KR102237011B1 - 병원성 살모넬라균 특이 신규 박테리오파지 kfs-sm 및 이를 포함하는 항균 조성물 - Google Patents

병원성 살모넬라균 특이 신규 박테리오파지 kfs-sm 및 이를 포함하는 항균 조성물 Download PDF

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KR102237011B1
KR102237011B1 KR1020200004179A KR20200004179A KR102237011B1 KR 102237011 B1 KR102237011 B1 KR 102237011B1 KR 1020200004179 A KR1020200004179 A KR 1020200004179A KR 20200004179 A KR20200004179 A KR 20200004179A KR 102237011 B1 KR102237011 B1 KR 102237011B1
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bacteriophage
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김수현
최인영
김연수
이해영
박미경
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Abstract

본 발명은 살모넬라균에 특이적인 사멸능을 갖는 신규 박테리오파지 KFS-SM, 상기 박테리오파지를 포함하는 살모넬라균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물에 관한 것이다. 본 발명의 박테리오파지 KFS-SM은 살모넬라균에 특이적 사멸능을 가지는 반면, 익균은 사멸시키지 않고 내산성 및 내열성이 우수하므로, 살모넬라에 의한 감염성 질병의 예방 또는 치료에 활용할 수 있을 뿐만 아니라, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제등 에도 광범위하게 이용될 수 있다.

Description

병원성 살모넬라균 특이 신규 박테리오파지 KFS-SM 및 이를 포함하는 항균 조성물{Novel Salmonella-specific bacteriophage and antibacterial composition comprising the same}
본 발명은 살모넬라균에 특이적인 사멸능을 갖는 신규 박테리오파지 KFS-SM, 상기 박테리오파지를 포함하는 사료 첨가용 조성물, 사료, 소독제 또는 세척제, 항생용 조성물 및 상기 박테리오파지를 개체에 투여하는 단계를 포함하는 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다.
살모넬라(Salmonella)는 장내세균과에 속하는 그람음성의 통성혐기성 세균이며 아포를 형성하지 않는 간균으로 사람·동물 및 조류의 장내뿐만 아니라 자연계에 널리 분포하는 병원성 미생물이다. 살모넬라는 인수공통 감염원을 포함하기 때문에 사람뿐만 아니라 돼지, 소, 닭 등을 비롯한 다양한 가축에 감염되어 질병을 발병시킬 수 있다. 사람에게 감염 시 식중독을 발병시켜 설사, 구토, 복통, 발열 심하게는 관절염 등의 만성질환을 유발하고 돼지, 소, 닭 등 가축에는 살모넬라증(Salmonellosis)을 유발하여 급성 및 만성 감염증을 발병시키는데 주로 오염된 사료, 물 등과 같은 요인에 의해 감염된다.
살모넬라균(Salmonella spp.)은 크게 2가지의 종(species)인 살모넬라 엔테리카(S. enterica) 또는 살모넬라 봉고리(S. bongori)로 분류되며, 그 중 대부분 속은 살모넬라 엔테리카로 생화학적 특성에 따라 6종류의 아종(subspecies)으로 분류된다. 살모넬라균은 생화학 시험을 통해 살모넬라 종을 동정한 후, Kauffmann-White의 항원표에 따라 혈청형(serovar)을 확인하여 최종적으로 균주명을 표기하며, 현재까지 약 2,659 여종의 혈청형이 보고되었다.
혈청형에 따른 살모넬라 감염 양상을 보면 과거에는 살모넬라 엔테리티디스(S. Enteritidis), 살모넬라 티피뮤리움(S. Typhimurium), 살모넬라 타이피(S. Typhi) 등 주요 혈청형에 편중되어 발병되었으나, 최근에는 새로운 혈청형을 가진 살모넬라균의 감염 빈도가 증가하고 있어 다양화 추세를 보이고 있다.
이 중 특히, 살모넬라 티피뮤리움, 살모넬라 엔테리티디스, 살모넬라 몬테비디오, 살모넬라 바레일리(S. Bareilly), 살모넬라 I 4,[5],12:i:-는 최근 질병관리본부에서 보고된 국내 주요 5가지 살모넬라 혈청형이다. 또한, 미국 질병통제예방센터(CDC)에 따르면 최근 살모넬라 파나마(S. Panama), 살모넬라 뉴포트(S. Newport), 살모넬라 센프텐버그(S. Seftenberg) 등에 의한 식중독 발병 보고도 증가하고 있는 실정이다. 실제로 미국에서 2017년 파파야, 2014년 치아씨드, 2012년 칸탈로프, 2005년 토마토로부터 살모넬라 뉴포트에 의한 감염이 확산되었고, 2016년 피스타치오로부터 살모넬라 센프텐버그, 2011년 cantaloupe로부터 살모넬라 파나마의 감염이 확산된 것으로 알려졌다.
현재 살모넬라 감염증을 예방 및 치료하기 위해 항생제를 주로 사용하고 있으나 살모넬라는 동물에 감염 시 세포 내에 침투하여 증식하고 감염하는 경우가 많아 항생제나 약제, 기타 생균제가 침투하여 작용하기는 어려우며, 최근 많은 분야에서 항생제 내성에 대한 문제가 발생하고 있어 이에 대한 원인을 항생제 오남용으로 규명하고 항생제를 충분히 사용하지 못하게 되었다. 이에 따라 산업동물의 배합 사료 내에 성장촉진제로 사용하는 항생제를 금지하기 시작하였고, 유럽연합에서는 2006년부터, 우리나라에서는 2011년 하반기부터 항생제 사용을 규제하기 시작하였다. 또한, 안전한 먹거리와 식품의 안전에 대한 소비자의 요구가 증대되고 있어 점차 항생제 사용이 줄어들고 있다. 세균을 제어하는 항생제의 사용이 줄어듦에 따라 세균성 질병의 발생이 증가하고 있어 항생제를 사용하지 않고 세균성 질병을 제어하는 방법에 대한 요구가 늘어나고 있다. 이러한 연구 중 하나가 자연계에 존재하는 세균을 특이적이고 효과적으로 사멸하는 박테리오파지를 이용하는 방법이다.
박테리오파지(bacteriophage)는 특정 세균을 감염시켜 감염된 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스를 의미한다. 박테리오파지는 항생제에 비해 숙주 특이성이 높아 특정 박테리아만을 사멸시킬 수 있으며 다른 박테리아에는 영향을 주지 않는다. 또한 박테리오파지는 박테리아에 감염(infection)한 후 박테리아 세포 내부에서 유전물질의 복제 및 조립 후, 자손 박테리오파지들이 박테리아 밖으로 나올 때 숙주인 박테리아의 세포벽을 파괴하는 방식으로 기존 합성 항생제의 기작과는 다르므로 기존 합성 항생제에 대한 감수성에 관계없이 항균 활성을 나타낼 수 있다. 특히 박테리오파지를 이용한 발명은 항생제 대비 개발시간이 짧고 개발비용이 적으며, 높은 특이성, 지속성 및 부작용 등의 측면에서 우위가 인정된다.
한편, 이러한 박테리오파지는 높은 특이성을 가지고 있고 균주에 따라 지속성 및 항균효과가 다르게 작용할 수 있기 때문에 여러 종류의 박테리오파지를 혼합하여 cocktail 처리하는 경우도 있어 다양한 종류의 박테리오파지가 필요하다. 이에, 본 발명자들은 넓은 범위의 특이성을 가지는 박테리오파지를 이용하여 단독처리만으로도 살모넬라균에 의한 감염증의 예방과 치료에 효과적인 박테리오파지의 분리 및 이의 응용에 관한 기술 개발에 주목하였다.
그 결과, 본 발명자들은 다양한 시료로부터 다양한 혈청형의 살모넬라 균주(strain)만을 특이적으로 사멸시킬 수 있는 파지를 분리 및 정제하고, 이를 KFS-SM으로 명명하였다. 또한, KFS-SM에 대한 특성 검토 실험을 통하여 높은 살모넬라(Salmonella spp.)에 대한 살균 및 정균효과와 온도, pH 안정성을 가지며 분류학상 시포비리대(Siphoviridae)에 속하는 것을 확인하였고, 유전자 분석을 통해 신규한 파지임을 확인함으로써 본 발명을 완성하였다.
본 발명의 목적은 살모넬라균에 특이적인 용균 활성을 갖는 신규 박테리오파지 KFS-SM를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 박테리오파지 KFS-SM을 포함하는, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 박테리오파지 KFS-SM을 인간을 제외한 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법을 제공하는 것이다.
따라서, 상기 목적을 달성하기 위하여, 본 발명은 살모넬라균에 특이적인 사멸능을 갖는, 신규 박테리오파지 KFS-SM을 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 항생용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 사료 첨가용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 소독제를 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 세척제를 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 인간을 제외한 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 KFS-SM은 살모넬라균을 특이적으로 사멸시키는 효과를 갖는다.
상기 박테리오파지 KFS-SM은 기존의 항생제 등의 화학물질에 비해 살모넬라균에 대한 특이성이 매우 높고, 살모넬라균에 감염하여 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있다.
또한, 상기 박테리오파지 KFS-SM은 내산성, 내열성이 우수하여 다양한 온도, pH 범위에서 살모넬라균에 의한 감염성 병의 예방 또는 치료용 물질로 활용될 수 있을 뿐만 아니라, 상기 박테리오파지 KFS-SM을 유효 성분으로 포함하는 항생제, 사료 첨가제, 사료, 소독제 또는 세척제 등으로 활용될 수 있는 효과를 갖는다.
또한, 상기 박테리오파지 KFS-SM 또는 이를 유효 성분으로 포함하는 항생제를 제공하여, 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주 범위의 문제점을 해결 할 수 있는 장점이 있다.
따라서, 본 발명의 박테리오파지 KFS-SM은 살모넬라균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.
도 1은 박테리오파지 KFS-SM의 살모넬라균에 대한 용균반 형성 활성을 검토한 것이다.
도 2는 전자 현미경을 통해 박테리오파지 KFS-SM의 형태학적 특성을 검토한 것이다.
도 3은 박테리오파지 KFS-SM의 열 안정성을 검토한 결과를 나타낸 것이다.
도 4는 박테리오파지 KFS-SM의 pH 안정성을 검토한 결과를 나타낸 것이다.
도 5은 박테리오파지 KFS-SM의 살모넬라균에 대한 살균 및 정균효과를 검토한 챌린지 어세이 결과를 나타낸 것이다.
도 6은 박테리오파지 KFS-SM의 흡착능 결과를 나타낸 것이다.
도 7은 박테리오파지 KFS-SM의 1단 증식 곡선(One-step growth curve) 분석 결과를 나타낸 것이다.
이하, 실시예를 통하여 본 발명을 보다 상세히 설명하고자 한다. 그러나, 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.
본 발명의 박테리오파지 KFS-SM은 축산물도매시장의 돼지 허파로부터 시료를 채취하여 분리한 박테리오파지로서, 박테리오파지 KFS-SM으로 명명하고 2019년 07월 11일에 생물자원센터에 기탁번호 KCTC 13880BP로 기탁하였다.
본 발명은 살모넬라균(Salmonella)에 특이적인 사멸능을 갖는, 기탁번호 KCTC 13880BP 박테리오파지 KFS-SM을 제공한다.
본 발명에서, 용어 "박테리오파지(bacteriophage)"는 특정 세균에 감염하여 당해 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스로, 단일 혹은 이중 사슬의 DNA 또는 RNA를 유전 물질로 포함하는 바이러스를 의미한다.
본 발명의 박테리오파지 KFS-SM은 살모넬라균에 대해 용균활성이 탁월하며, 상기 박테리오파지 KFS-SM은 각이 진 머리와 꼬리를 갖는 형태인 시포비리대(Siphoviridae)에 속함을 확인하였다. 또한, 상기 박테리오파지 KFS-SM는 열, pH에 대한 안정성이 우수한 특성을 가지고 있다.
상기와 같은 살모넬라균 특이적 용균 활성, 내산성, 내염기성 및 내열성은 본 발명의 박테리오파지 KFS-SM은 살모넬라균에 의한 감염성 질병의 예방 및 치료용 조성물 및 상기 박테리오파지 KFS-SM을 유효성분으로 포함하는 다양한 제품에 적용함에 있어, 다양한 온도 및 pH 범위의 적용을 가능하게 한다.
본 발명의 박테리오파지 KFS-SM은 pH 3.0 내지 pH 11.0의 범위 내에서 용균 활성이 유지되나, 이에 제한되는 것은 아니다.
본 발명의 박테리오파지 KFS-SM은 -10˚C 내지 - 60˚C의 범위 내에서 용균 활성이 유지되나, 이에 제한되는 것은 아니다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 항생용 조성물을 제공한다.
본 발명에서, 용어 "항생용 조성물"은 약제 형태로 동물에게 제공되어 균을 사멸시킬 수 있는 제제를 의미하며, 방부제, 살균제, 항생제 및 항균제를 총칭하는 것이다. 또한, 항생용 약약용, 식품용, 화장용 등 다양한 용도를 포함하나 이에 제한되지는 않는다.
본 발명의 박테리오파지 KFS-SM은 기존 항생제에 비하여 살모넬라균에 대한 감염성 질병에 대한 특이성이 높으므로, 유익균은 죽이지 않고, 특정 병원균만 사멸시킬 수 있고, 약물 내성 내지 저항성을 유도하지 않아, 기존의 항생물질에 비하여 제품수명(life cycling)이 긴 신규 항생제로서 이용될 수 있다.
본 발명의 박테리오파지는 살모넬라균(Salmonella) 에 속하는 균주에 대하여 항균 활성을 포함하는 것을 특징으로 한다. 보다 상세하게는, 살모넬라 속(Salmonella spp.) 에 속하는 균주에 대하여 항균 활성을 가지는 것이며, 이에 제한 되는 것은 아니나, 본 발명의 일 실시예에서는 살모넬라 균주 중, 살모넬라 듀블린(S. Dublin), 엔테리티디스(S. enteritidis), 살모넬라 몬테비데오(S. Montevideo), 살모넬라 뉴포트(S. Newport), 살모넬라 파나마(S. Panama), 살모넬라 살라메(S. Salamae), 살모넬라 센프텐버그(S. Senftenberg), 살모넬라 타이피(S. Typhi) 및 살모넬라 타이피뮤리움(S. Typhimurium)으로 이루어진 군에서 선택된 1이상에 대한 용균 활성이 있음을 확인하였다. 상기 살모넬라 균은 병원성을 가지는 것으로 감염 시, 식중독과 같은 질병을 유발하는 균주인 것을 특징으로 한다. 즉, 본 발명의 박테리오파지는 살모넬라 균, 특히 병원성 살모넬라 균에 대한 항균 활성을 가지는 것을 특징으로 한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 사료 첨가용 조성물을 제공한다.
축산업이나 수산업에서 사용되는 사료 첨가용 항생제는 질병의 예방 목적으로 사용되고 있는데, 예방 목적의 항생제 투여는 내성균 발생 가능성을 높이고 가축에 잔류하는 항생제가 사람에게 전달될 수 있어서 문제가 되었으며, 항생제가 육류를 통해 인체에 흡수되면 항생제 내성을 유발해 질병의 확산을 부를 수도 있다. 또한, 사료에 섞여 먹이는 항생제의 종류가 많고 이는 다제 내성균 발생 확률이 높아지는 문제점이 있어 자연 친화적이면서도 기존 항생제의 사용에서 발생한 문제를 해결할 새로운 사료 첨가용 항생물질로서 본 발명의 상기 박테리오파지 KFS-SM을 이용할 수 있다.
또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는 사료를 제공할 수 있으며, 본 발명의 사료는 박테리오파지를 사료 첨가제 형태로 따로 제조하여 사료에 혼합시키거나, 사료 제조 시 직접 첨가하여 제조할 수 있다. 본 발명의 사료 내 박테리오파지는 액상 또는 건조상태일 수 있으며, 바람직하게는 건조된 분말 형태이다. 건조방법은 통풍건조, 자연건조, 분무건조 및 동결건조가 가능하지만, 이에 제한되는 것은 아니다. 본 발명의 박테리오파지는 분말형태로 사료 중량의 0.05 내지 10 중량 %, 바람직하게는 0.1 내지 2 중량 %의 성분비로 혼합될 수 있지만, 이에 제한되는 것은 아니다. 또한, 상기 사료는 본 발명의 박테리오파지 외에 사료의 보존성을 높일 수 있는 통상의 첨가제들을 추가로 포함할 수 있다.
본 발명의 사료 첨가용 조성물에는 비병원성의 다른 미생물이 추가로 첨가될 수 있다. 첨가될 수 있는 미생물로는 단백질 분해 효소, 지질 분해효소 및 당 전환 효소를 생산할 수 있는 바실러스 서브틸리스(Bacillus subtilis)와 같은 고초균, 소의 위와 같은 혐기적 조건에서 생리적 활성 및 유기물 분해능이 있는 락토바실러스 균주(Lactobacillus spp.), 가축의 체중을 증가시키며 우유의 산유량을 늘리고 사료의 소화 흡수율을 높이는 효과를 보여주는 아스퍼질러스 오리자에(Aspergillus oryzae)와 같은 사상균 및 사카로미세스 세레비지에 (Saccharomyces cerevisiae)와 같은 효모로 구성된 군으로부터 선택될 수 있다.
본 발명의 박테리오파지 KFS-SM을 포함하는 사료에는 식물성으로는 곡물류, 근과류, 식품가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류, 곡물부산물류 등이 있으며, 동물성으로는 단백질류, 무기물류, 유지류, 광물성류, 유지류, 단세포 단백질, 동물성 플랑크톤류, 남은 음식물 등이 있으며 이에 제한되는 것은 아니다.
본 발명의 사료 첨가용 조성물에는 품질 저하를 방지하기 위하여 첨가하는 결착제, 유화제, 보존제 등이 포함될 수 있고, 효용 증대를 위하여 사료에 첨가하는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 비단백태질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등이 포함될 수 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있다.
또한, 본 발명은 박테리오파지 KFS-SM을 포함하는, 음용수 첨가제를 제공한다.
본 발명의 음용수 첨가제는 상기 박테리오 파지 KFS-SM 또는 이를 포함하는 조성물을 음용수 첨가제 형태로 따로 제조하여 사료 또는 음용수에 혼합하는 방식으로 사용되거나, 음용수 제조 시 직접 첨가하는 방식으로 사용할 수 있다. 상기와 같이 음용수에 혼합하여 공급함으로써 지속적으로 살모넬라균의 숫자를 감소시킬 수 있다.
본 발명에서 음용수는 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용되는 음용수를 사용할 수 있다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 소독제를 제공한다.
살모넬라균에 특이적 사멸능을 갖는 본 발명의 박테리오파지 KFS-SM을 포함하는 소독제는 병원감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 양식장 등의 건물, 축체, 음수, 깔짚, 난좌, 운반차량, 식기 등의 각종 생육 용품의 소독 등에 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 세척제를 제공한다.
본 발명의 박테리오파지 KFS-SM은 살모넬라균에 특이적 사멸능을 가지므로, 상기 살모넬라균에 노출되었거나 노출될 가능성이 있는 동물의 피부 표면 또는 신체 각 부위 등을 세척하는 용도로도 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 KFS-SM은 살모넬라균에 특이적인 사멸능을 가지므로, 살모넬라균에 의한 감염성 질병의 예방 또는 치료에 이용될 수 있다.
본 발명에서, 살모넬라균에 의한 감염성 질병은 상기 질병으로 인해 나타나는 증상, 예컨대 복통, 설사, 구토, 발열, 두통과 같은 일반적인 식중독 증상을 포함한다. 또한, 임신부, 영유아, 노인과 면역능력이 저하된 환자들의 경우 패혈증, 뇌염 및 수막염, 뇌 농양 등과 같은 중추신경계 증상을 포함한다.
본 발명의 용어 "개체"는 인간을 제외한 동물을 모두 포함할 수 있으며, 바람직하게는 포유류 또는 어류를 말하나, 이에 제한되는 것은 아니다.
본 발명의 용어 "예방" 이란 조성물의 투여로 질병을 억제시키거나 발병을 지연시키는 모든 행위를 의미한다.
본 발명의 용어 "치료"란 조성물의 투여로 상기 질병의 증세가 호전되거나 상기 질병의 억제 또는 경감 및 이롭게 변경되는 모든 행위를 의미한다.
본 발명의 상기 용어 "가축류"란 인간에 의하여 순화, 개량되어 사람과 함께 공동생활을 하는 유용한 동물을 지칭하는 개념으로, 예를 들어, 돼지, 소, 닭, 말, 오리 또는 개 등을 포함하나, 이에 제한되는 것은 아니다.
본 발명의 방법에서 상기 박테리오파지 KFS-SM 또는 조성물은 약학적 제제의 형태로 동물에게 투여되거나, 가축의 사료 또는 음용수에 혼합하여 이를 섭식시키는 방법을 통해 투여될 수 있다. 본 발명의 방법에서 상기 박테리오파지 KFS-SM 또는 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있다.
본 발명의 방법에 투여되는 상기 박테리오파지 KFS-SM의 적합한 총 1일 사용량은 올바른 의학적 판단범위 내에서 처치에 의해 결정될 수 있다는 것은 당업자에게 자명한 일이다. 특정 개체에 대한 구체적인 치료적 유효량은 달성하고자 하는 반응의 종류와 정도, 동물의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여시간, 투여 경로 및 조성물의 분배율, 치료기간, 구체적 조성물과 함께 사용되거나 동시 사용되는 약물을 비롯한 다양한 인자와 의약 분야에 잘 알려진 유사 인자에 따라 다르게 적용하는 것이 바람직하다.
본 발명의 약학적 조성물은 103-1010 PFU/ml의 박테리오파지를 포함하며, 바람직하게는 106-109 PFU/ml의 박테리오파지를 포함한다. 본 발명에 사용된 용어, "PFU (plaque forming unit)"는 박테리오파지가 세균 사멸 후 나타나는 용균반을 수치화한 단위이다.
본 발명의 약학적 조성물은 약학적으로 허용 가능한 담체를 추가로 포함할 수 있다.
본 발명에서 용어, "약학적으로 허용 가능한 담체"란 생물체를 자극하지 않고 투여 화합물의 생물학적 활성 및 특성을 저해하지 않는 담체 또는 희석제를 의미한다. 액상 용액으로 제제화되는 조성물에 있어서 허용되는 약제학적 담체로는, 멸균 및 생체에 적합한 것으로서, 식염수, 멸균수, 링거액, 완충 식염수, 알부민 주사용액, 덱스트로오스 용액, 말토 덱스트린 용액, 글리세롤, 에탄올 및 이들 성분 중 1 성분 이상을 혼합하여 사용할 수 있으며, 필요에 따라 항산화제, 완충액, 정균제 등 다른 통상의 첨가제를 첨가할 수 있다. 또한 희석제, 분산제, 계면활성제, 결합제 및 윤활제를 부가적으로 첨가하여 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립 또는 정제로 제제화할 수 있다.
본 발명의 약학적 조성물은 질환 부위에의 도포 또는 분무하는 방법으로 이용할 수 있으며, 그 밖에 비강 분무, 경구 투여 또는 비경구 투여를 통해 투여할 수도 있으며, 비경구 투여의 경우 정맥 내 투여, 복강 내 투여, 근육 내 투여, 피하 투여 또는 국부 투여를 이용하여 투여할 수도 있다.
본 발명의 약학적 조성물의 적합한 도포, 분무 및 투여량은 제제화 방법, 투여 방식, 대상이 되는 동물 및 환자의 연령, 체중, 성, 질병 증상의 정도, 음식, 투여시간, 투여 경로, 배설 속도 및 반응 감음성과 같은 요인들에 의해 다양하며, 보통으로 숙련된 의사나 수의사는 목적하는 치료에 효과적인 투여량을 용이하게 결정 및 처방할 수 있다.
본 발명의 약학적 조성물을 유효성분으로 포함하는 경구 투여용 제형으로는, 예를 들어 정제, 트로키제, 로렌지, 수용성 또는 유성현탁액, 조제분말 또는 과립, 에멀젼, 하드 또는 소프트 캡슐, 시럽 또는 엘릭시르제로 제제화할 수 있다. 정제 및 캡슐 등의 제형으로 제제화하기 위해, 락토오스, 사카로오스, 솔비톨, 만니톨, 전분, 아밀로펙틴, 셀룰로오스 또는 젤라틴과 같은 결합제, 디칼슘 포스페이트와 같은 부형제, 옥수수 전분 또는 고구마 전분과 같은 붕괴제, 스테아르산 마그네슘, 스테아르산 칼슘, 스테아릴푸마르산 나트륨 또는 폴리에틸렌글리콜 왁스와 같은 윤활유를 포함할 수 있으며, 캡슐 제형의 경우 상기 언급한 물질 외에도 지방유와 같은 액체 담체를 더 포함 할 수 있다.
본 발명의 약학적 조성물을 유효 성분으로 포함하는 비경구 투여용 제형으로는, 정맥주사 또는 근육 내 주사 등의 주사용 형태, 호흡기를 통하여 흡입이 가능하도록 하는 에어로졸제 등 스프레이용으로 제제화할 수 있다. 주사용 제형으로 제제화하기 위해서는 본 발명의 조성물을 안정제 또는 완충제와 함께 물에서 혼합하여 용액 또는 현탁액으로 제조하고, 이를 앰플 또는 바이알의 단위 투여용으로 제제화할 수 있다. 에어로졸제 등의 스프레이용으로 제형화하는 경우, 수분산된 농축물 또는 습윤 분말이 분산되도록 추진제 등이 첨가제와 함께 배합될 수 있다.
본 명세서에서 달리 정의되지 않은 용어들은 본 발명이 속하는 기술 분야에서 통상적으로 사용되는 의미를 갖는 것이다.
이하, 본 발명을 실시예, 제제예에 의해 상세히 설명한다. 단, 하기 실시예, 제제예는 본 발명을 예시하는 것일 뿐 본 발명의 내용이 하기 실시예, 제제예에 한정되는 것은 아니다.
<실시예 1> 신규한 박테리오파지의 분리 및 배양
<실시예 1-1> 살모넬라균( Salmonella )에 감염하는 박테리오파지의 스크리닝 및 단일 박테리오파지의 분리
살모넬라균(Salmonella)에 특이적인 박테리오파지를 스크리닝 및 분리하기 위해서 용균반 분석을 수행하였다. 대구광역시 소재 축산물도매시장으로부터 수득한 돼지의 허파 25 g을 시료로 준비하고, 이를 이용하여 탑-아가(top-agar), 0.4% 한천을 이용하여 고체 배지 위에 붙이는 소프트 아가 오버레이(soft agar overlay) 방법을 수행하였다.
구체적으로, TSB 배지 225 ml에 샘플 시료(액) 25 g와 살모넬라균(Salmonella spp.) 진탕 배양액 (OD640 = 0.353) 1%를 혼합하고 이를 37˚C에서 18시간 동안 배양한 다음, 상기 배양액을 8,000 rpm에서 10분간 원심 분리하여 그 상등액을 0.25 μm 필터로 여과하였다. 그 다음, TSA 평판 배지 상부에 TA 소프트 아가 (0.4%(w/v) 아가, 영양배지, NaCl, MgSO4, CaCl2) 4 ml와 살모넬라균(Salmonella) 진탕 배양액 (OD640 = 0.353) 200 μl을 넣은 혼합액을 부어 굳힌 후, 그 위에 상기 돼지 허파 시료액 10 μl를 적가하고 37˚C에서 18시간 동안 배양하여 용균반의 형성 여부를 확인하였다. 용균반 형성을 확인한 결과를 도 1에 나타내었다.
도 1에 나타낸 바와 같이, 용균반을 형성하는 박테리오파지를 확인하였고, 상기 박테리오파지는 살모넬라균에 특이적인 용균능을 갖는 박테리오파지임을 확인하였다.
또한, 상기 형성된 용균반으로부터 단일 박테리오파지를 분리하고자 하였다. 구체적으로, 상기 용균반을 900 μl의 SM 완충용액(50 mM Tris-HCl, 100mM NaCl, 10mM MgSO4)에 넣은 후, 실온에서 2시간 동안 방치하여 박테리오파지 용액을 수득하였다.
<실시예 1-2> 박테리오파지의 대량 배양 및 정제
상기 실시예 1.1에서 확인한 살모넬라균(Salmonella)에 특이적인 박테리오파지를 대량 배양하기 위하여, 살모넬라균을 이용하여 대량으로 배양하고 이로부터 박테리오파지를 정제하였다.
구체적으로, 살모넬라균을 진탕 배양하여 TA 배양액(nutrient broth, NaCl, MgSO4, CaCl2) 3 ml에 분주한 후, 2시간 동안 37˚C에서 배양시켰다. 상기 실시예 1.1에서 수득한 박테리오파지를 분주하여 2시간 배양시킨 후, 원심분리 후 필터링하여 박테리오파지만을 분리하였다. 동일한 방법으로 TA 배양액의 양을 증가(8, 20, 200, 800 ml의 순서)시켜가며 진행하였다. 그 다음 최종 농도가 10%(w/v)가 되도록 폴리에틸렌 글리콜(polyethylene glycol, PEG)과 1M NaCl을 가한 후 4˚C에서 16시간 동안 정치한 후, 4˚C에서 8,000 rpm으로 20분간 원심 분리하여 침전물을 수득하였다.
정제된 파지는 CsCl 밀도 구배 울트라원심분리(밀도 구배: 1.20, 1.30, 1.40, 1.45, 1.50, 1.70g/ml; 22,000rpm; 2시간; 4˚C)하고, SM 완충용액(50 mM Tris-HCl, 100mM NaCl, 10mM MgSO4)에서 투석한 후 사용 전까지 4˚C의 암소에 보관하였다.
이와 같이 분리된 박테리오파지를 “박테리오파지 KFS-SM (Bacteriophage KFS-SM)"로 명명하고, 2019년 07월 11일 생물자원센터(Korean Collection for Type Cultures, 한국)에 기탁하고, 기탁번호 KCTC 13880BP를 부여받았다.
<실시예 2> 박테리오파지 KFS-SM의 형태학적 특성
박테리오파지 KFS-SM의 형태학적 특성을 알아보기 위해, 투과전자현미경 관찰을 수행하였다. 박테리오파지 KFS-SM을 구리 그리드에 로드(load)한 후에, 2% 우라닐 아세테이트로 음성 염색하고 건조하였다. 전자현미경 촬영은 경북대학교 공동실험실습관에서 수행하였고, 박테리오파지 KFS-SM의 형태는 H-7100 (Hitachi)를 이용해 100 kV의 전압으로 관찰한 후, 그 결과를 도 2에 나타내었다.
도 2에 나타낸 바와 같이, 박테리오파지 KFS-SM에서 각이 진 머리(icosahedral head)와 꼬리(tail)가 관찰되었으므로 시포비리대(Siphoviridae)에 속하는 것으로 추정하였다.
<실시예 3> 박테리오파지 KFS-SM의 열 안정성 조사
박테리오파지 KFS-SM의 열 안정성을 확인하기 위하여, 다양한 온도 범위(-70˚C, -20˚C, -10˚C, 4˚C, 10˚C, 20˚C, 30˚C, 40˚C, 50˚C, 60˚C, 70˚C)에서 KFS-SM의 활성을 측정하였다.
구체적으로, 1.03 × 108 PFU/ml 농도의 박테리오파지 KFS-SM 용액 100 μl와 TSB 배지용액 900 μl를 혼합한 후, 각각의 온도(-70˚C, -20˚C, -10˚C, 4˚C, 10˚C, 20˚C, 30˚C, 40˚C, 50˚C, 60˚C, 70˚C)에서 1시간 동안 상온에서 정치하였다. 한 시간 반응 시킨 KFS-SM을 소프트 아가 오버레이 방법을 이용하여 37˚C에서 18시간 동안 배양한 후, 잔존하는 박테리오파지 KFS-SM의 활성을 측정하였다. 결과를 도 3에 나타내었다.
도 3에 나타낸 바와 같이, 박테리오파지 KFS-SM는 -10-60˚C에서는 안정하였으며, 70˚C부터는 약 4 log로 활성이 감소됨을 알 수 있었다.
따라서, 본 발명의 박테리오파지 KFS-SM은 내열성이 비교적 우수한 박테리오파지이므로, 사료 첨가제로 이용할 경우 박테리오파지의 제형 과정에서 발생하는 열에 대해 안정적으로 제형화할 수 있음을 확인하였다.
<실시예 4> 박테리오파지 KFS-SM의 pH 안정성 조사
박테리오파지 KFS-SM의 pH 안정성을 확인하기 위하여, 다양한 pH 범위(pH 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)의 TSB 배지 900 μl와 1.03 × 108 PFU/ml 농도의 박테리오파지 KFS-SM 100 μl를 혼합한 후, 1시간 동안 상온에서 정치하였다. 한 시간 반응 시킨 KFS-SM을 소프트 아가 오버레이 방법을 이용하여 37˚C에서 18시간 동안 배양한 후, 잔존하는 박테리오파지 KFS-SM의 활성을 측정하였다. 측정 결과를 도 4에 나타내었다.
도 4에 나타낸 바와 같이, pH 3.0 내지 pH 11.0의 범위에서는 박테리오파지 KFS-SM의 활성이 안정적으로 유지됨을 확인하였다.
따라서, 본 발명의 박테리오파지 KFS-SM은 pH 3.0-11.0의 범위에서 그 활성이 유지됨을 확인하였으므로 pH 안정성이 우수한 박테리오파지임을 확인하였다.
<실시예 5> 박테리오파지 KFS-SM의 챌린지 어세이 분석
살모넬라균을 TSB 100 ml에 접종하고 190 rpm으로 37˚C에서 3시간 동안 배양시켰다. 배양액의 흡광도 값이 640 nm에서 약 0.353에 도달하면 (108 CFU/ml), KFS-SM 현탁액 1 ml를 MOI 0.1, 1, 10, 100으로 맞추고 37˚C에서 110 rpm으로 12 시간 동안 배양하여 파지의 용균 활성을 확인하였다. 살모넬라균을 첨가하지 않은 KFS-SM 배양액을 대조군으로 사용하였다. 초기 균의 농도는 희석 후 6 Log CFU/mL 값으로 하여, 2 시간 간격으로 생균수 측정을 하였으며, 결과를 도 5에 나타내었다.
그 결과, MOI와 관계없이 0.1, 1, 10, 100 모두에서 8시간동안 용균활성이 지속되었다. MOI 0.1에서는 4시간 때에 KFS-SM의 용균활성이 가장 활발함을 확인하였고, MOI 1, 10, 100에서는 2시간 때 용균 활성이 가장 활발한 것을 확인하였다.
<실시예 6> 박테리오파지 KFS-SM의 흡착능 및 1단 증식 곡선 (One-step growth curve) 분석
살모넬라균을 640 nm에서 흡광도 값이 0.353이 되도록 배양한 뒤(108 CFU/ml), 박테리오파지 KFS-SM을 MOI 0.01로 넣고 상온에서 배양한 뒤, 100 μl 시료를 0, 2.5, 5, 7.5, 10, 12.5분에 1 ml씩 채취하여 TSB 배지에 희석한 뒤 플라크 분석을 통해 상기 박테리오파지의 흡착능을 평가하여, 그 결과를 도 6에 나타내었다.
도 6에서 보는 바와 같이, 상기 박테리오파지 KFS-SM의 접종 후 12.5분 이내에 박테리오파지의 91.8% 정도가 살모넬라균에 흡착하였다.
또한, 살모넬라균을 640 nm에서 흡광도 값이 0.353이 되도록 배양한 뒤(108 CFU/ml), 4˚C에서 1분 동안 16,000 g로 원심 분리하여 세포를 침전시킨 후, 0.5 ml의 TSB 배지에 희석시키고, 박테리오파지 KFS-SM을 MOI 0.01로 넣고 37에서 5분 동안 배양하였다. 배양된 혼합 시료를 13,000g에서 1분 동안 원심 분리하여 얻어진 펠렛을 50mL의 TSB 배지에 희석시키고 37˚C에서 1시간 동안 배양하였다. 배양 도중 5분 마다 시료를 채취하여 플라크 분석을 하였다. 또한 eclipse period를 구하기 위하여 채취한 시료에 1% 클로로포름을 가한 뒤 플라크 분석을 하였다. 분석을 통해 상기 박테리오파지의 1단 증식 곡선과 eclipse period에 대한 평가 결과를 도 7에 나타내었다.
그 결과, 도 7에서 보는 바와 같이, 1단 증식 곡선 결과 burst size는 2.5 × 101 PFU/감염 세포, latent time 은 25분, eclipse period는 5분으로 나타내었다.
상기 결과를 통해 본 발명에 따른 상기 박테리오파지 KFS-SM은 살모넬라균에 빠른 시간 내에 흡착할 수 있고, 높은 burst size를 나타내 항생제 내성 균주의 용균 효과를 발휘하는 것을 확인하였다.
<실시예 7> 박테리오파지 KFS-SM의 전체 유전자 분석
상기 실시예 1에서 정제한 박테리오파지 KFS-SM의 전체 유전자를 분석하기 위하여, 박테리오파지의 DNA를 Phage DNA Isolation Kit (NORGEN Ltd., Canada)를 사용하여 추출하였다. KFS-SM의 전체 유전자 분석은 추출된 gDNA를 MGISEQ platform을 이용하여 분석하였으며, MGI사의 Whole Genome Sequencing 라이브러리 제작 Guide에 따라 수행하였다. Open reading frame (ORFs)는 COG, SwissProt, Pfam software를 사용하여 분석하였다.
신규 분리한 박테리오파지 KFS-SM의 전체 유전자 서열은 서열번호 1로 제시되어 있다.
유전자 분석 결과, KFS-SM은 이중가닥 DNA를 가지고 있으며 전체 유전체 크기는 116,343 bp의 크기(서열번호 1)로 이 중 GC 함량은 40%를 차지하는 것으로 확인되었다. ORFs 분석 결과, KFS-SM은 특징적인 기능을 가지는 212개의 ORF (open reading frame)를 가지는 것으로 확인되었다.
<실시예 8> 박테리오파지 KFS-SM의 병원성 균주에 대한 특이성 조사
박테리오파지 KFS-SM의 살모넬라균 이외의 다른 병원성 식중독 균주에 대한 용균 활성을 확인하기 위해 특이성 검토를 수행하였다.
구체적으로, 살모넬라균과 기타 병원성 균주 38종을 각각 배양하여 그 배양액을 수득한 후, 각각의 배양액과 박테리오파지 KFS-SM을 이용한 소프트 아가 오버레이 방법을 통해 용균반의 형성 여부를 확인하였다. 결과를 표 1에 나타내었다.
균주 명칭 용균반 형성 여부
Salmonella Dublin O
S. Enteritidis ATCC 13076 O
S. Montevideo O
S. Newport O
S. Panama O
S. Salamae O
S. Senftenberg O
S. Typhi O
S. Typhimurium ATCC 14028 O
S. Typhimurium ATCC 15812 O
Aeromonas hydrophila ATCC 7966 X
A. hydrophila JUNAH X
A. hydrophila SNUFPC A10 X
A. hydrophila SNUFPC A11 X
A. hydrophila SNUFPC A3 X
A. hydrophila SNUFPC A5 X
A. hydrophila SNUFPC A6 X
A. hydrophila SNUFPC A8 X
A. hydrophila SNUFPC A9 X
Bacillus cereus ATCC 13061 X
B. cereus ATCC 14579 X
B. cereus ATCC 1611 X
B. cereus ATCC 21768 X
Escherichia coli ATCC 15144 X
E. coli ATCC 700599 X
E. coli ATCC BAA-2192 X
E. coli ATCC BAA-2196 X
E. coli O157:H7 ATCC 10536 X
E. coli O157:H7 ATCC 43895 X
Listeria monocytogenes ATCC 7644 X
L. monocytogenes ATCC 19116 X
L. innocua ATCC 33090 X
Shigella boydii NCCP 11190 X
S. flexoneri 2457T X
S. sonnei ATCC 9290 X
Vibrio parahaemolyticus ATCC 17802 X
Y. enterocolitica ATCC 55075 X
상기 표 1에 나타낸 바와 같이, 박테리오파지 KFS-SM은 총 9종의 살모넬라균 혈청형(S. Dublin, Enteritidis, S. Montevideo, S. Newport, S. Panama, S. Salamae, S. Senftenberg, S. Typhi, S. Typhimunum)를 제외한 기타 28종의 균주에 대해서는 용균 활성을 보이지 않는 것으로 보아 살모넬라균의 다양한 혈청형에 특이적으로 용균활성을 나타내는 것임을 알 수 있다.
상기 실험으로부터 본 발명의 박테리오파지 KFS-SM을 이용한 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 살모넬라균에 대해 특이성이 매우 높았고, 용균 활성이 우수하며 물리화학적 환경에 강하다는 장점을 가지고 있음을 확인하였다. 또한, 본 발명의 박테리오파지 KFS-SM은 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서 본 발명의 박테리오파지 KFS-SM은 양식 산업 및 축산업에서 살모넬라 감염증을 효과적으로 예방 및 치료할 수 있다.
이상에서 살펴본 바와 같이, 본 발명은 상기에 언급된 바람직한 실시예로서 설명되었으나, 본 발명 분야의 통상의 기술자들은 동일한 기술적 범위 내에서 다른 구성요소를 추가, 변경, 삭제 등을 통하여, 퇴보적인 다른 발명이나 본 발명 사상의 범위 내에 포함되는 다른 실시예를 용이하게 제안할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 본 발명의 범위를 한정하는 것이 아니다.
이하 본 발명의 박테리오파지 KFS-SM을 포함하는 감염성 질병의 예방 또는 치료용 약학적 조성물의 제제예를 설명하나, 이들 제제예는 본 발명으로 제조될 수 있는 제제예들을 예시적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 제제예에 한정되는 것은 아니다.
<제제예 1> 의약품의 제조
<제제예 1-1> 주사제의 제조
박테리오파지 KFS-SM 1 × 106 PFU/ml
주사용 멸균 증류수 적량
pH 조절제 적량
통상의 주사제의 제조방법에 따라 1 앰플당(2 mL) 상기의 성분 함량으로 제조하였다.
<제제예 1-2> 액제의 제조
박테리오파지 KFS-SM 1 × 106 PFU/ml
설탕 20 g
이성화당 20 g
레몬향 적량
정제수를 가하여 전체 1,000 ml로 맞추어 주고, 통상의 액제의 제조방법에 따라 상기의 성분을 혼합한 다음, 갈색병에 충전하고 멸균시켜 액제를 제조하였다.
<제제예 1-3> 정제의 제조
박테리오파지 KFS-SM 1 × 106 PFU/ml
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mg
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조하였다.
한국 생물자원센터(Korean Collection for Type Cultures) KCTC13880BP 20190711
SEQUENCE LISTING <110> Kyungpook National University Industry-Academic Cooperation Foundation <120> Novel Salmonella-specific bacteriophage and antibacterial composition comprising the same <130> PN1906-298 <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 116341 <212> DNA <213> KFS-SM whole genomic DNA <400> 1 agtcgatttt atcgttagaa ccgccagtag tttcgcccca agacttagcc atattttatt 60 tcctctttaa agattatctt cgatttattg attgcaatta gtgggttagt gtcgattaca 120 gaacgtggta tccatatggg aacatattgc atgtccaaac tcggatcgtt tgtaaactta 180 tattcggcat aatttcgtag acttagaatt cctagatatt ctgccagttg tctattagac 240 aatttgtaag ggttatcaac aatagttgat tcattcaaga tgaatgaaga accgaccagt 300 aattggtgag catcaggctc tgcaagcatt ctattaaata gcttgacaat caggtcggag 360 tttcctctag ctaagaggta aattctctcg taatcaaaga atttcatttt tgtctctcat 420 caatttatgt atatattata cattaaatcg ggaggatttg acaagtacaa tttttatttt 480 tctgcttcgg actttcttat caatccgaaa ctttctctct caaatttatg tgtatattat 540 acaataatta tgagcagttg ccaaatgcaa ttttagctaa ttgtactgct ttctccggag 600 tcatagtaat agttttccat gcattattgc gatacacggc catacgtcca gaagcctgtc 660 ttaaaccagt acctcctttc ataattaaat ctacaacaat cggatctagc tttccgtcaa 720 caatacgttg aacgcggcct gctaactgtt ctatgagaga ttcattatta atgagacttc 780 ccattattaa acaagacagc tcgttaagag aaataccttc tgagaagata ctctgagctg 840 ctgcaagcac acagggtccg ccctccgcta catctttctg gattctcaat cggtcatcca 900 aaggagttgc acctataatc tcataggttg taacaccacg ttgtgcgaga gcttctaata 960 ttgtttggat tagttccgtc ctatcactta caaagagaac cttatgccca atagacacat 1020 ataaatgtgc taggtttata atagtttccc gatattcagg atgattatat acatcattag 1080 cacgtagtgc ccacggtacg ttctggtttc ctgataactc gacaggtacg gagtacctat 1140 ggattgtggg tggaacagtg ttattaacag ggggactaaa gatcttatat ccaaagaaat 1200 ctttgaacat aacttgtagc ccatctttac gttttaatgt acctgataga ccgatcttgt 1260 agcgagcaca tgagatctct aggaaattag tgaaagttgt agctacacag tggtgaactt 1320 catcaactat aacagtaccg aataccttag aaagattatt cgcatgtttg ttcactgttt 1380 gtatattact gaccacgatt ggtggatcaa tgttatactt tccagaacct ataatacccg 1440 gctcgaatcc aaaccattta cgaacttctg cagcccacat ttcacgaatg gatgtgttag 1500 tgcagattac cagagttttc tggccaaact tatatgcaag agctagagct aggatagttt 1560 taccaaatcc aggcttacca ttaataatac aggtatcatc gcactcttca tatataggaa 1620 gctgatcttc ttcacgtagc ttgaactgag gttctggtat ttctaccgga gctaatgtgc 1680 gtttatcaac taattcgtat tttactccct tagcatctag taaatctaga cgcgtaatgg 1740 gaatccactt aatctcttta gcaacaacac cactattctt atacataata gggtatttac 1800 tagtcattgt ctcgatgtga taagtggttt gcttactaca ataatcccag agttcatcat 1860 cgggcttgaa ataggcttta ttagatataa caaccttcat aattttattc taagtctagg 1920 aatttcaggc tcctcttgat ggacttgata aataacaggg ctattattta ccagaatata 1980 gcttatataa gctggaacat aggatagcac aaatggatac gggactttag ccacatagca 2040 ttgatacttt ccattataaa ttcttgcaga atgcagaact ttagacgtga taacatcata 2100 gaacgtagtt ttcttccagt taattatatt tccatcagaa tctatgaact ggctacgctt 2160 tgacccaact aattgggaca acattgatat tctaccacga atagggtaga gcttataagg 2220 taattctttc cgcttctcaa ataaggctaa cctacgttga gagaatgtgc caggcaactc 2280 cctgttatct agcacatatt tattatatct tgttgtaatt acggaataat caccttcctg 2340 ctcaattgat acaaatgccc gtaaagcata tacgggcaat ttgaaatcaa gcacctaaaa 2400 tccttttcac attatccaga tctttacata cagcaataaa cttatcatcc ttgtacttgc 2460 tgtggtctgg atgctcttta tccattgcag ctaacttctt atactcgaaa tctgcatcga 2520 gtaatatacc cttaacataa cgagtatatt catcatcatc aatacaagcg attgatgggt 2580 gctgtttctt catcttacca caagagtagt cacgagaacc acctttctca gaatcagaat 2640 caataccgat tgggcagcca gggatactaa taccacgatc cttctggatg ttacgaatca 2700 gaatctcgtt atattgatca atcagatctt cacgtacaat agcaaccacg gagtcgtgaa 2760 ccaacatgac gatcttcatc tcttttccta gacccaggga caggatttcc tcatccgcat 2820 caatagcacc taacaacagg ctatcagaag atgcagactg aatgattgcg ttaaatccag 2880 aacggatttc ttcaccctga acaccacgat cttcggaatg gatgttgtgc agacgacgct 2940 tacgaccaaa gtgactatag atgaatccaa agttcttgat ctgatcgtgg cacttatcaa 3000 tccaacgctt aagctgaggg aactgaccga agtaagtttc gatatagtcc ttcgcatctg 3060 caacggtaca ttcaacaaac ggttcgcctg tcttagcagc ctgttccaga agagcttcgt 3120 taacagaatg agctacttta gctgggccag aaccatacag aataccgaag gtaattgcct 3180 tagcagcctg acgcagagct gggaacagct tcttaacatc acgtggctca caggtcagtt 3240 taaataccat gtgcgcgatg tttgagtgga agtcaggata tttatcaggc tcattcttca 3300 tgttgataaa tacttgttgc atatttctgt caccagacag aacagcagca tagtaaactt 3360 ccgcagttgt taagtcccat gcgattacac gatatctagg aggagctact acacaacctt 3420 tgataataga ttcatcacgt ggtaactgtt gcaggttcag cttaccagaa gaacttaaac 3480 gaccagaagt agtcatgtgt tcatggaagc cagtacgaat gcaaccatct gcatcgatgc 3540 ttagaagaat cttctcaata taagtagaga tcagcttagt cagcttacga atctctaaca 3600 gagttttagc aatcgggtgc tgagtagcca gttcgttcag agcttctgcg tttgtagaat 3660 ctgcacctgt atccgtcagt ttaccagttg gagtcagacc aacataatca aacagaagaa 3720 cacggagttg cttaacagag ttcgggttaa acgcttcgtt ctgatctttc tctagctgaa 3780 cgacttctgg ataagtatac agcttctcac gagccttatt cagattgtga gttaactgat 3840 actgagcttc tttcaagcga tcaacagaaa tcggtacacc acgatcttca acacgttgca 3900 ggaatacaca accaggcatc agaacatcat aatacagact gcaaagcttt tcgttcttct 3960 caatttttgg taagaagaag ttatgcagac gcaaagtagc atcggtatct tttgcagcat 4020 acggccacat aatatcaaat ggaatcagat catagctgaa atcctctttc ttgattttat 4080 gtgctttgca ataatcatcc ttaaacttat ccagttcaaa gtcgtaatcg cccatgtcag 4140 tatatttcat agctagagat ttcaagccgt gagtaccacg acgttcatca agaacatagt 4200 gctgtaacat ggtatcatga agtctacgct ctttatgtgc tttctcaaag gaaagtccta 4260 aatgatagga ataaaagtgc atatcgaact tcaagttatg aaaaacgatc tgatgattct 4320 tgctatccag aattttctgg agataatgta cagctacttc ggtgatgctg tcagagtcaa 4380 tataaacacc ctggtactct tggtgagaca tagaaacacc aagcagataa ccatcacgag 4440 cgtacaaagc tgaggtttcg gagtcgaatg cgataggacc gataaccatg ttatacacca 4500 tcttgatgta ttcttcagct tcatctggat cagtaatagg acgataatca ccagcttttg 4560 caatcttctc acgaccatta ataatatcgt gaatactttc tacagttgca tcgaaaactg 4620 gtttcatttc aggtttaaag tgtaactgag ctggactaat acttgcaatc caattggcat 4680 agccgccatg ctctacacgt ttaccagtat aatcaccgat acctttctta cctgcgaaat 4740 acaggaaagg ttcagcacca actagtacaa caaaatcgta atcgttcgga tcgaacgggt 4800 tatctggagt tccgattgta atatgctttt tcagcaaacg gccagataac ttctcgttac 4860 acatatggaa tacatcaact tcctcaccat aaagctggaa gtgtttatca taacgagtgt 4920 tatttagagc tttatcaact actgcaattt tcaaatttag tctccttttg gtaagtaagt 4980 ataacttcag tgtttcttct ctaacttacc aatatattat accaaatctt tgagcgattc 5040 ggcaactaaa atttcaatac gttgagccaa catatcgatc tcttctttat ttaaatcacc 5100 tgggtctttg ccttctggaa gaaggaagtt accaactaca ggtgttaaac gcgtttttgt 5160 acgaattagc ttcgctagtg cttgtgcggc tttattacca gaggcatcat tatctaatag 5220 gataactact actcttactc cagcaataat gtaaggactg aacttatcgg caatgttatc 5280 agatgtaaac tgatgtgtac caaagcagca agaagcataa tccacaccgt tatcttctag 5340 atttaacata tcaaagatac cttcaactag aataagaact ggtgtattat atcgtacagg 5400 gaaaatcggt ggtgaaactt gttttggttt tactaagtat ttaggagggg cagaactgtt 5460 tatagaacgt cccaggaata ggatattgcg tccaacagca tctgttattg ggaatacaat 5520 tctgccttcc cagtctgctt gatgttggaa agcaaaatat ttcttcaagg tcttagaact 5580 gatacctcgg aaatcacctt cgaaaaggta agcagattca ggaattgcaa gattcgtaga 5640 tccattccta atctctgaaa tctttctacg tacttgtgac aatcttggtg actgtctgta 5700 ctgagtctca ttaaaataat ggtaaatgct tggtattcct ctaccgaagc cacaactcaa 5760 gcagtgcata atacctgatt caggatcaat acgcaaactt gggtgtttat catcatgatc 5820 tggattgaga caacagatga ggatgtcccc acctgtgtct ttgtattcaa tgcctttcag 5880 atcaagtagt tctgttattc tactcatata tcgctggcct gttcacctgt tgaattttca 5940 gctttgtctt tcttagcacg tttaggttga gctggtttat ccttttcaat aggaataacg 6000 aactcagctt ccatttgcgc aatatcttcc attgctaggt tggtagtatt atccattcgc 6060 agagtttccc agttcatctt aggcataaac ttcacactat cagaggaacg agtcttaacg 6120 aagtcaaaca taatagcacc ttgaccgtta tcagcttttg cagcattaag attagcagcc 6180 atatctgcgg aatcaagaat ccctttagac atacgcgttc taccatcttg gtcaatctgg 6240 taaggggcaa caccagccac gttatgtttc tggcagatag atttgaaaga tgaactaaca 6300 accatctgtt ctttccagtc atacatgtca atggtctttg aatctggaag tcttgtttgg 6360 ttaatatagt ctagaagagc tacagtaacc ttatcaccat atttagcaac tagtttattt 6420 aattctacgt caaccgtcgt aatagacagt tcaggatcgt aaacgatgat cataggagtg 6480 tgaagctcat agccttcaag gagtttactt tccatatcat agaagtcact cattttagcc 6540 atagtgtatt gcttaacgaa gttatcgaaa agctcttcac caccattaaa cattctagcc 6600 ctagttctgg ctaatctcaa cagagcagca ccttcaagag tgttattacg cattgctaat 6660 gcagatactc cagctaacat agccagatta cgtctaaaca cttcgtgctc tttcatctca 6720 attgagaaat atggagcaat gtctccattc aaatattgct gaacctgtat gtttgaacag 6780 ataatggatt taccagtacc acgccaacca ccaagcagta acgtttctgt gcgagctaaa 6840 ccaatttgag cgtcgaactc attacaaata ccaagagcga tcaagttcag tttggtatct 6900 tcttctctct ggaaaatacg cattgtgtct gcgttgaata cctttccagt attcgttact 6960 ttctcttcta attttaagtg aagggaggca actcggttga gaatttctcc ctgatccagc 7020 attgttaaat cttgaagcac gtctgtttct agaagtttca ggaatagatc ctgtgtatat 7080 tcggcttcta ggacttcaag cgcctgttcc atgctcactt ctggaatttg agtgttagct 7140 aagacgacaa gagcttgaga aaggcgggcg ttcctattgg cctcaagcat cagtgcgtca 7200 atggacggca ttgcgttata tttcttataa taattctgga cggcttggta aattgaggag 7260 aaagcgtcat taaaatgatc tttatgcagt tttgagaatg tttccaatgc tatttgcttc 7320 tgttcagaag cgagaagcat cttcaacact acagcttgca cgttaaacaa ggtcattctc 7380 ctttgcacgc tttcgtgcct tctaaatgca aaaaggggaa ggagcatagc cccctcccct 7440 taggtttaat ttaccagatt attcagcagc tgcagctttt gcatccagtt tagcacgctt 7500 agcggcacca tcatagtcct tagcaaccag accacgacga gacagcatag atttaacacc 7560 gcgctcagac ttaccagttt tctcagcaac ttctgcaaca gtcatagaag ccagatccag 7620 accttctaac agatcttcac gagtcttagc acttgatgtt tcctgtaccg gcatagcagc 7680 gatacgacct tcacgaagca ggctcagagc tttaccacgg atctgcttaa tattacgacc 7740 gaaatgagca gcgatagctt caatagtagc accagcaaca acctgattaa cgaaatcagt 7800 ttcttcgtca ggagtgaagg aacgaacagc agcagctttt tcagttggct taacagaagc 7860 agtcatttcc agactcagga tcttaccctg tacctgctta gcaccgaact gaccaccagc 7920 tacagcagca gcgatttcag cgtaagtata ctgaccagca tgagcgttca ggaaatcaac 7980 tagttcagct tcctgctcag gagtccacgg ggatttctgt acttcgttag ctttctgtac 8040 ttcgaaacct tctttacgca gcttagagcc aacagagcga gcggtaacgt ctttaccagt 8100 ctcggtagcc agttcagcag cgatagcagc tacttgttct tgagagatta cagcaacacc 8160 cagagcagtg gcttttgctt tcagagactc ggttacacct tctacgttcc agttcagttt 8220 agacattatt atttttctcc aatagttctt taatcgacag gatttctatc ccattcgttt 8280 cggctttctt ataagatgag gaagaacgtt ttgattcatc ctcacagatt aggtatttga 8340 cgtctttggt aacggacttc ttaaccgtat atcctagact ttctaaatag tttgttgcat 8400 ccgttcgatt tgcaaaatct tgcaaagatc cggtaataca gaccgtgatt ccatttggct 8460 gggcaactaa atcatctgta atgatgatat ccgctttagc acctcgaata cccgttgaga 8520 atttccacgg taattcaata acatccttgc cttgtgggga atttagccag gttttgtaat 8580 tttctccagc cttgccgtca gccttcacat cgtggaagct agtgcaattt tgggacagtt 8640 tctttgctgc aacctctcca ataagaggaa ttcctaaaga accgagaact gaaccaaagt 8700 caatgtctcc gcgaacctta gtatttaact cgctaatcaa tttagcggca accttgctac 8760 ccacagctcc gaccaaatct tcttcggtca ggtaaaacag ttctgagatc ttagtcagct 8820 ccagcttctc aatagttttc gggccaaagc cctttaactt catttttgtg cagaagttct 8880 cgattaactt acttgattgc gctggacagt tggacttatt cctacagaat aattgcccgt 8940 tgacaagatc tagcttagaa ccacaagagg gacattgtgt tggaatttcg attttcatca 9000 aatttcttcc ttatcaattt atataaatat tatagcaagt atttaagcat ttagcaacta 9060 caattttaac taactttgct taccctcgcc ataactttct atccctcaac tgaatgataa 9120 tagtatatac ctaagcggcg aaaatgtcaa taaccacttt ataattccga catggtaaac 9180 gggtagcaaa gggttacgaa ttagtcgtat actcgctcta ctatacaagg gattacacca 9240 ccagcacgaa tcactcgaat ctggcaaccg atctctaaat cgagagcgtt aatataatca 9300 acgttattaa gagttgcttt aacgattgtg gcatcatcaa taatcaccgg ttcgaaataa 9360 ccaactggag ttactttacc agaagcccct acttgccatt caactttaac aagtgtggta 9420 atttcgcctt cttcatcctc tttaatagca aatgcaccac gaggaaattt attagtccaa 9480 ccttcacgga agaatttatt gttgtcgtta atgcgaacaa ctttaccatc tgtcggaatc 9540 catttaaaga aggaacgaac attaataact gtcaagaagt tctcattctc tagccaaagc 9600 atatctttta aataggcttc tgtaataccg actgtttccg ctgaacactg gataccatat 9660 gcaacgaaga ttaaaccacc ttcaccaata cgttgcatga aatcatcact atctttcagg 9720 ttaatagcac cagaagcata gttacgtttg ttctctactt ctttagtaat gagaacttca 9780 ccagtgattt gagttggtac tttttgggaa atagttttgg ggatgttcag cattttaaca 9840 ttatgcgtta catcattccc tagaataccg ttaccacgag ttaacgcttg aacaaattca 9900 ccattaatat ataatagaga aatcgcacaa ccatctaact tatcagtttc aacctgacct 9960 aatgggttaa atggagcttt atcaccacga tcgtaataga ctttctgtaa agaatacata 10020 cgatacaaat gagggatatc acctttcggt ccgatttctt cctctagtgg gaatctttta 10080 atcagactat catattcttc atcggaaata agactcattc cctggtagta cgcttcttga 10140 caacgtttga taaagtcttt tacattattc atcgtctgcg atttccattt tgagttcttt 10200 attatactga gcagccatac cagctaaatc agcagcttct ttcaaccacg catctttgta 10260 cgctttctgg tttggagtgc cgatactatc ctggtaattg attcttgcta tggagagcaa 10320 attaagtttt gcttgaattt ttggaagtgt tattgccatt tactgtacct catatgcttt 10380 aggctgaata gtatccccct gccatgtaaa gttcttacat aagcaaagat aggtagtcct 10440 tcccactctt tgagttattg gagagtaaat atcagcaact aatgtgtttg cttcgtaagc 10500 gagagatata agtttatatc cctgtcgaac catacgtacc ttctctccat tgagcttttc 10560 ttcccaggaa agaactaaag actgccctat gtacggtaaa ggtttgagtg tacttatttt 10620 tatctttttc atattcaccc ctctcattaa tttatataac tattatataa gaaaataggg 10680 atgaaagcaa gtacatttat aaagaaaaaa gccaagaact aggcattctt ggctttgagt 10740 tcttcagctc gtttctgtac ttcatgcaag acctctgatt cactgaggat ttgcgtaaaa 10800 gcgtagaata gctgtgatgt agtttctagg gtataaggaa aagagaagcc ggatttcgtt 10860 ggaaaccact ctccagtaat atcttggagc cagtaacgaa ttcccatgta tagattccca 10920 cgaaattcag atacggttag tcttacttgt tccccctcct tctcccaaag catgatggac 10980 tggtcgtcaa cgtgtccttc atagttttga tttacctgat cgctcattta catatcccaa 11040 atacacaaaa agccccatat aggggctaat tggtttctta tcggcctact ggagaagcac 11100 gatctaactc tgactggagg ctcgtcacac ggcgaacttt cgcaacagga atgaaacgga 11160 agctatcgtt agtacgagag aaaacgagga tttcatcttc tttcggctta cggatgcgct 11220 cacgtttaat acgctcagcc aggtatttat cctgagctgg atcaaattcc attgtaccct 11280 gaagatacgt ggttcctttc tgacgaagtt tttcgtaatg aagataaaac tcaccaaatt 11340 tttcgcattg agcaataatt tcagctttat tcatgactgt attccttgtg ttggtggatt 11400 tatctaagaa ttacttagtg atagctcgga tagcttctgc tagatgagca gctgctttac 11460 cagtcagctt gtcaatgatt gcgtcgtcca ggaagtccgg ggctaagcca gcatcagaga 11520 atgcagagcg aagatcggag tgagcctgag ctttggaagt acgagaacca ccagaagctt 11580 tttctccagt agatgcgctt gatttagaag tagagccagc agctttcttg atatacagac 11640 ctgctttggt cagcttcata cggaaaccgt tcggagttac accattttcc tgagcaattt 11700 cgctaacaat ttccatgcta acgcccggac gctcatcttc tgggaattgc tccatacgag 11760 caacgtagtc ggaagacatt ttttcgaaca gttcatcagt ccactgagtt ggagtagtca 11820 tatttttcga tttccttaat taagaattaa acagaatagt tctttcaaac tatgagaata 11880 ttatatcaag aattacagga ttaagcaact gaaatttttc agtagttact ccaccaggcc 11940 aacagcaagc atatcatcaa tgctggcaat ctcccaatcg tcggcaatag cttcgcacac 12000 tgttaacgta acaacctcac tatagggata attaccagct acccaggtat gttgagtaac 12060 tacatctgag tcagcgtcta aagttattaa cactaagcca ttattgtcat tatcccagct 12120 tctacgatat agagttttta cttccccatc tttaagcaaa tctgttaatt tactcactgc 12180 gtcttccttt ttctttatca agtttcaggg cagctttaac agcttcattg actaatgtga 12240 ttacttcttc acggctatac ttgaatccta aagccttaac atcgactccc agctcttgca 12300 ggtgttttac agaagctaac tcgtaattca tatagtgaac gttctgttgt ttaccttcac 12360 ctagtaacca aatacgataa gcccctactg gattagttaa tgctttctta acttcaccaa 12420 tacactgata accaggaacc caaacaagct caccaacttc aaattcttct gccattgcat 12480 catctggaac cattggggga ctcaacggat ccacgatatc gttaattcga agcagggcac 12540 cataacgttc cagaacagat ttaatcatcg ctacagaacg atagttacga tctgcgattt 12600 cttcaaaaga atcaccagaa agatactgtt caattacatt tgcaagctca acaccttcaa 12660 tcagtgttcc acgcttcttc tttttcattt ccgcaacttg aacctgacga tctctccatt 12720 cttcgatcat tcgttccata gttgggttag aagaaacacc gagcatttca catgctgctt 12780 tcttagtacc accattctcc agatgttcaa taaccttttt aaaggtttca tcagggattt 12840 catggataag tttctttcta cgagaaccag ccatatttaa actcctctct caaatttatg 12900 aatctattat acagaaaatt tttcctgaaa gcaaataaat ttttaagctg gatatagctc 12960 ggctttgaaa gcagctctaa cttctatggc ctcttctaac gaattgcatt gttggtactt 13020 acgagtattt cctttttgca taagtacaca gtatttttca ccgcgcatat agatattaga 13080 ttctcctaac ttattgtcct ttctcacatc tatattatgc atttgatcag atctattgga 13140 atcttctaga ttctctatga aattacaatc cttaattctg ttattatggt ctatctcttc 13200 aggaatatat cctttatgat acagaaagac taactgatgt tctccccatt tcttgccttt 13260 gaaaccaatc ctgatatacc ctttgtgctt cccacccgca aactgcccta ttaacctctt 13320 ttggttagtt ttcctccatt tgaggtggcc agtttctgaa tcatattcaa aggcttctct 13380 aacctcttct tgagttatca tcttctttaa ttaattctcc taattcctta taaacacgtt 13440 ctaactcaaa agggatacgt aaggatattc gtaccttacg ttgttttaac agccatatag 13500 atacgttcgg aataccagcc aatctgggag ggaccactcg gcatagtttc tccgcagtat 13560 cgtaaacaga agttttacgt tcttttacgg gaaacttcct tgtttcccac tttttattct 13620 tcactatcat ttctagctcc caattcagaa agagcttcct cgtgcatctg acgttcttcg 13680 tctgtcataa acttaactgg aatcggcata cattcgatct tacagtaagt tctgtaccag 13740 tccacaatct tttccgtatc cagatcttta ccgataccca tcatgccaag atacatacga 13800 gcatattttg ggttagcact ctgacctgtc ttaaggaaga aatccttctt cttattctta 13860 agagcttcga taaacggttt gattgttacg tttgaacact ttttaatatc ttcccagaac 13920 atgtcgtgca tcttatggaa gaatgcagct ctatcgtttg gtttatcctt gatatactgt 13980 tcaacgtgtt ccactgttac atctttaata gaatccagat gataatagcg aaccagaagt 14040 aacttggctt cataggcatc tgcatgacgt ggtgcacaat attcaggagt tgagtgcaaa 14100 atagctttga ttcgctcttc tgtatactct ttgtctgcac cttcgatagt tccccagctt 14160 tcgctgaaaa tatcgatagt tgcaccctga tcaagcagat aataacgcaa agtactagta 14220 tagtttctag attccagctc acgacgacgt gcgtacagtt gttgggctaa ctcaatccaa 14280 ccttcgtcaa tattatattg ctcggaaccc gcagaaaatc cactcgtaga tttatcaata 14340 aaataataaa tgttctgagc accacggtcg cgcctgattg cttgaaaacg catgtttggc 14400 gcttggtttg aggttctagt gattacgaat acgttatcaa aatagttaaa gtcaacacca 14460 ctcgttacag atggactaca taacaaacaa tcaatctgtt gatcaattag ctcattagtt 14520 gtataatcca gaatacgacg gatatctaca tcagacgtag agtttgagtg aatttcttta 14580 actaacgcac ccgtattacg acgtaatgcc attccctttt cattcagttc atctggtcca 14640 cagtcagata ctaggattga cttctcaccc atctctagag atgtttgaag tgcaacccaa 14700 atactggatt cgtcagggaa ttcataagca tgagcttttg ccagcatttt acggtgatga 14760 ttatagaacg caaccggttt atcaaaatca atcaaagaac cataggcttc aatcgtttct 14820 gcactgatat ccccatcaga taagatcacc gtttttgcag taataagaat atcacgaaga 14880 acctgaatac attcacgacg gtttttaaca accggagcga ataacagatc attcattact 14940 gcgtcacatt catcaataaa gatcgtatca atctgaccca caaagctctt gaacttatgt 15000 atagagtgaa tagttgaaga catacgatca atagcaccac gtttgaagtt cagcatatca 15060 actgacttat catactggcc agcaccaaat ttcttcgcgt tagaagatac aagtgcccta 15120 gtatttgtga ttgcaataaa gtttcctgga agaacacctg catctagcca cttagttact 15180 gctgtagttt tacccgtacc aagacttgcc ttaacaaatg ttaaatgtcc ttttggtggt 15240 acaatgttaa ttttcaggta tggaacttct ggtggagaat ctgtgtctag acgttttaat 15300 ggtacacctt ttaagtttgg tggaatctca cgtttggagt tatttacaaa ggctttgaga 15360 gcctgcttac gaccgttatt aaagtaatct tgaatattac gactattatc tttagttgca 15420 atatattcag ataaagctgg tttaatctca cgttctaacc acgtaaagtc aacaccatct 15480 tctaacgctc tgtgataaag ttttgggata atacgcaggt acaccccatc ctcagcttct 15540 tctagttcgc tgatggtttc ttccacttta tcagatgcag ccttcttgcc cttaatttgg 15600 tcaagtaagg agtagaattc ttctttaaac tcacctctag ttgtttcaaa gtcaactagg 15660 ttattgggta gattcacttt cgaaccctta acatacacaa gacgtggttt attctccgct 15720 ttaaacggat cgactatacc atcatcaaat aagggatctg cgaaatagtg aagctgaaca 15780 gaagaataat aagctaagtc agcaatatca aaaccatact tttgcttact actgtcattg 15840 atagatgtaa ataagaactt taactgtccc tgcgttacct tgacattaga ttctagcatt 15900 aagtgcattc ggatacctgg ttttaatcca gccgaagacg atgcatgggc aataaaccct 15960 gcatcaagag gaaacatatc ctcactaata ctattcaaca tcttaataat atgacgtgcc 16020 atgccgacaa gattaaactt gtcatagcca ccagtatcta caataccatc cacgtccata 16080 gcaataatat gactaggatt ggatacatta aagtttcctc tggtacgacg cacattatct 16140 tttggagcta aacatttacc acgaatagct acaatatgcg ggtctttggt aagacgacgc 16200 atgataggta acatttcagc aaggttttgc gggtcaagtt cttcaagtac atcaaatttg 16260 aagggcatgg aagctggttt accttctgga tgctctttag agaatcgctt tgcaaaggtg 16320 tagtcttcca ctttgatctc tttccagatg ccagtggctt tatctctgga aaatcctgcg 16380 tgaccttgta ggattgagaa cacaacaata ctcctatgtt ggttattcgg atatctttct 16440 ttagggctat tcaaaagaat agaacaacta ttactaccat ttacacaaac attaatcatc 16500 atcaagcagt atgaataagt tatcatacct cagtgctgcg ctctcttaac tcttatccag 16560 atcagaacct aggtcattgg ccgctaagcc gattctgatt gccctcacta tcctattggt 16620 aggtggctac tggacgtgtc accattcgct gctcttttcg ttcgcacccc catataaagc 16680 tctgcctata taacgagcta aatatgcacg gtttggattt acctttccga attcagggta 16740 cgcagatcac ttaattgata attaactaat gcagtataaa cactagtaat agttaaaaca 16800 acccttaatg gggcagccta actgttccgt attgcggatt gtaaacaaga aaaattagca 16860 aaatcaattt ttactaatgt ctctcattta tgggaatatt atactataaa ataatactcc 16920 cttgcaaatg aaattttact ggattgattc cctaccgtat aattggcctc taaacatctt 16980 ggccatagaa catgcaacct catagttgtc atatgttcct ttaactatcc gtttacggtt 17040 aatagtaaat actacttgcc agtttttact accagaaggg taaacatgca ttcctttttt 17100 atacttggta tttcttagat taccagttac tgtctgatct ctaagatttt caatacgatc 17160 atcacctttt atagtgttct catgatctac caaatcaggt aagtacccat gatgcattaa 17220 aaatattgct ctatggcgaa gcatagtcct gcttcctaga gatatacgat aatacccatt 17280 acctgtagaa acacaacatt ctttccctgc atttcttcct gtagttctaa ataacttacc 17340 gtcttcatat cggaagttat ctaaaagttc ctgctgtgtg aatgacatcc tcacctccaa 17400 tacatatatt attacataat ccatccaaat tgtcaatgac aatttatttt gacaatcaat 17460 cgactggaaa gattaggtaa taatccgagc actaatagcc cggattatag agtttagtta 17520 atgaaaatca atcatcattt aggcctggga catagattcc aatgcgattt aattgacctt 17580 tagcttcttc gagaatatct tcggtgtact cccaaatatc atcttctact tgcaaaagat 17640 aaggggattc accagtttcg gggtcacgtg taatggctaa gccacgtgga tggaatatag 17700 agaagttgag ggcaacaatc aagcccatac tggacaattc attccagtca gcttcccaac 17760 catcatcttc atcttcgtcc acaaagacac ctacactagg gtctgttaca gaagtaaacg 17820 gaaatgttac actaaccttg tcaaccccca accaacgtgc aaggagccct tcagcacgct 17880 ctaccaaact aggatctatc tgacgccaaa atacttgttc cattctgcct ccttaagcag 17940 ttaaacctgg gatcatatac ccgaaacgac gaagtttctg aatcccctca tttacttcct 18000 cgggtgaata tgaccagccc tcacgttcat ttacctggaa agcaggagct tcaccagtgc 18060 gataatttac aataagacgg tgatttgcag gcataaacag cttatggttc aaggcaaaaa 18120 ccagacccat ttcatcgagt tctttccagt ctacaatctc ttctactccc tcccctaaat 18180 ccacgctaac tccaatacac ggagcttcct ctagtgggcc tttaaaagac aggaagatat 18240 cgttattctc acaccaaatc ttaagcagat tgatggtttc attgtacata ccattaggga 18300 gagaacgcca gaagttgagg agcataggat ttacctcttt catgaaaata ttattgaaca 18360 tgcttatacc ttaagtttct gaagagcctt acacatttca agatctggac taaattccag 18420 catatagctg atagcattta aatcttgaag gtttattcca gtcctgccat ttacaataac 18480 agtaggaaat tggtacatca tagcaccagc atcatctagg acgcaccaat gcttgagctt 18540 atgtttttct atgaaacgta agacagaatt acctcgagat aaaccacctc cagtaaaatc 18600 tgttgtacca aggaaacgat cgatcaaacc gagcccggtc ataatctgaa cgttctccat 18660 ctcgtttcgt acagaaaacc atgaagatac cccaacaatc attattggag aaggtttaag 18720 gaagttctga aaagcctgga gtaatggctt gtaaacccaa tcactgccaa agaacagctt 18780 ttcattaact ggagcatgat ggtgagagat gctcgaattg agcaccccat caatatccag 18840 gaaaatcaca ggagtattac ttatttccac cagtagcatc ccacgtccag actttaactt 18900 catcagtaaa aggaactgga accttcagct cacctttacc ccacataaca gatagaaaca 18960 gagaataaat ggtaatcata ccaattaggt tgacaaacca tactagaccc ttccagatac 19020 ccttaaaaat cttcttcacc atactctccc tccttaataa ggagctcttt ccattctgct 19080 gtatcctcat agataacgca gaaatcatca cttttccatg agaagtcatc ggtagcaaat 19140 tcgaaacggt agcaccaact accgtccggc catactaaaa gatcacacat gcttttgttg 19200 gacatagggt ttaccttcta gatgagcttc cacacgagca ataaaatcag gatcactgaa 19260 ggcatccacg gcacctttac tccagtaagg aacaccacgt tcttctaact gatacatctc 19320 actacgagtc ataccctggt agccaggttc catagctact gcactatcat aatagttaac 19380 taccataagc tttgcagcag taagattcag aacaggaaat agctcccaat agaagttaat 19440 tacttggtta gagcatccaa caatgatgat catatcctga ctggtaatac catcaaaaac 19500 ctcatacatt gttttatatg cgggagcaac ctcaccaaag aagataacgt taggtttaac 19560 ccaatcaaaa tcatctggat caatagaatt gtacccaata tcaataatat cgcgtgtctg 19620 gtcgaacgca tgctctacga taatttcttt cagatatcca tgaatatgga gtatatcttc 19680 tctcggcact ccagcacgtt cgattagatc atcaacattg gtagtaaggt ttaccacttg 19740 atttggatac cgtttatacc attcaccaat acgcaaatga gctaggttag gttcaacagt 19800 ttgtaactct tcacgacgtt tgttgtaaaa catatgagtt ttatggtaaa aattaccacg 19860 aaaggcatga atattacata cctcttctag ggcataatca tcccacaacg ctttaccact 19920 agcagtatca gtacggaaag cacgaacacc actatctaca ctcaaacctg caccactaac 19980 aatgattagt ctacgcatta tacgattcct tgcttgaatc cttcgatgat attttggagc 20040 agaattgctt cttctgggcg aaccttctct tgcttaataa gcatatccaa accagcaata 20100 caggtagcta ctccagcatt atacccagca ttccactgct ccatgagcat attttctaaa 20160 ttacttttta gattctgatt caagaagtcc ataaagtttt tcctccaatt ccataatacg 20220 attttcgtaa gaagcacgca gcttctcgat ggacaaagca cgaactgtaa cttcatctga 20280 attgatcgct agctgagcta gatcttccgt ttccatacgt ccgttgtaaa ttaagcgata 20340 tttaagatgt tttacgtttt tcattcttgt tccttagttc tgtactgtaa attcattctt 20400 atcacagtga gcataataat acccatacat acgataaatt ttcacagcct tagtagaccc 20460 cttcgattct tcataatcaa tccagagcca cttgtcttta cctacataat gtacccgaaa 20520 cttcttaact ttcttatcat tccatgttgc aacaacacga cgataatcaa atccgcccaa 20580 ccaatctaaa gccttacgtt cttcactagg ctctccaaga tgggaaacat ctactttcgg 20640 aagcaattcg ctcatagctt tctcctctca aatttataaa tcaattatac ataagtttta 20700 gctcgaaagc aaataaaata aaagccaggt cgactagcaa cctggctgat tcattattta 20760 gcgcgaatgt cctgatgttt cactcgaccc ataacctcat gttgtttacc tgggttaaac 20820 ggacgagcac ctggatttcc aagatatcca caaactcgac gagttacctc aagagtctca 20880 ggatcatgat tgccacaaat tgggcatttg aaaccatcct cagaagctat agtttcaccc 20940 aagaacccac attccccaca agagtctact ggagtattaa taccgaagta atgaacttta 21000 cttgcagcat agttaataac ccattccagt gcatcaggga atcgtttcat atccggtaat 21060 tccacatagg aaatgcaccc accagaagca atgggagtaa agttagactc ataatcaaac 21120 ttcacatttg gagcaacctt acgttctaca tcaaggtgat gggaattagt gtaatatcct 21180 ttagccagaa tatcttcatg ctctgggaaa tactcacggt ctagacgaca gaagcgatca 21240 catagagatt cagatggtgt agcatataga ctataaccta attgagtctc ttccttcttc 21300 tgatcagcac gatcacgcat atattgtagc acacgttgaa cgaacttgat acaagactca 21360 gacattgtat ctacatcttt accaaacatg agctgcagca tttcgtggca accaatatat 21420 cctagagata cagaagcacg cttgtagaaa tgatcgaata catattcatc tggccccaga 21480 cgtaatccaa aggctcctga catatagagg atgggcgcag ctttagcttg aatgtatttt 21540 aagcgatcaa ctctccactc caatgctttt aacgcggtat ccacacgcag ctcaagcaga 21600 tcgaagaata gatcaatatt tccttccgct tcgatagcga ttcggggaag gtttacggaa 21660 acaacgccta agttgttacg accagaagtt tcaccatctt ccgcagcagc caggaatgaa 21720 cgacagccca tagagacttt gtagtctccc gtaacttcta caaccttatc ataactgatg 21780 tagtcaggat acatacgttc agcagtacat ttcatagcta acttcttgat atcatagtta 21840 acatcaccag gtttcatatt tacgccatca cgcaaaacaa ataccagttt agggaagata 21900 gctgtacgtt tattgatacc caaaccatcc atacgcactt ctaagattgc tttctgtaca 21960 agacgtgctt cccaggaagt tcctagacca aaaccaaagg taataaatgg actctgacca 22020 ttagagttga acatcgtatt gatttcatac tctagacctt gacaggcatc gtaaacttct 22080 ttctcagtca tttccgtagc cattacagat gctttggctt catccttagt ccaacgcttt 22140 gcaaacgcat gattcttatc gtaagactta cgaacataag gagccaatac ttcatctaat 22200 cggtctacag aagtaccacc atattgatgt gaactaacct gagtgataat ctgagctgtg 22260 atagcagccg cagtagtaat agagtttggt gtactgattt cagcattacc aaccttcata 22320 ccattcttca acatatcttc caaagcaacc aggcaacagt tagtcatgcc caaaagggaa 22380 taatccaagt catggaaatg aatatctccg attgagtgtg ctgtcctgat ctgtataggc 22440 atttcttgca aggttaaaaa tttacttagt tcacctgcaa tcatatcacg ctgagtagga 22500 aaacgttctg aaggtttatt agcattatta tgctttaggg tagagtctgc aaagccatct 22560 gtaataccat agatattatc gaataattca gtttcagtca ttaatatttt cctcaataaa 22620 ttttactgct atatctggtt caatatcaaa aaactctgta gcaccatcaa aatccttata 22680 atagcagttc cattctttaa aatgctcatg cgctgctttc tcaattttac ggcacttagt 22740 atagtctaag atatcaaata ctttaactaa tttaagatct ggtatattag catttctgct 22800 aatttttcct aatctagttc ccggtttatt agagatacca atcttagtac ctaaagcttc 22860 actatacata atatatagtt ttgttttaga cctgccttct tttttacata tcgggcaatt 22920 actatctaaa tttttcactg cattaatagt agtattccac tcatggccat tttcacacct 22980 aaaaagggtc ggagtcctat tatttacata tttgcctact atttgtattc ctttattatt 23040 taattcctgg tttattacct caggagttag tttagctttc ccactacaat atggacatcc 23100 aaatcccatg ttaagtaaca ctgatggttt tgtttcccat agatgcccta ctgaacatct 23160 aaacctagtc ttagtattag catttatata ttctccaact aagacaattc ctctatttaa 23220 aagtctatta ttgacttctt ccgtactcaa tttctttggc attttatttc tcctatttat 23280 tacatagtat aatataaaaa taccactatg tcaataaaat tttttagcat tcttaaataa 23340 cttgacttta atcaattttt gaaaaaattt tatttgccaa cctctactaa aaatagtata 23400 atcacgcacc cgtgtacgcg atataaaatg attatctagt accatacatc tcccaataaa 23460 agcaagcaaa ttttttattt aaatgcaata tcataggctt atttaaaatt gtagttgctt 23520 tctcccgtat atattggtat aatatatttg taagttgata aacaagatct ttttgttgat 23580 cctctgttag cgaagtacac tataggagtg ttatttatgg gaaaagcacg tcaaaaaaga 23640 gagaaccgca atggttcaag aaagcgtggc aacaagtatg agagtaacgt aattcaagca 23700 gatttctctg gtgattacgc caatccagtt gctaaatccc tagttggtaa aaaccgtgag 23760 caaaaatcat atatcaatat gatcaagaac aacacagtga ctgtgggtat tggtgaacca 23820 ggtactggta aaacctttat tccgtccgtt cttgcagctc aggaactcgt agacattcac 23880 tcagatattg agcaagtgat cctcgtgcgt ccgaacgaac ctctaggtaa atctcttggt 23940 atgctcccag gtgatctggc agaaaagcta gagccttggt tggaaccaat agccgatggc 24000 atgaaatggg caatcggtga tcacgcctac aaaggatatg tcgaacgtca gaaaattaaa 24060 ttcctggcta tcgaacatgc ccgtggccga actttcaaca actcctatgt aattgtcgat 24120 gaagctcaga atatctccgt tgaagcaatg atctgtctct tgactcgtgt cggacaagat 24180 tgtcgtctaa ttatctgtgg agatatagct cagaaagaca tcaagggtga ttcaggtcta 24240 gcacttctta tggaagtcta tgagaagtac gaaaactctc ctttctctat gatcgaactg 24300 atcgacaacg ttcgctcacc agagtctaaa gcattctacg atatctttaa ggatatgggg 24360 aaggtgtaat atgggaaacg tcgtacatct gcaccgcaaa actaagatcc accgtacttc 24420 attgagtgct gctaatatga ttacacgtaa ggaaggagaa gaaagtccta agactacctt 24480 agcgtggaaa attgtagcct ctaatccaaa tcggcctttc aactataatg aactgagtac 24540 ttctatagac attcttatgg ctaaggaaat acgacagaaa ctaactgata tgaccgataa 24600 acttaaatgg taagacagac gagcctgggt atgaaaatat ccgggctctt tttatctgta 24660 aaattgtact tgcaaaatga cccaaaacaa agtagaatat ttcctaaatc ctgataacta 24720 caaaaaggaa agccaaatga gccatcgcat cgaaaaagta attaaacgtg acggtactgt 24780 tcaggacttt gctcctgaaa aactcaatga ttgggctgca tgggcatgta aagatactga 24840 cgtaagttgg tctgccatag ttatggctgc cctgaaaaat ctacccaaag gtgttgtaga 24900 ctccgataca ctccaagatg tgttaattaa agccgctgaa ggtcttatca aagacaaccc 24960 agcatatgat actgtagcaa aggaattacg tcttgcgcag atgcgtaaac gcctatatga 25020 ttcctttgaa cctccttctc tgcgtttctt ccatgatcat atggttcgtg ttggcgcttg 25080 ggaagacatg agtgcatgga ttagcgatga tcagtttgat gctctgaatc aagttattga 25140 tcatgatcgc gatcgtttat tcaccagtgg tggactgaaa cagttcttcg acaagtattc 25200 ccgtcgtaat attgcaactg gtgaaattta tgaaactccg cagtttgcct acatgggtat 25260 ggcaatggcg atgttatctc aacctaactg gacaattcta gatgcaatcg acctctacaa 25320 tgcaatgtcg ctccacaaaa tcaacgttcc tacgccgcca ctggttggtc tgcgctctag 25380 tgaccgtgga tttgctagtt gctgcctcgt ggattccact gacacgctgg attcaatcga 25440 cacagccgag cacatcgtct tcaaaatggt cgcagccaga gcgggaatcg ggtatcatct 25500 tgaaagccga tcaattgctg atccggtgcg aaatggggca ttcccgcatt ccggaaaact 25560 gccatattat cgacacattg accgatcagt aaaagctaac actcagcaaa cccgtggcgg 25620 ttctgctact gtgtcttatc catacttcga tcctgaaatc attcagttga tgcaggttaa 25680 gcagcaacgt gctacagacg agaataaaat cgataagatg gattactctc tgagctttaa 25740 caaccttcta ttaaaacgtt acctgaaaaa cgaagatatt acgctaatgt cctacttcta 25800 cgcaccagaa gttcatgaag cgttttatgg cgaagatgag gctaagtttg aagaaatcta 25860 cgtggcagcg gagaaacgtg tggcttctct tacaaagatc gaccacgaag gaaaaacaat 25920 tccagcagct cctagaatct ctgcaaaaga aatcctagat acttggttac gtatccgtat 25980 ggaaacaggg cgtatgtatg ctcatcacat tggtgaatct aaccgtcatg gtaacttcct 26040 tgacccgatt cgtatgacca acctatgtgt tgaaattact cagccaacca ggccattcca 26100 tcacattacg gagctgtata agacccaaga tcaattagat gatatgcgtc cagaagatat 26160 tggtgaagta tctctgtgta acttaggtgg tgttgtactt ggacgcatgg aatctctggc 26220 tgagtgggaa aaaacttgct acatcctact gaaattcgtt gatacaatta ttgaaattca 26280 ggattatccg ttcccaacta tggaatatac ggctaagaaa cgtcgtaatg ttggtattgg 26340 cctgatgaac gcagcaggtg caatggcagc agaaggtctg gcttatgaag gtattgaagc 26400 ccgtaactgg attcaccgtg aggctgaaaa actgtcttac ttcctgcata aagcctctgt 26460 acgcctagct aaagaacagg gtgcatgtga gtggtttgat cgtactaaac catctaaagg 26520 aattctggta atcgacacgt acaagaaaac tgttgatgaa ctagtatccg taggtctaga 26580 aatggattgg gagtctctgc gtgcagatat tctgaaatat ggtatgcgta attctgttct 26640 gactgctcag atgccaggag aaagttcttc tgttctgcta ggagttacta actctatcga 26700 accgcctcgt aagattatat ctatcaaggg tagtgcagtg aataaagtaa ttgcaattgc 26760 tccaggtgca accgattggg aaacgttaat gagctataaa ctggcttatg atgtagatcg 26820 tatcgagtgg atcaaatggg tagcaactat gcagaaattc ttcagtcagt ctatcagtac 26880 gaatatgtac tatgactaca ccaagtttga taatgaaatc attcctggcc cagtagtagt 26940 tcgtgatttc atgactgctg ttaagtatgg gtggaaaact ttctactatg caaacttcaa 27000 taccgcaaat ggtggaggtg ctggagaaga agcagccggt tgtgcatccg gcggttgtac 27060 tatttaataa aaacaaggag atcttcgggt ctcctttttc tttgaaattt tacttgctaa 27120 tcgctaccag atttgctata atatctccat tagttaagga gattagtttt gataacacaa 27180 gatgaattaa aaaggattct ggagtataat ccagatgaag gaacatttac ttggttaaat 27240 aaccaagaag tacacaataa agtaagagga ctacctgctg gttgtttaaa accccactca 27300 gtagatccta accgaaaaga cttagtaata caaatcaatg gtaagaaata tcctgcacaa 27360 aatattgctt tcctgtacat gacaggaaac ttacctaaag ttatggttga tcatatagat 27420 ggagattcac taaactataa gtggactaat ctaagagaag cagactggca gttaaatagt 27480 caaaatagaa aaaccagtaa aaacaatata ttgggtacta agaatattcg gaagaaaggg 27540 tctggatttc aagttagaaa atccattaac ggaaaagaat acgtgaaaac tcttccaact 27600 ctcgaagagg caatagagta tcgagattct ttaatcctct ctataaccaa agacccaaca 27660 ctacttagaa acacagataa ataggagaac taatgacaac tttacttaac ttaaactggg 27720 atcataccaa taaagatcta tttctcggtg attctcttgg tattgcagat tatgtacgag 27780 tagctcatcc agagctagaa aggctggcgt tattagctcg ctctcaattc tggactgaaa 27840 ctgaaattag tcttgaagct gataaaaagc agtggggtaa tctcccgcgt gatattcagg 27900 aaattaccct cctgaactta gcatggcaaa cccaaacaga ttctttcatt agtcgtgccc 27960 ctgaagcagc tattatgcca cttgtaagcc gtcctgaact ggaaggtatg ctcaaacagt 28020 ggagctattt cgaagatctg catagccgtg cgtacagtaa cattatccgt aacgtgctga 28080 ctgatccagc agaatttatt gattctgtaa ccaaaaacca agaagcattc gcacgtatcg 28140 tggattctgt tgaattgttt gatgaattat atcagttagg tcagtatttc cttgcagttc 28200 gtgatcatca gggtgataat acttatccag aaactgaatt ccctgaagtt aaacgtgaaa 28260 ctcaggctaa actgctagat gcttactttg caatctatgg tctggaagca atgcagttct 28320 acgcatcttt tgcatgtacc tttgctctag cagagaatga cattctacaa ggtattgcta 28380 agaacctgca attaatcgcc aaagatgaag ctctacacac tcaaatgtct aaagctatca 28440 tccagattat gttccagcag tttgataaag atctggtaga tgaagcattg gctaaggcac 28500 cagcacaact cctgaaaact ctgaaaactg agattgaatg gggtcacttt atctttaaag 28560 gccgtagtct gattggtttg aacgcagaac tgctcgaaga atatctttac tttgtaggac 28620 gtaatgcatt tatgcacatc ggtgttgaat ggccaagcca cttacctgta atcacgaaga 28680 accctattcc atggattatg aactggttgg atactacgtc tttacagcct gctccacaag 28740 agattcagat tggtgcagca taccgtgtag gtcaagtaac tgaaacctct gctgatactc 28800 tgaaagatct tggcaacgaa tttgagggta tgttctaatg atagcagcaa tctatgcaac 28860 tgggccgaat ggggagttcg gcctcaacgg taaactccct tggggttctt tcaaagaaga 28920 actagatatc ttctacgcta ctttggattc tctagcacct gataatatta ttgtaggagc 28980 agggacttgg ttagccttcc ctgagactct gcaaaaaaga atactaggag attctgacct 29040 atttatttat gcaaatagac cactacctaa ctctgtagga tcagccacac gcataagtaa 29100 tattggatat agtctgcctg atttcctcaa ggaagaagta actgtagtat taggaggagc 29160 aactctgctt cttgaaatgt acgtcaataa tcatctagat atggcgtatg tatctactat 29220 taattcagtt acaccattat ctgctgatgt acatcttcac catcatatta ctatgactaa 29280 ttcaacagca actaggatgg actatgcagt agggcataat gcagacaaaa gtttatcttt 29340 tgtacaagaa ctggtgactt attaatgcgc caatatattg aaactggaaa acgtattatt 29400 gaagagggtg tatgggtaac gaatcctaga acaggggttc gttgcctaac cattattgat 29460 gcagacttta cctttaacgt aggagcaaga gaactgccgc tagtaactac tcgtaaagca 29520 tttgctaaac aagcaataat ggaaatgatc ggatatctcc gtgggtacga ctcagcagca 29580 caattccgcg ctattggtgt taatacatgg aatgctaatg caaatgaaaa tattgcctgg 29640 ctaggtaatc cattccgtaa aggcgaggat gatatgggac gagtctatgg tgttcaagga 29700 cgtcattgga ctaattctga tggtatggta tttgatcagt tagccaaagt ctatcataat 29760 ttgaaaaggg ggattgacga tcgtggagaa attttaactt tctggaaccc tggggaattt 29820 cgtaaaggat gcctacgtcc gtgtatgcat acccatcaat tctctatttt aggtgataca 29880 ctatacctaa actctttcca gaggtctaat gatttcttgc tgggccaagt atttaatatg 29940 gtacaatgct atgcacttct agctattatg gcacagatta caggtcttaa gccaggtatt 30000 gctcgtcaaa agattatcaa tcttcatatc tatgaagatc agtataatgt tctgatggag 30060 cacaagcaat tcgatcgtga accttttgca cctcctactt tagaaatcaa tccaaatatt 30120 agaactttgg atgatgtatt aacatgggta agtaaggatg attttactgt tcgtggctat 30180 gaacaccatc ctgctattgc atatccattt acggtgtaac atatgttaat tatactatta 30240 tttgtcatat tctttctaaa acattttatg gccgattatc cactccaaac accatatatg 30300 ctaagaaaga cgcataaaat agggtggaaa cttccattat taacccattc gctagtacat 30360 gctggattaa catttgcaat tctaatccta ttcacatcac caggaatgtc tatagcttta 30420 gctattatgg aattaattct acactttatt atagattatt ggaaagcaca acgctgtaaa 30480 tatgctataa gcgatgctaa gttttggata gctttaggat ttgatcagtt actgcattat 30540 ctgacatacg ccctaataat cttgattata atctaattca aatagccctt cggggctatt 30600 atatttaaat ttcatttgct tacttagtga aattcaaata taatatactt ctaatttgac 30660 agaaggattt aataatgaca gccatagtag catttaaata taaagataaa gtatacttag 30720 ctggtgatcg tatgggttct aatggctata cgaaactgct ggttaaaaat ccgaaagtat 30780 tcaaaatctc tgatgatttg tacttcgggt acactagttc cttctacatg ggacaattac 30840 tagagcatag ctttactgta ccaccacgtt tagcagatat ttccgatgat aaatatttat 30900 atacggaaat agtacctaaa ttacgtgata tgtttaacca gaaagatttt ggcgaaaaga 30960 aaaatgaaaa atatgctgaa cctaatctgg gacaatttat tatgctctac aaaggaagaa 31020 tctttatttt ccaatctaat gcttctattc tagaagttga atatgccggt ataggctgtg 31080 gaggagaaga tgtactttcc agtattcgta caggtttagc tattgcggat tgtcgagcca 31140 cagatttaga tgtagatgat atatttgaaa tcattgatct ggcttttacg cattgtgcag 31200 attatatgtg tggtgtatct tctcaacatg atgttttagt aattggagaa taaatatgtc 31260 tattctttta tctgctttag aagcaaaaca aaatactatt gctagcatta aaaaggaagt 31320 aattgaccta gaatcactta ttgccgtaga gattgacaaa gctattaaag ataaagaatt 31380 taactgtcac atccagggat taactgaata ctcagaaaac gctagaaata gcgtcatgca 31440 aaaacttagc aaactaggtt atgttgtaga agctaagtat gaacagcgtg aaggtttttg 31500 gcttgaaatt gattggagca aataatgtcg gttttccaca tatatacaga tggtgcatgt 31560 aagggcaatc ctggtttagg tgcgtgggga ttcgttgtct ataatgataa tgatgatcgt 31620 attggctcta aatctggctt cagccctaaa actactaata acgaaatgga acttactgca 31680 attgtagaag ttcttcgttg gtctactaag aaagataatc gtccagtaat tatctacact 31740 gattctgctt actgtaaaaa tggtatggaa agctggatgt ggtcttggca gaagaaaggc 31800 tggaagaagg gagatggtga aactcctctc aatctagaac tctggcaaga agcatttaaa 31860 ctaactcaac agtatattaa ctttcatgat accaatccaa cctttgttaa ggttaaaggt 31920 cactcaggta ttcgtggtaa tgaagaagtt gatgctctat gcaatactgt aattactgag 31980 caagaacttg ctgagatgta ataaataatt taaaaattca cttgcttaaa gccctcgaat 32040 ttagtataat attcgtattg aaagtgagga gggctttatg cgaatttaca actcaaattc 32100 attaggattt aaacctttga gaaaacgcgc acaatctccg cgtcagatca gaaaggcaaa 32160 tattggtgag ccagaaacta ggcttccacc acctccggag caacgtctag tttacctaga 32220 tgaagaatta gccgaaaggg aacgcaaagc tcaagaagaa attgagcgca agaaactgtg 32280 tactgctcct gcatataaca agggtggtta tcagtacgtt tccagtgagg aacaagctaa 32340 gatgatcggc agataatttt taaaaaattc atttgctaaa cgattaaaaa tttcgtataa 32400 tatatctcat aaattgatca acaaaaagga aaacaaataa tggctaagca gaaaaacaca 32460 aaaactcagg cagctccggc agttaaagct ttcactcaga ccgaagccaa tcgtaaggct 32520 cgtctggaac gtcatctgcg taagcatcct actgatgctc aggctcagac tgccctgaag 32580 tctccggctc cggttcgcca gaagccgaag gccaagaact ctactcgcgc aactgctaag 32640 caggttgtgt tcatcaaggg tgagggtcat aagtctgtac cggtaactct gagctttaac 32700 ggcggtgctg agatgttcgc ccgcaacggt atggctctga aagactacga aaaagcagtc 32760 aatcagaaac gtaagccaat ggctgacgta atgcgtgaat cccgtggaca gtttggctcc 32820 gttaaaccga atatcttcgg tgtagagtac agccgtgaca acgttcgcgc tctgtgctat 32880 ggtatcggta ttaagtttac cggtgcttct gatcgtaaat ccgctaaacc tgctcgtaag 32940 cgtaaagcga agtaatgatt gatggggcga aagccccata tactagctct ttctctgatt 33000 aatcaaggat attttatgtt ctctaagaaa ccacgttccg ttactgaaat cgttgcttcc 33060 tttaccacta ttactgatga acttcaagct cgtattgaag ctgaccagaa aactgctgct 33120 gatattcaga aacagcagga agaactcgct ctgaaactgg ctgaaaccaa taagagtgaa 33180 aaatctgctc aaactattaa ggaaaatatc cttaaactac tgggcaagta atttaaatca 33240 gggggcgaaa gcccctctta tttggagaaa ctatgcgtaa cttcgttgca aagaatgatt 33300 ttaaccgtgc tagcactcat aagtctgcta gagactacac tcgtctttct aaacatgagc 33360 ttctagaaga aatcatggaa gaaaactggg atgttaacga aaatggaaca gacctgtttg 33420 aagaagagga aattcctctg aaagacccca aacagtggga gtctattaaa gagacagctc 33480 tttaatagtt ttctacaaca ataaaggaca taattatgaa attcaagccc tttggtatgc 33540 tggacattga atctctaggt actccaggaa attgcggtac tacccatatc gcaatgcctt 33600 ctttcgcttt tgtagcgatg cacggaattg ataaagatcc tggtctggta ttcgttactc 33660 tagacgttca agaccagctt aatgcaggtg ctaaagttac agcgtctacc ctagctttct 33720 ggatggatca agctaaaaac agtccatctg cgctccatat tatggaggct ctaaatggtc 33780 gtaatcctaa actgattgcc ttccgtgatg gtaagcatca ctgcactaat gacgtaggct 33840 ctaattatgc tgccttctct atggctcaga acattatgga attgtctcta ggagataatg 33900 ctctgtatta cggcaatggc ccagagtttg atatgacaat ctactctgca aatacgttcc 33960 atgcaggaac ccatgaagaa atagttccgt ggaaattctg gaacttaggt aacgttcgat 34020 ctttccgtaa tcagtggatg caagctggtt atagctataa agatctagaa gccgaaggtg 34080 ctagctgggc aaaagctaaa atggaaaaga tggacaccat tcgttatgga atctatcctg 34140 tgaagcatga tccagcattc gatgctttag ttgaaagtta ttgtgtagcc atcatgatcg 34200 aaaaaatcaa aatttgattt gctttcagct ccaaaattct gtataatata ttcataaatt 34260 tgagagaaca acaaaaggaa aataattatg ccagtatcta aaaacgtccg taaaaatggt 34320 aaagtagcaa aacgtaacct gggtctgcgt cgtatggctg aacgtcagtc cggcatccag 34380 aaaatcttta acatactaaa tcgcgccaat ccgaatattg ataacagttc tgatactgtt 34440 ctgcgcactc tgctggcaat cggtctgtaa tagaccgttt ttaaaacaaa ttcaaatctc 34500 aaacttcata taaggaagca aataatgact actcgtatct ctaaaactcg tgctctggca 34560 actatcaagt ctctggaagc taaaatccgt aaagctactg aacaacagct tttgatcgct 34620 gttggtgaag gtaaggacaa gaaccaagtt gtagttggcg ctgcaatcga agtagatgct 34680 ctgtctactc gtattaagac tgatttccag tctctactgg atatgatgac ccagcgtgat 34740 catctgaaag cagcgctgat caagagcaat gctgaaactg ttgtagagat cggctctcgc 34800 tctatgactg ttgcggaagc aatcgaagct aagcgttcta tggaactcaa ggctcagttg 34860 ctggctaaca tgcgtaagca gttccatgct gctactgtta agtttaacac tcagaaggct 34920 cagtttgatg ccaagtatga gcgtttgcag gataccatgg ctacccgcga caagaagact 34980 tccgaggatg aagttaagat gcagcttaac ctgctggaac tcaagaacac tccgttcctg 35040 atcgatccgc tggagctgga aaagctgatc aagcaacacg acgaagaata ccaggacttt 35100 gcaactaacg ttgactttgt tctgtctgag tccaatgcct ctacttttat cgaagtagag 35160 taataattta aaatactagt aaatcggtag acgaaagccc aagcatctta cctcgcagca 35220 gtggttgaaa tacgaggtta aataagaccg ctgaccatac tagatacggt ataatctatt 35280 ggagattatc ttaatttatt tttcggcaga tgttgcatct gtcaaaataa aacaagacgt 35340 cgttggtagt accaacacaa cttcaaagat caacgctcaa gtatgaaagc tgaaaagtta 35400 aaagcactca aacttctaac cttctaaagc taaaagatgc aaagtattcg ttgatgattc 35460 aaaccctaga tcaaaggtac atggctcata agatatggcc tgtggcgcct ctaggctgtt 35520 tatcggttta ctagttctct caatctaaaa atgtatttgc aaaacagttt aattatctgt 35580 ataatatatt tttaaattga tgagacgagg ctattatgat ttttcatatt ctgattgatg 35640 atgtgcgtaa ccttcatgga atggacatta tcattcgaac ccccgaagcc gctgttgagt 35700 ttctgaataa aaccgatacc acaggtcatt tcgtttatat ggataacgat ctggctgtag 35760 atggtatgga aggttatcaa attcttcgtt tactactaga atttggtcag agacccaaga 35820 aagtggtact agtaacctcg aacccagtag ctaaagagac catgcgtaat gatttgcttg 35880 acttagggta taaagaaaat cccaatcgtg ttgaatatga ttggcaggag taaaaagaat 35940 gaaggcagct ttgcttttag tagctgccct taccttcagc tttagtagtc aggcatcgca 36000 cgatgcaaaa gaaatagatt gcattgctaa aaatatctat ttcgagagtc gtggtgaggg 36060 cataaaggga atgacggcga ttgcacatgt tactaaaaac cgtgtaaact ccggtaaatt 36120 ccccgattcc tactgtaaag tagtatatca gtctaaccag ttctcttggg tggccaggag 36180 accaaaagtt gataaaaccg atgaggcttg gcaagttgct aagaatttag ctagagtgat 36240 atactacgtt gatttaccac aagatccaac acatggagcg ttatacttcc atagtggtaa 36300 agataaacct tactggacaa agaaatttaa gaaaacaact aagattaagg gacatacatt 36360 ttataagcct gttgtcacta aaacttaaaa atttattaaa tgaaaaaatt aacacaagaa 36420 gaagtagaga gttacctagt tagtagaggc ttattcacta atgataagta tactaatact 36480 aggactaaaa tgcagttctg ttgcacctat ggacatgttt ttaatgctac cctaaataat 36540 attaggaacg gaagaggttg tccctactgc tatggaaatg ttaggtacac tcaagaacaa 36600 gtatctgaat atttgcgtag tttaggatat tacaccatta ccaaatatat aaataataac 36660 tctaagctac aacttacttg tagtaaaggg catgtatgga ttaccacttt tagtgccata 36720 aaaaatatgg gtaataggtg ccctgagtgc tccaatatat ggagcgatgg tgggatactt 36780 tatataataa ctagttcatt aggtacaaaa ataggaatta ctaattctcc tgctcgtagg 36840 ttaagggatt tgattactgc atccggggat cccacagcta agtattatgg ccactataca 36900 tatggtagct cttctaaatc agaaaccctt gctttagaga agaaggcaca tatgtatttt 36960 aaaagtcata attgtggtta tagtggattt gctggatcta ctgaatactt taatatttcc 37020 cccgaagagg cagaagaatt cctcttgaaa gagggggcca caatagtaca gaaaggggta 37080 gtttatgaag aacttgatcg tcattggtgt taatactcgt ggtatccgta ctacgaaaac 37140 agttaaaact actaacgttg ctgaaattac tacaaaccct aagaagtttg atttcgctaa 37200 agtctcggca gttatgacag aacaatcttt taaccaagca ctgggaatta aataatggaa 37260 gagaatattc tagatgtttt gcgtagggct ttgcaggaat ctaaggttga tggttctgat 37320 aaagaagtag ctaaaagcta ccagaccata atcggagatc ttcagcgtgt tgataaagac 37380 ttcattactt ctgaacagtt tgtatcttat ctaaaagctc aactgaaaag cattaatcag 37440 acgaaagcta aacttcacgg tcaagacctt gataattata gcttgcagtc tgctcagtat 37500 gaatatatac tgaacaaatg gttacaacag tatctgccgc ctcagctttc tgattctgag 37560 attcgcaaat actttgcaga attagttaag ttgaaccctg ggattacgaa gggtatgctg 37620 atgaaagcga ttaaggaaga attccctggt agttatgatg gtggtattgc tgctcaagtt 37680 gctggcgaat tcaataaaaa ttgatttgct aaacgcttca aaattctgta taatagattc 37740 atgaaaagga aagaataccc tactaaagaa atacttgaaa aatattttgt agctgatcct 37800 gataatggga tactatatag gaaaacaaag aaaggtttga aagagtgttg tgccaataat 37860 aagaaatcac atccagatgt gggatttcag ggcgacacct atcaactgca tgtcttatta 37920 tttattatgt actatggata taggcctgaa gaagtagatc ataaggatac taatcctaga 37980 aataatagga aaaataatct aagggacgcc tctagatcgc aaaataatgc gaatagaaac 38040 caacaatgca ataatacttc tgggtacaaa ggagtaagtt gggaaggctc ttcctggaga 38100 taccaagtaa gacatgaagg aaaaaattac agtaaaagag ggtttaaaac tgcggaagag 38160 gctttcgagg cctctaagat attaagagaa tccttacata aagaatttac taactttggt 38220 tagtaatagt ggggtgtcga aaccacttat gtaacaacac tgccgtcctg ggctgcctgg 38280 ataagccagg cattgagtcg atgtaggttc gagtcccacg gtcggaaaga gttggataac 38340 tggtgcttat accggataag cactcgctgc tggagtacat actatgacgg ttcaggaggt 38400 acgccgcctg atcagtatga ggttagaatc gaaacagcta aaccacgaaa ggactgggat 38460 gcttgctgag ctacccctac ctgatacatg cctcagctaa cttcgcatcg aaaatcggag 38520 gtgcgattta accagaccgc gtaactatga gagtgggaaa tggtaaactg tatagcttga 38580 ctctaactta gttaggtggc tacagagcct taatgggaca gtctagatac ttagtgaacc 38640 gcggataacg ctaagaatac tgtgaaaata ctatgggagt catgacccgg atgttgcccg 38700 atcgctatag tggcgaataa ccacgaagtc accttgactt aaactggaag atattgttcg 38760 acggaagggc tgaagagctt aatctgaggg ggcttaccct acctaccgcc gggtatcttt 38820 aagagttctt acgagagttc ttaaacatac ctaaacatta aggaatacta atgaatctag 38880 gaactacttg tatatttgat cacagagaaa tgtgttgccc acatatcctc tacgtagttc 38940 actttgcaga gcgtcttacg ctggatgaag ctcgtgagaa aatgagtgga attactggat 39000 atgacttccg tgaaggtgaa tactttagcg ttgctcttga ttacattgac aatacccgtc 39060 tttatcatct agtgcagcta ggggaagaaa ctgatgaagt gaaaatggcg aaagatactt 39120 tacgtcgtta tcgtcctcag attctagaac gtacacttcg tggtgagttc agtgacccat 39180 tctttttgaa gtgttatgat gcagacgcta atgagtactt ggggcgacaa aggttttacc 39240 aagaaatcaa agagtttgtg ggtgaaaaat acgaagcact actcgttgat tctagagcac 39300 gacgtgcagc taaaaatgct gagaaatcag ctaaagcagc actagaaagt caaaccaaac 39360 tgaacgaaat cgttaagtga tctaatttcg gggtgtagtc taagggatag gcaggagtct 39420 tctaaattcc tttatgcagg ttcgaatcct gtcacctcgg ccaattttat taactgctag 39480 gaaaataaag tgaaaacacg atatatagta aaagtagtag gtgaacattc acaagtaaat 39540 accaccgatg ttacagcact agcatttgaa gtagtctccg gtatagtcat atttcataat 39600 ggagatatca aagctcctga atttgctgtg gattccagcc tgtttatttc tgcaactaaa 39660 gtaactgtag tagctaactc ttctaaataa taaggaaata tatgtttact aaaaacacta 39720 aagaactgac ttctgatcaa aaagtaattc gtactattaa acgttggggt attggtgctg 39780 tagttggtct tggtgccctt attctggccc tcaattccta tacagttgtt caggacggta 39840 cggttaaaac tcaaactttc ctgggtaaag ttagtcctaa tcctgtattg ccggggttcc 39900 atattgtcaa cccattcgct tcttttgata cttttagtac gaaagatatt tctatgaaac 39960 tggataaact tcaggttcct agccaggata agtttaagtc tacagttgac attaccgtta 40020 tgttgcagtt tgatggtgct aaagctccta tgaaccgtgt taacgcaggt actcaggatc 40080 aagctctgaa caagtacgtt gaagagaaga tgctgtctac gattcgtgag tttggtaagt 40140 ctgtaccaaa agcacaagat ctgttcgacg ctaagattca ggctcaactg caaaccgcta 40200 ttcagcaaga agtagaagag tacgcgcgtc cttatggcta caccgttaag caagtattcc 40260 ttcaggatat tactctgcca ccggtaatca tggaacaggt gcaaaacacc aaagttcgtg 40320 aagaacaagt taacgctgca aaagctgaac tagctcgtgt tgaacaagaa gcacagcaga 40380 aagttaagca agctgaggct gatcgttctg ctcgtgaaaa tatggcagta gctaacgaac 40440 gtgacgcaga tgctaaactg tatgcagcta agaaagaagc tgaagctaac accgctctgc 40500 agcgtaccat cactccagag atgattcgct ggaaacagct ggaagttgat atgaaacgcg 40560 ctgaaaaata tcagggtggc gttcctcaga ctgttgtagg tactggttat gatggccaga 40620 tgattatgga tatgcgtaat aagtaatagt tacacgtaat cttcgaaaca agcccgcgtt 40680 tcctccgaga gcgcgggctt ttctgtattt aaggcaaaag aaaagcccat aagttattca 40740 actaaggtct ttagtattta attctttaga atcatatact attattgcag caacaacgtg 40800 ctacccttaa gtgccctgcg attttcctct aaaaagttaa aaaattcgat tgcttgaatg 40860 ctaaattctc tgtataatat atttataaat ttgagagagg aatcaaatat gattaagact 40920 ggtctagtaa ttggtaagtt cgcaccatta acacgtggtc atattaacct aataaacaca 40980 gcagcaacta tgtgtgaacg agttatagtt gtagttagtc atgataatcg ctggctggat 41040 aaacaaaaca cacgtgatca ggaagtctta cagttaaaga accgacttcg ttggttagaa 41100 caaacttacg cggatattga gcacatttcg gtagagtaca ttattgaaga tgatattcct 41160 gaatatccga atggttggcc ggaatatgct agaatcctta atgaaaatta tggacatttg 41220 cctgagatca ccattttctc tagcgaacta gactatgatg cagaatataa gaaatatctg 41280 ccgaagtttg gtcattatat tgtagattct gatcggactc gtgttccaat ctcagctaca 41340 atgattcgta atgatcttat gaagtattgg gagtttcttc ctagcgtagt tcgtaaagat 41400 tacgtgcgta aggttgtaat cattgggact gaaagctcgg gaaagactac tttaacaaaa 41460 taccttgcga agctatacaa cacttcgtgg gtagaagaat atggccgaac gtattgtgaa 41520 gttgatctag ttggtaacga aaatctactt accagtgaag attacagtaa aattgcgttc 41580 agacacaaag agctagagtt tgaagcagaa cgtactgcaa ataagatcat gtttgtggat 41640 acaaatgctt ttattactga gttctaccac agactttatg aacatagtaa gaaccctgtg 41700 gttacagcaa ttgcaaaaga agaacattac gatttagtaa ttgttctagc gcctactgtc 41760 aagtgggtag atgatggttt acgtattaac tccgatcgtt caattacttc tgaactgttc 41820 gagcagatga agaaagaatt tccaaatcag ttccctgaag ggcgtactgt ttacatcgac 41880 tctccggact acaaagttcg tatggacgaa gcagtaaacg cagtagactg gctgattcat 41940 cactttaacg ttggagagtt ttaatatgac taactatctc gtaaatgact ttaaaaactg 42000 gaaacctttg gaatatctgt ggctgtttat agcggttgtc ttcattagtt tagcaaacta 42060 taatggtaca gctctagatt gggctactgc aataaccaat gtggtttgtg ttattctggt 42120 tgcaaaagga cgtgtttcca actatatttg gggcttagtg ggtgttctta catatggtat 42180 tacagcttta aacgcagatc tttatggtaa cgctgcacta aacctgctat actacgttcc 42240 tatgcagttt tatggaatgt acttatggca taaaaataag ccaagtaatc tgccagatgt 42300 tccaactaag gttctgacta aagcccaagc tatgctgact ctagggattc tagcagtagc 42360 cacgttaggg tatggtagtt tcttagctat tacggctgat ccatttccaa tgttggatgc 42420 atttactgct gtaggtagtg tgatcgctat gtggcttatg gtcaaacaat atgctgaaca 42480 atggctgatt tggattgcta ttaacattgg tactgtgtac atttggcttg taactgctca 42540 atctacaggt gcatcttacg caatcctagc aatgtggcta gtattcttag gcaactctat 42600 tcttggagct tataaaggat actttgtacg cgttaacaaa taaagtagtt atagccctct 42660 aagcgagggc tactatacag aaattttcac ttgcttaata tagaaaattt ctgtataata 42720 tatttataaa ttcgagagag gaatcaaaca tgaaacgcat tgtacttgta ttgactctag 42780 tatctacttc tgtatttgct gctccacaac aggaactttg tcaagttcaa gtttgtaata 42840 agctagaacg atttactcta gacatttggt cccacattaa agataatatg ggtgaacagt 42900 gttttgatat tgttctgcca aaagaacaag ctgttcctgg ggctgttctc agctcagaat 42960 cacgttggtg gcaaggttct tctatcaacc caactaagaa gtcagtgaca cgtatcaaac 43020 aagtatatca gtgcaacgtt cagtaaaatt tcacttgctt aatgccgaaa ttttctgtat 43080 aatatattca taaatttgga agattaggaa ctatttatgt tagactatga gtctatactt 43140 tatagattcc gtttagaaga tggtgtacta gtgtggaatt ccctctctgg aaactctaga 43200 gaggagaaga tatttaatac taagtttgcc aataaacctg cgggagcttt acacaaaagc 43260 tctggatata tcagagttaa cgttgatcac caagattact tagcccatat catagtattt 43320 atgttgtatt atggatatag accagaaatg gttgatcata aagactgcaa taagttaaat 43380 atccatcctc taaatttaag agcagcagat agtaccacta atacgcataa cgctagtaaa 43440 tcttcaagaa atactagcgg atttaaggga gtatcctacc acaaagcatc tggtcagtgg 43500 caaggtagaa taatgtttaa aggtaaatca tattccgcag ggctgcacag tactcctgaa 43560 gcagcaaatg aagctctaat tcaactgaga aagaaacttc atggagaata tgcccgaaat 43620 aattaaggaa gaatagcata acggtattgc agcagattgc taatctgtcg gtttgaaata 43680 tagccttgtg ggttcgatcc ccacttcttc ctccatattt ggttctcaag ctcatttggt 43740 atgagccgtc gcctcataag cgaaaggtag gtaggttcga atcctccggg agccaccaat 43800 taactaggag actatatgtc tagattctat gaaatgaaga aagcattaga agaatgtcgg 43860 tgtaaggatt gtcatggtgg tggtaggcag gatgatgcag aacctgggga tattttctgt 43920 agagaatggg aatgtcctac atgtaaaggt acgggtatca accctgaata tgcaatttgt 43980 ctagacattg agcgaggcta agatggatat ttttactact ccggcaataa accttttagg 44040 tgttggcttg ttccaagcta cggtgtatcg tactgatgat tctactgatg tggtaacgtt 44100 catcgtacct gagttcttcc tagaaaagtt ctttgaagag tttgagcagt tccgtgaaga 44160 acacaatgct cattccgata tggaagatct agcagctatg ttccctgcgg tttacggcta 44220 tatctttgaa ggtaatggcc tagatcttaa taaatctgaa ctcgttgaac ttaactgggg 44280 catcaatttt gaagttggct ctccattccc tcgatatttc cagggtttag agattcgcta 44340 agattacatg ggggtgtaat cgaattggca taggtactgg acttaaaatt caggttttgt 44400 gggttcgaat cccaccaccc ctaccaaatt aaggaaataa aatgaaagct attaagaaag 44460 cactacgtaa atttatgtac tgtcgttttc atagcatcaa ggcttcaact aatggtacta 44520 tttacgatcg ctatgatcct aagtttggct tacattctaa ataactggag aaaacaaatg 44580 gctaatcctg aaattacggc acaacgcttt atgctgaaag gaattattgc tgaagcaggc 44640 atgacagaag aagttgcaga ttttcaaaag caattcctca atattctcac tcaagccaaa 44700 gagctaggag agaaagaaca tggcgcggct attatggcaa tcacattggt tgggcttgat 44760 ctggctgaag aatcaggagt ttaactcctc caagcagaaa ttttaaaaat gtacttgctt 44820 tttacttaag atttctgcta taatagttat ataagttgat gagaggaatc agaatgttaa 44880 aacgttttgc agcaactggt attgaaagct cagtatgtaa atcctggggt ggttgggaag 44940 gtgagatcga gtggctttat ttctacaatg tagaacttct tccagagatc aaagctcaat 45000 gtattgaggc tggtatggct cctgatgcta cggctgatgt tgaaatcagc atgaataagc 45060 tagagggtcg agtagtcact gttactgatg aaggtgaaga ggtattcgaa cttccattca 45120 ctttgaaagt cactccttca tttgattaac tgatacagca ctgagcaagt tctttaaaat 45180 ttgacttgct caagtgcctt aaaacttgat ataatagtta tctaatttgg aaaacatatc 45240 tgaatgagaa gtaactaagc gagttgcttt taggggtaaa gtcaggaaat cctgaaatat 45300 tccaccttga gtttaaccga cttacttttc attcaaatat gttggattag tatcgtagag 45360 gtagcgaagc agactgtaaa tctgccgact cggaagggtc tcgggtggtt cgactccatc 45420 atccaacacc aaatttaagt cctgttagac aaactggtaa agtcactacc ctttcaaggt 45480 aggatttgcg ggttcgatcc ccgcacagga cgccaatttc tgcattccta gagtgttact 45540 ggacagcatg tcggtctcca aaaccgtacg gtctaggttc gagtcctagt aggtttgcca 45600 atatttgcac ctttagctga gatggattag cgcttgcctg aagagcttga gaggttcgtt 45660 caattcgaac agggtgcacc aatttgaagg agatgctgat gcatcctgaa aacttcttta 45720 tttactgtgc ggataagtca ctgaaacgca gaattaacaa agtctatcat tatcgtgcca 45780 gtcaacagcc ttacgtttac gcttttgacg gagatcacat tccactagaa cagttggcct 45840 gtgattacat ggtattgaag aactttgacg agacaaagat ctcaagcgtt cataatcgac 45900 agaacccgtt aacgtggaag tataagaaga aaaagaaagg catcatattt ctgaaaaatg 45960 tagttgctta accgcttaaa gttctgtata attattacat aaattgaact ggagaagcaa 46020 catgacagaa caagaactga aagaacgtaa gctggtaatg cttcgcttac ttaacatttt 46080 ctctggtatg gatggtggta ttgacttcat tcaactacgc actcttgtag aagaagctga 46140 aggcgaatct attactaaaa tccttaaaca tatgggttta ttactagacg ctgctaatgt 46200 agctactgaa tataaattag aagatctacc ggagcgtgtt tcatgaaaat gttagttaaa 46260 tttaagtgtt gtggttctga gcatacattc actcgttatc gggagtttaa gatgtgtgag 46320 tgtggtaaat caggctacga tgctggagat ggttattatg ctagatctat agggcattcc 46380 aaagacctag aatttactaa tctcgaaaaa caagataaat cgaccgttgg ctgaatggct 46440 taggcgaagg attgcaaatc cttttttatg tgagttcaaa tctcatgcgg tcgtccaatt 46500 tgctgaaaga attgttaaat caattcaggc acgcgtagga acactgcctc agtaattgaa 46560 agatgaggca cggtttggag aggtatgccg ctacacgcta cggtgctaac tccgtctagg 46620 tgaaaatcct agtcatcaat ataaatttag ggtcgttggc tgaacggttt aagcgccgga 46680 ctgttaatcc gtatccgcaa ggataaagta ggttcaactc ctacacggcc cgccaatttc 46740 actaacaact taggaaatag atatgacaac tcgtgcaatt gctcgactag aagctgatcg 46800 tgatagtctg attaataata ttaaggtttg ggatcaagaa attgaaaacc aaaaactgac 46860 attaattcaa gatatgcaac aacagaatgc ccgtcgtaaa gctctaaaag aggttgagga 46920 agctatcgaa aaactcaagg ctgaataaat tatgcacgga atatccttac ccattccagg 46980 gtttgaggag tacttagtaa gtttggatgg gttagtattt agtatcaaat caaacaaaat 47040 actaacccca ataaccagta attacggata cttatctata cgtctccata aaaatggtca 47100 acagagaaat accggtgtac ataggatttt agcatgtgca tttaagaaat taccaagtct 47160 agactctgaa ctagaagttg accatattaa cacagacatt acttattggg gtcttaataa 47220 cttacaagtg ctcacaaaag agcagcatct agaaaagact ttaaaagaca aaggatttag 47280 taaactaaac catgataatt gttgtaagga ttgtggtaaa gctatttctt taaaagctga 47340 aaaatgtcag gagtgctctt cacataaacc aaaagatatc tctataacta aagaaataat 47400 cgaggttaca gtaagagagc taggctggtc aggtgcaggg agaaagttaa gttattcaga 47460 taatgggttg cgtaaaagat acacttcttt aggaagcaat cctaaagaac ttaaaaagtt 47520 aaaggtacta tagtctaacg gttaggatac tcccctgtca cgggagaggt agggtttcga 47580 ttacccttag taccgccaaa ttatacatcc atagctcaat ggttagagct accgcctctt 47640 aagcggaggg ttctaggttc aagtcctagt gggtgtacca aatttaaaga gtaaaggcta 47700 cagtaggtgc acccttacgg attgctacac cggactcttt atcaaacatt ctagcgtggt 47760 ggagagggga caccagcttg ccgagggtag aagctcgttc gattcgagtg aaagaatggt 47820 tctcgtaaat ccgcacaagt gatagcaggt tcaagtcctg cctagattga cttataagta 47880 agcgaaagtt tacttattga gtaggttcca atagatcggc gggtctattc tggcatttaa 47940 gagaagtctc gcaaacttct ttgtcagccg tggggttcat agcctccagg tggcctacgc 48000 aataagtaaa tttattaaaa actcatttgc tattatactt aaatgtctgt ataatatatt 48060 ttataaagtg agttaacaaa gaatattctc cgattagctc aattggctag agtacaccgt 48120 ttggggcggt ggggttgaag gttcgagtcc ttcattggag accaaataat tgcgggttag 48180 atctctggta gagatcgcta gtctcataag ctagaaagag gtaggttcga ttcctgcacc 48240 cgcttccaat ttaatagaag gatttcgaaa tgaaattatt tcgtttaaag gtaagtgatt 48300 tacgttctag caaagatcgt aagaagtatc cactagcgca ctacggtaaa gatgaaaacg 48360 aagtgatggc ggaatttggt attaatcatc cttacttgaa agtctactct attaaggaag 48420 tttatgctta ttaacagtgt acttcaagcc ctggatcttc caaagcactt tactattact 48480 gactccttaa tacgtaggta tatggagaaa tataacgaaa tatctggtaa agaaggtaaa 48540 ctaaaatcct cagagtttaa gttctgctat agaatggcag cgttaaacct gctgaggtat 48600 aaaagggagc ataaaatcac tataacagaa gggtttgtat acgtaatagg taacccagca 48660 tggcctggac tctataagat aggtatgaca gtagacgtaa atgaaagact gtctagttat 48720 caaacatatt cgccctatcg tgattacaag ttgtaccatt ttagattctc ctcggacaag 48780 agaagaactg aaaaagcatt acataatctt ctgaaagact acaaagtatc aggagaatgg 48840 tttgaagtac ctctgttaga cgctttcaaa ctattagtag agaatttgga ttgctagctc 48900 aactggataa gagtagcgga cctttaatcc gtcagttctg ggttcgaatc ccaggcaatc 48960 caccaaattt ttgctcggtt agtttaatgg gagaaccccg tctttacacg gcggttgcga 49020 tagttcgatt ctatcaccga gtaccaatta actcaaaata aggaattgca aatgcaatta 49080 tcaagcaagg ttcaaggcta tttcggtcat gtaacaatgg agttcgctat ctctaaaggt 49140 tgggatttcg agcaagatct agctgagttc tacaaagata ttgatctgaa cgtaccatac 49200 atgggactct ccactggtca agagatcaca gaaggcgatg tgttcaaggc aagtcaaggt 49260 gaggtaacac ctagttttgc caagttctgg ttaggactta ctttgaagga agatgcacca 49320 gaggatggtg tggttatgca gagtatgcac tctggaagct ctgattacct tttcgttagt 49380 gacgttaagg aaatacaaga aatctgcaaa aatgtacttg cttgatgctt aatttcttgt 49440 tataatattt atataaattg atgaggagaa atcctcatca aagttcacta aggtattagc 49500 tcgacctagg actggctgcc aaaaagcacg gttgcaatta gacccctaat ggtgggggat 49560 actttgcaag aagttctaga ttagtccact agattcagcg gcaactgaat agtgtatcgg 49620 gtttgtgaag agcggttatc ctctactggc taatgtctga gggtaatacg cacaccgagc 49680 taatagcctt agtaaactta aagattttgg ggaatgggtc tgcttggagt ggacacctcg 49740 cttgcaccga ggatatcaga agagttcgaa tctcttattc tccaccaaat tcggggtcat 49800 aggttatttg gttaaactta ctgccttgca agcagtggaa ctcagttcaa ttctgagtga 49860 ctccaccaaa caaattaagg aaataatatg gtattcttag cagcctctgt agttcttgca 49920 gctttagcac tagctatcta ttttagaact attaaagatt tagctaaaga aaagaaaact 49980 ttctttactg attattcttg gggcgctcca tatactgtcc gctttatcag cttagaagct 50040 atgctgataa tggtgttcag tggcgtaggt tccattgttg cgggattatt atttatttct 50100 aactaaataa aatggggatg tggcgaaatt ggcagacgcg ctagatttag attctagtct 50160 tcgggtgtgg gttcgagtcc ctccatccct accaaacatt ggattaactc atgatgaaaa 50220 ctcctataac tgatatctgg ttagctgtta atacattaac taaagagtat gtgtttatag 50280 agtatggtca gaggtatgac caaaagatgt ataatctcat acttccagta tccgctaatg 50340 aagtttatga tttaatcctg agatctacgg aagagcaaat cgattaggtg acgaaaaccg 50400 cttggaaagt ggctgactgg taaaacggct tgagagttcg aatctctctt cttccgccaa 50460 acaactggaa ggtaggacat agtggtatgt aacaggtctt gaaaacctgc ccgctgtagc 50520 gatatggtga tggttcgact ccattacctt ctgccaaatc ttgaagtatg ccggaatcgg 50580 tagacggggc ttcctaaagg gcgtaagggg aacatccact cctcataaga tacccgaaaa 50640 cgaggctgag ataaatggtc tatctcatga aggttcaagt ccttctgctt cttgatattc 50700 aatggctcaa agtgtaacgg tgctatttga tgttcttagt aagactttcc cgacatgcgt 50760 tcgggagtaa gctgctaagg ctagagtaga agagtttcgg agagccgcca caattgagta 50820 ttactagaga gcattcgcaa gaaagaatca ggagaaaaac ctgtgcgcag aatgactaac 50880 tttggtacct tagacggcca aatggaggac tgatcatccc ctaatagtat tcaattgtgg 50940 ctatatcata attggttaat gatcctgatt gtgaatcagg cctatgtgga ttcgaattcc 51000 actagccacc ccaaatattg cgtccttatt tcaacggaaa gaatgtaaag ctacgaactt 51060 tacgatcggg gttcgattcc ctgaggatgc accaattaac ttaaacagga aataaaatat 51120 ggctgatttc tgcaaagaat gctctattga tatgtgggga agagatactg gtgatctttc 51180 tggtctgatt acaaaagccg aagttaaaga aggctatggt gctgttgtaa tctgtgaagg 51240 ttgtggcatt atccgtgttg atcataatgg taaacgtcta gaagagcctg atccaatcgc 51300 cacggttcct gtagaataaa aaattattgg agtgtagctc aactggcaga gcgacggttt 51360 ctgacaccgt aggtttctag ttcgatccta gacattccag ccaaacaatt ggggattagc 51420 ttagcttggc ctaaagcttc ggcctttgaa gtcgagatca ttggttcaaa tccaatatcc 51480 cctgccaaac aataagcggt taagcacacg ggttgtgccg caggccttcc aagccccgct 51540 gagtagggtt cgattccctc tagccgctcc aactttaagg aaacatttat gcacgttttt 51600 gagtttcgtg ataaaagtaa caataaacga gttattgtta ttggagaaac atacctagca 51660 tacgaacaaa ctgacggctc tgttatcttg aatgatgaga aagcaaattt tagcttttat 51720 ccagaagaac cttatgaaga gtttcgtact aagtttcttt taccagactg gctagcagta 51780 gccgagcaac tagaatccga agatggctga taacctgaaa ctaaaggttg acaaacaaaa 51840 gaaacgtgta gagattgagc ttggtgacga acgcctagca ctatccttcg agtttcttca 51900 gcagttggcg atgacagtaa tgtctgataa gtctcctgta tttgaaaaag aaatcccgtt 51960 gaaatgacgg gtttattgct cctaaagcat tgctggcgat gcagttgcct tgtaagcatc 52020 tgaactgggt tcgactcctg gtgggagcac caaatttaaa aattcatttg ccttctctct 52080 tatttcttgt tataatattt atataaatta atgagaggga aaccaaatga aacgttatct 52140 ttctgtcgtg ttccaaactg gtggtcaacg ctacacttat gaatttcctg agtcctggaa 52200 aattaaagaa ggtgatcaag ttgttgtatt aacacctcgt gaaggcttca aagtagtaac 52260 ggttaaacaa gtctttccaa aagatcacga accatctaag tctattcagt acaagatgat 52320 tcacggtctg gtacgaaaag tacctagaac agaagtcgaa gtagataaaa ctggggaagc 52380 taaataccag tatacttctt acttggatga aatgatgtaa tgctctgata gttcaacagg 52440 ttagaacagg cgaccgataa tcgtcaaatc ttggttcgat cccaagtcgg agtaccaaat 52500 tatagttagt tggcagagtg gttatgcacc tccttcatac ggagcgacta cagtggttca 52560 actccactac taactaccaa agataagatc aggcctttgt gccagatctc tgtgtttatt 52620 aaactgaatg ggactatcaa tccacaagcc ggactacgag cgattatcgg gtattcactt 52680 aataaacagc ctgcctccca gtttttatct tgcttctggg ttaacaaaaa gctgagccaa 52740 attctaacat cagggagatc taatgaaagc atatcaaagt cttaagtctg gcgtaattaa 52800 cattgttcat aacaatcaga tcattcaact atattctaaa gatggtgaac ttaaacagaa 52860 agttctagtc gaagatctag aaggtatcac acctcacttt gaccctgagg cctttcgcat 52920 tgtagatgtg gaagtggttc ctcaagtcga gggtggtcag cacctaaatg tcaatgtgtt 52980 aagccgtgat cagcttttgg atgcacagaa acatcctgaa aaatatcctc agttgaccat 53040 ccgtgtctct ggctatgctg ttcgatttaa cgcattgacg cgtgaacaac agaacgacgt 53100 tattagtcgt acatttactc aggcgatata atggggtttg gatacgattt ctcccagcta 53160 cgcaaggtag tcgagcaaac tccatttatc tcccaagttt taggagaaat gtgtggatcg 53220 agcattaaga cgtcatcacc gacaacgtgt gaagaacaac cgcaagaagt actggacagt 53280 ctttccacat gaagaaagcc ctaagcggtt aggtattatc actactactc cttgcatctg 53340 ttcttgttgg atgtgtggga accctcgcaa gtactataag aatagtaaag caggcatgaa 53400 aatctcggag attaaaaaga tggatgcaat gattatgggc atcaccttta caggtggtat 53460 ctctgatgaa ttcgacggtt ttgtaactca ggatattgtc gaaattatct aattaataat 53520 aaaccccagc tattgaataa gtagtctggg gttttcgtgt ataaggattc tcgaaaatga 53580 aaatttttag agtaaattac tcaggactgt ggtggttagg tggtacagct attgttgttg 53640 cggaaactgc ggaagaagca atcgaactgg ctagaaatca tagaagttct atagaattcg 53700 agaatgtgac agtagaagaa ctccctagca gtggggtagt atacaacaac aacggagatt 53760 actaatgtta tattttgaag ttcgcccaca ttaagttaat tcaaaagact taatggaggt 53820 aatatgagcc gtacttatcg tagacaatct ggtgatcagt ggtggaagtc taaagaacaa 53880 ttctctcaag aatattcttt catcaaagaa aatggctact ggattcgtgt tatttatgct 53940 aaatcctggg ataaggtaga acaagaaatg gaagaagcaa ccattcgtaa tgagaaaaag 54000 gatggttata actggaattc cattagtaag aatgttaagt ggcattctaa taaaatggtt 54060 cgtcaaggta atcgtcaaga actccaccgc gtgatgaaag atccagaaaa ttacgactat 54120 aatcgcgatc atgacatgcg taagcgtggg ttatggtggt gctacgacta atttctgaaa 54180 taattcattt gctaaacctc tcttttcttg ttataatatt tatataaatt gatgagagag 54240 gtttgaaatg aaaaatcgcc tagactgtgc tgttcacgta cttgagcatg atgttgttgg 54300 gacttgtatg tcctatcatg aagtaggcag aaggattgtt aaacgtgttg ctgatgtttg 54360 tagtttgaaa atcacagaag atgaagcaga cgaactgctt gagctagcaa ttgagaaaac 54420 tgagattttc ttatgcgatt cctgctcttg gtggtgtgaa gcccatgaac attcctacaa 54480 cgattatgat gtatgccgtg actgtaatga ggaagaccat gatgaataag tttattattg 54540 cactgatgat ctccgcagtt agtttcggtt ctctggcatc taccaaagtt tctatgaaaa 54600 atgggaatat tagggtacaa cagaacggca tcattactga atatggtaaa gtcagattag 54660 taaaggagcg taatggtaat atagaaatta tgaccaataa aaacttctct actccagcta 54720 ttaccattca gcgtaacggt aatattacta cccaacgttc caacaattct gactcgttta 54780 cttgtcgtta tgattgtgct gcggaagtag aagaatgatt accttaccac tttgggatgt 54840 aatggaaaag tttggtaaag atttgtgccc aggttgtata ccattgtttg ttactattct 54900 aattgacgaa aaaggatctt aaatgattat ctctccattt gctttgtggt ttgttattgg 54960 tgcagttgta gcgatttata gtctgattga tgatctgtat atctctaaaa ataaagatgt 55020 tattctttat gttataaaca aatatcgtca gccagaatta gcagaatttg acgatgaaaa 55080 gatgttcaaa ttcatcatgt caggtatgct tattatggac accttgttcg ggccagtagc 55140 tacttatttc ttcttccgta aaactcgtaa gatgaaaaaa ttccgtgctg aaatgcagag 55200 caagtaaaga actcgcgtag gaccgagttg cgtcacctgc aactgttacg gataagggag 55260 tcgtgcccct tagtgcggta agtggtgagt tggtcgcgga cctcaacttt aactggagat 55320 atcatgagct ttattttatt cattgtagca gcatggttta ttattggttt aggctatgct 55380 attgcagtta ttcgtcatct ggatgaatat tctgctgaat ggtttattaa gtacctacgt 55440 ttagacgatc aagaatacga gttcgacagt gatgaggaaa ggcaggctgt ccagtcaatg 55500 actcccaaag aatgcttact atacatgcgt gtaattggtt tctttggact gttggtagca 55560 ggaccgctag gtcttactat tgctccttac aaagaagtta ttgacgacat taaactatgg 55620 agaaccgctg gatcgctacg tcgtgctaga gaattaagta aacaagagtg atgataatca 55680 cctcctagca agaataataa tggcaaagcc cagtcgcaaa attgcaggct gggctatttt 55740 ttgtaataaa atttcagttg ctcagatgcc acaagtatta tataatatgt ttatagattg 55800 aggcaaaagg ataatttatg agaatcattt caaagttcaa tgatgtatac gatttgcaga 55860 atagtttatt tgatccagat cgtgtctggg agcgtaaaac tgaagagctg ctagtaaaag 55920 taaccgacga tgtagaaaag aatattgttc ataatcgtca ggtgtttcgt gaaggttcct 55980 tatccttccg tggtgatttc gaatactcag taaatccact gtttattgca ggtgaagttt 56040 actggttaca tgaactgtcc tgctggcatc cagcattcag tttcaaaacg ttcaacatcg 56100 atgctgtgtt cgataaaatg gaagaaatgg gattacatgc ccgttcttac ttactagata 56160 agaatcgtgg tgatgttcgt gaaaccatgc gagatatgtt ggttgaagca aaacctaaag 56220 cagaacgtat cttatctgaa ctgcgcgtac caattgcata tgtgaaaggt attaagaaga 56280 acgataacga tgatgttcgt aacttcgtaa ttcagacaaa cattcgtttc catcagtcag 56340 gtattccgtg gcaggaaatt gaaagtaact tataccgttt acaccaagtt ctcgaacagt 56400 atatcttcgg tgtactagga actggtgagc ctgatatgat taccgtatca gataaagaca 56460 ggttagcagc acatggattt gatacaaaaa cttctttcag gaatatggca aggtaagtgg 56520 tatatcctgg gagctggtct tgcgataggt atcggattcg gtgcttatca tttagtaaat 56580 aaggttgaaa ctttagcggg agatcttgca gttgcgacta aaaagatctc cactctagag 56640 acttctctta ataaagtgaa aactgagagt gaacttcgtg agactcgaat gaaccagtat 56700 ttcacaatga ataatatttc gcaagcagat ctagacaaaa agattaagca gttagataaa 56760 gctcttagtc gtcaagatgt tatcgctgca aagcctggat tggtaacatt aattgctaaa 56820 aagcagaata aagagttcga ggaaagatta gcgtgtttaa ctggaaactt ggagtactgc 56880 tcgcagccgc aatcacaatc acaggctgtg cagaagaaat aaaaccggag ccatcccatg 56940 agttaaagca agctcatgta gattggccta aagtacttca gccgtgtagc tttgatttca 57000 aattcgaaaa gaaattagct acaaatggtg aagatggcgt agtagttgtt gtaccttaca 57060 aagattggaa tgcaaaagca aaatgcgagg aagcagttta ctcttacatt tcacgactga 57120 caggtatggt ttgcttctat cgtcaagatt tacaagaaaa acgttgctta gtttattatc 57180 cacccattac aaataggaaa gattaatgtc agtattagtc ggtttacatg gtgaggctgg 57240 tgcgggaaaa gataccgttg cagaattaat gattgactgg tgtaatgaca cgttcccaac 57300 gtgtttattc cgtcgttata gttttgctaa acccgtttac gaacttgcat ccgtaatcct 57360 tggggtaact ccagagtttc tgggagagcg caggggaaaa gagattgacc aatggtttac 57420 agtcacacaa tctcaattgg agcgagctag agacgtatgg tttaagtatg gtatcgataa 57480 gtttgaggac ttctcgtacg tttggccgat atttgaagga aaataccttg atcctcagca 57540 gttaattggg caagctgatg atgcgctgta tagcgtattt atctccccaa gaaaaatgct 57600 acagcttgta ggtactgagt taggaaggca gctggtgcat gaacgcattt ggctcataat 57660 tctggagcaa tccatcgata aagacgaccc agacgtcgca atcgttaccg atgtgcgttt 57720 ccccaatgag ggagcgctaa tcatggaaac caattctcta gatatggatt ccatgcttat 57780 taaggttgaa tctggtatta ctaataagta tcgcatcgtg acagatcatc cgtctgaaag 57840 cggtattcct gaaaaataca tttctcataa atttgagaat accttcaacg gacttgatgc 57900 acttcgtacc gatgtgtacc acttctgtga cttagagcta gaaccactag ttggataatt 57960 aaggatcact atgactaata agaaagaaga aaagactaac ctgtttcaca ctgttcgcca 58020 atctaacgaa tatacctttt tctttgatga agaattaggc tcaccggatg agtatcgtga 58080 cctatccatg atactgatgc aggctggtga agatgatgaa attaatctga tgattaatgg 58140 gcctggtggt tacgttgata ctgctgcaca actatctaac ctaatcgcta actgtcgtgg 58200 aacggttatt ggacacctgc tcggtcctag tgcttctgct tactgcacaa tcttcctgtc 58260 ttgccatggt tgggttgtac atccacatgc tacgcttatg ggacatacgt tctctggcgg 58320 attctgtgag aaaggtcaag aaatcaagaa agcctatgaa tcctacaacc agtttgtaga 58380 agatatgatg ctagatattt actttccgtt cttctctgtc gacgaaatcg aggatatggt 58440 aaaagagaat aagaatatct atctcaacag caaagaaatc aataagcgta ttgaaatttt 58500 ggcgaattat cgtaaagagc agtgtagtaa agctcaagca ccgcacccag aagaacatgg 58560 agaagtgtaa gtagttttaa gccagagttt aacgactctg gcttttcttt tgcctaaaat 58620 tcttcttgac aacgacaaaa aattgtgctc cagaagtgat cttaaaaaac acattgtaat 58680 tttactctag aattagtata atggtagtgg ttagaggagg ttacaagtgg aaaagtttat 58740 acaactatta acgatactgc tacaagaagc gaaagatcca gcttcactac taaaacgtct 58800 gctaactatc ttagttgctg tcattatttt cttgtttgta agtaacacta gcgaggtgat 58860 gtcattttta aagactttct ccacgtcttc ggttttacag gatgttcaaa cccaaaggat 58920 agataacttt cctaatgtag cacgagaaaa gagtatggtt cttttctccc agactggtgc 58980 ggatgccgtt tttgtagtaa agtataaacc agatgctata aatgattatt caaacattat 59040 tgcatgggaa agcaatgcgc aattggatag ggctgacttg gccgataaag cagtaaataa 59100 gacgtctgaa ctttatagac gtcacctgga aggctttaac tacgctgcgg atttaagtgt 59160 aaaagtaaat aaatatacgg ggtcaaatat accagcgttt aagaacatta cttttaatta 59220 cgtctacact tgtccatatt tcaacctaaa taatatctat gctggataca taggtattgc 59280 atggaaggat aaacccgtag atacagctga ttcggaacaa tttaacgagt acttagcaaa 59340 gctctgttcc ccacaacgga gatcattagg tagatctata tgagttttaa atttggtaaa 59400 aatagcgaaa aacaattagc aactgttaag cccgagctac aaaaggtagc tcgtagggct 59460 ttagaactat ccccatacga tttcacaatc gtacaaggta ttcgtacagt agcacaaagt 59520 gcccaaaata ttgctaatgg tacttcattc ttgaaagacc ctagcaaaag taagcatgta 59580 actggagacg ctatagattt tgcaccatat attaatggta agattgattg gaaagatctg 59640 gaagcatttt gggctgttaa gaaggctttt gaacaagccg gtaaggaact tggtatcaaa 59700 cttcgttttg gtgccgactg gaataattct ggtgattatc atgatgagat cgatcgtggt 59760 acttacgacg gtggtcacgt agaattagtc tgataataaa tcaaggcggg gtaattcccg 59820 ccttttaaag caaagggggc tttaaaaact taatgatagg agaaagccat gtttgcagaa 59880 ctattcacca tgatattact aggagtatgg aaaataagtc tagtagtttt catcttaatg 59940 ataattctca ctgttgtcgc actagttacc caaaatgata cattaaagaa agtcgttcat 60000 gggctagagt atataattat gggcacattc ggcgtttgca aatgcaactg ccagagaaat 60060 acgaaatatt gttggctttg gatggagtta gagaactgca tcacagtagc cctggctgtc 60120 tcattcggta tgattcttat ggcccttact ctagcattga tacccctaat gttagctggg 60180 ggagtcacgg cttatttcac ccttttctcc cctatactta tgtattcaat atatccaata 60240 actatgtact tagttaggaa gagattctca catcaagcac actaatataa aaaagtagtt 60300 gactttccgc tgaaattttt atataatatt tgtataaatt gaatcagagg agtttcacaa 60360 atgtcagatc gttactatac tcagatggct aaccattaca atatgactcc gtatgaattg 60420 aacatcgcat tacgtgacct cgattcacca gagcgtgcaa aacttgagaa aaaggtggga 60480 attcgtatgt ctagcaaggg caagaaactt actcgtattg acttaaatac catgctaatg 60540 gaagagcttg gcgtgaatat tgaaggtcag aagttgcctc tcaatgttct tgaaacgatg 60600 ctggataaag tcaaaaagaa aacctataag aaagtacaag taccggaagg caggctaaaa 60660 gccccttatc aggctgctgt atctgagtgc ttaggtgcta ccgcagatct tagcactgca 60720 actgttaaag tgatgcgtgc gtttttagag gcagttaata aattgtgatt acccaagaaa 60780 gattgaagga gcttctagag tacaatccag atacagggat atttatatgg aagagccgaa 60840 agatagatag aagtggtttt aacaagcaat tcgcaggaaa agttgctggc aatcctcaaa 60900 ataacggtta tatcaatatt actgttgatt ccgaagttca tggggcacat agattagctt 60960 ttctttacat ggaaggctat atgcctgcaa gggtagacca cgaaaacatg gttaggtctg 61020 ataacaaatg gcttaaccta cgagaagcta cagtctctca aaatggtgct aatgcgttaa 61080 agtattcgaa caatagttct ggatataaga acgtatactg gacaaagaaa acgtgtaaat 61140 ggcaagtaca ggttatgaaa gatggtaaat cctactcggg aggatacttc acaaacctca 61200 aagatgctgt agaaactgcc aatgagttaa gatttgaatt atttggggaa tttgccctat 61260 atgaaccatt taaaggagat aaacatgagt aaattagtat atctattgaa gggatctacc 61320 tgcaatccgt gcaaaatgtt cgagcctgta tttgataaag tagttcagga ctacagtcta 61380 gaagttcata aagagactga taacacagaa cttatgcaga aatttggcgt tcgtcaggtt 61440 cctgtagtag ttttagcaga tcgtctcccg aatggtcgtg tggaagccaa ccacattctg 61500 attggtcgtc aacttcgtaa agaaactatg catgacgcaa tcaaaaactt cctggaaaat 61560 aatccagaag attaataaat ctaacccaga tcttagctga tctgggtttt ttattacttg 61620 ctttctgcta aaaattttga tataatatat gtataaattg atgagaggag tatcacattg 61680 aattacgcat atcttataat acataacaac gtggtctgtg gtactcgtgc tgtagaaatg 61740 ggaatcacag aagaaaaata taagtcactg cctgaaaagg agcaagaata ttatgttgag 61800 caagctgctt gggaatatgc tgaggcttat cccgaagaac gtgaaggccg tgttactatt 61860 attgttactt tggggctggt gggctgtgat accgaagttg atacagatat agaaacactt 61920 gaagaatggg aagagttgga tattgcagaa caaaatgcta tcatccatca atctttctgg 61980 gaagcagtag actgtcacgt tgtctttgag ccaaacgata cagaagctga aaaacacact 62040 aattggatga ctcgataatg aaaaaagaat ttaacctgca cgaaatgtta gtagtaccag 62100 acgatgttaa cctgttcttc gtaggagata ttcacggctg taatgatatg ctggaagatg 62160 ctcttaaact agcaggatat aaagaaaagc gtgactatgt tgtctgtgtc ggcgacttaa 62220 ttgaccgtgg cccgcagaac ttacaggttc tagccaaatt cttatataac ccacgcttcc 62280 gcagcgttcg tggcaaccat gatcagttta tgattgctgg tgactacgcc aactggatgt 62340 ataatggggg aatgtgggca atgaatgatc tggataccga tacaattaaa ggtatcgcag 62400 aagatatggc agttaaaatg ccagtattca tgactgtact ccatcgtggc aagaaatacg 62460 gtgttgttca tggtggaata ccattccaat ataaagatgc tggattcgat gtacatacac 62520 ctaattggga cgatattatt gatcatatcg ctgcaagtga aaatgatcct aatgaccatc 62580 ctagttatta tattgaacca tatttgtggg atcgtgatgt aattcaggaa attggttttc 62640 atctatctaa gcaaggtgca gaacatcctt atttccagcg ttatacaggc tttaaagatg 62700 aattgacggt ggaagtacca gaagttgaaa gtgtggattt cgtattccac ggccacactg 62760 gtgttcctta cccgattcta tataagaacc gtgtttatct tgatactggc ggtgttttca 62820 acgggcagtt gacggttgcg caagttaatg atgaagccgg taaaatcatg acatttacca 62880 cagataaaaa tgatagctgt ggtgtgcaga gggttttata atgaaagttt atttttggag 62940 tgatctccat ttagggcatg ttaatattcc taagtttcga actatgttct ctagtatgga 63000 agagcatgac gaattcatca tggatactat tacatctatg aaggataagc gtactgtatt 63060 tttcatttta ggggacgcat ttgtatctag aaatggtctg gagaagtttg aaaaactatt 63120 tagcaatgtt aggactactt tagttctggg aaaccacgat ttggaacgag aaggcttatc 63180 ttttacagac ttagtaggtg tagtagataa tgtgcagtct ttagttaaat ataagaactt 63240 ctggctttct catgccccag tgcacccagg agagttaagg ggtaaaaaga atttacatgg 63300 gcatactcac tttgaattaa tgggagatcc acgttacatt aacgtttgtg ttgagtacgc 63360 aaaatctcca gtaagattgg aagatattat gagtggcaaa tatacctccc atgataaaac 63420 tagttggatg ttggaatgat aacgcaggaa aaactgaaag aactcgtaga atattttcca 63480 gagactggag agtttatatg gaaagtcagt aaaggcccca ataaagcagg tagtaaagca 63540 ggcactttat ctggagatta tattcgtata gtcatagagg gaaataataa gttagcccac 63600 caatgggctt tcttatttat gaaagggtat atacccgaat taatcgacca taagaataga 63660 ttaggcattg ataatgcctg ggaaaacctt agagagtcta ctaaccagaa aaatctacaa 63720 aatcaaaaga aaaagaaaag caagtctgga tattctggag tatcctggaa caaaaaagca 63780 cagaaatggt acgtacaagt taccaatgct gatggaataa gaaaatctgg tggttacttc 63840 ccttatatag acctaagctt agccgttgat aaagctaata gtatgagagc tttataccat 63900 ggagagtacg ctatagtaga aaactttagt agatatatac caccaataga cgaactaaat 63960 aaataattta cttgctaaaa tgcctaagtt tctgtataat tactttataa attgatgaga 64020 gaggtttcca acacaatgaa tactaaaatt gataaagcac tcttaatcgc aacagctgat 64080 aagtttgagc aagttaaagc tacttttaag gcagtctttc agtcctatgt tcaagacaaa 64140 tcgaacccta tttctgagcg tttgatgatg tgggaatgtt ttgcttgcaa tgctctgcta 64200 atttccgatt ataagggaga agtatctgag gaattgcatg agatctttat taatgaggat 64260 actccacgct atcagttagt gaacttccaa gatctagctg agcaagttat tcctgatgat 64320 ctttgggaca agtattacgg tgatcctgaa gaagatggta tggaacctga gaagtgcatc 64380 gaactgatct gtaaggatca tcctgaaatt gctgagaaat ttgagttagt atttgcttct 64440 gagttctcag gtttcgttaa cgattggtaa atgataacac aagaactact aaaagaacta 64500 tttgaatata atcccgatac aggagacttt ataaggaaag tcagtaaggg tagagctaaa 64560 gctggagcta tcgctggcag tcccgataaa gatggttata tatttatcgg gataaacaga 64620 aaaagatatg cagcccatag attagctttc ctgtatatgg agggttatat cccagattta 64680 gttcatcact taaataatat aagaaaagat aacagatggt gtaatctaaa ggatgccaca 64740 tctcaggaaa ataatcgtaa taaagtcgca caatctaatt ctggatatct aggggttagc 64800 tggaagaaag atcgccagca atggagagtc caagccagaa attctgaaag tactccagta 64860 catggtggct actttagata ccaagatcta aatttagctg ttgaagcagc taatatacta 64920 agggaagagc ttcacggaga gctagcagtt aaggaagtct ttcgcgggtt gcttccagca 64980 ttagaagaat taaataaata atttacttgc taaatgtcca gaaatcttat ataatatatt 65040 tataaattaa tgagagagga agttcaaatg gaaaacatga ctaaaactga aatggcaaac 65100 gtattggcta tcctgttgga catgcaaggg tttgaaggtc agctcatgaa gatgtctatt 65160 ccggcaatga aaaagatgta cgattctctg aacaagaatg ctatggcatt caacctcgca 65220 aaacaggaag cacgttttgc gaaagagcat caagcaactg ccgaacgtcg tgcagcatct 65280 tttgagcgtg aagttaagca actaaaaggt aagaaataat gactaacgga aaattcttac 65340 ttgtactagg gctgaatgcc ctagtttccc tggctatcac gcttggtgtt accaatttca 65400 cgctcaacca gcgtgagaag atggaggact tacctttatc cttctcagcg gttgaatata 65460 ctaacccaga agcagatgtt atacgttacc aagctaacct agacgagggt ctttcgaagt 65520 attctggtgt ggaagttttt gatgcgtata aactgaatga tatagctgaa gctaagcttc 65580 agattgaaga aggtaaacgc ttctgtaaag caagtaactc atccctagta aaaattaagt 65640 tttataaaac tataagccca tatattgaat gtggcaacgg agtatttaaa tgattacaga 65700 aattttaatt ggattgctgg ttctcacgac tttagcagca attggcggaa tcattggcat 65760 tattaatgct aataaaaata tggataggat gcgcctcacc aatgatgggc tagttaaacg 65820 tctcgttgaa caggaagaag atatcgaatt agcacaaaga cgctcagatc ttcttaaaga 65880 aaagctaacg aatattgaaa ctcttgttgg taacactaaa cttcctatta aagtcctgcg 65940 cgctcaggta atcacggaga ttaagaaatg atgttaatta tcttagcttt ttatctggtt 66000 attgttggtg ttctggttac taagtatcat acctggaccc cgaagaacgt tggtaaagta 66060 gcactgttta ttctgccagt tcctatcatc atcgttgcaa tgctgttcac aatgttggcc 66120 ggtaaagtga caaaaactga cgttaaacgc attgtagacg aactacagca atcttctgac 66180 atgatcgagg atattctgaa agatgaagct taattttagt gaattaaccc tgctggacga 66240 gtttctaggt tttgttactg aacgtgggtt tattaatgta gatcctcaga cactagaagt 66300 cttgcaatcc atccagaata aaactaaaac agatctggaa gacttatggc gaccactttc 66360 tgagctaact cctcttaata tgagagtgat tgttaaaaat attgaaactg gtgaagaacg 66420 tgagatggtt cgtaaagaac tggctaatag ttattctcca gagtccctag tgatgcatca 66480 tgacgatgaa ccagaaacac tttggacagc ccattatatt tggcgtctgc cttgattgat 66540 ttaacaactg ttacggcaat cctaatcttc ggtttagtgg ttgccgtttt cataatcgtt 66600 gagcaggcca aagtaatacg gagacttaaa aatagatgat taatttttat aaccctaact 66660 tgcagaaagc aacgtgggat tactttataa ttctaccagc ttttatgtta agttgtgcaa 66720 tcattaacat tatgtatcaa gaagctggtt ggacagtcca agatttccac atttcacaag 66780 ttcttccttt catgtatgaa ggcgttatcg cagcaataaa tggggttcgt aaatgacagc 66840 actgcaaaga ttacagaatt actggtttga tggccaattt aatgatttat tcatccagtt 66900 gtttgtcgaa acgaacggac gttttaatta ccgcttcttt aatactttcc atgaaacgaa 66960 gttctcgcat acagagatta acgcagcgat tcaagatctc accggatcta aggtaattcc 67020 ttatcgtgat gtagattttg cacctgactg ttttggtttt gagctgttca agaaagcgta 67080 taagttcggt aagtttgaaa atgcacgaca ttgggttcat gatttctggt ataataccga 67140 tatcgttcct agccgtgtac tgatcctcaa ctggattgct aaacaacatc cgccgaaggc 67200 acaatcggca ttccttccaa ctgatacagg aaatctttac catgacacaa aagaaaaatc 67260 cagtgctaga gcagatgaag cagttggaga agcagattga ggaaggaagc gttgatggac 67320 agtcaattgt caatcatgct tttgatctta cagtaggatg tgctaatccg ctggtggcag 67380 gtgaaacagc agatattctt gggcttttaa tggcagttgg ccagttaatc gagattaccc 67440 aagaagagct agacaatgac gaaaaagtaa ctgttcatga tgttcttggt gctctagatc 67500 tgattgttaa tgcttatgcc tataaacgcc gccactcaat tgatgaatat ctagtttctc 67560 gtgagattac cgaatctctt ctggaaactg ctgatatggc cttaaacggc aagcaacttc 67620 actaatccta gaggccagga cttaattgtt ctggcctttc gtgtatacta tgagagataa 67680 aataaacaaa ttactttatg aggaagcatt acaattcccc ataaatagat tcactaaatc 67740 agatggatct attaatagaa ctaaacttaa acaacttcat ccagacttcc agcaagacgc 67800 acttaatcta atctttatca ggagagctgt agaagcacat ggtaagttct ttggatacga 67860 gagagttaag tataaaacta tgcaacagca agttgagatc tattgtccag atcacgatgg 67920 ttacttctta caaactgcac ggtctcactt agaaggacac ggttgccgat tatgtgcaca 67980 taaagttgtg acacgcttaa cagaatatgg agtgtacacc gtaccagcac ctttccacaa 68040 atttgtagtt gacggtgagc acataatttg gtataataaa gatcaaaagt taaaacagga 68100 gttaaagaaa gatggagtac gtagtctggt caaaccccct agctctactc tcccacaaac 68160 tgttcaagac gacgtgggga aagaatgaga taattgttaa cgagagtcct cttaatcatc 68220 aacatccttt agtcccagct ctctacaaga ttattataaa tggtacagaa tacctagtag 68280 atgatttcgg taaagagttt gtacaagaag ccatagacaa caataacatt tctataggcg 68340 aattaattaa tagagcatct aactttgata agagttggat gttaatttgg agtaactaat 68400 ggaatatgta ttaattatct tagcaattgc attagtattt gctattaaca ctgctgtaca 68460 taagactcgt gaagccaatc aggttattga cgataacgtg aagttgcgta gcaaagttac 68520 taatctcaaa caacagcgtg aagctctgaa gttattcgtg gctcaaggta gcctagagca 68580 tgcaccggaa gacttcatcg tatatcttaa acgttacata ggaatcaaat aatggaaaca 68640 ttacttcttg tactactagg cggttcactc ttaaccattc taggcttgat aattatatgt 68700 agtcgcctca atgttaagaa tctagaatta aatagtgaga acgagattct taacagagaa 68760 attaaacaac acaatatcgc agctcgtaag ctgttagata aactggagaa taaataatgt 68820 ttacacaaca aaccattaaa gtagttgcag atcatgaact cgacacatgg gattacagct 68880 ttaaagatct gactgctaca gtacagttta aatctatgca actgtcgttt ttgcacattg 68940 atcttcaggc ttgcaaagag tttcaggcta aactcatgaa tgctcgtgct atctttggca 69000 cggctcgcat cccggctgat aatctgatta atgcactgat cgatgcagga tacaaactcg 69060 tgaagactga agtgaaccgc ccgagtactc cagtgactat tcgagatccg tggcagtcgg 69120 gaccaatcgc aatgctgaat aattccgata aaagattaat gtcggatatg cacaatgttc 69180 cttgtggtgg cgtggtaagc aatacgcaat catataaatt tggggaagca tctagcgtag 69240 acgagtggaa taaagctggc gttgtaatga tgggctttgc aaaagatcgc ggagcacaag 69300 aagactccgg agtttctagg aactcttata ctatccattt gagcaatcct agcaaagatg 69360 gtgagcttga agaaatgtta acgaaagtat tcgaagctat tgatgcagat aaggctagag 69420 gtcgttaatg gatacactgc tctcttacga acatgaagac tttagtataa aacttattaa 69480 tacagatacg tttggtattt atttaaaaga agaaatcaga catgttttct ttgctaacca 69540 acaagatcta cgtactatta ttgagttaat aagtaaaata ccaaaagaag cgatcttgca 69600 tcactgttat ctagcagatc ttatcaaaca gttaggttta gtatgcgtag atgtaatacc 69660 acgtaataaa aagtaactaa tacacaagcc tctacggagg ctttaaaata aaaagcaggt 69720 catctgacct taaaaaggaa acacaatgag tacatataat tttaaagaca aactgagaca 69780 aaatgctggc aaaggaaaag ctaaggaaac aaataatatt ccaaatgcta agatcctacc 69840 gttccctgaa ctaacccaag aagaaaaaga taagctagca tttatgtttg atactttgct 69900 aggactgaat ggacgtttat actctacgct ctcgttaaat gagaaacgtt tagtagagtt 69960 ctttgggaaa cgttatcaag ttacatctaa gggtaacgct cttaaagaag gagaaaaacc 70020 ttacacagtt attaagatta gtgcatatgc acatcgtttc ttaattcgtg cgctagagac 70080 agaatatttt acgctacctt catttagtgc attccacatc tttgaagatg aacgaatgaa 70140 tattgatgat ttatatggag atcgttatca atattctgga agcccgttcc atgatgcctt 70200 aagaagctat ccaaattgtt atcttccaat tagagcatca caaggtaaag caagtgtctt 70260 tgcagtatac tcagggtcta aagcatcgga tctagaaact tatatttata aagaacgtag 70320 atctgctatt ccagaagaag cacgttatga aaatccaatt ctaccaacta aagaagtcaa 70380 ggaaaccaaa acttctacta ctcaaacaga atctccttat ctgaagtttg agaaacaagt 70440 tggatgggtt ctagaaatgg gtatagcttc accgcttgct gcagcattta tcaataggta 70500 tctagaaatt cctgacgagt tccacacacg ttataataca aatattcaga tcacagatga 70560 attaatagct attgcaaaag atacacctac taacactgag gcgtatcaaa aagcatacca 70620 agagttactg taagatcctg catgtctcgg tgcacaatac ctgtaccgag actcaaatac 70680 aaactctcgt tgacagataa ctaaatgtat gttatcataa ctctatgagt taatagaatt 70740 aaccatttag agttgccaca cagaaggtgt ggtggttcag gaatcttaaa ttactgactt 70800 gtagcgtagg ttacggagcg acaggacaag tcaacaggta atttaataaa gattactaga 70860 aacatcacga gaccttctgg cgggggcaaa gctctatatg agaaccctgg gggatcttat 70920 acttatataa aaactaatac tgttgaatag ataaattaac gttcaagatg atacaccggt 70980 tgccgctcat ctgctaggac ggcacaacac tttctagtag actcctaagt ttctaggaat 71040 agttaagcac taactctaga gtttacacta aactttaaat ctgtttctca cgcattctag 71100 ggcagttttc atattcctag aaactctagc aataggcggt ttaatacagg gcggttatgg 71160 ggagggcggt tatttctagg aaaaataatt gactcttgtc agtctggaaa ttgctatcgg 71220 atttgcacat tatgtttcta gaaatttatg gtcggatttg cacattgata ggagtctcca 71280 aagtttttag gaacactgaa aatagcagca ttatttcggg ccgaaggccc atgattgcct 71340 gagtttttag agccacagga attttcactt gtcaagcgat tttgccccat tttgctaata 71400 aaatttcgct ttatctccac taatctcaat tctagccaaa acatcccaca ttatccccaa 71460 aaatgcagca aaccggtgct aaagcaccat caccgctgct ccttccgtag ttcctcccaa 71520 aatctcatcg gattcgcact tctccccaaa cccacacaat cacacaccct ccaatcgaag 71580 tcacaaaaat cttgtcggat tcgcacacct taacacttgt cggatttgca catactctag 71640 taaatccagc tggattgcat cgcagttgcg gattgctgga gtttttagga aaattcatcg 71700 tcggattcgc acatataaaa aatatttgcc atcggatttg cacactacca aaaattgtcg 71760 gattcgcaca tacacgcccg cacgtaacac ccgtggagca atgtcggatt tgcacaaaag 71820 cgcagggcaa aatgaaaatg ccctgctggc cgaaatgcga atgataatga gaacgattcg 71880 catttgagaa gttgaatgcg aatgagaatg atttgcattt ggaaagcgga atgagaaggg 71940 ttatcattta cagaattgaa tgcgaatgag tcgcataatg agaatgatta gcgtttagaa 72000 atggaagtga gaaacactgt tatttaagtt atccacaggt ttattaacag cacgatttga 72060 cttgacaaaa tcctagcgat tttgaggcgt tttcaacaga cttatcaaca ggttattcta 72120 ctgtataaat atacagtata ttccagcagg agaataacct aatataggtc taaaacgctc 72180 tcaaacgcat tctaacgcgt ttatttttgg aggaggtatt ttaataaggg gaaatttgag 72240 gaagggcaga aaaggcgggg ccgatagttg cgccccgtta ttattagcgg tagatgtatt 72300 gttgccagta gcggcggata ttgtcgccac cgtcgggatc attagcagac ctaatattac 72360 ctataaaaat atatagtttc ataacgccat cgctcctaga atgagaatga ttattattaa 72420 cattgtgaag ccattaccaa atcgtaaacg ctgggaatgt ttcccagcgg tattattaga 72480 tttatgggat tgcgttttac gccaccagct attcataata aagccgccag ccatcattaa 72540 acatttcacg gctataaaat tcatgattaa atttatagcc atcctcagtg gtgatcactt 72600 catatttcat gaataaatat tcatggctag aaaaataacg atgggttact tttttgcctt 72660 ctagcatagc ctttacagct tcatttctag tcattgcgcc acctttcgaa acgccgtttc 72720 aatactttca gcggttgatt gaatatatgc gcctttcata atcaggcgct ccgtacatac 72780 tttgcggaaa atgtcaataa attcattttc attttccgct attaatgctt ctcccataac 72840 ctgaacgtca caagacccaa aagttaaaaa ttgaaacgct agttcacggg cgacaacggc 72900 agaaatttta taatgtgctt tgccctcata gatattaacc acgcctttat aaactggtga 72960 aagctgattt aaatagtttg ctaataatgc cgaacggcaa ttagtaataa agcgattacc 73020 ctcataaata accattaaat gatcctcgcg tcacggataa ccataatcac tagcgtgatc 73080 aacattgcag cactagacac gccgcaaaca aaagttaaca ttttaacaat tgccccttcc 73140 attataatac cccacgaaat gcagcgtaaa aagtggcaaa gtgtttaacc gtttcggcta 73200 ccaaattgcc gcgcttgtca aatttctttt ctgtaacacg aaacgcgttt ttgttgcgtg 73260 ctacatattc gattttacag tctggacgct cgaacgtgtt aacatccacc agcttaaaac 73320 ctttagaacg ggcaaggctt acatttttta acatttttct tctcctttat tcatgcagcc 73380 cagcaaataa gaaaatacag cgatacccgc gccaataata accacgtgaa cgccgaaagc 73440 tatggcaaaa agtgataaaa tagcgcccaa gatacccgct agaattgatc taaaaataac 73500 catttgcata actcctagtt agggaataag gggcgaaagt aacgccccgt tattaattac 73560 attaaaacaa tttttgccgc ttgctttttg gtgcagtggt tagctttcaa ttcatccagc 73620 aaaaatccgg ctgtttgctg atcaatttta tcattgtcgt aaagttcctg aatggtgata 73680 taagttttac caatcccaaa ttcagaaacc ataacgctga taatagcggg aacggatacc 73740 cattccatac cattaaaaat tgcagaagat gatgaatgta ccattttaac gcctcctaga 73800 aatcagggaa aatttttgat ccaccgtttt ggtgtaaagg cattataaca aaaaaggccg 73860 ccataatagg cgacctttac aaaactttac attagactat ttctaatgct tccacaccga 73920 aacagcgttt tagttctttc tgtaaaacgg catattcatc ccaacgggaa ttatcaggaa 73980 tataaacaag ctgttttaat cgatttgcat ataataacgc cttgttacca attatttccg 74040 cgccaccgtg tttaataatt agtttcataa cggtattaat aggatcgccg ccatttacat 74100 aaataccgga tttactcaat tgataatgat aaatactcgc ataaacatta tcattttcaa 74160 tccattcccc tgtttcttca tcttcttccc catcactacc gaaacgagaa gcattatcat 74220 atgcgtgttc gtttgcgaaa gcgtccagat cacagccagc gggaacaaaa tgttcctccg 74280 tccagccttc cccgcaatag ccagtatgac aatcaactaa aactatcata tcgtgtttag 74340 cttcattcac actttctagt gaaaagccat atttttcttc tggcataact agcctcctat 74400 taattagcag ggaaataaag agaatttgct acactgtaaa atcctttatt taagaaaatt 74460 acgtcatcac ggactatttc atccattcca taatccagca aagtttcgtc tacaaaaata 74520 catagccagc cataaccacg atcaaagaaa gcatgataat tattcgcgct ttctacatgt 74580 tcccataaaa agggacggcc ttgtttttcc catataggaa tattgctttg atcgtccgaa 74640 taattaacat gtgggctatt cggcattttt tcggctaatt gcgtcaacat gatttgattc 74700 agttgcataa taagtagtct cctaacaatt aacagggaaa tagtaacgcg gggaaataat 74760 ccccgctagt tattactact cactttcggt attagtggta aaagtagaaa tgtcaatacc 74820 ttttaccatt tcatcgatca tttccgcaat attgaaactc tgtaccattt ccagcattgc 74880 cgcttccatt gccgcgcctg ctaccgcttt cgctttctga ttagtcgggg cgtattggtc 74940 aacagctaca ctcagcaggt tggcaatcac tactcgacca atcggcgtat caatataacc 75000 tttaatcata aatggcgctt ttttcgccgc tactttcgtg atttgagtta atgcaatttt 75060 acccgcttcc agttttgcag cgtttacaac ggcggattta ttagcagcaa cgatagaaga 75120 tactttagtc atgatatttc ctttattttc agtagtttgc gcatttgcgc ttgaagtttg 75180 agagtttaca gcctgttttg ctacattgtc aagtggtaaa agtgtaaatc cagcgtttgc 75240 aacgtttgat tcattgccat aacacaaata ttcgtcagga atcaacatgt taccgctagc 75300 gcgttcaaaa cacaatacgc cagttgtttc gattgcgtca ctatcagcac gtttaatggc 75360 gcttgtcaat tcgccccaaa tactagatag taaatcttct acatcgctgg aattaatacc 75420 atttacgcga gtagcgaaaa ccatttcttt ttcttgtaca ttgtaaccac gggcaatata 75480 gcttttttcg tcgtgaccta caatgataaa atcaatatcg cttccccgct gatttcgcat 75540 gatgtgatag tcacggttgt aaagcactag aacgcgggta gggtctagta attgtgtgaa 75600 atcacttaaa caataatgag tatcattatt atctaaaatt tcatcgtgaa tcttttcaac 75660 cattgccgcc gtatgttgta ggcttgctgg acttgatccg tctagcaggt tgcgcaaact 75720 acccttaata atagtggcgt ttgctggtac tgcaattctt acagtagaca tttctttgtc 75780 tccctcgtta atcggtgagt gaatgatata gatatttgtt cgctaggggc aagaaaaaag 75840 aaaaggtttt ttattccttg cctactgcct gtttttatga taaccgcgcg ttttctattt 75900 ctagttctct gtaagcgtac acaaggcttt attataataa gggtagcagt gagaaaacgc 75960 cgtatagcgc gttctaggcc gcttaaattt gattgcggtt gttactgcta ccattctacc 76020 ctgtatattt atacagtagg ataacctgta tataagtctg tgggtaagtc gccaaaacgt 76080 cgcctattgc gtcaagttat tttgtgctgt ggataacttt gtgaataacc taaataatag 76140 tggttctcac ttagccgata gtttcttata gttattattt cttattaatt tatatgtata 76200 ttatataata tgaaatagtt agttggctaa tgagattttc catgattatg agtctgctaa 76260 ctatttcggc tgttacaacc taccagcgtg attatcatcc tagcgattat tagtctgcta 76320 acgatctgat acacgtctaa acttatttgt caatgaaaaa taatagcaga aaatactttt 76380 ttgaaaaatc aagtggataa ctcattaaat ttcatttttg ctttttgtat aagttattaa 76440 cagggtctaa aacgctctgt agcgcattct aagcgcctca aaggaaaaga taggcaatca 76500 taaggggcac accgagaaaa cctcactacg aggattttgg tttttgtcaa gtagggcaaa 76560 gcaaatataa atgaaaaaat tacttgctaa aatggttgtt ttgatgttat tcgcgcccgt 76620 ttcatttaat tttaggcagt ctctggacgc ggtgcggtgg cgtttgtctt tacgtttctt 76680 tacaaaaata aagttgcgcc cttctcggta ttctcctatt atttatcacg tagggcggca 76740 atgacgaact acacggcggg gataacagcg attgacagtc tgtttgacct ttcaaaaccc 76800 tgccatgctc tttaaaaatt agggaacatc ttaaagccta tcggcggtaa acttatacat 76860 tataaggaat cgcagatatg atcaaatcaa aaaacgcttt agtgattagt gcaaaacctg 76920 ctagcgcatt agatagcaga acaaaaaatt tgttgacagt agcgcaacga tctgtaaaat 76980 acagattcaa gcaatacaag aaaggtaagc agcaaggtct ggaaatggtg tggcgtaata 77040 taatgattga tttatataac tcacatcaaa tcgaaaaaag tttaaaaaac gcttgacacg 77100 ctagccgata ggttttaaga tgttccccgt agtaactgct ctttaaaaat ttggataacg 77160 gtaatgttta ggctaactcc tagcggatta taatctaata gcgtttcaaa gtatcgaggc 77220 gctattgtgg aataatcccc aataaaccgg agtattaaaa atgcaaaacg ttattaccgc 77280 gccgaaagta ggtcaatctg ttttcattcc ttttgtaact aaaactgatg aattaaccgg 77340 aaaagccgaa cgtatcaaag gtgcggcgct tgatcccttt gataagattg aagcggtcta 77400 tgcggaaacg gaacgcagta acaacggcaa gcctatttat aatgttcgcg ttaaatctgg 77460 cgacgttgta aagatcattc agaaagatga taaatggcaa gctatagcat aaattagtgt 77520 tacttttata ttcccctgaa atatggggaa tatatggatt aacattagtt aatcataatc 77580 ccttaattaa taattgaatg gagtattaaa ttatgtctaa cgttaaaacc gaaaaaaccg 77640 caaaattctc ttggaacgaa gaaaacaccg aaaaggctgt ttctatgtat tctgaaatgg 77700 tcgctaaaag cgggattgag tttgcgaata gtgacggctt aaaagaaatt gccgccgccg 77760 ttggcgctgc aagtcctgtt tctgtacgtt ctaaactgac aagcgcaaaa gcctaccaga 77820 aaagcgataa accgcgtaaa gttggcggcg ggtctagtgt ccgtaaagct cactatgtgc 77880 gcgtcatcgc taaacacgcc attgattcag gtatcgtaaa agatgccgac gatctggcaa 77940 gccttgaaag tgctaaactt gaaactctgg acgctgtagc gcaattgtta ggcgtagcgg 78000 aggaagtaaa acaggccgca ggtgaataaa ttttaattag cggggaatat tccccgctat 78060 cccttaaagg agaataatct tgatctttta tcctagcgaa gcgtttattt taggtttatt 78120 tattattgcc gccgctttta ttctgaatta tacgcggtca attctagcaa taatcggaat 78180 taataacgtt tacgccgcaa ataatttccg caaaaacgcg aacattcaaa aatatctgtc 78240 aattatcttt cttacaattg gcattgtatt tggcttttgt tctagtttaa tgccgcttaa 78300 ttaaatgttg caaattataa cgccttttaa attgtcctca cttcaggcaa ggcgttatat 78360 ttggcaatat tgccagcttt aatctttatc cctttatagg agttgaatta gttatgaata 78420 tttccgcaga aaaacaagcc cagatcgtta aacttgccgc tgattttaat ttctacggta 78480 aacgtttacg cgctactaag ctggaagttt gcgacgatat atcaaaagct gtttacgata 78540 caacgaaaca ttcaaccgca atttgcgact ggttggaagc aaataagcca gcaaaaccga 78600 aagcggcaaa agctgaaaag gcgattaaaa atgatgatcg ccccgaagcg gcgggaattg 78660 tttctagtac cgtggaacag tgggaagtta aatccggtcg ccgttttatt ctaacgtcga 78720 ttcaaaataa tactttcccg cataaaaact ttttagcaag tctggaaaaa ttcgctgaat 78780 atatcggcgg tgaattgctg gttagtaaat tcatttacaa taaaaacggc ttccagaatg 78840 gagaaggtaa cgaaggcatt aaatacgatc ccgcatttaa taaatatatt tgcgagaaaa 78900 acgtattttt aaataatcgc cgttttgctt tcatggctga aattaacgtg ttaccaacgg 78960 cagactatcc gctttcagga ttcgcagaaa ccgccacggc tttaaatatt gaaggtctgg 79020 caattggcca cgctaaaatt accgctgaaa gtgtgccagc gttaaaaggt gaagtagtgc 79080 gccgtatgta ttcaaccgga acggctacgc ttaaaaacta cattcagcag aaagcaggtc 79140 aaaaagccga ggcgctgcat aatttcggcg ctttgctggt ggagtttgac gacgacggcg 79200 aattttttgt gcgccagctt gaaacaatgg acgaaagcgg aatgttttat gatttgaata 79260 tttgcgctac tcctgccgga tgttatgaaa cgtctggaca cgttttaggc ttgcaatatg 79320 gtgatattca cgccgaaaaa ttagatgatg aatgcgccgt tgcgtcatgg gctagtgaaa 79380 acagcttgct tgatattctc aagcctaaat atcagtttgt ccatgacgtg cacgatttta 79440 catcccgcaa tcatcataac cgcgctagtg gtgtattcct tgctaaacaa tacgccgccg 79500 gacgtgacaa agttctagat gatcttatcg ataccggtcg cgtgttggaa tctatggagc 79560 gtgatttctc tcaaacaatc attgttgaat ctaaccatga tctagcgtta tctcgttggc 79620 tggatgatcg taatgctaac atcaaagacg atcccgcaaa cgcggaattg tatcaccgtc 79680 taaacgctgc gatttacgcc gctattgctg aaaaggatga tacctttaac gtgctagatt 79740 acgccttgcg gaacgtcgca ggatgcgaat ttaacgctat tttcctgact actgaccagt 79800 cgtttaaaat cgctggcatt gaatgcggcg tacatggtca caatggcatt aacggcagtc 79860 ggggcaatcc taagcagttt aagaaacttg gacgccttaa cactggtcac actcacacgg 79920 ctagcattta cggcggcgtt tataccgctg gcgttgctgg ttcccttgat atgggctaca 79980 acgtgggcgc gtcaagctgg acgcaaacgc atttaattac ctatgcaaac ggtcaaagaa 80040 cattgattga ctttaagaac ggtaaattct ttgcataatt aaatttaatc ggcggttgaa 80100 atataccgcc attaataaaa ggtttattaa atgaatattg tattacctaa aacactggat 80160 aaaaagcagt atataacgcc ggatgatatg gaatgcggca aggtatacca attcgattat 80220 atgttggcta gtccgacgaa tacaggttgt attattgcag ttgatacccg tgataatgac 80280 gtattaacgt ttaatgatta ttctgacttt atggaatggt tagatgatca aagtattact 80340 aataacggcg aattaacgcc gtatcaatta acaattgcaa ttaaataaat taatcccgta 80400 attaaataat ctggagtcat aaacatgaaa aaccaaaaca ttccttttga tcgtgctact 80460 tcttccatcg ttttagttta ttctaatggt gaacgttatc acgtcgaggc ggggcaagtt 80520 aattttgatt tgctgaatta taacgacgct ttgcaggtta ctacctttgc ttatgatacc 80580 ggcaaagtgg caagccatac aaaagcaaaa ggcgtttacg ctgatacttt gacagtgcaa 80640 acaattatta ttgatgcgct taaatccggt ctagcgtttg cagttgttaa gccgtgtccg 80700 gtctgcaatc ccgaagctag cgcgatggtt tacacttgcg cggggattcg ctctagcgta 80760 catggtgaag atcttagctt tatcgccgat gctctcgctt ttggtctggc gttctaatag 80820 ttaattgaac attgccagct aatctaatat aattagctgg caagattgaa ttaacctaaa 80880 tcccttaaat ggagaatgta acaaatgaat caagtcaata tgaatattac ccgcactttc 80940 ccgcatattt cccgcgttat gatttgggat ttagacggta caatcattaa ctcaatgcac 81000 cgtgtcgggc cttgttttga tgataatggg aatttagatt taaataaata ttcccgcgaa 81060 gcctgcaagc atgatttaat tatgcaggat tctttattgc cattggttga atatatgcgc 81120 caatgtatga atgatcccaa taccttaaac cttatttgta ccgctcgctt aatgggtaaa 81180 tctgattatt atttcttgcg taaacaaagt ctaagagggc gcggaaatag taatattcgc 81240 gtattttccc gcgatacatt acataaatac tttgaagcgg gtaaggttag cgaaatatat 81300 cacagcaaag acgctgtata taagtcttat tactttgaac tgtttaaaca gctatatcct 81360 aacgctgatt ttacgatgat tgatgatcat aaaggtgtat tatcagcggc ggcaagtgct 81420 ggatttaaaa cgctggacgc tcaagccgtt aacgatatcc tatcaatcgg cgtaacattg 81480 atcggtgaaa cctttattga tgaatcgctt gatgatgata acgattatca attcctagcc 81540 gaacgtttaa aaatgtgttg ggaaggtatg accgaggaag aacgcgcgga atatagtacc 81600 acaccgcaac aattcattga taaattaaaa gttgcctaac aattaaaaat aaaatagtca 81660 cactgtgtaa tgataacatg gtgtgactat tagcttgcta agtaaggggg gcggtaatga 81720 gactcattct catctaggga acctctgggc acaccttcat gtgtaatctt atgagatttc 81780 gagaattggt aattagcgct aacttaggag gaacaccggt ctactggctt gtctcctgat 81840 tctgacatac gaaatctcct ccgcgatgcc cgagatccat ccgaccactc tcgctcaaac 81900 tcctcccgtt tctccgcaaa cttccgctcc ctttccgaaa gctcccgcga tactcccgca 81960 atatattcac gatgcttatt aatcgccccg agtgttccga agattcccgc aagcaccccg 82020 ataatgaaga tagctacaaa gctaaacatt atttatctcc ccgcagctta cgttcgatac 82080 gagctaggtg gttatctaaa atgcactgcc cgataattac cacaactagt aaagcaataa 82140 tcactacttc cattataacc accccgccat agctgctaac caaaagagta caaagataaa 82200 gaataataca gttccgaatc cgaaactatt tatccaactt tcgtacactt ccccgcgtct 82260 gttctttctt ctgttgtcgt ctgacatctc tttgcttctc ctgtagtcct ggtatttgga 82320 atatcctagc aattgcacga accattatga ctgtcccgat aagatccatt acttcatcga 82380 agccggactc ttctagtaag aaggatgttc ccatcacgtc ttctattgta tcttctatga 82440 cgtcttgaat gatttcgtta atcgcatcat gtattgcttt agtcctggga tctatttcaa 82500 agttaacagg ttcctgaaag gacttttcta catcttgcat tgtgtttgct acctgtacct 82560 catcccagaa ataaaaattt ttgtttactt cgagatcttt ttactttata ctaattacaa 82620 gtgaccagat tgttctagtt taaatttgca atcgccatat ttgagatcaa actgtgacaa 82680 atcccaccac ccaaacttag tgcgtaactg agttttaggg aatcggtagc ctacagtacg 82740 tgcttctcga atatctaaag catagtgttc ttcaccaaca tgaccataag acttcacata 82800 tgccttacaa aatggctcgg gagctggtac acaacccgtc aacactactg ctgcaactac 82860 gatagctagt aactttttca taatcgttcc tctgtttaat ttataaatat attatagcaa 82920 aatagaggga tctaagcaaa tacattttta aagaaggaga ttgtatgagt tttttcgcag 82980 gaaaatttag tgatggtaaa actgtattat cgctaaacgc tgctaatggt ggtgatactg 83040 gtgaacacta ctctccaaat actaatagta tctttcactc agatatgcct ttcgtcctgg 83100 ttgatgggac ttatgaagcg gctctaggta atgcaggtaa tggatacttt gttactcaga 83160 tgccttggga tattataaat attaaatcca atgatccggg tagagttata ttaactgcta 83220 ttgagattaa tggtacccat agaggattcc tcaatggtac tcaatctcag gttggccagt 83280 tcttatcatt ctttgaagac ccaccacgtg ctggtgctga attatctgta acatctgctt 83340 ttgcgatcgg tgatagttta gctcatggta cctatatcta taatggtggt cttggtcatg 83400 aggaatctat tgctcggcaa ggttctggag gcagtttact acagtcctct ggtttccaga 83460 tctttagacc tgggggtgtt tctgctggtg aagccattgc cagagcctgg agtcttatgg 83520 gttttccaac cggtgtctct actgttcctt tagatggtgg taacgcagat tattgggaac 83580 ctaactggca ggcgcctatt ggttctgctg gtagaggcca tgattggttt tatgtttgta 83640 gttctaatat tcgtggcttc gcaggtaaaa aaggcgtact accagataac gtaaacgttc 83700 tttaccaatc tcctgcatac ccagaaaaaa tatatgtttg tagaggttct acttctaata 83760 tggctgctca gtctgctaga aaggtatatg tacaggactg gtataacgtt acgccaacta 83820 aggttatttg gtatgtgcta aatctgcgct attctaatgg tagtatggga gtttctggta 83880 atccatttac cggttcagat atcctaatat ctccatctaa ctttactatt aaaggtgtta 83940 gcctaccaaa tacaggctat aaatttatta accagaatgc tttcggaaac ttaggctatc 84000 gccctgatat ggagtatgtt ggtaataacg ctgcgtatac cggagttttt ggtgataata 84060 ctgcacgatg tgaatttatc ggctctagta atggcgggtt gtggtctcct gttaactatg 84120 ggggtgcagc ttctcaaatt agcttatata aatttggtgt aggtaaacaa tggtatgtag 84180 atactaatac caactccatt ggtaatgaac atggacctgt gtggagtcct agtactgtac 84240 ccctcaggtt attcccaggc aacgttgcaa gtacttatgt aggggacgat ataactccat 84300 cctaccctgg tgctggtgac ttctacacac ctttggccac agtttggttg ggattaccaa 84360 atgctcactc tactgttata ttaactactg aagttattat gggtaatctt aatactgctg 84420 gaatgcctgt gcgtacttac ggcggtagcg cttggcaggt acaaggtaga cgtcaacaaa 84480 gttatacagc tggtgatggt gtattccacc agattcttac tctgccacct ggatatctag 84540 taccttatca cagtacaact tcctatagtt atacacaaac ttgggctagt aggcctgatg 84600 agtttgcttt ccgtaggaac ggatatattt atactattaa aaaccttggt aatggcaacg 84660 ttgagttggg tgttatcatt cacgctgcag aagcggcagc tgttttcttg ccaagactac 84720 gtgtaacagt tcaacgccta acctaaagga ggatatatgg caaatgatgt attagttccc 84780 gatctcatgt ctcccgaagg catggatgtt attgaagcct atttacagtg cggtagtgat 84840 gttcctgccg ccgctcgtag tcttggaatg tccgagattg cattccgtga tataatgaat 84900 cgtggggaag ttaaaaacta tctcaacgat atctttatgg aaagtggctt ccgtaatcgt 84960 gaccgcttat tcggagttct tgatgaagtt atcaagcgca agttggaaga actcgaagaa 85020 actggcatgg gttctgacca ggatatcatg gatattctct ggaaggcgca caagatgaag 85080 atggaagaaa tgaaaatgat ggtagaactg gagaaagtga aggcagcagc tcgtactccg 85140 gctaaccaga ctaacattca gaataatatt attgctggaa gtggtgacga aaactatatg 85200 aatcttatta ccgctctagc aggaggaggt aagagataat gaaagtctct agagactata 85260 ttaacaccac agatgttatt gactttggta ttgacaaacg atttttccgt ctgccggtct 85320 ctagcattct agctcaagag gggattacac ctaatgcccc tcaggttgca attattaacg 85380 cgctggaaga tcctagacac cgtttcgtga ctgcatgtgt atcacgtcgt gtaggtaagt 85440 cattcatcgc atatacactt gggttcttaa aacttctgga acccaatgtg aaggtgctgg 85500 tagttgcacc taactactca ctggctaaca ttggttggtc acagattcgt ggtcttatta 85560 agaagtatgg attacagact gaacgtgaga acgccaaaga taaagagatt gaactagcga 85620 atggttctct ctttaagctg gcttctgcgg cgcaggctga ctccgcggtt ggtcgttcat 85680 atgacttcat catttttgac gaagcagcaa tttccgatgt tggtggtgca gctttcgata 85740 ttcagttacg tcctacccta gataagccaa actctaaagc tctatttatc tctactcctc 85800 gtgggggtaa ctggttcaaa gagttctatg agaaaggatt taacgaaacg ctacctaatt 85860 gggtatctat tcatggtaca tatcgtgata atccacgtgc tgacctgaac gatatcgaag 85920 aagcgcgtcg tactgttagt aaaaactatt tccgtcaaga atatgaggct gacttctccg 85980 tattcgaagg tcagatcttt gataccttca atgctatcga gcacgttaaa gacctcaaag 86040 gtatgcgtca cttctttaaa gatgatgagg ctttcgaaac attacttggt atcgacgttg 86100 gttatcgtga ccctacagca gttcttacta tcaagtatca ctatgatact gatgtttatt 86160 acgtactaga agagtatcag caggctgaaa agactacagc tcaacacgct gtttacatac 86220 agcactgtat agatcgttat aacgttgatc gtatatttgt agactccgct gctgctcagt 86280 tccgtcagga cttggcatat gaacatgaaa tcgcttctgc tcctgctaag aaatctgtcc 86340 tagatggtct ggcatgccta caagcactgt tccagcaagg taagatcatc gtagatgctt 86400 catgtacctc attgatccac gccctagcga actacaagtg ggacttccag gagggcgaag 86460 agaaattatc acgtgaaaaa ccacgtcatg atgcgaactc tcacctttgt gacgcactgc 86520 gttatggaat ttactcaatt tcccgtggga aataaataag tataggatgg gatactactc 86580 agttggtatc ccattcctgc attttaaaat cccctttaca aattcgacat gattatgtat 86640 actatattca tacagttgag gagaatactg tggcaggaaa tgtaaaatat aaacgtgatg 86700 ctatatcact catgcgagac ggtataaaaa gtcaatataa aaaagccgat cgctgcgaaa 86760 tctgtggttg tggtgaagaa ttagagcttc atcattacca tacagtgtct ctactagtta 86820 aaaaatttgc taaagaactc caattggatt tcactgacga agaaacagtc ctctcaaata 86880 ggacggcatt ctatgaaaga tataggcatg agctagtaga tgacactgtt accctttgtg 86940 tccaccatca ccaactatta cataaggtgt acactaaaga acctcccctt ttctctgcta 87000 ataagcagaa ggcatgggtt cttaaacaaa aggaaaagct acagaatcct caagaaaaga 87060 cacaagtcaa gactgaaact aaatcaggat tcgcaaggtt cttataatgg gtttaaaaag 87120 ctggattact gaaaagctaa atccgggtca acgtattata agagacatgg aacccgttag 87180 tcatcgcact aaccgtaagc catttaccac tggacaagcc tacagtaaga ttgagattct 87240 caaccgaact gctaacatgg ttatcgatag tgcggcggag tgttcttata ctgtcggaga 87300 taaatataat attgtcacgt acgctaatgg cgtcaaggca aagactctag acactctctt 87360 aaatgtacga cctaatccat tcatggatat aagcacattc cgtagacttg tagtcactga 87420 cctacttttt gagggttgtg catacatcta ttgggatggc acagcacttt atcatgtccc 87480 tgctgcacta atgcaagtag aggcagatgc caataagttt atcaaaaaat tcatatttaa 87540 taaccagata gactatcgcg tagatgagat tatctttata aaggataaca gttacgtgtg 87600 tggcacaaac tcccaaattt ctggacaatc tcgtgttgct acagttattg actctcttga 87660 gaagcgttct aagatgctta actttaaaga gaagttcctc gataacggca cagtgattgg 87720 tcttattctt gagacggatg aaatcctgaa caagaaattg cgtgagcgta aacaagaaga 87780 attacaactc gattataatc ctagtacggg tcagtctact gtcctgattc tagatggtgg 87840 tatgaaagca aaaccgtact cccaaatatc ctcttttaaa gatctagact tcaaggaaga 87900 tatcgaagga tttaataaat ccatctgtct tgcctttggt gttccgcaag tgctacttga 87960 tggtggtaat aatgctaaca ttcgaccaaa catcgaattg ttctattaca tgactatcat 88020 tcctatgctg aacaagctga ctagttctct tactttcttc tttggctaca aggttactcc 88080 taacactaag gaagtagctg cactgacgcc agataaagaa gctgaggcta aacatttaac 88140 ctctttggtt aataacggta tcatgactgg taacgaagct cgtgaagagc ttaaccttga 88200 acctttagat gatgagcaga tgaataagat tcgtattcct gctaacgttg ctggttctgc 88260 aacaggtgta tctggtcaag aaggtggtag accgcaaggc actgccgatg gggataaaga 88320 atgattgatt atgatggtct aaaggccatt tttggtgaaa agctgccaga atctcatatc 88380 ttctttgcta cggttgcagc acacaagttt gttccaaact acgctactct acgtaaagag 88440 tttggaatga ccactgcaca taccaaccgc aaggtttgga ataagttcaa agaagcatac 88500 gaagttactg ctccggttgt tccggctcta gctttcacga agaatctggc taaaactcta 88560 gctgttgata ctggtgcagc aattaatctg ggcgtaacag ttactggtgg tactgcacca 88620 tatacttatg cttggactaa agacggttct ccacttagtg gtgttactgg ccctaatttc 88680 aacaaagcta cagctgctgc agaagatgct ggtacttaca aagttgttgt aacggatagt 88740 aaatctactt ccattacttc taatgagtgt gtaacaacta tcaaccctgc gccggaggct 88800 taataaatga caaatgctgc tattgactat aacaagttaa aatcagcacc ggttcattta 88860 gacgcttaca ttaagtctat tgatagcgaa tccaaagagg gtgttgtaaa aatccgtgga 88920 ttcgctaata caattagtaa agatcgcgct ggtgacgtaa ttcctgcttc ggcatggaaa 88980 acgtctaatg cacttgctaa ctacatgaaa aacccgatta ttcttttcgg ccacgaccat 89040 cgtcgtccaa tcggcaagtg tattgatctt aaccctactg aaatgggtct cgaaattgaa 89100 tgcgagattt atgaaagttc tgatccagct atcttctcac taattaaaaa cggtgtactg 89160 aaaaccttca gtatcggatt ccgctgccta gacgcagaat gggatgaagc tactgacata 89220 tttattatta aagatttaga actatacgaa gtttcggtag tttctgtacc ctgtaatcag 89280 gactcaacgt tcaacctcgc taagagtatg aatggccacg attacactga atggcgcaaa 89340 tcttttactg caacaagttc taaggctgtc ccagctcaag aacgtaatct ttctgaacta 89400 gaaaaacttg cgatagcttt aggctacgtt aaagaataac ggagaattat ttaaaaatga 89460 ctattgatat taataagctg aaggaagaac ttggtctggg tgatctggct aaatctctgg 89520 aaggtctgac tgctgctcag aaagctgcgg aagctaagcg tctgcgtgaa gagcaggaag 89580 aaaaagaact ggctcgtatg aacgacctag tatctaaagc tgttggtgaa gaccgtaagc 89640 gtctggaaga agctctggat ctggttaaga acctggatga gaagtctaag aagagcgcag 89700 aactgtttgc acagactgta gaaaagcaac aggaaaccat cgttggtctt caggacgaaa 89760 tcaagtctct gttggcagct cgtgaaggtc gtcccttcgt tggtgatagc gtagctaaag 89820 ctctttatgg cactcaggat gctttcgaag atgaagttga gaaactggtt cttctgtctt 89880 atatgatgga gaaagacgta ttcgaaactg aacatggtaa ggctcacgtt aaagctgtta 89940 acggttcttc ttccgtttcc atgtctagcg aagcatacga aactatcttc tctacccgta 90000 ttatccgtga cctgcagaag gaactggtag ttggtgcact gttcgatgaa ctgccaatgt 90060 ccagcaaaat ccttactatg ctcgttgaac cagaagctgg tcgtgctact tgggtagatg 90120 cttccaaatt cggtactgac gaaactgttg gtgacgaagt taaaggtcaa ctgactgaaa 90180 tcagcttcaa aacttacaag ctggctgcta agtccttcat cactgatgaa actgaagaag 90240 atgcaatctt ctccctactg cctctgctgc gtaagcgtct gatcgaagca cacgctgttt 90300 ctatcgaaga agctttcatg tccggtaatg gtactggtca gcctaagggt ctgctgaaac 90360 tggctgctga tgatggtgct aaggttgtta ccgaagctaa agctgacggt tctgttctgg 90420 taactgctaa gactatctcc aagctgcgtc gtaaactggg ccgtcacggt ctgaaactga 90480 gcaaactggt actgatcgta tctatggatg cttactacga tctgctggaa gatgaagaat 90540 ggcaggatgt tgcccaggtt ggtaacgatg ctgttaaact gcagggtcag gttggtcgta 90600 tttacggtct gccggttgta gtttctgagt acttcccagc taaagctgct tccgctgaat 90660 tcgcagttat tgtttataaa gataacttcg taatgccgcg tcagcgtgcc gttactgttg 90720 aacgtgagcg ccaagctggc aaacagcgtg acgcatacta cgttacccag cgtgttaacc 90780 tgcaacgtta cttcgaaaac ggcgttgtat ctggtgctta cgctgcatct taatacaggc 90840 ttttcagccg ataaggagag cttcggctct ccttttttat tgggaaaaat aaatgcaaat 90900 catcactgtt gaagactaca gactatatgc taatgcaaag cgacctgata tggaaactca 90960 ggttgagatg atgattaaag ctgccaatac gctgattaca agccttctag gtatggatga 91020 tgcagatgcg gtagatcaat taatcactac aaaacctagc cgtaggaaat atttcctgag 91080 ttctccctca gccacatcta ttacaaagat gactattaac gagaatgaaa tagacccgga 91140 acagtttaaa ctgtatcctg atggtgttat tctccttaag tttaatcctc ctgaaggata 91200 tatggatgta gagtatactc aaggtggttt caatccaatg cctgaagatc ttaaactggc 91260 agcatgtctg ttagtagatc actggcataa gcaagattat cgtcaagcca agactattgg 91320 tggtgagaca gttacgttta acaatactaa gtctggtatt ccagagcata ttcgtacaat 91380 catcgaagtc tacaggagag tataatggct ctttctgatc tagctagaca aattattaaa 91440 gagcagctag atactgcagg acgatctgag aataataaga atactgttgt gtatacagtt 91500 gagacaggtt tgaaagatcc tacaagagat ggtacagttg cacaggtatc tttcaaattc 91560 tcgaaaccag tatcacaaga cttattaaat gttaggacag catctatact gaaggctgtt 91620 tcttctaact tagatctttc tggtgattta ggtgctctag agaatctaat tcaagcaacc 91680 gcaggtaaac gttccgccgt tggtaaaaaa cgttctaccg gtagagttca ggtaaacttt 91740 ggagatccta gagatactga agatggctat tctggtgcag taacaggtgc ttccggtcgt 91800 ttcgtatcga atagtaacat gcgtgctatt cttgagatag tagctaagga atacttaatc 91860 aaggatatga aaaaagcagg ggcaccattg aagttcagaa ctggtcgctt tgctaactcc 91920 ttaaagatta aagacgttat gctacgtgat gcagaaacta gtaaaggtgc tccagagctg 91980 aatgtgacgt ataactatat ggttcgtcct tactctgtgt tcaaccccgc agtttctaca 92040 tatcgaagac tttctctacg accttaccct ggcgctcgaa accctcaaaa acttattggg 92100 gaagcaatcg caaaagctgc aagagacctg attcactcta gatataaaat caaggttaat 92160 caaggaacct agtaaatgga tcacagaaca agtattgcac aagcagtggt tgaccgaatc 92220 gccacacaaa tggatggttc acaacctgac gagtatttca ataaccttta tggaaacgtt 92280 tctcgtcaaa cttataaatt cgaggaaatc cgagaatttc cttatgtggc agtccatatc 92340 ggaactgaaa ctgggcagta tcttccctca ggccaacagt ggatgttttt agaacttcca 92400 atcctcgtgt atgacaaaga gaaaacagat attcaagagc aacttgaaaa actcgtagct 92460 gacataaaaa ccgtcattga cacaggtgga aatttagaat atactgttag taaacctaat 92520 ggatcgacct tcccatgtga ggcaactgat atgagcatca cctcagtagc tacagatgag 92580 ggtctactgg ccccatatgg tttagcggaa ataaatgtaa cagtgaggta tcagccacct 92640 cgtagggcac ttcgcagata agttacagat taggagaata taaataaatg tctttacaac 92700 tattacgtaa tactcgaatc ttcgtgtcta cggttaagac tggtcacgat aagaccaaca 92760 cgcaagagat tctagttcaa gatgatattt cttggggtca ggacagtaac tcaaccgata 92820 ttactgttaa cgaagctggt ccgcgtccaa ctcgtggttc aaaacgtttc aacgactccc 92880 tgaacgcagc tgagtggagt ttctctactt acatcctgcc ttacttagat aagactacta 92940 gtaagcagat cgttccggac tacatgctgt ggcatgctct ttctagtggt aagcctatca 93000 acctagatgg cgatacaggt gctcacacta acgaaactaa cttcatggtt aactttaagg 93060 ataacgcata ccacgaactg gcaatgctgc acatttacat ccttactgat aaagcatgga 93120 gttatatcga ttcctgtcag atcaaccagg ctgaagtaaa cgttgatatc gaagatatcg 93180 gtcgtgtaac ttggtcaggt aatggtaacc agctaatccc tctggatgct gctccatttg 93240 atcctgatac tgtaggtatt gatgatgaaa cttacatgac tatccagggt tcttacatca 93300 agaacaaact gactattctc aagatcaagg atatggatac tggtaaggca tatgacattc 93360 cgatcactgg tggtagcttt acaatcaaca acaacatcac ctatctgact ccgaacgtca 93420 tgtcccgtgt taacatccca atcggttcat ttactggtgc attcgaatta actggttctc 93480 taactgctta tcttaacgat aaagctctag gttctatgga tctgtacaaa gatctaatca 93540 agaccctgaa agtagttaac cgtttcgaaa tcgctctagt acttggcggt gaatatgatg 93600 atgagcgccc agccgctgtt ctagttgcta agcaagctca cgttaacatc ccaactattg 93660 agactgacga tgtacttggt acttctgtag agttcaaagc aattcctact gatctggata 93720 ctggtgatga aggttatctt ggtttctcta ataagtacac caaaactact attgcaaatc 93780 tgattgcaac tggtgacggt ggtaaagcag ctccggttaa ggtaacaggt attactgtta 93840 aatccgcggg tgatgcagct tccgttgata atagtgcaac tcttcagatg actgtagatg 93900 ttttgcctgc tgaagctact aataaggaag taacttggtc tatttcctcc ggtactgctg 93960 ctactattga tgcaacttct ggtttactga ccgctgatgc ttctaagact ggtgcagtta 94020 ctgtgaaagc cgtagctaaa gatggttctg gtgtagaagg tactaagagc attacagtta 94080 ctgcttctgg tggctaatta aatgtactac tctctaatga gggagtcaaa agttatagtt 94140 gagtatgatg gcagggctta tcattttgat gccctgtcaa attacgatat caatacgtcc 94200 tatgaggaat ttaaaactct tcgtaggact atccatcgta gaaccaacta tgctgactct 94260 attattaatg cacaaactcc atcttcaata tcgctggcta taaattttag taataccctt 94320 actgaagcta acttctttga atggatgggc tttgaccgag agggtaacac attcttacta 94380 ccactttata gtacaaatat tgagccaaca atgtttaata tatacatagt taataaagat 94440 aataactgtg tgtactttga gaattgctat gtatctactg tagacttttc tctagacaaa 94500 agcgttccta tcctaaacgt aggaattgaa tctggtaagt tctctgaggt gtctactttt 94560 actcctgctg gttctattgt tcagggtgaa gttatgtcat atagtcctcc tagagtatct 94620 accaacagta atgttttacc agctctttta tccgcttcta tgtcattcca gcaacaatgt 94680 tcttggagag aagataaaag cgtatttgac ataaacaaaa tatataataa taagagagct 94740 tatgttaatg aaatgaatgc ttcggcaaca attgcatttt actacactaa gcgatttgct 94800 ggtgatatgt tttataatat tgaacccgaa actgacatac cattaaccat ccgaaatgaa 94860 catatttcga ttgactttcc ttcagcacgt ataacaaaac gcctacaatt ctcggatata 94920 tacagagttg aatgggatgt aatccctact gcagactctg atccggtaag aatcgacttc 94980 tttggagaaa taaagaaatg atcaatttaa aagatattac ccttgaaact cgtactattg 95040 agctggctta cccaggtatg cctaacttca agctgcaact gaactacatg tctcgtgctg 95100 catctaagcg tgttatctct tccgctaagc gtgatgagtg ggttaacggt actatgattc 95160 aggttcagga cgatgataag tttattgaag ctttcgttga tactgcaatt gctggctgga 95220 ctggcttgac gattggagat gttgagaagc tgatgcttat cgaaactgaa gctgatccta 95280 ctactgaagt tccgtttagt cgcgataacg ctatcatgct tatgcagaac tctgctgcgt 95340 ttgattcttg gatcaaccag acagtgttcc acttagacac ttttcgtagc tcaaaagcgt 95400 aagcagttac tagatgctgt tgccgatttt gcagataaat gcattaaatc ctcagcatct 95460 aaaatgacaa aacaacaata tttaatgctt tgcgagtctc taggtattga acctgatcct 95520 aaggcaatgc ctgttgaact cgaagatttt ccacctattg tatctactag tatgaatatt 95580 tataatagtc ttattgactg tttcatacca ggtgactttc ctatctttat aggaaaagac 95640 aaaagtgcat taggtgtttt atttgatatc tatgggatta ctgatcctat agagaaagag 95700 tttgttctac acatcatcaa tatatttgat tcgaaagctg tagatgcagc tcgtaagaga 95760 gccgagaagc atagtaaacc taatggtcgt accccgaaca ttaaaccaca tgctagatct 95820 cgagctcagt aaaagtttcc tccaatgggc gttccacgat gaggcttggc tctgggtgat 95880 ttgcccagag ccttttttat tgggaaaaat aaatgactga taagctaata cgagaactac 95940 ttatagatgt aaagcagaaa ggggcaaccc gtactgcaaa atctattgag aacgtttccg 96000 atgcgttaga aaatgctgct gccgcttccg aactgactaa tgagcagtta ggaaaaatgc 96060 ccaaaactct ttactccatt gagagggcgg cggatagagc agctaaaagt ctaactaaaa 96120 tgcaggctag tagaggcatg actggtatca ccaaatctat ggatagtatt ggtgctaagt 96180 tagatgacct tgctattgct atgatcgagg tatccgataa gctagagtct ggatttacac 96240 acgttggtaa atctgttaag gcgatgggta acgatgtagc tgctgcaact gaaaaagttc 96300 aagatagatt atatgatact aaccgagcac taggtaatac tgctagaggc tttaatgata 96360 caactaccgc tgcaggacgt gctagtcgtg caattggtaa tacttctggt tcagcacgtg 96420 gtgcaactcg tgactttgca gctatggcta aagtgggtgg tggtttacct cttatgtacg 96480 ctgctatcgc atctaacgtg ttcgttctac aatctgcatt cgaacaactt aagatgggtg 96540 accagttaaa ccgtcttgag aagtttggta ctattgttgg tactcaaaca ggtactccgg 96600 tacaaactct agctagatct ctacaagagg ctgctggtta tgcaatctcc ttcgaagaag 96660 caatgagaca agcatcctct gcttctgcat atggattcga tgctgaacaa cttaataaat 96720 ttggtctagt agctcgtcgt gctgctgctg ttcttggtgt tgatatgact gatgcactta 96780 accgtgtaat caagggtgta tctaaacaag aaatcgaact tctggacgaa cttggtgtta 96840 ctattcgtct taatgatgct tacgctgact acgttaaaca gttaaatgct gctaacacag 96900 gtattacgta taacgtcaat agtctttcca ctttccaaaa gcaacaagca tatgctaacg 96960 cagtaatcgc tgaatctact aaacgtttcg gctacttgga tgacgtactt cgtgcaactc 97020 catgggaaca gtttgctgct aatgctgatg ctgccctaag aaaggttcaa caggctgctg 97080 ctaagtattt agggccggta attgattcta tcaacgcagt attctatact tctcaagcat 97140 ctatctcggc ttctgctgct agagcacagg aagaaactaa ccgtcagatt gacccaacta 97200 atgttggtgc tgtagcttta agtctttccg cttctgagga aggatataat aaagctctgg 97260 atatgtataa agaatcccta gaaaagcgta ataagttaaa agctgatcta gacaagcgta 97320 tggaacaggc tgacttcttt accgcaggtg caattcgttc tgtagctcaa ggtgtccctg 97380 tgagcgctgc tgctcttggc gcttcagagg aaaacaagaa gttcgtagct gaaactgcgg 97440 ctatggcact acaagtagaa cgtttggata aggaagttac tgattcaacc gataacttag 97500 gtgcatggaa atctgcatat caagctgctg gtgctgcggc tgctaaggct aatccagaat 97560 tccagaagca gattaatctg cagaaggata tgaatgaccc tgatgcggtt tatgacttta 97620 actctgcaac tctgaaagga ctgacagaac agcagaaagc atatgatcag gctaagaaaa 97680 ctgctagtga tttagctaac gatatccaga acatcgctca gaatactaat actgcggcta 97740 aaactagtgc atccctatca gatacgatta agactattga gtctctatct gctggtactg 97800 gtaaaaatgc tgatgagtat gttaagagcc tgaacttagg gtataatact cttagtgaga 97860 tgaagactgc ttcacaagct cttgccggtt acgtaaaatt aactggtaat gagactaaga 97920 atcagttaga agttcagcag aagattgccg aagtgtacaa ccagactaag gacaaagaga 97980 aagctcagga agctggtaga cgtttggaaa ttcaacagtt agaagaacag gaagcagctc 98040 ttaagcgtgt cctagaaact aacaaaggta acaaagcaat cgaaggtgaa atagctaaga 98100 ttcagttaga acgtatcaag attactaacc agggtatgga agctcagaag aaggttaagg 98160 actataccga taagattctt ggtgttgatc gtgaaattgc tctcctgaat aaccgtacta 98220 tgacagatac tcaatatcgt ttagctcagt tgaaccttga actgactgtc gagaaagaaa 98280 agtatgaatg gtactccaaa cagattgata agcataagga agctgaacaa tcaagacgtg 98340 ctcaggcaca aattgaacgt gaaatctgga aattccgtca ggatcagcaa agggaaatgg 98400 ctgctggcag ggaggaggag caacaaagga agtttaccgc aaatccttta atgggagctg 98460 cggaacgtat caaggagcaa atccagctat tcgaagacct taagcaaaaa actttaggga 98520 atgctgctgc tcagttagag tataataaaa agctagcaga aactagagcc cagctcgcag 98580 ctttaagagt acagcgcgat gcagaaatgc agtcttctgt aggtgcggct gtaggggcta 98640 cctatactcc tactactgga ttaactggag aagataaaaa cttcgcagat atgcagaata 98700 gaatggcgtc ctatgaccag gctatttcta agctgtctga actgaactcc gaagcaactg 98760 ctgtagcaca aagtatgggt aacctaacta atgctatgat ccagttctct caaggatctc 98820 tggatactac ctctctagta gcggctggta tgcaaactgt atcttcaatg attcagtata 98880 gtgttggtca acaggtaagt gctattgatg cagctattgc agcagaacag aaacgtgatg 98940 gtaaatctga gcaatctaaa gctaagatca agaagttaga ggctgaaaag ctcaagatcc 99000 agcaagatgc agctaagaag caaatcatta tccagacggc agtagcggtg atgcaggcag 99060 caacagctgt tccgtatcca ttctctattc ctctgatggt tgcggctggt ttagcaggtg 99120 ctatggctct tgctcaagca tcttctgcat ctggtatgtc ttctattggg gactctggtg 99180 ctgagacagc tggttactta actcttggag aacgccagaa gaatgtagac gtttctatgt 99240 cggctaatgc aggtgaactt tcttatattc gaggagatca agggattggt aatgctaact 99300 ctttcgtacc tcgtgcagaa ggtggtaata tgtatcctgg agttagttac cagatgggtg 99360 agcatggtac cgaagtaatc actcctatgg ttccgatgaa ggctacccct actgatgaac 99420 tcaaaggatc ttcaaaaggc acatcgggta gacctattgt gctgaacatc agtacgatgg 99480 atgcggctag cttccgagat tttgcttcga gcaatagtgc tgctttcagg gatgccgtgg 99540 aacaggctct taatgagaat ggaaccaccc tgaaatcact tggtaattct taatactgga 99600 ggaggacttt atgtcctcct tttctttatg gaaaaataaa aatttcttga taaaattttc 99660 caatcctatt ataatattgt tattaagagg agaaattaac tatgagacta ccagacccat 99720 acacgaaccc agagctatca gggttaggtt ttgaaagcgt taacttagta gacaatgacc 99780 ctatgattcg tgatgaatta cctaatggta aagttaggga ggttaaggtg tctgctcaat 99840 attggggtat taatatctct tatccagaat tattcccaga tgaatttgct aaattagatt 99900 cattcattct agattacaaa agaacaggta gctatattga tgtgttatta ccgcaatatg 99960 aagctttccg agttcgtggt gatactagtg ctgtaaatat tcctgctgga caaaaaggtt 100020 caacagttat tatggacact aaaggtacac ttcgtggact gcctaaacca ggcgatttat 100080 ttaaattgtc caaccaccca aaagtgtaca aaatcacttc tatcaacaca gtaggaaata 100140 cttggaatat aagtgtttac cctgatctat tcattactac tacgggtagt gagaaaccgg 100200 tatttaatgg tattctattt agaactaaat taatgaacgg agacagcttc ggctctacgt 100260 taaatagtaa tggtacatat actggtattt cactttcctt gagggaaagt ctatgaagaa 100320 aattcttgac agtgcaagag agtatttaag aactaataat aaaataaaaa ctgcatgcct 100380 tattactctt gaattgccaa gctctaccgg atcaagttct gcatacattt atcttacaga 100440 ctattttagg gatgtaattt ataatggtat tctttacaca tccggtaaag ttaagtctat 100500 aacaacacat aaacaaaata gaaaactttc cattggtagc ctttctttta ctatcacagg 100560 tactgctgag gatgaagtat taaaactagt ccaaaatggt gtatccttcc tagatagatc 100620 tatctcaatc catcaagcgg ttattgatga agaaggtaac attcttcctg tagatccaga 100680 cactaatggt cctttacttt acttccgtgg taaaatcact ggtgggggta ttaaggataa 100740 cattggtact tcgggagtta gtacatccgt tattacatgg aactgttcaa accaattcta 100800 tgactttgat agggtaaatg gacgtttcac agatgatgct gaccatagag ggcttgagat 100860 tgttgccgga caattagttc cgtctaatgg tgctaagcga cctgaatatc aagaggacta 100920 cggattcttt cacgctaaca agagtatttc tattcttgct aagtatcagg ttcaggaaga 100980 acgttataag ttacaatcta agaaaaagtt atttggtctt tctagaagct atagcctaaa 101040 gaagtactat gagactgtaa ctaaggaagt cgatcttgat tttaacttag cagctaaatt 101100 cctaccagta gtatatggag ttcagaaaat tcctggaatc ccagtatttg ccgatactga 101160 actgcataac cctaatatcg tatatgtagt gtacgccttc tgtgagggtg agattgatgg 101220 attcctagat ttctactttg gtgatgtacc aatgatctgt attgatccta atgatagtaa 101280 gtctcgtact tgctttggta ctaagaaagt agctggtgat accatgcaac gtattgcttc 101340 tggtcaacca agctctgaac cttctgttca tggacaggaa tataagtata atgatggtaa 101400 tggcgatatc cgtatctgga cttatcatgg taaggctgat caatctgctg ctgatgttct 101460 agttaacatt gcaaaagaga aaggtttcta tctacagaac atgaatgaga atggaccgga 101520 atactgggac tctcgttata aactgttaga tactgcttat gctattgttc gctttactat 101580 taatgaaaac agaacagaaa ttccagaagt tagcgctgaa gttcaaggta agaagattaa 101640 gatctatcat tcagatggta gagtaactgc tgataagact agcttaaatg gtatctggca 101700 aactctggac tacctaacct ctgaccgcta cggtgctaat atcactattg atcagttccc 101760 tctccagcaa cttattcaag aagcagctat cctggatatc attgatgaat cctatcaggt 101820 ttcttggcaa ccctactgga gatacgttgg atggactgat gctgtaggag agaatagaca 101880 aatagttcaa atgaatacta ttctcgatac ctctgagtca gtatttaaaa acgtgcaggg 101940 attaatagag tcttatggag gtgctatcaa caacttatct ggtcagtata gaataactgt 102000 tgagaagttc tcgaatactc cactggagat tgacttctta gatacctatg gtgatctgga 102060 gctatcagat actactggta gaaataagtt taactcagta caggcctcta tactagatcc 102120 cgcattaagc tggaaaacta actccattac attctttaac tctatattta aggagcaaga 102180 taaagggtta gataaaaaac ttcagctttc ttttgcaaac atcactaact actatactgc 102240 gcgtagcttt gcagatagag aactgaaaaa gtctcgttac tcacgaactc tcacattctc 102300 attaccatat catttcatag gcattgagcc taacgatccg attgctttta catacggtcg 102360 ttatggttgg gacaagaaat acttcctagt tgatgaagtt gaaaactcta gagaaggtaa 102420 gattaatatt acactgcaag agtacggtga ggatgtattc attaactccg agcaggttga 102480 taatagtggt aacgatatcc ctgatgttag taataacgta ctgcctccta gagactttat 102540 gtatacacca acaccgggtg gacaagtagg atctattggt aagaatggtg agctatcttg 102600 gcttcctagc ttaactaata acgttgttta ttactccatc gttcactctg ggcatgctga 102660 tccttatata gtacagcaac tagaaactaa tccaaatcta cgaatgatcc aagaaattat 102720 tggagaacct gctggtttag cagtctttga gattagagct gtagatatta atggtagacg 102780 aagttctcct gtaacattat cggtagaact taactctgct aaaaacttaa gcgtcgttag 102840 taatttcagg gttactaaca cagcttcagg agatgcatcg gagtttgtag gaccagacgt 102900 taagttggcc tgggatagaa ttccagaaga agatattatt gatggaatct tctacactct 102960 cgaaatctac gataacctag atcgtttatt aagaagtgta cgaattgaag atcagtatgt 103020 ctacgattat ctactgatat acaataaggc agactatgct cttcataacg aggatgctct 103080 aggtattaat aggaagttac gcttccgtat aagagcagaa ggtgataatg gcgagcaatc 103140 tgtggattgg gcatctattt aaatgatttc gaataatgca ccagccaaga tggtcttaaa 103200 tagtattatg actggatata ctatggcgta tatccagcat tctatctata ccgactacga 103260 tgttatcggt agatcattct ggcttaaact tggcgaagaa gtggatagaa gagatttcac 103320 tggaatcgac actttctttg ttatgatcaa taacttaact ccctcaacta cctatcaggt 103380 tcagggagct ttttatgact caattattga ctcagaactt ttaaatgcaa aaattggtat 103440 taacctctct aatgaaacta actttaaaac aaaagagaag ccaataattg ttgcagcaag 103500 atctgagtca gaacctgtgg atgttggggt gggcgcacca atagttgttg tggaaacaac 103560 tggtgaagca agctactgta ctattgagtt aaaaagtaca gccactgaag acagtccatg 103620 actaaatatt atattggggc tttaggttct actattaaat ttggtggagt tcctatcgga 103680 gattataaga tcagaatatc tggtcaagta actatgcctg atggtgttac agttgattct 103740 tctgggtact atgagttccc taatattcta actgtagctt ataattttgt tcctcctact 103800 gcacctatcg atattgtttt taaagctgca cgaattgctg atggtaaaga acgatatgat 103860 gttagaattg agtgggactg ggaacgtggt gctggtgcta acgttcgtga attcttggtt 103920 acttacataa actccgagga atatgctaaa accggatggg ctaaagctca aaagataaac 103980 gtgggtgctg ccagagctgc aacaattata tcattcccat ggaaagttga acatactttt 104040 aaggtatcat caattgcctg gggaccaaat aagcaagata taacagaatc agctcctgta 104100 acatttatat tgaatgaaga tactcctcta gacaacagct ttgtcaatga gacgggtatt 104160 gatgttaact acgcctttat taagggcagc atgaaagatg gggaaatctg gagacagaca 104220 ttcttaattg atgcagctac tggtgctatt aacattggtc tgctcgatga agaaggaaaa 104280 acacctattt ctttcgaccc tataaaccgt gttgttaacg ttgatggtaa agtaattact 104340 agagatatta atgctgcgaa ctttatcatg actaacttat caggtaaaga taacccagca 104400 atttatactc aaggtaaatc ttggggagat aataactctg gtatttggat gggtatggat 104460 aatacctctg ccaaagctaa actagacatt ggtaatgcta cacaatggat acgttatgat 104520 ggtactactc tgcgtatttc tagtggtgta gtaattggaa caccaaatgg tgacgtagat 104580 attggaactg gtttacaggg taagcaaaca gtatttgttt ataagttagc aacatcttta 104640 ccggccaaac cgctagaaca agattatcca cctcctggtt ggtcaaaaac tccacctaac 104700 cgtacagata tgacacaaaa tatctatgcg actacaggta cacttgatcc agttactaac 104760 aaacttcttg aaggtactag ctggtctgat gtagttcagt ggagtggtac tgaaggtact 104820 ataggacatg atggacagcg tggtcctggg atgtactcca tgggtattcc tggattaggt 104880 ggttgggatg atggacaggc taacgcattc ttccaaaata actttggaaa acccccggtt 104940 aagtatgatg ttctaacaca atttaacagt aatgctccac aaacagcatt tacccgccaa 105000 tggaacgggg ctggatggat taaccctgca atggttcttc atggaaacat gattgtcaat 105060 ggaactgtaa ctgcggataa gattgtggca ggaaatgcct tcttatcaca aatcggtgtt 105120 aatataatct acgatagaaa tgctgcgtta tcagggaacc ctgaagcata ctacaagatg 105180 aagatagacc taaatagtgg gtatatccat ataaggtaat tataatgagt acagaaaacc 105240 gagttattga tattgttgtt gatgagaaag taccttatgg tcttatcatg cagtttatgg 105300 atgttgatga cagtgtatat ccaccaacag aagttcctgt taacttaacc ggatattcac 105360 tccgaggaac tattaaatct agtttagatg agaacgccga ggttctagct aactttaaaa 105420 ctcgtgttat tgatgctgct caaggtgctg ctgcaattag tctttccgtt gcagacgtta 105480 ctaatattgg tgaaaaagca tctaaagaac gtgataagta taacccaaga caacgttttg 105540 ctggttatta tgatatccta atgactcgtg atgtaattgg ctctgaagtc agttctttcc 105600 gtattatgga aggtaaagta tacatcagcg atggagtaac tcaataatgg cacttaaaac 105660 taaaattatt gtacagcaga ttctgaacat agatgacact acaactactg ctagtaagta 105720 tcctaaatat acagtagttt taggtaattc tattagttct attactgctg gtgaattaac 105780 tgccgctgta gaagcctctg ctgcttctgc tgcggcagca aaagattccg aaattgcagc 105840 aaaagaatct gaaacaaatg ctaaggactc ggagaaccta gctgcaattt atgctacttc 105900 ttctgaaaca tctgcaactc aatctgcggc atctgccgcg gaagctgaga gacaagctgg 105960 tctgtcacaa aagagtgctg aagcatccgc tacatcggcg gaagaatcta aaggattcag 106020 gaattctgct gaattagctg ctcaaaatgc ggagaccagt cgtagacttg ccgaacaagc 106080 taaaactgcg gctcaacaag ctcagacagc tgcagaaact gctaagactg gtgctgaaac 106140 agcaaaagca ggtgcagaag ctgctgctac aactgctgga gaacatgctg cttccgctaa 106200 acaatcagaa ttaaatgcta aagaatcaga aactaatgcg gctggttctg ctgtagaagc 106260 aggagataag gctgtagatg ctactactga ggcaaatcgt gctaaggctg aagccgatcg 106320 cgcagctcag attgtagata gcaagctgga taaagaagat atatctggtt tcatcaaagt 106380 ttataagaca aaagcagaag ctgatgcaga tgttgcaaat cgtgttctgg gtgaaaagat 106440 tctagtctgg aaccaaactg attcaaaata tggttggtat aaggttgctg gtactgctga 106500 ggctcctgtc ctagaattag tagaaattga acagaaactc gtatcaatca ataacgttca 106560 tgcagatgat gctggtaacg tacagatcac tcttccagga ggtaaccctt ctttatggtt 106620 aggtgaagtt acttggttcc cttacgataa agattcgggt gttggatatc ctggtgttct 106680 gccagctgat ggtcgtgaag tacttcgtgt tgactatcct gatacttggg aagccatcga 106740 agctggtcta attccttctg tttcagaagc tgaatggcag gccggtgcaa ctctctactt 106800 ctccactggt gatggttcca caaccttccg tttaccagat atgatgcaag ggcaggcttt 106860 tcgtgcacct actaagggag aagaagacgg cggtgctatt aaggaacaga ttccttatat 106920 cactactgtg aatggaattg gtcctgctga tgatactgga gctattaagc ttccttacgt 106980 ggcaatggtc aatggaacta ttcgtccaga tgagaacgga aatctagcac taggtaacgt 107040 agtaaccaaa aacgtttgga acggtactga tggagaagtc ttactaagag gtgcttttgg 107100 tcttggtggt gctggtttaa tccttaatga gcctgatgct gtttccttct ttaaagcaat 107160 gcgtgctttc ggttcagggt attatagaaa tgataacgag agtaacttag taatccctaa 107220 gtattctgct ggattctact ctaaagttgg agatacccat acctttattt gttctgccta 107280 tagcaatggt gttgcttttg tagcttctgc ttctgataga gatttagatg aagaatctac 107340 agtacatact aatatcctat acggtactgc aaataagcct gatttaaata atgatactca 107400 aggtgtctta ggtctcgata aaggaggaac aggagcttct acagttagtt ctgccaaaac 107460 taacctagaa gtggatagaa taaagcagct agtaggagga acacatataa cctctcagga 107520 tcaaaatata gtgtttatgg ttcaggacac taaaaactgg ggggtatacg accattctga 107580 aaacaagtgg atatctttac ctgttgaaca cggtggtact ggagctactg agccagttca 107640 agctagaaaa aatttagggg ctgcaagtgc cggtattaac tccgatatta ctcagctacg 107700 taatatggag ggttggcctc ttagcattaa gaacggtggt attattactc gtaaatacca 107760 cgcagttcct tcggatgggt tttacttagg atcggaagtt ttttctgctc aggttcaact 107820 ggataattca gagaatccag atacccctcg tttagaggca ttattttacg ctgaaggtaa 107880 ctatgcccaa tctcgtactg aaagggctac agtagctgct tataggcgta ctgcagatgg 107940 ttcgttaact gctactaaat atgctaacct gtatatggat tcaggggctt ggaatgcaga 108000 acgtatgcaa gctctagggt attctaaagg ttgggacgat gattcctttg gtttcctcgc 108060 tccttttcaa gcttctgatg taacgggcaa taataatgga ttcgtaccta ttattagtgc 108120 tatgactcaa tccacaggtg gctacccaat gagggctact actggattaa tatctcgagg 108180 tactagtact tggcctgcct atgtatttag acttcgtgga gattctaatt ggggatgtac 108240 ctatcagttc cacatgacag gggatattga cggatggggg tcagactaca ataacgttgt 108300 ctttaacttt acttacacaa aaaatgcggt gtcggatatc aatctaaaag ataatattca 108360 agacgtatcg ggtgaagaat ctcttgaaaa cattgaaaaa atggagttca agaaattcac 108420 ctataagttt gataagaaaa agagtattag acgtggtgtt attgcacaac aaatagagtt 108480 gattgatcct caatatgtta aagctattgg caatcctgaa accgacgata ttactttaac 108540 cctagatacc aatccattgt taatggatgc tttagctgct attaaggtat tatctgaaag 108600 aaataagtct cttgagacta aattacaaga gatgtctact gttattgaaa acattaacga 108660 aaagcttaat ttgatgacta gattatcaaa tatagaagca gagttagata aaatgaaagg 108720 tactagttaa tgtcacgtaa tttaatgcca aaaagtgggg ctatggcccc ctatgttgta 108780 gttaatagag acgccgcagt tgccggcgtt ttctctgttg atggagaagc tggtgctgtt 108840 gtgctaactt ccaaatacct gcaaatctct aagtatacta ctgataaagc tgctactgat 108900 gcagctatca ataatattaa cgagtcgatt ggtaatatta atactgcatt aggaggtgtt 108960 aatactactt tagatactaa agcttctaaa ggtgccaata acgatattac agaattaaat 109020 gcacttacta aagctattaa aatttctcaa ggtggtactg gggcagtaac gctagaaaat 109080 gctagagcaa atctacaagt agaacgccta cgtcagcaag atagcggaac ttttattacc 109140 tctcctgacg gtaaatattc tttatttatc tataatagtg gagattttgg tttaattaat 109200 agtgctagtg cagcagttag tgctatgaaa gttgctttcg gtggtacggg tggaactact 109260 cctaaaactg caagaaaagg ccttaatgtc cctgttggtg ccttggctga gatagtacct 109320 gatggagata acgtattgaa ctatgtagct gttgctggcc agagtgggta ttattcctct 109380 ggtgacgcaa tagttaacgg tcctcctaag caagagggtt ggtggacgta taacttccac 109440 tgtcatggag tagacataaa tggtgtggca caatatggta tacttcgtgc agttggattg 109500 tctggtagtt cctgggttaa cgttcttgat ggtactgata actggagagg atggcaggaa 109560 caatttaatg ttcaaactac tgttcaaatc tctaatgggg gtacaggtgc aacaaacata 109620 agccaagcaa gacgtaattt tggaattggt gaaactgata ttcctgtttt taggggtgtg 109680 aatcttattg aaaagaatgg tgctaattcc ggcattcttt acctaataaa caagaacgca 109740 gaaggtgtgc aactttcata ttccaggatc tacaatgaaa ttcaaggagc gaccgctaaa 109800 gcaacaatac aagtaacaag agaaggtggt gataataact attatcaatt tgacgagcat 109860 ggtaatgcac taaactataa ctctataaca gtaggtcgtg gtattggtaa cgctcttggt 109920 gataattcat tggttattgg tgatactgac actggcttta aatggggtgg tgatggtatt 109980 attcaaattc attgcaatgg tagtaatatt gcatcttatg aagcgcacaa tatgcatatt 110040 aatggattac ttacaatatg gcctactgag aataacgcaa atggggttag agtttcaggc 110100 gctagaactg gtggcggtaa tgcgttaatt gggggtcaaa ttgctggcgg cggctgggat 110160 ggttggcgag atcgcgcatc tggtctactt gttgaactac caggtgatga tgccgcatca 110220 aacatattca aggctgttag gtggggcggt gattgggctg ctggtcttga tgttgtaaga 110280 tatggtgctg gaggttgtga ggcgcatctt aatataagag gtgcaactta cacatttaat 110340 gatgctgggt atgcgtctgc tgttcagtgg gttaacacat ccgatattcg cctgaaagca 110400 aatctaaaag agattgaaag cgctaaagaa aaagtgaaat cgattaaggg ttatacttat 110460 tttaagcgta ataatcttga tgaagatgaa cactctattt attctgagga agcgggtgta 110520 atagctcagg atgtgcaggc tgttttgccg gaggctgttt ataaaatctc taacagtgag 110580 tatttaggtg taagttatag tggtattacc gctctcctag ttaacgcttt taatgaacta 110640 atgaagttgt tgaaaagcag cagcaagaaa ttgatgaact taagaaattg gtaaaacaat 110700 tacttgataa gtaaaagaaa accccagtgg atttccactg gggtttatta ttatctgctg 110760 ccagcaataa cacctaagtt aaacactgct aacatagctc taagagtagg atcttctgaa 110820 ttgctacata gttcgacaaa agcctcttca tcccactgga ttacattgcc ttcacgatct 110880 accttaagaa ctacgttatt cacatccggt tctgctacca taacatgatg atacgtagcg 110940 ggatgttcaa atgatcctat catattagga tcagcaaaat tatttacttc ctgagcttcc 111000 aaagccgcct tctccacgat cagtctcctc tagttcgtca acgatttcaa aatgatgagt 111060 tgagtagtgt ggtagtacta ctagctgaca aagtctctcg aaattctcca gagtttgcat 111120 ttcagaaccg tagttaaaca ggttcatctt aatagtacca cggtagtctg agtcgatcac 111180 tcctgcggta tttgcgatca ttagtttacg cttacctaaa gagctgcgag gaactaccaa 111240 accaaaccaa cctcgcggaa tttccaccgc gacaccggtg tcaatcataa gggatttgcc 111300 tggtgcaata gcacgtaaat ctgccgcaag gttagtacca aagaatgcac gcagatccat 111360 acctgcggca tcatcagaac caatcctcgg catgcaatct ggatgagtta gtttaatttt 111420 aatcattgtc ctgcgatctc caaaatatct ttcgtaaact tatctaatac gtcttgaccc 111480 acagcagcaa tagcatctac acaataggtc ggtaaatcaa ccagaatcag atttcggaaa 111540 agcaactcct ccgatgcgtt cagattctgt atatatttct gtttcccagg cagagggagt 111600 tgatcaataa tatccagaac attaccaaat tcacggataa tattataacc acgttttgca 111660 ccgatgcctt caacaccacg gatgttatca ccaagatctc ccataattgc tttcagagag 111720 ataaactgat ctacatcgtc aacgttatga tgttcataca tatcacgaag atggtactca 111780 cgacgtgtgg tgaaggagaa gcgagaaact cgatcagtta atagagtatc ccagtcacca 111840 tcagtagaga tcagccaaac gtgatcatat aaatgaccaa tgagcttaac aatataagct 111900 gccatatcgt ctgcttctac accacgaata gtgaaagttg ggaatgtggt ttcacacaac 111960 tcgaaagcat cacgaagata ctcgaagaac tgttcatcta gtgctttctc ttcttctgta 112020 cgttgagcat acttttcatc acggttgccc ttataatcag gaaggtgctc taaacggaat 112080 acagactttc ctttatcacc gagaacgatt gtagttctag ctgagtagga ttttgcgaga 112140 gattgaatag tagaaacata acttgaagcg aagggtttct tactattatt atgtttgaag 112200 cggaagccta agttagttcc atcaacaatc attaggttgc gacgagaagc cagctcagct 112260 tcttcctctt caataaattt tccccaggat ttactcatta tttaattaag tcctcaacag 112320 atgcgtgatg taaccacggt tcgaataacc cgattacgat ttccatgtct ttcttattta 112380 acaccatatg ggtacgactc attaagttgt cgaccatagg gtctgagcta tccaaagcaa 112440 ttaaccactg tcctctgtct ttcttgaata ttaaggctgg tttggagttc atctgttcac 112500 cttcacggga acactgctgc caccacttct ctagagtgga ttcaccaacg ttaaataaat 112560 tacttgatat gttatcatct ttataccact taacttcgaa gcaatattta ctaatgtgtc 112620 cgctttgtgg tggaaggtag atgtcaccct tcagtccatg gctctggcca aaagcaccag 112680 aacctggaac acgttcccac tcaagacctg tacgttcacg tagtatatct cttacctgat 112740 attcaccacg tttacctttc tctctactat ctacggccat gttttattct aagtaggaaa 112800 atccttctgc atcttttttg acagtaatct tatgggctaa tggatgcgta tgcccatgag 112860 aaacaatgat agaattcaga ctatcttcct catttaataa ttcaacgaga gtatcaagtc 112920 ctttcgtatc aataaagcta ataacttcgt caaggaacag aagattaata ttaactttac 112980 taatagatgt taacagcatt cgaatagcta acagagttgc taggttaatt ctactctgct 113040 gaccagtaga gcagttttcc atactggtgc ggtttccatc attgaagatt actacttgta 113100 atttagtttc atcaagttca aatccaagtg cgaacttacc accagtcata atagaaagat 113160 atttattaat caattcctca aatactttca cactatgctc tagtttatat cctaccagat 113220 ttttcaatgc agcgatcaga atatcaagat cagcaacagc ttctgatacc ccatccagtt 113280 tggacgttat ctcagacatt tctgcctcag ctttttcaat ctgttctagt ttcgctttat 113340 attttgcatt ggctaattcg acatttgcat tatgctcttt ggcaactgca actttagaac 113400 gtccatctgc gatttcctgt tctaattgtc ggatttgcac ctgtaggatt tgcacattga 113460 gttcttcaaa agaagcatca ctcattgaat ttttgaactc gtctctagct gctactgcct 113520 tatccaaagc atccttagct ctagtatatg cagtgtactc gacttgttct ttcttcaact 113580 gctctagtgt agcttcaaga gattgtttct ctttaaacag aggatcatac tctgttctag 113640 ccatatccat tgctttttga gcagcagttg tatcaaggtg agtaccacaa gtaggacatt 113700 cagtatttga agcctcttgc ttgaacttct gataacgttt cttgacttct cccgcacgtg 113760 aggtaacaat cgttaggtca cgcgtaacac ttgagatctc ttcagattgg tccgtgggcg 113820 cagggaaatt tttgaaaggc tcgaaagatt gttcggcaac ttgtacagct ttgtccaaat 113880 tacggagttt agtaatatta gcctcttgtg acttggctaa tgccgcctta attttcgatt 113940 cagtaagttc ttgtgctaac ggttcttcat caaattctgg aacttcaact ggctcttgta 114000 aacttccgag attgcctttt ccattgagga tcttcgtaat tacggccatc tggccttgca 114060 agttacttaa cgtacttgcc acttctttac gatcagcctt aatagtttct gacatctctt 114120 tatactgttc ctgattgaac agattaacaa gaaaagcttt acgtgtcgca tcagttgctt 114180 tgaggaagtc tagattggaa ccgacagatt gatagatcag tttagtgaat gtttgaaagt 114240 caccacccat aatctcttca atcatcttat acgtttgagt tgctgtgtgt ccactgatat 114300 cttctccatt cttaatcagt gtaaccttag cagttgattt aactacttta tgcaactcat 114360 actcatcttc atctttcgag aagtaagcgt gcatatcgta ctctttcttc ggagaacccc 114420 aagagaatag ggcgtccttc ttgataccac gtgagttctt gttatagaac aattcctcga 114480 taacagtagc aatagtggac ttccctagac cgtttcctcc gattaactga gtaactggat 114540 tcttatcgaa atgaattacg atatccttac cgtaagacat aacgttgctg aattttagtg 114600 tcttaattat aatctttgac atatttagca gctctagcca agattctatc aatgtcgtct 114660 tgagacagct tttcaacttc acggaagtaa agctcaagct cgcctaacat atcaagatca 114720 acaagattta acttagcatc tttagtaact cgatggttaa tctttttatc taagagatcg 114780 gaatctttaa tagactttaa ctgaacgacg tcaccagtga cttcataaat tacacggtca 114840 tagtcactag gctccatttc ctcaccaact ccgattgttt tacgaatcag ttgtggtaaa 114900 tcaccaagtt caatccattc tacttttaat gtgtcggtat caatgataaa acaaccattt 114960 gtaccttttg ttctttctct atggaaagat gtagttaatg gagatcctgg ataaagaagt 115020 ctagtagaac caacagtttg actattagta taagaatgta aatcgccagc aattacagtg 115080 tcataacagt tatacctcgt tagatcaatc tctggtttta catgtggagg gatttcacca 115140 cgaacatgcg tgaaacatag ttttgattca gatggtttcc actttggttt gtggatttca 115200 tcataaggga taatatcaaa ttcaggagaa cgatatggtt tagtaatgac ttcccaattt 115260 ccgtttgtta ctttattaat aactcctgca taatgataca gacatgaaat agttttagtt 115320 aacatttcat ggttcccagt gaagatcttg cctgaatggt caagtcttga catgaactgt 115380 tcaagaagtt ctatttcttc cgacgatggg tcggcaacat caagaatatc accaccagca 115440 atatgcagat cacagttatg attatggaat atgtcattta accgttctcc tagcatcagg 115500 aagcgacgct tctgccattc ctttggaact ttatcttgtc ctaatttgat atgatgatca 115560 gcactaaata gtattctcat aagttaaaaa gaaagggagc cgtagctccc cttggtttat 115620 tagtcgtcca gatcgctagc agcttcagcg tcgatgcctt tctgagaacc agcatcacca 115680 ccagacttag cgtcaccgcc gtcttgcttc tcacgacctt ccatgaaggc gagaatagct 115740 tctttctgct cttcgtagga cggaaccggg taggtttctt ccagagaagg aactttttca 115800 aacttgatga actcaccagt atcatcacac atagcttcac cgagaatatc ggcatctgcg 115860 gcgtgcagtt tagcttctgc gctagtagga tctttcagag ccatctggaa ctgcattgct 115920 gcgatctgct gtacatcgta ctcagtatcg aagcctttac ctttcttctc gatagaaata 115980 tcgatatcga acgggtcgga cagtttcagc tgcttcatga tagactggat gcctttcagg 116040 atagtagctt taacttccat aactttcagt ttgttatcag aacggtcgat aacgaaagcg 116100 atgtagtttt tcttcggttt cagcggaaca cgattaccat ctttgtccag ctctttctcg 116160 aagaagccca tttcgtgaat cgggtcagct ttaccgcgaa cgaagctctc tttagtacgg 116220 ttgaaacgca ggcactcaaa cggagctgca ttaccatctt tgttagtcag ccagtagaca 116280 taacgtggaa gaacaccaga aacgatacga acacgagtta caccgttgtt gaatttcagg 116340 a 116341

Claims (9)

  1. 살모넬라(Salmonella) 속 균주에 특이적인 사멸능을 갖는, 기탁번호 KCTC 13880BP인 신규 박테리오파지 KFS-SM.
  2. 제1항에 있어서, 상기 박테리오파지는 살모넬라 듀블린(S. Dublin), 엔테리티디스(S. enteritidis), 살모넬라 몬테비데오(S. Montevideo), 살모넬라 뉴포트(S. Newport), 살모넬라 파나마(S. Panama), 살모넬라 살라메(S. Salamae), 살모넬라 센프텐버그(S. Senftenberg), 살모넬라 타이피(S. Typhi) 및 살모넬라 타이피뮤리움(S. Typhimurium)으로 이루어진 군에서 선택된 1종 이상에 특이적인 사멸능을 갖는 것을 특징으로하는, 신규 박테리오파지 KFS-SM.
  3. 제1항에 있어서, 상기 박테리오파지는 pH 3.0 내지 pH 11.0의 범위에서 용균 활성을 갖는 것을 특징으로 하는, 신규 박테리오파지 KFS-SM.
  4. 제1항에 있어서, 상기 박테리오파지는 -10˚C 내지 60˚C 범위에서 용균활성을 갖는 것을 특징으로 하는, 신규 박테리오파지 KFS-SM.
  5. 제1항의 박테리오파지 KFS-SM을 포함하는, 항생용 조성물.
  6. 제1항의 박테리오파지 KFS-SM을 포함하는, 사료 첨가용 조성물.
  7. 제6항의 사료 첨가용 조성물을 포함하는, 사료.
  8. 제1항의 박테리오파지 KFS-SM을 포함하는, 소독제.
  9. 삭제
KR1020200004179A 2020-01-13 2020-01-13 병원성 살모넬라균 특이 신규 박테리오파지 kfs-sm 및 이를 포함하는 항균 조성물 KR102237011B1 (ko)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102526744B1 (ko) * 2022-01-28 2023-04-28 국민대학교산학협력단 박테리오파지를 포함하는 코팅 조성물 및 이를 이용하여 제조된 항균 필름

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US20120088676A1 (en) * 2007-12-28 2012-04-12 Institut Pasteur Molecular typing and subtyping of salmonella by identification of the variable nucleotide sequences of the crispr loci

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US20120088676A1 (en) * 2007-12-28 2012-04-12 Institut Pasteur Molecular typing and subtyping of salmonella by identification of the variable nucleotide sequences of the crispr loci

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* Cited by examiner, † Cited by third party
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KR102526744B1 (ko) * 2022-01-28 2023-04-28 국민대학교산학협력단 박테리오파지를 포함하는 코팅 조성물 및 이를 이용하여 제조된 항균 필름
WO2023146038A1 (ko) * 2022-01-28 2023-08-03 국민대학교산학협력단 박테리오파지를 포함하는 코팅 조성물 및 이를 이용하여 제조된 항균 필름

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