KR102237011B1 - 병원성 살모넬라균 특이 신규 박테리오파지 kfs-sm 및 이를 포함하는 항균 조성물 - Google Patents
병원성 살모넬라균 특이 신규 박테리오파지 kfs-sm 및 이를 포함하는 항균 조성물 Download PDFInfo
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Abstract
본 발명은 살모넬라균에 특이적인 사멸능을 갖는 신규 박테리오파지 KFS-SM, 상기 박테리오파지를 포함하는 살모넬라균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물에 관한 것이다. 본 발명의 박테리오파지 KFS-SM은 살모넬라균에 특이적 사멸능을 가지는 반면, 익균은 사멸시키지 않고 내산성 및 내열성이 우수하므로, 살모넬라에 의한 감염성 질병의 예방 또는 치료에 활용할 수 있을 뿐만 아니라, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제등 에도 광범위하게 이용될 수 있다.
Description
본 발명은 살모넬라균에 특이적인 사멸능을 갖는 신규 박테리오파지 KFS-SM, 상기 박테리오파지를 포함하는 사료 첨가용 조성물, 사료, 소독제 또는 세척제, 항생용 조성물 및 상기 박테리오파지를 개체에 투여하는 단계를 포함하는 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다.
살모넬라(Salmonella)는 장내세균과에 속하는 그람음성의 통성혐기성 세균이며 아포를 형성하지 않는 간균으로 사람·동물 및 조류의 장내뿐만 아니라 자연계에 널리 분포하는 병원성 미생물이다. 살모넬라는 인수공통 감염원을 포함하기 때문에 사람뿐만 아니라 돼지, 소, 닭 등을 비롯한 다양한 가축에 감염되어 질병을 발병시킬 수 있다. 사람에게 감염 시 식중독을 발병시켜 설사, 구토, 복통, 발열 심하게는 관절염 등의 만성질환을 유발하고 돼지, 소, 닭 등 가축에는 살모넬라증(Salmonellosis)을 유발하여 급성 및 만성 감염증을 발병시키는데 주로 오염된 사료, 물 등과 같은 요인에 의해 감염된다.
살모넬라균(Salmonella spp.)은 크게 2가지의 종(species)인 살모넬라 엔테리카(S. enterica) 또는 살모넬라 봉고리(S. bongori)로 분류되며, 그 중 대부분 속은 살모넬라 엔테리카로 생화학적 특성에 따라 6종류의 아종(subspecies)으로 분류된다. 살모넬라균은 생화학 시험을 통해 살모넬라 종을 동정한 후, Kauffmann-White의 항원표에 따라 혈청형(serovar)을 확인하여 최종적으로 균주명을 표기하며, 현재까지 약 2,659 여종의 혈청형이 보고되었다.
혈청형에 따른 살모넬라 감염 양상을 보면 과거에는 살모넬라 엔테리티디스(S. Enteritidis), 살모넬라 티피뮤리움(S. Typhimurium), 살모넬라 타이피(S. Typhi) 등 주요 혈청형에 편중되어 발병되었으나, 최근에는 새로운 혈청형을 가진 살모넬라균의 감염 빈도가 증가하고 있어 다양화 추세를 보이고 있다.
이 중 특히, 살모넬라 티피뮤리움, 살모넬라 엔테리티디스, 살모넬라 몬테비디오, 살모넬라 바레일리(S. Bareilly), 살모넬라 I 4,[5],12:i:-는 최근 질병관리본부에서 보고된 국내 주요 5가지 살모넬라 혈청형이다. 또한, 미국 질병통제예방센터(CDC)에 따르면 최근 살모넬라 파나마(S. Panama), 살모넬라 뉴포트(S. Newport), 살모넬라 센프텐버그(S. Seftenberg) 등에 의한 식중독 발병 보고도 증가하고 있는 실정이다. 실제로 미국에서 2017년 파파야, 2014년 치아씨드, 2012년 칸탈로프, 2005년 토마토로부터 살모넬라 뉴포트에 의한 감염이 확산되었고, 2016년 피스타치오로부터 살모넬라 센프텐버그, 2011년 cantaloupe로부터 살모넬라 파나마의 감염이 확산된 것으로 알려졌다.
현재 살모넬라 감염증을 예방 및 치료하기 위해 항생제를 주로 사용하고 있으나 살모넬라는 동물에 감염 시 세포 내에 침투하여 증식하고 감염하는 경우가 많아 항생제나 약제, 기타 생균제가 침투하여 작용하기는 어려우며, 최근 많은 분야에서 항생제 내성에 대한 문제가 발생하고 있어 이에 대한 원인을 항생제 오남용으로 규명하고 항생제를 충분히 사용하지 못하게 되었다. 이에 따라 산업동물의 배합 사료 내에 성장촉진제로 사용하는 항생제를 금지하기 시작하였고, 유럽연합에서는 2006년부터, 우리나라에서는 2011년 하반기부터 항생제 사용을 규제하기 시작하였다. 또한, 안전한 먹거리와 식품의 안전에 대한 소비자의 요구가 증대되고 있어 점차 항생제 사용이 줄어들고 있다. 세균을 제어하는 항생제의 사용이 줄어듦에 따라 세균성 질병의 발생이 증가하고 있어 항생제를 사용하지 않고 세균성 질병을 제어하는 방법에 대한 요구가 늘어나고 있다. 이러한 연구 중 하나가 자연계에 존재하는 세균을 특이적이고 효과적으로 사멸하는 박테리오파지를 이용하는 방법이다.
박테리오파지(bacteriophage)는 특정 세균을 감염시켜 감염된 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스를 의미한다. 박테리오파지는 항생제에 비해 숙주 특이성이 높아 특정 박테리아만을 사멸시킬 수 있으며 다른 박테리아에는 영향을 주지 않는다. 또한 박테리오파지는 박테리아에 감염(infection)한 후 박테리아 세포 내부에서 유전물질의 복제 및 조립 후, 자손 박테리오파지들이 박테리아 밖으로 나올 때 숙주인 박테리아의 세포벽을 파괴하는 방식으로 기존 합성 항생제의 기작과는 다르므로 기존 합성 항생제에 대한 감수성에 관계없이 항균 활성을 나타낼 수 있다. 특히 박테리오파지를 이용한 발명은 항생제 대비 개발시간이 짧고 개발비용이 적으며, 높은 특이성, 지속성 및 부작용 등의 측면에서 우위가 인정된다.
한편, 이러한 박테리오파지는 높은 특이성을 가지고 있고 균주에 따라 지속성 및 항균효과가 다르게 작용할 수 있기 때문에 여러 종류의 박테리오파지를 혼합하여 cocktail 처리하는 경우도 있어 다양한 종류의 박테리오파지가 필요하다. 이에, 본 발명자들은 넓은 범위의 특이성을 가지는 박테리오파지를 이용하여 단독처리만으로도 살모넬라균에 의한 감염증의 예방과 치료에 효과적인 박테리오파지의 분리 및 이의 응용에 관한 기술 개발에 주목하였다.
그 결과, 본 발명자들은 다양한 시료로부터 다양한 혈청형의 살모넬라 균주(strain)만을 특이적으로 사멸시킬 수 있는 파지를 분리 및 정제하고, 이를 KFS-SM으로 명명하였다. 또한, KFS-SM에 대한 특성 검토 실험을 통하여 높은 살모넬라(Salmonella spp.)에 대한 살균 및 정균효과와 온도, pH 안정성을 가지며 분류학상 시포비리대(Siphoviridae)에 속하는 것을 확인하였고, 유전자 분석을 통해 신규한 파지임을 확인함으로써 본 발명을 완성하였다.
본 발명의 목적은 살모넬라균에 특이적인 용균 활성을 갖는 신규 박테리오파지 KFS-SM를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 박테리오파지 KFS-SM을 포함하는, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 박테리오파지 KFS-SM을 인간을 제외한 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법을 제공하는 것이다.
따라서, 상기 목적을 달성하기 위하여, 본 발명은 살모넬라균에 특이적인 사멸능을 갖는, 신규 박테리오파지 KFS-SM을 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 항생용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 사료 첨가용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 소독제를 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 세척제를 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 인간을 제외한 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 KFS-SM은 살모넬라균을 특이적으로 사멸시키는 효과를 갖는다.
상기 박테리오파지 KFS-SM은 기존의 항생제 등의 화학물질에 비해 살모넬라균에 대한 특이성이 매우 높고, 살모넬라균에 감염하여 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있다.
또한, 상기 박테리오파지 KFS-SM은 내산성, 내열성이 우수하여 다양한 온도, pH 범위에서 살모넬라균에 의한 감염성 병의 예방 또는 치료용 물질로 활용될 수 있을 뿐만 아니라, 상기 박테리오파지 KFS-SM을 유효 성분으로 포함하는 항생제, 사료 첨가제, 사료, 소독제 또는 세척제 등으로 활용될 수 있는 효과를 갖는다.
또한, 상기 박테리오파지 KFS-SM 또는 이를 유효 성분으로 포함하는 항생제를 제공하여, 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주 범위의 문제점을 해결 할 수 있는 장점이 있다.
따라서, 본 발명의 박테리오파지 KFS-SM은 살모넬라균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.
도 1은 박테리오파지 KFS-SM의 살모넬라균에 대한 용균반 형성 활성을 검토한 것이다.
도 2는 전자 현미경을 통해 박테리오파지 KFS-SM의 형태학적 특성을 검토한 것이다.
도 3은 박테리오파지 KFS-SM의 열 안정성을 검토한 결과를 나타낸 것이다.
도 4는 박테리오파지 KFS-SM의 pH 안정성을 검토한 결과를 나타낸 것이다.
도 5은 박테리오파지 KFS-SM의 살모넬라균에 대한 살균 및 정균효과를 검토한 챌린지 어세이 결과를 나타낸 것이다.
도 6은 박테리오파지 KFS-SM의 흡착능 결과를 나타낸 것이다.
도 7은 박테리오파지 KFS-SM의 1단 증식 곡선(One-step growth curve) 분석 결과를 나타낸 것이다.
도 2는 전자 현미경을 통해 박테리오파지 KFS-SM의 형태학적 특성을 검토한 것이다.
도 3은 박테리오파지 KFS-SM의 열 안정성을 검토한 결과를 나타낸 것이다.
도 4는 박테리오파지 KFS-SM의 pH 안정성을 검토한 결과를 나타낸 것이다.
도 5은 박테리오파지 KFS-SM의 살모넬라균에 대한 살균 및 정균효과를 검토한 챌린지 어세이 결과를 나타낸 것이다.
도 6은 박테리오파지 KFS-SM의 흡착능 결과를 나타낸 것이다.
도 7은 박테리오파지 KFS-SM의 1단 증식 곡선(One-step growth curve) 분석 결과를 나타낸 것이다.
이하, 실시예를 통하여 본 발명을 보다 상세히 설명하고자 한다. 그러나, 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.
본 발명의 박테리오파지 KFS-SM은 축산물도매시장의 돼지 허파로부터 시료를 채취하여 분리한 박테리오파지로서, 박테리오파지 KFS-SM으로 명명하고 2019년 07월 11일에 생물자원센터에 기탁번호 KCTC 13880BP로 기탁하였다.
본 발명은 살모넬라균(Salmonella)에 특이적인 사멸능을 갖는, 기탁번호 KCTC 13880BP 박테리오파지 KFS-SM을 제공한다.
본 발명에서, 용어 "박테리오파지(bacteriophage)"는 특정 세균에 감염하여 당해 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스로, 단일 혹은 이중 사슬의 DNA 또는 RNA를 유전 물질로 포함하는 바이러스를 의미한다.
본 발명의 박테리오파지 KFS-SM은 살모넬라균에 대해 용균활성이 탁월하며, 상기 박테리오파지 KFS-SM은 각이 진 머리와 꼬리를 갖는 형태인 시포비리대(Siphoviridae)에 속함을 확인하였다. 또한, 상기 박테리오파지 KFS-SM는 열, pH에 대한 안정성이 우수한 특성을 가지고 있다.
상기와 같은 살모넬라균 특이적 용균 활성, 내산성, 내염기성 및 내열성은 본 발명의 박테리오파지 KFS-SM은 살모넬라균에 의한 감염성 질병의 예방 및 치료용 조성물 및 상기 박테리오파지 KFS-SM을 유효성분으로 포함하는 다양한 제품에 적용함에 있어, 다양한 온도 및 pH 범위의 적용을 가능하게 한다.
본 발명의 박테리오파지 KFS-SM은 pH 3.0 내지 pH 11.0의 범위 내에서 용균 활성이 유지되나, 이에 제한되는 것은 아니다.
본 발명의 박테리오파지 KFS-SM은 -10˚C 내지 - 60˚C의 범위 내에서 용균 활성이 유지되나, 이에 제한되는 것은 아니다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물 제공한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 항생용 조성물을 제공한다.
본 발명에서, 용어 "항생용 조성물"은 약제 형태로 동물에게 제공되어 균을 사멸시킬 수 있는 제제를 의미하며, 방부제, 살균제, 항생제 및 항균제를 총칭하는 것이다. 또한, 항생용 약약용, 식품용, 화장용 등 다양한 용도를 포함하나 이에 제한되지는 않는다.
본 발명의 박테리오파지 KFS-SM은 기존 항생제에 비하여 살모넬라균에 대한 감염성 질병에 대한 특이성이 높으므로, 유익균은 죽이지 않고, 특정 병원균만 사멸시킬 수 있고, 약물 내성 내지 저항성을 유도하지 않아, 기존의 항생물질에 비하여 제품수명(life cycling)이 긴 신규 항생제로서 이용될 수 있다.
본 발명의 박테리오파지는 살모넬라균(Salmonella) 에 속하는 균주에 대하여 항균 활성을 포함하는 것을 특징으로 한다. 보다 상세하게는, 살모넬라 속(Salmonella spp.) 에 속하는 균주에 대하여 항균 활성을 가지는 것이며, 이에 제한 되는 것은 아니나, 본 발명의 일 실시예에서는 살모넬라 균주 중, 살모넬라 듀블린(S. Dublin), 엔테리티디스(S. enteritidis), 살모넬라 몬테비데오(S. Montevideo), 살모넬라 뉴포트(S. Newport), 살모넬라 파나마(S. Panama), 살모넬라 살라메(S. Salamae), 살모넬라 센프텐버그(S. Senftenberg), 살모넬라 타이피(S. Typhi) 및 살모넬라 타이피뮤리움(S. Typhimurium)으로 이루어진 군에서 선택된 1이상에 대한 용균 활성이 있음을 확인하였다. 상기 살모넬라 균은 병원성을 가지는 것으로 감염 시, 식중독과 같은 질병을 유발하는 균주인 것을 특징으로 한다. 즉, 본 발명의 박테리오파지는 살모넬라 균, 특히 병원성 살모넬라 균에 대한 항균 활성을 가지는 것을 특징으로 한다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 사료 첨가용 조성물을 제공한다.
축산업이나 수산업에서 사용되는 사료 첨가용 항생제는 질병의 예방 목적으로 사용되고 있는데, 예방 목적의 항생제 투여는 내성균 발생 가능성을 높이고 가축에 잔류하는 항생제가 사람에게 전달될 수 있어서 문제가 되었으며, 항생제가 육류를 통해 인체에 흡수되면 항생제 내성을 유발해 질병의 확산을 부를 수도 있다. 또한, 사료에 섞여 먹이는 항생제의 종류가 많고 이는 다제 내성균 발생 확률이 높아지는 문제점이 있어 자연 친화적이면서도 기존 항생제의 사용에서 발생한 문제를 해결할 새로운 사료 첨가용 항생물질로서 본 발명의 상기 박테리오파지 KFS-SM을 이용할 수 있다.
또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는 사료를 제공할 수 있으며, 본 발명의 사료는 박테리오파지를 사료 첨가제 형태로 따로 제조하여 사료에 혼합시키거나, 사료 제조 시 직접 첨가하여 제조할 수 있다. 본 발명의 사료 내 박테리오파지는 액상 또는 건조상태일 수 있으며, 바람직하게는 건조된 분말 형태이다. 건조방법은 통풍건조, 자연건조, 분무건조 및 동결건조가 가능하지만, 이에 제한되는 것은 아니다. 본 발명의 박테리오파지는 분말형태로 사료 중량의 0.05 내지 10 중량 %, 바람직하게는 0.1 내지 2 중량 %의 성분비로 혼합될 수 있지만, 이에 제한되는 것은 아니다. 또한, 상기 사료는 본 발명의 박테리오파지 외에 사료의 보존성을 높일 수 있는 통상의 첨가제들을 추가로 포함할 수 있다.
본 발명의 사료 첨가용 조성물에는 비병원성의 다른 미생물이 추가로 첨가될 수 있다. 첨가될 수 있는 미생물로는 단백질 분해 효소, 지질 분해효소 및 당 전환 효소를 생산할 수 있는 바실러스 서브틸리스(Bacillus subtilis)와 같은 고초균, 소의 위와 같은 혐기적 조건에서 생리적 활성 및 유기물 분해능이 있는 락토바실러스 균주(Lactobacillus spp.), 가축의 체중을 증가시키며 우유의 산유량을 늘리고 사료의 소화 흡수율을 높이는 효과를 보여주는 아스퍼질러스 오리자에(Aspergillus oryzae)와 같은 사상균 및 사카로미세스 세레비지에 (Saccharomyces cerevisiae)와 같은 효모로 구성된 군으로부터 선택될 수 있다.
본 발명의 박테리오파지 KFS-SM을 포함하는 사료에는 식물성으로는 곡물류, 근과류, 식품가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류, 곡물부산물류 등이 있으며, 동물성으로는 단백질류, 무기물류, 유지류, 광물성류, 유지류, 단세포 단백질, 동물성 플랑크톤류, 남은 음식물 등이 있으며 이에 제한되는 것은 아니다.
본 발명의 사료 첨가용 조성물에는 품질 저하를 방지하기 위하여 첨가하는 결착제, 유화제, 보존제 등이 포함될 수 있고, 효용 증대를 위하여 사료에 첨가하는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 비단백태질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등이 포함될 수 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있다.
또한, 본 발명은 박테리오파지 KFS-SM을 포함하는, 음용수 첨가제를 제공한다.
본 발명의 음용수 첨가제는 상기 박테리오 파지 KFS-SM 또는 이를 포함하는 조성물을 음용수 첨가제 형태로 따로 제조하여 사료 또는 음용수에 혼합하는 방식으로 사용되거나, 음용수 제조 시 직접 첨가하는 방식으로 사용할 수 있다. 상기와 같이 음용수에 혼합하여 공급함으로써 지속적으로 살모넬라균의 숫자를 감소시킬 수 있다.
본 발명에서 음용수는 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용되는 음용수를 사용할 수 있다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 소독제를 제공한다.
살모넬라균에 특이적 사멸능을 갖는 본 발명의 박테리오파지 KFS-SM을 포함하는 소독제는 병원감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 양식장 등의 건물, 축체, 음수, 깔짚, 난좌, 운반차량, 식기 등의 각종 생육 용품의 소독 등에 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 포함하는, 세척제를 제공한다.
본 발명의 박테리오파지 KFS-SM은 살모넬라균에 특이적 사멸능을 가지므로, 상기 살모넬라균에 노출되었거나 노출될 가능성이 있는 동물의 피부 표면 또는 신체 각 부위 등을 세척하는 용도로도 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 KFS-SM을 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 KFS-SM은 살모넬라균에 특이적인 사멸능을 가지므로, 살모넬라균에 의한 감염성 질병의 예방 또는 치료에 이용될 수 있다.
본 발명에서, 살모넬라균에 의한 감염성 질병은 상기 질병으로 인해 나타나는 증상, 예컨대 복통, 설사, 구토, 발열, 두통과 같은 일반적인 식중독 증상을 포함한다. 또한, 임신부, 영유아, 노인과 면역능력이 저하된 환자들의 경우 패혈증, 뇌염 및 수막염, 뇌 농양 등과 같은 중추신경계 증상을 포함한다.
본 발명의 용어 "개체"는 인간을 제외한 동물을 모두 포함할 수 있으며, 바람직하게는 포유류 또는 어류를 말하나, 이에 제한되는 것은 아니다.
본 발명의 용어 "예방" 이란 조성물의 투여로 질병을 억제시키거나 발병을 지연시키는 모든 행위를 의미한다.
본 발명의 용어 "치료"란 조성물의 투여로 상기 질병의 증세가 호전되거나 상기 질병의 억제 또는 경감 및 이롭게 변경되는 모든 행위를 의미한다.
본 발명의 상기 용어 "가축류"란 인간에 의하여 순화, 개량되어 사람과 함께 공동생활을 하는 유용한 동물을 지칭하는 개념으로, 예를 들어, 돼지, 소, 닭, 말, 오리 또는 개 등을 포함하나, 이에 제한되는 것은 아니다.
본 발명의 방법에서 상기 박테리오파지 KFS-SM 또는 조성물은 약학적 제제의 형태로 동물에게 투여되거나, 가축의 사료 또는 음용수에 혼합하여 이를 섭식시키는 방법을 통해 투여될 수 있다. 본 발명의 방법에서 상기 박테리오파지 KFS-SM 또는 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있다.
본 발명의 방법에 투여되는 상기 박테리오파지 KFS-SM의 적합한 총 1일 사용량은 올바른 의학적 판단범위 내에서 처치에 의해 결정될 수 있다는 것은 당업자에게 자명한 일이다. 특정 개체에 대한 구체적인 치료적 유효량은 달성하고자 하는 반응의 종류와 정도, 동물의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여시간, 투여 경로 및 조성물의 분배율, 치료기간, 구체적 조성물과 함께 사용되거나 동시 사용되는 약물을 비롯한 다양한 인자와 의약 분야에 잘 알려진 유사 인자에 따라 다르게 적용하는 것이 바람직하다.
본 발명의 약학적 조성물은 103-1010 PFU/ml의 박테리오파지를 포함하며, 바람직하게는 106-109 PFU/ml의 박테리오파지를 포함한다. 본 발명에 사용된 용어, "PFU (plaque forming unit)"는 박테리오파지가 세균 사멸 후 나타나는 용균반을 수치화한 단위이다.
본 발명의 약학적 조성물은 약학적으로 허용 가능한 담체를 추가로 포함할 수 있다.
본 발명에서 용어, "약학적으로 허용 가능한 담체"란 생물체를 자극하지 않고 투여 화합물의 생물학적 활성 및 특성을 저해하지 않는 담체 또는 희석제를 의미한다. 액상 용액으로 제제화되는 조성물에 있어서 허용되는 약제학적 담체로는, 멸균 및 생체에 적합한 것으로서, 식염수, 멸균수, 링거액, 완충 식염수, 알부민 주사용액, 덱스트로오스 용액, 말토 덱스트린 용액, 글리세롤, 에탄올 및 이들 성분 중 1 성분 이상을 혼합하여 사용할 수 있으며, 필요에 따라 항산화제, 완충액, 정균제 등 다른 통상의 첨가제를 첨가할 수 있다. 또한 희석제, 분산제, 계면활성제, 결합제 및 윤활제를 부가적으로 첨가하여 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립 또는 정제로 제제화할 수 있다.
본 발명의 약학적 조성물은 질환 부위에의 도포 또는 분무하는 방법으로 이용할 수 있으며, 그 밖에 비강 분무, 경구 투여 또는 비경구 투여를 통해 투여할 수도 있으며, 비경구 투여의 경우 정맥 내 투여, 복강 내 투여, 근육 내 투여, 피하 투여 또는 국부 투여를 이용하여 투여할 수도 있다.
본 발명의 약학적 조성물의 적합한 도포, 분무 및 투여량은 제제화 방법, 투여 방식, 대상이 되는 동물 및 환자의 연령, 체중, 성, 질병 증상의 정도, 음식, 투여시간, 투여 경로, 배설 속도 및 반응 감음성과 같은 요인들에 의해 다양하며, 보통으로 숙련된 의사나 수의사는 목적하는 치료에 효과적인 투여량을 용이하게 결정 및 처방할 수 있다.
본 발명의 약학적 조성물을 유효성분으로 포함하는 경구 투여용 제형으로는, 예를 들어 정제, 트로키제, 로렌지, 수용성 또는 유성현탁액, 조제분말 또는 과립, 에멀젼, 하드 또는 소프트 캡슐, 시럽 또는 엘릭시르제로 제제화할 수 있다. 정제 및 캡슐 등의 제형으로 제제화하기 위해, 락토오스, 사카로오스, 솔비톨, 만니톨, 전분, 아밀로펙틴, 셀룰로오스 또는 젤라틴과 같은 결합제, 디칼슘 포스페이트와 같은 부형제, 옥수수 전분 또는 고구마 전분과 같은 붕괴제, 스테아르산 마그네슘, 스테아르산 칼슘, 스테아릴푸마르산 나트륨 또는 폴리에틸렌글리콜 왁스와 같은 윤활유를 포함할 수 있으며, 캡슐 제형의 경우 상기 언급한 물질 외에도 지방유와 같은 액체 담체를 더 포함 할 수 있다.
본 발명의 약학적 조성물을 유효 성분으로 포함하는 비경구 투여용 제형으로는, 정맥주사 또는 근육 내 주사 등의 주사용 형태, 호흡기를 통하여 흡입이 가능하도록 하는 에어로졸제 등 스프레이용으로 제제화할 수 있다. 주사용 제형으로 제제화하기 위해서는 본 발명의 조성물을 안정제 또는 완충제와 함께 물에서 혼합하여 용액 또는 현탁액으로 제조하고, 이를 앰플 또는 바이알의 단위 투여용으로 제제화할 수 있다. 에어로졸제 등의 스프레이용으로 제형화하는 경우, 수분산된 농축물 또는 습윤 분말이 분산되도록 추진제 등이 첨가제와 함께 배합될 수 있다.
본 명세서에서 달리 정의되지 않은 용어들은 본 발명이 속하는 기술 분야에서 통상적으로 사용되는 의미를 갖는 것이다.
이하, 본 발명을 실시예, 제제예에 의해 상세히 설명한다. 단, 하기 실시예, 제제예는 본 발명을 예시하는 것일 뿐 본 발명의 내용이 하기 실시예, 제제예에 한정되는 것은 아니다.
<실시예 1> 신규한 박테리오파지의 분리 및 배양
<실시예 1-1> 살모넬라균(
Salmonella
)에 감염하는 박테리오파지의 스크리닝 및 단일 박테리오파지의 분리
살모넬라균(Salmonella)에 특이적인 박테리오파지를 스크리닝 및 분리하기 위해서 용균반 분석을 수행하였다. 대구광역시 소재 축산물도매시장으로부터 수득한 돼지의 허파 25 g을 시료로 준비하고, 이를 이용하여 탑-아가(top-agar), 0.4% 한천을 이용하여 고체 배지 위에 붙이는 소프트 아가 오버레이(soft agar overlay) 방법을 수행하였다.
구체적으로, TSB 배지 225 ml에 샘플 시료(액) 25 g와 살모넬라균(Salmonella spp.) 진탕 배양액 (OD640 = 0.353) 1%를 혼합하고 이를 37˚C에서 18시간 동안 배양한 다음, 상기 배양액을 8,000 rpm에서 10분간 원심 분리하여 그 상등액을 0.25 μm 필터로 여과하였다. 그 다음, TSA 평판 배지 상부에 TA 소프트 아가 (0.4%(w/v) 아가, 영양배지, NaCl, MgSO4, CaCl2) 4 ml와 살모넬라균(Salmonella) 진탕 배양액 (OD640 = 0.353) 200 μl을 넣은 혼합액을 부어 굳힌 후, 그 위에 상기 돼지 허파 시료액 10 μl를 적가하고 37˚C에서 18시간 동안 배양하여 용균반의 형성 여부를 확인하였다. 용균반 형성을 확인한 결과를 도 1에 나타내었다.
도 1에 나타낸 바와 같이, 용균반을 형성하는 박테리오파지를 확인하였고, 상기 박테리오파지는 살모넬라균에 특이적인 용균능을 갖는 박테리오파지임을 확인하였다.
또한, 상기 형성된 용균반으로부터 단일 박테리오파지를 분리하고자 하였다. 구체적으로, 상기 용균반을 900 μl의 SM 완충용액(50 mM Tris-HCl, 100mM NaCl, 10mM MgSO4)에 넣은 후, 실온에서 2시간 동안 방치하여 박테리오파지 용액을 수득하였다.
<실시예 1-2> 박테리오파지의 대량 배양 및 정제
상기 실시예 1.1에서 확인한 살모넬라균(Salmonella)에 특이적인 박테리오파지를 대량 배양하기 위하여, 살모넬라균을 이용하여 대량으로 배양하고 이로부터 박테리오파지를 정제하였다.
구체적으로, 살모넬라균을 진탕 배양하여 TA 배양액(nutrient broth, NaCl, MgSO4, CaCl2) 3 ml에 분주한 후, 2시간 동안 37˚C에서 배양시켰다. 상기 실시예 1.1에서 수득한 박테리오파지를 분주하여 2시간 배양시킨 후, 원심분리 후 필터링하여 박테리오파지만을 분리하였다. 동일한 방법으로 TA 배양액의 양을 증가(8, 20, 200, 800 ml의 순서)시켜가며 진행하였다. 그 다음 최종 농도가 10%(w/v)가 되도록 폴리에틸렌 글리콜(polyethylene glycol, PEG)과 1M NaCl을 가한 후 4˚C에서 16시간 동안 정치한 후, 4˚C에서 8,000 rpm으로 20분간 원심 분리하여 침전물을 수득하였다.
정제된 파지는 CsCl 밀도 구배 울트라원심분리(밀도 구배: 1.20, 1.30, 1.40, 1.45, 1.50, 1.70g/ml; 22,000rpm; 2시간; 4˚C)하고, SM 완충용액(50 mM Tris-HCl, 100mM NaCl, 10mM MgSO4)에서 투석한 후 사용 전까지 4˚C의 암소에 보관하였다.
이와 같이 분리된 박테리오파지를 “박테리오파지 KFS-SM (Bacteriophage KFS-SM)"로 명명하고, 2019년 07월 11일 생물자원센터(Korean Collection for Type Cultures, 한국)에 기탁하고, 기탁번호 KCTC 13880BP를 부여받았다.
<실시예 2> 박테리오파지 KFS-SM의 형태학적 특성
박테리오파지 KFS-SM의 형태학적 특성을 알아보기 위해, 투과전자현미경 관찰을 수행하였다. 박테리오파지 KFS-SM을 구리 그리드에 로드(load)한 후에, 2% 우라닐 아세테이트로 음성 염색하고 건조하였다. 전자현미경 촬영은 경북대학교 공동실험실습관에서 수행하였고, 박테리오파지 KFS-SM의 형태는 H-7100 (Hitachi)를 이용해 100 kV의 전압으로 관찰한 후, 그 결과를 도 2에 나타내었다.
도 2에 나타낸 바와 같이, 박테리오파지 KFS-SM에서 각이 진 머리(icosahedral head)와 꼬리(tail)가 관찰되었으므로 시포비리대(Siphoviridae)에 속하는 것으로 추정하였다.
<실시예 3> 박테리오파지 KFS-SM의 열 안정성 조사
박테리오파지 KFS-SM의 열 안정성을 확인하기 위하여, 다양한 온도 범위(-70˚C, -20˚C, -10˚C, 4˚C, 10˚C, 20˚C, 30˚C, 40˚C, 50˚C, 60˚C, 70˚C)에서 KFS-SM의 활성을 측정하였다.
구체적으로, 1.03 × 108 PFU/ml 농도의 박테리오파지 KFS-SM 용액 100 μl와 TSB 배지용액 900 μl를 혼합한 후, 각각의 온도(-70˚C, -20˚C, -10˚C, 4˚C, 10˚C, 20˚C, 30˚C, 40˚C, 50˚C, 60˚C, 70˚C)에서 1시간 동안 상온에서 정치하였다. 한 시간 반응 시킨 KFS-SM을 소프트 아가 오버레이 방법을 이용하여 37˚C에서 18시간 동안 배양한 후, 잔존하는 박테리오파지 KFS-SM의 활성을 측정하였다. 결과를 도 3에 나타내었다.
도 3에 나타낸 바와 같이, 박테리오파지 KFS-SM는 -10-60˚C에서는 안정하였으며, 70˚C부터는 약 4 log로 활성이 감소됨을 알 수 있었다.
따라서, 본 발명의 박테리오파지 KFS-SM은 내열성이 비교적 우수한 박테리오파지이므로, 사료 첨가제로 이용할 경우 박테리오파지의 제형 과정에서 발생하는 열에 대해 안정적으로 제형화할 수 있음을 확인하였다.
<실시예 4> 박테리오파지 KFS-SM의 pH 안정성 조사
박테리오파지 KFS-SM의 pH 안정성을 확인하기 위하여, 다양한 pH 범위(pH 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)의 TSB 배지 900 μl와 1.03 × 108 PFU/ml 농도의 박테리오파지 KFS-SM 100 μl를 혼합한 후, 1시간 동안 상온에서 정치하였다. 한 시간 반응 시킨 KFS-SM을 소프트 아가 오버레이 방법을 이용하여 37˚C에서 18시간 동안 배양한 후, 잔존하는 박테리오파지 KFS-SM의 활성을 측정하였다. 측정 결과를 도 4에 나타내었다.
도 4에 나타낸 바와 같이, pH 3.0 내지 pH 11.0의 범위에서는 박테리오파지 KFS-SM의 활성이 안정적으로 유지됨을 확인하였다.
따라서, 본 발명의 박테리오파지 KFS-SM은 pH 3.0-11.0의 범위에서 그 활성이 유지됨을 확인하였으므로 pH 안정성이 우수한 박테리오파지임을 확인하였다.
<실시예 5> 박테리오파지 KFS-SM의 챌린지 어세이 분석
살모넬라균을 TSB 100 ml에 접종하고 190 rpm으로 37˚C에서 3시간 동안 배양시켰다. 배양액의 흡광도 값이 640 nm에서 약 0.353에 도달하면 (108 CFU/ml), KFS-SM 현탁액 1 ml를 MOI 0.1, 1, 10, 100으로 맞추고 37˚C에서 110 rpm으로 12 시간 동안 배양하여 파지의 용균 활성을 확인하였다. 살모넬라균을 첨가하지 않은 KFS-SM 배양액을 대조군으로 사용하였다. 초기 균의 농도는 희석 후 6 Log CFU/mL 값으로 하여, 2 시간 간격으로 생균수 측정을 하였으며, 결과를 도 5에 나타내었다.
그 결과, MOI와 관계없이 0.1, 1, 10, 100 모두에서 8시간동안 용균활성이 지속되었다. MOI 0.1에서는 4시간 때에 KFS-SM의 용균활성이 가장 활발함을 확인하였고, MOI 1, 10, 100에서는 2시간 때 용균 활성이 가장 활발한 것을 확인하였다.
<실시예 6> 박테리오파지 KFS-SM의 흡착능 및 1단 증식 곡선 (One-step growth curve) 분석
살모넬라균을 640 nm에서 흡광도 값이 0.353이 되도록 배양한 뒤(108 CFU/ml), 박테리오파지 KFS-SM을 MOI 0.01로 넣고 상온에서 배양한 뒤, 100 μl 시료를 0, 2.5, 5, 7.5, 10, 12.5분에 1 ml씩 채취하여 TSB 배지에 희석한 뒤 플라크 분석을 통해 상기 박테리오파지의 흡착능을 평가하여, 그 결과를 도 6에 나타내었다.
도 6에서 보는 바와 같이, 상기 박테리오파지 KFS-SM의 접종 후 12.5분 이내에 박테리오파지의 91.8% 정도가 살모넬라균에 흡착하였다.
또한, 살모넬라균을 640 nm에서 흡광도 값이 0.353이 되도록 배양한 뒤(108 CFU/ml), 4˚C에서 1분 동안 16,000 g로 원심 분리하여 세포를 침전시킨 후, 0.5 ml의 TSB 배지에 희석시키고, 박테리오파지 KFS-SM을 MOI 0.01로 넣고 37에서 5분 동안 배양하였다. 배양된 혼합 시료를 13,000g에서 1분 동안 원심 분리하여 얻어진 펠렛을 50mL의 TSB 배지에 희석시키고 37˚C에서 1시간 동안 배양하였다. 배양 도중 5분 마다 시료를 채취하여 플라크 분석을 하였다. 또한 eclipse period를 구하기 위하여 채취한 시료에 1% 클로로포름을 가한 뒤 플라크 분석을 하였다. 분석을 통해 상기 박테리오파지의 1단 증식 곡선과 eclipse period에 대한 평가 결과를 도 7에 나타내었다.
그 결과, 도 7에서 보는 바와 같이, 1단 증식 곡선 결과 burst size는 2.5 × 101 PFU/감염 세포, latent time 은 25분, eclipse period는 5분으로 나타내었다.
상기 결과를 통해 본 발명에 따른 상기 박테리오파지 KFS-SM은 살모넬라균에 빠른 시간 내에 흡착할 수 있고, 높은 burst size를 나타내 항생제 내성 균주의 용균 효과를 발휘하는 것을 확인하였다.
<실시예 7> 박테리오파지 KFS-SM의 전체 유전자 분석
상기 실시예 1에서 정제한 박테리오파지 KFS-SM의 전체 유전자를 분석하기 위하여, 박테리오파지의 DNA를 Phage DNA Isolation Kit (NORGEN Ltd., Canada)를 사용하여 추출하였다. KFS-SM의 전체 유전자 분석은 추출된 gDNA를 MGISEQ platform을 이용하여 분석하였으며, MGI사의 Whole Genome Sequencing 라이브러리 제작 Guide에 따라 수행하였다. Open reading frame (ORFs)는 COG, SwissProt, Pfam software를 사용하여 분석하였다.
신규 분리한 박테리오파지 KFS-SM의 전체 유전자 서열은 서열번호 1로 제시되어 있다.
유전자 분석 결과, KFS-SM은 이중가닥 DNA를 가지고 있으며 전체 유전체 크기는 116,343 bp의 크기(서열번호 1)로 이 중 GC 함량은 40%를 차지하는 것으로 확인되었다. ORFs 분석 결과, KFS-SM은 특징적인 기능을 가지는 212개의 ORF (open reading frame)를 가지는 것으로 확인되었다.
<실시예 8> 박테리오파지 KFS-SM의 병원성 균주에 대한 특이성 조사
박테리오파지 KFS-SM의 살모넬라균 이외의 다른 병원성 식중독 균주에 대한 용균 활성을 확인하기 위해 특이성 검토를 수행하였다.
구체적으로, 살모넬라균과 기타 병원성 균주 38종을 각각 배양하여 그 배양액을 수득한 후, 각각의 배양액과 박테리오파지 KFS-SM을 이용한 소프트 아가 오버레이 방법을 통해 용균반의 형성 여부를 확인하였다. 결과를 표 1에 나타내었다.
| 균주 명칭 | 용균반 형성 여부 |
| Salmonella Dublin | O |
| S. Enteritidis ATCC 13076 | O |
| S. Montevideo | O |
| S. Newport | O |
| S. Panama | O |
| S. Salamae | O |
| S. Senftenberg | O |
| S. Typhi | O |
| S. Typhimurium ATCC 14028 | O |
| S. Typhimurium ATCC 15812 | O |
| Aeromonas hydrophila ATCC 7966 | X |
| A. hydrophila JUNAH | X |
| A. hydrophila SNUFPC A10 | X |
| A. hydrophila SNUFPC A11 | X |
| A. hydrophila SNUFPC A3 | X |
| A. hydrophila SNUFPC A5 | X |
| A. hydrophila SNUFPC A6 | X |
| A. hydrophila SNUFPC A8 | X |
| A. hydrophila SNUFPC A9 | X |
| Bacillus cereus ATCC 13061 | X |
| B. cereus ATCC 14579 | X |
| B. cereus ATCC 1611 | X |
| B. cereus ATCC 21768 | X |
| Escherichia coli ATCC 15144 | X |
| E. coli ATCC 700599 | X |
| E. coli ATCC BAA-2192 | X |
| E. coli ATCC BAA-2196 | X |
| E. coli O157:H7 ATCC 10536 | X |
| E. coli O157:H7 ATCC 43895 | X |
| Listeria monocytogenes ATCC 7644 | X |
| L. monocytogenes ATCC 19116 | X |
| L. innocua ATCC 33090 | X |
| Shigella boydii NCCP 11190 | X |
| S. flexoneri 2457T | X |
| S. sonnei ATCC 9290 | X |
| Vibrio parahaemolyticus ATCC 17802 | X |
| Y. enterocolitica ATCC 55075 | X |
상기 표 1에 나타낸 바와 같이, 박테리오파지 KFS-SM은 총 9종의 살모넬라균 혈청형(S. Dublin, Enteritidis, S. Montevideo, S. Newport, S. Panama, S. Salamae, S. Senftenberg, S. Typhi, S. Typhimunum)를 제외한 기타 28종의 균주에 대해서는 용균 활성을 보이지 않는 것으로 보아 살모넬라균의 다양한 혈청형에 특이적으로 용균활성을 나타내는 것임을 알 수 있다.
상기 실험으로부터 본 발명의 박테리오파지 KFS-SM을 이용한 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 살모넬라균에 대해 특이성이 매우 높았고, 용균 활성이 우수하며 물리화학적 환경에 강하다는 장점을 가지고 있음을 확인하였다. 또한, 본 발명의 박테리오파지 KFS-SM은 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서 본 발명의 박테리오파지 KFS-SM은 양식 산업 및 축산업에서 살모넬라 감염증을 효과적으로 예방 및 치료할 수 있다.
이상에서 살펴본 바와 같이, 본 발명은 상기에 언급된 바람직한 실시예로서 설명되었으나, 본 발명 분야의 통상의 기술자들은 동일한 기술적 범위 내에서 다른 구성요소를 추가, 변경, 삭제 등을 통하여, 퇴보적인 다른 발명이나 본 발명 사상의 범위 내에 포함되는 다른 실시예를 용이하게 제안할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 본 발명의 범위를 한정하는 것이 아니다.
이하 본 발명의 박테리오파지 KFS-SM을 포함하는 감염성 질병의 예방 또는 치료용 약학적 조성물의 제제예를 설명하나, 이들 제제예는 본 발명으로 제조될 수 있는 제제예들을 예시적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 제제예에 한정되는 것은 아니다.
<제제예 1> 의약품의 제조
<제제예 1-1> 주사제의 제조
박테리오파지 KFS-SM 1 × 106 PFU/ml
주사용 멸균 증류수 적량
pH 조절제 적량
통상의 주사제의 제조방법에 따라 1 앰플당(2 mL) 상기의 성분 함량으로 제조하였다.
<제제예 1-2> 액제의 제조
박테리오파지 KFS-SM 1 × 106 PFU/ml
설탕 20 g
이성화당 20 g
레몬향 적량
정제수를 가하여 전체 1,000 ml로 맞추어 주고, 통상의 액제의 제조방법에 따라 상기의 성분을 혼합한 다음, 갈색병에 충전하고 멸균시켜 액제를 제조하였다.
<제제예 1-3> 정제의 제조
박테리오파지 KFS-SM 1 × 106 PFU/ml
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mg
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조하였다.
SEQUENCE LISTING
<110> Kyungpook National University Industry-Academic Cooperation
Foundation
<120> Novel Salmonella-specific bacteriophage and antibacterial
composition comprising the same
<130> PN1906-298
<160> 1
<170> PatentIn version 3.2
<210> 1
<211> 116341
<212> DNA
<213> KFS-SM whole genomic DNA
<400> 1
agtcgatttt atcgttagaa ccgccagtag tttcgcccca agacttagcc atattttatt 60
tcctctttaa agattatctt cgatttattg attgcaatta gtgggttagt gtcgattaca 120
gaacgtggta tccatatggg aacatattgc atgtccaaac tcggatcgtt tgtaaactta 180
tattcggcat aatttcgtag acttagaatt cctagatatt ctgccagttg tctattagac 240
aatttgtaag ggttatcaac aatagttgat tcattcaaga tgaatgaaga accgaccagt 300
aattggtgag catcaggctc tgcaagcatt ctattaaata gcttgacaat caggtcggag 360
tttcctctag ctaagaggta aattctctcg taatcaaaga atttcatttt tgtctctcat 420
caatttatgt atatattata cattaaatcg ggaggatttg acaagtacaa tttttatttt 480
tctgcttcgg actttcttat caatccgaaa ctttctctct caaatttatg tgtatattat 540
acaataatta tgagcagttg ccaaatgcaa ttttagctaa ttgtactgct ttctccggag 600
tcatagtaat agttttccat gcattattgc gatacacggc catacgtcca gaagcctgtc 660
ttaaaccagt acctcctttc ataattaaat ctacaacaat cggatctagc tttccgtcaa 720
caatacgttg aacgcggcct gctaactgtt ctatgagaga ttcattatta atgagacttc 780
ccattattaa acaagacagc tcgttaagag aaataccttc tgagaagata ctctgagctg 840
ctgcaagcac acagggtccg ccctccgcta catctttctg gattctcaat cggtcatcca 900
aaggagttgc acctataatc tcataggttg taacaccacg ttgtgcgaga gcttctaata 960
ttgtttggat tagttccgtc ctatcactta caaagagaac cttatgccca atagacacat 1020
ataaatgtgc taggtttata atagtttccc gatattcagg atgattatat acatcattag 1080
cacgtagtgc ccacggtacg ttctggtttc ctgataactc gacaggtacg gagtacctat 1140
ggattgtggg tggaacagtg ttattaacag ggggactaaa gatcttatat ccaaagaaat 1200
ctttgaacat aacttgtagc ccatctttac gttttaatgt acctgataga ccgatcttgt 1260
agcgagcaca tgagatctct aggaaattag tgaaagttgt agctacacag tggtgaactt 1320
catcaactat aacagtaccg aataccttag aaagattatt cgcatgtttg ttcactgttt 1380
gtatattact gaccacgatt ggtggatcaa tgttatactt tccagaacct ataatacccg 1440
gctcgaatcc aaaccattta cgaacttctg cagcccacat ttcacgaatg gatgtgttag 1500
tgcagattac cagagttttc tggccaaact tatatgcaag agctagagct aggatagttt 1560
taccaaatcc aggcttacca ttaataatac aggtatcatc gcactcttca tatataggaa 1620
gctgatcttc ttcacgtagc ttgaactgag gttctggtat ttctaccgga gctaatgtgc 1680
gtttatcaac taattcgtat tttactccct tagcatctag taaatctaga cgcgtaatgg 1740
gaatccactt aatctcttta gcaacaacac cactattctt atacataata gggtatttac 1800
tagtcattgt ctcgatgtga taagtggttt gcttactaca ataatcccag agttcatcat 1860
cgggcttgaa ataggcttta ttagatataa caaccttcat aattttattc taagtctagg 1920
aatttcaggc tcctcttgat ggacttgata aataacaggg ctattattta ccagaatata 1980
gcttatataa gctggaacat aggatagcac aaatggatac gggactttag ccacatagca 2040
ttgatacttt ccattataaa ttcttgcaga atgcagaact ttagacgtga taacatcata 2100
gaacgtagtt ttcttccagt taattatatt tccatcagaa tctatgaact ggctacgctt 2160
tgacccaact aattgggaca acattgatat tctaccacga atagggtaga gcttataagg 2220
taattctttc cgcttctcaa ataaggctaa cctacgttga gagaatgtgc caggcaactc 2280
cctgttatct agcacatatt tattatatct tgttgtaatt acggaataat caccttcctg 2340
ctcaattgat acaaatgccc gtaaagcata tacgggcaat ttgaaatcaa gcacctaaaa 2400
tccttttcac attatccaga tctttacata cagcaataaa cttatcatcc ttgtacttgc 2460
tgtggtctgg atgctcttta tccattgcag ctaacttctt atactcgaaa tctgcatcga 2520
gtaatatacc cttaacataa cgagtatatt catcatcatc aatacaagcg attgatgggt 2580
gctgtttctt catcttacca caagagtagt cacgagaacc acctttctca gaatcagaat 2640
caataccgat tgggcagcca gggatactaa taccacgatc cttctggatg ttacgaatca 2700
gaatctcgtt atattgatca atcagatctt cacgtacaat agcaaccacg gagtcgtgaa 2760
ccaacatgac gatcttcatc tcttttccta gacccaggga caggatttcc tcatccgcat 2820
caatagcacc taacaacagg ctatcagaag atgcagactg aatgattgcg ttaaatccag 2880
aacggatttc ttcaccctga acaccacgat cttcggaatg gatgttgtgc agacgacgct 2940
tacgaccaaa gtgactatag atgaatccaa agttcttgat ctgatcgtgg cacttatcaa 3000
tccaacgctt aagctgaggg aactgaccga agtaagtttc gatatagtcc ttcgcatctg 3060
caacggtaca ttcaacaaac ggttcgcctg tcttagcagc ctgttccaga agagcttcgt 3120
taacagaatg agctacttta gctgggccag aaccatacag aataccgaag gtaattgcct 3180
tagcagcctg acgcagagct gggaacagct tcttaacatc acgtggctca caggtcagtt 3240
taaataccat gtgcgcgatg tttgagtgga agtcaggata tttatcaggc tcattcttca 3300
tgttgataaa tacttgttgc atatttctgt caccagacag aacagcagca tagtaaactt 3360
ccgcagttgt taagtcccat gcgattacac gatatctagg aggagctact acacaacctt 3420
tgataataga ttcatcacgt ggtaactgtt gcaggttcag cttaccagaa gaacttaaac 3480
gaccagaagt agtcatgtgt tcatggaagc cagtacgaat gcaaccatct gcatcgatgc 3540
ttagaagaat cttctcaata taagtagaga tcagcttagt cagcttacga atctctaaca 3600
gagttttagc aatcgggtgc tgagtagcca gttcgttcag agcttctgcg tttgtagaat 3660
ctgcacctgt atccgtcagt ttaccagttg gagtcagacc aacataatca aacagaagaa 3720
cacggagttg cttaacagag ttcgggttaa acgcttcgtt ctgatctttc tctagctgaa 3780
cgacttctgg ataagtatac agcttctcac gagccttatt cagattgtga gttaactgat 3840
actgagcttc tttcaagcga tcaacagaaa tcggtacacc acgatcttca acacgttgca 3900
ggaatacaca accaggcatc agaacatcat aatacagact gcaaagcttt tcgttcttct 3960
caatttttgg taagaagaag ttatgcagac gcaaagtagc atcggtatct tttgcagcat 4020
acggccacat aatatcaaat ggaatcagat catagctgaa atcctctttc ttgattttat 4080
gtgctttgca ataatcatcc ttaaacttat ccagttcaaa gtcgtaatcg cccatgtcag 4140
tatatttcat agctagagat ttcaagccgt gagtaccacg acgttcatca agaacatagt 4200
gctgtaacat ggtatcatga agtctacgct ctttatgtgc tttctcaaag gaaagtccta 4260
aatgatagga ataaaagtgc atatcgaact tcaagttatg aaaaacgatc tgatgattct 4320
tgctatccag aattttctgg agataatgta cagctacttc ggtgatgctg tcagagtcaa 4380
tataaacacc ctggtactct tggtgagaca tagaaacacc aagcagataa ccatcacgag 4440
cgtacaaagc tgaggtttcg gagtcgaatg cgataggacc gataaccatg ttatacacca 4500
tcttgatgta ttcttcagct tcatctggat cagtaatagg acgataatca ccagcttttg 4560
caatcttctc acgaccatta ataatatcgt gaatactttc tacagttgca tcgaaaactg 4620
gtttcatttc aggtttaaag tgtaactgag ctggactaat acttgcaatc caattggcat 4680
agccgccatg ctctacacgt ttaccagtat aatcaccgat acctttctta cctgcgaaat 4740
acaggaaagg ttcagcacca actagtacaa caaaatcgta atcgttcgga tcgaacgggt 4800
tatctggagt tccgattgta atatgctttt tcagcaaacg gccagataac ttctcgttac 4860
acatatggaa tacatcaact tcctcaccat aaagctggaa gtgtttatca taacgagtgt 4920
tatttagagc tttatcaact actgcaattt tcaaatttag tctccttttg gtaagtaagt 4980
ataacttcag tgtttcttct ctaacttacc aatatattat accaaatctt tgagcgattc 5040
ggcaactaaa atttcaatac gttgagccaa catatcgatc tcttctttat ttaaatcacc 5100
tgggtctttg ccttctggaa gaaggaagtt accaactaca ggtgttaaac gcgtttttgt 5160
acgaattagc ttcgctagtg cttgtgcggc tttattacca gaggcatcat tatctaatag 5220
gataactact actcttactc cagcaataat gtaaggactg aacttatcgg caatgttatc 5280
agatgtaaac tgatgtgtac caaagcagca agaagcataa tccacaccgt tatcttctag 5340
atttaacata tcaaagatac cttcaactag aataagaact ggtgtattat atcgtacagg 5400
gaaaatcggt ggtgaaactt gttttggttt tactaagtat ttaggagggg cagaactgtt 5460
tatagaacgt cccaggaata ggatattgcg tccaacagca tctgttattg ggaatacaat 5520
tctgccttcc cagtctgctt gatgttggaa agcaaaatat ttcttcaagg tcttagaact 5580
gatacctcgg aaatcacctt cgaaaaggta agcagattca ggaattgcaa gattcgtaga 5640
tccattccta atctctgaaa tctttctacg tacttgtgac aatcttggtg actgtctgta 5700
ctgagtctca ttaaaataat ggtaaatgct tggtattcct ctaccgaagc cacaactcaa 5760
gcagtgcata atacctgatt caggatcaat acgcaaactt gggtgtttat catcatgatc 5820
tggattgaga caacagatga ggatgtcccc acctgtgtct ttgtattcaa tgcctttcag 5880
atcaagtagt tctgttattc tactcatata tcgctggcct gttcacctgt tgaattttca 5940
gctttgtctt tcttagcacg tttaggttga gctggtttat ccttttcaat aggaataacg 6000
aactcagctt ccatttgcgc aatatcttcc attgctaggt tggtagtatt atccattcgc 6060
agagtttccc agttcatctt aggcataaac ttcacactat cagaggaacg agtcttaacg 6120
aagtcaaaca taatagcacc ttgaccgtta tcagcttttg cagcattaag attagcagcc 6180
atatctgcgg aatcaagaat ccctttagac atacgcgttc taccatcttg gtcaatctgg 6240
taaggggcaa caccagccac gttatgtttc tggcagatag atttgaaaga tgaactaaca 6300
accatctgtt ctttccagtc atacatgtca atggtctttg aatctggaag tcttgtttgg 6360
ttaatatagt ctagaagagc tacagtaacc ttatcaccat atttagcaac tagtttattt 6420
aattctacgt caaccgtcgt aatagacagt tcaggatcgt aaacgatgat cataggagtg 6480
tgaagctcat agccttcaag gagtttactt tccatatcat agaagtcact cattttagcc 6540
atagtgtatt gcttaacgaa gttatcgaaa agctcttcac caccattaaa cattctagcc 6600
ctagttctgg ctaatctcaa cagagcagca ccttcaagag tgttattacg cattgctaat 6660
gcagatactc cagctaacat agccagatta cgtctaaaca cttcgtgctc tttcatctca 6720
attgagaaat atggagcaat gtctccattc aaatattgct gaacctgtat gtttgaacag 6780
ataatggatt taccagtacc acgccaacca ccaagcagta acgtttctgt gcgagctaaa 6840
ccaatttgag cgtcgaactc attacaaata ccaagagcga tcaagttcag tttggtatct 6900
tcttctctct ggaaaatacg cattgtgtct gcgttgaata cctttccagt attcgttact 6960
ttctcttcta attttaagtg aagggaggca actcggttga gaatttctcc ctgatccagc 7020
attgttaaat cttgaagcac gtctgtttct agaagtttca ggaatagatc ctgtgtatat 7080
tcggcttcta ggacttcaag cgcctgttcc atgctcactt ctggaatttg agtgttagct 7140
aagacgacaa gagcttgaga aaggcgggcg ttcctattgg cctcaagcat cagtgcgtca 7200
atggacggca ttgcgttata tttcttataa taattctgga cggcttggta aattgaggag 7260
aaagcgtcat taaaatgatc tttatgcagt tttgagaatg tttccaatgc tatttgcttc 7320
tgttcagaag cgagaagcat cttcaacact acagcttgca cgttaaacaa ggtcattctc 7380
ctttgcacgc tttcgtgcct tctaaatgca aaaaggggaa ggagcatagc cccctcccct 7440
taggtttaat ttaccagatt attcagcagc tgcagctttt gcatccagtt tagcacgctt 7500
agcggcacca tcatagtcct tagcaaccag accacgacga gacagcatag atttaacacc 7560
gcgctcagac ttaccagttt tctcagcaac ttctgcaaca gtcatagaag ccagatccag 7620
accttctaac agatcttcac gagtcttagc acttgatgtt tcctgtaccg gcatagcagc 7680
gatacgacct tcacgaagca ggctcagagc tttaccacgg atctgcttaa tattacgacc 7740
gaaatgagca gcgatagctt caatagtagc accagcaaca acctgattaa cgaaatcagt 7800
ttcttcgtca ggagtgaagg aacgaacagc agcagctttt tcagttggct taacagaagc 7860
agtcatttcc agactcagga tcttaccctg tacctgctta gcaccgaact gaccaccagc 7920
tacagcagca gcgatttcag cgtaagtata ctgaccagca tgagcgttca ggaaatcaac 7980
tagttcagct tcctgctcag gagtccacgg ggatttctgt acttcgttag ctttctgtac 8040
ttcgaaacct tctttacgca gcttagagcc aacagagcga gcggtaacgt ctttaccagt 8100
ctcggtagcc agttcagcag cgatagcagc tacttgttct tgagagatta cagcaacacc 8160
cagagcagtg gcttttgctt tcagagactc ggttacacct tctacgttcc agttcagttt 8220
agacattatt atttttctcc aatagttctt taatcgacag gatttctatc ccattcgttt 8280
cggctttctt ataagatgag gaagaacgtt ttgattcatc ctcacagatt aggtatttga 8340
cgtctttggt aacggacttc ttaaccgtat atcctagact ttctaaatag tttgttgcat 8400
ccgttcgatt tgcaaaatct tgcaaagatc cggtaataca gaccgtgatt ccatttggct 8460
gggcaactaa atcatctgta atgatgatat ccgctttagc acctcgaata cccgttgaga 8520
atttccacgg taattcaata acatccttgc cttgtgggga atttagccag gttttgtaat 8580
tttctccagc cttgccgtca gccttcacat cgtggaagct agtgcaattt tgggacagtt 8640
tctttgctgc aacctctcca ataagaggaa ttcctaaaga accgagaact gaaccaaagt 8700
caatgtctcc gcgaacctta gtatttaact cgctaatcaa tttagcggca accttgctac 8760
ccacagctcc gaccaaatct tcttcggtca ggtaaaacag ttctgagatc ttagtcagct 8820
ccagcttctc aatagttttc gggccaaagc cctttaactt catttttgtg cagaagttct 8880
cgattaactt acttgattgc gctggacagt tggacttatt cctacagaat aattgcccgt 8940
tgacaagatc tagcttagaa ccacaagagg gacattgtgt tggaatttcg attttcatca 9000
aatttcttcc ttatcaattt atataaatat tatagcaagt atttaagcat ttagcaacta 9060
caattttaac taactttgct taccctcgcc ataactttct atccctcaac tgaatgataa 9120
tagtatatac ctaagcggcg aaaatgtcaa taaccacttt ataattccga catggtaaac 9180
gggtagcaaa gggttacgaa ttagtcgtat actcgctcta ctatacaagg gattacacca 9240
ccagcacgaa tcactcgaat ctggcaaccg atctctaaat cgagagcgtt aatataatca 9300
acgttattaa gagttgcttt aacgattgtg gcatcatcaa taatcaccgg ttcgaaataa 9360
ccaactggag ttactttacc agaagcccct acttgccatt caactttaac aagtgtggta 9420
atttcgcctt cttcatcctc tttaatagca aatgcaccac gaggaaattt attagtccaa 9480
ccttcacgga agaatttatt gttgtcgtta atgcgaacaa ctttaccatc tgtcggaatc 9540
catttaaaga aggaacgaac attaataact gtcaagaagt tctcattctc tagccaaagc 9600
atatctttta aataggcttc tgtaataccg actgtttccg ctgaacactg gataccatat 9660
gcaacgaaga ttaaaccacc ttcaccaata cgttgcatga aatcatcact atctttcagg 9720
ttaatagcac cagaagcata gttacgtttg ttctctactt ctttagtaat gagaacttca 9780
ccagtgattt gagttggtac tttttgggaa atagttttgg ggatgttcag cattttaaca 9840
ttatgcgtta catcattccc tagaataccg ttaccacgag ttaacgcttg aacaaattca 9900
ccattaatat ataatagaga aatcgcacaa ccatctaact tatcagtttc aacctgacct 9960
aatgggttaa atggagcttt atcaccacga tcgtaataga ctttctgtaa agaatacata 10020
cgatacaaat gagggatatc acctttcggt ccgatttctt cctctagtgg gaatctttta 10080
atcagactat catattcttc atcggaaata agactcattc cctggtagta cgcttcttga 10140
caacgtttga taaagtcttt tacattattc atcgtctgcg atttccattt tgagttcttt 10200
attatactga gcagccatac cagctaaatc agcagcttct ttcaaccacg catctttgta 10260
cgctttctgg tttggagtgc cgatactatc ctggtaattg attcttgcta tggagagcaa 10320
attaagtttt gcttgaattt ttggaagtgt tattgccatt tactgtacct catatgcttt 10380
aggctgaata gtatccccct gccatgtaaa gttcttacat aagcaaagat aggtagtcct 10440
tcccactctt tgagttattg gagagtaaat atcagcaact aatgtgtttg cttcgtaagc 10500
gagagatata agtttatatc cctgtcgaac catacgtacc ttctctccat tgagcttttc 10560
ttcccaggaa agaactaaag actgccctat gtacggtaaa ggtttgagtg tacttatttt 10620
tatctttttc atattcaccc ctctcattaa tttatataac tattatataa gaaaataggg 10680
atgaaagcaa gtacatttat aaagaaaaaa gccaagaact aggcattctt ggctttgagt 10740
tcttcagctc gtttctgtac ttcatgcaag acctctgatt cactgaggat ttgcgtaaaa 10800
gcgtagaata gctgtgatgt agtttctagg gtataaggaa aagagaagcc ggatttcgtt 10860
ggaaaccact ctccagtaat atcttggagc cagtaacgaa ttcccatgta tagattccca 10920
cgaaattcag atacggttag tcttacttgt tccccctcct tctcccaaag catgatggac 10980
tggtcgtcaa cgtgtccttc atagttttga tttacctgat cgctcattta catatcccaa 11040
atacacaaaa agccccatat aggggctaat tggtttctta tcggcctact ggagaagcac 11100
gatctaactc tgactggagg ctcgtcacac ggcgaacttt cgcaacagga atgaaacgga 11160
agctatcgtt agtacgagag aaaacgagga tttcatcttc tttcggctta cggatgcgct 11220
cacgtttaat acgctcagcc aggtatttat cctgagctgg atcaaattcc attgtaccct 11280
gaagatacgt ggttcctttc tgacgaagtt tttcgtaatg aagataaaac tcaccaaatt 11340
tttcgcattg agcaataatt tcagctttat tcatgactgt attccttgtg ttggtggatt 11400
tatctaagaa ttacttagtg atagctcgga tagcttctgc tagatgagca gctgctttac 11460
cagtcagctt gtcaatgatt gcgtcgtcca ggaagtccgg ggctaagcca gcatcagaga 11520
atgcagagcg aagatcggag tgagcctgag ctttggaagt acgagaacca ccagaagctt 11580
tttctccagt agatgcgctt gatttagaag tagagccagc agctttcttg atatacagac 11640
ctgctttggt cagcttcata cggaaaccgt tcggagttac accattttcc tgagcaattt 11700
cgctaacaat ttccatgcta acgcccggac gctcatcttc tgggaattgc tccatacgag 11760
caacgtagtc ggaagacatt ttttcgaaca gttcatcagt ccactgagtt ggagtagtca 11820
tatttttcga tttccttaat taagaattaa acagaatagt tctttcaaac tatgagaata 11880
ttatatcaag aattacagga ttaagcaact gaaatttttc agtagttact ccaccaggcc 11940
aacagcaagc atatcatcaa tgctggcaat ctcccaatcg tcggcaatag cttcgcacac 12000
tgttaacgta acaacctcac tatagggata attaccagct acccaggtat gttgagtaac 12060
tacatctgag tcagcgtcta aagttattaa cactaagcca ttattgtcat tatcccagct 12120
tctacgatat agagttttta cttccccatc tttaagcaaa tctgttaatt tactcactgc 12180
gtcttccttt ttctttatca agtttcaggg cagctttaac agcttcattg actaatgtga 12240
ttacttcttc acggctatac ttgaatccta aagccttaac atcgactccc agctcttgca 12300
ggtgttttac agaagctaac tcgtaattca tatagtgaac gttctgttgt ttaccttcac 12360
ctagtaacca aatacgataa gcccctactg gattagttaa tgctttctta acttcaccaa 12420
tacactgata accaggaacc caaacaagct caccaacttc aaattcttct gccattgcat 12480
catctggaac cattggggga ctcaacggat ccacgatatc gttaattcga agcagggcac 12540
cataacgttc cagaacagat ttaatcatcg ctacagaacg atagttacga tctgcgattt 12600
cttcaaaaga atcaccagaa agatactgtt caattacatt tgcaagctca acaccttcaa 12660
tcagtgttcc acgcttcttc tttttcattt ccgcaacttg aacctgacga tctctccatt 12720
cttcgatcat tcgttccata gttgggttag aagaaacacc gagcatttca catgctgctt 12780
tcttagtacc accattctcc agatgttcaa taaccttttt aaaggtttca tcagggattt 12840
catggataag tttctttcta cgagaaccag ccatatttaa actcctctct caaatttatg 12900
aatctattat acagaaaatt tttcctgaaa gcaaataaat ttttaagctg gatatagctc 12960
ggctttgaaa gcagctctaa cttctatggc ctcttctaac gaattgcatt gttggtactt 13020
acgagtattt cctttttgca taagtacaca gtatttttca ccgcgcatat agatattaga 13080
ttctcctaac ttattgtcct ttctcacatc tatattatgc atttgatcag atctattgga 13140
atcttctaga ttctctatga aattacaatc cttaattctg ttattatggt ctatctcttc 13200
aggaatatat cctttatgat acagaaagac taactgatgt tctccccatt tcttgccttt 13260
gaaaccaatc ctgatatacc ctttgtgctt cccacccgca aactgcccta ttaacctctt 13320
ttggttagtt ttcctccatt tgaggtggcc agtttctgaa tcatattcaa aggcttctct 13380
aacctcttct tgagttatca tcttctttaa ttaattctcc taattcctta taaacacgtt 13440
ctaactcaaa agggatacgt aaggatattc gtaccttacg ttgttttaac agccatatag 13500
atacgttcgg aataccagcc aatctgggag ggaccactcg gcatagtttc tccgcagtat 13560
cgtaaacaga agttttacgt tcttttacgg gaaacttcct tgtttcccac tttttattct 13620
tcactatcat ttctagctcc caattcagaa agagcttcct cgtgcatctg acgttcttcg 13680
tctgtcataa acttaactgg aatcggcata cattcgatct tacagtaagt tctgtaccag 13740
tccacaatct tttccgtatc cagatcttta ccgataccca tcatgccaag atacatacga 13800
gcatattttg ggttagcact ctgacctgtc ttaaggaaga aatccttctt cttattctta 13860
agagcttcga taaacggttt gattgttacg tttgaacact ttttaatatc ttcccagaac 13920
atgtcgtgca tcttatggaa gaatgcagct ctatcgtttg gtttatcctt gatatactgt 13980
tcaacgtgtt ccactgttac atctttaata gaatccagat gataatagcg aaccagaagt 14040
aacttggctt cataggcatc tgcatgacgt ggtgcacaat attcaggagt tgagtgcaaa 14100
atagctttga ttcgctcttc tgtatactct ttgtctgcac cttcgatagt tccccagctt 14160
tcgctgaaaa tatcgatagt tgcaccctga tcaagcagat aataacgcaa agtactagta 14220
tagtttctag attccagctc acgacgacgt gcgtacagtt gttgggctaa ctcaatccaa 14280
ccttcgtcaa tattatattg ctcggaaccc gcagaaaatc cactcgtaga tttatcaata 14340
aaataataaa tgttctgagc accacggtcg cgcctgattg cttgaaaacg catgtttggc 14400
gcttggtttg aggttctagt gattacgaat acgttatcaa aatagttaaa gtcaacacca 14460
ctcgttacag atggactaca taacaaacaa tcaatctgtt gatcaattag ctcattagtt 14520
gtataatcca gaatacgacg gatatctaca tcagacgtag agtttgagtg aatttcttta 14580
actaacgcac ccgtattacg acgtaatgcc attccctttt cattcagttc atctggtcca 14640
cagtcagata ctaggattga cttctcaccc atctctagag atgtttgaag tgcaacccaa 14700
atactggatt cgtcagggaa ttcataagca tgagcttttg ccagcatttt acggtgatga 14760
ttatagaacg caaccggttt atcaaaatca atcaaagaac cataggcttc aatcgtttct 14820
gcactgatat ccccatcaga taagatcacc gtttttgcag taataagaat atcacgaaga 14880
acctgaatac attcacgacg gtttttaaca accggagcga ataacagatc attcattact 14940
gcgtcacatt catcaataaa gatcgtatca atctgaccca caaagctctt gaacttatgt 15000
atagagtgaa tagttgaaga catacgatca atagcaccac gtttgaagtt cagcatatca 15060
actgacttat catactggcc agcaccaaat ttcttcgcgt tagaagatac aagtgcccta 15120
gtatttgtga ttgcaataaa gtttcctgga agaacacctg catctagcca cttagttact 15180
gctgtagttt tacccgtacc aagacttgcc ttaacaaatg ttaaatgtcc ttttggtggt 15240
acaatgttaa ttttcaggta tggaacttct ggtggagaat ctgtgtctag acgttttaat 15300
ggtacacctt ttaagtttgg tggaatctca cgtttggagt tatttacaaa ggctttgaga 15360
gcctgcttac gaccgttatt aaagtaatct tgaatattac gactattatc tttagttgca 15420
atatattcag ataaagctgg tttaatctca cgttctaacc acgtaaagtc aacaccatct 15480
tctaacgctc tgtgataaag ttttgggata atacgcaggt acaccccatc ctcagcttct 15540
tctagttcgc tgatggtttc ttccacttta tcagatgcag ccttcttgcc cttaatttgg 15600
tcaagtaagg agtagaattc ttctttaaac tcacctctag ttgtttcaaa gtcaactagg 15660
ttattgggta gattcacttt cgaaccctta acatacacaa gacgtggttt attctccgct 15720
ttaaacggat cgactatacc atcatcaaat aagggatctg cgaaatagtg aagctgaaca 15780
gaagaataat aagctaagtc agcaatatca aaaccatact tttgcttact actgtcattg 15840
atagatgtaa ataagaactt taactgtccc tgcgttacct tgacattaga ttctagcatt 15900
aagtgcattc ggatacctgg ttttaatcca gccgaagacg atgcatgggc aataaaccct 15960
gcatcaagag gaaacatatc ctcactaata ctattcaaca tcttaataat atgacgtgcc 16020
atgccgacaa gattaaactt gtcatagcca ccagtatcta caataccatc cacgtccata 16080
gcaataatat gactaggatt ggatacatta aagtttcctc tggtacgacg cacattatct 16140
tttggagcta aacatttacc acgaatagct acaatatgcg ggtctttggt aagacgacgc 16200
atgataggta acatttcagc aaggttttgc gggtcaagtt cttcaagtac atcaaatttg 16260
aagggcatgg aagctggttt accttctgga tgctctttag agaatcgctt tgcaaaggtg 16320
tagtcttcca ctttgatctc tttccagatg ccagtggctt tatctctgga aaatcctgcg 16380
tgaccttgta ggattgagaa cacaacaata ctcctatgtt ggttattcgg atatctttct 16440
ttagggctat tcaaaagaat agaacaacta ttactaccat ttacacaaac attaatcatc 16500
atcaagcagt atgaataagt tatcatacct cagtgctgcg ctctcttaac tcttatccag 16560
atcagaacct aggtcattgg ccgctaagcc gattctgatt gccctcacta tcctattggt 16620
aggtggctac tggacgtgtc accattcgct gctcttttcg ttcgcacccc catataaagc 16680
tctgcctata taacgagcta aatatgcacg gtttggattt acctttccga attcagggta 16740
cgcagatcac ttaattgata attaactaat gcagtataaa cactagtaat agttaaaaca 16800
acccttaatg gggcagccta actgttccgt attgcggatt gtaaacaaga aaaattagca 16860
aaatcaattt ttactaatgt ctctcattta tgggaatatt atactataaa ataatactcc 16920
cttgcaaatg aaattttact ggattgattc cctaccgtat aattggcctc taaacatctt 16980
ggccatagaa catgcaacct catagttgtc atatgttcct ttaactatcc gtttacggtt 17040
aatagtaaat actacttgcc agtttttact accagaaggg taaacatgca ttcctttttt 17100
atacttggta tttcttagat taccagttac tgtctgatct ctaagatttt caatacgatc 17160
atcacctttt atagtgttct catgatctac caaatcaggt aagtacccat gatgcattaa 17220
aaatattgct ctatggcgaa gcatagtcct gcttcctaga gatatacgat aatacccatt 17280
acctgtagaa acacaacatt ctttccctgc atttcttcct gtagttctaa ataacttacc 17340
gtcttcatat cggaagttat ctaaaagttc ctgctgtgtg aatgacatcc tcacctccaa 17400
tacatatatt attacataat ccatccaaat tgtcaatgac aatttatttt gacaatcaat 17460
cgactggaaa gattaggtaa taatccgagc actaatagcc cggattatag agtttagtta 17520
atgaaaatca atcatcattt aggcctggga catagattcc aatgcgattt aattgacctt 17580
tagcttcttc gagaatatct tcggtgtact cccaaatatc atcttctact tgcaaaagat 17640
aaggggattc accagtttcg gggtcacgtg taatggctaa gccacgtgga tggaatatag 17700
agaagttgag ggcaacaatc aagcccatac tggacaattc attccagtca gcttcccaac 17760
catcatcttc atcttcgtcc acaaagacac ctacactagg gtctgttaca gaagtaaacg 17820
gaaatgttac actaaccttg tcaaccccca accaacgtgc aaggagccct tcagcacgct 17880
ctaccaaact aggatctatc tgacgccaaa atacttgttc cattctgcct ccttaagcag 17940
ttaaacctgg gatcatatac ccgaaacgac gaagtttctg aatcccctca tttacttcct 18000
cgggtgaata tgaccagccc tcacgttcat ttacctggaa agcaggagct tcaccagtgc 18060
gataatttac aataagacgg tgatttgcag gcataaacag cttatggttc aaggcaaaaa 18120
ccagacccat ttcatcgagt tctttccagt ctacaatctc ttctactccc tcccctaaat 18180
ccacgctaac tccaatacac ggagcttcct ctagtgggcc tttaaaagac aggaagatat 18240
cgttattctc acaccaaatc ttaagcagat tgatggtttc attgtacata ccattaggga 18300
gagaacgcca gaagttgagg agcataggat ttacctcttt catgaaaata ttattgaaca 18360
tgcttatacc ttaagtttct gaagagcctt acacatttca agatctggac taaattccag 18420
catatagctg atagcattta aatcttgaag gtttattcca gtcctgccat ttacaataac 18480
agtaggaaat tggtacatca tagcaccagc atcatctagg acgcaccaat gcttgagctt 18540
atgtttttct atgaaacgta agacagaatt acctcgagat aaaccacctc cagtaaaatc 18600
tgttgtacca aggaaacgat cgatcaaacc gagcccggtc ataatctgaa cgttctccat 18660
ctcgtttcgt acagaaaacc atgaagatac cccaacaatc attattggag aaggtttaag 18720
gaagttctga aaagcctgga gtaatggctt gtaaacccaa tcactgccaa agaacagctt 18780
ttcattaact ggagcatgat ggtgagagat gctcgaattg agcaccccat caatatccag 18840
gaaaatcaca ggagtattac ttatttccac cagtagcatc ccacgtccag actttaactt 18900
catcagtaaa aggaactgga accttcagct cacctttacc ccacataaca gatagaaaca 18960
gagaataaat ggtaatcata ccaattaggt tgacaaacca tactagaccc ttccagatac 19020
ccttaaaaat cttcttcacc atactctccc tccttaataa ggagctcttt ccattctgct 19080
gtatcctcat agataacgca gaaatcatca cttttccatg agaagtcatc ggtagcaaat 19140
tcgaaacggt agcaccaact accgtccggc catactaaaa gatcacacat gcttttgttg 19200
gacatagggt ttaccttcta gatgagcttc cacacgagca ataaaatcag gatcactgaa 19260
ggcatccacg gcacctttac tccagtaagg aacaccacgt tcttctaact gatacatctc 19320
actacgagtc ataccctggt agccaggttc catagctact gcactatcat aatagttaac 19380
taccataagc tttgcagcag taagattcag aacaggaaat agctcccaat agaagttaat 19440
tacttggtta gagcatccaa caatgatgat catatcctga ctggtaatac catcaaaaac 19500
ctcatacatt gttttatatg cgggagcaac ctcaccaaag aagataacgt taggtttaac 19560
ccaatcaaaa tcatctggat caatagaatt gtacccaata tcaataatat cgcgtgtctg 19620
gtcgaacgca tgctctacga taatttcttt cagatatcca tgaatatgga gtatatcttc 19680
tctcggcact ccagcacgtt cgattagatc atcaacattg gtagtaaggt ttaccacttg 19740
atttggatac cgtttatacc attcaccaat acgcaaatga gctaggttag gttcaacagt 19800
ttgtaactct tcacgacgtt tgttgtaaaa catatgagtt ttatggtaaa aattaccacg 19860
aaaggcatga atattacata cctcttctag ggcataatca tcccacaacg ctttaccact 19920
agcagtatca gtacggaaag cacgaacacc actatctaca ctcaaacctg caccactaac 19980
aatgattagt ctacgcatta tacgattcct tgcttgaatc cttcgatgat attttggagc 20040
agaattgctt cttctgggcg aaccttctct tgcttaataa gcatatccaa accagcaata 20100
caggtagcta ctccagcatt atacccagca ttccactgct ccatgagcat attttctaaa 20160
ttacttttta gattctgatt caagaagtcc ataaagtttt tcctccaatt ccataatacg 20220
attttcgtaa gaagcacgca gcttctcgat ggacaaagca cgaactgtaa cttcatctga 20280
attgatcgct agctgagcta gatcttccgt ttccatacgt ccgttgtaaa ttaagcgata 20340
tttaagatgt tttacgtttt tcattcttgt tccttagttc tgtactgtaa attcattctt 20400
atcacagtga gcataataat acccatacat acgataaatt ttcacagcct tagtagaccc 20460
cttcgattct tcataatcaa tccagagcca cttgtcttta cctacataat gtacccgaaa 20520
cttcttaact ttcttatcat tccatgttgc aacaacacga cgataatcaa atccgcccaa 20580
ccaatctaaa gccttacgtt cttcactagg ctctccaaga tgggaaacat ctactttcgg 20640
aagcaattcg ctcatagctt tctcctctca aatttataaa tcaattatac ataagtttta 20700
gctcgaaagc aaataaaata aaagccaggt cgactagcaa cctggctgat tcattattta 20760
gcgcgaatgt cctgatgttt cactcgaccc ataacctcat gttgtttacc tgggttaaac 20820
ggacgagcac ctggatttcc aagatatcca caaactcgac gagttacctc aagagtctca 20880
ggatcatgat tgccacaaat tgggcatttg aaaccatcct cagaagctat agtttcaccc 20940
aagaacccac attccccaca agagtctact ggagtattaa taccgaagta atgaacttta 21000
cttgcagcat agttaataac ccattccagt gcatcaggga atcgtttcat atccggtaat 21060
tccacatagg aaatgcaccc accagaagca atgggagtaa agttagactc ataatcaaac 21120
ttcacatttg gagcaacctt acgttctaca tcaaggtgat gggaattagt gtaatatcct 21180
ttagccagaa tatcttcatg ctctgggaaa tactcacggt ctagacgaca gaagcgatca 21240
catagagatt cagatggtgt agcatataga ctataaccta attgagtctc ttccttcttc 21300
tgatcagcac gatcacgcat atattgtagc acacgttgaa cgaacttgat acaagactca 21360
gacattgtat ctacatcttt accaaacatg agctgcagca tttcgtggca accaatatat 21420
cctagagata cagaagcacg cttgtagaaa tgatcgaata catattcatc tggccccaga 21480
cgtaatccaa aggctcctga catatagagg atgggcgcag ctttagcttg aatgtatttt 21540
aagcgatcaa ctctccactc caatgctttt aacgcggtat ccacacgcag ctcaagcaga 21600
tcgaagaata gatcaatatt tccttccgct tcgatagcga ttcggggaag gtttacggaa 21660
acaacgccta agttgttacg accagaagtt tcaccatctt ccgcagcagc caggaatgaa 21720
cgacagccca tagagacttt gtagtctccc gtaacttcta caaccttatc ataactgatg 21780
tagtcaggat acatacgttc agcagtacat ttcatagcta acttcttgat atcatagtta 21840
acatcaccag gtttcatatt tacgccatca cgcaaaacaa ataccagttt agggaagata 21900
gctgtacgtt tattgatacc caaaccatcc atacgcactt ctaagattgc tttctgtaca 21960
agacgtgctt cccaggaagt tcctagacca aaaccaaagg taataaatgg actctgacca 22020
ttagagttga acatcgtatt gatttcatac tctagacctt gacaggcatc gtaaacttct 22080
ttctcagtca tttccgtagc cattacagat gctttggctt catccttagt ccaacgcttt 22140
gcaaacgcat gattcttatc gtaagactta cgaacataag gagccaatac ttcatctaat 22200
cggtctacag aagtaccacc atattgatgt gaactaacct gagtgataat ctgagctgtg 22260
atagcagccg cagtagtaat agagtttggt gtactgattt cagcattacc aaccttcata 22320
ccattcttca acatatcttc caaagcaacc aggcaacagt tagtcatgcc caaaagggaa 22380
taatccaagt catggaaatg aatatctccg attgagtgtg ctgtcctgat ctgtataggc 22440
atttcttgca aggttaaaaa tttacttagt tcacctgcaa tcatatcacg ctgagtagga 22500
aaacgttctg aaggtttatt agcattatta tgctttaggg tagagtctgc aaagccatct 22560
gtaataccat agatattatc gaataattca gtttcagtca ttaatatttt cctcaataaa 22620
ttttactgct atatctggtt caatatcaaa aaactctgta gcaccatcaa aatccttata 22680
atagcagttc cattctttaa aatgctcatg cgctgctttc tcaattttac ggcacttagt 22740
atagtctaag atatcaaata ctttaactaa tttaagatct ggtatattag catttctgct 22800
aatttttcct aatctagttc ccggtttatt agagatacca atcttagtac ctaaagcttc 22860
actatacata atatatagtt ttgttttaga cctgccttct tttttacata tcgggcaatt 22920
actatctaaa tttttcactg cattaatagt agtattccac tcatggccat tttcacacct 22980
aaaaagggtc ggagtcctat tatttacata tttgcctact atttgtattc ctttattatt 23040
taattcctgg tttattacct caggagttag tttagctttc ccactacaat atggacatcc 23100
aaatcccatg ttaagtaaca ctgatggttt tgtttcccat agatgcccta ctgaacatct 23160
aaacctagtc ttagtattag catttatata ttctccaact aagacaattc ctctatttaa 23220
aagtctatta ttgacttctt ccgtactcaa tttctttggc attttatttc tcctatttat 23280
tacatagtat aatataaaaa taccactatg tcaataaaat tttttagcat tcttaaataa 23340
cttgacttta atcaattttt gaaaaaattt tatttgccaa cctctactaa aaatagtata 23400
atcacgcacc cgtgtacgcg atataaaatg attatctagt accatacatc tcccaataaa 23460
agcaagcaaa ttttttattt aaatgcaata tcataggctt atttaaaatt gtagttgctt 23520
tctcccgtat atattggtat aatatatttg taagttgata aacaagatct ttttgttgat 23580
cctctgttag cgaagtacac tataggagtg ttatttatgg gaaaagcacg tcaaaaaaga 23640
gagaaccgca atggttcaag aaagcgtggc aacaagtatg agagtaacgt aattcaagca 23700
gatttctctg gtgattacgc caatccagtt gctaaatccc tagttggtaa aaaccgtgag 23760
caaaaatcat atatcaatat gatcaagaac aacacagtga ctgtgggtat tggtgaacca 23820
ggtactggta aaacctttat tccgtccgtt cttgcagctc aggaactcgt agacattcac 23880
tcagatattg agcaagtgat cctcgtgcgt ccgaacgaac ctctaggtaa atctcttggt 23940
atgctcccag gtgatctggc agaaaagcta gagccttggt tggaaccaat agccgatggc 24000
atgaaatggg caatcggtga tcacgcctac aaaggatatg tcgaacgtca gaaaattaaa 24060
ttcctggcta tcgaacatgc ccgtggccga actttcaaca actcctatgt aattgtcgat 24120
gaagctcaga atatctccgt tgaagcaatg atctgtctct tgactcgtgt cggacaagat 24180
tgtcgtctaa ttatctgtgg agatatagct cagaaagaca tcaagggtga ttcaggtcta 24240
gcacttctta tggaagtcta tgagaagtac gaaaactctc ctttctctat gatcgaactg 24300
atcgacaacg ttcgctcacc agagtctaaa gcattctacg atatctttaa ggatatgggg 24360
aaggtgtaat atgggaaacg tcgtacatct gcaccgcaaa actaagatcc accgtacttc 24420
attgagtgct gctaatatga ttacacgtaa ggaaggagaa gaaagtccta agactacctt 24480
agcgtggaaa attgtagcct ctaatccaaa tcggcctttc aactataatg aactgagtac 24540
ttctatagac attcttatgg ctaaggaaat acgacagaaa ctaactgata tgaccgataa 24600
acttaaatgg taagacagac gagcctgggt atgaaaatat ccgggctctt tttatctgta 24660
aaattgtact tgcaaaatga cccaaaacaa agtagaatat ttcctaaatc ctgataacta 24720
caaaaaggaa agccaaatga gccatcgcat cgaaaaagta attaaacgtg acggtactgt 24780
tcaggacttt gctcctgaaa aactcaatga ttgggctgca tgggcatgta aagatactga 24840
cgtaagttgg tctgccatag ttatggctgc cctgaaaaat ctacccaaag gtgttgtaga 24900
ctccgataca ctccaagatg tgttaattaa agccgctgaa ggtcttatca aagacaaccc 24960
agcatatgat actgtagcaa aggaattacg tcttgcgcag atgcgtaaac gcctatatga 25020
ttcctttgaa cctccttctc tgcgtttctt ccatgatcat atggttcgtg ttggcgcttg 25080
ggaagacatg agtgcatgga ttagcgatga tcagtttgat gctctgaatc aagttattga 25140
tcatgatcgc gatcgtttat tcaccagtgg tggactgaaa cagttcttcg acaagtattc 25200
ccgtcgtaat attgcaactg gtgaaattta tgaaactccg cagtttgcct acatgggtat 25260
ggcaatggcg atgttatctc aacctaactg gacaattcta gatgcaatcg acctctacaa 25320
tgcaatgtcg ctccacaaaa tcaacgttcc tacgccgcca ctggttggtc tgcgctctag 25380
tgaccgtgga tttgctagtt gctgcctcgt ggattccact gacacgctgg attcaatcga 25440
cacagccgag cacatcgtct tcaaaatggt cgcagccaga gcgggaatcg ggtatcatct 25500
tgaaagccga tcaattgctg atccggtgcg aaatggggca ttcccgcatt ccggaaaact 25560
gccatattat cgacacattg accgatcagt aaaagctaac actcagcaaa cccgtggcgg 25620
ttctgctact gtgtcttatc catacttcga tcctgaaatc attcagttga tgcaggttaa 25680
gcagcaacgt gctacagacg agaataaaat cgataagatg gattactctc tgagctttaa 25740
caaccttcta ttaaaacgtt acctgaaaaa cgaagatatt acgctaatgt cctacttcta 25800
cgcaccagaa gttcatgaag cgttttatgg cgaagatgag gctaagtttg aagaaatcta 25860
cgtggcagcg gagaaacgtg tggcttctct tacaaagatc gaccacgaag gaaaaacaat 25920
tccagcagct cctagaatct ctgcaaaaga aatcctagat acttggttac gtatccgtat 25980
ggaaacaggg cgtatgtatg ctcatcacat tggtgaatct aaccgtcatg gtaacttcct 26040
tgacccgatt cgtatgacca acctatgtgt tgaaattact cagccaacca ggccattcca 26100
tcacattacg gagctgtata agacccaaga tcaattagat gatatgcgtc cagaagatat 26160
tggtgaagta tctctgtgta acttaggtgg tgttgtactt ggacgcatgg aatctctggc 26220
tgagtgggaa aaaacttgct acatcctact gaaattcgtt gatacaatta ttgaaattca 26280
ggattatccg ttcccaacta tggaatatac ggctaagaaa cgtcgtaatg ttggtattgg 26340
cctgatgaac gcagcaggtg caatggcagc agaaggtctg gcttatgaag gtattgaagc 26400
ccgtaactgg attcaccgtg aggctgaaaa actgtcttac ttcctgcata aagcctctgt 26460
acgcctagct aaagaacagg gtgcatgtga gtggtttgat cgtactaaac catctaaagg 26520
aattctggta atcgacacgt acaagaaaac tgttgatgaa ctagtatccg taggtctaga 26580
aatggattgg gagtctctgc gtgcagatat tctgaaatat ggtatgcgta attctgttct 26640
gactgctcag atgccaggag aaagttcttc tgttctgcta ggagttacta actctatcga 26700
accgcctcgt aagattatat ctatcaaggg tagtgcagtg aataaagtaa ttgcaattgc 26760
tccaggtgca accgattggg aaacgttaat gagctataaa ctggcttatg atgtagatcg 26820
tatcgagtgg atcaaatggg tagcaactat gcagaaattc ttcagtcagt ctatcagtac 26880
gaatatgtac tatgactaca ccaagtttga taatgaaatc attcctggcc cagtagtagt 26940
tcgtgatttc atgactgctg ttaagtatgg gtggaaaact ttctactatg caaacttcaa 27000
taccgcaaat ggtggaggtg ctggagaaga agcagccggt tgtgcatccg gcggttgtac 27060
tatttaataa aaacaaggag atcttcgggt ctcctttttc tttgaaattt tacttgctaa 27120
tcgctaccag atttgctata atatctccat tagttaagga gattagtttt gataacacaa 27180
gatgaattaa aaaggattct ggagtataat ccagatgaag gaacatttac ttggttaaat 27240
aaccaagaag tacacaataa agtaagagga ctacctgctg gttgtttaaa accccactca 27300
gtagatccta accgaaaaga cttagtaata caaatcaatg gtaagaaata tcctgcacaa 27360
aatattgctt tcctgtacat gacaggaaac ttacctaaag ttatggttga tcatatagat 27420
ggagattcac taaactataa gtggactaat ctaagagaag cagactggca gttaaatagt 27480
caaaatagaa aaaccagtaa aaacaatata ttgggtacta agaatattcg gaagaaaggg 27540
tctggatttc aagttagaaa atccattaac ggaaaagaat acgtgaaaac tcttccaact 27600
ctcgaagagg caatagagta tcgagattct ttaatcctct ctataaccaa agacccaaca 27660
ctacttagaa acacagataa ataggagaac taatgacaac tttacttaac ttaaactggg 27720
atcataccaa taaagatcta tttctcggtg attctcttgg tattgcagat tatgtacgag 27780
tagctcatcc agagctagaa aggctggcgt tattagctcg ctctcaattc tggactgaaa 27840
ctgaaattag tcttgaagct gataaaaagc agtggggtaa tctcccgcgt gatattcagg 27900
aaattaccct cctgaactta gcatggcaaa cccaaacaga ttctttcatt agtcgtgccc 27960
ctgaagcagc tattatgcca cttgtaagcc gtcctgaact ggaaggtatg ctcaaacagt 28020
ggagctattt cgaagatctg catagccgtg cgtacagtaa cattatccgt aacgtgctga 28080
ctgatccagc agaatttatt gattctgtaa ccaaaaacca agaagcattc gcacgtatcg 28140
tggattctgt tgaattgttt gatgaattat atcagttagg tcagtatttc cttgcagttc 28200
gtgatcatca gggtgataat acttatccag aaactgaatt ccctgaagtt aaacgtgaaa 28260
ctcaggctaa actgctagat gcttactttg caatctatgg tctggaagca atgcagttct 28320
acgcatcttt tgcatgtacc tttgctctag cagagaatga cattctacaa ggtattgcta 28380
agaacctgca attaatcgcc aaagatgaag ctctacacac tcaaatgtct aaagctatca 28440
tccagattat gttccagcag tttgataaag atctggtaga tgaagcattg gctaaggcac 28500
cagcacaact cctgaaaact ctgaaaactg agattgaatg gggtcacttt atctttaaag 28560
gccgtagtct gattggtttg aacgcagaac tgctcgaaga atatctttac tttgtaggac 28620
gtaatgcatt tatgcacatc ggtgttgaat ggccaagcca cttacctgta atcacgaaga 28680
accctattcc atggattatg aactggttgg atactacgtc tttacagcct gctccacaag 28740
agattcagat tggtgcagca taccgtgtag gtcaagtaac tgaaacctct gctgatactc 28800
tgaaagatct tggcaacgaa tttgagggta tgttctaatg atagcagcaa tctatgcaac 28860
tgggccgaat ggggagttcg gcctcaacgg taaactccct tggggttctt tcaaagaaga 28920
actagatatc ttctacgcta ctttggattc tctagcacct gataatatta ttgtaggagc 28980
agggacttgg ttagccttcc ctgagactct gcaaaaaaga atactaggag attctgacct 29040
atttatttat gcaaatagac cactacctaa ctctgtagga tcagccacac gcataagtaa 29100
tattggatat agtctgcctg atttcctcaa ggaagaagta actgtagtat taggaggagc 29160
aactctgctt cttgaaatgt acgtcaataa tcatctagat atggcgtatg tatctactat 29220
taattcagtt acaccattat ctgctgatgt acatcttcac catcatatta ctatgactaa 29280
ttcaacagca actaggatgg actatgcagt agggcataat gcagacaaaa gtttatcttt 29340
tgtacaagaa ctggtgactt attaatgcgc caatatattg aaactggaaa acgtattatt 29400
gaagagggtg tatgggtaac gaatcctaga acaggggttc gttgcctaac cattattgat 29460
gcagacttta cctttaacgt aggagcaaga gaactgccgc tagtaactac tcgtaaagca 29520
tttgctaaac aagcaataat ggaaatgatc ggatatctcc gtgggtacga ctcagcagca 29580
caattccgcg ctattggtgt taatacatgg aatgctaatg caaatgaaaa tattgcctgg 29640
ctaggtaatc cattccgtaa aggcgaggat gatatgggac gagtctatgg tgttcaagga 29700
cgtcattgga ctaattctga tggtatggta tttgatcagt tagccaaagt ctatcataat 29760
ttgaaaaggg ggattgacga tcgtggagaa attttaactt tctggaaccc tggggaattt 29820
cgtaaaggat gcctacgtcc gtgtatgcat acccatcaat tctctatttt aggtgataca 29880
ctatacctaa actctttcca gaggtctaat gatttcttgc tgggccaagt atttaatatg 29940
gtacaatgct atgcacttct agctattatg gcacagatta caggtcttaa gccaggtatt 30000
gctcgtcaaa agattatcaa tcttcatatc tatgaagatc agtataatgt tctgatggag 30060
cacaagcaat tcgatcgtga accttttgca cctcctactt tagaaatcaa tccaaatatt 30120
agaactttgg atgatgtatt aacatgggta agtaaggatg attttactgt tcgtggctat 30180
gaacaccatc ctgctattgc atatccattt acggtgtaac atatgttaat tatactatta 30240
tttgtcatat tctttctaaa acattttatg gccgattatc cactccaaac accatatatg 30300
ctaagaaaga cgcataaaat agggtggaaa cttccattat taacccattc gctagtacat 30360
gctggattaa catttgcaat tctaatccta ttcacatcac caggaatgtc tatagcttta 30420
gctattatgg aattaattct acactttatt atagattatt ggaaagcaca acgctgtaaa 30480
tatgctataa gcgatgctaa gttttggata gctttaggat ttgatcagtt actgcattat 30540
ctgacatacg ccctaataat cttgattata atctaattca aatagccctt cggggctatt 30600
atatttaaat ttcatttgct tacttagtga aattcaaata taatatactt ctaatttgac 30660
agaaggattt aataatgaca gccatagtag catttaaata taaagataaa gtatacttag 30720
ctggtgatcg tatgggttct aatggctata cgaaactgct ggttaaaaat ccgaaagtat 30780
tcaaaatctc tgatgatttg tacttcgggt acactagttc cttctacatg ggacaattac 30840
tagagcatag ctttactgta ccaccacgtt tagcagatat ttccgatgat aaatatttat 30900
atacggaaat agtacctaaa ttacgtgata tgtttaacca gaaagatttt ggcgaaaaga 30960
aaaatgaaaa atatgctgaa cctaatctgg gacaatttat tatgctctac aaaggaagaa 31020
tctttatttt ccaatctaat gcttctattc tagaagttga atatgccggt ataggctgtg 31080
gaggagaaga tgtactttcc agtattcgta caggtttagc tattgcggat tgtcgagcca 31140
cagatttaga tgtagatgat atatttgaaa tcattgatct ggcttttacg cattgtgcag 31200
attatatgtg tggtgtatct tctcaacatg atgttttagt aattggagaa taaatatgtc 31260
tattctttta tctgctttag aagcaaaaca aaatactatt gctagcatta aaaaggaagt 31320
aattgaccta gaatcactta ttgccgtaga gattgacaaa gctattaaag ataaagaatt 31380
taactgtcac atccagggat taactgaata ctcagaaaac gctagaaata gcgtcatgca 31440
aaaacttagc aaactaggtt atgttgtaga agctaagtat gaacagcgtg aaggtttttg 31500
gcttgaaatt gattggagca aataatgtcg gttttccaca tatatacaga tggtgcatgt 31560
aagggcaatc ctggtttagg tgcgtgggga ttcgttgtct ataatgataa tgatgatcgt 31620
attggctcta aatctggctt cagccctaaa actactaata acgaaatgga acttactgca 31680
attgtagaag ttcttcgttg gtctactaag aaagataatc gtccagtaat tatctacact 31740
gattctgctt actgtaaaaa tggtatggaa agctggatgt ggtcttggca gaagaaaggc 31800
tggaagaagg gagatggtga aactcctctc aatctagaac tctggcaaga agcatttaaa 31860
ctaactcaac agtatattaa ctttcatgat accaatccaa cctttgttaa ggttaaaggt 31920
cactcaggta ttcgtggtaa tgaagaagtt gatgctctat gcaatactgt aattactgag 31980
caagaacttg ctgagatgta ataaataatt taaaaattca cttgcttaaa gccctcgaat 32040
ttagtataat attcgtattg aaagtgagga gggctttatg cgaatttaca actcaaattc 32100
attaggattt aaacctttga gaaaacgcgc acaatctccg cgtcagatca gaaaggcaaa 32160
tattggtgag ccagaaacta ggcttccacc acctccggag caacgtctag tttacctaga 32220
tgaagaatta gccgaaaggg aacgcaaagc tcaagaagaa attgagcgca agaaactgtg 32280
tactgctcct gcatataaca agggtggtta tcagtacgtt tccagtgagg aacaagctaa 32340
gatgatcggc agataatttt taaaaaattc atttgctaaa cgattaaaaa tttcgtataa 32400
tatatctcat aaattgatca acaaaaagga aaacaaataa tggctaagca gaaaaacaca 32460
aaaactcagg cagctccggc agttaaagct ttcactcaga ccgaagccaa tcgtaaggct 32520
cgtctggaac gtcatctgcg taagcatcct actgatgctc aggctcagac tgccctgaag 32580
tctccggctc cggttcgcca gaagccgaag gccaagaact ctactcgcgc aactgctaag 32640
caggttgtgt tcatcaaggg tgagggtcat aagtctgtac cggtaactct gagctttaac 32700
ggcggtgctg agatgttcgc ccgcaacggt atggctctga aagactacga aaaagcagtc 32760
aatcagaaac gtaagccaat ggctgacgta atgcgtgaat cccgtggaca gtttggctcc 32820
gttaaaccga atatcttcgg tgtagagtac agccgtgaca acgttcgcgc tctgtgctat 32880
ggtatcggta ttaagtttac cggtgcttct gatcgtaaat ccgctaaacc tgctcgtaag 32940
cgtaaagcga agtaatgatt gatggggcga aagccccata tactagctct ttctctgatt 33000
aatcaaggat attttatgtt ctctaagaaa ccacgttccg ttactgaaat cgttgcttcc 33060
tttaccacta ttactgatga acttcaagct cgtattgaag ctgaccagaa aactgctgct 33120
gatattcaga aacagcagga agaactcgct ctgaaactgg ctgaaaccaa taagagtgaa 33180
aaatctgctc aaactattaa ggaaaatatc cttaaactac tgggcaagta atttaaatca 33240
gggggcgaaa gcccctctta tttggagaaa ctatgcgtaa cttcgttgca aagaatgatt 33300
ttaaccgtgc tagcactcat aagtctgcta gagactacac tcgtctttct aaacatgagc 33360
ttctagaaga aatcatggaa gaaaactggg atgttaacga aaatggaaca gacctgtttg 33420
aagaagagga aattcctctg aaagacccca aacagtggga gtctattaaa gagacagctc 33480
tttaatagtt ttctacaaca ataaaggaca taattatgaa attcaagccc tttggtatgc 33540
tggacattga atctctaggt actccaggaa attgcggtac tacccatatc gcaatgcctt 33600
ctttcgcttt tgtagcgatg cacggaattg ataaagatcc tggtctggta ttcgttactc 33660
tagacgttca agaccagctt aatgcaggtg ctaaagttac agcgtctacc ctagctttct 33720
ggatggatca agctaaaaac agtccatctg cgctccatat tatggaggct ctaaatggtc 33780
gtaatcctaa actgattgcc ttccgtgatg gtaagcatca ctgcactaat gacgtaggct 33840
ctaattatgc tgccttctct atggctcaga acattatgga attgtctcta ggagataatg 33900
ctctgtatta cggcaatggc ccagagtttg atatgacaat ctactctgca aatacgttcc 33960
atgcaggaac ccatgaagaa atagttccgt ggaaattctg gaacttaggt aacgttcgat 34020
ctttccgtaa tcagtggatg caagctggtt atagctataa agatctagaa gccgaaggtg 34080
ctagctgggc aaaagctaaa atggaaaaga tggacaccat tcgttatgga atctatcctg 34140
tgaagcatga tccagcattc gatgctttag ttgaaagtta ttgtgtagcc atcatgatcg 34200
aaaaaatcaa aatttgattt gctttcagct ccaaaattct gtataatata ttcataaatt 34260
tgagagaaca acaaaaggaa aataattatg ccagtatcta aaaacgtccg taaaaatggt 34320
aaagtagcaa aacgtaacct gggtctgcgt cgtatggctg aacgtcagtc cggcatccag 34380
aaaatcttta acatactaaa tcgcgccaat ccgaatattg ataacagttc tgatactgtt 34440
ctgcgcactc tgctggcaat cggtctgtaa tagaccgttt ttaaaacaaa ttcaaatctc 34500
aaacttcata taaggaagca aataatgact actcgtatct ctaaaactcg tgctctggca 34560
actatcaagt ctctggaagc taaaatccgt aaagctactg aacaacagct tttgatcgct 34620
gttggtgaag gtaaggacaa gaaccaagtt gtagttggcg ctgcaatcga agtagatgct 34680
ctgtctactc gtattaagac tgatttccag tctctactgg atatgatgac ccagcgtgat 34740
catctgaaag cagcgctgat caagagcaat gctgaaactg ttgtagagat cggctctcgc 34800
tctatgactg ttgcggaagc aatcgaagct aagcgttcta tggaactcaa ggctcagttg 34860
ctggctaaca tgcgtaagca gttccatgct gctactgtta agtttaacac tcagaaggct 34920
cagtttgatg ccaagtatga gcgtttgcag gataccatgg ctacccgcga caagaagact 34980
tccgaggatg aagttaagat gcagcttaac ctgctggaac tcaagaacac tccgttcctg 35040
atcgatccgc tggagctgga aaagctgatc aagcaacacg acgaagaata ccaggacttt 35100
gcaactaacg ttgactttgt tctgtctgag tccaatgcct ctacttttat cgaagtagag 35160
taataattta aaatactagt aaatcggtag acgaaagccc aagcatctta cctcgcagca 35220
gtggttgaaa tacgaggtta aataagaccg ctgaccatac tagatacggt ataatctatt 35280
ggagattatc ttaatttatt tttcggcaga tgttgcatct gtcaaaataa aacaagacgt 35340
cgttggtagt accaacacaa cttcaaagat caacgctcaa gtatgaaagc tgaaaagtta 35400
aaagcactca aacttctaac cttctaaagc taaaagatgc aaagtattcg ttgatgattc 35460
aaaccctaga tcaaaggtac atggctcata agatatggcc tgtggcgcct ctaggctgtt 35520
tatcggttta ctagttctct caatctaaaa atgtatttgc aaaacagttt aattatctgt 35580
ataatatatt tttaaattga tgagacgagg ctattatgat ttttcatatt ctgattgatg 35640
atgtgcgtaa ccttcatgga atggacatta tcattcgaac ccccgaagcc gctgttgagt 35700
ttctgaataa aaccgatacc acaggtcatt tcgtttatat ggataacgat ctggctgtag 35760
atggtatgga aggttatcaa attcttcgtt tactactaga atttggtcag agacccaaga 35820
aagtggtact agtaacctcg aacccagtag ctaaagagac catgcgtaat gatttgcttg 35880
acttagggta taaagaaaat cccaatcgtg ttgaatatga ttggcaggag taaaaagaat 35940
gaaggcagct ttgcttttag tagctgccct taccttcagc tttagtagtc aggcatcgca 36000
cgatgcaaaa gaaatagatt gcattgctaa aaatatctat ttcgagagtc gtggtgaggg 36060
cataaaggga atgacggcga ttgcacatgt tactaaaaac cgtgtaaact ccggtaaatt 36120
ccccgattcc tactgtaaag tagtatatca gtctaaccag ttctcttggg tggccaggag 36180
accaaaagtt gataaaaccg atgaggcttg gcaagttgct aagaatttag ctagagtgat 36240
atactacgtt gatttaccac aagatccaac acatggagcg ttatacttcc atagtggtaa 36300
agataaacct tactggacaa agaaatttaa gaaaacaact aagattaagg gacatacatt 36360
ttataagcct gttgtcacta aaacttaaaa atttattaaa tgaaaaaatt aacacaagaa 36420
gaagtagaga gttacctagt tagtagaggc ttattcacta atgataagta tactaatact 36480
aggactaaaa tgcagttctg ttgcacctat ggacatgttt ttaatgctac cctaaataat 36540
attaggaacg gaagaggttg tccctactgc tatggaaatg ttaggtacac tcaagaacaa 36600
gtatctgaat atttgcgtag tttaggatat tacaccatta ccaaatatat aaataataac 36660
tctaagctac aacttacttg tagtaaaggg catgtatgga ttaccacttt tagtgccata 36720
aaaaatatgg gtaataggtg ccctgagtgc tccaatatat ggagcgatgg tgggatactt 36780
tatataataa ctagttcatt aggtacaaaa ataggaatta ctaattctcc tgctcgtagg 36840
ttaagggatt tgattactgc atccggggat cccacagcta agtattatgg ccactataca 36900
tatggtagct cttctaaatc agaaaccctt gctttagaga agaaggcaca tatgtatttt 36960
aaaagtcata attgtggtta tagtggattt gctggatcta ctgaatactt taatatttcc 37020
cccgaagagg cagaagaatt cctcttgaaa gagggggcca caatagtaca gaaaggggta 37080
gtttatgaag aacttgatcg tcattggtgt taatactcgt ggtatccgta ctacgaaaac 37140
agttaaaact actaacgttg ctgaaattac tacaaaccct aagaagtttg atttcgctaa 37200
agtctcggca gttatgacag aacaatcttt taaccaagca ctgggaatta aataatggaa 37260
gagaatattc tagatgtttt gcgtagggct ttgcaggaat ctaaggttga tggttctgat 37320
aaagaagtag ctaaaagcta ccagaccata atcggagatc ttcagcgtgt tgataaagac 37380
ttcattactt ctgaacagtt tgtatcttat ctaaaagctc aactgaaaag cattaatcag 37440
acgaaagcta aacttcacgg tcaagacctt gataattata gcttgcagtc tgctcagtat 37500
gaatatatac tgaacaaatg gttacaacag tatctgccgc ctcagctttc tgattctgag 37560
attcgcaaat actttgcaga attagttaag ttgaaccctg ggattacgaa gggtatgctg 37620
atgaaagcga ttaaggaaga attccctggt agttatgatg gtggtattgc tgctcaagtt 37680
gctggcgaat tcaataaaaa ttgatttgct aaacgcttca aaattctgta taatagattc 37740
atgaaaagga aagaataccc tactaaagaa atacttgaaa aatattttgt agctgatcct 37800
gataatggga tactatatag gaaaacaaag aaaggtttga aagagtgttg tgccaataat 37860
aagaaatcac atccagatgt gggatttcag ggcgacacct atcaactgca tgtcttatta 37920
tttattatgt actatggata taggcctgaa gaagtagatc ataaggatac taatcctaga 37980
aataatagga aaaataatct aagggacgcc tctagatcgc aaaataatgc gaatagaaac 38040
caacaatgca ataatacttc tgggtacaaa ggagtaagtt gggaaggctc ttcctggaga 38100
taccaagtaa gacatgaagg aaaaaattac agtaaaagag ggtttaaaac tgcggaagag 38160
gctttcgagg cctctaagat attaagagaa tccttacata aagaatttac taactttggt 38220
tagtaatagt ggggtgtcga aaccacttat gtaacaacac tgccgtcctg ggctgcctgg 38280
ataagccagg cattgagtcg atgtaggttc gagtcccacg gtcggaaaga gttggataac 38340
tggtgcttat accggataag cactcgctgc tggagtacat actatgacgg ttcaggaggt 38400
acgccgcctg atcagtatga ggttagaatc gaaacagcta aaccacgaaa ggactgggat 38460
gcttgctgag ctacccctac ctgatacatg cctcagctaa cttcgcatcg aaaatcggag 38520
gtgcgattta accagaccgc gtaactatga gagtgggaaa tggtaaactg tatagcttga 38580
ctctaactta gttaggtggc tacagagcct taatgggaca gtctagatac ttagtgaacc 38640
gcggataacg ctaagaatac tgtgaaaata ctatgggagt catgacccgg atgttgcccg 38700
atcgctatag tggcgaataa ccacgaagtc accttgactt aaactggaag atattgttcg 38760
acggaagggc tgaagagctt aatctgaggg ggcttaccct acctaccgcc gggtatcttt 38820
aagagttctt acgagagttc ttaaacatac ctaaacatta aggaatacta atgaatctag 38880
gaactacttg tatatttgat cacagagaaa tgtgttgccc acatatcctc tacgtagttc 38940
actttgcaga gcgtcttacg ctggatgaag ctcgtgagaa aatgagtgga attactggat 39000
atgacttccg tgaaggtgaa tactttagcg ttgctcttga ttacattgac aatacccgtc 39060
tttatcatct agtgcagcta ggggaagaaa ctgatgaagt gaaaatggcg aaagatactt 39120
tacgtcgtta tcgtcctcag attctagaac gtacacttcg tggtgagttc agtgacccat 39180
tctttttgaa gtgttatgat gcagacgcta atgagtactt ggggcgacaa aggttttacc 39240
aagaaatcaa agagtttgtg ggtgaaaaat acgaagcact actcgttgat tctagagcac 39300
gacgtgcagc taaaaatgct gagaaatcag ctaaagcagc actagaaagt caaaccaaac 39360
tgaacgaaat cgttaagtga tctaatttcg gggtgtagtc taagggatag gcaggagtct 39420
tctaaattcc tttatgcagg ttcgaatcct gtcacctcgg ccaattttat taactgctag 39480
gaaaataaag tgaaaacacg atatatagta aaagtagtag gtgaacattc acaagtaaat 39540
accaccgatg ttacagcact agcatttgaa gtagtctccg gtatagtcat atttcataat 39600
ggagatatca aagctcctga atttgctgtg gattccagcc tgtttatttc tgcaactaaa 39660
gtaactgtag tagctaactc ttctaaataa taaggaaata tatgtttact aaaaacacta 39720
aagaactgac ttctgatcaa aaagtaattc gtactattaa acgttggggt attggtgctg 39780
tagttggtct tggtgccctt attctggccc tcaattccta tacagttgtt caggacggta 39840
cggttaaaac tcaaactttc ctgggtaaag ttagtcctaa tcctgtattg ccggggttcc 39900
atattgtcaa cccattcgct tcttttgata cttttagtac gaaagatatt tctatgaaac 39960
tggataaact tcaggttcct agccaggata agtttaagtc tacagttgac attaccgtta 40020
tgttgcagtt tgatggtgct aaagctccta tgaaccgtgt taacgcaggt actcaggatc 40080
aagctctgaa caagtacgtt gaagagaaga tgctgtctac gattcgtgag tttggtaagt 40140
ctgtaccaaa agcacaagat ctgttcgacg ctaagattca ggctcaactg caaaccgcta 40200
ttcagcaaga agtagaagag tacgcgcgtc cttatggcta caccgttaag caagtattcc 40260
ttcaggatat tactctgcca ccggtaatca tggaacaggt gcaaaacacc aaagttcgtg 40320
aagaacaagt taacgctgca aaagctgaac tagctcgtgt tgaacaagaa gcacagcaga 40380
aagttaagca agctgaggct gatcgttctg ctcgtgaaaa tatggcagta gctaacgaac 40440
gtgacgcaga tgctaaactg tatgcagcta agaaagaagc tgaagctaac accgctctgc 40500
agcgtaccat cactccagag atgattcgct ggaaacagct ggaagttgat atgaaacgcg 40560
ctgaaaaata tcagggtggc gttcctcaga ctgttgtagg tactggttat gatggccaga 40620
tgattatgga tatgcgtaat aagtaatagt tacacgtaat cttcgaaaca agcccgcgtt 40680
tcctccgaga gcgcgggctt ttctgtattt aaggcaaaag aaaagcccat aagttattca 40740
actaaggtct ttagtattta attctttaga atcatatact attattgcag caacaacgtg 40800
ctacccttaa gtgccctgcg attttcctct aaaaagttaa aaaattcgat tgcttgaatg 40860
ctaaattctc tgtataatat atttataaat ttgagagagg aatcaaatat gattaagact 40920
ggtctagtaa ttggtaagtt cgcaccatta acacgtggtc atattaacct aataaacaca 40980
gcagcaacta tgtgtgaacg agttatagtt gtagttagtc atgataatcg ctggctggat 41040
aaacaaaaca cacgtgatca ggaagtctta cagttaaaga accgacttcg ttggttagaa 41100
caaacttacg cggatattga gcacatttcg gtagagtaca ttattgaaga tgatattcct 41160
gaatatccga atggttggcc ggaatatgct agaatcctta atgaaaatta tggacatttg 41220
cctgagatca ccattttctc tagcgaacta gactatgatg cagaatataa gaaatatctg 41280
ccgaagtttg gtcattatat tgtagattct gatcggactc gtgttccaat ctcagctaca 41340
atgattcgta atgatcttat gaagtattgg gagtttcttc ctagcgtagt tcgtaaagat 41400
tacgtgcgta aggttgtaat cattgggact gaaagctcgg gaaagactac tttaacaaaa 41460
taccttgcga agctatacaa cacttcgtgg gtagaagaat atggccgaac gtattgtgaa 41520
gttgatctag ttggtaacga aaatctactt accagtgaag attacagtaa aattgcgttc 41580
agacacaaag agctagagtt tgaagcagaa cgtactgcaa ataagatcat gtttgtggat 41640
acaaatgctt ttattactga gttctaccac agactttatg aacatagtaa gaaccctgtg 41700
gttacagcaa ttgcaaaaga agaacattac gatttagtaa ttgttctagc gcctactgtc 41760
aagtgggtag atgatggttt acgtattaac tccgatcgtt caattacttc tgaactgttc 41820
gagcagatga agaaagaatt tccaaatcag ttccctgaag ggcgtactgt ttacatcgac 41880
tctccggact acaaagttcg tatggacgaa gcagtaaacg cagtagactg gctgattcat 41940
cactttaacg ttggagagtt ttaatatgac taactatctc gtaaatgact ttaaaaactg 42000
gaaacctttg gaatatctgt ggctgtttat agcggttgtc ttcattagtt tagcaaacta 42060
taatggtaca gctctagatt gggctactgc aataaccaat gtggtttgtg ttattctggt 42120
tgcaaaagga cgtgtttcca actatatttg gggcttagtg ggtgttctta catatggtat 42180
tacagcttta aacgcagatc tttatggtaa cgctgcacta aacctgctat actacgttcc 42240
tatgcagttt tatggaatgt acttatggca taaaaataag ccaagtaatc tgccagatgt 42300
tccaactaag gttctgacta aagcccaagc tatgctgact ctagggattc tagcagtagc 42360
cacgttaggg tatggtagtt tcttagctat tacggctgat ccatttccaa tgttggatgc 42420
atttactgct gtaggtagtg tgatcgctat gtggcttatg gtcaaacaat atgctgaaca 42480
atggctgatt tggattgcta ttaacattgg tactgtgtac atttggcttg taactgctca 42540
atctacaggt gcatcttacg caatcctagc aatgtggcta gtattcttag gcaactctat 42600
tcttggagct tataaaggat actttgtacg cgttaacaaa taaagtagtt atagccctct 42660
aagcgagggc tactatacag aaattttcac ttgcttaata tagaaaattt ctgtataata 42720
tatttataaa ttcgagagag gaatcaaaca tgaaacgcat tgtacttgta ttgactctag 42780
tatctacttc tgtatttgct gctccacaac aggaactttg tcaagttcaa gtttgtaata 42840
agctagaacg atttactcta gacatttggt cccacattaa agataatatg ggtgaacagt 42900
gttttgatat tgttctgcca aaagaacaag ctgttcctgg ggctgttctc agctcagaat 42960
cacgttggtg gcaaggttct tctatcaacc caactaagaa gtcagtgaca cgtatcaaac 43020
aagtatatca gtgcaacgtt cagtaaaatt tcacttgctt aatgccgaaa ttttctgtat 43080
aatatattca taaatttgga agattaggaa ctatttatgt tagactatga gtctatactt 43140
tatagattcc gtttagaaga tggtgtacta gtgtggaatt ccctctctgg aaactctaga 43200
gaggagaaga tatttaatac taagtttgcc aataaacctg cgggagcttt acacaaaagc 43260
tctggatata tcagagttaa cgttgatcac caagattact tagcccatat catagtattt 43320
atgttgtatt atggatatag accagaaatg gttgatcata aagactgcaa taagttaaat 43380
atccatcctc taaatttaag agcagcagat agtaccacta atacgcataa cgctagtaaa 43440
tcttcaagaa atactagcgg atttaaggga gtatcctacc acaaagcatc tggtcagtgg 43500
caaggtagaa taatgtttaa aggtaaatca tattccgcag ggctgcacag tactcctgaa 43560
gcagcaaatg aagctctaat tcaactgaga aagaaacttc atggagaata tgcccgaaat 43620
aattaaggaa gaatagcata acggtattgc agcagattgc taatctgtcg gtttgaaata 43680
tagccttgtg ggttcgatcc ccacttcttc ctccatattt ggttctcaag ctcatttggt 43740
atgagccgtc gcctcataag cgaaaggtag gtaggttcga atcctccggg agccaccaat 43800
taactaggag actatatgtc tagattctat gaaatgaaga aagcattaga agaatgtcgg 43860
tgtaaggatt gtcatggtgg tggtaggcag gatgatgcag aacctgggga tattttctgt 43920
agagaatggg aatgtcctac atgtaaaggt acgggtatca accctgaata tgcaatttgt 43980
ctagacattg agcgaggcta agatggatat ttttactact ccggcaataa accttttagg 44040
tgttggcttg ttccaagcta cggtgtatcg tactgatgat tctactgatg tggtaacgtt 44100
catcgtacct gagttcttcc tagaaaagtt ctttgaagag tttgagcagt tccgtgaaga 44160
acacaatgct cattccgata tggaagatct agcagctatg ttccctgcgg tttacggcta 44220
tatctttgaa ggtaatggcc tagatcttaa taaatctgaa ctcgttgaac ttaactgggg 44280
catcaatttt gaagttggct ctccattccc tcgatatttc cagggtttag agattcgcta 44340
agattacatg ggggtgtaat cgaattggca taggtactgg acttaaaatt caggttttgt 44400
gggttcgaat cccaccaccc ctaccaaatt aaggaaataa aatgaaagct attaagaaag 44460
cactacgtaa atttatgtac tgtcgttttc atagcatcaa ggcttcaact aatggtacta 44520
tttacgatcg ctatgatcct aagtttggct tacattctaa ataactggag aaaacaaatg 44580
gctaatcctg aaattacggc acaacgcttt atgctgaaag gaattattgc tgaagcaggc 44640
atgacagaag aagttgcaga ttttcaaaag caattcctca atattctcac tcaagccaaa 44700
gagctaggag agaaagaaca tggcgcggct attatggcaa tcacattggt tgggcttgat 44760
ctggctgaag aatcaggagt ttaactcctc caagcagaaa ttttaaaaat gtacttgctt 44820
tttacttaag atttctgcta taatagttat ataagttgat gagaggaatc agaatgttaa 44880
aacgttttgc agcaactggt attgaaagct cagtatgtaa atcctggggt ggttgggaag 44940
gtgagatcga gtggctttat ttctacaatg tagaacttct tccagagatc aaagctcaat 45000
gtattgaggc tggtatggct cctgatgcta cggctgatgt tgaaatcagc atgaataagc 45060
tagagggtcg agtagtcact gttactgatg aaggtgaaga ggtattcgaa cttccattca 45120
ctttgaaagt cactccttca tttgattaac tgatacagca ctgagcaagt tctttaaaat 45180
ttgacttgct caagtgcctt aaaacttgat ataatagtta tctaatttgg aaaacatatc 45240
tgaatgagaa gtaactaagc gagttgcttt taggggtaaa gtcaggaaat cctgaaatat 45300
tccaccttga gtttaaccga cttacttttc attcaaatat gttggattag tatcgtagag 45360
gtagcgaagc agactgtaaa tctgccgact cggaagggtc tcgggtggtt cgactccatc 45420
atccaacacc aaatttaagt cctgttagac aaactggtaa agtcactacc ctttcaaggt 45480
aggatttgcg ggttcgatcc ccgcacagga cgccaatttc tgcattccta gagtgttact 45540
ggacagcatg tcggtctcca aaaccgtacg gtctaggttc gagtcctagt aggtttgcca 45600
atatttgcac ctttagctga gatggattag cgcttgcctg aagagcttga gaggttcgtt 45660
caattcgaac agggtgcacc aatttgaagg agatgctgat gcatcctgaa aacttcttta 45720
tttactgtgc ggataagtca ctgaaacgca gaattaacaa agtctatcat tatcgtgcca 45780
gtcaacagcc ttacgtttac gcttttgacg gagatcacat tccactagaa cagttggcct 45840
gtgattacat ggtattgaag aactttgacg agacaaagat ctcaagcgtt cataatcgac 45900
agaacccgtt aacgtggaag tataagaaga aaaagaaagg catcatattt ctgaaaaatg 45960
tagttgctta accgcttaaa gttctgtata attattacat aaattgaact ggagaagcaa 46020
catgacagaa caagaactga aagaacgtaa gctggtaatg cttcgcttac ttaacatttt 46080
ctctggtatg gatggtggta ttgacttcat tcaactacgc actcttgtag aagaagctga 46140
aggcgaatct attactaaaa tccttaaaca tatgggttta ttactagacg ctgctaatgt 46200
agctactgaa tataaattag aagatctacc ggagcgtgtt tcatgaaaat gttagttaaa 46260
tttaagtgtt gtggttctga gcatacattc actcgttatc gggagtttaa gatgtgtgag 46320
tgtggtaaat caggctacga tgctggagat ggttattatg ctagatctat agggcattcc 46380
aaagacctag aatttactaa tctcgaaaaa caagataaat cgaccgttgg ctgaatggct 46440
taggcgaagg attgcaaatc cttttttatg tgagttcaaa tctcatgcgg tcgtccaatt 46500
tgctgaaaga attgttaaat caattcaggc acgcgtagga acactgcctc agtaattgaa 46560
agatgaggca cggtttggag aggtatgccg ctacacgcta cggtgctaac tccgtctagg 46620
tgaaaatcct agtcatcaat ataaatttag ggtcgttggc tgaacggttt aagcgccgga 46680
ctgttaatcc gtatccgcaa ggataaagta ggttcaactc ctacacggcc cgccaatttc 46740
actaacaact taggaaatag atatgacaac tcgtgcaatt gctcgactag aagctgatcg 46800
tgatagtctg attaataata ttaaggtttg ggatcaagaa attgaaaacc aaaaactgac 46860
attaattcaa gatatgcaac aacagaatgc ccgtcgtaaa gctctaaaag aggttgagga 46920
agctatcgaa aaactcaagg ctgaataaat tatgcacgga atatccttac ccattccagg 46980
gtttgaggag tacttagtaa gtttggatgg gttagtattt agtatcaaat caaacaaaat 47040
actaacccca ataaccagta attacggata cttatctata cgtctccata aaaatggtca 47100
acagagaaat accggtgtac ataggatttt agcatgtgca tttaagaaat taccaagtct 47160
agactctgaa ctagaagttg accatattaa cacagacatt acttattggg gtcttaataa 47220
cttacaagtg ctcacaaaag agcagcatct agaaaagact ttaaaagaca aaggatttag 47280
taaactaaac catgataatt gttgtaagga ttgtggtaaa gctatttctt taaaagctga 47340
aaaatgtcag gagtgctctt cacataaacc aaaagatatc tctataacta aagaaataat 47400
cgaggttaca gtaagagagc taggctggtc aggtgcaggg agaaagttaa gttattcaga 47460
taatgggttg cgtaaaagat acacttcttt aggaagcaat cctaaagaac ttaaaaagtt 47520
aaaggtacta tagtctaacg gttaggatac tcccctgtca cgggagaggt agggtttcga 47580
ttacccttag taccgccaaa ttatacatcc atagctcaat ggttagagct accgcctctt 47640
aagcggaggg ttctaggttc aagtcctagt gggtgtacca aatttaaaga gtaaaggcta 47700
cagtaggtgc acccttacgg attgctacac cggactcttt atcaaacatt ctagcgtggt 47760
ggagagggga caccagcttg ccgagggtag aagctcgttc gattcgagtg aaagaatggt 47820
tctcgtaaat ccgcacaagt gatagcaggt tcaagtcctg cctagattga cttataagta 47880
agcgaaagtt tacttattga gtaggttcca atagatcggc gggtctattc tggcatttaa 47940
gagaagtctc gcaaacttct ttgtcagccg tggggttcat agcctccagg tggcctacgc 48000
aataagtaaa tttattaaaa actcatttgc tattatactt aaatgtctgt ataatatatt 48060
ttataaagtg agttaacaaa gaatattctc cgattagctc aattggctag agtacaccgt 48120
ttggggcggt ggggttgaag gttcgagtcc ttcattggag accaaataat tgcgggttag 48180
atctctggta gagatcgcta gtctcataag ctagaaagag gtaggttcga ttcctgcacc 48240
cgcttccaat ttaatagaag gatttcgaaa tgaaattatt tcgtttaaag gtaagtgatt 48300
tacgttctag caaagatcgt aagaagtatc cactagcgca ctacggtaaa gatgaaaacg 48360
aagtgatggc ggaatttggt attaatcatc cttacttgaa agtctactct attaaggaag 48420
tttatgctta ttaacagtgt acttcaagcc ctggatcttc caaagcactt tactattact 48480
gactccttaa tacgtaggta tatggagaaa tataacgaaa tatctggtaa agaaggtaaa 48540
ctaaaatcct cagagtttaa gttctgctat agaatggcag cgttaaacct gctgaggtat 48600
aaaagggagc ataaaatcac tataacagaa gggtttgtat acgtaatagg taacccagca 48660
tggcctggac tctataagat aggtatgaca gtagacgtaa atgaaagact gtctagttat 48720
caaacatatt cgccctatcg tgattacaag ttgtaccatt ttagattctc ctcggacaag 48780
agaagaactg aaaaagcatt acataatctt ctgaaagact acaaagtatc aggagaatgg 48840
tttgaagtac ctctgttaga cgctttcaaa ctattagtag agaatttgga ttgctagctc 48900
aactggataa gagtagcgga cctttaatcc gtcagttctg ggttcgaatc ccaggcaatc 48960
caccaaattt ttgctcggtt agtttaatgg gagaaccccg tctttacacg gcggttgcga 49020
tagttcgatt ctatcaccga gtaccaatta actcaaaata aggaattgca aatgcaatta 49080
tcaagcaagg ttcaaggcta tttcggtcat gtaacaatgg agttcgctat ctctaaaggt 49140
tgggatttcg agcaagatct agctgagttc tacaaagata ttgatctgaa cgtaccatac 49200
atgggactct ccactggtca agagatcaca gaaggcgatg tgttcaaggc aagtcaaggt 49260
gaggtaacac ctagttttgc caagttctgg ttaggactta ctttgaagga agatgcacca 49320
gaggatggtg tggttatgca gagtatgcac tctggaagct ctgattacct tttcgttagt 49380
gacgttaagg aaatacaaga aatctgcaaa aatgtacttg cttgatgctt aatttcttgt 49440
tataatattt atataaattg atgaggagaa atcctcatca aagttcacta aggtattagc 49500
tcgacctagg actggctgcc aaaaagcacg gttgcaatta gacccctaat ggtgggggat 49560
actttgcaag aagttctaga ttagtccact agattcagcg gcaactgaat agtgtatcgg 49620
gtttgtgaag agcggttatc ctctactggc taatgtctga gggtaatacg cacaccgagc 49680
taatagcctt agtaaactta aagattttgg ggaatgggtc tgcttggagt ggacacctcg 49740
cttgcaccga ggatatcaga agagttcgaa tctcttattc tccaccaaat tcggggtcat 49800
aggttatttg gttaaactta ctgccttgca agcagtggaa ctcagttcaa ttctgagtga 49860
ctccaccaaa caaattaagg aaataatatg gtattcttag cagcctctgt agttcttgca 49920
gctttagcac tagctatcta ttttagaact attaaagatt tagctaaaga aaagaaaact 49980
ttctttactg attattcttg gggcgctcca tatactgtcc gctttatcag cttagaagct 50040
atgctgataa tggtgttcag tggcgtaggt tccattgttg cgggattatt atttatttct 50100
aactaaataa aatggggatg tggcgaaatt ggcagacgcg ctagatttag attctagtct 50160
tcgggtgtgg gttcgagtcc ctccatccct accaaacatt ggattaactc atgatgaaaa 50220
ctcctataac tgatatctgg ttagctgtta atacattaac taaagagtat gtgtttatag 50280
agtatggtca gaggtatgac caaaagatgt ataatctcat acttccagta tccgctaatg 50340
aagtttatga tttaatcctg agatctacgg aagagcaaat cgattaggtg acgaaaaccg 50400
cttggaaagt ggctgactgg taaaacggct tgagagttcg aatctctctt cttccgccaa 50460
acaactggaa ggtaggacat agtggtatgt aacaggtctt gaaaacctgc ccgctgtagc 50520
gatatggtga tggttcgact ccattacctt ctgccaaatc ttgaagtatg ccggaatcgg 50580
tagacggggc ttcctaaagg gcgtaagggg aacatccact cctcataaga tacccgaaaa 50640
cgaggctgag ataaatggtc tatctcatga aggttcaagt ccttctgctt cttgatattc 50700
aatggctcaa agtgtaacgg tgctatttga tgttcttagt aagactttcc cgacatgcgt 50760
tcgggagtaa gctgctaagg ctagagtaga agagtttcgg agagccgcca caattgagta 50820
ttactagaga gcattcgcaa gaaagaatca ggagaaaaac ctgtgcgcag aatgactaac 50880
tttggtacct tagacggcca aatggaggac tgatcatccc ctaatagtat tcaattgtgg 50940
ctatatcata attggttaat gatcctgatt gtgaatcagg cctatgtgga ttcgaattcc 51000
actagccacc ccaaatattg cgtccttatt tcaacggaaa gaatgtaaag ctacgaactt 51060
tacgatcggg gttcgattcc ctgaggatgc accaattaac ttaaacagga aataaaatat 51120
ggctgatttc tgcaaagaat gctctattga tatgtgggga agagatactg gtgatctttc 51180
tggtctgatt acaaaagccg aagttaaaga aggctatggt gctgttgtaa tctgtgaagg 51240
ttgtggcatt atccgtgttg atcataatgg taaacgtcta gaagagcctg atccaatcgc 51300
cacggttcct gtagaataaa aaattattgg agtgtagctc aactggcaga gcgacggttt 51360
ctgacaccgt aggtttctag ttcgatccta gacattccag ccaaacaatt ggggattagc 51420
ttagcttggc ctaaagcttc ggcctttgaa gtcgagatca ttggttcaaa tccaatatcc 51480
cctgccaaac aataagcggt taagcacacg ggttgtgccg caggccttcc aagccccgct 51540
gagtagggtt cgattccctc tagccgctcc aactttaagg aaacatttat gcacgttttt 51600
gagtttcgtg ataaaagtaa caataaacga gttattgtta ttggagaaac atacctagca 51660
tacgaacaaa ctgacggctc tgttatcttg aatgatgaga aagcaaattt tagcttttat 51720
ccagaagaac cttatgaaga gtttcgtact aagtttcttt taccagactg gctagcagta 51780
gccgagcaac tagaatccga agatggctga taacctgaaa ctaaaggttg acaaacaaaa 51840
gaaacgtgta gagattgagc ttggtgacga acgcctagca ctatccttcg agtttcttca 51900
gcagttggcg atgacagtaa tgtctgataa gtctcctgta tttgaaaaag aaatcccgtt 51960
gaaatgacgg gtttattgct cctaaagcat tgctggcgat gcagttgcct tgtaagcatc 52020
tgaactgggt tcgactcctg gtgggagcac caaatttaaa aattcatttg ccttctctct 52080
tatttcttgt tataatattt atataaatta atgagaggga aaccaaatga aacgttatct 52140
ttctgtcgtg ttccaaactg gtggtcaacg ctacacttat gaatttcctg agtcctggaa 52200
aattaaagaa ggtgatcaag ttgttgtatt aacacctcgt gaaggcttca aagtagtaac 52260
ggttaaacaa gtctttccaa aagatcacga accatctaag tctattcagt acaagatgat 52320
tcacggtctg gtacgaaaag tacctagaac agaagtcgaa gtagataaaa ctggggaagc 52380
taaataccag tatacttctt acttggatga aatgatgtaa tgctctgata gttcaacagg 52440
ttagaacagg cgaccgataa tcgtcaaatc ttggttcgat cccaagtcgg agtaccaaat 52500
tatagttagt tggcagagtg gttatgcacc tccttcatac ggagcgacta cagtggttca 52560
actccactac taactaccaa agataagatc aggcctttgt gccagatctc tgtgtttatt 52620
aaactgaatg ggactatcaa tccacaagcc ggactacgag cgattatcgg gtattcactt 52680
aataaacagc ctgcctccca gtttttatct tgcttctggg ttaacaaaaa gctgagccaa 52740
attctaacat cagggagatc taatgaaagc atatcaaagt cttaagtctg gcgtaattaa 52800
cattgttcat aacaatcaga tcattcaact atattctaaa gatggtgaac ttaaacagaa 52860
agttctagtc gaagatctag aaggtatcac acctcacttt gaccctgagg cctttcgcat 52920
tgtagatgtg gaagtggttc ctcaagtcga gggtggtcag cacctaaatg tcaatgtgtt 52980
aagccgtgat cagcttttgg atgcacagaa acatcctgaa aaatatcctc agttgaccat 53040
ccgtgtctct ggctatgctg ttcgatttaa cgcattgacg cgtgaacaac agaacgacgt 53100
tattagtcgt acatttactc aggcgatata atggggtttg gatacgattt ctcccagcta 53160
cgcaaggtag tcgagcaaac tccatttatc tcccaagttt taggagaaat gtgtggatcg 53220
agcattaaga cgtcatcacc gacaacgtgt gaagaacaac cgcaagaagt actggacagt 53280
ctttccacat gaagaaagcc ctaagcggtt aggtattatc actactactc cttgcatctg 53340
ttcttgttgg atgtgtggga accctcgcaa gtactataag aatagtaaag caggcatgaa 53400
aatctcggag attaaaaaga tggatgcaat gattatgggc atcaccttta caggtggtat 53460
ctctgatgaa ttcgacggtt ttgtaactca ggatattgtc gaaattatct aattaataat 53520
aaaccccagc tattgaataa gtagtctggg gttttcgtgt ataaggattc tcgaaaatga 53580
aaatttttag agtaaattac tcaggactgt ggtggttagg tggtacagct attgttgttg 53640
cggaaactgc ggaagaagca atcgaactgg ctagaaatca tagaagttct atagaattcg 53700
agaatgtgac agtagaagaa ctccctagca gtggggtagt atacaacaac aacggagatt 53760
actaatgtta tattttgaag ttcgcccaca ttaagttaat tcaaaagact taatggaggt 53820
aatatgagcc gtacttatcg tagacaatct ggtgatcagt ggtggaagtc taaagaacaa 53880
ttctctcaag aatattcttt catcaaagaa aatggctact ggattcgtgt tatttatgct 53940
aaatcctggg ataaggtaga acaagaaatg gaagaagcaa ccattcgtaa tgagaaaaag 54000
gatggttata actggaattc cattagtaag aatgttaagt ggcattctaa taaaatggtt 54060
cgtcaaggta atcgtcaaga actccaccgc gtgatgaaag atccagaaaa ttacgactat 54120
aatcgcgatc atgacatgcg taagcgtggg ttatggtggt gctacgacta atttctgaaa 54180
taattcattt gctaaacctc tcttttcttg ttataatatt tatataaatt gatgagagag 54240
gtttgaaatg aaaaatcgcc tagactgtgc tgttcacgta cttgagcatg atgttgttgg 54300
gacttgtatg tcctatcatg aagtaggcag aaggattgtt aaacgtgttg ctgatgtttg 54360
tagtttgaaa atcacagaag atgaagcaga cgaactgctt gagctagcaa ttgagaaaac 54420
tgagattttc ttatgcgatt cctgctcttg gtggtgtgaa gcccatgaac attcctacaa 54480
cgattatgat gtatgccgtg actgtaatga ggaagaccat gatgaataag tttattattg 54540
cactgatgat ctccgcagtt agtttcggtt ctctggcatc taccaaagtt tctatgaaaa 54600
atgggaatat tagggtacaa cagaacggca tcattactga atatggtaaa gtcagattag 54660
taaaggagcg taatggtaat atagaaatta tgaccaataa aaacttctct actccagcta 54720
ttaccattca gcgtaacggt aatattacta cccaacgttc caacaattct gactcgttta 54780
cttgtcgtta tgattgtgct gcggaagtag aagaatgatt accttaccac tttgggatgt 54840
aatggaaaag tttggtaaag atttgtgccc aggttgtata ccattgtttg ttactattct 54900
aattgacgaa aaaggatctt aaatgattat ctctccattt gctttgtggt ttgttattgg 54960
tgcagttgta gcgatttata gtctgattga tgatctgtat atctctaaaa ataaagatgt 55020
tattctttat gttataaaca aatatcgtca gccagaatta gcagaatttg acgatgaaaa 55080
gatgttcaaa ttcatcatgt caggtatgct tattatggac accttgttcg ggccagtagc 55140
tacttatttc ttcttccgta aaactcgtaa gatgaaaaaa ttccgtgctg aaatgcagag 55200
caagtaaaga actcgcgtag gaccgagttg cgtcacctgc aactgttacg gataagggag 55260
tcgtgcccct tagtgcggta agtggtgagt tggtcgcgga cctcaacttt aactggagat 55320
atcatgagct ttattttatt cattgtagca gcatggttta ttattggttt aggctatgct 55380
attgcagtta ttcgtcatct ggatgaatat tctgctgaat ggtttattaa gtacctacgt 55440
ttagacgatc aagaatacga gttcgacagt gatgaggaaa ggcaggctgt ccagtcaatg 55500
actcccaaag aatgcttact atacatgcgt gtaattggtt tctttggact gttggtagca 55560
ggaccgctag gtcttactat tgctccttac aaagaagtta ttgacgacat taaactatgg 55620
agaaccgctg gatcgctacg tcgtgctaga gaattaagta aacaagagtg atgataatca 55680
cctcctagca agaataataa tggcaaagcc cagtcgcaaa attgcaggct gggctatttt 55740
ttgtaataaa atttcagttg ctcagatgcc acaagtatta tataatatgt ttatagattg 55800
aggcaaaagg ataatttatg agaatcattt caaagttcaa tgatgtatac gatttgcaga 55860
atagtttatt tgatccagat cgtgtctggg agcgtaaaac tgaagagctg ctagtaaaag 55920
taaccgacga tgtagaaaag aatattgttc ataatcgtca ggtgtttcgt gaaggttcct 55980
tatccttccg tggtgatttc gaatactcag taaatccact gtttattgca ggtgaagttt 56040
actggttaca tgaactgtcc tgctggcatc cagcattcag tttcaaaacg ttcaacatcg 56100
atgctgtgtt cgataaaatg gaagaaatgg gattacatgc ccgttcttac ttactagata 56160
agaatcgtgg tgatgttcgt gaaaccatgc gagatatgtt ggttgaagca aaacctaaag 56220
cagaacgtat cttatctgaa ctgcgcgtac caattgcata tgtgaaaggt attaagaaga 56280
acgataacga tgatgttcgt aacttcgtaa ttcagacaaa cattcgtttc catcagtcag 56340
gtattccgtg gcaggaaatt gaaagtaact tataccgttt acaccaagtt ctcgaacagt 56400
atatcttcgg tgtactagga actggtgagc ctgatatgat taccgtatca gataaagaca 56460
ggttagcagc acatggattt gatacaaaaa cttctttcag gaatatggca aggtaagtgg 56520
tatatcctgg gagctggtct tgcgataggt atcggattcg gtgcttatca tttagtaaat 56580
aaggttgaaa ctttagcggg agatcttgca gttgcgacta aaaagatctc cactctagag 56640
acttctctta ataaagtgaa aactgagagt gaacttcgtg agactcgaat gaaccagtat 56700
ttcacaatga ataatatttc gcaagcagat ctagacaaaa agattaagca gttagataaa 56760
gctcttagtc gtcaagatgt tatcgctgca aagcctggat tggtaacatt aattgctaaa 56820
aagcagaata aagagttcga ggaaagatta gcgtgtttaa ctggaaactt ggagtactgc 56880
tcgcagccgc aatcacaatc acaggctgtg cagaagaaat aaaaccggag ccatcccatg 56940
agttaaagca agctcatgta gattggccta aagtacttca gccgtgtagc tttgatttca 57000
aattcgaaaa gaaattagct acaaatggtg aagatggcgt agtagttgtt gtaccttaca 57060
aagattggaa tgcaaaagca aaatgcgagg aagcagttta ctcttacatt tcacgactga 57120
caggtatggt ttgcttctat cgtcaagatt tacaagaaaa acgttgctta gtttattatc 57180
cacccattac aaataggaaa gattaatgtc agtattagtc ggtttacatg gtgaggctgg 57240
tgcgggaaaa gataccgttg cagaattaat gattgactgg tgtaatgaca cgttcccaac 57300
gtgtttattc cgtcgttata gttttgctaa acccgtttac gaacttgcat ccgtaatcct 57360
tggggtaact ccagagtttc tgggagagcg caggggaaaa gagattgacc aatggtttac 57420
agtcacacaa tctcaattgg agcgagctag agacgtatgg tttaagtatg gtatcgataa 57480
gtttgaggac ttctcgtacg tttggccgat atttgaagga aaataccttg atcctcagca 57540
gttaattggg caagctgatg atgcgctgta tagcgtattt atctccccaa gaaaaatgct 57600
acagcttgta ggtactgagt taggaaggca gctggtgcat gaacgcattt ggctcataat 57660
tctggagcaa tccatcgata aagacgaccc agacgtcgca atcgttaccg atgtgcgttt 57720
ccccaatgag ggagcgctaa tcatggaaac caattctcta gatatggatt ccatgcttat 57780
taaggttgaa tctggtatta ctaataagta tcgcatcgtg acagatcatc cgtctgaaag 57840
cggtattcct gaaaaataca tttctcataa atttgagaat accttcaacg gacttgatgc 57900
acttcgtacc gatgtgtacc acttctgtga cttagagcta gaaccactag ttggataatt 57960
aaggatcact atgactaata agaaagaaga aaagactaac ctgtttcaca ctgttcgcca 58020
atctaacgaa tatacctttt tctttgatga agaattaggc tcaccggatg agtatcgtga 58080
cctatccatg atactgatgc aggctggtga agatgatgaa attaatctga tgattaatgg 58140
gcctggtggt tacgttgata ctgctgcaca actatctaac ctaatcgcta actgtcgtgg 58200
aacggttatt ggacacctgc tcggtcctag tgcttctgct tactgcacaa tcttcctgtc 58260
ttgccatggt tgggttgtac atccacatgc tacgcttatg ggacatacgt tctctggcgg 58320
attctgtgag aaaggtcaag aaatcaagaa agcctatgaa tcctacaacc agtttgtaga 58380
agatatgatg ctagatattt actttccgtt cttctctgtc gacgaaatcg aggatatggt 58440
aaaagagaat aagaatatct atctcaacag caaagaaatc aataagcgta ttgaaatttt 58500
ggcgaattat cgtaaagagc agtgtagtaa agctcaagca ccgcacccag aagaacatgg 58560
agaagtgtaa gtagttttaa gccagagttt aacgactctg gcttttcttt tgcctaaaat 58620
tcttcttgac aacgacaaaa aattgtgctc cagaagtgat cttaaaaaac acattgtaat 58680
tttactctag aattagtata atggtagtgg ttagaggagg ttacaagtgg aaaagtttat 58740
acaactatta acgatactgc tacaagaagc gaaagatcca gcttcactac taaaacgtct 58800
gctaactatc ttagttgctg tcattatttt cttgtttgta agtaacacta gcgaggtgat 58860
gtcattttta aagactttct ccacgtcttc ggttttacag gatgttcaaa cccaaaggat 58920
agataacttt cctaatgtag cacgagaaaa gagtatggtt cttttctccc agactggtgc 58980
ggatgccgtt tttgtagtaa agtataaacc agatgctata aatgattatt caaacattat 59040
tgcatgggaa agcaatgcgc aattggatag ggctgacttg gccgataaag cagtaaataa 59100
gacgtctgaa ctttatagac gtcacctgga aggctttaac tacgctgcgg atttaagtgt 59160
aaaagtaaat aaatatacgg ggtcaaatat accagcgttt aagaacatta cttttaatta 59220
cgtctacact tgtccatatt tcaacctaaa taatatctat gctggataca taggtattgc 59280
atggaaggat aaacccgtag atacagctga ttcggaacaa tttaacgagt acttagcaaa 59340
gctctgttcc ccacaacgga gatcattagg tagatctata tgagttttaa atttggtaaa 59400
aatagcgaaa aacaattagc aactgttaag cccgagctac aaaaggtagc tcgtagggct 59460
ttagaactat ccccatacga tttcacaatc gtacaaggta ttcgtacagt agcacaaagt 59520
gcccaaaata ttgctaatgg tacttcattc ttgaaagacc ctagcaaaag taagcatgta 59580
actggagacg ctatagattt tgcaccatat attaatggta agattgattg gaaagatctg 59640
gaagcatttt gggctgttaa gaaggctttt gaacaagccg gtaaggaact tggtatcaaa 59700
cttcgttttg gtgccgactg gaataattct ggtgattatc atgatgagat cgatcgtggt 59760
acttacgacg gtggtcacgt agaattagtc tgataataaa tcaaggcggg gtaattcccg 59820
ccttttaaag caaagggggc tttaaaaact taatgatagg agaaagccat gtttgcagaa 59880
ctattcacca tgatattact aggagtatgg aaaataagtc tagtagtttt catcttaatg 59940
ataattctca ctgttgtcgc actagttacc caaaatgata cattaaagaa agtcgttcat 60000
gggctagagt atataattat gggcacattc ggcgtttgca aatgcaactg ccagagaaat 60060
acgaaatatt gttggctttg gatggagtta gagaactgca tcacagtagc cctggctgtc 60120
tcattcggta tgattcttat ggcccttact ctagcattga tacccctaat gttagctggg 60180
ggagtcacgg cttatttcac ccttttctcc cctatactta tgtattcaat atatccaata 60240
actatgtact tagttaggaa gagattctca catcaagcac actaatataa aaaagtagtt 60300
gactttccgc tgaaattttt atataatatt tgtataaatt gaatcagagg agtttcacaa 60360
atgtcagatc gttactatac tcagatggct aaccattaca atatgactcc gtatgaattg 60420
aacatcgcat tacgtgacct cgattcacca gagcgtgcaa aacttgagaa aaaggtggga 60480
attcgtatgt ctagcaaggg caagaaactt actcgtattg acttaaatac catgctaatg 60540
gaagagcttg gcgtgaatat tgaaggtcag aagttgcctc tcaatgttct tgaaacgatg 60600
ctggataaag tcaaaaagaa aacctataag aaagtacaag taccggaagg caggctaaaa 60660
gccccttatc aggctgctgt atctgagtgc ttaggtgcta ccgcagatct tagcactgca 60720
actgttaaag tgatgcgtgc gtttttagag gcagttaata aattgtgatt acccaagaaa 60780
gattgaagga gcttctagag tacaatccag atacagggat atttatatgg aagagccgaa 60840
agatagatag aagtggtttt aacaagcaat tcgcaggaaa agttgctggc aatcctcaaa 60900
ataacggtta tatcaatatt actgttgatt ccgaagttca tggggcacat agattagctt 60960
ttctttacat ggaaggctat atgcctgcaa gggtagacca cgaaaacatg gttaggtctg 61020
ataacaaatg gcttaaccta cgagaagcta cagtctctca aaatggtgct aatgcgttaa 61080
agtattcgaa caatagttct ggatataaga acgtatactg gacaaagaaa acgtgtaaat 61140
ggcaagtaca ggttatgaaa gatggtaaat cctactcggg aggatacttc acaaacctca 61200
aagatgctgt agaaactgcc aatgagttaa gatttgaatt atttggggaa tttgccctat 61260
atgaaccatt taaaggagat aaacatgagt aaattagtat atctattgaa gggatctacc 61320
tgcaatccgt gcaaaatgtt cgagcctgta tttgataaag tagttcagga ctacagtcta 61380
gaagttcata aagagactga taacacagaa cttatgcaga aatttggcgt tcgtcaggtt 61440
cctgtagtag ttttagcaga tcgtctcccg aatggtcgtg tggaagccaa ccacattctg 61500
attggtcgtc aacttcgtaa agaaactatg catgacgcaa tcaaaaactt cctggaaaat 61560
aatccagaag attaataaat ctaacccaga tcttagctga tctgggtttt ttattacttg 61620
ctttctgcta aaaattttga tataatatat gtataaattg atgagaggag tatcacattg 61680
aattacgcat atcttataat acataacaac gtggtctgtg gtactcgtgc tgtagaaatg 61740
ggaatcacag aagaaaaata taagtcactg cctgaaaagg agcaagaata ttatgttgag 61800
caagctgctt gggaatatgc tgaggcttat cccgaagaac gtgaaggccg tgttactatt 61860
attgttactt tggggctggt gggctgtgat accgaagttg atacagatat agaaacactt 61920
gaagaatggg aagagttgga tattgcagaa caaaatgcta tcatccatca atctttctgg 61980
gaagcagtag actgtcacgt tgtctttgag ccaaacgata cagaagctga aaaacacact 62040
aattggatga ctcgataatg aaaaaagaat ttaacctgca cgaaatgtta gtagtaccag 62100
acgatgttaa cctgttcttc gtaggagata ttcacggctg taatgatatg ctggaagatg 62160
ctcttaaact agcaggatat aaagaaaagc gtgactatgt tgtctgtgtc ggcgacttaa 62220
ttgaccgtgg cccgcagaac ttacaggttc tagccaaatt cttatataac ccacgcttcc 62280
gcagcgttcg tggcaaccat gatcagttta tgattgctgg tgactacgcc aactggatgt 62340
ataatggggg aatgtgggca atgaatgatc tggataccga tacaattaaa ggtatcgcag 62400
aagatatggc agttaaaatg ccagtattca tgactgtact ccatcgtggc aagaaatacg 62460
gtgttgttca tggtggaata ccattccaat ataaagatgc tggattcgat gtacatacac 62520
ctaattggga cgatattatt gatcatatcg ctgcaagtga aaatgatcct aatgaccatc 62580
ctagttatta tattgaacca tatttgtggg atcgtgatgt aattcaggaa attggttttc 62640
atctatctaa gcaaggtgca gaacatcctt atttccagcg ttatacaggc tttaaagatg 62700
aattgacggt ggaagtacca gaagttgaaa gtgtggattt cgtattccac ggccacactg 62760
gtgttcctta cccgattcta tataagaacc gtgtttatct tgatactggc ggtgttttca 62820
acgggcagtt gacggttgcg caagttaatg atgaagccgg taaaatcatg acatttacca 62880
cagataaaaa tgatagctgt ggtgtgcaga gggttttata atgaaagttt atttttggag 62940
tgatctccat ttagggcatg ttaatattcc taagtttcga actatgttct ctagtatgga 63000
agagcatgac gaattcatca tggatactat tacatctatg aaggataagc gtactgtatt 63060
tttcatttta ggggacgcat ttgtatctag aaatggtctg gagaagtttg aaaaactatt 63120
tagcaatgtt aggactactt tagttctggg aaaccacgat ttggaacgag aaggcttatc 63180
ttttacagac ttagtaggtg tagtagataa tgtgcagtct ttagttaaat ataagaactt 63240
ctggctttct catgccccag tgcacccagg agagttaagg ggtaaaaaga atttacatgg 63300
gcatactcac tttgaattaa tgggagatcc acgttacatt aacgtttgtg ttgagtacgc 63360
aaaatctcca gtaagattgg aagatattat gagtggcaaa tatacctccc atgataaaac 63420
tagttggatg ttggaatgat aacgcaggaa aaactgaaag aactcgtaga atattttcca 63480
gagactggag agtttatatg gaaagtcagt aaaggcccca ataaagcagg tagtaaagca 63540
ggcactttat ctggagatta tattcgtata gtcatagagg gaaataataa gttagcccac 63600
caatgggctt tcttatttat gaaagggtat atacccgaat taatcgacca taagaataga 63660
ttaggcattg ataatgcctg ggaaaacctt agagagtcta ctaaccagaa aaatctacaa 63720
aatcaaaaga aaaagaaaag caagtctgga tattctggag tatcctggaa caaaaaagca 63780
cagaaatggt acgtacaagt taccaatgct gatggaataa gaaaatctgg tggttacttc 63840
ccttatatag acctaagctt agccgttgat aaagctaata gtatgagagc tttataccat 63900
ggagagtacg ctatagtaga aaactttagt agatatatac caccaataga cgaactaaat 63960
aaataattta cttgctaaaa tgcctaagtt tctgtataat tactttataa attgatgaga 64020
gaggtttcca acacaatgaa tactaaaatt gataaagcac tcttaatcgc aacagctgat 64080
aagtttgagc aagttaaagc tacttttaag gcagtctttc agtcctatgt tcaagacaaa 64140
tcgaacccta tttctgagcg tttgatgatg tgggaatgtt ttgcttgcaa tgctctgcta 64200
atttccgatt ataagggaga agtatctgag gaattgcatg agatctttat taatgaggat 64260
actccacgct atcagttagt gaacttccaa gatctagctg agcaagttat tcctgatgat 64320
ctttgggaca agtattacgg tgatcctgaa gaagatggta tggaacctga gaagtgcatc 64380
gaactgatct gtaaggatca tcctgaaatt gctgagaaat ttgagttagt atttgcttct 64440
gagttctcag gtttcgttaa cgattggtaa atgataacac aagaactact aaaagaacta 64500
tttgaatata atcccgatac aggagacttt ataaggaaag tcagtaaggg tagagctaaa 64560
gctggagcta tcgctggcag tcccgataaa gatggttata tatttatcgg gataaacaga 64620
aaaagatatg cagcccatag attagctttc ctgtatatgg agggttatat cccagattta 64680
gttcatcact taaataatat aagaaaagat aacagatggt gtaatctaaa ggatgccaca 64740
tctcaggaaa ataatcgtaa taaagtcgca caatctaatt ctggatatct aggggttagc 64800
tggaagaaag atcgccagca atggagagtc caagccagaa attctgaaag tactccagta 64860
catggtggct actttagata ccaagatcta aatttagctg ttgaagcagc taatatacta 64920
agggaagagc ttcacggaga gctagcagtt aaggaagtct ttcgcgggtt gcttccagca 64980
ttagaagaat taaataaata atttacttgc taaatgtcca gaaatcttat ataatatatt 65040
tataaattaa tgagagagga agttcaaatg gaaaacatga ctaaaactga aatggcaaac 65100
gtattggcta tcctgttgga catgcaaggg tttgaaggtc agctcatgaa gatgtctatt 65160
ccggcaatga aaaagatgta cgattctctg aacaagaatg ctatggcatt caacctcgca 65220
aaacaggaag cacgttttgc gaaagagcat caagcaactg ccgaacgtcg tgcagcatct 65280
tttgagcgtg aagttaagca actaaaaggt aagaaataat gactaacgga aaattcttac 65340
ttgtactagg gctgaatgcc ctagtttccc tggctatcac gcttggtgtt accaatttca 65400
cgctcaacca gcgtgagaag atggaggact tacctttatc cttctcagcg gttgaatata 65460
ctaacccaga agcagatgtt atacgttacc aagctaacct agacgagggt ctttcgaagt 65520
attctggtgt ggaagttttt gatgcgtata aactgaatga tatagctgaa gctaagcttc 65580
agattgaaga aggtaaacgc ttctgtaaag caagtaactc atccctagta aaaattaagt 65640
tttataaaac tataagccca tatattgaat gtggcaacgg agtatttaaa tgattacaga 65700
aattttaatt ggattgctgg ttctcacgac tttagcagca attggcggaa tcattggcat 65760
tattaatgct aataaaaata tggataggat gcgcctcacc aatgatgggc tagttaaacg 65820
tctcgttgaa caggaagaag atatcgaatt agcacaaaga cgctcagatc ttcttaaaga 65880
aaagctaacg aatattgaaa ctcttgttgg taacactaaa cttcctatta aagtcctgcg 65940
cgctcaggta atcacggaga ttaagaaatg atgttaatta tcttagcttt ttatctggtt 66000
attgttggtg ttctggttac taagtatcat acctggaccc cgaagaacgt tggtaaagta 66060
gcactgttta ttctgccagt tcctatcatc atcgttgcaa tgctgttcac aatgttggcc 66120
ggtaaagtga caaaaactga cgttaaacgc attgtagacg aactacagca atcttctgac 66180
atgatcgagg atattctgaa agatgaagct taattttagt gaattaaccc tgctggacga 66240
gtttctaggt tttgttactg aacgtgggtt tattaatgta gatcctcaga cactagaagt 66300
cttgcaatcc atccagaata aaactaaaac agatctggaa gacttatggc gaccactttc 66360
tgagctaact cctcttaata tgagagtgat tgttaaaaat attgaaactg gtgaagaacg 66420
tgagatggtt cgtaaagaac tggctaatag ttattctcca gagtccctag tgatgcatca 66480
tgacgatgaa ccagaaacac tttggacagc ccattatatt tggcgtctgc cttgattgat 66540
ttaacaactg ttacggcaat cctaatcttc ggtttagtgg ttgccgtttt cataatcgtt 66600
gagcaggcca aagtaatacg gagacttaaa aatagatgat taatttttat aaccctaact 66660
tgcagaaagc aacgtgggat tactttataa ttctaccagc ttttatgtta agttgtgcaa 66720
tcattaacat tatgtatcaa gaagctggtt ggacagtcca agatttccac atttcacaag 66780
ttcttccttt catgtatgaa ggcgttatcg cagcaataaa tggggttcgt aaatgacagc 66840
actgcaaaga ttacagaatt actggtttga tggccaattt aatgatttat tcatccagtt 66900
gtttgtcgaa acgaacggac gttttaatta ccgcttcttt aatactttcc atgaaacgaa 66960
gttctcgcat acagagatta acgcagcgat tcaagatctc accggatcta aggtaattcc 67020
ttatcgtgat gtagattttg cacctgactg ttttggtttt gagctgttca agaaagcgta 67080
taagttcggt aagtttgaaa atgcacgaca ttgggttcat gatttctggt ataataccga 67140
tatcgttcct agccgtgtac tgatcctcaa ctggattgct aaacaacatc cgccgaaggc 67200
acaatcggca ttccttccaa ctgatacagg aaatctttac catgacacaa aagaaaaatc 67260
cagtgctaga gcagatgaag cagttggaga agcagattga ggaaggaagc gttgatggac 67320
agtcaattgt caatcatgct tttgatctta cagtaggatg tgctaatccg ctggtggcag 67380
gtgaaacagc agatattctt gggcttttaa tggcagttgg ccagttaatc gagattaccc 67440
aagaagagct agacaatgac gaaaaagtaa ctgttcatga tgttcttggt gctctagatc 67500
tgattgttaa tgcttatgcc tataaacgcc gccactcaat tgatgaatat ctagtttctc 67560
gtgagattac cgaatctctt ctggaaactg ctgatatggc cttaaacggc aagcaacttc 67620
actaatccta gaggccagga cttaattgtt ctggcctttc gtgtatacta tgagagataa 67680
aataaacaaa ttactttatg aggaagcatt acaattcccc ataaatagat tcactaaatc 67740
agatggatct attaatagaa ctaaacttaa acaacttcat ccagacttcc agcaagacgc 67800
acttaatcta atctttatca ggagagctgt agaagcacat ggtaagttct ttggatacga 67860
gagagttaag tataaaacta tgcaacagca agttgagatc tattgtccag atcacgatgg 67920
ttacttctta caaactgcac ggtctcactt agaaggacac ggttgccgat tatgtgcaca 67980
taaagttgtg acacgcttaa cagaatatgg agtgtacacc gtaccagcac ctttccacaa 68040
atttgtagtt gacggtgagc acataatttg gtataataaa gatcaaaagt taaaacagga 68100
gttaaagaaa gatggagtac gtagtctggt caaaccccct agctctactc tcccacaaac 68160
tgttcaagac gacgtgggga aagaatgaga taattgttaa cgagagtcct cttaatcatc 68220
aacatccttt agtcccagct ctctacaaga ttattataaa tggtacagaa tacctagtag 68280
atgatttcgg taaagagttt gtacaagaag ccatagacaa caataacatt tctataggcg 68340
aattaattaa tagagcatct aactttgata agagttggat gttaatttgg agtaactaat 68400
ggaatatgta ttaattatct tagcaattgc attagtattt gctattaaca ctgctgtaca 68460
taagactcgt gaagccaatc aggttattga cgataacgtg aagttgcgta gcaaagttac 68520
taatctcaaa caacagcgtg aagctctgaa gttattcgtg gctcaaggta gcctagagca 68580
tgcaccggaa gacttcatcg tatatcttaa acgttacata ggaatcaaat aatggaaaca 68640
ttacttcttg tactactagg cggttcactc ttaaccattc taggcttgat aattatatgt 68700
agtcgcctca atgttaagaa tctagaatta aatagtgaga acgagattct taacagagaa 68760
attaaacaac acaatatcgc agctcgtaag ctgttagata aactggagaa taaataatgt 68820
ttacacaaca aaccattaaa gtagttgcag atcatgaact cgacacatgg gattacagct 68880
ttaaagatct gactgctaca gtacagttta aatctatgca actgtcgttt ttgcacattg 68940
atcttcaggc ttgcaaagag tttcaggcta aactcatgaa tgctcgtgct atctttggca 69000
cggctcgcat cccggctgat aatctgatta atgcactgat cgatgcagga tacaaactcg 69060
tgaagactga agtgaaccgc ccgagtactc cagtgactat tcgagatccg tggcagtcgg 69120
gaccaatcgc aatgctgaat aattccgata aaagattaat gtcggatatg cacaatgttc 69180
cttgtggtgg cgtggtaagc aatacgcaat catataaatt tggggaagca tctagcgtag 69240
acgagtggaa taaagctggc gttgtaatga tgggctttgc aaaagatcgc ggagcacaag 69300
aagactccgg agtttctagg aactcttata ctatccattt gagcaatcct agcaaagatg 69360
gtgagcttga agaaatgtta acgaaagtat tcgaagctat tgatgcagat aaggctagag 69420
gtcgttaatg gatacactgc tctcttacga acatgaagac tttagtataa aacttattaa 69480
tacagatacg tttggtattt atttaaaaga agaaatcaga catgttttct ttgctaacca 69540
acaagatcta cgtactatta ttgagttaat aagtaaaata ccaaaagaag cgatcttgca 69600
tcactgttat ctagcagatc ttatcaaaca gttaggttta gtatgcgtag atgtaatacc 69660
acgtaataaa aagtaactaa tacacaagcc tctacggagg ctttaaaata aaaagcaggt 69720
catctgacct taaaaaggaa acacaatgag tacatataat tttaaagaca aactgagaca 69780
aaatgctggc aaaggaaaag ctaaggaaac aaataatatt ccaaatgcta agatcctacc 69840
gttccctgaa ctaacccaag aagaaaaaga taagctagca tttatgtttg atactttgct 69900
aggactgaat ggacgtttat actctacgct ctcgttaaat gagaaacgtt tagtagagtt 69960
ctttgggaaa cgttatcaag ttacatctaa gggtaacgct cttaaagaag gagaaaaacc 70020
ttacacagtt attaagatta gtgcatatgc acatcgtttc ttaattcgtg cgctagagac 70080
agaatatttt acgctacctt catttagtgc attccacatc tttgaagatg aacgaatgaa 70140
tattgatgat ttatatggag atcgttatca atattctgga agcccgttcc atgatgcctt 70200
aagaagctat ccaaattgtt atcttccaat tagagcatca caaggtaaag caagtgtctt 70260
tgcagtatac tcagggtcta aagcatcgga tctagaaact tatatttata aagaacgtag 70320
atctgctatt ccagaagaag cacgttatga aaatccaatt ctaccaacta aagaagtcaa 70380
ggaaaccaaa acttctacta ctcaaacaga atctccttat ctgaagtttg agaaacaagt 70440
tggatgggtt ctagaaatgg gtatagcttc accgcttgct gcagcattta tcaataggta 70500
tctagaaatt cctgacgagt tccacacacg ttataataca aatattcaga tcacagatga 70560
attaatagct attgcaaaag atacacctac taacactgag gcgtatcaaa aagcatacca 70620
agagttactg taagatcctg catgtctcgg tgcacaatac ctgtaccgag actcaaatac 70680
aaactctcgt tgacagataa ctaaatgtat gttatcataa ctctatgagt taatagaatt 70740
aaccatttag agttgccaca cagaaggtgt ggtggttcag gaatcttaaa ttactgactt 70800
gtagcgtagg ttacggagcg acaggacaag tcaacaggta atttaataaa gattactaga 70860
aacatcacga gaccttctgg cgggggcaaa gctctatatg agaaccctgg gggatcttat 70920
acttatataa aaactaatac tgttgaatag ataaattaac gttcaagatg atacaccggt 70980
tgccgctcat ctgctaggac ggcacaacac tttctagtag actcctaagt ttctaggaat 71040
agttaagcac taactctaga gtttacacta aactttaaat ctgtttctca cgcattctag 71100
ggcagttttc atattcctag aaactctagc aataggcggt ttaatacagg gcggttatgg 71160
ggagggcggt tatttctagg aaaaataatt gactcttgtc agtctggaaa ttgctatcgg 71220
atttgcacat tatgtttcta gaaatttatg gtcggatttg cacattgata ggagtctcca 71280
aagtttttag gaacactgaa aatagcagca ttatttcggg ccgaaggccc atgattgcct 71340
gagtttttag agccacagga attttcactt gtcaagcgat tttgccccat tttgctaata 71400
aaatttcgct ttatctccac taatctcaat tctagccaaa acatcccaca ttatccccaa 71460
aaatgcagca aaccggtgct aaagcaccat caccgctgct ccttccgtag ttcctcccaa 71520
aatctcatcg gattcgcact tctccccaaa cccacacaat cacacaccct ccaatcgaag 71580
tcacaaaaat cttgtcggat tcgcacacct taacacttgt cggatttgca catactctag 71640
taaatccagc tggattgcat cgcagttgcg gattgctgga gtttttagga aaattcatcg 71700
tcggattcgc acatataaaa aatatttgcc atcggatttg cacactacca aaaattgtcg 71760
gattcgcaca tacacgcccg cacgtaacac ccgtggagca atgtcggatt tgcacaaaag 71820
cgcagggcaa aatgaaaatg ccctgctggc cgaaatgcga atgataatga gaacgattcg 71880
catttgagaa gttgaatgcg aatgagaatg atttgcattt ggaaagcgga atgagaaggg 71940
ttatcattta cagaattgaa tgcgaatgag tcgcataatg agaatgatta gcgtttagaa 72000
atggaagtga gaaacactgt tatttaagtt atccacaggt ttattaacag cacgatttga 72060
cttgacaaaa tcctagcgat tttgaggcgt tttcaacaga cttatcaaca ggttattcta 72120
ctgtataaat atacagtata ttccagcagg agaataacct aatataggtc taaaacgctc 72180
tcaaacgcat tctaacgcgt ttatttttgg aggaggtatt ttaataaggg gaaatttgag 72240
gaagggcaga aaaggcgggg ccgatagttg cgccccgtta ttattagcgg tagatgtatt 72300
gttgccagta gcggcggata ttgtcgccac cgtcgggatc attagcagac ctaatattac 72360
ctataaaaat atatagtttc ataacgccat cgctcctaga atgagaatga ttattattaa 72420
cattgtgaag ccattaccaa atcgtaaacg ctgggaatgt ttcccagcgg tattattaga 72480
tttatgggat tgcgttttac gccaccagct attcataata aagccgccag ccatcattaa 72540
acatttcacg gctataaaat tcatgattaa atttatagcc atcctcagtg gtgatcactt 72600
catatttcat gaataaatat tcatggctag aaaaataacg atgggttact tttttgcctt 72660
ctagcatagc ctttacagct tcatttctag tcattgcgcc acctttcgaa acgccgtttc 72720
aatactttca gcggttgatt gaatatatgc gcctttcata atcaggcgct ccgtacatac 72780
tttgcggaaa atgtcaataa attcattttc attttccgct attaatgctt ctcccataac 72840
ctgaacgtca caagacccaa aagttaaaaa ttgaaacgct agttcacggg cgacaacggc 72900
agaaatttta taatgtgctt tgccctcata gatattaacc acgcctttat aaactggtga 72960
aagctgattt aaatagtttg ctaataatgc cgaacggcaa ttagtaataa agcgattacc 73020
ctcataaata accattaaat gatcctcgcg tcacggataa ccataatcac tagcgtgatc 73080
aacattgcag cactagacac gccgcaaaca aaagttaaca ttttaacaat tgccccttcc 73140
attataatac cccacgaaat gcagcgtaaa aagtggcaaa gtgtttaacc gtttcggcta 73200
ccaaattgcc gcgcttgtca aatttctttt ctgtaacacg aaacgcgttt ttgttgcgtg 73260
ctacatattc gattttacag tctggacgct cgaacgtgtt aacatccacc agcttaaaac 73320
ctttagaacg ggcaaggctt acatttttta acatttttct tctcctttat tcatgcagcc 73380
cagcaaataa gaaaatacag cgatacccgc gccaataata accacgtgaa cgccgaaagc 73440
tatggcaaaa agtgataaaa tagcgcccaa gatacccgct agaattgatc taaaaataac 73500
catttgcata actcctagtt agggaataag gggcgaaagt aacgccccgt tattaattac 73560
attaaaacaa tttttgccgc ttgctttttg gtgcagtggt tagctttcaa ttcatccagc 73620
aaaaatccgg ctgtttgctg atcaatttta tcattgtcgt aaagttcctg aatggtgata 73680
taagttttac caatcccaaa ttcagaaacc ataacgctga taatagcggg aacggatacc 73740
cattccatac cattaaaaat tgcagaagat gatgaatgta ccattttaac gcctcctaga 73800
aatcagggaa aatttttgat ccaccgtttt ggtgtaaagg cattataaca aaaaaggccg 73860
ccataatagg cgacctttac aaaactttac attagactat ttctaatgct tccacaccga 73920
aacagcgttt tagttctttc tgtaaaacgg catattcatc ccaacgggaa ttatcaggaa 73980
tataaacaag ctgttttaat cgatttgcat ataataacgc cttgttacca attatttccg 74040
cgccaccgtg tttaataatt agtttcataa cggtattaat aggatcgccg ccatttacat 74100
aaataccgga tttactcaat tgataatgat aaatactcgc ataaacatta tcattttcaa 74160
tccattcccc tgtttcttca tcttcttccc catcactacc gaaacgagaa gcattatcat 74220
atgcgtgttc gtttgcgaaa gcgtccagat cacagccagc gggaacaaaa tgttcctccg 74280
tccagccttc cccgcaatag ccagtatgac aatcaactaa aactatcata tcgtgtttag 74340
cttcattcac actttctagt gaaaagccat atttttcttc tggcataact agcctcctat 74400
taattagcag ggaaataaag agaatttgct acactgtaaa atcctttatt taagaaaatt 74460
acgtcatcac ggactatttc atccattcca taatccagca aagtttcgtc tacaaaaata 74520
catagccagc cataaccacg atcaaagaaa gcatgataat tattcgcgct ttctacatgt 74580
tcccataaaa agggacggcc ttgtttttcc catataggaa tattgctttg atcgtccgaa 74640
taattaacat gtgggctatt cggcattttt tcggctaatt gcgtcaacat gatttgattc 74700
agttgcataa taagtagtct cctaacaatt aacagggaaa tagtaacgcg gggaaataat 74760
ccccgctagt tattactact cactttcggt attagtggta aaagtagaaa tgtcaatacc 74820
ttttaccatt tcatcgatca tttccgcaat attgaaactc tgtaccattt ccagcattgc 74880
cgcttccatt gccgcgcctg ctaccgcttt cgctttctga ttagtcgggg cgtattggtc 74940
aacagctaca ctcagcaggt tggcaatcac tactcgacca atcggcgtat caatataacc 75000
tttaatcata aatggcgctt ttttcgccgc tactttcgtg atttgagtta atgcaatttt 75060
acccgcttcc agttttgcag cgtttacaac ggcggattta ttagcagcaa cgatagaaga 75120
tactttagtc atgatatttc ctttattttc agtagtttgc gcatttgcgc ttgaagtttg 75180
agagtttaca gcctgttttg ctacattgtc aagtggtaaa agtgtaaatc cagcgtttgc 75240
aacgtttgat tcattgccat aacacaaata ttcgtcagga atcaacatgt taccgctagc 75300
gcgttcaaaa cacaatacgc cagttgtttc gattgcgtca ctatcagcac gtttaatggc 75360
gcttgtcaat tcgccccaaa tactagatag taaatcttct acatcgctgg aattaatacc 75420
atttacgcga gtagcgaaaa ccatttcttt ttcttgtaca ttgtaaccac gggcaatata 75480
gcttttttcg tcgtgaccta caatgataaa atcaatatcg cttccccgct gatttcgcat 75540
gatgtgatag tcacggttgt aaagcactag aacgcgggta gggtctagta attgtgtgaa 75600
atcacttaaa caataatgag tatcattatt atctaaaatt tcatcgtgaa tcttttcaac 75660
cattgccgcc gtatgttgta ggcttgctgg acttgatccg tctagcaggt tgcgcaaact 75720
acccttaata atagtggcgt ttgctggtac tgcaattctt acagtagaca tttctttgtc 75780
tccctcgtta atcggtgagt gaatgatata gatatttgtt cgctaggggc aagaaaaaag 75840
aaaaggtttt ttattccttg cctactgcct gtttttatga taaccgcgcg ttttctattt 75900
ctagttctct gtaagcgtac acaaggcttt attataataa gggtagcagt gagaaaacgc 75960
cgtatagcgc gttctaggcc gcttaaattt gattgcggtt gttactgcta ccattctacc 76020
ctgtatattt atacagtagg ataacctgta tataagtctg tgggtaagtc gccaaaacgt 76080
cgcctattgc gtcaagttat tttgtgctgt ggataacttt gtgaataacc taaataatag 76140
tggttctcac ttagccgata gtttcttata gttattattt cttattaatt tatatgtata 76200
ttatataata tgaaatagtt agttggctaa tgagattttc catgattatg agtctgctaa 76260
ctatttcggc tgttacaacc taccagcgtg attatcatcc tagcgattat tagtctgcta 76320
acgatctgat acacgtctaa acttatttgt caatgaaaaa taatagcaga aaatactttt 76380
ttgaaaaatc aagtggataa ctcattaaat ttcatttttg ctttttgtat aagttattaa 76440
cagggtctaa aacgctctgt agcgcattct aagcgcctca aaggaaaaga taggcaatca 76500
taaggggcac accgagaaaa cctcactacg aggattttgg tttttgtcaa gtagggcaaa 76560
gcaaatataa atgaaaaaat tacttgctaa aatggttgtt ttgatgttat tcgcgcccgt 76620
ttcatttaat tttaggcagt ctctggacgc ggtgcggtgg cgtttgtctt tacgtttctt 76680
tacaaaaata aagttgcgcc cttctcggta ttctcctatt atttatcacg tagggcggca 76740
atgacgaact acacggcggg gataacagcg attgacagtc tgtttgacct ttcaaaaccc 76800
tgccatgctc tttaaaaatt agggaacatc ttaaagccta tcggcggtaa acttatacat 76860
tataaggaat cgcagatatg atcaaatcaa aaaacgcttt agtgattagt gcaaaacctg 76920
ctagcgcatt agatagcaga acaaaaaatt tgttgacagt agcgcaacga tctgtaaaat 76980
acagattcaa gcaatacaag aaaggtaagc agcaaggtct ggaaatggtg tggcgtaata 77040
taatgattga tttatataac tcacatcaaa tcgaaaaaag tttaaaaaac gcttgacacg 77100
ctagccgata ggttttaaga tgttccccgt agtaactgct ctttaaaaat ttggataacg 77160
gtaatgttta ggctaactcc tagcggatta taatctaata gcgtttcaaa gtatcgaggc 77220
gctattgtgg aataatcccc aataaaccgg agtattaaaa atgcaaaacg ttattaccgc 77280
gccgaaagta ggtcaatctg ttttcattcc ttttgtaact aaaactgatg aattaaccgg 77340
aaaagccgaa cgtatcaaag gtgcggcgct tgatcccttt gataagattg aagcggtcta 77400
tgcggaaacg gaacgcagta acaacggcaa gcctatttat aatgttcgcg ttaaatctgg 77460
cgacgttgta aagatcattc agaaagatga taaatggcaa gctatagcat aaattagtgt 77520
tacttttata ttcccctgaa atatggggaa tatatggatt aacattagtt aatcataatc 77580
ccttaattaa taattgaatg gagtattaaa ttatgtctaa cgttaaaacc gaaaaaaccg 77640
caaaattctc ttggaacgaa gaaaacaccg aaaaggctgt ttctatgtat tctgaaatgg 77700
tcgctaaaag cgggattgag tttgcgaata gtgacggctt aaaagaaatt gccgccgccg 77760
ttggcgctgc aagtcctgtt tctgtacgtt ctaaactgac aagcgcaaaa gcctaccaga 77820
aaagcgataa accgcgtaaa gttggcggcg ggtctagtgt ccgtaaagct cactatgtgc 77880
gcgtcatcgc taaacacgcc attgattcag gtatcgtaaa agatgccgac gatctggcaa 77940
gccttgaaag tgctaaactt gaaactctgg acgctgtagc gcaattgtta ggcgtagcgg 78000
aggaagtaaa acaggccgca ggtgaataaa ttttaattag cggggaatat tccccgctat 78060
cccttaaagg agaataatct tgatctttta tcctagcgaa gcgtttattt taggtttatt 78120
tattattgcc gccgctttta ttctgaatta tacgcggtca attctagcaa taatcggaat 78180
taataacgtt tacgccgcaa ataatttccg caaaaacgcg aacattcaaa aatatctgtc 78240
aattatcttt cttacaattg gcattgtatt tggcttttgt tctagtttaa tgccgcttaa 78300
ttaaatgttg caaattataa cgccttttaa attgtcctca cttcaggcaa ggcgttatat 78360
ttggcaatat tgccagcttt aatctttatc cctttatagg agttgaatta gttatgaata 78420
tttccgcaga aaaacaagcc cagatcgtta aacttgccgc tgattttaat ttctacggta 78480
aacgtttacg cgctactaag ctggaagttt gcgacgatat atcaaaagct gtttacgata 78540
caacgaaaca ttcaaccgca atttgcgact ggttggaagc aaataagcca gcaaaaccga 78600
aagcggcaaa agctgaaaag gcgattaaaa atgatgatcg ccccgaagcg gcgggaattg 78660
tttctagtac cgtggaacag tgggaagtta aatccggtcg ccgttttatt ctaacgtcga 78720
ttcaaaataa tactttcccg cataaaaact ttttagcaag tctggaaaaa ttcgctgaat 78780
atatcggcgg tgaattgctg gttagtaaat tcatttacaa taaaaacggc ttccagaatg 78840
gagaaggtaa cgaaggcatt aaatacgatc ccgcatttaa taaatatatt tgcgagaaaa 78900
acgtattttt aaataatcgc cgttttgctt tcatggctga aattaacgtg ttaccaacgg 78960
cagactatcc gctttcagga ttcgcagaaa ccgccacggc tttaaatatt gaaggtctgg 79020
caattggcca cgctaaaatt accgctgaaa gtgtgccagc gttaaaaggt gaagtagtgc 79080
gccgtatgta ttcaaccgga acggctacgc ttaaaaacta cattcagcag aaagcaggtc 79140
aaaaagccga ggcgctgcat aatttcggcg ctttgctggt ggagtttgac gacgacggcg 79200
aattttttgt gcgccagctt gaaacaatgg acgaaagcgg aatgttttat gatttgaata 79260
tttgcgctac tcctgccgga tgttatgaaa cgtctggaca cgttttaggc ttgcaatatg 79320
gtgatattca cgccgaaaaa ttagatgatg aatgcgccgt tgcgtcatgg gctagtgaaa 79380
acagcttgct tgatattctc aagcctaaat atcagtttgt ccatgacgtg cacgatttta 79440
catcccgcaa tcatcataac cgcgctagtg gtgtattcct tgctaaacaa tacgccgccg 79500
gacgtgacaa agttctagat gatcttatcg ataccggtcg cgtgttggaa tctatggagc 79560
gtgatttctc tcaaacaatc attgttgaat ctaaccatga tctagcgtta tctcgttggc 79620
tggatgatcg taatgctaac atcaaagacg atcccgcaaa cgcggaattg tatcaccgtc 79680
taaacgctgc gatttacgcc gctattgctg aaaaggatga tacctttaac gtgctagatt 79740
acgccttgcg gaacgtcgca ggatgcgaat ttaacgctat tttcctgact actgaccagt 79800
cgtttaaaat cgctggcatt gaatgcggcg tacatggtca caatggcatt aacggcagtc 79860
ggggcaatcc taagcagttt aagaaacttg gacgccttaa cactggtcac actcacacgg 79920
ctagcattta cggcggcgtt tataccgctg gcgttgctgg ttcccttgat atgggctaca 79980
acgtgggcgc gtcaagctgg acgcaaacgc atttaattac ctatgcaaac ggtcaaagaa 80040
cattgattga ctttaagaac ggtaaattct ttgcataatt aaatttaatc ggcggttgaa 80100
atataccgcc attaataaaa ggtttattaa atgaatattg tattacctaa aacactggat 80160
aaaaagcagt atataacgcc ggatgatatg gaatgcggca aggtatacca attcgattat 80220
atgttggcta gtccgacgaa tacaggttgt attattgcag ttgatacccg tgataatgac 80280
gtattaacgt ttaatgatta ttctgacttt atggaatggt tagatgatca aagtattact 80340
aataacggcg aattaacgcc gtatcaatta acaattgcaa ttaaataaat taatcccgta 80400
attaaataat ctggagtcat aaacatgaaa aaccaaaaca ttccttttga tcgtgctact 80460
tcttccatcg ttttagttta ttctaatggt gaacgttatc acgtcgaggc ggggcaagtt 80520
aattttgatt tgctgaatta taacgacgct ttgcaggtta ctacctttgc ttatgatacc 80580
ggcaaagtgg caagccatac aaaagcaaaa ggcgtttacg ctgatacttt gacagtgcaa 80640
acaattatta ttgatgcgct taaatccggt ctagcgtttg cagttgttaa gccgtgtccg 80700
gtctgcaatc ccgaagctag cgcgatggtt tacacttgcg cggggattcg ctctagcgta 80760
catggtgaag atcttagctt tatcgccgat gctctcgctt ttggtctggc gttctaatag 80820
ttaattgaac attgccagct aatctaatat aattagctgg caagattgaa ttaacctaaa 80880
tcccttaaat ggagaatgta acaaatgaat caagtcaata tgaatattac ccgcactttc 80940
ccgcatattt cccgcgttat gatttgggat ttagacggta caatcattaa ctcaatgcac 81000
cgtgtcgggc cttgttttga tgataatggg aatttagatt taaataaata ttcccgcgaa 81060
gcctgcaagc atgatttaat tatgcaggat tctttattgc cattggttga atatatgcgc 81120
caatgtatga atgatcccaa taccttaaac cttatttgta ccgctcgctt aatgggtaaa 81180
tctgattatt atttcttgcg taaacaaagt ctaagagggc gcggaaatag taatattcgc 81240
gtattttccc gcgatacatt acataaatac tttgaagcgg gtaaggttag cgaaatatat 81300
cacagcaaag acgctgtata taagtcttat tactttgaac tgtttaaaca gctatatcct 81360
aacgctgatt ttacgatgat tgatgatcat aaaggtgtat tatcagcggc ggcaagtgct 81420
ggatttaaaa cgctggacgc tcaagccgtt aacgatatcc tatcaatcgg cgtaacattg 81480
atcggtgaaa cctttattga tgaatcgctt gatgatgata acgattatca attcctagcc 81540
gaacgtttaa aaatgtgttg ggaaggtatg accgaggaag aacgcgcgga atatagtacc 81600
acaccgcaac aattcattga taaattaaaa gttgcctaac aattaaaaat aaaatagtca 81660
cactgtgtaa tgataacatg gtgtgactat tagcttgcta agtaaggggg gcggtaatga 81720
gactcattct catctaggga acctctgggc acaccttcat gtgtaatctt atgagatttc 81780
gagaattggt aattagcgct aacttaggag gaacaccggt ctactggctt gtctcctgat 81840
tctgacatac gaaatctcct ccgcgatgcc cgagatccat ccgaccactc tcgctcaaac 81900
tcctcccgtt tctccgcaaa cttccgctcc ctttccgaaa gctcccgcga tactcccgca 81960
atatattcac gatgcttatt aatcgccccg agtgttccga agattcccgc aagcaccccg 82020
ataatgaaga tagctacaaa gctaaacatt atttatctcc ccgcagctta cgttcgatac 82080
gagctaggtg gttatctaaa atgcactgcc cgataattac cacaactagt aaagcaataa 82140
tcactacttc cattataacc accccgccat agctgctaac caaaagagta caaagataaa 82200
gaataataca gttccgaatc cgaaactatt tatccaactt tcgtacactt ccccgcgtct 82260
gttctttctt ctgttgtcgt ctgacatctc tttgcttctc ctgtagtcct ggtatttgga 82320
atatcctagc aattgcacga accattatga ctgtcccgat aagatccatt acttcatcga 82380
agccggactc ttctagtaag aaggatgttc ccatcacgtc ttctattgta tcttctatga 82440
cgtcttgaat gatttcgtta atcgcatcat gtattgcttt agtcctggga tctatttcaa 82500
agttaacagg ttcctgaaag gacttttcta catcttgcat tgtgtttgct acctgtacct 82560
catcccagaa ataaaaattt ttgtttactt cgagatcttt ttactttata ctaattacaa 82620
gtgaccagat tgttctagtt taaatttgca atcgccatat ttgagatcaa actgtgacaa 82680
atcccaccac ccaaacttag tgcgtaactg agttttaggg aatcggtagc ctacagtacg 82740
tgcttctcga atatctaaag catagtgttc ttcaccaaca tgaccataag acttcacata 82800
tgccttacaa aatggctcgg gagctggtac acaacccgtc aacactactg ctgcaactac 82860
gatagctagt aactttttca taatcgttcc tctgtttaat ttataaatat attatagcaa 82920
aatagaggga tctaagcaaa tacattttta aagaaggaga ttgtatgagt tttttcgcag 82980
gaaaatttag tgatggtaaa actgtattat cgctaaacgc tgctaatggt ggtgatactg 83040
gtgaacacta ctctccaaat actaatagta tctttcactc agatatgcct ttcgtcctgg 83100
ttgatgggac ttatgaagcg gctctaggta atgcaggtaa tggatacttt gttactcaga 83160
tgccttggga tattataaat attaaatcca atgatccggg tagagttata ttaactgcta 83220
ttgagattaa tggtacccat agaggattcc tcaatggtac tcaatctcag gttggccagt 83280
tcttatcatt ctttgaagac ccaccacgtg ctggtgctga attatctgta acatctgctt 83340
ttgcgatcgg tgatagttta gctcatggta cctatatcta taatggtggt cttggtcatg 83400
aggaatctat tgctcggcaa ggttctggag gcagtttact acagtcctct ggtttccaga 83460
tctttagacc tgggggtgtt tctgctggtg aagccattgc cagagcctgg agtcttatgg 83520
gttttccaac cggtgtctct actgttcctt tagatggtgg taacgcagat tattgggaac 83580
ctaactggca ggcgcctatt ggttctgctg gtagaggcca tgattggttt tatgtttgta 83640
gttctaatat tcgtggcttc gcaggtaaaa aaggcgtact accagataac gtaaacgttc 83700
tttaccaatc tcctgcatac ccagaaaaaa tatatgtttg tagaggttct acttctaata 83760
tggctgctca gtctgctaga aaggtatatg tacaggactg gtataacgtt acgccaacta 83820
aggttatttg gtatgtgcta aatctgcgct attctaatgg tagtatggga gtttctggta 83880
atccatttac cggttcagat atcctaatat ctccatctaa ctttactatt aaaggtgtta 83940
gcctaccaaa tacaggctat aaatttatta accagaatgc tttcggaaac ttaggctatc 84000
gccctgatat ggagtatgtt ggtaataacg ctgcgtatac cggagttttt ggtgataata 84060
ctgcacgatg tgaatttatc ggctctagta atggcgggtt gtggtctcct gttaactatg 84120
ggggtgcagc ttctcaaatt agcttatata aatttggtgt aggtaaacaa tggtatgtag 84180
atactaatac caactccatt ggtaatgaac atggacctgt gtggagtcct agtactgtac 84240
ccctcaggtt attcccaggc aacgttgcaa gtacttatgt aggggacgat ataactccat 84300
cctaccctgg tgctggtgac ttctacacac ctttggccac agtttggttg ggattaccaa 84360
atgctcactc tactgttata ttaactactg aagttattat gggtaatctt aatactgctg 84420
gaatgcctgt gcgtacttac ggcggtagcg cttggcaggt acaaggtaga cgtcaacaaa 84480
gttatacagc tggtgatggt gtattccacc agattcttac tctgccacct ggatatctag 84540
taccttatca cagtacaact tcctatagtt atacacaaac ttgggctagt aggcctgatg 84600
agtttgcttt ccgtaggaac ggatatattt atactattaa aaaccttggt aatggcaacg 84660
ttgagttggg tgttatcatt cacgctgcag aagcggcagc tgttttcttg ccaagactac 84720
gtgtaacagt tcaacgccta acctaaagga ggatatatgg caaatgatgt attagttccc 84780
gatctcatgt ctcccgaagg catggatgtt attgaagcct atttacagtg cggtagtgat 84840
gttcctgccg ccgctcgtag tcttggaatg tccgagattg cattccgtga tataatgaat 84900
cgtggggaag ttaaaaacta tctcaacgat atctttatgg aaagtggctt ccgtaatcgt 84960
gaccgcttat tcggagttct tgatgaagtt atcaagcgca agttggaaga actcgaagaa 85020
actggcatgg gttctgacca ggatatcatg gatattctct ggaaggcgca caagatgaag 85080
atggaagaaa tgaaaatgat ggtagaactg gagaaagtga aggcagcagc tcgtactccg 85140
gctaaccaga ctaacattca gaataatatt attgctggaa gtggtgacga aaactatatg 85200
aatcttatta ccgctctagc aggaggaggt aagagataat gaaagtctct agagactata 85260
ttaacaccac agatgttatt gactttggta ttgacaaacg atttttccgt ctgccggtct 85320
ctagcattct agctcaagag gggattacac ctaatgcccc tcaggttgca attattaacg 85380
cgctggaaga tcctagacac cgtttcgtga ctgcatgtgt atcacgtcgt gtaggtaagt 85440
cattcatcgc atatacactt gggttcttaa aacttctgga acccaatgtg aaggtgctgg 85500
tagttgcacc taactactca ctggctaaca ttggttggtc acagattcgt ggtcttatta 85560
agaagtatgg attacagact gaacgtgaga acgccaaaga taaagagatt gaactagcga 85620
atggttctct ctttaagctg gcttctgcgg cgcaggctga ctccgcggtt ggtcgttcat 85680
atgacttcat catttttgac gaagcagcaa tttccgatgt tggtggtgca gctttcgata 85740
ttcagttacg tcctacccta gataagccaa actctaaagc tctatttatc tctactcctc 85800
gtgggggtaa ctggttcaaa gagttctatg agaaaggatt taacgaaacg ctacctaatt 85860
gggtatctat tcatggtaca tatcgtgata atccacgtgc tgacctgaac gatatcgaag 85920
aagcgcgtcg tactgttagt aaaaactatt tccgtcaaga atatgaggct gacttctccg 85980
tattcgaagg tcagatcttt gataccttca atgctatcga gcacgttaaa gacctcaaag 86040
gtatgcgtca cttctttaaa gatgatgagg ctttcgaaac attacttggt atcgacgttg 86100
gttatcgtga ccctacagca gttcttacta tcaagtatca ctatgatact gatgtttatt 86160
acgtactaga agagtatcag caggctgaaa agactacagc tcaacacgct gtttacatac 86220
agcactgtat agatcgttat aacgttgatc gtatatttgt agactccgct gctgctcagt 86280
tccgtcagga cttggcatat gaacatgaaa tcgcttctgc tcctgctaag aaatctgtcc 86340
tagatggtct ggcatgccta caagcactgt tccagcaagg taagatcatc gtagatgctt 86400
catgtacctc attgatccac gccctagcga actacaagtg ggacttccag gagggcgaag 86460
agaaattatc acgtgaaaaa ccacgtcatg atgcgaactc tcacctttgt gacgcactgc 86520
gttatggaat ttactcaatt tcccgtggga aataaataag tataggatgg gatactactc 86580
agttggtatc ccattcctgc attttaaaat cccctttaca aattcgacat gattatgtat 86640
actatattca tacagttgag gagaatactg tggcaggaaa tgtaaaatat aaacgtgatg 86700
ctatatcact catgcgagac ggtataaaaa gtcaatataa aaaagccgat cgctgcgaaa 86760
tctgtggttg tggtgaagaa ttagagcttc atcattacca tacagtgtct ctactagtta 86820
aaaaatttgc taaagaactc caattggatt tcactgacga agaaacagtc ctctcaaata 86880
ggacggcatt ctatgaaaga tataggcatg agctagtaga tgacactgtt accctttgtg 86940
tccaccatca ccaactatta cataaggtgt acactaaaga acctcccctt ttctctgcta 87000
ataagcagaa ggcatgggtt cttaaacaaa aggaaaagct acagaatcct caagaaaaga 87060
cacaagtcaa gactgaaact aaatcaggat tcgcaaggtt cttataatgg gtttaaaaag 87120
ctggattact gaaaagctaa atccgggtca acgtattata agagacatgg aacccgttag 87180
tcatcgcact aaccgtaagc catttaccac tggacaagcc tacagtaaga ttgagattct 87240
caaccgaact gctaacatgg ttatcgatag tgcggcggag tgttcttata ctgtcggaga 87300
taaatataat attgtcacgt acgctaatgg cgtcaaggca aagactctag acactctctt 87360
aaatgtacga cctaatccat tcatggatat aagcacattc cgtagacttg tagtcactga 87420
cctacttttt gagggttgtg catacatcta ttgggatggc acagcacttt atcatgtccc 87480
tgctgcacta atgcaagtag aggcagatgc caataagttt atcaaaaaat tcatatttaa 87540
taaccagata gactatcgcg tagatgagat tatctttata aaggataaca gttacgtgtg 87600
tggcacaaac tcccaaattt ctggacaatc tcgtgttgct acagttattg actctcttga 87660
gaagcgttct aagatgctta actttaaaga gaagttcctc gataacggca cagtgattgg 87720
tcttattctt gagacggatg aaatcctgaa caagaaattg cgtgagcgta aacaagaaga 87780
attacaactc gattataatc ctagtacggg tcagtctact gtcctgattc tagatggtgg 87840
tatgaaagca aaaccgtact cccaaatatc ctcttttaaa gatctagact tcaaggaaga 87900
tatcgaagga tttaataaat ccatctgtct tgcctttggt gttccgcaag tgctacttga 87960
tggtggtaat aatgctaaca ttcgaccaaa catcgaattg ttctattaca tgactatcat 88020
tcctatgctg aacaagctga ctagttctct tactttcttc tttggctaca aggttactcc 88080
taacactaag gaagtagctg cactgacgcc agataaagaa gctgaggcta aacatttaac 88140
ctctttggtt aataacggta tcatgactgg taacgaagct cgtgaagagc ttaaccttga 88200
acctttagat gatgagcaga tgaataagat tcgtattcct gctaacgttg ctggttctgc 88260
aacaggtgta tctggtcaag aaggtggtag accgcaaggc actgccgatg gggataaaga 88320
atgattgatt atgatggtct aaaggccatt tttggtgaaa agctgccaga atctcatatc 88380
ttctttgcta cggttgcagc acacaagttt gttccaaact acgctactct acgtaaagag 88440
tttggaatga ccactgcaca taccaaccgc aaggtttgga ataagttcaa agaagcatac 88500
gaagttactg ctccggttgt tccggctcta gctttcacga agaatctggc taaaactcta 88560
gctgttgata ctggtgcagc aattaatctg ggcgtaacag ttactggtgg tactgcacca 88620
tatacttatg cttggactaa agacggttct ccacttagtg gtgttactgg ccctaatttc 88680
aacaaagcta cagctgctgc agaagatgct ggtacttaca aagttgttgt aacggatagt 88740
aaatctactt ccattacttc taatgagtgt gtaacaacta tcaaccctgc gccggaggct 88800
taataaatga caaatgctgc tattgactat aacaagttaa aatcagcacc ggttcattta 88860
gacgcttaca ttaagtctat tgatagcgaa tccaaagagg gtgttgtaaa aatccgtgga 88920
ttcgctaata caattagtaa agatcgcgct ggtgacgtaa ttcctgcttc ggcatggaaa 88980
acgtctaatg cacttgctaa ctacatgaaa aacccgatta ttcttttcgg ccacgaccat 89040
cgtcgtccaa tcggcaagtg tattgatctt aaccctactg aaatgggtct cgaaattgaa 89100
tgcgagattt atgaaagttc tgatccagct atcttctcac taattaaaaa cggtgtactg 89160
aaaaccttca gtatcggatt ccgctgccta gacgcagaat gggatgaagc tactgacata 89220
tttattatta aagatttaga actatacgaa gtttcggtag tttctgtacc ctgtaatcag 89280
gactcaacgt tcaacctcgc taagagtatg aatggccacg attacactga atggcgcaaa 89340
tcttttactg caacaagttc taaggctgtc ccagctcaag aacgtaatct ttctgaacta 89400
gaaaaacttg cgatagcttt aggctacgtt aaagaataac ggagaattat ttaaaaatga 89460
ctattgatat taataagctg aaggaagaac ttggtctggg tgatctggct aaatctctgg 89520
aaggtctgac tgctgctcag aaagctgcgg aagctaagcg tctgcgtgaa gagcaggaag 89580
aaaaagaact ggctcgtatg aacgacctag tatctaaagc tgttggtgaa gaccgtaagc 89640
gtctggaaga agctctggat ctggttaaga acctggatga gaagtctaag aagagcgcag 89700
aactgtttgc acagactgta gaaaagcaac aggaaaccat cgttggtctt caggacgaaa 89760
tcaagtctct gttggcagct cgtgaaggtc gtcccttcgt tggtgatagc gtagctaaag 89820
ctctttatgg cactcaggat gctttcgaag atgaagttga gaaactggtt cttctgtctt 89880
atatgatgga gaaagacgta ttcgaaactg aacatggtaa ggctcacgtt aaagctgtta 89940
acggttcttc ttccgtttcc atgtctagcg aagcatacga aactatcttc tctacccgta 90000
ttatccgtga cctgcagaag gaactggtag ttggtgcact gttcgatgaa ctgccaatgt 90060
ccagcaaaat ccttactatg ctcgttgaac cagaagctgg tcgtgctact tgggtagatg 90120
cttccaaatt cggtactgac gaaactgttg gtgacgaagt taaaggtcaa ctgactgaaa 90180
tcagcttcaa aacttacaag ctggctgcta agtccttcat cactgatgaa actgaagaag 90240
atgcaatctt ctccctactg cctctgctgc gtaagcgtct gatcgaagca cacgctgttt 90300
ctatcgaaga agctttcatg tccggtaatg gtactggtca gcctaagggt ctgctgaaac 90360
tggctgctga tgatggtgct aaggttgtta ccgaagctaa agctgacggt tctgttctgg 90420
taactgctaa gactatctcc aagctgcgtc gtaaactggg ccgtcacggt ctgaaactga 90480
gcaaactggt actgatcgta tctatggatg cttactacga tctgctggaa gatgaagaat 90540
ggcaggatgt tgcccaggtt ggtaacgatg ctgttaaact gcagggtcag gttggtcgta 90600
tttacggtct gccggttgta gtttctgagt acttcccagc taaagctgct tccgctgaat 90660
tcgcagttat tgtttataaa gataacttcg taatgccgcg tcagcgtgcc gttactgttg 90720
aacgtgagcg ccaagctggc aaacagcgtg acgcatacta cgttacccag cgtgttaacc 90780
tgcaacgtta cttcgaaaac ggcgttgtat ctggtgctta cgctgcatct taatacaggc 90840
ttttcagccg ataaggagag cttcggctct ccttttttat tgggaaaaat aaatgcaaat 90900
catcactgtt gaagactaca gactatatgc taatgcaaag cgacctgata tggaaactca 90960
ggttgagatg atgattaaag ctgccaatac gctgattaca agccttctag gtatggatga 91020
tgcagatgcg gtagatcaat taatcactac aaaacctagc cgtaggaaat atttcctgag 91080
ttctccctca gccacatcta ttacaaagat gactattaac gagaatgaaa tagacccgga 91140
acagtttaaa ctgtatcctg atggtgttat tctccttaag tttaatcctc ctgaaggata 91200
tatggatgta gagtatactc aaggtggttt caatccaatg cctgaagatc ttaaactggc 91260
agcatgtctg ttagtagatc actggcataa gcaagattat cgtcaagcca agactattgg 91320
tggtgagaca gttacgttta acaatactaa gtctggtatt ccagagcata ttcgtacaat 91380
catcgaagtc tacaggagag tataatggct ctttctgatc tagctagaca aattattaaa 91440
gagcagctag atactgcagg acgatctgag aataataaga atactgttgt gtatacagtt 91500
gagacaggtt tgaaagatcc tacaagagat ggtacagttg cacaggtatc tttcaaattc 91560
tcgaaaccag tatcacaaga cttattaaat gttaggacag catctatact gaaggctgtt 91620
tcttctaact tagatctttc tggtgattta ggtgctctag agaatctaat tcaagcaacc 91680
gcaggtaaac gttccgccgt tggtaaaaaa cgttctaccg gtagagttca ggtaaacttt 91740
ggagatccta gagatactga agatggctat tctggtgcag taacaggtgc ttccggtcgt 91800
ttcgtatcga atagtaacat gcgtgctatt cttgagatag tagctaagga atacttaatc 91860
aaggatatga aaaaagcagg ggcaccattg aagttcagaa ctggtcgctt tgctaactcc 91920
ttaaagatta aagacgttat gctacgtgat gcagaaacta gtaaaggtgc tccagagctg 91980
aatgtgacgt ataactatat ggttcgtcct tactctgtgt tcaaccccgc agtttctaca 92040
tatcgaagac tttctctacg accttaccct ggcgctcgaa accctcaaaa acttattggg 92100
gaagcaatcg caaaagctgc aagagacctg attcactcta gatataaaat caaggttaat 92160
caaggaacct agtaaatgga tcacagaaca agtattgcac aagcagtggt tgaccgaatc 92220
gccacacaaa tggatggttc acaacctgac gagtatttca ataaccttta tggaaacgtt 92280
tctcgtcaaa cttataaatt cgaggaaatc cgagaatttc cttatgtggc agtccatatc 92340
ggaactgaaa ctgggcagta tcttccctca ggccaacagt ggatgttttt agaacttcca 92400
atcctcgtgt atgacaaaga gaaaacagat attcaagagc aacttgaaaa actcgtagct 92460
gacataaaaa ccgtcattga cacaggtgga aatttagaat atactgttag taaacctaat 92520
ggatcgacct tcccatgtga ggcaactgat atgagcatca cctcagtagc tacagatgag 92580
ggtctactgg ccccatatgg tttagcggaa ataaatgtaa cagtgaggta tcagccacct 92640
cgtagggcac ttcgcagata agttacagat taggagaata taaataaatg tctttacaac 92700
tattacgtaa tactcgaatc ttcgtgtcta cggttaagac tggtcacgat aagaccaaca 92760
cgcaagagat tctagttcaa gatgatattt cttggggtca ggacagtaac tcaaccgata 92820
ttactgttaa cgaagctggt ccgcgtccaa ctcgtggttc aaaacgtttc aacgactccc 92880
tgaacgcagc tgagtggagt ttctctactt acatcctgcc ttacttagat aagactacta 92940
gtaagcagat cgttccggac tacatgctgt ggcatgctct ttctagtggt aagcctatca 93000
acctagatgg cgatacaggt gctcacacta acgaaactaa cttcatggtt aactttaagg 93060
ataacgcata ccacgaactg gcaatgctgc acatttacat ccttactgat aaagcatgga 93120
gttatatcga ttcctgtcag atcaaccagg ctgaagtaaa cgttgatatc gaagatatcg 93180
gtcgtgtaac ttggtcaggt aatggtaacc agctaatccc tctggatgct gctccatttg 93240
atcctgatac tgtaggtatt gatgatgaaa cttacatgac tatccagggt tcttacatca 93300
agaacaaact gactattctc aagatcaagg atatggatac tggtaaggca tatgacattc 93360
cgatcactgg tggtagcttt acaatcaaca acaacatcac ctatctgact ccgaacgtca 93420
tgtcccgtgt taacatccca atcggttcat ttactggtgc attcgaatta actggttctc 93480
taactgctta tcttaacgat aaagctctag gttctatgga tctgtacaaa gatctaatca 93540
agaccctgaa agtagttaac cgtttcgaaa tcgctctagt acttggcggt gaatatgatg 93600
atgagcgccc agccgctgtt ctagttgcta agcaagctca cgttaacatc ccaactattg 93660
agactgacga tgtacttggt acttctgtag agttcaaagc aattcctact gatctggata 93720
ctggtgatga aggttatctt ggtttctcta ataagtacac caaaactact attgcaaatc 93780
tgattgcaac tggtgacggt ggtaaagcag ctccggttaa ggtaacaggt attactgtta 93840
aatccgcggg tgatgcagct tccgttgata atagtgcaac tcttcagatg actgtagatg 93900
ttttgcctgc tgaagctact aataaggaag taacttggtc tatttcctcc ggtactgctg 93960
ctactattga tgcaacttct ggtttactga ccgctgatgc ttctaagact ggtgcagtta 94020
ctgtgaaagc cgtagctaaa gatggttctg gtgtagaagg tactaagagc attacagtta 94080
ctgcttctgg tggctaatta aatgtactac tctctaatga gggagtcaaa agttatagtt 94140
gagtatgatg gcagggctta tcattttgat gccctgtcaa attacgatat caatacgtcc 94200
tatgaggaat ttaaaactct tcgtaggact atccatcgta gaaccaacta tgctgactct 94260
attattaatg cacaaactcc atcttcaata tcgctggcta taaattttag taataccctt 94320
actgaagcta acttctttga atggatgggc tttgaccgag agggtaacac attcttacta 94380
ccactttata gtacaaatat tgagccaaca atgtttaata tatacatagt taataaagat 94440
aataactgtg tgtactttga gaattgctat gtatctactg tagacttttc tctagacaaa 94500
agcgttccta tcctaaacgt aggaattgaa tctggtaagt tctctgaggt gtctactttt 94560
actcctgctg gttctattgt tcagggtgaa gttatgtcat atagtcctcc tagagtatct 94620
accaacagta atgttttacc agctctttta tccgcttcta tgtcattcca gcaacaatgt 94680
tcttggagag aagataaaag cgtatttgac ataaacaaaa tatataataa taagagagct 94740
tatgttaatg aaatgaatgc ttcggcaaca attgcatttt actacactaa gcgatttgct 94800
ggtgatatgt tttataatat tgaacccgaa actgacatac cattaaccat ccgaaatgaa 94860
catatttcga ttgactttcc ttcagcacgt ataacaaaac gcctacaatt ctcggatata 94920
tacagagttg aatgggatgt aatccctact gcagactctg atccggtaag aatcgacttc 94980
tttggagaaa taaagaaatg atcaatttaa aagatattac ccttgaaact cgtactattg 95040
agctggctta cccaggtatg cctaacttca agctgcaact gaactacatg tctcgtgctg 95100
catctaagcg tgttatctct tccgctaagc gtgatgagtg ggttaacggt actatgattc 95160
aggttcagga cgatgataag tttattgaag ctttcgttga tactgcaatt gctggctgga 95220
ctggcttgac gattggagat gttgagaagc tgatgcttat cgaaactgaa gctgatccta 95280
ctactgaagt tccgtttagt cgcgataacg ctatcatgct tatgcagaac tctgctgcgt 95340
ttgattcttg gatcaaccag acagtgttcc acttagacac ttttcgtagc tcaaaagcgt 95400
aagcagttac tagatgctgt tgccgatttt gcagataaat gcattaaatc ctcagcatct 95460
aaaatgacaa aacaacaata tttaatgctt tgcgagtctc taggtattga acctgatcct 95520
aaggcaatgc ctgttgaact cgaagatttt ccacctattg tatctactag tatgaatatt 95580
tataatagtc ttattgactg tttcatacca ggtgactttc ctatctttat aggaaaagac 95640
aaaagtgcat taggtgtttt atttgatatc tatgggatta ctgatcctat agagaaagag 95700
tttgttctac acatcatcaa tatatttgat tcgaaagctg tagatgcagc tcgtaagaga 95760
gccgagaagc atagtaaacc taatggtcgt accccgaaca ttaaaccaca tgctagatct 95820
cgagctcagt aaaagtttcc tccaatgggc gttccacgat gaggcttggc tctgggtgat 95880
ttgcccagag ccttttttat tgggaaaaat aaatgactga taagctaata cgagaactac 95940
ttatagatgt aaagcagaaa ggggcaaccc gtactgcaaa atctattgag aacgtttccg 96000
atgcgttaga aaatgctgct gccgcttccg aactgactaa tgagcagtta ggaaaaatgc 96060
ccaaaactct ttactccatt gagagggcgg cggatagagc agctaaaagt ctaactaaaa 96120
tgcaggctag tagaggcatg actggtatca ccaaatctat ggatagtatt ggtgctaagt 96180
tagatgacct tgctattgct atgatcgagg tatccgataa gctagagtct ggatttacac 96240
acgttggtaa atctgttaag gcgatgggta acgatgtagc tgctgcaact gaaaaagttc 96300
aagatagatt atatgatact aaccgagcac taggtaatac tgctagaggc tttaatgata 96360
caactaccgc tgcaggacgt gctagtcgtg caattggtaa tacttctggt tcagcacgtg 96420
gtgcaactcg tgactttgca gctatggcta aagtgggtgg tggtttacct cttatgtacg 96480
ctgctatcgc atctaacgtg ttcgttctac aatctgcatt cgaacaactt aagatgggtg 96540
accagttaaa ccgtcttgag aagtttggta ctattgttgg tactcaaaca ggtactccgg 96600
tacaaactct agctagatct ctacaagagg ctgctggtta tgcaatctcc ttcgaagaag 96660
caatgagaca agcatcctct gcttctgcat atggattcga tgctgaacaa cttaataaat 96720
ttggtctagt agctcgtcgt gctgctgctg ttcttggtgt tgatatgact gatgcactta 96780
accgtgtaat caagggtgta tctaaacaag aaatcgaact tctggacgaa cttggtgtta 96840
ctattcgtct taatgatgct tacgctgact acgttaaaca gttaaatgct gctaacacag 96900
gtattacgta taacgtcaat agtctttcca ctttccaaaa gcaacaagca tatgctaacg 96960
cagtaatcgc tgaatctact aaacgtttcg gctacttgga tgacgtactt cgtgcaactc 97020
catgggaaca gtttgctgct aatgctgatg ctgccctaag aaaggttcaa caggctgctg 97080
ctaagtattt agggccggta attgattcta tcaacgcagt attctatact tctcaagcat 97140
ctatctcggc ttctgctgct agagcacagg aagaaactaa ccgtcagatt gacccaacta 97200
atgttggtgc tgtagcttta agtctttccg cttctgagga aggatataat aaagctctgg 97260
atatgtataa agaatcccta gaaaagcgta ataagttaaa agctgatcta gacaagcgta 97320
tggaacaggc tgacttcttt accgcaggtg caattcgttc tgtagctcaa ggtgtccctg 97380
tgagcgctgc tgctcttggc gcttcagagg aaaacaagaa gttcgtagct gaaactgcgg 97440
ctatggcact acaagtagaa cgtttggata aggaagttac tgattcaacc gataacttag 97500
gtgcatggaa atctgcatat caagctgctg gtgctgcggc tgctaaggct aatccagaat 97560
tccagaagca gattaatctg cagaaggata tgaatgaccc tgatgcggtt tatgacttta 97620
actctgcaac tctgaaagga ctgacagaac agcagaaagc atatgatcag gctaagaaaa 97680
ctgctagtga tttagctaac gatatccaga acatcgctca gaatactaat actgcggcta 97740
aaactagtgc atccctatca gatacgatta agactattga gtctctatct gctggtactg 97800
gtaaaaatgc tgatgagtat gttaagagcc tgaacttagg gtataatact cttagtgaga 97860
tgaagactgc ttcacaagct cttgccggtt acgtaaaatt aactggtaat gagactaaga 97920
atcagttaga agttcagcag aagattgccg aagtgtacaa ccagactaag gacaaagaga 97980
aagctcagga agctggtaga cgtttggaaa ttcaacagtt agaagaacag gaagcagctc 98040
ttaagcgtgt cctagaaact aacaaaggta acaaagcaat cgaaggtgaa atagctaaga 98100
ttcagttaga acgtatcaag attactaacc agggtatgga agctcagaag aaggttaagg 98160
actataccga taagattctt ggtgttgatc gtgaaattgc tctcctgaat aaccgtacta 98220
tgacagatac tcaatatcgt ttagctcagt tgaaccttga actgactgtc gagaaagaaa 98280
agtatgaatg gtactccaaa cagattgata agcataagga agctgaacaa tcaagacgtg 98340
ctcaggcaca aattgaacgt gaaatctgga aattccgtca ggatcagcaa agggaaatgg 98400
ctgctggcag ggaggaggag caacaaagga agtttaccgc aaatccttta atgggagctg 98460
cggaacgtat caaggagcaa atccagctat tcgaagacct taagcaaaaa actttaggga 98520
atgctgctgc tcagttagag tataataaaa agctagcaga aactagagcc cagctcgcag 98580
ctttaagagt acagcgcgat gcagaaatgc agtcttctgt aggtgcggct gtaggggcta 98640
cctatactcc tactactgga ttaactggag aagataaaaa cttcgcagat atgcagaata 98700
gaatggcgtc ctatgaccag gctatttcta agctgtctga actgaactcc gaagcaactg 98760
ctgtagcaca aagtatgggt aacctaacta atgctatgat ccagttctct caaggatctc 98820
tggatactac ctctctagta gcggctggta tgcaaactgt atcttcaatg attcagtata 98880
gtgttggtca acaggtaagt gctattgatg cagctattgc agcagaacag aaacgtgatg 98940
gtaaatctga gcaatctaaa gctaagatca agaagttaga ggctgaaaag ctcaagatcc 99000
agcaagatgc agctaagaag caaatcatta tccagacggc agtagcggtg atgcaggcag 99060
caacagctgt tccgtatcca ttctctattc ctctgatggt tgcggctggt ttagcaggtg 99120
ctatggctct tgctcaagca tcttctgcat ctggtatgtc ttctattggg gactctggtg 99180
ctgagacagc tggttactta actcttggag aacgccagaa gaatgtagac gtttctatgt 99240
cggctaatgc aggtgaactt tcttatattc gaggagatca agggattggt aatgctaact 99300
ctttcgtacc tcgtgcagaa ggtggtaata tgtatcctgg agttagttac cagatgggtg 99360
agcatggtac cgaagtaatc actcctatgg ttccgatgaa ggctacccct actgatgaac 99420
tcaaaggatc ttcaaaaggc acatcgggta gacctattgt gctgaacatc agtacgatgg 99480
atgcggctag cttccgagat tttgcttcga gcaatagtgc tgctttcagg gatgccgtgg 99540
aacaggctct taatgagaat ggaaccaccc tgaaatcact tggtaattct taatactgga 99600
ggaggacttt atgtcctcct tttctttatg gaaaaataaa aatttcttga taaaattttc 99660
caatcctatt ataatattgt tattaagagg agaaattaac tatgagacta ccagacccat 99720
acacgaaccc agagctatca gggttaggtt ttgaaagcgt taacttagta gacaatgacc 99780
ctatgattcg tgatgaatta cctaatggta aagttaggga ggttaaggtg tctgctcaat 99840
attggggtat taatatctct tatccagaat tattcccaga tgaatttgct aaattagatt 99900
cattcattct agattacaaa agaacaggta gctatattga tgtgttatta ccgcaatatg 99960
aagctttccg agttcgtggt gatactagtg ctgtaaatat tcctgctgga caaaaaggtt 100020
caacagttat tatggacact aaaggtacac ttcgtggact gcctaaacca ggcgatttat 100080
ttaaattgtc caaccaccca aaagtgtaca aaatcacttc tatcaacaca gtaggaaata 100140
cttggaatat aagtgtttac cctgatctat tcattactac tacgggtagt gagaaaccgg 100200
tatttaatgg tattctattt agaactaaat taatgaacgg agacagcttc ggctctacgt 100260
taaatagtaa tggtacatat actggtattt cactttcctt gagggaaagt ctatgaagaa 100320
aattcttgac agtgcaagag agtatttaag aactaataat aaaataaaaa ctgcatgcct 100380
tattactctt gaattgccaa gctctaccgg atcaagttct gcatacattt atcttacaga 100440
ctattttagg gatgtaattt ataatggtat tctttacaca tccggtaaag ttaagtctat 100500
aacaacacat aaacaaaata gaaaactttc cattggtagc ctttctttta ctatcacagg 100560
tactgctgag gatgaagtat taaaactagt ccaaaatggt gtatccttcc tagatagatc 100620
tatctcaatc catcaagcgg ttattgatga agaaggtaac attcttcctg tagatccaga 100680
cactaatggt cctttacttt acttccgtgg taaaatcact ggtgggggta ttaaggataa 100740
cattggtact tcgggagtta gtacatccgt tattacatgg aactgttcaa accaattcta 100800
tgactttgat agggtaaatg gacgtttcac agatgatgct gaccatagag ggcttgagat 100860
tgttgccgga caattagttc cgtctaatgg tgctaagcga cctgaatatc aagaggacta 100920
cggattcttt cacgctaaca agagtatttc tattcttgct aagtatcagg ttcaggaaga 100980
acgttataag ttacaatcta agaaaaagtt atttggtctt tctagaagct atagcctaaa 101040
gaagtactat gagactgtaa ctaaggaagt cgatcttgat tttaacttag cagctaaatt 101100
cctaccagta gtatatggag ttcagaaaat tcctggaatc ccagtatttg ccgatactga 101160
actgcataac cctaatatcg tatatgtagt gtacgccttc tgtgagggtg agattgatgg 101220
attcctagat ttctactttg gtgatgtacc aatgatctgt attgatccta atgatagtaa 101280
gtctcgtact tgctttggta ctaagaaagt agctggtgat accatgcaac gtattgcttc 101340
tggtcaacca agctctgaac cttctgttca tggacaggaa tataagtata atgatggtaa 101400
tggcgatatc cgtatctgga cttatcatgg taaggctgat caatctgctg ctgatgttct 101460
agttaacatt gcaaaagaga aaggtttcta tctacagaac atgaatgaga atggaccgga 101520
atactgggac tctcgttata aactgttaga tactgcttat gctattgttc gctttactat 101580
taatgaaaac agaacagaaa ttccagaagt tagcgctgaa gttcaaggta agaagattaa 101640
gatctatcat tcagatggta gagtaactgc tgataagact agcttaaatg gtatctggca 101700
aactctggac tacctaacct ctgaccgcta cggtgctaat atcactattg atcagttccc 101760
tctccagcaa cttattcaag aagcagctat cctggatatc attgatgaat cctatcaggt 101820
ttcttggcaa ccctactgga gatacgttgg atggactgat gctgtaggag agaatagaca 101880
aatagttcaa atgaatacta ttctcgatac ctctgagtca gtatttaaaa acgtgcaggg 101940
attaatagag tcttatggag gtgctatcaa caacttatct ggtcagtata gaataactgt 102000
tgagaagttc tcgaatactc cactggagat tgacttctta gatacctatg gtgatctgga 102060
gctatcagat actactggta gaaataagtt taactcagta caggcctcta tactagatcc 102120
cgcattaagc tggaaaacta actccattac attctttaac tctatattta aggagcaaga 102180
taaagggtta gataaaaaac ttcagctttc ttttgcaaac atcactaact actatactgc 102240
gcgtagcttt gcagatagag aactgaaaaa gtctcgttac tcacgaactc tcacattctc 102300
attaccatat catttcatag gcattgagcc taacgatccg attgctttta catacggtcg 102360
ttatggttgg gacaagaaat acttcctagt tgatgaagtt gaaaactcta gagaaggtaa 102420
gattaatatt acactgcaag agtacggtga ggatgtattc attaactccg agcaggttga 102480
taatagtggt aacgatatcc ctgatgttag taataacgta ctgcctccta gagactttat 102540
gtatacacca acaccgggtg gacaagtagg atctattggt aagaatggtg agctatcttg 102600
gcttcctagc ttaactaata acgttgttta ttactccatc gttcactctg ggcatgctga 102660
tccttatata gtacagcaac tagaaactaa tccaaatcta cgaatgatcc aagaaattat 102720
tggagaacct gctggtttag cagtctttga gattagagct gtagatatta atggtagacg 102780
aagttctcct gtaacattat cggtagaact taactctgct aaaaacttaa gcgtcgttag 102840
taatttcagg gttactaaca cagcttcagg agatgcatcg gagtttgtag gaccagacgt 102900
taagttggcc tgggatagaa ttccagaaga agatattatt gatggaatct tctacactct 102960
cgaaatctac gataacctag atcgtttatt aagaagtgta cgaattgaag atcagtatgt 103020
ctacgattat ctactgatat acaataaggc agactatgct cttcataacg aggatgctct 103080
aggtattaat aggaagttac gcttccgtat aagagcagaa ggtgataatg gcgagcaatc 103140
tgtggattgg gcatctattt aaatgatttc gaataatgca ccagccaaga tggtcttaaa 103200
tagtattatg actggatata ctatggcgta tatccagcat tctatctata ccgactacga 103260
tgttatcggt agatcattct ggcttaaact tggcgaagaa gtggatagaa gagatttcac 103320
tggaatcgac actttctttg ttatgatcaa taacttaact ccctcaacta cctatcaggt 103380
tcagggagct ttttatgact caattattga ctcagaactt ttaaatgcaa aaattggtat 103440
taacctctct aatgaaacta actttaaaac aaaagagaag ccaataattg ttgcagcaag 103500
atctgagtca gaacctgtgg atgttggggt gggcgcacca atagttgttg tggaaacaac 103560
tggtgaagca agctactgta ctattgagtt aaaaagtaca gccactgaag acagtccatg 103620
actaaatatt atattggggc tttaggttct actattaaat ttggtggagt tcctatcgga 103680
gattataaga tcagaatatc tggtcaagta actatgcctg atggtgttac agttgattct 103740
tctgggtact atgagttccc taatattcta actgtagctt ataattttgt tcctcctact 103800
gcacctatcg atattgtttt taaagctgca cgaattgctg atggtaaaga acgatatgat 103860
gttagaattg agtgggactg ggaacgtggt gctggtgcta acgttcgtga attcttggtt 103920
acttacataa actccgagga atatgctaaa accggatggg ctaaagctca aaagataaac 103980
gtgggtgctg ccagagctgc aacaattata tcattcccat ggaaagttga acatactttt 104040
aaggtatcat caattgcctg gggaccaaat aagcaagata taacagaatc agctcctgta 104100
acatttatat tgaatgaaga tactcctcta gacaacagct ttgtcaatga gacgggtatt 104160
gatgttaact acgcctttat taagggcagc atgaaagatg gggaaatctg gagacagaca 104220
ttcttaattg atgcagctac tggtgctatt aacattggtc tgctcgatga agaaggaaaa 104280
acacctattt ctttcgaccc tataaaccgt gttgttaacg ttgatggtaa agtaattact 104340
agagatatta atgctgcgaa ctttatcatg actaacttat caggtaaaga taacccagca 104400
atttatactc aaggtaaatc ttggggagat aataactctg gtatttggat gggtatggat 104460
aatacctctg ccaaagctaa actagacatt ggtaatgcta cacaatggat acgttatgat 104520
ggtactactc tgcgtatttc tagtggtgta gtaattggaa caccaaatgg tgacgtagat 104580
attggaactg gtttacaggg taagcaaaca gtatttgttt ataagttagc aacatcttta 104640
ccggccaaac cgctagaaca agattatcca cctcctggtt ggtcaaaaac tccacctaac 104700
cgtacagata tgacacaaaa tatctatgcg actacaggta cacttgatcc agttactaac 104760
aaacttcttg aaggtactag ctggtctgat gtagttcagt ggagtggtac tgaaggtact 104820
ataggacatg atggacagcg tggtcctggg atgtactcca tgggtattcc tggattaggt 104880
ggttgggatg atggacaggc taacgcattc ttccaaaata actttggaaa acccccggtt 104940
aagtatgatg ttctaacaca atttaacagt aatgctccac aaacagcatt tacccgccaa 105000
tggaacgggg ctggatggat taaccctgca atggttcttc atggaaacat gattgtcaat 105060
ggaactgtaa ctgcggataa gattgtggca ggaaatgcct tcttatcaca aatcggtgtt 105120
aatataatct acgatagaaa tgctgcgtta tcagggaacc ctgaagcata ctacaagatg 105180
aagatagacc taaatagtgg gtatatccat ataaggtaat tataatgagt acagaaaacc 105240
gagttattga tattgttgtt gatgagaaag taccttatgg tcttatcatg cagtttatgg 105300
atgttgatga cagtgtatat ccaccaacag aagttcctgt taacttaacc ggatattcac 105360
tccgaggaac tattaaatct agtttagatg agaacgccga ggttctagct aactttaaaa 105420
ctcgtgttat tgatgctgct caaggtgctg ctgcaattag tctttccgtt gcagacgtta 105480
ctaatattgg tgaaaaagca tctaaagaac gtgataagta taacccaaga caacgttttg 105540
ctggttatta tgatatccta atgactcgtg atgtaattgg ctctgaagtc agttctttcc 105600
gtattatgga aggtaaagta tacatcagcg atggagtaac tcaataatgg cacttaaaac 105660
taaaattatt gtacagcaga ttctgaacat agatgacact acaactactg ctagtaagta 105720
tcctaaatat acagtagttt taggtaattc tattagttct attactgctg gtgaattaac 105780
tgccgctgta gaagcctctg ctgcttctgc tgcggcagca aaagattccg aaattgcagc 105840
aaaagaatct gaaacaaatg ctaaggactc ggagaaccta gctgcaattt atgctacttc 105900
ttctgaaaca tctgcaactc aatctgcggc atctgccgcg gaagctgaga gacaagctgg 105960
tctgtcacaa aagagtgctg aagcatccgc tacatcggcg gaagaatcta aaggattcag 106020
gaattctgct gaattagctg ctcaaaatgc ggagaccagt cgtagacttg ccgaacaagc 106080
taaaactgcg gctcaacaag ctcagacagc tgcagaaact gctaagactg gtgctgaaac 106140
agcaaaagca ggtgcagaag ctgctgctac aactgctgga gaacatgctg cttccgctaa 106200
acaatcagaa ttaaatgcta aagaatcaga aactaatgcg gctggttctg ctgtagaagc 106260
aggagataag gctgtagatg ctactactga ggcaaatcgt gctaaggctg aagccgatcg 106320
cgcagctcag attgtagata gcaagctgga taaagaagat atatctggtt tcatcaaagt 106380
ttataagaca aaagcagaag ctgatgcaga tgttgcaaat cgtgttctgg gtgaaaagat 106440
tctagtctgg aaccaaactg attcaaaata tggttggtat aaggttgctg gtactgctga 106500
ggctcctgtc ctagaattag tagaaattga acagaaactc gtatcaatca ataacgttca 106560
tgcagatgat gctggtaacg tacagatcac tcttccagga ggtaaccctt ctttatggtt 106620
aggtgaagtt acttggttcc cttacgataa agattcgggt gttggatatc ctggtgttct 106680
gccagctgat ggtcgtgaag tacttcgtgt tgactatcct gatacttggg aagccatcga 106740
agctggtcta attccttctg tttcagaagc tgaatggcag gccggtgcaa ctctctactt 106800
ctccactggt gatggttcca caaccttccg tttaccagat atgatgcaag ggcaggcttt 106860
tcgtgcacct actaagggag aagaagacgg cggtgctatt aaggaacaga ttccttatat 106920
cactactgtg aatggaattg gtcctgctga tgatactgga gctattaagc ttccttacgt 106980
ggcaatggtc aatggaacta ttcgtccaga tgagaacgga aatctagcac taggtaacgt 107040
agtaaccaaa aacgtttgga acggtactga tggagaagtc ttactaagag gtgcttttgg 107100
tcttggtggt gctggtttaa tccttaatga gcctgatgct gtttccttct ttaaagcaat 107160
gcgtgctttc ggttcagggt attatagaaa tgataacgag agtaacttag taatccctaa 107220
gtattctgct ggattctact ctaaagttgg agatacccat acctttattt gttctgccta 107280
tagcaatggt gttgcttttg tagcttctgc ttctgataga gatttagatg aagaatctac 107340
agtacatact aatatcctat acggtactgc aaataagcct gatttaaata atgatactca 107400
aggtgtctta ggtctcgata aaggaggaac aggagcttct acagttagtt ctgccaaaac 107460
taacctagaa gtggatagaa taaagcagct agtaggagga acacatataa cctctcagga 107520
tcaaaatata gtgtttatgg ttcaggacac taaaaactgg ggggtatacg accattctga 107580
aaacaagtgg atatctttac ctgttgaaca cggtggtact ggagctactg agccagttca 107640
agctagaaaa aatttagggg ctgcaagtgc cggtattaac tccgatatta ctcagctacg 107700
taatatggag ggttggcctc ttagcattaa gaacggtggt attattactc gtaaatacca 107760
cgcagttcct tcggatgggt tttacttagg atcggaagtt ttttctgctc aggttcaact 107820
ggataattca gagaatccag atacccctcg tttagaggca ttattttacg ctgaaggtaa 107880
ctatgcccaa tctcgtactg aaagggctac agtagctgct tataggcgta ctgcagatgg 107940
ttcgttaact gctactaaat atgctaacct gtatatggat tcaggggctt ggaatgcaga 108000
acgtatgcaa gctctagggt attctaaagg ttgggacgat gattcctttg gtttcctcgc 108060
tccttttcaa gcttctgatg taacgggcaa taataatgga ttcgtaccta ttattagtgc 108120
tatgactcaa tccacaggtg gctacccaat gagggctact actggattaa tatctcgagg 108180
tactagtact tggcctgcct atgtatttag acttcgtgga gattctaatt ggggatgtac 108240
ctatcagttc cacatgacag gggatattga cggatggggg tcagactaca ataacgttgt 108300
ctttaacttt acttacacaa aaaatgcggt gtcggatatc aatctaaaag ataatattca 108360
agacgtatcg ggtgaagaat ctcttgaaaa cattgaaaaa atggagttca agaaattcac 108420
ctataagttt gataagaaaa agagtattag acgtggtgtt attgcacaac aaatagagtt 108480
gattgatcct caatatgtta aagctattgg caatcctgaa accgacgata ttactttaac 108540
cctagatacc aatccattgt taatggatgc tttagctgct attaaggtat tatctgaaag 108600
aaataagtct cttgagacta aattacaaga gatgtctact gttattgaaa acattaacga 108660
aaagcttaat ttgatgacta gattatcaaa tatagaagca gagttagata aaatgaaagg 108720
tactagttaa tgtcacgtaa tttaatgcca aaaagtgggg ctatggcccc ctatgttgta 108780
gttaatagag acgccgcagt tgccggcgtt ttctctgttg atggagaagc tggtgctgtt 108840
gtgctaactt ccaaatacct gcaaatctct aagtatacta ctgataaagc tgctactgat 108900
gcagctatca ataatattaa cgagtcgatt ggtaatatta atactgcatt aggaggtgtt 108960
aatactactt tagatactaa agcttctaaa ggtgccaata acgatattac agaattaaat 109020
gcacttacta aagctattaa aatttctcaa ggtggtactg gggcagtaac gctagaaaat 109080
gctagagcaa atctacaagt agaacgccta cgtcagcaag atagcggaac ttttattacc 109140
tctcctgacg gtaaatattc tttatttatc tataatagtg gagattttgg tttaattaat 109200
agtgctagtg cagcagttag tgctatgaaa gttgctttcg gtggtacggg tggaactact 109260
cctaaaactg caagaaaagg ccttaatgtc cctgttggtg ccttggctga gatagtacct 109320
gatggagata acgtattgaa ctatgtagct gttgctggcc agagtgggta ttattcctct 109380
ggtgacgcaa tagttaacgg tcctcctaag caagagggtt ggtggacgta taacttccac 109440
tgtcatggag tagacataaa tggtgtggca caatatggta tacttcgtgc agttggattg 109500
tctggtagtt cctgggttaa cgttcttgat ggtactgata actggagagg atggcaggaa 109560
caatttaatg ttcaaactac tgttcaaatc tctaatgggg gtacaggtgc aacaaacata 109620
agccaagcaa gacgtaattt tggaattggt gaaactgata ttcctgtttt taggggtgtg 109680
aatcttattg aaaagaatgg tgctaattcc ggcattcttt acctaataaa caagaacgca 109740
gaaggtgtgc aactttcata ttccaggatc tacaatgaaa ttcaaggagc gaccgctaaa 109800
gcaacaatac aagtaacaag agaaggtggt gataataact attatcaatt tgacgagcat 109860
ggtaatgcac taaactataa ctctataaca gtaggtcgtg gtattggtaa cgctcttggt 109920
gataattcat tggttattgg tgatactgac actggcttta aatggggtgg tgatggtatt 109980
attcaaattc attgcaatgg tagtaatatt gcatcttatg aagcgcacaa tatgcatatt 110040
aatggattac ttacaatatg gcctactgag aataacgcaa atggggttag agtttcaggc 110100
gctagaactg gtggcggtaa tgcgttaatt gggggtcaaa ttgctggcgg cggctgggat 110160
ggttggcgag atcgcgcatc tggtctactt gttgaactac caggtgatga tgccgcatca 110220
aacatattca aggctgttag gtggggcggt gattgggctg ctggtcttga tgttgtaaga 110280
tatggtgctg gaggttgtga ggcgcatctt aatataagag gtgcaactta cacatttaat 110340
gatgctgggt atgcgtctgc tgttcagtgg gttaacacat ccgatattcg cctgaaagca 110400
aatctaaaag agattgaaag cgctaaagaa aaagtgaaat cgattaaggg ttatacttat 110460
tttaagcgta ataatcttga tgaagatgaa cactctattt attctgagga agcgggtgta 110520
atagctcagg atgtgcaggc tgttttgccg gaggctgttt ataaaatctc taacagtgag 110580
tatttaggtg taagttatag tggtattacc gctctcctag ttaacgcttt taatgaacta 110640
atgaagttgt tgaaaagcag cagcaagaaa ttgatgaact taagaaattg gtaaaacaat 110700
tacttgataa gtaaaagaaa accccagtgg atttccactg gggtttatta ttatctgctg 110760
ccagcaataa cacctaagtt aaacactgct aacatagctc taagagtagg atcttctgaa 110820
ttgctacata gttcgacaaa agcctcttca tcccactgga ttacattgcc ttcacgatct 110880
accttaagaa ctacgttatt cacatccggt tctgctacca taacatgatg atacgtagcg 110940
ggatgttcaa atgatcctat catattagga tcagcaaaat tatttacttc ctgagcttcc 111000
aaagccgcct tctccacgat cagtctcctc tagttcgtca acgatttcaa aatgatgagt 111060
tgagtagtgt ggtagtacta ctagctgaca aagtctctcg aaattctcca gagtttgcat 111120
ttcagaaccg tagttaaaca ggttcatctt aatagtacca cggtagtctg agtcgatcac 111180
tcctgcggta tttgcgatca ttagtttacg cttacctaaa gagctgcgag gaactaccaa 111240
accaaaccaa cctcgcggaa tttccaccgc gacaccggtg tcaatcataa gggatttgcc 111300
tggtgcaata gcacgtaaat ctgccgcaag gttagtacca aagaatgcac gcagatccat 111360
acctgcggca tcatcagaac caatcctcgg catgcaatct ggatgagtta gtttaatttt 111420
aatcattgtc ctgcgatctc caaaatatct ttcgtaaact tatctaatac gtcttgaccc 111480
acagcagcaa tagcatctac acaataggtc ggtaaatcaa ccagaatcag atttcggaaa 111540
agcaactcct ccgatgcgtt cagattctgt atatatttct gtttcccagg cagagggagt 111600
tgatcaataa tatccagaac attaccaaat tcacggataa tattataacc acgttttgca 111660
ccgatgcctt caacaccacg gatgttatca ccaagatctc ccataattgc tttcagagag 111720
ataaactgat ctacatcgtc aacgttatga tgttcataca tatcacgaag atggtactca 111780
cgacgtgtgg tgaaggagaa gcgagaaact cgatcagtta atagagtatc ccagtcacca 111840
tcagtagaga tcagccaaac gtgatcatat aaatgaccaa tgagcttaac aatataagct 111900
gccatatcgt ctgcttctac accacgaata gtgaaagttg ggaatgtggt ttcacacaac 111960
tcgaaagcat cacgaagata ctcgaagaac tgttcatcta gtgctttctc ttcttctgta 112020
cgttgagcat acttttcatc acggttgccc ttataatcag gaaggtgctc taaacggaat 112080
acagactttc ctttatcacc gagaacgatt gtagttctag ctgagtagga ttttgcgaga 112140
gattgaatag tagaaacata acttgaagcg aagggtttct tactattatt atgtttgaag 112200
cggaagccta agttagttcc atcaacaatc attaggttgc gacgagaagc cagctcagct 112260
tcttcctctt caataaattt tccccaggat ttactcatta tttaattaag tcctcaacag 112320
atgcgtgatg taaccacggt tcgaataacc cgattacgat ttccatgtct ttcttattta 112380
acaccatatg ggtacgactc attaagttgt cgaccatagg gtctgagcta tccaaagcaa 112440
ttaaccactg tcctctgtct ttcttgaata ttaaggctgg tttggagttc atctgttcac 112500
cttcacggga acactgctgc caccacttct ctagagtgga ttcaccaacg ttaaataaat 112560
tacttgatat gttatcatct ttataccact taacttcgaa gcaatattta ctaatgtgtc 112620
cgctttgtgg tggaaggtag atgtcaccct tcagtccatg gctctggcca aaagcaccag 112680
aacctggaac acgttcccac tcaagacctg tacgttcacg tagtatatct cttacctgat 112740
attcaccacg tttacctttc tctctactat ctacggccat gttttattct aagtaggaaa 112800
atccttctgc atcttttttg acagtaatct tatgggctaa tggatgcgta tgcccatgag 112860
aaacaatgat agaattcaga ctatcttcct catttaataa ttcaacgaga gtatcaagtc 112920
ctttcgtatc aataaagcta ataacttcgt caaggaacag aagattaata ttaactttac 112980
taatagatgt taacagcatt cgaatagcta acagagttgc taggttaatt ctactctgct 113040
gaccagtaga gcagttttcc atactggtgc ggtttccatc attgaagatt actacttgta 113100
atttagtttc atcaagttca aatccaagtg cgaacttacc accagtcata atagaaagat 113160
atttattaat caattcctca aatactttca cactatgctc tagtttatat cctaccagat 113220
ttttcaatgc agcgatcaga atatcaagat cagcaacagc ttctgatacc ccatccagtt 113280
tggacgttat ctcagacatt tctgcctcag ctttttcaat ctgttctagt ttcgctttat 113340
attttgcatt ggctaattcg acatttgcat tatgctcttt ggcaactgca actttagaac 113400
gtccatctgc gatttcctgt tctaattgtc ggatttgcac ctgtaggatt tgcacattga 113460
gttcttcaaa agaagcatca ctcattgaat ttttgaactc gtctctagct gctactgcct 113520
tatccaaagc atccttagct ctagtatatg cagtgtactc gacttgttct ttcttcaact 113580
gctctagtgt agcttcaaga gattgtttct ctttaaacag aggatcatac tctgttctag 113640
ccatatccat tgctttttga gcagcagttg tatcaaggtg agtaccacaa gtaggacatt 113700
cagtatttga agcctcttgc ttgaacttct gataacgttt cttgacttct cccgcacgtg 113760
aggtaacaat cgttaggtca cgcgtaacac ttgagatctc ttcagattgg tccgtgggcg 113820
cagggaaatt tttgaaaggc tcgaaagatt gttcggcaac ttgtacagct ttgtccaaat 113880
tacggagttt agtaatatta gcctcttgtg acttggctaa tgccgcctta attttcgatt 113940
cagtaagttc ttgtgctaac ggttcttcat caaattctgg aacttcaact ggctcttgta 114000
aacttccgag attgcctttt ccattgagga tcttcgtaat tacggccatc tggccttgca 114060
agttacttaa cgtacttgcc acttctttac gatcagcctt aatagtttct gacatctctt 114120
tatactgttc ctgattgaac agattaacaa gaaaagcttt acgtgtcgca tcagttgctt 114180
tgaggaagtc tagattggaa ccgacagatt gatagatcag tttagtgaat gtttgaaagt 114240
caccacccat aatctcttca atcatcttat acgtttgagt tgctgtgtgt ccactgatat 114300
cttctccatt cttaatcagt gtaaccttag cagttgattt aactacttta tgcaactcat 114360
actcatcttc atctttcgag aagtaagcgt gcatatcgta ctctttcttc ggagaacccc 114420
aagagaatag ggcgtccttc ttgataccac gtgagttctt gttatagaac aattcctcga 114480
taacagtagc aatagtggac ttccctagac cgtttcctcc gattaactga gtaactggat 114540
tcttatcgaa atgaattacg atatccttac cgtaagacat aacgttgctg aattttagtg 114600
tcttaattat aatctttgac atatttagca gctctagcca agattctatc aatgtcgtct 114660
tgagacagct tttcaacttc acggaagtaa agctcaagct cgcctaacat atcaagatca 114720
acaagattta acttagcatc tttagtaact cgatggttaa tctttttatc taagagatcg 114780
gaatctttaa tagactttaa ctgaacgacg tcaccagtga cttcataaat tacacggtca 114840
tagtcactag gctccatttc ctcaccaact ccgattgttt tacgaatcag ttgtggtaaa 114900
tcaccaagtt caatccattc tacttttaat gtgtcggtat caatgataaa acaaccattt 114960
gtaccttttg ttctttctct atggaaagat gtagttaatg gagatcctgg ataaagaagt 115020
ctagtagaac caacagtttg actattagta taagaatgta aatcgccagc aattacagtg 115080
tcataacagt tatacctcgt tagatcaatc tctggtttta catgtggagg gatttcacca 115140
cgaacatgcg tgaaacatag ttttgattca gatggtttcc actttggttt gtggatttca 115200
tcataaggga taatatcaaa ttcaggagaa cgatatggtt tagtaatgac ttcccaattt 115260
ccgtttgtta ctttattaat aactcctgca taatgataca gacatgaaat agttttagtt 115320
aacatttcat ggttcccagt gaagatcttg cctgaatggt caagtcttga catgaactgt 115380
tcaagaagtt ctatttcttc cgacgatggg tcggcaacat caagaatatc accaccagca 115440
atatgcagat cacagttatg attatggaat atgtcattta accgttctcc tagcatcagg 115500
aagcgacgct tctgccattc ctttggaact ttatcttgtc ctaatttgat atgatgatca 115560
gcactaaata gtattctcat aagttaaaaa gaaagggagc cgtagctccc cttggtttat 115620
tagtcgtcca gatcgctagc agcttcagcg tcgatgcctt tctgagaacc agcatcacca 115680
ccagacttag cgtcaccgcc gtcttgcttc tcacgacctt ccatgaaggc gagaatagct 115740
tctttctgct cttcgtagga cggaaccggg taggtttctt ccagagaagg aactttttca 115800
aacttgatga actcaccagt atcatcacac atagcttcac cgagaatatc ggcatctgcg 115860
gcgtgcagtt tagcttctgc gctagtagga tctttcagag ccatctggaa ctgcattgct 115920
gcgatctgct gtacatcgta ctcagtatcg aagcctttac ctttcttctc gatagaaata 115980
tcgatatcga acgggtcgga cagtttcagc tgcttcatga tagactggat gcctttcagg 116040
atagtagctt taacttccat aactttcagt ttgttatcag aacggtcgat aacgaaagcg 116100
atgtagtttt tcttcggttt cagcggaaca cgattaccat ctttgtccag ctctttctcg 116160
aagaagccca tttcgtgaat cgggtcagct ttaccgcgaa cgaagctctc tttagtacgg 116220
ttgaaacgca ggcactcaaa cggagctgca ttaccatctt tgttagtcag ccagtagaca 116280
taacgtggaa gaacaccaga aacgatacga acacgagtta caccgttgtt gaatttcagg 116340
a 116341
Claims (9)
- 살모넬라(Salmonella) 속 균주에 특이적인 사멸능을 갖는, 기탁번호 KCTC 13880BP인 신규 박테리오파지 KFS-SM.
- 제1항에 있어서, 상기 박테리오파지는 살모넬라 듀블린(S. Dublin), 엔테리티디스(S. enteritidis), 살모넬라 몬테비데오(S. Montevideo), 살모넬라 뉴포트(S. Newport), 살모넬라 파나마(S. Panama), 살모넬라 살라메(S. Salamae), 살모넬라 센프텐버그(S. Senftenberg), 살모넬라 타이피(S. Typhi) 및 살모넬라 타이피뮤리움(S. Typhimurium)으로 이루어진 군에서 선택된 1종 이상에 특이적인 사멸능을 갖는 것을 특징으로하는, 신규 박테리오파지 KFS-SM.
- 제1항에 있어서, 상기 박테리오파지는 pH 3.0 내지 pH 11.0의 범위에서 용균 활성을 갖는 것을 특징으로 하는, 신규 박테리오파지 KFS-SM.
- 제1항에 있어서, 상기 박테리오파지는 -10˚C 내지 60˚C 범위에서 용균활성을 갖는 것을 특징으로 하는, 신규 박테리오파지 KFS-SM.
- 제1항의 박테리오파지 KFS-SM을 포함하는, 항생용 조성물.
- 제1항의 박테리오파지 KFS-SM을 포함하는, 사료 첨가용 조성물.
- 제6항의 사료 첨가용 조성물을 포함하는, 사료.
- 제1항의 박테리오파지 KFS-SM을 포함하는, 소독제.
- 삭제
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| US20120088676A1 (en) * | 2007-12-28 | 2012-04-12 | Institut Pasteur | Molecular typing and subtyping of salmonella by identification of the variable nucleotide sequences of the crispr loci |
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| KR102526744B1 (ko) * | 2022-01-28 | 2023-04-28 | 국민대학교산학협력단 | 박테리오파지를 포함하는 코팅 조성물 및 이를 이용하여 제조된 항균 필름 |
| WO2023146038A1 (ko) * | 2022-01-28 | 2023-08-03 | 국민대학교산학협력단 | 박테리오파지를 포함하는 코팅 조성물 및 이를 이용하여 제조된 항균 필름 |
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