcgtaa 420
aacagctatt actcgtttag aagatgaaat taagagaact gagagttatt tagaagcact 480
taaattctct ttgaaagtta ctaagaaaaa ggtatgggag gaaataaaat gatcacacca 540
ataatcaatg gcgatggtga agtttgcatc gatgtaatag atgtgtttga taatacaagt 600
gaccaattca aacttgagct tgtagaacgt ctatcttgtg aagatgtaat catcaaacat 660
gttattgacc aagtattaaa gggctgcaca gataatgggt atagtggttc agaagtgatt 720
gacaatttac gtttaggtag tgaactacaa caagcccgag aacgtattcg ccaacaaggt 780
aacaacttac ttgttaaaga aatcaatcgt ctacgtggag tattggaaaa tcgttgtaaa 840
tatcaagata aaggttggaa tgaatatcac aagctctata atcaagttaa tcgttgttca 900
gacatttact tgtaaataaa tcttcaagca ggggttgtgc tgacacataa tctctgctat 960
atttatatcc taaacgtaag gataaaggag aaacatcatg aatcttgata tggtaaactg 1020
cattattcga tttgataaaa ctattgacga agataataat cctgtttgga atgaaactta 1080
tttgaaagag tttgtattct atgggagtgg tggtgctagt gatgcacaaa aaggtattga 1140
taaagctaaa gaattaaact ctcaacacaa aacgcaatct cgttattaca tggctgttcg 1200
tattgatata tctaaagctg tgaaacttgg attgtttgat gagggagata atctatgagt 1260
tatgatgttg ttaaaggttg tgagagctgg gattaccatg tagtggatat tttatcagga 1320
aatgaacctg tagctggttt ctttaatcaa gatatggcaa attcttattg tgaggatttg 1380
aatagacata gaagtttatc atgttgtaaa atcagtatca gtacttctgg ttggcatcac 1440
gaccatgatt gcaaaggtgt ttatgaaaaa cctgtaacct atgataagta tcgtgtttat 1500
ctttgtgggg ataatcctac agttttcaaa caaatctcac ataataagta tgaatgtaca 1560
catggtggtt ggtcattcga agaaaggtta gatagacctt ctaatgaaat ctacttacca 1620
tataaaaaga aacctttagt ggtagctgat tctatgattg agtttaatag tatacaagag 1680
gttattgaag cagggtataa gggatatttg cgatgaatta tgatccagat acaatcttta 1740
acgggaaagc tgtgttacaa gtaaatactt ctgacccttc aaacaaggtt gtattatttg 1800
taaatgataa acattcttat atcagagaac acttcttgtg ggaacaaaag tatagtaaga 1860
atggtgaact tctaccacaa gcaaaattta ttttattaaa agacattact actttacatt 1920
tgaaaaactt gtgctattct agtgttaatt ggaaagataa gaagttgaat aaagtttttg 1980
ttgatgagtg gaattggaga attgataatg aataaagaat ttaacattgg tgaaaaactt 2040
tcaccagaag atattgaagt tggtatgatt gtcgatgtct tgacggggag ttgtggttac 2100
aaggagtgta cgatactgtt attgaaacat gattttgtaa gcttcgaaac tcctgatttt 2160
gaaggagctg ttttcaacta tgagaggttt aggaataatc gatcaataaa gttccttttt 2220
agaggttaca gtcaaaactt tattgtaagc ttcacagaca atacttctta cttatctgaa 2280
caatatatcg cagaagcaat taaaaccttg tcacgggctt cacaggagct ttctacacat 2340
gttacagttg atggaaaagg tgttgtgacg gtttacagtg attatcacga agaagattta 2400
gtgtttgaca ctgtagagaa gttaatgcag tatattgagt gtgttaaaca gctggataaa 2460
tttaaataga gggtgtgaag aatgagtaag aaatctaaag tatcatattc aataggtaca 2520
ctaaattacc tatcaaacga agctctcgat gagataatca gaggtgataa ttatgataat 2580
gaaacaaaga aactagctac cagagttctg aatgcaagag gtatgtatga ctaaacaaaa 2640
ttatgatcca tcacaagtga cagtagagat taacggtcaa gttttgcagt atgttttctc 2700
tacaacagaa tcactcaaaa cacctgacat ctacagcaca caatcactac aaatcaatag 2760
tctttgtgag tttgctcgca cactaggtta tgttccaaga gatttggata tgatttcact 2820
aggttttgag attgttgata tgcgctcttg gcacacacat tacatctctt ttaagaacat 2880
gtgtaaactt cataacggat tcttacaccg tgttatccat ttatcagatg atgttaaagc 2940
ttacgacttc ttagagttaa aacatattga cattcctaca gataagcttg aattagcttg 3000
gaacaaccga cttgtagaac gtgtaaaact acaacgtgat aagaattcat acggtggtgt 3060
gaaagttcaa agtaataaag ttaagcttac ggataaaggc aaggaatatt gtgcacagtt 3120
tgatcgttac gattttcttt ttcttgaaga ttaaaggtgt gaacatggtt gttcatgatt 3180
tataccactt tcagaagtat tgtaatcttg atgattatta tggatatcgc cgtttgtgtg 3240
ttgacacaat tggttggagg ttgtatagtt tgtcttggaa gtggaaatac ttttggaatt 3300
tattagttta taaagttgcc aaacaagttt agggagatcg actatgaaag aaaaacttta 3360
tacgccagat ttagctggta gtggacaatt tgcaaggtgt gcacattgta agaaactacc 3420
aactccagaa ggacatgatg gttgtcttgg aacactacca gaaaacatta tcatgaacgc 3480
ttgttgtggt cacggagata tagaagtagc ttatattcag ttttgggata gtaagtgtgt 3540
tcgtggtaaa gaagcaatgg aaattatcaa tagtttaaag gaggactgag ggatgaacaa 3600
aaagtttcgt aaagattttc atcgtaattt tccttggtat gattacgcct ttctccttga 3660
agcaatgttg ttatggtcag agaaagcctc agaacgtcat aagaagcatg gaaacttggt 3720
aaggagcgat gatactagta agaggctaaa acttgtctca gagcttctca agcgtcttat 3780
cgctgatgat tatgatgagc cgtgcaaagt tttcaatagt aggaataaaa ctttgtcaaa 3840
aggtaataac tttggtataa attttgatga agatttcaca tacttgaaaa gtgaacattc 3900
tatttctcgt agaaatcaag ataagaaaat gttgtttatg ttattagata aacatttgtt 3960
cgagttttgg gattaaataa ggagaagtat tatgaaagaa gtaatgatga ttttaacaat 4020
ttggattgta ggtttcttga ttggattcaa tatgccaaca ctgtttggat ggtggtaata 4080
tggacaataa actcattatt aaccttctta aaacagttgt gaaagaaagt cagaaagagg 4140
aagtcttata tcgaattcct cgtgctgtcg gagggttcag tgaagtaacc aaaacggttg 4200
ttgatgggga taagttaaaa cttggtgtag aaacaattat tgcaatgttg gaaaatgttg 4260
aggagggtga aggatgatta catgtaaaga agatttgatt aatacatata ttgagaatga 4320
ccaaggtgag ttgagagatt tgtacttagc taagtgtgaa gattttaatc ttaaaacagg 4380
gtcagataga cttctccaag acggtatctt tgaccactac gattctgagt gttttgaggt 4440
gattgacgat ggtaaggttt atttcccaaa ctttaaaaac ttatcacaga attcaagaaa 4500
actaaactta ttagacctaa aaccacgaac taaaactgcg tatgaagaag tatcgattgg 4560
tgatgtggat gaatcaatgc ttgatatcgt tttcatcgat ccgaaaggtg atgtgtcaat 4620
tagaaacgct agtgtctcag agtttatttg ctgccaactt taccgtaagg tagaaaaaga 4680
gattgattgg cgagatgaaa tcagtgatta ctttgttgag tatgaaacag cagatggtag 4740
tggtgagtat agaattgata gtgacgactt cctagatgta tgtcgaaaag tattacgtgc 4800
aacaggagaa ttaggatgaa cttaatattc gagctagatg attggagtgt tgaccatgaa 4860
atcattatta aatttaaaaa taacaagttt gattggattg atcctattgt gacagtgtat 4920
gaagatgatg ggaagctggt tgtagagaat gagagtggta aattttgaaa atggaagaaa 4980
aataatttaa atagattgga atataagagg tattagatgt atgcaagtaa aattaagagt 5040
ttgacagaaa aagaagttga aaatctagag cattataagg tgaattctaa atttattggt 5100
tttgattatg aaaatgcaac agttataaaa acctttggct ccagaaacag atggaatatg 5160
gtatttaaat gttgttgtgg agaatatttt tatgtcgata acggaaagtt gggttatgca 5220
ttatcagcag gtaatttagc taaagtaaat tgtggttgta ttgatgaaaa cgcagagaaa 5280
attaaattag aggaagctaa atctatcttc tataataaac aattacggtc agaaactaat 5340
caacactggt gcacctattg cgaatcttat cgtccagaag ttaaacagaa gagtaagaac 5400
acctttcatt gcagtaattg tgatttctta gcaagtaaag ctatagtttt aggagaaaca 5460
tttgaggaaa ttatggcaag atgggattcg acaacacaca aaccttgtac caagaagtta 5520
ccttatctag aaggaaatag taagaaaggg tacaaagtaa gaggtttcac ttatatatct 5580
tctgactggt atgatgaggt aagtaagtgg ttatggatta aagttaaaga ttacataaaa 5640
acatctttct ctaaggataa cttaagtaga ataggtaaat tacacttata taaaacttca 5700
aaagatagga agtatagagg tatcttttta catagattag ttcttggatt aggtaatgat 5760
gtaagtatgg taggtgacca cttaagtggt tataaattgg ataataggaa tagcaattta 5820
agatatataa ccgcatacca gaacagttta aactcaaaga agagttcaaa aagggcaggg 5880
aatagtaggt ataaaggggt aagtttctac aaagatagat ttgacaaagg ttgtcgttgt 5940
tggaaagcta ctttgtccat ctcaggtaaa tataggacta agtactttta tagagaagta 6000
gaagcagcta agtcttatga tagtatgcta agggaatatt tacctgacga aataaatagg 6060
tataatttcc caagggatgg tgaattatct gctatctaat tgtgtaatca accctcttat 6120
tttctagagg gttttaccct agtaaatttt aactcgacat aaaaatcatc tgaagtaaac 6180
aggaaacatg atcaactcga tagataaatt atttaggtgg aatgtctttt gttttcaata 6240
agttacaaca cgtcgatagc caaagacgat tatcgttttc tagaatcagg gatcggttaa 6300
atagattatt taagcaaatc taaatctcag caccaactct gctggaatga attattagat 6360
tttcgaatca ggatgaaatt tttatagagg agtggatttt gatttgatac aatcaggaaa 6420
taatacaaac ctttcttgaa taataatcat tcaactttgt aaataagaat cattatcaac 6480
ataaattaca agtagctccg gtgtttgtct ccaccgttta acagtgcctt atctgatata 6540
aatcatatca catagaagtt ataaagcaat aatcttccct tgataatgtg atataagtca 6600
caaataataa ccttatagaa aattaaatgt ttacatgctt ttataaatcc tttatattgc 6660
acacaacatc acatactaac tactaaggtt ttattatgac ttatcgtatt gcagcctgtt 6720
gtcaactcgg tgaacttgtc actaaccgta aaggtgaaac agtatttcaa caatattcag 6780
attgtaaaat gggaactatt accgttaaaa gtatgcaatc tttatcacca acacaacaac 6840
gtgcaaagtt gttaggtaaa gtaaagcaaa acttgcaagc cttgcaatcg caaatcaaac 6900
gtgtagctaa ctttcctgat acgcaaaaga tgtttcgcat cagcagtgac ttactacctt 6960
tatatacaca tcaagatttc acacatcttt atgatgatca aatgatgcaa gttatcagta 7020
aaagcttatc aatcactggt aagattatca aagatcatga tatccgtgta agctctcatc 7080
cttcacaatt tacaacttta tgtagtgaca aagaacatgt gattgataac tccattcgtg 7140
acttagaaca ccatgtaatg atttttaatc ttatgggttt atcaccggat gatggtattg 7200
tcatcaatat tcatgctaat ggttcatcgt ttgagcttcc agaacgcgca aaacatatat 7260
ttgactatat ctcacttgaa aacgatgaga aaaaggcggg acatgacaaa gtattaatgt 7320
tgtgtgagaa gtacggtatt cgttacgtgt ttgaccacca tcactattat tgtgagacag 7380
gtgattattt agactttaac ggggatgtgt ggcaacgtat aaaagctact tggaaaggtc 7440
agcgacctat catgcatttg tcacaatcac gaggtgatac aaactttcgt gaacaatgcg 7500
cacattctga tatgatcaca gatcaagaat tgattgagta tatcacacct tatctttatg 7560
aggcagacct agacgtagaa gcaaaatgga aaaatgcagc tagtaaccaa cttgatttag 7620
acgttcaaaa gtgtgaagcc gatcacgcat aaagtttaaa ataaaccttt acaagctgcc 7680
aaggatcggt aataattacc acatcaagac aaacacaatg ttaacaaaaa ggtaggacgt 7740
tatgaacaaa caacaattgc aatcaaaact aaatgagtta ctatcgagag gttattctct 7800
taatgatgtg gtagtagaaa tacttttcga acgtcttaaa aactttaaat aggtgtaggt 7860
gatatcatga cagcatcaat gattacagct caagttattc tcgcatgtat tgtgttttac 7920
atggaatata agcaaggtaa ataatgtgat aaatgtcacg tattacgaaa gggttttatt 7980
gacaagtgaa tagaatcctt tacaatgtga aacatagaca acaaacaaag gtaaattgct 8040
atgagtacaa cttacaagat cttaactaaa ggtttaagtt taacaaggtt tgcaggtggt 8100
aactttgaat gttatctaaa tatccataag tatgtgagtt atcacgaata ttattcttta 8160
aaacttaatt accgtcaagt tgaacactta aaagagagta tgataaactt tctacgtgta 8220
agtgattcag atatatacga actttgttca ggtttcaata tcacacgcag acaggttaaa 8280
accttgataa ctgatttaca tatctacttg aacgacccaa tacataaaag ttaaaataaa 8340
ttcaaaataa cccttgacac aaacaacaaa caagtttaat attaaacacg tagaagagag 8400
aagcatctct caacagtaaa caagataaca gtcaaacaag ctagaaggtg tatcctatga 8460
caaagatcga agcggttaaa ctttacaaac aagaaaatac agactttatt aaagactgtg 8520
aacaacgtaa agacagtgtt atgttgcgtg aagattggaa caactggaca gatggccttt 8580
gtaaaagcgg tgttattact gagaatcagt ataataagtg gtccaatcct ttttaacgtg 8640
atacaaatca caaattgatt ataacaggga atttttgttt gacttgatga aagttcccga 8700
ttatatttaa ttacatagac aaggcaataa agccaaacat actgaaaaag gtaaatacta 8760
tgtcaaacca aaaacaaatc aaactagaac ttttcaaaat tgttaacgat tcaatcgata 8820
ctgaatgtta cacagatcaa aaatcctcaa cagtagcgga aaagtgccaa tacgttttaa 8880
aatgttttga aaaggagtac tatgatcgcc gtgaatcaaa ctacacaaag cggctagcgg 8940
gttggttaca aggtttacca tcaattatga caatcccgtt ttataatggc gagattatcg 9000
agatcggtta taagctagat cttatcaaga agggcaaacg tcaaaccaca acagaaaaca 9060
atgaacaaaa gtttatagat cattggttcg aattaatggc tcacaagttg atccagatgt 9120
cacaaatgaa agattatgct gaaaagcaat ttaatgatta cattgttaag gagctttaat 9180
tatgggatta ttttcatcaa acaaaacaga acaaattgat caggaagtag ttgataagtg 9240
tttaattggt cgatacaaag acacagtgag aagtcatcta tattgtattg aagagttaaa 9300
acgcgatgat ctttctagca ttgaacgtat gacgctggaa gtgaataaac aaaaaactgt 9360
cattaaagca atcgagattc ttgatcaatt agaacataaa gatgtaatac cagtggaaga 9420
tgtgtttctt aaacctgaaa aatgtcatac attagaaatc acaaaatacc aaatccttaa 9480
ataacacaca aaaggaaata caacatgttt caaacaatga atgtaattaa aacacaacgt 9540
gtaagcttca aaccttcaga gattgatcaa gaatgggaaa ctaagcaaca gcgaaaagat 9600
aaacgaaaca agcctaatcg acgaaaaggt cgtgataata aacgtaatca attctaaaat 9660
gtgatcatta tcaaaagctg ccttaattgg tggcttttct tttatttaaa cgttgaaaat 9720
ttttccttgt ttgttatact cacatcatag aaacaaacaa tacagctttt gaggctgaac 9780
aatgacttta tacgaagaat ataatccaag ttttaaagtt tttgttgtat gtcatacaga 9840
tttcggttat attacccata gttatataac agctatcaac ttggaatatg caacagaaga 9900
agctattcaa aagtttgtta atattaaaag tataatgcag ttgaataaat aaggagagtt 9960
tattatgaga ttaaataaag cacaacaaaa gcgaattaaa cgagcggtaa ggctttcaga 10020
gtgggcttgc agacaaagaa aagcaaacgt atcagataat agtaaaacgg ctcatagtgg 10080
tatacgtgca gttatttgga gagggtagat acaaacaagt tgtattagta gctaggcgtt 10140
actcgctgcc attcatagcg gagttattca ttgataaaac gtgaccaaca tcacaacaaa 10200
acatttgaca gataacagcc agtaagcaat aattaacacg taaacacaaa caatcattta 10260
aaaggtaata gatcatgaat acttcagaac taattaaaaa gtttaatagg ggtaacacgt 10320
tgaccaataa cgaactgcta gatctctacg gtgaattatg tcagattgaa gatgtgttga 10380
ttggacaggg tgacatgttc tacccgacaa ggctatttac atcaaatatt aaccaaacag 10440
ttagaagagt gtgtaaagaa agaggtttga tttaatctat aaggtagatc acattttcta 10500
taagtcgtta tatacaaaca ttagcggctt atgtaagatg aatctacagt agatattaac 10560
caataaaggg taagttatta tgaaaacata caatgaagat attcaccttg tctatacaac 10620
acattctttg gttgttacct tcctttttat tgcaaaaaca cagaatattg aattatttat 10680
ttatgataaa acacatgaag agtggaagga attatttact cacactgaag aatggaaacc 10740
ttggcactat gaaaccctgt ttagtcaaaa acgatctatg ttaggtttag tgagttctat 10800
aattagaaaa ggttcatact atgaaaacac aaattaaact tgtatttgaa cacgaaacac 10860
atgtaaaatt aattgagttt gtaacacatt tagaatcatt atcagacgtt tgtacatttg 10920
tattagctgg agaagcttta ttacttgaat atgatgtgta tctgtatatt actattcgtg 10980
ggcagattgt taccaacaca ataaccctat tattgcaatt gatcgagatt gtcagcatga 11040
agtgttaaca gattgggatg ttgagcaatt ggaaatgaaa agatttgtta tgtgacatcg 11100
atcacagaca ataatcaata actcaattac tatacaaaca catttataag ataagagatt 11160
aaagttatga gtaaacaaaa taaaaacaac acatttcatg caatattatt cattgtgtta 11220
gtggggttct ttttatgggt aactaatcca agtgatgcga taaaggattg tgagaaagtg 11280
aatacacaag aattttgtaa tgttttgaat gctgagtgaa gatattgaat aaacaaagga 11340
gaataaaccg tgaatgagaa attaagtgat ttatcactag agttaggaga ttatttagaa 11400
aacaagcttt tacctgttta tctagaaact ttatgggaag agattgaata cttaacttta 11460
gatgagccag agattaaagg ttctatagta aacttaacat tagcaagttt ttcttgcagt 11520
attaaaatta tgctagaaaa gagagaagac ggttattata attcaatgga tgatttacat 11580
ctaatgtttt atttaataac gccttgtagt gataaatttt ctttctgtca tccattacag 11640
atcaatatgg ttttacaaaa agagttgtta atgaaggtgt acgaagtaaa taaggaagct 11700
tttgacaatt taccttttaa agtggttcta tgggattaag tataaagaga taaacccgcc 11760
atagttggcg ggtttttgtt tatgtgttag ttaccattta ggataagaga ttgcacatga 11820
ttgatagtta taataactaa actctttgtt taatcttttc cactcttcaa aacctctttg 11880
ataataacta tcattcaata actctttatc ataagggttt aagtttaacg ccttgcagta 11940
attaaaccac tctttatcat ttcttgaata cattctgtta tccttggatc atactctaac 12000
ttaactgtat atatgtttag tggggtttta ggtttgaaag tatctttgtt tgttggttta 12060
taatcgcaat tcgaaacact gttaatagtg tcaaaccatt cataatcata cttatgtagt 12120
gtgttaccat ttaattcatc tagtctatag taaagagaat tattctcatc ttcattagtt 12180
gaatagatac atttagactt cttacggtaa caacgatcaa agcttatata gtaatttgct 12240
ttgttgtcta tctcatgcca attgaaaggt ttcatttctc cagtatcagg acatataaag 12300
caattcaact ctcggcataa cctagaccat tcataaacct ttttgatatg ttctaacgtc 12360
cataacgcac caataaacca acgatcagag tttgtataac tgataaatag aaacttaggg 12420
gataacataa caactggttt tacattcaat gatccgtcac tgttatactg tttgttaata 12480
acctttttca ctaagttgtg tttgtttagg gttctaaaca gcctgtccac ctttgaaggg 12540
ctaacgctta ccttatcagc aagcttccta ttacttagtg agaatgtatt atctcgccta 12600
tcaaaacact tcttattgtt aatcaactgc attaacaatt gattctcttt gttagttagt 12660
atcaatcaaa accccctcaa atcttattga taattgttct catataatca ttataacaca 12720
ggtgtgtaat tacttgaaat gacgcagtgt gaaaagcttg ccgtcccata tacaacaagg 12780
gtttgagaat gattcttgtt tacaaattcc ttatactaat ctatctaggg attaaatata 12840
taatactctt tatcaaaggt tggatctgtc ataaataata taacctcttt aatgcggagc 12900
attgggacaa cacacaatgt aaagcggtag ctttgaatag tcatacattg acaaacggta 12960
atcagtggta agaaaagata gttattgtgt aagtatatct ttaattaaaa cgaagttttg 13020
agaagtttgt tagataaagc gagagcttta agggagaatg tttataacgg agttatgaag 13080
gattattgtt gtggaatggc aaggttagat tgggggaggc ttataagatt tcgatagaaa 13140
ttgttaagca aaccgctatc gctcttacat cacaaccaca tcaaagctac gctttaaagt 13200
atcaactacc ttttaattgc acagcaattt cacataacca cctttgaatg agaatcattc 13260
taataataat tcacattctc aacaactgat taatattcga tgcgaagcat caaatatgca 13320
gtactaaatg ataatcatta tcatgtatca gcagcaacaa atgtaacttg taagagactt 13380
gagaatagtt ctcaactgta gatgtgacaa gggatgcgga gcatcgaagg atagtgataa 13440
tgtggcatga tattttggat tcattagcga ttttaggttg acagccataa ttcacgttaa 13500
gtagttctct agcggaattt tagatatttc ccatatcaac aggtataccc ttacactaat 13560
aactatacct cccaaaaatt tttattattc ttctcacaga tatttctacc tccaaaatcg 13620
actagtttta cttcaaatta atagctgttt aataaaaatt tattcactct ttgttgtaac 13680
acaacaaggt atacataact taatcttcca gattaacaga acacagtgtt ggtaagggtt 13740
agatatttgt atgacagata tttcccagag gtggtatgta gcaatgggta tgtcaaatat 13800
tttaggtagg aaatttaata agaagaggta gtaattgagg taagttactg gtattttata 13860
gtaggtgtga tctaggaggg ataatgttac ttgtaacata gatttgattt tataagaatg 13920
atctttagaa tgttacacgt aacatataaa atgataagtt tacttggtag tggagggatg 13980
aatgctataa atcttattaa atacgtttta atagggggta ttcgagctgg gaagctctat 14040
gagaggtgtt agggagatag aggtatagtt ttgcctagat ataagaaaag ccccttagag 14100
gggcttatat aaaggataat ttataaaggt attatagtga ttgttttaca cgttcacgga 14160
tttctgaaag agaggtttct cgtagcagtt ttccatcttt gaatacagtt tctaacaacc 14220
cttgtttttc ttgttctggt gtttgctgat catacactac atacttaccg ttttcttttt 14280
caatacgtag taaccctttt gcagatttct tcttactgtc cgttttaggg tctttgaata 14340
catctacacc tttgccagcc ttttcaaccc aagttgcttt tacagcagaa ccatgtgtat 14400
cacgagttac gtattgatat gagtaactac caacaccaag aacgaccata gatgcatagc 14460
ctttatcttc tagacgttta ataatctctt tctgacgttc tagtgtaata ctgtcaccgt 14520
agatagctcc gatattatcg tgtagaagtt tcaatcctgt cggggataca gttgttccaa 14580
agatatcacc gagacatacg ataagacctt tcacctccca ctcagacact ttttcagcct 14640
cataaatacc atcactatca aacatactgt catcataagg ttttttgtac caagattttg 14700
tcttatcact gtattcaact tcatcctcag tatagccagt aagcactctt acaggatcgc 14760
cactatctgg acgaacaacc agtttaccat cacgtttaag aatatcgtct ttcagcatag 14820
gtaacccttc actcacaaac ttccagaaat caaaactatc tgatacatgt gataagatac 14880
ctgttggaac cttatccatt aagtaacgaa catactctac ttctgcttct agttttgtaa 14940
tacctttctc ttctgccata gaagtgatgt aacttgttgc tgtagagtgc tccgttgctg 15000
gcactgatgc accaactaat tcattctcta catttgcatt ataatatttt tcagcaaaca 15060
agatagctgg gatcgtatct gttcctgcaa aactacatag gtgaccaaat ccactcatag 15120
ctgcagcatg tcgcccgccc ataccacgca ttgagaagtc gtgacacatg aatggaacta 15180
aatcgtgtgg caaacctgct cggtcaaaag caccaattgt gttgcgtagg tattcacgag 15240
ctgttgtagc tgatgtttgg attaaccaat tctctgttga gaaagaggtt tcaatataat 15300
tgctcagcca ctgaaaacca tctactgtat tcacgaaggt tactggtgga acaccataag 15360
ggacactaac accttctggt agagctttaa tctctagagg taaataacct aagtcatgca 15420
actcctcgaa gtgtgaagca tcataatcaa aaccgagaca actatcagta atacgtttaa 15480
tcgaacgaat agctgcatgt tttggtagat taaagaatgt ttcattccac tctttaataa 15540
gaacatcttt gatgaagtat tgtaaaccta caaatacaac ttccttgtta tctttgacat 15600
tacttagccg tccattacgg gatgtgtagt tagaatacac cttagtgata tctgggtggt 15660
aagctgattt gtggaacatt ttgtaaaaat ctgtttgtgc ccaacttgca ttaattgtca 15720
tatatttctc ctatttaaaa tttgaaagtg attttaaagc cttttcgtaa gaagtgtgaa 15780
ttgtttttgt agtggagtac caagattcat aatagccatt atctagtttc ttatattcca 15840
caactttata tagtaaacca aagaacaatg gttccacttc aatctttcgg tcagttattg 15900
tcattaaacc tccaaacatc ttgttcatta atatagttac cgattatatt agaacaccag 15960
ataaaatcaa catctttttc taaaacttct aaacctttcg aaaagatacc gtgtgttaca 16020
aacaaaccta ctttatcagc acctttctct tttagtttct gagctaagaa tttgaaactg 16080
gcagaaccat cgcagatatc atctagtata agaatattac cttctacctt atcgactgtc 16140
aagtcacatt ttacaatatt acctgtttta acatcacgaa tcttaacagc ttggatataa 16200
tcctcatgat ctaacatcat tacactgtca aaaatctttt tagttgcgcc taaatctggt 16260
gcgcacagtt tgaagtttgg tagtttacgt gaaatctctg tcaccttgtt acgtaatagt 16320
tctgtttgtg taattacttc tacattatca ataagagctg atgtaacacc gctgtgtggg 16380
tcataaatgt aaactttact gaaaccaaaa ctattcaaga tacctgcaaa taccttagat 16440
ggtaaaggtt gtcctttttg aaatacacgg tctgctcgtg cttgcgggaa ataaccaagt 16500
gttagtttaa catctttaat acctagccga tccaaagcat ccttcaccaa cccaagacga 16560
atcaaatcac gagtaccatc ttcaatctgg atttggatat gattaagacc atcaaactct 16620
aaggggatag tccatttatc tttcgttcca tgtttgattg tacaggtttc agtaccatca 16680
ctgaaagtaa cccactcaat tgttgcacaa ccttttacaa atgtaatatt actcatactt 16740
tctccttctc aatcatcaca gccttaacat taacatgacg actatcaatt tcctctacaa 16800
ccacattgta atctacacca actttgtaaa ttagtttatc accatatgtt tgttcaagtt 16860
ctttcagtcc gaccaccagt cgttgcaaca tctcatcact attgatttct tttcccatga 16920
acggcattac aatgtgttta taattgatac aagtaacaaa atcaatataa ttgttgataa 16980
catcttcttg tgttgtgtaa ttaatcataa gttgtttctt ccttgttgtt ttatattctc 17040
taaactagcc attattctat cttggaattg tctctccctt tcttctttac cataagtaac 17100
atacatattg gtgtaggttt ctagaatacc aagaagaata gggacacgtt caactgtttc 17160
ttcacacgat agttttgtgt ttatcttttg ttccgagaag gttagttcta tatctatcac 17220
ttcattaaat atacgactac tgtcaggtgt gatctctacc gtgtaaccct ctcctgataa 17280
taaactttcg tgttttattg ttagtttttc aaaataacgt atcatacata ctccttcctt 17340
caaaacctct tattccattc atccttcgtc atcaccactt cataagtgac atctgtcccg 17400
ttccattcat aaactaaact atctacgaac ttagccttat tatgacctat cagtgcagac 17460
ggtgttacta agtggaatcc tttatctaga tacccatact cccacaagag atagtttata 17520
tattcacaat ctatccatac atccctgcaa ggtttaggtg gcttttctaa agcaagatta 17580
cctgtcgctt taagtttaac ccttttccag tccataacac aacctcttaa aagaaacttt 17640
gtcttgctaa acttaactgt ttgaaggctt ccgtaagaga gtgtgtatga cgatcaacac 17700
aaacaacatt attcattctc tcataataat cttcatcacg aatctctgtc ttagatgtaa 17760
tcacaccttt atccacacca cagaagaatg atgtttcacc atgtttagct cgataatcta 17820
gatggacaac atctgtaagc tgtaatgtaa acttgattgt gtgatgttct gtcattgtac 17880
ctacagtaat ctgtttatca tactcaccag ttacactctt accatcatca gctaagatga 17940
agtgtttcac tcgcggaatt gtgtaaactt taccattatt gtatgtgata gtgagttggt 18000
ttgttgttgc aagtttctgt ttagtcatta agttttactc ctttaagttt attgtctatt 18060
cttgtcttta attcaagaac ttcattgtaa agactttgta cataatggta ttgtgtttgg 18120
tattcttcac actgcttaaa atcctcatcc ttttctacta atgcatacac tgtgtattta 18180
gtagtcaggt tttcataatc atcgtaaggg tcagtattta catcaacttg tgtcttttgt 18240
aaatctacaa acgtccaacc accattactc tctttgtcat tgatatagtt gttaatctca 18300
ttccacatct tagcctgata gtctggttga tcaccgtaga agtaatcctt aatccaactt 18360
ttacatggaa taggtgctgt agctttatgt tctatatctt ctaactttct gcgctctgca 18420
ttataacggt ctacactttc cttaaactta gtatagtcat attcgaacca gtggtcatct 18480
aattttatct cccaaggaaa ttctttcata tcttctctcc ttaattatct aaatacacaa 18540
taacattatc tggtaaacac tctagcattg gtaacacatc ttctacactt ttaccaccat 18600
gacttactgc aggacagggt agatgaaatg tccaagtagg acgttcttca gcgatacgtt 18660
tgagtttatc aatactatct ttcacaagat aagttggtga tggttttctc caatcaatct 18720
tagtttggaa tgcaccgatc cattgttctt tccattttac aaaagcgaca ttgaactctt 18780
gccctgatac taccttatca ccaaacatct tgtcaacacc aatatatgtg tcacgagcag 18840
attttgcaca tccagcaccc atgactagct taccatcttg tttgacaaca gagttggctg 18900
taaacatata aatgttatta ccaacaacac ctcttgggta gtataaacgt tctcttttga 18960
gaatcattat tcctcctctt gttgtatata acctaaagct ctcaagacat cttcacatac 19020
ttgaacaaca taagcttggt ctgtaatgtt aatggtttcc cactgaaggt atatcatgct 19080
ttgacaattg tggtcaagtg atacatcaac agacggaagg tgtttcttca tttcgtggta 19140
aatgtttttg tgtttacata caatcatatt tcctccaatt caacatcaac aacaaactct 19200
cgattaccaa gagacttaat acttacctta acattgtaaa cactatcata attgtcacca 19260
agatgctttg caagaatctt acgaacatcc gtgttaacat tatcacgagt taagtgacaa 19320
ctatcgtata acatttctag tgtgtttaca atgtcggatt tgattgtttc tgtgttatct 19380
tgcataaaaa taaatctcca cacctaatta agatatggag attatgaact aggttttact 19440
tgttgtcaat gtttttattt aatccttatt catcttatct acaaacttaa gaatattaga 19500
aatacgttca atttcttcac cagtaaactt atattctcca atcttaacaa gtgtcacatc 19560
gtgttcttcg cttgtataaa actcagcagt aatatcttca tgattaacac catctacacc 19620
aaacgattct agtgtttccg taattttcat tttacacctc caataatatc tacgttttta 19680
taaaatgtag ttttaccaac accattaact tgtttcataa ggacaatagt ttttacagtt 19740
ggggttgttt caataaagta atcaatacaa ttttcattgt aactacgggt ttctcggtat 19800
aaggtgaaac ctttacgagt gatttcagct tgccaataaa ggaattcttt ttctgtgatt 19860
gcaactgtgt tcataagatt aatccttttt cagtaaatca ttagctgact ctacgacagt 19920
gttataacgt tcacgaagat gcttatcatc tttcacatca atccaatact gcgttaaaag 19980
ataattgata catttacata aacgttgttt atctgttgtt ggattaacca aggcatcact 20040
gcctgcgata agttttatac tgtctttgat atcgttcatc atacctcatt attcttatac 20100
ttattaacaa aggcagcttg tgaagggttt aggtaacaat ccttcccgcc attgaacaag 20160
tttttaattt ctaaatgttc tttattatat aaccaaacac caagatagtt aaatttattg 20220
tatgcgttta aatcatccaa tcttgtcttt tcttcactgg ttaattctat ccacccaaat 20280
ctataaagct gttcttcgta agtcataaac atattagagg ttttgtcttc taaagttctg 20340
ttaccaacaa ctctatccca taaagacggt ttaaatctac tctcagcata agcaatttta 20400
tccctaagca agttgtctgc ccactcaact tctttttcgt agcaatctaa tgctctttta 20460
atttcctctt ttgtatataa agcagcacca gtgaaaatac tcatctatac ctccaaatcc 20520
gcttgaatac aaaatgtgct ttcatcaaag tgttcatctt cccactcaaa atgtgccgat 20580
tcgattaagt aatccatcat aatatttgta atgtgttgtt cttgcatatt aatacctctt 20640
ttatttaata cgttgtttat ctagaatctg ttcgtatgtg tcagcttcta ctttatcata 20700
tcgtggacgt ttgtaaatag gtaagaacaa actttcttga ttagttttct tatctttcgt 20760
gatgctatcg tactcaactt caataagggg tggttgctct tttacgtaaa gtttacgttc 20820
ttcttcactg aaaccacttc caacatcaac tttaattgtt ccacactcag attcacaatg 20880
gaagccacct aacatccctt catacttgct cccaacttct gcataataaa aaccaattac 20940
cttaagatct gctggctcct tacgttttac ctttatattg aaagcaggtt taccaacatc 21000
tttccatgta gcatccatgt ccttggcaat cgcaccttcg taaccatcac gaacatattg 21060
ctcgaagact tcaaatactt cttcaacaga tacgttttct tttcgttcga tgagatgaat 21120
tttaggcgga acatagttaa tagagcacca gtagtggtat tcattcatca taagctctaa 21180
acactctcgt cgcttcttat tactttctgg gtactcacct tttggttggt aatatttagt 21240
atcaatacag tcccatactt ggagtatagc attctctttt tcttcttcag aagctgttcc 21300
ttttacgatt tttgtaataa tgccgttacc actctcacga gtagctttat caagagagta 21360
tactaactca ccttcaagag caaaaccatt gaatgcacca caagataaat gctcttgtaa 21420
acattcaata tcaagagggc taccattacg tgacatcatt gtagattgtt caccacaaat 21480
acttgctgca taactaccgt cggattttag ctcaacagct aggtttttaa tagaatgcag 21540
tttcttcaat gacttttcat tcatagaagc agcacccata cgaggtggtt taacaattaa 21600
acctttccac actttgttaa tggttgcatc tttcacacca acttttaaat cttgtaatac 21660
tactttctca agaacttctc ggtcacactg ttttgtggta aataataatt ggtcaagata 21720
ttgtcgggct tcgttacctg tgtaaatgcg gtcagataaa ttcttaagta aagggagtgc 21780
ttgttccaaa gaacccagtg ctgttggtaa tgattccccc ttttgtaaat tcttaatata 21840
gaacttaata gttggtgagt aagctaaacg gaaacactct ttcaaggttt cattatcttt 21900
atgttcaagt aagatagctt ctttctcatt cttactgctt gttgcagcca cttcttctaa 21960
aatttggtaa acttctttcg tcattacttt atttctcctt cttagggtaa aactctttag 22020
ggatttgttg attactactc caatcaaaac tttgaccacc cacacactta acttttataa 22080
ctggtgtttt accgaacata ttactataag tgtatacacc accattatca ctgcatacat 22140
aatccatctg tgctttctgt aaactattag gttcacaccc tgttaaaagt aataaacctg 22200
aaacgattaa aactttcttc atactcatcc ttctcctaaa tttctcaatt gatactcatc 22260
tgatatttca tcaacatgga ttagttttcc tacaatctcc tctggtgaac cgaacccttt 22320
atcacctttg ttatcctgac agtggtattc tgtgtgatga tatttataac ttccttcaac 22380
gaagaaccat ttctcgttca cccagtaatc atctaaatga taaaactctg tagtcatatt 22440
gtcaatcaaa tatttaatct tttcgtcaga tagttgtttt tacgttcata aattatttat 22500
ccaaataggt tgaatttaat gcagtaaaga ctcccaccac taaagttatg gcacaaatgt 22560
ggaatactcc ttgcggtaca atattacctt gatagctagc tacaatcatt gttacaaatg 22620
ttactactga tataaacaaa gaaattaaac ctacaacacc gtttgtatat actaagaatg 22680
ttttcataat tatacttcct ctaataaaca aaaatcctca tcccgaagga caaggataat 22740
ataaggttat ttgttcaagc tgtcaacgat ttctttcact ttctcatcaa gtttttgtgc 22800
aagagtatct tgacctatat tgtgtgggaa tttcacacca agtaaatctg cgtaacgttt 22860
tagatcaggt aatccgaatt catcctgtgt ctctacgaaa gatgtgaatg gtggttcacc 22920
ttccatgtgg atatccatca aacctctctt atcaattgtt aggataaact ctccctcttg 22980
aaaagtctct ccacaaactt caaaaacatt atcatctagg acacaatctt cttcagatac 23040
tacctcacct tcaccaaaca gcatagctaa gatttcttct ttagatttat cttcaaaatc 23100
ttctttggag tagagcggtt catcttcttc aaactgtttc atgaattcag attcttcctc 23160
ttggaaaagt tccatacgtt tctcaagacg ttccatgatt tcgtcagcat ccatccaaag 23220
gtctttgcca tttaacactt ctgaaatctc atcttcagac atgaagtgac ggtagtactt 23280
tttgataaga cggtctgttg cttttgcttg atgttcaaca tattgacgga cattattttc 23340
ttttgctaca tacccatatg tgcttgtgtg aatcatcatc gtagcatcat ctgtaacacg 23400
atattcatca cctgctaaga aaattaatgt agctgctgaa cacgcctctc cttcaatctc 23460
cgtaataata tgagcttgtg attgagccat actattaagg aaagcttttg ctgtatctaa 23520
acgaccacca ccagagttaa tcagtagatg aacaacatca ccttctttgg attgacggat 23580
tgttgctaac tcttcttgga atagggaagg gtcttcaata tcacggtcaa cacggattgt 23640
gtattttaca ttaataggtt cagcttgggt tcgaatgaaa ttaactggtt gctccatata 23700
ttatctcctc tttataaaaa cttatctaaa tacacttcaa tatcatcttg taagaaaaga 23760
taatcatcga tgtttctgta tactgtttca agtgctctca tttcatatga attacaccac 23820
acttgattct tactaatctt agccatcttc ataacatcaa ccacaacttg taaatacttc 23880
aactttctct catactcacg gaagatttga gattgctctg agaagtattc taaagcgtct 23940
cctttcattg tccacttaca aacaggtttg acagttttac gtttcttcca ccaagacagt 24000
ttattgtaat tagttacttc tactttccac tgctctttat aagttttatc aatacgttct 24060
acaactgtgt cataagcatc ttcaatctta cgagtgattt cttgtggtct aggaaaatca 24120
ttaacttgac ggaagaagtg atctaagtat tcgtatggaa ctttaccttg cataattatt 24180
actcctcttc cattgcagat ttcgcacaac caagtgagat taactttttg aataattctt 24240
catttgaata ttgatcaaga ttctgagatg acataccaag ttcaatacaa gaatattcta 24300
agctttcacg agagtaattt tcccaaggtg tgtatgaatt tgttccaaca ttaattttat 24360
aatttgacat aacattatct ccgttttgtg aatgtagttt aactatagat taatattgga 24420
actgtgtcaa gggtgggtta gaaattatcc tccacaaatt ctgctacacg agaacgaact 24480
acaccttcta acctcataac cttagcgaaa gggacgccgt gaaacttctc tgtagcgtaa 24540
gttaaccctg atgttcgtgg acttacgaaa tcattatcaa tctgccccaa attacccatg 24600
ataattactt tacagtactg acctgcacga gataggattg tcttagcttg agatgctgtg 24660
agattctgaa attcatccac aattaatact ttatggttaa cagaacgtcc tctcataaaa 24720
ttcaaactct taaattgaat gaatgcatcc tcattccctt cttccatatc aattgctgcg 24780
gacggttcaa atttcttatt tttactttcc ctcgcaataa tctccatatt atccgtacat 24840
ccagcaagcc atggccccat tttttgggct tctccacctt ttaagaaacc gatctcttta 24900
aattgagtat cattactccg tgagaagata atcttttcca tacgataaga ggacttttta 24960
cccttcacca tttcacaaga cgctgccatg gctaataagg tcttaccact accagcttta 25020
gcatcaagga tcacgatatc gatttcaggg ttgagcagtg catctaatgc aatagcttga 25080
tgtacatttc ttggtttaat acctgccgct ttacgagaca ttaaatgatt gtctatatta 25140
tcaaagcgga aaatacacca tttatcatcc tcagtataat ccaccagttc agcagctacc 25200
ccatcatcct cgttaattaa ccaatcattt aacgcaatag acgctgtttc atcttcctct 25260
agtaattctt gaatacatga tttagctatg tgtgtttctc cgcaagattt agaaatcact 25320
ttatctgtat ctagagatga taaccatcca tctggtacat ttaggtaacc tttagggaga 25380
aattctgggt cttctacaat aacatcacta ctaaattctt gaacttcttc aacacctata 25440
cctttcgctt tcagtctcat acaaacatca ttagatacaa gaatagtttg tgtgttactg 25500
ttctgcagag ataaggttac attaataata tggtcatcat tacatgtcgg ttttatataa 25560
gaagtatcat cagttacatc aatattctta gcaacaaaga gtttacctcc ggtaggtaag 25620
tctactccat gtttaatagg atctagatta ccaattactt catccaattg tctaatagct 25680
gctcgtgcat ctcttgcaat atctctatct gaacttttct gtttatcgag ttcttccaat 25740
acagttaaag gtacaaccac attattatct tcaaatttta acagagaatt tgggtcgctc 25800
agaaggatat tagtatccaa tacataatgt tttaccatgc gcacctcctt agtgttattt 25860
gaaatctgtt ctattaacca accaacccca tacaaacttc tcatctttac ttcgtctagt 25920
agctaagtcg atatagaatg caccctgttt aatatttaaa gcattatata aaaccttttc 25980
tcctttctta ccgcgtttat tcaaaaaaga tttcaatgct gagcatgttc ctttaccaat 26040
aataccatct tcactgatat ctttatagta agcttgattg ttgttgaaaa cgtttaacaa 26100
ctcttgtaac caacgtgacg gtcttgaggt tccagtattt actcctgtgt caaacaactc 26160
ttgtgcgata agttccgatt ctttagcgac gaggttaaat cgagggatat agaaataatc 26220
ataagcgtaa atatctttag cgaattcaat cggcatcgtt ttcatatccc cgttccagtg 26280
atataaattc caactacctt taaacttaag agcggtttta taagtaatac cccaattggt 26340
ctcaccacct gaatcactag gatcgtctac gtaaccaccc tcgtgtttag ctgttgtcat 26400
gataaagtct ttaacaaact ttggagattc tttgtaacac ccaataatac tcataattca 26460
accttctcct taaaactatc aaccattcgt aaaatctcac gaattcgccc aaacaattgt 26520
tgttcttctt cactatcatc tttaaacgta gagaagaagt aatcacctgt gtcaccaaga 26580
ttatctaagc gataacctcc atctttatga tttacaaatg tgaagtgttt accctcttca 26640
tacagaaacc aatcctttgt ccgaaacatt aaattttcca taaaatctat cctcctattg 26700
tttgatgaat attagtttat ctaacggttc acacttttct aaaacaatac cacaataacg 26760
tttcagcatg gcagaacaca ttttatcttg tggacaaacg agggtgaacg tttcatttgt 26820
tgtggtattg tgtgctgtaa gtttatcacc tttgttgtaa tactttttaa tgttagttta 26880
cctcttggaa tagatcgttg aatgagattt cttctctctt gtttacccaa ctccctacaa 26940
actcactgac gctgtcttca taagactccc agtagacatt cgaaccccaa tcaaaagttt 27000
cttcgttgta gcaagggaaa ttgttttgaa gataatcttt ttgccaatca gatagtttac 27060
ttaattctag gtaatcacct tctttcaatt tataatctga gcgtattaat tctggttctt 27120
caacttcctt cggtaacaaa tcttgtggtg ttactttgtg gaaacggtct ttatgtaaac 27180
taccttcgat acctttgtcc ataataattg tataattatc attgtttaca ttaacaactt 27240
tataatcctt accgagagtt acttgaccta aagagtcatt acaacacgtc acccaacgag 27300
gtttatcaaa gattgattct tgtaagaatt ctgtatcatc ttcaccccaa aacatccccc 27360
agtagaaatc gttgaatgtg atttcattct ccgaaggagt gcagtattca gttgttgtgc 27420
caaaacaaga tcctctggta actttaaact ttctataacg ccaaaaaggg aattcaggat 27480
ctctaaactt atctcttgat gatggtaaaa tatccagatt ctccaaacac cattgctttt 27540
gccaatctgt taactttcga acatcgaacc actggccttc tttaaattga aatttcataa 27600
ctttctcctt tatctttgtg taacatacaa cgtccatgta agtatgtatt agtttgtgtt 27660
aggtgtcaat ggtatttatt aaaaatctaa atcgtccatg tcacccatat caaaatcttc 27720
tacatcagtg ctcacagcac taaccatata gtttgttgga tcggtttcca ttggggcagt 27780
ttgatggtta ttaatattca tccaattatc ttcaaaccaa ggtagtgggt ttgtaactgt 27840
tctgcggaaa tcttgtttca aaccaaggtt gtcatacact tcttgagcat tgtaacgcac 27900
ccagtcattt aacaaaccct catttaaccc taccattcga cgtccttcac taaacaagta 27960
tttattccat gccaattctt tagtgacaac atcatctacg atttgtttaa tctcattctc 28020
gtataagtgg aataccattt tccactctcc ttgagtgagt aggacatctt gcagagcatg 28080
tctctgaact tcaaaatgaa cagtttcatc cataagaatt ttttggacta gtcgagcagc 28140
acctacaaaa tactcttgct cggctaacgc aaaagtagct gaaaaactag acatgaaact 28200
tatacgctct agtgcataaa ttgctacaac acctttaagg atgtaaggtt tgcatttctc 28260
aaaatcccac tcacctaaac tataacgtaa tccagcctct tttaactcac ctaacgcctt 28320
accaaataca tactgtctat ctaaaacctg ctcgtgctta taaaccatat cgaaaacttc 28380
ttgtgggtct ggaatacagt ttcgaacgat agatgagtaa gtttcactat gcaacatctc 28440
attctccccg atacgggcga taacgtgccc atattcccag tttgttataa atgggttgag 28500
aagttgaggg atacttgttg ctacactatc taatgaccac tgataagcta gattataaag 28560
catgatatta cgtgtattgg tatcacaacg gagtaaatcc attcgtgtct gagataaatc 28620
cacatcatca gatgcccaat ctatctgtcg taatttttcc ttgagattat agaagtgtgg 28680
gtatttcatg ttaataccgt cgtaaaggct aggatcttcg ccaccgaaaa gtgggtaatc 28740
tcctgtttta tgagctacgc ccttatcatt gaaaatactg ttcttggtca tttaaaataa 28800
ttctccttga aagagggagc ctaaactccc atttaatatt attatagtgc acatccacca 28860
gatgaacagc ctttatcttc cactacttct gcttcggtgt caactgcttt atcagatttc 28920
ttttcctctg aacctgagtt agttcggctg ttaacatagt aacgtgtttt catacccata 28980
gctgtcatat acaagaagtc tttcaacata tctttacctg taatcttctt cttagtgaag 29040
tctaggtaga aatctgcact aatagtttgg tctgtgaacg cttgaaagat tgcataggtg 29100
tcaatcaaat ctttggtttc aatatcccat gcatattcat aattttgttc tttagtgtcg 29160
tagttaggaa ccaagaaccg tgtcatttta gttgcgttag acttaaccaa cttaccactt 29220
cgagcaggta aaatactatt ggtaccgttt gtggcgatac tactgctttc attaggcatc 29280
atggcaacaa gaacactgtt gcgtagtcca ccttgtgcaa taatacgttt acgtagagac 29340
ttccaatcct tactgatcaa gtgaccaaac ttcttcttaa cattattgtt catggtatca 29400
atcggtaacc atccatcagg ccatttagtc ttgtctatgg ctgaacacaa ccctctttcc 29460
tctgctagac gtaaacttgc tttgatacaa gaatacatat gttgttcagc aaccttaaac 29520
atgtattctt tgccttcttt agatgaatat ttaagattta catctgccat atgacctgct 29580
aagtcggtaa taccaatacc aagactacga cgtgcttgag ctgttacctt caaattaggg 29640
aatgggtagt ccataatatc aataacatta tcaatcatta atgctgcgta ataggctaca 29700
tcgtcccatt cctccaaact cacacgacgt gcaacaatag cacttaaact gcataaacca 29760
atttcaccgt tccattcttc tctgtataaa tcgtctactc gattaaaacc gctcgttggt 29820
aaagcgattt cctgaccttt tcagttttga ggtcgctaca cctcttccac gatttctcat 29880
gttgtcggac tatatcatca tccgttctgg atgctctgcg cttcgaaaca ccgatagctt 29940
gtgttcctac ataatagtct ctagaccttc aattaagctt ggctccgagg ttggcatagg 30000
ataattccct tagctttccc agaattcaca gagttattcg acatatgttc ccatatgaag 30060
ctgcatcagg ttgtttacag attactcgaa tagattttat ctttaaatgg tgtgttacgg 30120
tttagttcat cagtccggtg taaatacact tgaccacttt cttgagattg aatcagaata 30180
ctcactgcaa gttttcgtgc gtctactttt ttaacattgg gattatttgc ttcatgctcg 30240
taataaagtt tctcgaactt agactggtca ccttcgtaca ttgcttcata caaatcaggg 30300
ttatcagcgt aagaaactag catccaatct tcaccatctt taaccttctg agcaaataga 30360
cggttatagc cgattgaata gtgaatacca tctacacgga ctgtaattgc agtcttttta 30420
cttttccatg tgataatctc attaatctct gggtctaaga cgttaacatg aactgtagca 30480
ctaccgcctc tcgatgattg taggttagct gcaacagaac tttcaaccat cttgtagtaa 30540
ggacgtttac cttggtgaat ggttgtcccg cctcgaacac catctccctt actccgtgtt 30600
ttgatgtgag atccgattcc agacgaatgg caggtcatca tataagcaat atggtcacca 30660
gaagctaaac tgttagctgt atcatgtgta gtataaacag cgcaactagc gaaaccacga 30720
tctggtgttc gtaatttagt cataaatggt gttggggcac aaatcttttt atcacttaaa 30780
taagtatata attgcttaac atcctgaatt cgacgatctt tgggcatacc tttcatgttt 30840
gccattgcca tacccatgta tgtaaattgt gggctctcta gtgctctatc ctctacacgg 30900
ttacggatca catacttgtc agcaatctgt ttgatttctg gataagaaga tttcaaatcc 30960
ttaccgtggt ccatgaaatt gttcaaatcc tcaaactcat cttcagagta aggtaaatct 31020
tcccagtaac cttgagacac catattatca tgatgttctt tcaaggtagg aatattacta 31080
tagccaccga aagcttcttt ataaagaaca ccagctaaaa cacggcctgc caacatgaaa 31140
tgtttttcat caaaagcctc aacacataca tcaatcagtg ccttgttaat atctgttgtt 31200
ttgcacttat catcaagacg tttataaacc tttaaggaga tatcactcca attaacttgt 31260
cgatctgctg cccaacgact tttcttattt aatttttcag catcaaaagg gacttcttta 31320
ccgttagcct tgataacagt tctaatattt gtcattacct attcttctcc tttagttata 31380
atataattaa tctcatctat ggtaatactt cttacacctg ctgtgaagct tgcctcttct 31440
tttatttggt cttgatattt ttctatgtaa gccttatcac ccttaacgta gagactacta 31500
gactttgtaa attcttcttt tgcaattaat cttcccgctc tcccaccaac tcttctcagt 31560
aggaaatcat gctctttttc cgaacctttc tccaagtatt tattataatt gatgacctct 31620
cttggagatt ttacccacac ctgaaaaaca caaggaacat cgtaatcctc tccgttgagt 31680
atgaaactat ttttaggtaa gtctttttgt aaggcaagag atgcttggtg tgtcaccttt 31740
tccataaaca acactttctt aaaggtcttt ggtaagataa aagctaccac ttctgcaccc 31800
tcttcgaaag aatggttgat aaacttcaaa gccagtgaac ttgcaaaacc aaatggaggg 31860
ttaccaacaa caactttacc tgttaaattt tcaacggtta agaaatcttt cttagtaata 31920
ttactcacct tgggttctag atcatatgct tcccatttca aatctggacg taagaagtta 31980
cctgtcccag cactaggttc tacaaaatca tagtctttag aacctaaaaa ctcgataagc 32040
atattaagac attctgtagc tatttcaggt tgcgtgtaat attgatctaa cttattcttc 32100
ttgctcacaa atccaccctt cctttatcat caactggtaa agtcttccag tcataccaat 32160
acacgagaac gttcttaaca tctttggtgt ttactaatat aactccatca tacttctgtt 32220
tagcatcata caaaatatct acaccatctt tatccctacc acaacctttg gttttagaag 32280
gggctatatc aaaggagtca tctcttgagc accagacttt ccatctacgt tcttggaaat 32340
cctctaaaca aactaaacgt ttatcagaca aatgtaaaac tgacaggtct gggaagatgt 32400
cttctaagat gtaaggtaaa actttagcaa caccactagt gtctttccac aaccttttac 32460
ttacaaaatc ctgaaagtaa tcggacaagt ctagttctat tttcataagc tattcttcac 32520
cactcatacc gttatcccac tgatcatcaa gttgtcgcat actaacagct tgataacgtg 32580
cttgacggta actatgttca tcaccaacgt ctaaaatatt tttaagttca ccaccgtgaa 32640
taaaatcacc aactagaatc tcacctgatg aatataaatc tttgtaaggg taatttggtc 32700
gaatataccc agtgtaaact ttcatctcct ttacttcgta agggcgttta ctattacgaa 32760
caaatacagt ttcactttta taatttcgat gataacgtgt atcttttgtc agcatatcat 32820
aaactgcacc ttctggtcga gcaacttcct gtaactcttt agtttgtcga gaacgacgtg 32880
ttttcttatc tgcagctttc tcatcaaact ctttctttgt ttctccataa taccagtaga 32940
aacctaagat gtataatact aaatcaatat ctacatttcc attttcatcc cgtagaggga 33000
taatttcacc catgtcatca cgaaattcca tatccgcaac tttctcaata aaatcttcac 33060
ttacacttcc gtgtagaggt gctgactgtt ttgccataac ttctcctgtt ggacaacctc 33120
atctgttatt tgaaaggttg tccattatct caaattagtg ctatcaaagc aagaaatcaa 33180
atctcaaaat aagattaaat ttagatattt gttctatgtt acaagtaaca ttttattcat 33240
cgtccggaag gttttcttca accattttca acagtgttgc ttgattatgt tcaaggaaac 33300
gagtaataac atcttcgctt gcttcgagca ttgttgttcc gagtttctct tggtaagaat 33360
aaatcttttc tacagcagaa acataatctt ccatttcatc ttctgtcata tttaacacgt 33420
aagcattagt gacaacctct gctgccagtt cttgctcttt tgtaagcttg agaaattctt 33480
gtgattgttc tggtgtaagt aagagatttg aacagatacc ttctatttgt gtgtcaatgt 33540
tgttttgcag gatgttaatt agtcgttgtt ttggtgttgt cattatttct cctttagctt 33600
agagcatcta tcgtgaaacg ataagcactt cgtcagtttt acctttacct tttcggaaac 33660
tactattgtt gtagctcatg tttaaatgat gcacttcaaa tacatcttta tgttcttcac 33720
accaatcaat caactcttga tgaactttac cacgattctc aaacacattg gagataacaa 33780
cttttgcacc acgtttttgt aattccacac aatattcaag aagtgttgta ttgtcattat 33840
cgtcccaact attctcgtta taagttgctg tggatgttcc acctccatag ggagggtcaa 33900
gatatacagt agttccagac aaattcccac tctccatcaa tcgttctatt gcatcagcga 33960
agtcttcatt ccacaactca gctttctgaa ttaagtctcg atgagtcatc aaacggtctg 34020
tatcacaacg atgtcgttta ccaaatggaa cattgaatcc tttactactg aatcgcatca 34080
tattactgtt tgaccgacat tgaagattat ataacattgc gtaatctttg caaccacctt 34140
tgttataatc ttcacgaagt tttaagtagc cgtctaagtt atgttcgtaa tcagagttaa 34200
tttgttcaac acgttcaatt tcttctggtg tgagagtttt aacccattta tgtaaaccgt 34260
gtaagaacca agctttctca ttacagatga tgttgtcgaa aagctctgtg ctattaatgg 34320
caatggtcga acttccagaa aacaaatcca gtaatacttt acgattacca tcttggagat 34380
gaggttttat tttcttaaac aattttctct tgtttcctgc aaggtaaatg gaggattgtg 34440
ctgtcattaa tatctcctct ttaaatttac acatccttgt gtaataaatt agtttaccac 34500
ttagtatgcc catctaataa ctggcgatgt tgaatccaac cttttagatt acctgaccag 34560
taattacctt cacggtctgc atgggttaca ccatcttctt cagtatctaa ccaagttgaa 34620
gggtcacaat attcctgcca ttttgtgact accataggtg aagctacatg ctcgaatggg 34680
gaggcgtgga ttttactacc accaacacct agtcgatcat aaaccttcat agctttatca 34740
aacgaattat caatagatct gtaagaaact tgtgcacaac atgaagatga gatggcaata 34800
gcatccttaa cattaacact aacaccatca accttatagg ttagttcttc agggtaacgt 34860
ttatgttcta catatggtgt atgccattca ccagctttaa gttcttcagg attactttca 34920
tcgtaagcgg acttcataca ctcagcaagt tctttgattt cgggttgagc atcttcatca 34980
catcgtaacc aaaagaagtt attatactct gttgctgtaa caacaacctt catcatttga 35040
aacggttcaa gtaaacggtt tacgatttgc ttatgtagtc cttgatgttt taagacttcg 35100
gctaacattg tgacactttg agcacaacca ttccataaat tttcacaaag tttgacatct 35160
tcatgttctg tgtcagcctg cataccagat ttattaatac cccagtggac tggttgtgct 35220
ggactcttac ggacttgctc taacatctta tcgattggaa cggcacgact actcattgca 35280
ttacgagaga ataaacggtg tgtcattaac tctgagtgaa tgaatcgagg gtattctagt 35340
tcaaaagtag tgatacgatt atcaccatta atagagtctg caacaatctt agctttgata 35400
ttaccttcac cgtgtttttc tactttatac aattcttctc tccttactta attcgacgat 35460
taatttcgtt agtaatttta ttcttctgag aaccttgtgc tcgtttcaaa gcttcacgta 35520
gttgttcaag cgacattgat ttcacattaa ctttattctt ttcttttgcc atatttgtta 35580
ttctccttct tgttccaaca tattcaacgc aatcattaaa tcttcacacg ccaacgccat 35640
attcatgaca ctacgtgagt ctcgccaaac atcgtctgac ttggtaggga tattaccttc 35700
ttcaaaatgt ttttgcaagc actttgttag gaattgtttt gtattattgt ccataatcat 35760
agcctttagc aatttggatg actttagttt ctactacagt attctgtcct acaccatcac 35820
acggttcatg gatgtttaca atctgtgcag gaatcgcatg aaaacccata atacctgaat 35880
attcaatagc atcaaaagca accccgaacg cagctccatt tacttgaaga ttaccattat 35940
catagttaca ataatggtgc atatccccca ccactaaacg atgaacgtat tcctgtaagt 36000
tattagcccg tttgtttcgt aaagtcatca aagctgattc tgaactacct ttacaattgt 36060
aaccgtgctc agcaacagtc agtttcccgt aaatacgata agttgcaaag tgaccttctg 36120
gtaggttaaa ttctatgtga tcaaaccctg caatcttaca catattctcc catgttttat 36180
ataatgtgta gtccatccca taacgtccag ctttgaacat atccatacct ttatgttcac 36240
tagcaccatg attaccgcaa acacccacaa attctacagg tatccctaat gaaaagatag 36300
gtgctaagat attaaaccat acatcttgaa tacagttagc catttgttct gcattactag 36360
tatctgttga tgctgttgac tgaacaccgt gtttaagtgc atcctcaaaa ttatctccga 36420
gagcagagaa aataatcttc tcaggttttt cttgtttgat aatatttaca agttcttgac 36480
cataataagc actagatcgt gaagcaatct cactgttcca tgattcactg actttacccc 36540
actgccaatc tgaccataga atctccactg tccttttctt tggctcagac ttgaaaaagt 36600
caatttcgtg aaaaaccaga gacttagttt ctaatttaca ggagttgatt ttatcagcaa 36660
gtaaacccat tacactctct aggtctacat ccttctcttg acggtcaaac tcttcccgct 36720
ggatcttcct gagctgcgta ttgctgcgct gggcgctacg taatctctta gccaaattac 36780
ttacgttcca atcaggatgt tcacataaac cttctaatgt ttgttcagga acaacccaat 36840
cttcttcagt attcacaaca ccattaagtg ttacaccaaa ataattacca tgtttcttat 36900
acacacgttt aatattacga actgtagatt ctttagattt cttacctaag acttctacag 36960
ctacttgtgt attaccttta ttatcttcaa acaaaccttt taggattgct aagattttct 37020
catctgataa acgtttcttt gacaaactat ttctccttac caaacatctt gcattccaat 37080
caactcagct ttaatacctg tcaattggta gaacttgtgt gctttatctt ttacatcttt 37140
aatccaagta tcaaactctg gagataaggg gtctacatca tttactgtga aacctaaaac 37200
ttgatcatca cttggtgaat cgtaatgata agaatatgtt gatagacctt gggactcata 37260
ccattcatag aattcttcgt aatagttacc ttctgtttcg taaagttcac aatcattatc 37320
ttcaaaagtt actgcagatt ccacttctga acaatttgca ccaacaatca ttccacctga 37380
aatatcaata cccatcattt ctcctcattc atcaactgtg ttttataagc ttcctttagc 37440
tgccacaatg tttctgcagc tttagttaca ctcttagctc ggaagatctt tccatacaaa 37500
ccaacatagt ttcctgtatc attgtctttg atataaatac atccatccaa cttgttacgt 37560
tccgtaatgg aaaacttagc gtttgcaata tcttcatgat tttccaaatt ctctctccta 37620
aaattcacaa tgttttaatt cacgaacaat agatgtgatt gattcagcat aactcttatt 37680
agggatatta cctccattat acatcccaac acctttgaac caatcacctt ttgcacgttt 37740
ctgccaataa acaatatctt taacaccctc ttgaatactg tattctggtg aagttatcat 37800
attctcaacc cacataccag cttcgaaagg tgacatattc tttgttttcc aacgagctac 37860
tgtgtgcaat acgccaaaac tttggtcatt aatgttacct ctgttataac gaacattgta 37920
taaaccaagt ttactttctt tccaagcaat agctactgtt gtcaaaccta agtaatactc 37980
ttctccatgt cggtaagcaa tctccatgat atctttctga taaggtgtca attgttgttt 38040
gaatccacat tctccagcat caacatcctt catatacaga gatagaataa tacaaaagat 38100
gagtaagaat aagctttcaa ctccacgagt ttctttaaat tctttgagtt tttgcttaac 38160
tcttactctt ttcttcgttg gctgtttcat gagattttac gctccacaac ctgtttaccg 38220
caatccttgc atgtttgaat gtggaaatct tcactttgtg tgtaatgcca aatctcatgc 38280
ttacaacgaa gtcgtttaat aaacatctta acttggtaaa taaaaagatt aatatcgaac 38340
ataatactat ctcctttgcc gaaggtaaac acttcacttc agtgaaacct ccttggttaa 38400
tgttgatgta atcttagcac atccctgtgt gaagtcaaca atttatttaa attattttca 38460
atctaacaaa cctaactctt catgagataa actttgcacg taatcaatct ccgttttatc 38520
cacatcaata gcttggagaa tatcataaat tgacagataa cgtcctgtat ctttgtcata 38580
caaatcttca tctggcatgg catcatacac ttcttgagca gatagaagtt tgatcgattg 38640
tagacggttg atgttagtca ttgttgttct cctgtgtaag aatttctacc acttgtttac 38700
gtcgaagttt tgcattgctt tctggtgtaa agcctttgga tgcaatccaa gatttttcat 38760
ctcctttgca gatttcaatc gctttcttct caataactgc ctcttcaaaa gaaatgccct 38820
tggcctcgga ataggactta attttgtgac agggtttgca gacgagttgg aagtttacac 38880
ttgtttgtcc tccacagtgc cataagaagg attctgcggt ttccttagat gtacatcctt 38940
ctgattctat cacatgatca cattctagtt tactcccagc cattacggtt ttacagaaaa 39000
cacattcccc tacatttttt gttgagggat gaaactttcc agattgcttt tcttctaaag 39060
ttagtggtcg aagtgaattt ttcttccaaa ccttcctaag tgggtaatcc gaccaaagtt 39120
tacgtaaagc ccctcttaac cagttccaat attgcgcctc cgttttccaa acaactttac 39180
cttcttcact ttcccaagga ttgttcatta taacaacccc gcatctttta atgacaacat 39240
ttcttctaca gacatcatag attttagttt ggtaatttta tctaatggat ctggtgcaaa 39300
ctctaacact tcaggaactt ctttgtattg taagttgtgc catgtagata tgattaggtt 39360
atccatatcg taaatagttc ctgtagcttc gtcatatttt agaataactt ctttcattaa 39420
aaatgttcct catatttatc ccaaaactca tccgattgta attggttact cagagaaaag 39480
atttcagttt gccaagttcc tgtctgtgtg tcaagattta ccattaaatg ttcatcatct 39540
ttgatataac cttctgggac ataacattga taacctacta agtattcttt cttatcattg 39600
ttccacgata cccaactgat tgtatggtct tccgtgaaag accaatgaac acaatcggag 39660
attgagagta gactagggta gctctcagga agactgtcat ggaacttctg aagtgaatct 39720
aaatccatat ttatttctcc ttatttaaaa ttcccacaag cacactcacc ttcacttctc 39780
gcttgacagt aacaatcttc attttcataa acatcagtga ttgtgtagtg tttatctttg 39840
tttaagtgat caagagtttt attacggtct tcctctgata attcaataaa tgttccataa 39900
tccatataat caccatcttg gtaaatgtag tctatacaat aattgatcat ttatttctcc 39960
aaattcttct catgctggac atactcgtaa tgacattcaa aatcaatacc ttcaagctta 40020
gatttgattt tatcaaacac ttcattcttc tcaagagatt tgagctgttt ggcacctttt 40080
aagtcttcgc tcaatataga ttgtatgagc gatgatagct cttttaggtg atcttcattg 40140
ccgcaatagg tctttactgg cggtgacaac aagttgttgt tttcaatctt acttgtatca 40200
accttcatgg ctttgcatag tttgtagaaa tccatatcat cttcataact acgggtcatg 40260
taagcaactt taaaataggt atccatgtat gtcatagtat caacatcaag gttttcattt 40320
ttatggttaa catattgaac accataaggt agaagttcag aaaatttatc tataacagct 40380
tgtaagactt ccttagctgt cttacaaggt ttgagtaatc tgtaagctga ttcatcaccg 40440
aaattaagat tttgcccaag atgcgaaaga caattgtaat tatcggcacc atctccagaa 40500
cacattgctt gccacagaag tcccttaaag ccatagaatt tataatcaac tgaacttgtt 40560
ttctggatta tccctatatc accacaatcc tttgttgaat ctttaatcag tattgcatta 40620
gggaatttaa acttacctct taaagggtta ttctctccaa cgtaagtatc aggatccact 40680
agtaatttgg ggttattttt agcatctttg tctgaagcaa taacaatatt atcgaatttg 40740
ccacatttta gataagataa gtagcctatc caaccataat gttctacacg atcatctgtc 40800
tcataaccct cacgggcaac ttcacaatca agttctttct caacccactc gcgaatctct 40860
ttaagtataa ttgggcggga actttcatta cggttaccct tgtaaggtct tactaaatct 40920
agttcgtgac ggaaacattg cccagatcca atgtgcacct tgatttttgg tatcctaaac 40980
tgttgacgga tctgtttaag tttcatataa atttgaactt tagcttgttc cacagccttg 41040
ctatgttcca tcttaagtct tgcagtttca gttatttcga aatcttcaag attccaagga 41100
tctttaccct caagctctcg ttcaatgttc ttcataccta accaactttt aggttttatg 41160
cctgcaccta aacctttaaa cgtagattta ttcttaaatt cctcttccca cccctctgtt 41220
ttatgcttga caataataaa cttattttcc atgttagcac aagccttcca cactggagta 41280
tcgaaatcgt aatgacctat acaatcttca gtgataatat cacgtatatc cttcacttga 41340
atttctggga atagtttatt atcactttca tctcggataa attctttctt ttcttgttca 41400
ggttcttcca caacattact agatgatgta aaacctttat tttcaaatct cattatttct 41460
cctgttgaag tcttattttt gatagtatgg atttagaata accatcctct gcactctcta 41520
agaacagtaa tccatcttcg aatgacctgt aggttttctc tcttggtttc actgttctaa 41580
aatctactcc ttctgcgata agagcttctt ctgagttacc atacttatta accaagtgac 41640
aacgagttcc atagctatat cccagtttat cccataaatc ttttgcggag tatacatctc 41700
ctttatactc aaattgccac atacgatcct tgaattgtac agacatcaat ttttcaatat 41760
cttcttccca ccctaaatga acaacacgat cccatatcag tcccttttca agacctaata 41820
agtggttcca ttcttctatg gttaaagtaa ttccgtaacc ccttatagta tgattgaatc 41880
tcgtgttgtt tacttgagtc ttcctattca cccatgaaca attactaggt tcataatctc 41940
cattggtatc caacctctcc agttctaagc ctattccgaa agatgattcc atatcaccaa 42000
gaaaattcaa aaacccgttt ttggtagttc ttctccaaga atcacaaaca gctatacctc 42060
gtcctccata gtgtttataa tctttccttg ttgaggtata acatcttcga ttcatatctg 42120
tccatacact atacagaggg tggtcagtga ccctttttac aggaaacttt actccacagg 42180
atatccctcg actacaacca caacttgtag aatctcctct ccataaagaa tctacacgga 42240
tattcttcgt ctctccgcac gaacatttta ctgtcacata tctataagcg tttctgccgc 42300
gctccttgta atagaaaggt gcaatcacgg ttaactctcc gtacacttta ccaacagagg 42360
atagcagttt ttctgtgaac ttactcatca ctcacctcca actctctcat tacctcaacc 42420
aaccactccc tgataacttc gttatccatc acaagaaatg tcccttcttg gtttgtcacc 42480
aaatggttga tcacttcatc aatcttgaga gtaacctcac cactaacctt attgtgttta 42540
atcatacagt ctccttttac acaagtatga aattacattg tctcaaaatc aatgaagttt 42600
tggaaagaat taaaagttat atgatataac taattcttag gtggatgata ccaagatgtc 42660
tctacaccat cgaaggtttt gaacttgaca ctagctacag ctttaccttc ttcaatattt 42720
tcatcaaaca gtccaaacat tactttttca atctgaacat taccgtaagt ttgaatatca 42780
caaaccttct taacttcttc gaagtctaac catagattag gttcaatatc taacattaat 42840
cctccttcaa agaatcatta actgattgca ttaaactgtc aattaactgc tccaatgtcc 42900
acccatcatc aagaaggctt tggtaaagtt cgatatctgt cccaagataa tatttaattt 42960
tctcattaaa ccaattacct tcttgacctt ttacaaagta gtgtttcata tcacctccta 43020
aataataaac agcatccaag aatactatat ctattcttac gattcaagga tgctttatta 43080
attattttac ttgtttacgt gccaaatcta cataatcaag aatattctct aacgtcttaa 43140
cttcttcaga caccttacct tcagtcattt cagccaccat cttcttgatg aacttggaat 43200
aatccccctt cttagcttct tccgagattt tagttttgta aacttcgtaa ccttgatcaa 43260
gggacgcctt aattgcatct tgtgcagatt ggatttccaa tgtcaaatcg tagacacgtt 43320
ggagggtgtt aaccattgtg cttgcatctt gctctgggtt tacgaattta atttgtactg 43380
tttcttgttc gatcataatt atttctccta ttaatgtctt tacttattga actgactatc 43440
ttgtgtaaat ttatatagtg atactacttt catgctttct ccttatttct tatctataag 43500
tgcataacct aaaaccaggc taataaatcc tgagaccatt tgaattaacc agtaaaatcc 43560
attactaaac aatactgtaa agaatccttg gtcggttgta aatagttcat aaataccgtg 43620
accaatccaa actacgttac ccgaaacaag aagtaaccac cccaaaagca taaaaattgc 43680
tacaaacata actttctcct taataaataa acttctctag acctacgggc tcatgaaata 43740
aacttgattt tatgatcttc ggcttatcaa atactacacc actttctaaa tctttaccag 43800
cagtaaatac gtaataatta cctactttct tatagcttac atctccgtaa cgaccttttg 43860
attcaataaa acctacttcc ccatccaaat caaatccgtg gtatagctca tcttcatgaa 43920
cgtatttact ataattacta tcagatatta cacggaaaac tccttctaca tccataattt 43980
ctgacatctt aacacataat gcgaaaagat gttcatgtag ttcataataa tcttccacat 44040
taataaggga tgaacttaac aaactgatca cttcttccgt ggtgaaaagg ttcttccatt 44100
ctttaaggtt tacttgaaaa tcttctggac tgtctgtcaa ttcacaaagc atccctactg 44160
taaagagtag gtcccctaca ccatcggctt gcatcacttt atcaccagta aaccaacctt 44220
gcaagaactc attttgacca aacatttctt cacgacttag ttgtgtgtag acagggataa 44280
gtgatttatc ttctacttca ttaccaccaa ttttattcca ttgcacaaca tcttgaataa 44340
tagcatcgaa tgtattacgt gtaaattctt tcttctccaa cctacttctc ctctaacata 44400
actaattcat tttcaaaata atcttcaaac ttactgacaa gctccttgtc caatccttca 44460
tcattaagtt tccaaatgat ttcatccttc atacactcta cttgttgttt ccatatatca 44520
acaagaatat atttctggat atgtcgatca cataagtaat aaatagtaga ttcatttgta 44580
gcatcacaat tatcgctgtc ccacacaaat ttaccaaaag ctttcacgtg gtgaacatgg 44640
tcttgccaaa cgtactctac tggaaggtaa tgttctgttc catactggtc attaatcttg 44700
tctacaatcc gtgtaaacat gtcgtggttt agcataaact gttttcctct ttcttacgat 44760
aatattcatt ttgacgttta acactttcta cgaaacgtaa tacatcattg taattgacag 44820
cttcaactcc catagaaccg tgataagagt attcgtcatg ttcttctgct gtatagattt 44880
caagagaatc ttcatgtaag cctaaactaa aacaggttac actaaaatct aatttttcac 44940
aaactttata taagtcttga ataagacaaa gcatatcaca tctcctctac ctgatctagt 45000
ccaacccaaa ctctattttt gttcaaatct aaaactactt ccaaccaagg gtatccttca 45060
cttgtgtcaa ctttacctgt tacaccagtc ataccttcat ataagcctgt taaaatttta 45120
actttcatag tattcctcca cccaaccttc ttcgatatct atgtaatcaa accacatatc 45180
ctgtccagcc aagtccttac caaacataaa cttctgagag ctcacacaga aatctgcagt 45240
aacaacttga ccatttttaa cattagaagg tttgttccag atacgttcaa tattattaat 45300
taaaaattca cctttcatag cacctcctct agatatttaa caaaaacttc ttcaacatca 45360
aaccaagttt tacacactgt atgtggtttg tcaagctcaa cttcttgaac ataaggcgat 45420
tcccacttaa taagtttcac atcatcagac atttcccgta gaaagatatt tctatcatca 45480
atcaacaaat cacatttaat aaggtgcttc tttctggcag ggatgaaatt gaaatcttct 45540
ggtaacatga aatcaaattc ttccttcaat cgtttaagtt tgtactttat ctgatcaggg 45600
cagttcatgc aataggaaat aaaaattatt tcacaattag cttcatagag gttacgaatg 45660
acgtcttttg cgccttcgtg aatatcacaa tttttataag tactcggatc gctccaatac 45720
ttaaaaccat cttctgatga aataccttca aagtacttag ttagattata ctctacttta 45780
ctgttgtttg aatcaccaat atactgttta tggttaaaat ctttatttgg tttaagtatc 45840
aaccacttaa gccggtgaat gtctgtgtta aataatgtta tgtcaagatc tataccaatt 45900
ttaattttct gcattcaacc acctattaac cagaaactca acatcattca agaaaccttc 45960
ttctgttccg ttattcacta tttgataaaa ttgaacttta tcatcgaaca tttctggtgt 46020
caaataatct cgactatctc cttcgaaaga acaaccttca cgttgaatac gtatgactaa 46080
aacattttct gcaccaatat gatcgataac tggttgtagt tcttctggga agccagaatc 46140
tgatacaaac acaatacctt cttttggaag tgaattcaca aaagctttac caaaagcatc 46200
tttaccaaat gaaggtttga tcacattttc actcatatgt atcaggaatt ctcgttttga 46260
atactgcttt cctcgaatac gctgggagat agtccccgta taatctttat accacatatc 46320
tggatcttcg taaaataaga gttccttacc tttacgatta taaacttctc ccagtgtctc 46380
atcataacca tctttgaagg tgtttacaga gacacctagt gtgtttacac agatcttgaa 46440
tagttcatct ttgaatgctc gatgataact attagtcctg atcttacaca tatgagatga 46500
tgcagaatct ttaccacttc tggcgggacc attgaatact accaccttta acatattaat 46560
cctcctcttt aattttacct aacaaaatct tcgctgtatg agccttagat ggtgttgtat 46620
attcacccat acgaacgtat tgctcaagca ctttagcagc attagaatag cttgtgaagc 46680
cattctcaca agtttccaag ggtgttttcc ctttaagctt acctttcttg taaggcgttg 46740
tgtagtgttg cacaagttca tatgctttgt caaaggttag atttgtgttt aaattaatca 46800
tagtagttcc ttattaatat ccgtgattgt ttgctgccat atcttttaca gtgatgtaag 46860
ttagtgagta atcagatggg ttgtagtaaa gcaagtcatc aatctcggct tgagattcgt 46920
tgataacacc tttcttcatg atgtgaatct ccgattgaca accgttgtca ctattaaaca 46980
tttcatgtac aagaatttca atagttttag gtttcttttt aagacacctt tgtagtgata 47040
acacccactt acgttcttct tcagtcaact tattcatctt taattttctc cttaacaaaa 47100
tcaactaacc ataaatgtaa aggtttatca atatcaacat ctagataacc aagttctttt 47160
gcgataaaca aaagagattc atggtcttgc ttataataca gtaaatcttc aaccagcaca 47220
ctcacaggta actttgacaa ttcttctttt attgttggtt tcatagtttc tcccaccaat 47280
tagttttaat cttacaatta cccatctcaa ctccacccca tccatcggag cattcccaac 47340
acacaatcat ctcatctttg ttgatctgct tgaatgagtg taccacgccc ttcaacattg 47400
tggtaggtgt ttttactact acactcgttg ccattttcaa caagagaacg cataagtgct 47460
tcacttaaga agtctgattc aaccttaacc catttactca tagtccctcc aattgttcta 47520
caattatccg tagtgaaggc cgtaaacttg ctgactctcg tgcattggcc caagacgtta 47580
acgtatccaa gatgcagatg aatactgcta cacgtttacg tgatgcactt gaaactgccg 47640
gtatcaatat tcaagaagtg ctggttcaag atattacatt tgatcctcgt attgctaagc 47700
aaattctaga tactcaaaaa cgtatccaga acgaagaaca aaagaaatct gatgagcgta 47760
tcgctaaaac tgaagcagct attaagaaag aacaagctat cggtgaaggt aacagaaagc 47820
gtgaagcagc agatgctgaa gcttaccgtg aaaagacaga agcagcaggc cgtaaagaag 47880
ccgctatttc tcgtgcgcaa ggtaaagcag agtctattac tctacaagct aaagcagaag 47940
cagatgctat taaactaatg gctgaagcaa accgtgactt ggctagatca atcacacctg 48000
cactacttga atacaaacga atcgagaatg aagcaattct ttactcgaaa tctcgtggtg 48060
aagtaccaac aactattatt ggtgatactg atctacgtgc aatcggtgtt cctgtagcaa 48120
ccggcactaa ataattgaac cagtccctgc caaatggtgg ggactctttc tttggattat 48180
tcgataaggg aggacttcgt cctcttttat gaaataacct caagaaaact actaaaggag 48240
tgaaccatgt ttcacaattt ccaagaagct gtaatgaaac aaatcgaaaa gatgtctcgc 48300
tttccactat ttgaagtgga ttactctcgt gaggagatct ttgaaaagta ccttaactct 48360
ttccgtccag aagataaccc aatcttccgt gagcgtactg aacatgattg ttcatgctgt 48420
aaatcgttca tccgtcaaat gggtggtatg gtcgctatcg acaaagataa agtgatcaca 48480
atctgggacg ttaaaggtct tgaaccaaaa taccaaatcg ttgctgatgc aatggctctg 48540
tatatccgtg ccctgcctat caaacacgtg ttcaaacgtg ctgaagcaaa aattggcaca 48600
aagcaaaacg tagaggtaac tgaaaatggt tcaattactt gggatcattt ctattctgaa 48660
gtacctatgt ctgcccgttc tcgtgatggt gatcgcttgc gtggcgaatc cgcttctcat 48720
aaagatagtc tggaacgttc tattacagaa ctaaacccag atcatgtgga agaattggtt 48780
cagttgtgta aagacaacaa cctgtactta ggtagccaac aacttcctaa actacagaaa 48840
cttcaatctc tgcaacgcga atacaatgca gctaagaaca aggagctatt cctatgggta 48900
cgctctgtgg agctgggcgc tgcgtctact attcgtaact ctgttatcgg tcaggttcta 48960
gtgaacatgg ctaaaggtga cagcctagag actgctgtag gtaaatatga ggctatgaca 49020
gccccatcta actacaaacg ctctaaagct atcgtatcgg aaggtatgat caagaaagct 49080
caccagacag tcgtacagct tggtctggaa gactctctac ttcgtcgtca tgctactaaa 49140
gacgacatca caatcaataa cgtactttac gctgataact ctatccgtga atcgcttggt 49200
gcattcgata tgttggttgg ttcaacgagc cagtctacag aaatcaaagg cactccaacg 49260
cctatgggta ttgagcagtt cttagaagaa gtactaccta actcaagcaa gcttgaagtc 49320
tacatggaga acagccactc agctaaccta atgagcctag tagctccggt taacgctgat 49380
gcgcctaaca tccttaaatg ggacaacaac ttctcttgga cttacaacgg cgaagtaacc 49440
gactctatct cagagcgtgt taaacgtgcc ggtggtaagg taacaggtaa attgcgtgtg 49500
tctctggcat ggcataacag tgatgacttg gacttaggtc taactgaacc aacaggcgaa 49560
catatttact tcggtcactc acgctcacgt caaggcggtc gtcttgatgt agacatgaat 49620
gccggtggac gtatgaaccc tgtcgatcca gtggaaaaca ttatctacgc agactctgtg 49680
aaagatggtg tctaccaagt caaagtgaac caattctcta aacgcaacaa taacaacgac 49740
aactgtggtt acacaattga agttgagtac gaaggtaaga cctacacgtt tgagtctgcg 49800
gctcaaatgc accaaggtca gacagaccta gtgactgagt ttgaagttaa gggtaacgag 49860
atttctttct ctggttcacc aagctctgct tcgaaagaag tatggggcgt cgctactaag 49920
cagttcacta ctgttaaagc agtatcacta tcacctaact tttgggatga gaaccaaagc 49980
ggtaacaagc actatttctt cttcttagat aattgtgtta acccagaatc agctcgtggc 50040
ttctacaacg aattcctacg tgatgacttg tacgatcacc gtaaagtatt tgaacatctt 50100
ggttcaaaac tgaaagctga atactccaac gatcagatgt ctggtcttgg cttctcatca 50160
accaagcgca acaaacttgt aatgcgcgtc gataaccgtt tatacgaggt acagttctaa 50220
tggaactatt cgcaaaagca acaaaacaac gtttacgttt taaaattggc aaaggtacta 50280
tctctacaga agacctttgg ttcattggca tggtcgagct agataaacta gccgttgctc 50340
tacacaaaga gttggaatct caggaaactg gttcattcct aaatcctaag aagactgacg 50400
ctaaaactca attagagttt gatgtagtta agtacgtact agaccaacgt ctagaaggtg 50460
ctcagcgtgc tgagaaagca gccgttactc gtgctaagaa cgaacgcatc gacaagatca 50520
tccagcgtaa acgcgatgaa gaacttgaaa acatgtctgt tgaagagttg gaaaaactca 50580
aagcagaata atccctaacg agagaggcct acggcctctt ttatgatttg aaccaaaagg 50640
aaactacaat gtttggtcta tttaagaaaa aagctgctgc tgcagcagtc actctacata 50700
aagtagaaaa caaagatcta atggaagcta tcgtagctgg tgctgtactg gttgctaacg 50760
ctgacggaga agcttcggct gaagaactaa ccaagctatc aggcatcatt gagaacaatg 50820
acaacttgaa gcactttggt tcagctatcg gtcagaccat cgacaagtac atgggtatgt 50880
acgagtctgg tcgtcgccta gctaagtcta agctgatgaa agaaatcgct gatgttcagg 50940
cagatgaaga tcagaaagtt gaagcattca tcattgctat cgagatcgct gatgcagacg 51000
gtgagattga tgatgcagag atggctgttc tgcgcgagat tggtaaagtc ttgggcctta 51060
acccagacaa ctacatctaa tgcaggagcg tttactcgcc ctattccttg gactgctatg 51120
tttactagta gtcatcgtag atttcacgag caagatcctg tctatcacgg tagacggggt 51180
tctcgtttgc ggaatcgtcc tcttgatctt tcgtctgcgt aaacttgatc aagaaccaaa 51240
ataatccaat aattcaaagg agagactcat gtctcaagaa caagcaatca cagtaacatc 51300
aaaagcaaca gcatctctag acaaagtggc aacaagcgta gcgaaagcag tagctgacct 51360
acaatctgta actgaagtac acggtgacct tctaggtcaa gtggctgaag cgcaacagaa 51420
cttgaaccaa atcgaagaag atactgcaac taaagtacgt aaagcaaaag ttgaacttga 51480
ccttcgtgta actgagcacg ctgatggtgt tctacagaac cttctagctg agcgtggcct 51540
aacttctatc gacgaagatg cgctagttga gctacgtcat aaagctgatc gcacttcata 51600
cgaagttgat gttgaaatca agaaagcagt taatgcagca cttgcgaaga aagacactga 51660
gcacaaactt gaacaagcag aaactaacca agttaatgca gtacgtgaag ctcaaatgga 51720
atctcgtatc gagtctctag agcaacaact tgaagcatct cgtcgtgaag ttagcaacct 51780
aactgaacaa ctatcagcag aacgtgaagc tggtacagag cgtgcaaaag cagcaggttc 51840
aatgactatc aatgccggtg gtaaataata gtcgccatgg tagggggagc tacgctccct 51900
tttccgaacc ggagactcgg atgagattcg gtatataaaa cggggttgaa ccaacctagg 51960
gcagatgcaa aaggctttgt caggccagct tacatcgttc agtcggagga ctgaattgaa 52020
ccaataaact aagcgaaggg ggacgccaac cccacgacac taactactat tcgatattct 52080
ttctttatgg gacattccag tggcttaaat gcagcatgga attgacaggg catagctagt 52140
cgtagagggc tgatcacctc gtagacacat taccccgacc gtgtagcaat actagttcaa 52200
gcagacgata aaaacagaga gagaataatg aatagccgtt gacatggata ttctgaaata 52260
tgttactcat attttattcc ttatgaagca gcacttcctt gtgctcggca agtacatgct 52320
agaaattggt gtacagggta ggtgagatgc ctaccgtcat tctgaatgtg ttttgaacca 52380
gaatacatcc agaatggcat tgctgttcag tgacattttc taatgtctcc aaagttggaa 52440
tatatctatt tacctcatag tagatcggcg agagtcgcta gaaactggac ttcgattcag 52500
gtgggatgcc tgatacctat tcactacaac acgtcctgat agtctgtcgt tgtagcctgc 52560
cttcgggcag tcggcaagcc gatgctagaa attgatcgga tgagtgagtg agagacttgc 52620
acctaattta actactggaa tttgaaccat gaaaaaagta acccctgaag tggctgaatc 52680
attgaaagct attactggtt gcccaagaag aactgaagac atcgttaaaa acttcgacat 52740
tgatcgtatt tcatttttga atcatattcg atctgctcgt gcttcgggta ttccagtaga 52800
acatattggg ccaatgtccc atcgttattt ctatctccca gccgaatacc gctataacat 52860
caaagaaaca gttgcagcat caattaaaaa gatcatggcg aatgctcggg aagaggatga 52920
cataactgtg gaatacctcg cacaagtatt ggaagtaacc actaatcatg tacgttcagt 52980
attctatgcg tggaagaaga aaggccaagt aatttacagt gcacctactg gtgttggtaa 53040
acaaaagaaa tactggacag tgcctaccga acctgttgaa cgagagtctt gactctggtt 53100
catcgcaaac tctgcgatta ataagtgatg agtgaagtgc actgagagag tgcacaactt 53160
taattaactt agaggaatag gcatggcaca agatacctta gctaaaaaga agcaagcgta 53220
tatcctgttc aaacaaggtt ggactgtgaa gcaagtagcg cagcatatga acgtaagtac 53280
tcgtatggtt taccgctaca aagacgaata tgaattacat actctccgtg agttccgtga 53340
tcaattcaaa gttgctctgg aaatcgcaga caaacaacaa cctttatctg tatttttgca 53400
gatcttaaaa gcacaagcta ataaggaaaa gaacagtgaa aaatccgctt aaatttaaaa 53460
cagtagatga tgtaatgtcg gatactgtcg gtcaactaca agagatcgaa gaacgccaac 53520
agcgaaagtc tgaagcgtta gaaaagcaaa ttgaaaagtt gaaccaacag aaggaaactt 53580
ctgacggtga agttagtaag gccaaaatgg gcattcgaaa ctttaccaaa ctatttactc 53640
aggaattgcc cgacaacgaa gagtaatgac aaaacctaag tctcctgttt aaggggactt 53700
tatttttgtt attgctaacc gcacctctat gctgaaggtg gaaaggttac agttggctgg 53760
gcaactgaga catgcaagtg aaccacctta gtagttttct tttacttgcg tgaccccagc 53820
attattcccg aataaggcgg cttatccttt aatcgtatgc taacacgata tatgccgctg 53880
aagtccccgt ccaagagtag taatcttgcg gggcaaccta tttacacaaa cccccctatg 53940
atctgaatac gccgggttaa tttgttttgc cactgcgtta actaacgaac acctaaacat 54000
gaaaactgag gtgatcgtct atggtcttta ttcagtaggg gttttttatt attgaggcag 54060
gaacatgtct aaagaacttg aattcgattt atttcgtacc tccaaggaaa cacaatttcc 54120
tgctttagta gatttgcagg tactagaccc taattcagca gaaaagctaa tcaacaattg 54180
gttcaatact tcccagtcaa atgaactcct agcgaaagaa atacgtcaaa atcgtatgac 54240
gatgagtcat ttgaaccaag ttattgaaga acagaagcga gctatcaaga aagaaggtat 54300
agctggctta gtgatcggag ccttaatggg tgccgccact gtttctttac tagttatgat 54360
ctttggggga tagaatgcga tcgtttaatc aaaaagagat tgaagacttt ttgaaccaat 54420
ctcctagtaa ggcatacaag ctagtgcctc accaatttaa ttctagtcat gcaggcaaag 54480
cttgctgtac taagtgtggg ctgctcgccc ttcgaaatag agctaccgac tggtgtgttg 54540
aaaaaggatg tgagtttgag aaccaccctg attacaccaa tgcaatgaaa cgtttaacca 54600
aataaccttg agagaacaca atgagtaatt tagattttgc agtaactcca ttgaaagcaa 54660
tcccattgat tgaacgtgca atgcgcaaac gtattgtacc aatcctgaag ggttcaccag 54720
gtatcggtaa atccgatatc atgcgtaaga tcgcacgtaa gcacggtctg aagctagtag 54780
atattcgtct agcacaggtt gacccagtag agcttaatgg cttccctaac ttcactgcgg 54840
acggtaaagc ggtttacgct ccaatggata tcttcccaac taagggacaa gagctaccac 54900
ttctacctga attcgaagac aagaaaggtg aatatgatgc cgtacttggt tttggttcag 54960
aagaagagat caaagaattc cagaaagagt actgttacaa aggttggctt ctattcttcg 55020
atgagattac ttctgctaac cgtcaggtgc aggcagcagc atacaaaatc atcctagacc 55080
acttggtagg ccaattcgat ctacacccta atgcactttg tgtagcagct ggtaacctgt 55140
cttcggataa agctgttgta aacccaatgt ctacagcact tcaatcacgt atgtctcact 55200
tgactctacg tgttgataaa gacgaatggg ttcattgggc aatcgagaac aacgtggatg 55260
ctcgtgttat tgcattcatc gaataccgtg atgatctgct tcacgacttc aatccgaacc 55320
atacctcaga taccttccct tgtcctcgta catggaaatt tatgtctgac cttgtggaag 55380
acgatgaagc aatctctagt tcactaatgc cactattggt tggtactgtg ggcggtggcg 55440
cagcaggcga gttccgtacc tttacagaaa tctggcgaga attgcctagc cttaaagata 55500
tttcagtaag ccctgagcgt actacagtac ctaaagaagg cgctacctgt tacgcaacag 55560
taggtatgtt ggcatcatct gttgacctaa catcaattga accaatctgt aaatacgttc 55620
gtcgtttggg ttcagagttc caactagtgt ttgttcgtat ggcgttcaaa tacaaccgta 55680
ctctaatcca aaacccaaca ttccagcaaa tgttgaaaga cttccgtaag gacttaaaag 55740
acgaggaata aaatgtttga tcgtgataag gctctaagta aagctaaagt tgccattatg 55800
atgaaaccga atactgcatt catttcatct gtattaatgt cacttaaatc taagtgggat 55860
gataaacatg ctacggctta tacagacggc ctaagcatcg gcatttctac tcctttctgg 55920
gaaagactaa caccggggca acgagtattc ttgttgctcc atgaaacttg gcatgtggcg 55980
ttttaccatg taactcgctg caaagaatcc aagaaatatg aaccacagaa atggaattat 56040
gcctgtgact actacatcaa cttgatgttg gtcaaagaag ggtttgaatt catcgaaggt 56100
ggtttattgg actacgccta tgatggtatg tctgtagatg aaatctatga tgcattacct 56160
gagaacctac ctaacaacct gttagatggc gatatggtag aaggtggttc aatggaagaa 56220
gaacagccta cacctgatga cgttaaagca cgtatcactg aaactctatc aagagcaatg 56280
atgcaagcaa acatgtcagg ccaagctggt tcaattcctt tggatattcg acgtgcagtt 56340
gaagagatcc gtaatccacg attaccttgg aatgttgttc ttgagcaata tcttagtgat 56400
cgtatccaag atgaatattc ttggaacaaa cgcaatcgtc gtttctccca cgtgtatctt 56460
ccggcgttag actcagaagg tatgggtgag attcgttgtt atgtggattc ctctggttca 56520
gtgagccaga aagaactatc tcttgaagta aacgaaatgt gttacatcaa agaggtatcg 56580
aatcctacat taatgacact tcgtgccttt gcgtgttctc tgggtaaagt acagcagttt 56640
gaacgagatc aagaaatcga gttcgatgtt gacgtaggtg gtggtacaag tttcggccct 56700
atctatgacg acctgatgga caacccagat actgaagtgg ctgtaatctt tacagacggg 56760
tgcctaaacg tacctgacac atcggccctt ccatgtgacc ttgtatgggt tatcgtgaat 56820
aaccctactt ggacgactaa cacaggccgt gttattcaca tggagataaa acaaggagac 56880
tagaaatggc acgtaatccg cgtaaacgta gtgtgtcttt tcgaaactac gccctgcatc 56940
atactaagtt atgttgcttt tccaaagttg aaccaaagca gctagatctc gtggtgaaac 57000
atggcgcttg ttcatacaaa ggattagact ttgcgactcc ttggcattac ggcgtgattc 57060
caccctgtga tgaagcaaag tgggggcgtg atcttgaaca acttgatcac atctggttca 57120
taactagcga atggaagaag attgaatttc atgtcattca atggttgaac cactgcaata 57180
gtatggtaga catcgttaag ttgttgccag actcaattac cgataagttt gatacaccta 57240
ttactggtga gttaaccctg tcggacgagt ggatagacat ggttcaaaac tctgatgagt 57300
ttaatatgtt agaaacactt gtgttcacta acgagatctt ggagcaatga tgtgccatac 57360
tatttggcct tcatcaaaca gaggtctata tgcgccatct tattcattct gaaaactacg 57420
atatggcagt actcattaaa gatgctgccc ttcgtaactc tgaaattgaa aaacactatt 57480
tgaaccaaaa aggattcaaa gctatctcct tctcacttga ttatggtgga aagaagaagc 57540
cgaccgctac agtggtgaaa gattatcttg atacgttgct tccagtcctt gagcaatgtg 57600
gagtacgaga tctgcttgta tgcgatacgg aatatttcaa aaagctttgt aaggttcaaa 57660
aagctgatcc tcattatggt tacattctgc cagtagctat tcccggctac gagcacatga 57720
atgctgtctt atgtaccaac tatcaagctt tgctattcaa cccgaatatg caagacaaga 57780
tagacctagc tctcggaacc atggaacagc acaaagctgg ttcatacgaa gagattggtt 57840
caaacattat tcagcatgaa gagtacccag atcagtatga tcaaattcag aaatggctcg 57900
atagcctatt acttttgcca gcccttacat gcgatattga ggcttttagt ctcaagcatt 57960
atgacgctgg tattgggagt atttcttttg cttgggacac tagtagcggt attagttttg 58020
ctgttgacta tgtgccaaca ataccccatg aaattaaagt gtggtgtaag aaagacaaga 58080
aatataaatc ccgacaagcg gtaggcaagc agggatacaa tatgcgtatt cgccaaatgc 58140
tcaaagagtt ctttatcaag tatcaaggct ctctgatctg gcacaacgcg tcttacgata 58200
tctcagtgct tatttaccaa ctctggatgg acaacatcat tgatcaagaa ggtatgcttg 58260
aaggacttga agttatgaca cgtaactttg aggacactaa gatcattact tacttggcaa 58320
ccaatagctg tgcaggcaac aagttaggtc tgaaagacca agctcatgag tttgcaggca 58380
actatgccga ggacgacatc aacgatattc gtcttatccc taaagatcag ttgttacgct 58440
acaacctgat tgactgtcta tgtacttggt acgtcatgga caaacactac gacaccatgg 58500
ttctagacga acagtacaat ctgtacgttg acctaatgaa gcctcatttg attgatatca 58560
ttcagatgca gctgaccggt atgtgtttgg atatgaacca agtccataaa gctgagaaag 58620
agttgcaggg tatctacgat actcacttaa atgcgttgat gggccaccca atcatcaaag 58680
agttcactca ccagcttcgc cttgataaga tcgaggcaga caacaagaag ctcaaaagca 58740
aggagcgatc ctatgaagag gtatctcacc ttacctttaa tcctaatagt ggtgtgcagt 58800
tacaacggtt gctctacgag tgcatggagc ttccagaaat tgactacacc aagtcaggtg 58860
ctccagccac cggaggcaag accttaaaga aactctctaa gatgacacag gataaagacg 58920
ctatctttat tttgaaccac ttgagagaat acattggcgt gaataaggta cttacttcgt 58980
tcattcctac atttaaggaa gcacccctag caccagacgg aatgcattac ctatttggtt 59040
cattcattct aggcggtact gtatcgggac gcttatcctc caaaaaccct aaccttcaac 59100
agattccatc tggttcaacc tatgccaaag taattaagaa atgctttgtg gcacctaaag 59160
gttggctatt ctgtggagca gactttaatg ctctagaaga taggattaat actctcttaa 59220
ctcgtgatcc aaacaaagaa aaagttctgc tagatggatt tgacggacac tcgttccgta 59280
cttatcactt ctggcctgaa aagtttcctc acattgatcc tgaagaccct gttagcgtga 59340
acaccattca gaaagactat gatgcagatc gtggtcgatc taaaccggtt cactttgcta 59400
tgcagtacca aggcacttgg gcaacactca tgaagaactg tggctttagt gagcaagaat 59460
ccaaggatat cgaagctaac tatcaagttc tttataagaa gtctttcgat tgggtacatg 59520
cccgaaccaa agaagcttca gacaaaggat acgctactgc tgctttcggc ctacgtatca 59580
gatgcccatt gctaggtaag accatattag gttcaagggc tactttgaac caagcggcag 59640
cagaagcaag aacacttggt aatgctatct caggacaatc ctacggattg ttaaatggtc 59700
gagcatcagt tgaacttatg cagaaagtgc gtaatcatcc taagatgaga acgtgcatta 59760
agcaatgtgc gcatattcat gatgcacagt acttctatgt gaaggatgat ctcgaagcaa 59820
tcgaatggtt gaatcgtgag ctaccgaaag ctatgagatg gcaagaactt ccagaattac 59880
aacatgaccg tattacacta ccggctgaat tagatatctt ctaccctact tgggcagacg 59940
gtttccagct agagaatgat atgactcagg atgaaattgc tgaagtttgt cgagcagagg 60000
ctcaaaaacg aagagagagt gaaaatgggt aaaacctaca aagatcgggc taaccacgcc 60060
ctacatcatg gttatgaacc aaatggtgat aaagagcaag agcttgctga tatcttagac 60120
cgtaatcctc acaagggtct acgccacggc aacaatcgta agagtgaagc caaatctaag 60180
aagatcgaac gccgcaaact tcgtcgccaa aaggagaatt tcaaccatga ttaaaatcaa 60240
taacaagcat caaaatgcta cgttacctca aactgtcgaa aacattgcac gctggcataa 60300
cgagcgtaac ctgatcgacg gaaccacttc gtataagcaa actgaaaagt tgcttgaaga 60360
attcaccgaa ctagtttcag ctcaattggc ccgagacacc ggtaagaaac cttcatctga 60420
attcgtttat gacgaaactc gctcaatgtt agaccgcctt tacgaaggtg gacgaatcaa 60480
aggaattgaa ccagaacatg ctgaagatga acttcaggat gcaattggtg acatgcttgt 60540
ggttcaaact aacattgcag agcgtgaagg tttcactatc tccgatgctg cattcaacgc 60600
ttacctagag atcaaagatc gtaagggtaa gatgattgat ggtacattcg tgaaggaggc 60660
agacctgtga tcaatgtaca aattattgct gactctaaat atggttcaaa tcgtgtaact 60720
acgtttgagc ttgagtatcc gcgatacgtg catagtgagt tgatgactca ccgcgtattc 60780
tcccgtaatg ctgcatcctc tcgtgctatt cccatcgaaa agatgatcga acaggctacc 60840
gagaacacgg ttatcccaat gtggtctaag aaccagaaag gtatgcaagg cgaacttgta 60900
cgtgacctag agactatcaa tgaagcaaac cgtatcttag gtgtgatgct caacgatgtt 60960
gtcgaaggtg tacgagagct aagcgaattg ggtatccata agcagaatgc taaccgctac 61020
cttgaaccat tccagcacat caaaaccatt gttactgcta cggaatttga gaacttcttt 61080
aacttacgtt atcacgaaga tgctcaacca gaaatccgtg cactagctgt cgagatgtat 61140
gctgcgtact gtaagtctaa gcctaagttg ctcaagccga ctgaatggca cctgccttac 61200
gttgatgtac cagtagctta ccctggttca gaatctgata gcagcgatgc tatttcacta 61260
gatgaagcta agaaagtatc agcatcatgc tgtgcccaag ttagctaccg taagaacgat 61320
acttctgttg aaaaggcaga gtttgtctac gatcgtctag taggcggtga accattacat 61380
gcgtctccat ttgaacacgt atgtcgtcct ctagctcaaa gcgaagaaca gcgtggtaac 61440
ctaatcggtt tccgtcagtt acgccagaat attgaggaca aggtatgagc aaatctgtgg 61500
tagatatttt acaactggct caagactgtg tgggtgatcg tgcggcagaa cgagatacag 61560
atgccgaacg tagcatgaag acgtgtattg atgcatttaa tgccatgtat ggcaccgagc 61620
ttaccgaaga acagggatgg atgttcatgg tgttcctgaa ggcttccaga gccaagggag 61680
gtaagttcaa gctggacgac tatgtggata tggcagctta tgcaggttta gcaggagaat 61740
gtcatggctc aaaagaagtt cagtaataat cacggtatca gcctaccctt agcggtatgg 61800
ctggcccgag acgagtatga tcatgatgat agaaccaatg tgatctcagc aactagtttg 61860
ttgaagtcgg ttcgtcagat tgtgctcagt acccgtgtgg tgccaacagc aggtaatatc 61920
gaggatattt ctgataggct tgcttcccgt attggtactg cgttacacaa ctcagtagaa 61980
cacgcatgga cggaaaatta tggacgtgcc cttagtgatc ttggttatcc tcaacatgct 62040
attgatcgtg ttgtggttaa ccctacttcg gttcaaaaga acgatatccc tgtgtatttt 62100
gagcaacgga gtgagaagac ggttggaaaa tggattgtct ctggtcagta cgatctcgtt 62160
tttgacgggg ttgttaatga tgtcaagtct acttctgtat atacgtggat aaacaatacc 62220
aacgacgcta agtataagaa gcaattatct atctaccgat ggcttaaccc tgagattatt 62280
acaaaacctc atggtgcgat ccagttcatc tttaaggact ggaacaaaaa catggcgatg 62340
ggtaaagata aaaaatatcc taaacttcct gtgatggaat acaaactcga actggatcaa 62400
ccatctacca ctgaacagtg ggtgacggac aaattgaacc agatagatga atatttacaa 62460
gtgccggaac ctgagttacc gttgtgtacc ccagaagacc tatggcaatc tgaaccaaag 62520
ttcaagtact attcatcgcc ttcggccaaa cgtgctacca aagtgttcga taacatggcg 62580
gaagcaagac gttacttaat ggttcaaaag aagggaatgg gactagtgaa agaagtatat 62640
ggtgaaccta ctgcttgtaa gttctgtcca tgtgccggta tctgttcaca gttcaaaggg 62700
tttgttaagc aaggtctaat taaataaggg gctatcgccc ctttaatgag tctaacatct 62760
aaggaataac atggaactca aaccattatc cgaggtggac tataatccaa ctcaagagga 62820
tttactccaa gtactgcgaa ccaaaacgca gaacatcgag tcagatctat actttcgtac 62880
aatcatcgct ttctatctat ctcagattgc ttcatcaatg cgtgcaacta ttgttacacc 62940
gcaccgaggc aaactaccag tgaacatgtt tgtatgtgcg ttaatggagt ctggtgccgg 63000
taaaggttat tcaatgaata tcatggaacg agaaatcgta cagggtttca gtgagacttt 63060
ccgtaaacag acattcccaa acgtatctga gcagtctatt gatgacgaag ctcgcagacg 63120
tgcagttcgg aaccaaactg atgaagctga agaacgtgaa gaactctgga aagagtttaa 63180
ctcactaggt gctatgcctt acagctttga ctctggttca ggcccagcct ataagcaagt 63240
gcgaaccaaa gcacagattg ctggtattgg tgccactaac ttcattggtg atgagatcgg 63300
tactaaccta agtagctgga gcgaaatgct tgcagttaac ttggaagctt acgatgttgg 63360
tttaatcaaa cagaaaatca ctaaaaatgg ttcagactct aaacgttatg aagaacgtag 63420
tgacccagta cctgcaaaca tgctggtatt cggtactcct tctaagctgt ttaacggcgg 63480
cattgaagaa cgtgaattca tttcactatt ggagactggt tatggtcgtc gattcctatt 63540
tggtatcggc aagaagactg tagctcagga tatgacagcc gaggagctgt tcgatatctt 63600
aacgaaccac aacagtgata cgctagttac ccaactggct aaccgtttcc aaacactggc 63660
tgacgagatt aattacaatc gcgagattaa aatctctaaa gatgtaacgt tgattaactt 63720
gcagtaccaa ttggactgtg agaaacgtgc atcttctatg tcggtatttg aaccaattcg 63780
taaagcagaa atgcagcacc gttacttcaa agcattgaag ctggctggtg tatacgcatt 63840
cattgattgc acgcctgaga tcacagaaga acaaatgtat gcggctatta agttagttga 63900
agacagtggt aaagcttgtg atcgtatctt gtcgcaggac aagaactatg tacgactagc 63960
gaagtacatt gcagcagttg gtaaagaagt cacatatgct gacctagttg aagaccttcc 64020
attcttcaat ggttcaaaag cagcgaaaga cgatatgatg tcgttggccc gagcatgggg 64080
gcaccgcaac aatgtgatca ttaccaaaaa gtttgatgat ggatttgaga tcttccgagg 64140
tgaaactcta aaagagactg accttaataa cctgatcttc tccgcatcga accacgttgc 64200
ctataactac atgccttcta cgcacaagaa tctcaaggca gtacgctctt gggagaactt 64260
ccatcgtgtt gctacccaag atggtgtgca ttggtgtaat catactttcg aggatgatca 64320
ccgagtagaa gagaaatcta ttcctgaatt caacatggta gttattgatg ttgatgggga 64380
atgttcgtta gagactgcac agatgctcat gcaagagtac aagtgcttct tctacacaac 64440
caaacgtcac actgacgacg agaaccgttt ccgcattgtg gttccactca aataccactt 64500
gaagatgaca gctaatgagt acaaggagtt catgaacaac ttgtttactt ggctaccatt 64560
caaagttgat gagcaaacga accaacgctg taagaagtgg ttaagtcatg caggaggcga 64620
gtcattgtat aacgatggcg aaatcctaga tccaactcag ttcatcccta agacagccaa 64680
aaatgaaaga cgaatggctg aagctcaaga cattggcgat atggataaag ttgaacagtg 64740
gttcgctcgc tctattgaag agggcaaccg caacaacact attttgaaat acgctttaat 64800
gctcctagac gggggcatgg cccctgacga ggctgaaacc aagactattc aattcaacga 64860
taagttgaag aatggtttgt ctaaagatga gttacgaaca acagtcctta aaaccctatg 64920
ggttaaggca caagaacaag ggaaaatcta atggatttat caaataaatc ttactccgaa 64980
ctggttcacc ttcgcgctca aatcaatcaa gcgattgctg agcaagaaac ctctgtcgtt 65040
gaacgtctga acaaaggcga aatcgtaccg aacttccgtt tcaaagaagg tcgtatgaaa 65100
cgtgcagtta cgaaccaagg tatgttggtt caaacgctac gtgacaaagg tctatctaac 65160
gaagacttgt acgaagctaa gttcgtgggc atcccaacgc tagagaaagt gcttaaagct 65220
aaactatctc aagcagatgc tgacaagatc atgaaacgtc atattgaagt ttcatacggt 65280
aatccaactc ttgagtacac aggtgaataa tggctaagca gaacgacaac ttaatcctcg 65340
tagtagggaa gtcggctgct ggtaagtcga tgtgtcttaa agacatcaaa gatcacgaag 65400
gcgtgatgta cttgaactgt gagtctggta agaaattacc attcaaaaac aacttcaagc 65460
gtctaacaat cactgaccct tacatggtat tccaagcttt ccagaaagct gaagaaatgc 65520
cagagattca tacgattgtt attgactccc ttacgtacct aatggatcta tttgagtccc 65580
tatacgtagt acctaaagct ggcaccaaac aaggtatgca agcttggtct gactacgccc 65640
agttctttaa agcattaatg caacaatatg ttgcagcttc tacaaagaac gttatcatga 65700
ctgctcacac tgctgatgaa gttaatgatg acatggtgaa agaaactttg gttaagatca 65760
aaggttctgt catgaacaac ggcgtagagt cttacttctc acaaatcgtg gcatgtaaga 65820
agatgcagct aaagcatcta gacgggtatg aatctgacct gctgaatatc actgaccgtg 65880
agacacgttt gaagcacaag tatgtgttcc agactgacct aacagcagac acgattaacg 65940
aacgtattcg tggcccattt ggaatgtggg aagaaaacga aatcttcatc gacaacaaca 66000
ttcaattagt actcgatcga attcatgact actacggtga gtagtagctg aaccagatta 66060
ccactatggg gactacgtcc ccttttgcca taagtaacac aggaaagaaa caatatgtta 66120
gacgcactaa aactatcacc agatgttgaa ctagaaaatg ataaagatgt acttggtggc 66180
ggtggcccgc ttaccacaga tatctatgca cttacatgtg agtacatgta tctagagcaa 66240
actaaatctg gtgcagttat ggctgtaggt agcttcaaag aagcagactc aggccgcaac 66300
attcgcttca gcgagtgtgt aatgtctaag aaatcaggta cgttgaaagc aacatacact 66360
gacaagaaga ctaagaaaga gcgtccgtta ccgggctaca ctaaggtact acaccttgct 66420
aaagtgattg gcctagacgt agctgactta tctggtctag cgtgtgaagc gaaagttctt 66480
aaactattcg acttcaaaga gcgtaaagaa gtacctcaag agaagaacgt acttactgac 66540
tttaccggtg cagcacttaa agcaggcgtc tacctagttc gtgaatccaa aatggaagaa 66600
agtgaaccag gtaaatggga tcagccgaca ggtgaagagt tcgctaagaa cgatatcgtt 66660
aagtggttca acgaagatgg cctaaccatc gaagaaatga ccaacggtgt tggtgatgaa 66720
ccatttgctg atcagtggcg tgaagctcac aaaggcaaag aacgcgataa ccgtgtgaaa 66780
gacgcagctc ctaagaaagg tgcaccacaa ctaggtggta ctgctccagc aggcggcggt 66840
cttaacttcg actaatgaat aggggagtag ttgtagctcc cctgcgcatt gctaagaata 66900
aaaagggtga taagcagtgg tcagtgaatt tgaaccaata ccggaataca cacttccaaa 66960
gtcttaatag cttgaagaag cgttataaag atctggtata tcttcaagta gatcaccttt 67020
caccctttac ttgtatccga cttaccattc atatctatgc cgccaataaa cgtctattcg 67080
atataggtaa tgttggttca atacacgaga aattcttttt agatgcactc gttgagttgg 67140
ggaaactacc ggatgacgat tacaatcatt gtcccgaaac ccatacgtac tttaaaggga 67200
tcgacaaaga tcaccctaga gtagaattta ttatcgagga aatcccgatg tcattatttc 67260
aaattgacaa gcctgaccgc atcccagtaa acatccgtac cactgaacat gatttggttc 67320
aattggtcaa agaagctatt gccaaagaag atccttctat cgacgtaact gatgttcgtc 67380
tagttggtct tgaagcgatc gagatcgacg catttgtagg cgaggctcct aagaagaaaa 67440
ctacccgtaa gaccactaag aaagctgaac caaagaaaga agaggttccg gctgaagtgt 67500
taaacgaagt agttgaagca caagaaaaga aagaaactga agctgaaaaa ccagttgaga 67560
ctgcggaaga tacagatcta ccgttcgacc ttgacgatgt tacagaccct gaagcttcaa 67620
gtgctgactt aacttttgcc gaccaagagc tatctctgat tgatgaagtt ctagctgaag 67680
actctaacga gggtgcagta gatgaaccaa tcaacactct aagcttcgac acaaatgaag 67740
aagcagaaag tgaaccagaa acaggtggta cgactgtagc tgatatcttt aagtaatcgc 67800
ctatgttcct gaccattccg tttaacccat tcatgatgct cgctttcatg gtgttctgtt 67860
tagtcgtaat cttcttcggc tatcgaatct ttaaatggtt cagaaacgga atgaaacggg 67920
cattcgatga aatagagaaa gaaaagaagg ggcattaagc cccttttttt attagttagt 67980
gaatggtctt agcaatagag cattgctgat catctctaac gggttgtccg gtaatcttac 68040
acgggctgct agagtatcca acgatagtag tgagtcaatc acacttggaa tagctccacc 68100
agtcattgca ttaagtacgt tgaagataat gtttcgtgca gagttctcac ggaagttacg 68160
gataatctgc ttctgaatac ggaagtagta cttagtgaac caaagtacac ccatatcatt 68220
caagtactgt agcaacggtg ttgtcggcat atcgtagttg ataaacacat ccataaccaa 68280
accagcagca tcacgacgac tcataccatc attctctgtc ttgtgcttgt atagcgcgta 68340
acgagataca aagtctgata gctgagctgc ttcattcatg atcttgaata gcttagtgtc 68400
atgtgacatg taaactacct tggcaccatt tcgaatgaat cccggtaagc gtgtagtcaa 68460
cgcttccact ttctgagtta actgtgactt atgagagaat gtatcatcag ccaattccac 68520
atcttctaca atcgactgca acataccatc ttcaatcaag cttccaacag gactagtcgc 68580
taattcgtga cgcagttcag ccatacgctc ttggttctta tccaagttag ctggttcata 68640
ccccaattct acaattttct ctaactcaaa taactcagca gcgtgacgac ggtaacgacg 68700
tgcttctacg taagcctctt tctgccactt caacgcatca ccaatagaca taccttctaa 68760
gaatagctga gaggtatttg atagagcatt catcagggtt actacacccg acttaactac 68820
cacaaattcc ttagtctctt taacaaattc tttccagata tcttctgctt tacgaatcgt 68880
acctgcggct ttcgggccta atacatggtc agcaaattga accaatgctt cacgtaccca 68940
acttgtctga gctttttcac gatcaagcaa gtcagcagca gatagtttac ggttaccgaa 69000
tacaatcgga gccaaatcac gacgaacata catacggctt acaccccaaa catcgcgagc 69060
atatgcttta gcatctggtg gaagagttgc ccagtattct ttcagaactg gatcttccga 69120
cttagccgat acctcaatgt atgcttcaga attagtagca tcgtattctt cgtatagagc 69180
atcaatcacc tgacggttca cttctggtgt tgtgcgttta tccaaagagt atgcagccat 69240
tgcgcccatt acatcatcaa agtggttatg cttcgctagt agagtatcct tagtctcttc 69300
attcatcacg taacggtaag agttgatcgt accatccgca ttgacgttag gaagtaactg 69360
accaccacgt ttagtagttg ggtcaactcg cttagcctga cgcatctcag caatatcttg 69420
ttgcttagaa cctttgatat ccattgggtc aaactcagtt tgagcagaac ctttcgcctt 69480
acgcgaagta agagagatga tacctgccat gtaagcatta gctccaccgt acttattcac 69540
cattaggtat accggttctg aaccaatgat atgagtatct tgttccagtt cacgtactac 69600
ttggtaacct tgcttttcgt attcagcacg ttcagcagaa ttcacttgaa ccacagagat 69660
attgttatct actaggtcac gggtataacc cttaatgaat tttgaaccat ctccatcaaa 69720
tagagttgct cggtcttcct cttgcatctg cttgtgcata tcaagaacgt aaccaacgct 69780
gtgctcgttt tcacgaatca attgaaccaa tgcagcttta tgctctggtg ccgtgtgttt 69840
gatagcgtac agtgaagcaa gcttatcaat cgtaccaaca tggttttccc aatccgcagg 69900
agtagttgtt acgcctagca tcttagcaat gttctcagca tttagcagag tgtgtgcttc 69960
gtacacgtta cctgttgcca tgtagtaacc aagtgcttgt gatgcacgat agtagaaacc 70020
acggttacgg cttgaaccaa gtagacctag agtacgagag atctctttct ctaactcaac 70080
aggactatca agtactgcct cgatttggtt catatctagg taatcccaaa gtgtagccaa 70140
gtcagtctta ataccggctt tagtaattgc tactttttct tcagagttca gtttacgctt 70200
aaatgaacca ttcagtgctt tagctgtatt cgtttcaatc tgcttacgat cgtggtcaag 70260
tagctttttc atctgacgag ctaaatcatg gaatcgtgca gtgctatctg tacgactcat 70320
tgcctcagtc aacagtgaac caaggatcgt ttgacgacca cctttagaat gcaacttagt 70380
ttgggccaag tattccataa agccaaacac accatcgtat gaccaacgca atacgttact 70440
tgctgtacga accactttga agcggttgtt cttcaagaaa ctcttgttac ccatttcggc 70500
agcagtctca cgagtctcat tagtaaactc gccaagtttg ttagccacaa cgtttaccgc 70560
agcatctgca tattgaacca atttgtgctt gttagctgat tcaactccag ccaattccag 70620
tgccaatttc tgaacacctt cagttactgt catatctgat gttaggccag acaagtgacc 70680
atttacccag ttcagagcag tatcaaagat attacgtaga gtctgaaccc aatcaccttc 70740
aacgaataga tctttagaga atacattctt aggcacttga gtacggctaa gtgcattcat 70800
gaacttctcg ttagtcaaag catacgctgc aaactcttgt aggtagttag aacgaccact 70860
tgcttgagca gtagtacgaa tgttcataac gtagttccaa gtgtctttag cacgcttgta 70920
gtcttctgag tccacaccgt tctgtgggtt atccattaag tcttcaacag ttacaagttt 70980
gttctgctta acataacgga acagtttcat tagctcacgt ttagcacgag agttaccgtc 71040
gatagcagca cgtaaaacac tgtgcgtcat ttcgtgagta aacgtctctt gtgcagacat 71100
ttttgaacca tggaacgcag ctacaccaga tggagttgca accgagtcaa ccatgcgtgt 71160
gtaaatgtaa acgtcatcgc ctctgatagc accataagtg ttacctagtg tatctgctag 71220
gtgtaagtta attggcgtca ttacagggcc aaccagcttg cctaataggt cacgtaggtg 71280
ttcacgatga gtagtactct ctgtcttgtt accaaggtta ttcatttggt caaacgtttg 71340
aaccaagttg tcttggctca ccttctgagt attctctcta gcgaagtcac gggtattgaa 71400
tcgtgcagcc gaagcagaga ataagatatc gtctgctgaa agctctgcat caattacttg 71460
aaccgcagtc attactttct caagagcatt gcctttcttc gcatcagtga taccgaacat 71520
ctctaggatt gcacctttaa cccaagcata agcagacttc actttgtttt cgtaagtgat 71580
ctgtgtttga gccaaagcat tctgcatctg ttttgatgtc agagtccaag cgatcatctc 71640
gtcgatgttg tcgatagagt tagccttgaa tgtatcgcgg tgattcttag ccagttcctt 71700
gcggatgtta gccaaatcat tcttagcttt gttcacttca ggattcttag aatctttcat 71760
tcgctcaact agtgcagcgt gaaccaattc gtgagcaata gtttcatcac ttaggccatt 71820
cactgcgtac tcgtcacctg caataagaat caacttctct ttagcaacat gtaggccacg 71880
gctattaacc attgaaccag agtacttcga gtgtttatct gcatcgaaca attctactgt 71940
gtagtcacgt ggtagtaggt cacctagtga tagtagagtc tgacgaatag cacggtcttt 72000
agaatgtttc ttcaagaacg ctacagtctc aacacttgtt tttgaaccag aagctaagaa 72060
ctctttgata ccttcatcca ctggataaat gttctcttgg ttctcagtcg atactgattt 72120
tggttcaggt ttagcagcat acgtatcgtt caaacgagac actacaaact caagttgagt 72180
tggttcaaat acttcaagag cattcttcac tgtaccggca cggtattcag ttttgaagtt 72240
agccaaagca ttagcaaatg cagtagcacg ctcaccacct tgttcggtat aacgatgcat 72300
tagtcgatca acaacaatgt ccatacgacc tttgttacca gtcataccac ggtggatctt 72360
accgtaagct tcttcataag aaatagtctt tggttcagca cctttattac cagttacgta 72420
gccaccattc tccatgtagt actgagacat gtatgtgata cgtggtaata gcgagttacg 72480
gccttcctcg gtatcagtta ctgtatcgtt gattgaagca aggaagttct cccaaccttt 72540
atcagcagca gttaagttag acgctttaac acgatcaata ccgtatcgag tagcttcttc 72600
gttcactacc attaggctac gggcagtttg ttcagagttt agtacgttag taccattctc 72660
ttcatcgaac ttacgtgcag cttctagagc gcgttgtagc gattcattag cttgagccac 72720
gatagagtaa tcgttcataa cttgattaaa gttacggttg attgcttcca tatctgttag 72780
gccattagcc gtaccagtaa agatcgcatc gtgtacgttt agtacgcctg cattttctag 72840
agtaagtagc attgtcgcag catctgtacc ttgtacagaa agtactgttg gacttacacc 72900
accgtctacc ggagtagtta ctcgaccaaa tgcgttacga gtcttagcac catcaattgc 72960
atacttacct ttacgcttag cctctgtacg gaactgagaa gtctccattg ggatattctc 73020
tgtcttacct acaaagatag cttcgttcaa gttaccagat tgtgctgaga aatatgagtg 73080
gattactggc tgtgcctgtg ccaactcttg ttcaacttca cgcatcatac gcagaggtag 73140
tgtctcacgt ttaccagaga tcagaccttt ctcgatagct tcttgagttt tcttctcgat 73200
aagaatatcg cgttgctgtg catacagatc gaatacgact ttcatcatat cgttcacttt 73260
agtacgacga gccataaact cagaatagtt atcttggata gcgttaaata ccgcttcgcc 73320
atagacttct gaaccaaaat taacaatatc gttttcacga cggtatggta gtttgaaatc 73380
caatgggttc ttaactagct cattagtttg agacttagtt agctgaagca ttgaaccaag 73440
attacgaaga tcttgttctg tacgcttgga gtcgccaacg tagtcataca ctttgtctag 73500
tagcatgtgg cccatagctt tagtgatcgc tttctcacct gaaccataga tagctgtcat 73560
cagtgggttc ttagtcaggt tacgacctgc tttagttacc acttcacgat cagtaccact 73620
tagtgcggca gttagctcac ccattactgt gcttagtaga gctgcatcac gttcacttag 73680
gttgtcgata cgtttaacta catcacgagc aattgcttgg tagttatcat cgttcagtgg 73740
atcagaagac ttccaatcac caaactgttg agtatcgcca tctaggttaa tacctacacg 73800
tttcagacgc tcacctacat tgtcatcaga tgcaccgata gcaaactgta gtgaagcaat 73860
agctgggccg ttagtgatac cgtcaccctc gatagagtaa tcagtttcaa actcagtacg 73920
accttggcgt agagccagca tgtactcaga gtaagcaaca agtgcatcca ttgagtgagc 73980
agcttcacct gctttatcaa ctgtagccag aatcttatcc atatcgaatg gcttgtcttc 74040
tagcatcttg attgtctctt ggataccttc attgaatact ggtgtttgaa ccaattcttc 74100
taattcgtca cgagacattt gatcagtctg tttgtcgatg cttacgccaa agccttgagc 74160
aataccaaca agaaacagat cagtattatc catggaaata gactgacgct gatcaccaac 74220
atatagaaga tgacggtgta gcttatcgcc ttgagggtta accgtattgg agacgatacc 74280
gacacgttgg ttcttccact gttcccagtt gaagtagaac ggttgttctt cgttttccaa 74340
gctttcatgg aactctctcc agtatttaac cgagttcatg atttgaccat tcttaccttc 74400
tacaccagca cgttgatgtt gctgaactgt agcagggtct ttaaggccca tcattcgacc 74460
gatttgttca tctgtcattt gagagaatag tttctgttgg ttcttcttaa caaagtgctc 74520
acgttgtgag tgcttcaata agatatcctg tactttctta gccactgact gacgagtacc 74580
tttctgacga gttactgtct tagttggttt ctcagtagtt gggccacggc ggaatgtttc 74640
agttttgaac agacgaccaa gtacatcatt tgaaccagaa gtatcttcac cgaatgaacg 74700
agcattatca gtgtctacca ttgagtagaa aaagatctca cgattctcat caatatcttt 74760
agctgtgttt gtgtcatcca aaccatcaac aatacgtgcc agttcttttt ggttcacttt 74820
gttaagttgg attaggccat taccacgaag catatctaga ccaaatagac ctaaagatgt 74880
ttctaagtta gtttgggcat tgctacctgc atccgggcca gctttgatac caagagttct 74940
aacaatagct gaaccaagtt catcaatgat gtttgactgg tgagtaccga cgttctgtag 75000
tagatcacgt agctcaggag aaacatactg ttcttcttga cgaccaacaa tgttgttgat 75060
gtcttcttgg ctattaaacg tagtctctgg agctttatga gccatccagt tatacatagc 75120
caaagcaaca gatgctttca cgttctcatc aaatgaacca tcgtcattac gtaggtaaag 75180
catgtaatca gccggtgcat aatcacctgc cgcagcttca tcaaactcag cactaaccgc 75240
tttagcgaat tcagtaaatg aaccaattgc acgagactgt tcttcagtta acgtgtcttg 75300
gtctaaatac tcaactgctt tgttcacatc agctgatagt gtttcaactg cgtttggttc 75360
aacttgaagt gggctattag ttagaccttc ttcagcaaca gtagatacga acttctcacg 75420
aaccacattg gtttcagcaa atgctttatc agcttgctca ggtgaacgtt cttctgtagt 75480
tggttcaaca gtacgagtag ttaagcgttc tgccaactta ggagttggtt cagttacttc 75540
agcttgctcg acagtttcac taataggttc cacatcagca gtagtagtaa catcgctagt 75600
aggagcttca tctacagtaa cctctggttc aatagtagtt tcagtttcaa cagcagccgg 75660
agtctctact tctgtttcca caggagcagc cacgctgtca gtagtatcaa cagcaggagt 75720
ttccacagtt ccattttgta cctcacgtaa accatttagc atagtttgta gtgcagtagc 75780
cgcttctgct tcttgttcaa tctggttcac tagagaaggt gcaatacctt ctgtcttcag 75840
atcaatagaa cgcaatagtt gagcacgttc agcatcatca gtcgtattta gagcctgacg 75900
agccacagca gctttgtttt ggcgttgggc tacaaagtta tcaagatctg taacataacg 75960
ctgtacagca gcctcattgc caccttcgat agcattagaa atgatttcct gatactcgtt 76020
caaaccacga taacccggac taccttcaaa cacgtcttta gatacttcac cgatagattt 76080
attcagacgg ttaaagtcac gtgcagcagt tactacagta cgctgttctt cagttaagtt 76140
agttgaacca agtagagctt cagcgttttc atcgcttaga gattctgggt cgattaggat 76200
agatccaata actcgctcag tagctccagt atcgcctgcc accacttgtt gcgttgcttc 76260
ttcgatgttg tttgtttcaa gttgagcctt ttgtagttga gacatgatat tgctgtaacg 76320
acgaccctca gtacgcaatg cgtcagcacg cttctggaat tgagtcgctt gctcaccctc 76380
ggcagcatta gccgcttctt caagtgtagc aatctgttca gcatagtcag tccatacttt 76440
tacagcagct tcatagttct gtactttctc ttcttgggtt acaccttcaa cattgttgcg 76500
agatgcaata ccacgcagag ttccaccaat tgagaagttt ggatcattga catcagttgt 76560
aacgctctct gttccatttt cttcaatatc ctttttagtt gtacggttct gtttagccgc 76620
tttagcttta tcagttactg aaccagtagc ctcaatgatt gaagcagcag tgttcgcagc 76680
aacgccagaa gcaccacctg atactgcggc agcaaccgca ccagtagcag cagcttcacc 76740
aacaccttca gttaggtctt gggtttcatc taccgtttgt tgtttagcaa tgttagtagc 76800
tacttgtgaa ccaccagatg cacctgtctc ttctaatgct tctttagtag caccttgagc 76860
tgtagcagct aggttactta gtgtcttacc gacacgtgaa gcagggttga acaagttagc 76920
agagaattca ccaccaccag ttagacgaga tgatacgcca gcaaatagtg ccgagtaacc 76980
aaatgccaag ttaccagcag catcttgtag acgagctttc gcttcttcat ggcttaggcc 77040
atctgcaata aggttacgat actcaggaga attttgaacc aattcttcct cagtcatctt 77100
ctcgatttca gtaagtactt gtgcttgtga accaagacct tcttgtactg cagtagttgt 77160
tacacccaaa gcagtagcac gtttcttcag ttctttttga cctgcttctg tagcagccca 77220
cttagcaact gactctgcac cttgctcagc aatttctcgt ggcacagcca aagctttacc 77280
gataccacct gttaggaaga tatcagccac gttctcaaat gctaagtcga tagccaaaga 77340
tggacgactt gcgtatgcag caacagtgtt aaagaattca ccagcaactt ctttagcttt 77400
ctcagtacca gataactctc gatcaccgat ttcagcttta cgttcttcgc ggtatgcttc 77460
cagttcttct tggttagcaa agcgtcgagc ctgaattggt tcagactggg tattattgaa 77520
atcgtcaact agagtctctg catctttaag agcagtaaga atagtattac gttcttcttg 77580
gcgttgttga accagatctt cacgagtctg agtttcacga ccttgctctg ccagtgcagc 77640
ttgtgcacgt tgaaagtaag ggttctctag aacgtcatct acttctgcgt caggtaatgc 77700
agcacctaat gctgattgtc ctaaacgaac tgcaccacga cctgcggcaa tagcagcatc 77760
tgaaccaaat tcagttaatg aacgagtacg atcatctcga cgtaaacggt tctcagcagt 77820
attcgcataa tcacgaatct ccttgagagc agccatagtc ggatctaatt ccaattcaat 77880
ttgattagaa cgttctttaa cttgttgttg ttttgaacca cgtttctgag aaagttcagc 77940
ctgtttctga ctagacttac tattgcccga agccaatgca cggaactgtg ctagttggtc 78000
ttcagtagtg gtatttggtt caaatgtaga agccaagttc tgacgacgtt cttccaaacg 78060
ttgttgaact agttggtctt gcgcagattc ttgttcagga gtgaactgac cttcttcagg 78120
taagtcgatt ggattttggc gttcttcagc cataagataa atctccttga attgatccgt 78180
acattttcga tgtagtgtaa tacgttatca aggagaaata caggaaatgt gaaggggaac 78240
acagttcccc ctctatttag ttaatgtcac ggatggtttt acgcatttct tctactttag 78300
aaggaacatt gtaagtacgc gctcgtaatg cagcaggaac atcccaagct ttaccttgac 78360
ggatttgacg agtcattgct gcctcattct ttttgatttg gctctgtaag tcagccatac 78420
cagcatccat cacgtttaga gcaaggtttg ccttacgtac tgcatcttca tcagtgatta 78480
gattgtagta ctcacgggta gcatctcgga tatttgattc agagagccag cctgtcttat 78540
tacgatcagc tcggcctaag atgtaagcca tctcagcacc agttggttca cgatcaccta 78600
gttcctgttt agcaacacct aagtaatcag caaagacttc tttacccgaa tcaagtgccc 78660
actcaagtcg aggtgcataa cgcttagata ctgtctcaag agaagtctct gtatcgttac 78720
gataattgaa tacttctggt cgagcaccaa gtgtttgagc cgcttcttct tgaatagcct 78780
ccatctcacc ttcgaactgt ttaagagcct gttgaccata acgttcttgg gtttcgtatg 78840
tttgacgttg ttcaggtgac cacgagttgt aatactcttc atggaacttc agagcttcag 78900
tacgaacctt gttagcttct tcagtagaca agtcagtacg tttagctaac tgtgtagtca 78960
ggaagtcacc ataagcatta gcatcaccct caaagcgttt aagcgtctct agagtacgat 79020
agcttgaact tagcaagtct ttctgttgct gaatacctgc tgccgtatta gttgtcttag 79080
gcggcaccgt ataattacca atatgttcta ctcggcctgt attcgggtct tcacgtaaga 79140
tctgaccatt ctcatactta aacccatatt ggttagcctg ttgttgttgc aactggagtt 79200
tacgatcatt caaatcgtta ttgatctctt gctgctccaa cacattctga aacttctgtt 79260
gctccagatt acgatcaatc aaagtatctt ggcgtgaacc aaaaacctga ttaattgctg 79320
tctggtctac ttggttaccg aactgactag agtccacacc acttaaagct tcttgtgaag 79380
tagcgcggtt caactgatca ataatattgg cagtgttacg ctctttctgt gcttcatagt 79440
tagtttggtt cacattggtc tgttcttggg ccaaggaacg taagtcatta attgactgag 79500
agatacgatc agcacctgca tcgaaagcac ccaaggcacc acctgcatca ggtacttgaa 79560
cgttatccca tcggattggt cgagccatgg gttactcctt ataaacgact tttcttctgg 79620
ttgttacgta cttcagcata cgcagcttct tccgcagtac ggcgatcacg ttgatactcg 79680
aactcttcac gttgtagttc ccaagcttca cgctgtagac ctaattggtc tttagcaata 79740
cctagttgct ggaagccgag ccaagagtta gcaagtgtac caacggcacc gatagttgga 79800
gccaaagcac cagtagagaa attaccctgc tcatcagtac caccgaacag agtgttaaat 79860
gtactcatag ggttagtacc ttgagtgttt gaaccaattc cacctgtatc gaaactgtaa 79920
cctgagctag agccgacacc gtagttattg ctgtagttat tgtccattgc taggttaaag 79980
ccttgcatag cgtttgaacc taaaccgtaa tgatttggca tatggagatc tcctatactt 80040
taatgcccat gtgggtagct actccctcaa gggaaatcat gttgtctacg tagttttcaa 80100
tatgtgcttt atcaattgga gccaagttgc ccaagtgaat acgagtagta tagtactgtt 80160
ctgctgtctc atttggaaga gccaagccag accttgaatc cgggatacct aacggatcaa 80220
tcagaaatgc agtacttgtc tcagcataga actggttcat ttcttcttcg tactcattca 80280
tctctcgttg taaagcatcg tagtcatcca tgatatcact catttcagta tcaatgtaag 80340
cgttaacgcc tgtttcgata ccagactgta atgcacttaa cgtttgggcc agacctgtct 80400
ggtcaccacc aactgaacca agagcgatcg aagcgaagat agcaaacaca acaccagcag 80460
ctacaccaag ctcatcgacc aaatagttga taccatattt gataccaacc gtaatgaccc 80520
ctagagcgat ggcacctacc acagccgcag tcaaacctgt accaaggcca agccatgcac 80580
ccatagaggc agaggcagta ccaaaggtat aaatggtcag tacaatacca acaaccgcta 80640
caatggctga gaacaagcct gtttcgtacc atttaagctt ctgtttcaca taacagttga 80700
agttgatacg catagcttcg ttcactaagt cagtacggtc tacaacacgc atattttcaa 80760
ctagacgaat gtccaaaggg ataagcatac cgtctgagaa gtcatcttca ttctgtgccg 80820
cgctaactac gttagctaat gaggtatcta ctgtatgtga tcggtaaaca tagttaatgt 80880
ggttcaaacc acgtacttca acttcatcat atgaattggc agtaatctgc ttacgtacaa 80940
cgaaagaact atcttcataa ccgtagtact gaccagtaac ttgaggacga acaatgatct 81000
cagtgttcac atcatctggt tcagcatctg gttcaattac accagttagc aaagtagatt 81060
taatgtagag ccaacctagc tcaacatcat atgaagtacc tgcctcggaa atacgtaagc 81120
tgttaatcgg tggtggagca gtcagtttga aatcttcatc tctgtcattc cactgatcat 81180
atgcttcttt gtcataggta gagttattct ctttatcttt ccagaagtcg tacaggtagc 81240
tttgtgcagc tttaatctgt gtatttggag agatgcccat taatacgtaa gcatgatcaa 81300
tatcaccaat atccgggtta gcattaaccg cactacctag ttcagagata ctcagcttta 81360
actgtcttag taaacgtcga gaagtgttat agcgttctga accatcatct actgtaagat 81420
cagcattgtt ataccgaaga ggaacaacag gaaagtaatt agagtcaaac tcttcgtaag 81480
gttccagtac ttcataggtg ccctcgtcgg gagaatagaa ccagtaataa ataactgaaa 81540
gatctacttc ctcgtttgca tcgtatttgt agtacccaac gtggtaatac ttagcatctt 81600
ctaagattga accagaatag gtatatgtct cagtgaactc atttaagaat tcatctgtat 81660
aggtaagttc aatttggttc gtactaacga tctttgcgta agagaatact gtttcgcctt 81720
gagcacctga agggggatta cttactacta atgtagtttg gttcaatcct cgtgtaccta 81780
ataagtactc aagagcaaag tagtatccat ctgctgtact gtagattgaa aagtctacaa 81840
gcacatcctc tccgatttct tcagagataa cattctgaac ggtagtaggt gaagcacctt 81900
cattgttcat tgaaccagaa ggcactccat aatagtactc ttcagatccg taccggtaat 81960
atctcttcag gttggttccc aatccactaa tggtattggc aatcaaatca tcggtgatac 82020
tacgttcagc acggatagaa gtaagtactg attgttgaac caaattgggt acttcctcaa 82080
ctagcggagt cgagaaggac tccacataaa ttttagtctt tgaaaataat cccattggtt 82140
caatccttat gcttgtacag ttacgccaac accttcacga actttgttca agacatcttt 82200
gatctgtgta cctgttacgg tagcatccag agtttcgttc tcgtttagag actcgataga 82260
tgtacgggtg ttgaatgagt ccataaatag cttagcagct ttctgctcag catcacggat 82320
gtaaccttca gcttggttct catagagagc catttgctta ccgatgatac cacctgccgc 82380
agcaccaagt actgtgtctt tggtttggta ttcttcagta acacgcttct gtgtaagtac 82440
agcagtttct gcatcaagct tagaaccttg tttaacaatg ttagcttgct ctgcttgtag 82500
gttagcttct tgctgaccag ctagagatgc ttgtgcttct gtctgtagga tttgtgcatc 82560
tttaacttct agatctttat ctaggttaag agcttgctta tccagaatca gttcttgctt 82620
aacagcattc tgcagttctt gatccagtag caattgacgc ttcgtttcag aggttgtttg 82680
ctcattaaca aaggcaatat cggcagttag tttatcaatc tgcttttgaa ccaattctac 82740
attcttctcg gcagcaagaa tctgcttgtc caagagtcgg atgttttggt tcgtaatctg 82800
gtgttgaaga aggtagttaa cagagttagc taaagtacta tctaaagcag caatgtatga 82860
cttggtatac tgatcaccag taatacgtcc ggtcttatgt tcttccgcca gatgagactt 82920
aactgcacgc atcaattcgt caaaggcacc tgaaccagtt ctctcaccag aagtaagtga 82980
gtcaatggtt agtacctctg gatcaaagga tgtaatcgag gtcatctttt ttctcctata 83040
aaaaaggctc cacttattgt gaagcccttt ggttcaaaag tctaccggcc ttagtcgata 83100
gcttgacgtt tagcttggtc agcagctagt gcatctaact cttcttgagt caacggatct 83160
aagatctcaa tgttgtactc tttagggaag aagttcttac gatacttacc acctttacca 83220
tctggaacgt ctttagtctg acggaacact ttgttcttca gtgtatcaat catcatttga 83280
gataggtgat actcgtggtc aaacagaacg tatttagtgt ggaagcctag aactgagtta 83340
ccaatcgaca tccactcacc ttgccattca cgcttgtgag ggttcataca ggtgatacga 83400
cagcgaacaa gtttaagttt gtctttacgt agttgattac gtttatccat cttagtcttg 83460
ttaggggatg ctttaacagt atttgcttca gcagcctttt catcgaattc tttaatcttt 83520
tctttgagtt tctctaaaga gattgaagga tggaatttaa tgcccttgtt ctcggcaatt 83580
tgtttaagac tgtctagttc tgtgaattct tcagacataa tatgtacctt ttagttttgt 83640
atccgttcct gatctaagca ggagggtcaa aggctcccct aaagagagga gccatattta 83700
ttacagttca gctactgtct tagctagagc aatacgctct ggacgtagga tcagtgtacc 83760
gtagtaccac tggattgaac tgaagccagt acgaccgaat gggtcgtggt gttgtgcaac 83820
accttcacca ggcatcttag tgataatctt gaacttgaat gacttaccag aagtttggaa 83880
gccgatagtt gtgaacgatt ctgaaccaat acatagcatt gggaagatat cgtatgaacc 83940
accagtctca cggtaaccac cgttagtagt tactgcttta ccagcaccag cccagtgagt 84000
catctcaggc acaactacga tacggaattg accgacttta ccgatctcac cgtgaatcat 84060
gttcacgcct tttttgtagt cgccagcgta tgcgtactgt tctacaggaa caaatgcttt 84120
gttgccaaat gcatcagtca ttttctcgat ttgcatttgt agttctgaac caacgtaaag 84180
aacacgacct gcaccgatag tagaggtatc agtcatacgt gaaccagtta ggatcttcgt 84240
agacttagga gtacggttgt catccaactg gattgacata cgttgtagat cttcgtaagt 84300
gattactgat ttaacgtcag catcttcacc agtcatttcg atgtcttgag ttgcgtcacc 84360
accgaagtag ataacaccag caccgtttag aaggtcaatt tgtagaacgt cttcagtgat 84420
ctcagatgca ccacgaacca attcacgaga aatgtgttgg tataggtcat ccattgtatc 84480
gaagtttaga gcttcatctg tgaactcatg gaagaaacca aggttctcta gattcgcttc 84540
tagcaccaaa cgtgagtaac caacacggtt cacacgacca ccagtttcag aaagaagtgg 84600
tagcttagcg ttgatagtac cgatatcttt gcttgaacca tataggttac catcagcaat 84660
agttgcaccg gcagcgtcga taccttggtc gttcacgtta cgatcatcta gcaatggaag 84720
catatggtat ttcttaattg tcttaccaaa gtgcttaggc attgctgtta catcagcaag 84780
tggttggaag tactgttctc gaacggcttc aatcagagcc attttgtggt aatagtcctt 84840
gcggatctgt tcaccagcag aagagttagt accgtcaccc cacttaatct ggttagtgtc 84900
atttagagcc atagtaatct cctagtaagt ctctattaaa ttaaagtttg atcttctcaa 84960
actcttcatc agacatattc caccaatcat gtgactcagg ttgtggctta cgtcctttgt 85020
tctgagaaga acttgcctta cggcgtttat cttgcagttg cgtttcatca gctttaggct 85080
tcggtttacg aaccactttc ttaggcttct gttgtggctc ctgtggttgg gagtcattga 85140
acgcaccttg agaaaacaat ttgttaccta ctacctgata tgcctctaca tctgaaaggc 85200
cagctaaacc acctaacatt cgttcacgct gcaactcagt ggaaatcttg tcataaatgc 85260
cattggcttt atgttcattc atgattgaca gcaattgggg tttgcttgca agttcacgct 85320
tactagactc atcccagtta gaaacgacat tcattgtgtc tgaaaatgta ggagtcgagc 85380
gaatgttctc gattacttca tccagttcca cctcattgtc gccaactgca tagttactag 85440
gtgtgtactc tttaccttcc tccagttcca taacgtcaat gccgttagtt tgaaccagtt 85500
gggcaatagc gtctttgtta ccgttcatta aatcaatagc gaatgataac ttggactcgt 85560
caagaagctc attgttggca agcatcttaa cgtacttacg ctgttctttg agatctttag 85620
tcttacgggc ataatccaaa cccatcgaca taagtctacg tgcttcagca acgtctttag 85680
gttgaaactc tgaaccatga tgcttcaaag gagacatcag ttcttcgtac aacttatcaa 85740
attcttcttt tgaaccatct tctgtttcgt cgtctgagtc ttcttcagtc tcatcgtcgt 85800
catcagattc ttgatcatca tcttctgaac cagtttcgtc atcttcgtcg tcactgtcat 85860
cttcatcaga ctctgaatca tcttcttcag aatttggttc aaattcgtct tgatctgtat 85920
cttctgtgat ttctagatcg tcgtcaagac cttctgaacc attttcttct tctggttcaa 85980
aatcatcttc actagaattg tctacagcat caagttcatt attcagttca ccgccttctt 86040
ctcgatcacg gatttcagaa tcaacagaag gctcactagg agcctccgcg aataactgat 86100
ctggatctgc gctcaagaaa tcatcgtctg acatagaata aagattgtta ttcgtagttg 86160
tcataggatt actcctcgtt tactaggtct agcttagttt gctctagttc ttcaagtgca 86220
tgtttggcat gttggccttg catgtgtact tggtggaaga acatttctag ctgagcaaca 86280
ccagtcatac ctagctctgc tgctttacgc tgacgctcgt caggagaagc aaatagtgca 86340
gtaagcgtct taggcttatc ggcaatatac ttcttgagga ttagcttttt aaaagcagaa 86400
ctgcgttcta gtttttctag tgcttcagca agttcaactg cttctttagc gtcttcaatg 86460
gatacttcga tttcttgtag atcagtcatg ttaattactc cgtgtccccg gcatcttgct 86520
cgggattatc ttgaggtgtg ttttggttca aagcatgttc gaatgcttta agtttcatgt 86580
taccttccgc ttgagcttgt tgtttctgta ggtcacgtgc atggttcaca ccattctctt 86640
ctgaaacata atccaagttc ttacgatcgg tatcagcttg tttgttacca gtttctgcca 86700
agatcttctg gatctctgct tgcgtcttct ggatttccat ttgctgagcc tgaatgtcaa 86760
gttgtagctt ctgcatctcc atttgttgtt gtggatctgg ttcagcttca tattgacgaa 86820
ttctctctgc tagttctggc attttacgta aatcagcaat ttctgcaaga attgttttat 86880
acatgcctaa accgaagctt tgaccggttg tttgaagcat aaatgacaat tcctgagctt 86940
tttgctcatc tgcctgtgcc gtactgattg ttaatcttag gtcaaattga cccattaagt 87000
catcacgtct tacagtaaca aacttattat ttgtgatacg gatcacttct tcttcgctta 87060
ggaacgtttg gttcatagca ataatctgac gtgcagcttt cttcaagcca ttagctagac 87120
gacgaagaat acccaattca cgacgagctg ctgcatcaag tgcaccttga gcatgtgtag 87180
ctgtctcacc taaaccagca ccagtaatac cttgtgcgaa tgctttaaca ccagataatg 87240
actcagcttc ttgctgttgc attgtaatca tgttgtatgc agacattggt acttctgggt 87300
attggtgcat atagattgag ttctgtgcat caccaacatc gttatattcg tagtctagac 87360
cagcatcaaa acgacgacgg ttcattgggt ctagggcacc tttacgaata cctgtttgac 87420
cgttagcact acgtgccatt aagtcgatca taccacgagt tactgcgcca attactttct 87480
ggttatcagc gataagttca ccatctggtt caccataaac tgaaccatct acaggaagat 87540
agggaactgt cacaaatgga attttaccat gaggatatgg gttctcttcc agacggatga 87600
tagttgaacc aacccaagta caaacgattg ggcgagtttc acctgaacca tctacgtccc 87660
agtaacccca gtattcataa gctactaatt tctgacgagg tttatccgag aacataaatc 87720
cattttcagc ccagttactt gcatggtcac catcagataa cggtgtgttt gaaccagaaa 87780
cgattgaatc cagattctca taaatacctg ctttacgtag ctcagcaagt gaagtttcaa 87840
acgagtagat aacgaactgt gctttctcaa tatcaccatt acaagatgga tcaataatca 87900
cattctcttg gtcacatact tctagagtcg gttggttcat tgttactaca gtttcttctt 87960
cctcgacttc accagtctta tgaggatata 87990

Claims (10)

1.一株副溶血弧菌噬菌体，其特征在于，该噬菌体为副溶血弧菌噬菌体(VibrioParahemolyticus phage)RDP-VP-21010，保藏编号为CGMCC No.22481。
2.根据权利要求1所述的副溶血弧菌噬菌体，其特征在于，透过电镜观察，该副溶血弧菌噬菌体(Vibrio Parahemolyticus phage)RDP-VP-21010头部呈正六边形，头部直径约为56nm，尾部长度为103nm，为长尾科噬菌体。
3.根据权利要求1所述的副溶血弧菌噬菌体，其特征在于，该副溶血弧菌噬菌体(Vibrio Parahemolyticus phage)RDP-VP-21010的最适温度为37℃，效价最高达到1.7*10^12pfu/mL。
4.根据权利要求1所述的副溶血弧菌噬菌体，其特征在于，该副溶血弧菌噬菌体(Vibrio Parahemolyticus phage)RDP-VP-21010对水体中副溶血弧菌具有强裂解作用。
5.如权利要求1-4任一所述的副溶血弧菌噬菌体RDP-VP-21010在制备预防和或治疗副溶血弧菌引起的疾病的药物中的用途。
6.如权利要求1-4任一所述的副溶血弧菌噬菌体RDP-VP-21010在制备预防和或治疗副溶血弧菌引起的疾病的饲料中的用途。
7.一种用于防治副溶血弧菌的杀菌组合物，其特征在于，包括有效量的如权利要求1所述的副溶血弧菌噬菌体(Vibrio Parahemolyticus phage)RDP-VP-21010。
8.一种如权利要求1-4任一所述的副溶血弧菌噬菌体的应用，其特征在于，副溶血弧菌噬菌体(Vibrio Parahemolyticus phage)RDP-VP-21010用作为由副溶血弧菌引起的细菌感染提供潜在的治疗药物或保健品。
9.一种预防和或治疗副溶血弧菌引起的疾病的添加剂，其有效成分包括权利要求1所述的副溶血弧菌噬菌体(Vibrio Parahemolyticus phage)RDP-VP-21010。
10.一种水产养殖环境的清洁剂或消毒剂，其特征在于，包括如权利要求1所述的副溶血弧菌噬菌体(Vibrio Parahemolyticus phage)RDP-VP-21010。
CN202110894035.4A 2021-08-05 2021-08-05 一株裂解性、高效价副溶血弧菌噬菌体rdp-vp-21010及其应用 Pending CN113528463A (zh)

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Cited By (1)

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CN116732006A (zh) * 2023-08-09 2023-09-12 山东省农业科学院畜牧兽医研究所 副溶血弧菌噬菌体解聚酶及其应用

Citations (1)

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CN112195158A (zh) * 2020-10-09 2021-01-08 瑞科盟(青岛)生物工程有限公司 一株裂解性副溶血性弧菌噬菌体rdp-vp-19003及其应用

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
CN112195158A (zh) * 2020-10-09 2021-01-08 瑞科盟(青岛)生物工程有限公司 一株裂解性副溶血性弧菌噬菌体rdp-vp-19003及其应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116732006A (zh) * 2023-08-09 2023-09-12 山东省农业科学院畜牧兽医研究所 副溶血弧菌噬菌体解聚酶及其应用
CN116732006B (zh) * 2023-08-09 2023-11-17 山东省农业科学院畜牧兽医研究所 副溶血弧菌噬菌体解聚酶及其应用

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