CN113322242B - 一株多重耐药性大肠杆菌噬菌体及其应用 - Google Patents
一株多重耐药性大肠杆菌噬菌体及其应用 Download PDFInfo
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Abstract
本发明公开了一株多重耐药性大肠杆菌噬菌体，保藏编号为CGMCC No.21992，对全基因进行测序后发现，不具有毒力基因和溶原性基因；通过相关实验验证，该噬菌体具有良好的耐强酸性，对温度也有一定的耐受性，同时具有较宽的裂解普和较高的裂解率，为工业化生产噬菌体用于禽类养殖环境中致病性大肠杆菌BE‑20199的防治提供了噬菌体来源。
Description
技术领域
本发明涉及生物技术领域，特别是涉及一株多重耐药性大肠杆菌噬菌体及其在禽类动物致病性大肠杆菌感染中的应用。
背景技术
禽大肠杆菌病是由某些致病性或条件致病性大肠杆菌引起，是危害养禽业最为严重的细菌性疾病之一，在疾病预防和治疗过程中最易产生耐药性，导致抗生素用量增加和禽类产品药物残留等一系列问题。该病一年四季均可发生，但以冬春季节和气温多变时期多发，给养禽业带来严重的经济损失。禽大肠杆菌病在临床上主要通过抗生素防治，但由于抗生素的盲目添加或过量使用，导致大肠杆菌的耐药菌株不断增多、耐药谱不断扩大，进而导致治疗效果下降，养殖效益降低，同时对食品安全构成威胁。目前，大肠杆菌病仍是危害畜禽养殖业的主要细菌性传染病之一。因此基于养殖效益、食品安全和公共卫生方面的考虑，寻找一种新的治疗大肠杆菌病疗效确实、无残留、符合国家减抗或无抗养殖发展方向的抗菌制剂乃当务之急。
细菌耐药性愈发严重，但是新型抗生素的研发速度远落后于细菌耐药性増长的速度，因此需要寻找新的替代抗生素杀菌物质来预防和治疗耐药性感染以进一步减少抗生素的使用。许多研究证实，噬菌体治疗是抗生素治疗的一种很好的替代方法。
噬菌体以其特异性强、与抗生素无交叉抗药性、无残留、对人畜无感染性、容易筛选获得等独特优势成为潜力巨大的选项。噬菌体制剂作为一种新兴的抗菌用品，因其不同于抗生素会引起菌群失调且可减少药物残留问题等诸多优点，越来越受到广泛的关注。
本发明是从养殖场污水中分离出一株裂解性强，噬菌谱宽的大肠杆菌噬菌体，初步了解其生物学特性，通全基因测序，未发现带有毒力基因和溶原性基因，从基因水平上证明其安全性，并对其生物学特性进行分析，以期为噬菌体防治大肠杆菌的研究提供理论依据。
发明内容
本发明的目的在于提供一株耐酸型大肠杆菌噬菌体RDP-EC-20155，旨在解决养殖场禽类因感染大肠杆菌，发病急而治疗不及时，且因致病菌耐药性而导致用药无效等问题。本发明的另一个目的在于提供大肠杆菌噬菌体RDP-EC-20155的应用。
为实现上述目的，本发明采用如下技术方案：
一株多重耐药性大肠杆菌噬菌体RDP-EC-20155，透过电镜观察，该大肠杆菌噬菌体RDP-EC-20155有一个多面体立体对称的头部，包裹着核酸，直径约68nm，有一个长约160nm的尾，有尾鞘，颈部连着头部和尾部，根据国际病毒分类学组织病毒分类第九次报告，该噬菌体归类为有尾病毒目肌尾病毒科；不具有毒力基因和溶原性基因，该噬菌体于2021年03月25日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为：北京市朝阳区北辰西路1号院3号，保藏编号CGMCC No.21992。
进一步地，该大肠杆菌噬菌体RDP-EC-20155经70℃处理60min，效价仍保持在108pfu/mL，经80℃处理60min，效价保持在106pfu/mL，对温度有较好的耐受性；在pH2-11的范围内，37℃培养1h，效价仍保持在106pfu/mL，在pH＝7时，效价最高，在pH＝2时，噬菌体仍保持较高的活性，该噬菌体能够耐受强酸和弱碱。
进一步地，该大肠杆菌噬菌体RDP-EC-20155与抗生素具有协同作用。
本发明的另一目的在于提供该大肠杆菌噬菌体RDP-EC-20155在制备预防和或治疗大肠杆菌引起的疾病的药物中的用途。
本发明还提供了一种用于防治大肠杆菌的杀菌组合物，包括有效量的大肠杆菌噬菌体RDP-EC-20155。
本发明还提供了一种清洁剂或消毒剂，包括大肠杆菌噬菌体RDP-EC-20155。
本发明的有益效果体现在：
(1)本发明从山东一养殖场发病白羽肉鸡的肝脏内分离得到了大肠杆菌BE-20199，经药敏检测和PCR检测，该病原菌BE-20199对多种抗生素均有耐药性，同时含有多个毒力基因，具有较强的致病力。大肠杆菌噬菌体RDP-EC-20155是从发病白羽肉鸡的肝脏组织中分离得到，通过相关实验验证，该噬菌体具有良好的耐强酸性，对温度也有一定的耐受性，同时具有较宽的裂解普和较高的裂解率，为工业化生产噬菌体用于禽类养殖环境中致病性大肠杆菌BE-20199的防治提供了噬菌体来源；
(2)该大肠杆菌噬菌体RDP-EC-20155经70℃处理60min，效价仍保持在108pfu/mL，经80℃处理60min，效价保持在106pfu/mL，对温度有较好的耐受性；在pH2-11的范围内，37℃培养1h，效价仍保持在106pfu/mL，在pH＝7时，效价最高，在pH＝2时，噬菌体仍保持较高的活性，该噬菌体能够耐受强酸和弱碱。
(3)本发明提供的噬菌体RDP-EC-20155和抗生素有协同作用，一方面噬菌体可以提高药物的敏感性，药物可以提高噬菌体的裂解性，这在动物疾病治疗过程中有非常重要的使用价值。
(4)本发明的大肠杆菌噬菌体RDP-EC-20155可应用于治疗或预防由大肠杆菌引起的感染性疾病。
附图说明
本发明上述的以及其他的特征、性质和优势将通过下面结合附图和实施例的描述而变的更加明显：
图1为本发明中宿主菌BE-20199耐药性试验示意图；
图2为本发明中噬菌体RDP-EC-20155的电镜图；
图3为本发明噬菌体RDP-EC-20155的一步生长曲线示意图；
图4为本发明噬菌体RDP-EC-20155的酸碱稳定性示意图；
图5为本发明噬菌体RDP-EC-20155的热稳定性示意图；
图6为本发明噬菌体RDP-EC-20155的遗传稳定性曲线示意图；
图7为本发明噬菌体RDP-EC-20155对宿主菌BE-20199的裂解曲线；
图8为本发明噬菌体RDP-EC-20155应用效果试验示意图；
具体实施方式
下面结合附图和实施例对本发明作进一步说明。以下实施例将有助于本领域的技术人员进一步理解本发明，但不以任何形式限制本发明。应当指出的是，对本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进。这些都属于本发明的保护范围。
本发明从山东省某养殖场发病白羽肉鸡的肝脏中分离得到大肠杆菌BE-20199，经药敏检测和PCR检测，该病原菌对多种抗生素均有耐药性，同时含有多个毒力基因，具有较强的致病力。大肠杆菌噬菌体RDP-EC-20155是从发病白羽肉鸡的肝脏组织中分离得到。通过相关实验验证，该噬菌体具有良好的耐强酸性，对温度也有一定的耐受性，同时具有较宽的裂解普和较高的裂解率。
实施例1病原菌及噬菌体的分离及鉴定
1、致病性大肠杆菌BE-20199的分离及保存
从即墨田横养殖鸡场采样，无菌操作取病鸡肝脏，在选择性培养基上划线，37℃培养18-24h后，病菌在LB培养基上形成乳白色、圆凸、表面湿润的光滑型菌落；在麦康凯琼脂培养基上形成表面光滑湿润的粉红色菌落；在LB肉汤中形成白色粘稠沉淀，振摇后呈均匀浑浊；挑取典型菌落继续划线纯化3～5次，直至菌落形态一致，然后挑取单菌落接种于5mL的LB肉汤培养基中，37℃，200rpm振荡培养12h，得到均匀浑浊的细菌悬浮液。再通过16sRNA分子鉴定和血清型鉴定，确定为致病性大肠杆菌，将其中一株命名为BE-20199，鉴定好的菌株在LB培养基平板上划线，培养12h，将全部菌落用接种环刮至30％甘油-LB液体中，保存于-80℃冰箱。
2、K-B纸片琼脂扩散法检测宿主菌BE-20199药物敏感试验
根据抗生素敏感性试验执行标准M100-S17 CLSI对临床常用抗生素庆大霉素、氨苄西林、头孢曲松钠、头孢唑啉、氯霉素、阿米卡星、卡那霉素、环丙沙星、诺氟沙星九种药物进行敏感性试验，以Escherichia coli ATCC 25922作为做抗性谱试验的质控菌株。具体方法如下：
(1)制备MHA培养基：将灭菌后的MHA培养基融化，冷却至50℃左右倒入平板，平板厚度约4mm，静置半小时，使培养基凝固。
(2)制备菌液：用移液器分别吸取10μL宿主BE-20199和质控菌株加入到5mL液体LB中，于37℃，200rpm振荡培养12h。
(3)取6-6.5mL加热融化的液体琼脂粉，加入到10mL灭菌后的EP管中，再吸取200μL的待测菌液加入到EP管中，颠倒充分摇匀之后，直接倾倒在MHA平板的培养基表面上，盖上盖子，水平放置在培养箱中，待菌液完全被培养基吸收即可。
(4)在MHA平板背面标记好放置抗生素纸片的位置，并标明抗生素名称。对超净工作台进行紫外消毒，用酒精灯外火焰灼烧摄子尖部，再用缓子夹取灭菌后的小纸片(圆形滤纸)平铺在MHA培养基表面，取1μL相应的抗生素加在每个小纸片上，静置，待抗生素完全浸入纸片即可(为了能准确的观察结果，要求纸片中也的间距≥24mm，可用镊子轻压纸片，保证其与培养基紧密贴合)。每组设置平行实验。
(5)将MHA平板倒置于37℃培养箱，培养12h左右。
(6)培养完成后，用游标卡尺测量抑菌圈直径，根据直径大小判断细菌对抗生素敏感程度，判断标准依据肠杆菌科细菌抑菌圈直径执行(见表1)。
表1肠杆菌科细菌抑菌圈直径判断标准
试验结果如图1所示，由图1可知，大肠杆菌BE-20199对临床常用的多种抗生素都有较强耐药作用，仅对庆大霉素、头孢曲松钠和阿米卡星敏感。
3、大肠杆菌噬菌体RDP-EC-20155的分离及鉴定
(1)病料处理：从养殖场取病鸡，解剖留取肝脏，无菌操作剪取2g肝脏组织，加入2mL灭菌LB液体，将肝脏充分研磨，然后10000rpm离心30min，取上清液过0.22μm滤器，将滤出液备用；
(2)制备噬菌体富集液：取0.2mL菌悬液和1mL滤出液加入5mL LB肉汤中，37℃，100rpm振荡培养过夜，然后10000rpm离心5min，取上清液过0.22μm滤器，滤出液备用；
(3)噬菌体分离：采用双层平板法进行噬菌体的分离，取噬菌体富集液滤出液0.1mL和宿主大肠杆菌菌悬液0.2mL混合均匀后，37℃水浴10min，然后铺双平板，放于37℃培养箱培养6-8h后，挑取透明斑于1mL SM缓冲液中4℃浸泡12h，取0.1mL浸出液和0.2mL菌悬液37℃水浴10min，铺双平板，37℃培养箱培养4-6h纯化，照此步骤纯化5次，即得。RDP-EC-20155在双平板形成的噬菌斑直径在1mm-3mm。
噬菌体RDP-EC-20155保存方法：将噬菌体增殖液与60％的甘油按照1：1的比例混合，保存于-80℃冰箱或液氮中均可。
取20μL含噬菌体粗制颗粒的液体滴于铜网上，自然沉淀15min，并用滤纸从侧面吸去多余液体，加一滴2％的磷钨酸(PTA)于铜网上对噬菌体染色10min，然后用滤纸从侧面吸去染色液，待样品干燥后用电子显微镜观察噬菌体形态，如图2所示。
由图2可知，该大肠杆菌噬菌体RDP-EC-20155有一个多面体立体对称的头部，包裹着核酸，直径约68nm，有一个长约160nm的尾，有尾鞘，颈部连着头部和尾部，根据国际病毒分类学组织病毒分类第九次报告，该噬菌体归类为有尾病毒目肌尾病毒科。
实施例2噬菌体最佳感染复数测定
按照感染复数为100、10、1、0.1、0.01、0.001的比例将噬菌体增殖液和宿主加入LB肉汤中，并且确保培养体系的总体积相同。于37℃200rpm振荡培养8h后，常温下12000r/min离心5min，取上清液铺双平板测定其效价。具体参见表2。
表2最佳感染复数的测定结果
由表2数据显示，当感染复数为0.1时，培养8h后，增殖液较为清澈，效价最高，说明RDP-EC-20155的最佳感染复数为0.1。
实施例3噬菌体一步生长曲线的绘制
取对数生长期的大肠杆菌悬液，按照最佳感染复数的比例接种大肠杆菌BE-20199和噬菌体RDP-EC-20155，在37℃的条件下，水浴10min，然后常温下12000r/min离心5min，弃上清液，除去未吸附在宿主上的游离噬菌体，如此用LB肉汤培养基洗涤两次。再将沉淀重悬于37℃的LB肉汤培养基中，迅速置于37℃200r/min的摇床中振荡培养并计时。前30min每隔5min取样计数，30-60min每隔20min计数，60-300min每隔30min计数，以时间为横坐标，噬菌体效价对数值为纵坐标，绘制生长曲线，得出噬菌体的潜伏期、裂解期，并计算出平均裂解量。平均裂解量＝暴发末期噬菌体滴度/感染初期宿主菌浓度。
由图3可以看出，噬菌体感染宿主菌后的60min内效价没有明显变化，表明其潜伏期约60min，感染后60-140min内噬菌体的效价明显上升，之后趋于稳定，表明噬菌体裂解期约为80min。通过计算，噬菌体裂解量约为101PFU/感染细胞，表明噬菌体具有较强的裂解和复制能力。
实施例4噬菌体pH稳定性的测定
制备105cfu/mL的指数期宿主菌液，将噬菌体液按最佳感染复数调整浓度。调整LB液体培养基的pH值分别为2、3、4、5、6、7、8、9、10、11、12，取噬菌体液加入到等体积的不同pH值下的LB液体培养基混匀，37℃培养箱静置2h后，取100μL混合液与等体积的菌液混合，通过双层板法测效价，每个pH值三个平行，绘制pH-效价曲线，确定最高点的pH范围。结果如图4所示。
由图4可以看出，噬菌体RDP-EC-20155在pH2.0-11.0范围能维持较好的活性，噬菌体能保持较高的效价，在pH为13.0时，噬菌体效价降低。随着pH值的升高，噬菌体的效价下降明显表明噬菌体RDP-EC-20155虽不能耐受强碱，但能够耐受强酸和弱碱。
实施例5噬菌体最适生长温度测定
制备105cfu/mL指数期宿主菌液，将噬菌体原液按照最佳感染复数稀释到适合浓度，取100μL与等体积菌液混合，倒双层平板，分别置于40℃、50℃、60℃、70℃、80℃和100℃下培养120min，30min后每隔10min取出噬菌体液体，冷却至室温，然后通过双层板法测噬菌体效价，每个温度三个平行，绘制时间-效价曲线，确定温度范围。
由图5可以看出噬菌体RDP-EC-20155在70℃以下能保持较好的活性，在80℃时，活性仍然可以保持60min以上，90℃环境中1h内仍然有一定的活性，说明该噬菌体能够耐受一定的高温，具有较好的温度耐受性。
实施例6：噬菌体遗传稳定性实验
将RDP-EC-20155连续传代29次，每代培养基中加入等量的RDP-EC-20155和BE-20199，每代培养时间6h，然后倒双平板，测定其效价。
由图6可以看出，噬菌体RDP-EC-20155在传代29次的过程中，始终保持较高的效价，没有出现明显波动，说明该噬菌体可以稳定遗传。
实施例7噬菌体对宿主裂解曲线
在细菌悬液中加入噬菌体，由于噬菌体对宿主的裂解作用，导致OD600的值产生变化，由此得到噬菌体对宿主的裂解曲线。将培养6h的宿主菌悬液以1:100的比例接种到100mL LB培养基中，以最佳感染复数，向其中加入噬菌体增殖液，同时以不加噬菌体的作为对照，每组设置3个平行，结果取其平均值。
由图7可以看出，没有加入噬菌体的宿主，随着宿主的增殖，其OD600在40min内逐渐增加，60min-120min内呈指数增加，在180min后趋于稳定，而加入噬菌体的宿主，由于噬菌体对宿主的吸附、侵染，OD600增加比较缓慢，100min内其OD600没有明显的提升，但随着时间的延长，噬菌体裂解率达到顶峰，未被裂解的宿主菌继续繁殖，其OD600逐渐增大。
实施例8噬菌体裂解谱测定
噬菌体宿主谱采用点滴法检测。于细菌库中选取30株经鉴定为致病性大肠杆菌的菌株，各取100μL待测菌液分别涂布于LB固体培养基上，待晾干后，吸取10μL噬菌体液各滴2滴于培养基上，37℃倒置培养6～8h，观察是否出现空斑，若出现则说明噬菌体对该菌株有裂解作用。结果如表3所示。
表3：噬菌体RDP-EC-20155裂解谱
由表3可知，噬菌体RDP-EC-20155可对试验中所选的30株大肠杆菌中的24株有裂解作用，裂解率80％，说明该噬菌体有较宽的裂解谱。
实施例9噬菌体在治疗中的应用
实验方法：
(1)取14日龄左右SPF鸡40只，平均分成4组，每组10只，分别为空白组A，噬菌体组B，抗生素组C，生理盐水对照组D。
(2)每组实验鸡腿部肌肉注射BE-20199菌液，每只200μL(109CFU/mL)；
(3)120min后，B组口服噬菌体RDP-EC-20155(1010PFU/mL)500μL/只，C组口服头孢曲松钠兽药，D组口服生理盐水做对照；
(4)每隔2h观察记录各治疗组实验鸡的生理状态及存活情况；健康无异常记5分；出现轻度嗜睡伴有羽毛杂乱4分；出现严重嗜睡伴有羽毛杂乱和轻度瘸腿3分；出现严重嗜睡伴有羽毛杂乱、腿脚、喙破口流血记2分；濒临死亡状态记1分；死亡记0分。
由图8可以看出，实验鸡感染宿主后，2h内健康状况迅速下降，2h后对其进行治疗，结果噬菌体组和抗生素组均在8h后开始恢复健康，24h后噬菌体组和抗生素组实验鸡全部恢复健康，对照组则全部死亡，说明噬菌体RDP-EC-20155对鸡感染大肠杆菌具有很好的治疗效果。
应当指出的是，具体实施方式只是本发明比较由代表性的例子，显然本发明的技术方案不限于上述实施例，还可以有很多变形。本领域的普通技术人员，以本发明所明确公开的或根据文件的书面描述毫无异议的得到的，均应认为是本专利所要保护的范围。
序列表
<110> 瑞科盟（青岛）生物工程有限公司
<120> 一株多重耐药性大肠杆菌噬菌体及其应用
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 73310
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 1
cgttctctga atccttgcag aattcctcaa tacttttctt acgcagagtc tcagtcatag 60
ctgtttcctc tgccagttta ttaacaaagg tatgcatttc taatttagta cgaatggctt 120
gtttctctgc catcgtacta acatatgaat taagcatacc tactgcttta gttgctgtac 180
cgaaggtatc tgcaacagta gtagcagctg agttaacagt accaagtaaa gttcctgcag 240
tcatacgtac agtagcttga gtagtcatga tgatagctcc aatagttgat gataggacga 300
catgtccata acatgcggag catcgctgac gaacgaagtg agtcaagcat gtgtaacgtg 360
taaggtgggg tagggtagtg gctacaccgt caacataccg ggggggatgg ttgagtgtaa 420
gggtgtagcc ccagccacac cactatggct gtacatagct atcacaattt tgacatgcaa 480
aaatattata ggcactatca ctataagcag ataactttga ggtactaaca tgaaagatgg 540
atggggtaac tacaatccta cagaccctga gaactggcta cactttattg catgtgcttg 600
tgctgtagga ttcataggta ttgtgctctt atcactggtt ctatcttacc tgtaaatgtt 660
aagaggggta tctatctacc ccttattcgt catagcgttt gatacttaaa acttcataca 720
gcttaccatt tacaagcaat ggtccatcca cacctacgaa gactaggctg tgtactatca 780
ctggttcagg taagcccctg actgttacat accacatatt aatccttata agcagctaaa 840
taggaggaag gaatctggga gaagatatgg tcatagtaat agcaatatgc ttcactattg 900
gcagagttta taggcatacc tatgtagtca aacacccata aacagaaatg attgagttcg 960
tggatgagtg tatgtaaagg ttccttgaag ataccaatca atacacgatt gccagagtaa 1020
gaagtatatc ccttacaacc atccaagtct ggctcatacc ctttaagttt cttgtacttt 1080
ttaatgaagg tattctggtc gaatactact tctaactgca ccggataggg tgatgtgttt 1140
agtttcattg tctttcctta tagattgggg tgttatcctc gaaggatagg atgtacttgt 1200
ctgtattgag atgccatgga ctgaatctac cctgccagga cattggtgta cctggttcaa 1260
gtagatgtac ttctgctttc ttattgtgta agaaaagcaa tccttgtagt tcatgcaagg 1320
actgttcgtc tggaaccagg ataagtatat cagcatctag gttagctact tttttatggt 1380
gggtgtattc atcccaaggt atttctgaat aaattttact gataaccatt agcagctctc 1440
gtcgctagat aaatatgagg tagaaggata gagggtaata agtctacctg taagggtagt 1500
aactaccata ggttcactgg aggaatagat atacacaact acattctctg gaacttcaat 1560
ggaggtatct attttaataa aggtaatagg tacacccaac atgaggcagg ttccgatgaa 1620
ctcatctgct ttttcgcatt gttcgtagta catggtcata ggcgtgctta ttctaatcca 1680
gtgagattta cctaccccca gattaagggg actggtacta cgataactca aaccatagaa 1740
gcgcatacct tacttcctta catagtagta acgggattca ccatcctcat agcgacaatc 1800
ccattggtaa tcatcagaca ccatcatgta tgggataggt tccccggagt taaagttggt 1860
atagacagat ggaggaacaa tacatacaac cttacggtca ttacctgcta agaagagttg 1920
accccttacc tcatggtagt cctctaaagt aggaaccagg attacttgct gggggttgct 1980
gtccatgact tcttctaaag tgaagtctcc tgcatacata gccccccttc cagtaaaact 2040
agggatggtc attatgaatt tcatgattgg ttctccttcc tttagggata ggtctgaagt 2100
aaatggaaag tgttttatta aaaacattat ggtcagctat caattcccaa ccatctatct 2160
tatagaaacg tacaccaggg aagatcattt gaaagtcgct gtaaaaggaa agatcataca 2220
cctttggttc agtaagtatc ttagtattga gttgcaggtt ggataggaaa agagaaccaa 2280
taacctcatg ggactcttcg cagtcaggaa agaatactgt ttgccacggg gtaatgaact 2340
tacttactac ttctggttcc ccaaggtaat aaggttcctg tttaagtagt actccggtaa 2400
tatcaatatc aaaacagtag atgctcatat ttgcatacct cctgaaaaag aaatggggta 2460
gagcctctca actctacccc ttaccgtttt ctatcacggg tcagttcatc accctagata 2520
catgtgtatc gttagcctac taggtaggaa aggagccggg caaccctgac tgtagaagtt 2580
attaactctt tacgagtacc gccactgtct actaggcggc atgactaatc acatcgtcac 2640
cgtcatccga acactgtctt tgcttcgcct gacggagaca ctatgaggta attttgtgaa 2700
gaacacaaga gctgataatc gtttttctaa agagtaagtt tctcttggtt ctaagatttt 2760
aatgcctggg cgattctcta aaagcatcat cccagctaat tcatggtagg tatcttcatc 2820
atgtgtaatg ataatatcat ctgaaagaat accggcttta gtgtggtcta gctctttgta 2880
ttcatcttta gagtcgtgga ctctcatata gattctcaaa ggttactcct tttgggaata 2940
gggtcatcgg aacattacat actatttagt atcatgaact ttttctatat catgactaaa 3000
gtgtaactgt atggtatcgt ttcttcaagg ggtaactcac cccaagaaaa gaatcttctt 3060
taatagacat aactttgtag cgagataagt atgagtgaac taactaagca gcagattgtt 3120
aatgccttac ctgctaactt caaaaacagt gtgacccagg agttggtaga cacgatcaac 3180
aacataaccc aagaccaact ggttgctgag tccttccgtg aaaactttat ctcctactct 3240
ggtgtaatga aggaaggtaa gtttaagaca caggactaca tgaatgcagt tcagtatgtg 3300
acttataaac acatgggtta ttccaataag gaagcctact tcaagacatt ccctaaccga 3360
caagcagaac ttgttgcacg aggaaccagc gagaaagata ttagtgctta tgtatctgca 3420
tatcacagag gtaagttagt taacctcatc atggaacaaa gtttggttcc agtgtggatt 3480
gtgaaccagg acaactatca gaaggcaatc aacgttcagg tcgaactgat gactacagca 3540
gcaagtgaga aggtaagatg tgatgcagct aactctatcc ttactcactt agctaaaccg 3600
aaagatcagg taacgaatat caatctggac ttgagagaga actccggatt aactgacctc 3660
aaggcaacac ttgctgcatt ggctgaacag caaattaatg caatcaacag tggtgtacct 3720
accaaagagg ttgctggttc taaactagta cgtgcagatg aggacattac agatgtctga 3780
gttaattaaa caggaactag atgaatggct ggaccaggtg gattactccc accttaattc 3840
atctaactat gttcctagtc agtttgccct tacctttgcc aactttatta agttggttaa 3900
tggcaaagag ggtgaatcca acaaaacacc cccagttcac ttgaagatgc tggataagat 3960
gattagcccg aaccagtatg tagttaatct gtgcttccgt ggtgcaggta agactgctgt 4020
gttcatggaa tactttacat tgttcttggc agtattcggt cacttaccaa gtctgggtaa 4080
agtagaagga atgatttacg tatctgactc aatggataac ggtgctaagt ctgcacgtaa 4140
gaacattgag tttcgttaca agaatagtga gtttcttcag caatggatac ctgaagctac 4200
ctttacggat aacctgttag agtttcgtaa cgtagaaggg catcgacttg gcgttaagtt 4260
gtttggtgct aagacaggtc ttcgtggtac gaagatatat ggtaaacgtc caacactctg 4320
tgtactggat gacctgatta gtgatggtga tgctaactct aagacaagca tggaagctat 4380
taaagatacc gtctataaag gtgtgaacca tgctcttgac ccaacccgtc gtaaagtaat 4440
cttcaacggt actcctttta ataaggaaga tattatcatt gaagctgttg agtctggtgc 4500
atgggatgtc aacgtatggc ctgtatgcga gaagttcccc tgcagtaggg aagaatttca 4560
gggtgcatgg gatgaccgtt tctcttacga ctacattaaa gcccaatatg atatggcagt 4620
taagactggt aagctggcag gtttctatca ggaacttatg ctccgtatta gctctgaaga 4680
tgagagactg gttcaggata gtgaaattag atggtatagc agacagcaac tattaaggat 4740
gaagtcctgc tacaactatt acattacaac tgactttgct acttcggaga aacagaccag 4800
tgactacagt gtcatatcag tttgggctta tagttctaat ggagattggt tctgggttga 4860
tggtgtggct aatcgtcagc ttatggataa aaactttgat gacctgttcc gtctggttca 4920
agaataccaa ccgcaaaacg ttggggtcga aatcacaggg cagcaaggtg gattcatttc 4980
actccttcag aaagaaatga ttaaccgtaa tgtcttcttt aactttgcat cctccgaggg 5040
tggtaagcca ggcatccgtc ctgtaacttc taagctgtca cgctttaacc tggtagttcc 5100
atggttcaaa gctggtaaga tgtacttccc ggaagagttg agagattcaa ctattatggg 5160
tctgttcatg ggccagatta aactagctac cattaatgga atcaaaggta aggatgactg 5220
tattgatact atctccatgc tgggttatct caatccttgg aaaccacaag caggtatgac 5280
tttggtggat aacaagggtg acccgatgtg ggatgatgaa gaagaagata gtgttaaccc 5340
actacgttca tacattgtgt gaggaaccat gcagaaatta tcggaagtat atcgtggctt 5400
ggcactaagt accttgaaag gtactggtgc agtcactgat gacagattag atattgaacc 5460
agatagcaag cctgagatac tggctgctat taatgaaggg ctggtacgac tacatagtcg 5520
tttccctctt agaactaata gctgcattgt ggagatgaaa gaaggacgta ccgattatcc 5580
tcttcaatcc cgatatgctt actcccggtt tactaaacct actcttgagg ttcagtatcc 5640
atacatcatg gatgggttca tgaaaccttt ccaggatgat gttattaaaa tcctaaatgt 5700
gtttgataac tctgggaatc gacgtagact gaacgatgac tcagaccctc gtgctatctt 5760
caccccaaga cctgatacta ttcagtgtat gcgacctcga cacttcgagg cacttaacgt 5820
aacttatcaa gctaagcacc cagtacttac gggtgatgaa gaacaggaag ttgacttagc 5880
agatacgcta atgacagcac ttcataactg ggtaggttat cgttaccaca ctggtttgaa 5940
tactcctgaa gctaacgcta aggcagcaga gtatctgcaa acatatgaaa gcatctgtgg 6000
tgaggttgtt gattacgacc tggctaatgg aagtatttca attactgaaa ctaaatttga 6060
acaacgagga tggcgatgaa cgcacatacc ccctttgatg caaaacagga ttggaccaat 6120
ccttactgcc agaacagttc caatgaccca atggtagatg cactacttgg taatgcttac 6180
catgtggttc gaactgttta ctgcaatcta ggtaacctta aactcattta tgatttcttg 6240
aataaatatg gaatggtgtt aggtgttcaa tcagaaactg aactgaaggc catgcctacc 6300
agtgcatctt atgtacgact gtatggtttt gataatactc ttaaaagagt tgttacagac 6360
tatctgtatg tagagggtga ccgtacaggt gtaattccgg atgacccttc agctactggt 6420
tcctggattc tggttgccac ttctaattca gacagtggtg gagatgatgg tgaaggtaaa 6480
gcatcaccac catacattcc atactcatat aataatggtt ctgctatcgg tggtgaaact 6540
actatcccag tacctgctgg tacggttggt gtaccgatga ttgtaattgg tggctatacc 6600
aacttggttg gttatggttt tacttatgat gcatctacac taactgttac tctggctcaa 6660
ccattggaac caggtgatga agtgcacctg ttcctgactg gtactccagc agttcctaat 6720
aatcctaatg tgtctgactg ggttcagatc aactggctgt acaacggtgg atatgcttca 6780
ggtggcgagc aagtaattgc tattccttac acatttgaat cagtatcggc tatctataag 6840
aatggcgaac gttactatgc aggtttagca gataagtctt acgcagttga tgctgctaac 6900
caacgtatcc tattgactga gccattagct accaatgacc gtctgattgt tcagattggt 6960
ggtgaatcca ctactctcat tatgtctgac cgtactgttc aggaagtagc tcgttctgct 7020
aacgtacatg agaatgatgt cattctgagt acaaatacca ctcagtattt gaatggtatg 7080
aaggtagtat atgatgtggt agggcagaag atttatggtc ttcccactct acctgctaac 7140
gtttacatca attctgtatc caacggtcag ttaacttact caccagggaa tatcactgta 7200
gacctggttc ctactccgga ttcctatgag aatctggctg cacctaaggg tgctggcgag 7260
attgggtacg acccagcaga attatatgat gatggaactg ttgggggaat gctggaagac 7320
ctcactgacc gtaccttgtc tatggatgac ttttctggct cagatgtggg cattaagatt 7380
aacgctggat tagattttct ccgttcgcag aacaccacct ataaagtggg aggacgtctc 7440
aatctaccac gtagacaaat gtcgctagat accccaatag tgtttgaccg tgttccagca 7500
ggtgaagatg atacaggaga tgaatactta atctctggtt ctggcaaggg tggcactctt 7560
cttgttgctg cttctggttt tccggcagga agtgcaatct taaatactaa tctaccaagt 7620
ggtaaacatg ttcaaggctt caagttgaag gacttaggaa ccaaaggtgg atacaacggg 7680
ttgcgtgttg aaactgcatc tcgtacaacc attgataacc ttagggtatc cactgctaca 7740
tcggatggtg tgtatatcgg taatagttgg gttaacgtct ataaccaggt tcttgctgac 7800
caatgtactg gcaatggtgt taacttctca gtatcagcac agaaaaccag taccaccacc 7860
cttgcagggt atcaattgaa gaatggcggc aatggttggc gatttgggtt tatgaactac 7920
tctgctgcca ttgctccagc atctgaccag aatgctggac atgggtatgc tattgccaag 7980
tcggaaggtt ttgttatggt tgctcctggt gctgagagta ataccggtgc cggtattatg 8040
gttgaagcat caagcagcct gggagaaaac agaagtattg ttattcatgg cgccttttgc 8100
cacaacaatg gttcaggtac tcatgcaaat ctgcttcatg caattagtgc acagaatacc 8160
atcaaccgtg ttcgaatagc ggatagtaca gcccacgcta gtattggtac aaccccagat 8220
attattgctg atggcaatga aactgtggtt attgtagata actgtgttct gcctaatggt 8280
tggcaatcac gtaatggtgg ttacattgat tgggtacacc atactttata cacccagaag 8340
tctggtgtat cagggccggt agcagtatgt aacctgagaa gtacccaagg acatggtaat 8400
aactctgaat ctagtactga gtcgtttgcc ggtgaagtaa ctatagtcgc tagtgttaac 8460
ccaccttcta cagctacacg tcgtattgct atttataaac tgctagtctg ttctggtgca 8520
gatacaggta aagagtgtga gttaattaag cagcttggtt acgtgtcggg taacttacca 8580
ggaacaccta gctttacctg gtcaatcggt tcatccaacg aactcattgc tacgccgatt 8640
ggttctgctg cgggtacatt ctacttcgaa attactactg attctcaggt agtagcgctt 8700
aagcgttaaa caaattcacc cctcttcgga ggggtttcct agaaaaacaa tgaggtactt 8760
atgaacgaaa tgttctcgca aggtggtaaa ggctctactg gtatccttac caataagcaa 8820
gccattgccc gtaagtttgg tgttaagcag aatgaagtag tctactttgc tgtgggagta 8880
gacttgagtg gatataaagt catctatgac aaggagaccc aaagagctta ctcactacct 8940
gctgatattg ctcctggtac tactgccatt agtcttagta ctgctgctgt acttgtgcat 9000
tccgctggct ctgtagatct cggggaattg gctgtatctc gtgaagagta cgtcacattg 9060
cctgggtcgt ttgacactgg tgctacactt aatgttaaaa atgaattact tacccataca 9120
acaggtaagt atcgttggga tggggagttt cctaagattg ttccggctgg gtccactcca 9180
gaaaataccg gcggtataac agcttccagc tggttaccaa tccgtgatat cactttacgc 9240
tcagaacttg cgtctcacga cacaggaaaa ggtgacgccc ttgtcggagt gaagcagcct 9300
tttactggcg ctgtgggtat gacacaacat gataaaaata aacagttggt gcatgttgca 9360
gacttcgggg cagtcgccga tggtgctact gatgcaagtc ctggtatcaa cgcagcagtg 9420
gcgtggctga aggccagtgg tggtggtacg ctttatttcg gtgtaggaac atacttgtgt 9480
aactctgtta tagatttgaa gggtgcgtat gtctgtttac aggggtccgg catgaccagc 9540
actactatta aggcgggttt ttctggagat gccctgattg atttatatga aacgtctgat 9600
gtacgtatag ctcctatcac cattagcgat atggttatta gcggtaactc taccgtagcc 9660
cgcacagtct ccctacgata caggcactac accaagtttc agaacgtaat tttcgagggt 9720
ggtggcagcg cggctttcta cgctaaggac gcttggctaa acgcttttga caattgtggt 9780
tttgagtctt ctccatttgg ttgccacctg gacgggtcta accaccgtac acgcatggat 9840
agctgttctt tccagggttg tacaacaagt tgccttgcag tacgcagtaa cggaaccgcc 9900
aacgacggta acagtgcatt aatgtttaat aactgcgact tcgagttttc cagttctgct 9960
ggtgtcgatt tctcgggcac agacgcaacg ttcaattgct gttgcgtagg ggaaaatctt 10020
actaactcgg tattcctcgt gtatgagggt aacatacgcg ttaatggggg cgttatggct 10080
ttcggatata ccgcaaacac atacctagcg tacctcaacg gcggtactat aatatttcag 10140
ggttcggtaa taaacggtca gacacacgcg gccataggta ctcttggcgt gtcatctggc 10200
ggtaaaatgg cgttgaagga gtgcactaca aacataccta caggtcagtc tatgatggcg 10260
ggtgatgttc ttttaagcgg catatgctca cataaggttt tcgcgccaag actcggagta 10320
gattatgcca gctacactaa caacaccacg ataactgata ccccatctgg taaccaaaga 10380
acagttacct tggtaaccgc ccctgggcca tctcctgtgt ttgggctacg tgctacactt 10440
acggatatgc agtggcgcga tggggagaag tggtatgtca ttgttaccta ctccagtaac 10500
gtagacttta ttctccgtgt tgcagcaacg ccactaggcg aaggtacgat tattggtaca 10560
ctaccatcca ccggcggagc agtgcgaacc gtagttttag cgtcagtcgc agcggtaaga 10620
acaaccgcaa cagttattga gatatacagg gatagttcca tgtccacagg agatacgttt 10680
actctgcgag acatatcttt tggagacagt agagccttag gtgcagattt tggcggcaat 10740
tacggaaata tgtataaatt ctaaaaataa ggccccagat ggggcctttc tttatataga 10800
gtttactgtt tgcgttcccc attctcttgc tgtaccggca cttcttacaa taattcctag 10860
gacagacact atgtctggat gtacttcatc atactaagta ttatctaaga gtcatcttat 10920
gatatgaccc tattggggtt cctaactata caactacggt aatattatga atgaatcggg 10980
tgaactgctc ctagtcagac tggaggaacg attgaagact ctgtttgaga accagacaag 11040
agacagacag gaccgtgaga aactggagga aactctggta gaactacggg attcaatcaa 11100
tgacataggc aatagactaa agaatgtgga ggatagctta gctaagaatg aacctactat 11160
tgaagagttc atcaccatta agcataaagt tgttggtgct ggaattttcg gtaagtgggt 11220
atgggctggg gcaggtgcaa ttatcggtgt gttggctgct gcaagacggg agatattcgc 11280
atggttcgca ggctaaaaag aaaggtagag ctagtaccta actggaaaag atgctggaag 11340
tgggcatcca tccagattag tacgctcggg ttaattatgt tttctgccat agatatcatt 11400
caaccaatgc tgacaggatt acctcgtcac atcctggaag atattcccca tggctccaat 11460
attgcaatcg cactgtttgc cttaaatatc gtaggcagat taatacgatt ccgaccaaag 11520
gaagattcac atgaaagcta aacagaaact tgctgcgaaa ggtggtgcag tgggaggagt 11580
tgtcgctgct atcctggggg cagtgtttta tatggaaggg ggttatgtaa ataatcctcg 11640
tgacccaggt ggggaaacta atcatggaat tacccaatct gtagctaaat cccataaaga 11700
agttctggct actgagtacg gctgggatgg ttctatgaag ggtttatcta aagagatggc 11760
tgctgagata tacattgatg actacgtact taagcctaac ttcgtagcct tcgctgatat 11820
atctccagct gtaacacata agctggtaga tgctggtgta aacactggtg taacccgtcc 11880
agctaagtgg ttacagttat cccttaatga aatgtcccgt gatggcaaag actaccctaa 11940
aatacaggtt gatggtaaag tcggtacaag tactgttaat gcatataaag cattgcagaa 12000
aaaacgaggc aaagttgcag catgtcaggt aatgattaag ctactagatg ccaaacaact 12060
gaaccactac acatcattaa acatgccaga ctttacgtat ggttgggtag ctaaccgaat 12120
aggcaatgtg ccactggagg catgtaatga agatgctaat ctctaaaggt tggccttatc 12180
ttctggtggt ggtccttggg gccaccattt acttttgggg taattccaat gggcagtcta 12240
ctgttcaaaa gaagtgggat gaccaaaagg ttgaagatca aagggcaata caaaaattac 12300
aggacaaata caatgctctc caaagaaacc atagttatga agtcgggtta ctcacttccc 12360
ggttgcagac ggctgagagt aattacgcaa gcgagcttgc tcgcgttagc agtgattacg 12420
acagccggat gcaacagtct gaaagacgag catcgatata taaacgtcaa gccgaagccg 12480
gaacctttga gtgcagaagt cttgcaagcc atgcagccag actcgacaac agtcttgaag 12540
aaggcagacg tttggttgaa gaactcaggg caactgttag actccgtgac agccaactaa 12600
ttgagttggg taaacagatt caggctgacc gtaaactttt tgaacaggaa taactatgga 12660
acaccaagac acttttaagc ctttaccaga cccggctcaa tcagagaagt taacggactg 12720
gaagaaggaa ccaagtatcc aattactgaa gggtgatttg gaatcagcta aacctgctca 12780
cgatgcaatc atggctcaga ttcgtgagtg gaatgacctg atggaagtta agggtaaggc 12840
taaacctcca aaggttaaag gacgttcaca agtccaacct agactggttc gccgtcaggc 12900
agagtggcgt tatgcaccat tatctgaacc attcctttca tccaataaac tctttaaggt 12960
taccccagtt acttttgaag atgagttagc tgcccgtcag aatgaactag tgcttaacta 13020
ccaattccgt acccagctta ataaagtaaa actggtggat gattacgtac atagtgtcgt 13080
agatgatggt actggtattg ctcgtattgg ttgggaacgt aagactgtta agattaaaac 13140
agaaacacca gtctttcagt tgtatccaat tgagaaccag gaacaagcag atattcttca 13200
gcaggctcta cagcttcagg cagagaatcc tcgtgagtat gacgaaacaa tgccagaaga 13260
tattaaggaa gctgttaact acttcaatga aactggggaa gctacctatg caatccagac 13320
tggtgttact gaagtagagg tagagaaggc actggttaac agacctactg ttgaaatgct 13380
taaccctaat aacgtagtta ttgacccgag ttgtaatggt gatttagata aggctctgta 13440
tgcagttatc tcattcgaga catgcaaagc agaccttatg aaaaacaagg atcgttatca 13500
caacctggat aagattgact gggaaagttc tagccctatg accgacccag accatgaaag 13560
taaaactcct agtgacttcc agtttaaaga tgcacttcgt aagaaagtag ttgcttatga 13620
atactgggga ttctacgaca ttaacgatga tggttcactg gaaccaattg ttgctacctg 13680
gattggttct actcttattc gtatggaaaa gaacccattc ccggatggta agttgccttt 13740
ggtagttgtt ccttatatgc cacgtaaacg tgaactcttc ggtgaagctg atgctgaact 13800
gttgggggat aaccaagcaa ttcttggtgc aaccatgcgt gggatgattg acctgttggg 13860
tcgaagtgca aacgggcaac gtggttatcc taaagggatg ttagatactc taaaccgtcg 13920
tcgatatgat gatgggcaag actatgaata caacccaatg cagggtaatc ctagtcaggc 13980
cattatggaa cacaagttcc ctgagttacc tcagtcagca atagtgatga ctcagatgca 14040
gaaccaggaa gcagaggcac taactggtgt taaagcattt tctggtggtg taactggtgc 14100
agcttatgga gatgtggctg ctggtattcg tggtgcactc gatgcagcat ccaaacgtga 14160
gatggcaatc cttcgtcgat tagctaaggg tatggcagac attggtacta agatttgtgc 14220
aatgaatgcc gtattcttat ctgagaagga agtagtacgt attaccaatg aacagtacgt 14280
agaaattaac cgtgaagacc ttaaaggtaa tttcgatatt gaggttgata ttaatactgc 14340
tgaaattgat aaccagaaat ctcaggactt gagcttcatg gttcaaacgt taggtaatac 14400
agttgaccag actattacat tatctctcgt agctaaaatt gcagaactta agcgtatgcc 14460
agaccttgct catgaattac gtacctggcg accacaacca gaccctatgg aagaacagct 14520
taagcaattg gcaattcaaa aagcacagct tgagaatgaa gagttgaaaa gtaagattgc 14580
actcaataat gctaaggcta aagaagctat ttctagtggt gaccttaaag acctggatta 14640
tctggaacag gaatctggta ctaaacatgc tcgtgatatg gaaaaacaaa aagcacaatc 14700
tcaaggaaac cagaaccttc aaataactaa ggcgttgaca acacctacta aagagggtga 14760
aactaccccc aacatatctg ctgctgtcgg atataacgct ttgactaacg ggaactcttt 14820
acaggaacgt gacttagctg cacagcaaga cccgacttat tcacttagtt cccaacatta 14880
tgaccctagc caagacccag catcggctct tggtatgaac ttataaggca gaccttcaca 14940
tgagtaaaga atcaacaatc gcaggactgg aacatcgact ggaagctgct aaaggtgcag 15000
ttgcacacgc agaggatgta cgtaaacttc tggagaatcc actgttccgt aaagtaatcc 15060
ttcgacagtt ctgtgtagaa gattgtgcac gttacgtgca ggagtctggt gacccactgc 15120
ttgaagctaa taaccgtgca gatgcactta acatggctca agctgctggt cacttacgtc 15180
gttggctcga cctcgccatt cgtatgggtg aaaatgaagc tgacgttatt cttcaggtag 15240
aagaagaact cgatgcagtt cgtgctgaac ccgaagacga ataattaacc ggagatattc 15300
cctatgacta cggaagctgc aaccacagct tccgacattc tcgcaatgtc ggatgatgag 15360
attctgaata tggaagcccc agctattatt gctgaggaag acacttccac tcagaacaac 15420
ccagaaacca acggcgtaca gactcctgat gaagaagttg acccctctgc tgttgaagac 15480
ctaccaaccg aagaaacctc ccctgaaact gaaccagttg aggatgattc agcaaattct 15540
ttaacttctg ataaagttga tgataaagtt gttgacacag aagttgatag taacggtaag 15600
ccgattacta aagctgaacc ttctactgct gaaccaggcc aggaacagaa agaagaaggc 15660
aaacagtctg aaggtctgcc tgctgacttc aactataagg aaggttatga gaagttgatg 15720
gctcctttta aagctaacgg caaaatgatt actccccgtt caccggagga ggccattagc 15780
ctgatgcaga tgggtgctaa ctatactcgt aagatgcaag aacttcagcc gtaccgtaag 15840
gtaatgctga tgttacagaa caacggttta atggatgagg aaaaactttc tttccttatt 15900
gacctggata agaagaatcc tgaagccatt aaaaagttgt tgaaagactc tggtacagat 15960
cctctggact ttaatccaga agaggagatt aactaccagg gtggcaaaca ccgtgttaca 16020
gacaccgaag cagattttgc aaccgaaatt gacgacttaa agtctactca agaaggacaa 16080
gctacattag gtgttattag ctcgacatgg gatgctgcca gtaaagatgc tctttatcag 16140
aatcgcggct tactccacac tattcacgaa cagcgtgaga atggtattta tgacaccatt 16200
gctaacgaag tgaatcgtct tcaggtgtta ggtcagattc cggtaggtac tccgttcatt 16260
caggcttata actacgttgg aaacctcctt gcacaacagg gagcatttaa ccaagtggct 16320
aagcctgaac cggtacaagc agttaaacca gcagttcagc cggtagtgcg tgtagcacaa 16380
cctaagcaga ctctggctaa caatgagcaa gctaaagcag cttcccttaa tcgggctgct 16440
acacgtaaag caacacctat tgtaaaccca ctcgctatga gtgatgaaga tttcgctaaa 16500
ctcccagtac cgggtagtct ttaaacagga atgagatgat atgttaaact ataacgctcc 16560
aattgacggt cagaaatcta gcattgatgg tgctggttcc gaccagatga atacctatta 16620
ctggctgaag aaggctatta ttcagtctcg taaagaccag tattttatgc ctctggctaa 16680
caccgttaac atgccgaaga actacggtaa gactgttaag gtgtacgagt atgttccttt 16740
gctcgacgat aagaacatta acgaccaggg tattgatgct aatggtgcta ccatcgttaa 16800
tggtaacctt tatggctctt ccaaagacgt tggtaacatc acctctaaac tgcctctgct 16860
gtcagagaac ggtggtcgtg ttaaccgtgt aggctttact cgtctggctc gtgaaggttc 16920
cattcataag ttcggcttct tctatgagtt tacccaagag tctctggact ttgattctga 16980
cgaccaactg aaagaacacc tgtctcgtga actgatgaac ggtgctgtac agttgactga 17040
agctgttctc cagaaagacc tgctggctgc tgctggtact gtactgtatg caggtgctgc 17100
tgtctctgat gctaccatca ctggtgaagg ttctaccccg tctgtagttt cttataagaa 17160
cctgatgcgt cttgaccaga ttcttactga gaaccgtact ccgacccaga ctactatcat 17220
cactggttct aaactgacag ataccaaagt acttggtggt actcgtgtaa tgtacgttgg 17280
ttctgaactg gttccagaac tgaaagcgat gaaagacctg tttggtaata aagcattcat 17340
cgaagttcag cactatgctg atgctggtac tctgatgaat ggtgaaatcg gttgtattga 17400
taagttccgt attatccagg ttcctgagat gcttcattgg gcaggtgctg gtgctcaggt 17460
tactactaac ccgggttatc gtacttctat ggtatctggt caggaacact acgacgtatt 17520
cccgatgctg gtagttggtg acgactcctt ctctaccatt ggcttccaga ctgatggtaa 17580
gtctgttaag ttcaccatca tgactaagat gcctggtaaa gaaactgctg accgtaacga 17640
cccgtatggt gaaactggct tcagttctat caagtggtac tatggtatcc tggttaaacg 17700
cccagaacgt ctggctctga ttaagactgt tgctcctctg taatagtccg aaataaaaca 17760
agggggactt atgtccccct cttaataaaa ctaaatgaac cacaggaaat atgaacatga 17820
gcattaacga taaaccgacc actgacgtag aagtgaacgt acagggcaac gaagataccg 17880
ttaacgatat tgcactgcct agcgaacttg aagttcttaa gcaacgtgca acgttgatga 17940
atatcaagtt ctctaacaac atttccgttg agaagcttcg tgagaagatt gaagctgcac 18000
aagtaaaaga tgaaccagta gttgaagaag ctgctgttaa cccacttggc gaaaaacaag 18060
aagctggcgt taagaagatg actctgggtc agaagattcg tgcagaacag actcgactga 18120
ttcgtgtacg tatccagaac cttgacccta agaagaaaga cctgccgggt gaaattatca 18180
ccgtagctaa cgaatatatg ggtactgtac gtaagtttgt tccattcggt gaagtaaccg 18240
acaatggtta tcatattccg tactgtatct acgagttcct taaagaacgc aagttcatca 18300
acattacgac tcgtaaaggt aagaatggct taccggacat ccgtgcaaca gaagcacgtg 18360
agttctccat tgaagtactg cctccgttga ctgaagcaga actggctcaa ctggctcaag 18420
cacagattgc tgctggtagc cttaacgatt aacgtataag ctacagttca acacggctca 18480
ctggggtaac tccgtgggcc gtttcttttt gaaccaagag gaatgattat gtcttgttct 18540
gctgaggtag aagctaaccg actcctagaa gagttaacta agggtgatga ctttaccctt 18600
cctgatattg atatgtctgg tcctgaatgg gatatccctg gtggagatga ttctccaatc 18660
tttggggcta ttactaaaat aaccaatgaa tctcttacta cccgtgaagt aggaggcagt 18720
ggtacgtttg atgcattaat ggaatctgct cacaatcacc ttaaagctga gtttaaagct 18780
aaccgtatta ctggtggtga atataccaaa gcatacattg ctatgatgga atcatgcatg 18840
agtaatgctg ttcagttctt acttggacgt gaccaggcat actgggctgc tgctatggca 18900
cagattcagg cagttactgc tcgtgttcaa ctggctactt ctaaggctca gtttgtactg 18960
gctaagattc aggcattgtc tgctaagagt gaatacgctc ttactaagat gaagattgct 19020
actgagtctg agacgtattg tgctgcattg tttaatgcta atcagatgtt acctcaacag 19080
cttaaattgc ttactgagca gacagaagca caacgtgcac agacactgga tactcgtagt 19140
gatggtgcta ctgttactgg ttctgtaggt aaacagaaag agttgtactc tcaacagatt 19200
acttcttacc aacgtgatgc agaagttaaa gcatctaagt tgtttactga tgcatggatt 19260
acacagaaga ctattgatga aggcttgaat cctcctaatg gtttcactaa cgcaagtatt 19320
gataccattt tgaccaagct gaagactaac aacgggctga actaatatgg gcttattcag 19380
tgggaagact aaagtatacg tagcatcctc tgtctataac atggcagggg atatacacaa 19440
acgtcctaac tatatgaaga ctgtagtcat cggtaacatt gtttccaata caggattcac 19500
tatggcagac actatctctg atagttatct gaatggacct ggtattcgta tgcgattgtt 19560
ctccagctgg agtaagaacc actatgattc agaagttggt atggctggtt cttcgttggg 19620
tgtattggct aagattgacc ctactgttat agagggtcaa attacaccac ctgctggaga 19680
gtctgtctat gttcaggcag cagaaattgg ctttgctgac tttgagcaat ggtgtgacca 19740
atatctttat gaaaatgctc ctagtcgaat catggaacga ttcgacatag acattgatga 19800
agataccaat gaaattacta tgacctcctt ggatggaggt tctactatta aatttacacc 19860
tactaacttt gaaccaggtg ctttgtatct ctatgctgat tactcattct ataagtcccc 19920
gtctatcaat ccaccggtag agggaccgac tactgtctat gatgatgagg atgacctacc 19980
ttctactttg ttatggacta ctgtttcaag cgatacaaca gctaatacaa ctaatctcac 20040
taagactata catacacatt ctgtttactc tgatggtagg ccagatgaag acagtactgt 20100
tactgaacca agtgtggata actggaactc ttacattaag gtttatcgta agggattcac 20160
tgaaaaacct gatgacgtaa ctgtagttgt atctgacagg gtaatgactc ataaaaagtt 20220
tggtgaggtt caatccagta ctactgtaac taccaatgta gttgatttag gtggtggtgt 20280
tactcgtacc gatacaatca ctacaaccac agagacactg gttcgtaagt attcctccca 20340
gactactcgt acagatacag agatgactgc tgttggtaca cctaagctgc ttatctataa 20400
gcagaactct ggtaacgttg cactagactc actgttcgat accgaagcta ctgataaccg 20460
attctaccca ttcattccaa tcaggaatga taagcaatgg gtagagaatg acccaatcta 20520
tccatactgt aagaaagctc tttataaagc taccggtgga aaaattgata aggtagtaac 20580
agagcttaag aagaatgatg atataggtga tattcagtac atttatggca catttggcgt 20640
atctcttaat actcctgaag atactgctaa ggaatacatc taccgtttct ttcagatggc 20700
tactgaagca ttcccacctg acccaaccta tccaaccttg gaggcagtga ttgcaggtta 20760
tcaggcagct aatgatgcag ttgatgcata tgtagaatgg tggaatagga ctgaagggta 20820
cactattgga actcctccac cactgccaga atacccagtc atacctaaga gagaatttag 20880
ggtacatagt aataagcatt acaagtatga catgaccatt ggttggaatt acgtatctga 20940
aactacccac tcagggcaag catggtctgg tgctaagcaa ggacaacttc gagccaggta 21000
tgcaggggat ataactctta ctcgcaagaa catacgaact gctagtaact ccggttcagc 21060
taagaagtta gaggttacca ctacattcta ttctatgcag gagtatgaac tgctttggca 21120
ggatggtaat aacacttacc gtaaactcag agtactgggt ctaaatcaca ataacagggt 21180
atataagaat aagtcagtgc atattgatgt tgccgaagca atgggtgatg cagaggagag 21240
tgggttcatt attccactac atactaacat ctaccgttca atgtcactgg ttcgtagtac 21300
tcagttatct actgcctgtt cttacttggt gatgaactca tataagaagg ttaagcagaa 21360
gtggtatcaa acatctgcct ttaagattgt ggttgttgtg gttgctgtcg ttgtatccgt 21420
attcactatg ggtactggtg gtgcaggtat acttggggca tatggagcag ttggggcgtc 21480
actcggtttt gttggattag ctgctgtaat agtaggtgct gtagcaaacg ccattgctgc 21540
aatgattctg gtatctatta tcactaaagt atccacttca ctctttggtg ataagctagg 21600
ttttatcatt gctgctgttg cttcaatggt agctatgaat gttggtactg ctctatctac 21660
tggttcatcc ttatctacct tagccagtga aatgatgaat gctcagaaca tcatgcagct 21720
taccagttca gtaggtaatg gtattagcca gtatattaat gcatctacgg ctgctactat 21780
tcagaaagca gagaaagtaa tgcagcagta caatactgat atgatggcaa tcaataagca 21840
gtatgaagag atgtttggta ctgctgggca gggtgtaatt gacccaatgc aattcgtgtc 21900
aatggagtca ctggattcat tcttatcacg tactcttatg actggttcag atatagctga 21960
cctgtcttta gacatgattg gcaactactc tgacatgacc cttaatactg accttgttta 22020
acaggagaat ccctatggat ttgtcattct taacaggcaa ccaaactcca cagccagctg 22080
ctatgtcttg gatgtctaat aacaatcaga actttaatac cccagctatg aactataacc 22140
ctggtgcagc tattggtatg ggtacgaatt tgggtgcatc tacccctact tctttcatgt 22200
ctggttcagc tagtcagttg ggcatgaaca tacctacctt ccagttagga cttggtgcac 22260
ttggttcctt agctagtatc tatagtggtt tccaggctaa caagttagcc aaagaccagc 22320
tgaactttac taagagtgtt actaacacta acctgaataa ccagattaag tcttataaca 22380
ctgcactgga agaccgtgct cgtagccgtg ctgttgcaga gaaccgtgat cagtctactg 22440
ctgatgctta cattgcagct aataaattaa cccgttaata ggaaaccatc atggctcaaa 22500
ttacatggcg taatgttgat gcacctaact tctctggagt tggtgacagc attcgcacat 22560
ttggcaacat gattggtaac gctacctccg gcttaagtga tgcactgggt aatttccaga 22620
acgctgccaa gcaggaggct ggcaatgccg tcatgatgaa tgctatgcag taccaagacc 22680
cgaatgagta ccgtaatgct ttggcatctg gtgcactgtt tcagggggtt gatccttcac 22740
tggtaaatca gcgtaccctc caaaacctgg atgaacgagc aggtacatta ctgacacaac 22800
aaggacagca gggaaccaat gactacattg cttatcgctt tggtcgttta caggatacta 22860
acactgcatt agataatgct tccccggcta ttcgtttact gtctgaggct tatcagtccg 22920
gtgaccagaa ccgtattaat gctgcattaa gtcagacccg tgatgcactg tctgcactcc 22980
ctgctgacca acaactggag atgatgggtc gtctacaagg tcagggtggt caggctatta 23040
atcaggcaca gaatcgtttt gatttaggtg ttgctcaacg taatgatgct gacagtcagg 23100
cagctatggg tgtaatgtca cagattaccc gtggtgctga gaaccctaat gatgcccgtc 23160
tgttggcaga agcatacagt aagaacttgt ccccagttgc acaggcacgt ttacagagtt 23220
tactggctag tacttacccg ggtgtatacg gcaatggcgt aggtgctgcg tctgctcctg 23280
gtactgctgg tactcgtcag ggttcccctt acgatgttac ttaccagttt gccggtacat 23340
ctacccctat tactcaaatg agtattggtg atgtaattaa acaccaggat ggtatgaagt 23400
cgaatcaagg tgcttcacct gttggtgcat tccagattaa ccaagctact ctgaaagact 23460
tcgctcccaa agttcttggt tcagattggc aaagccagcc tctttctcct gagaaccagg 23520
ataagattgg taaggctatc ttcgagtctc gtaagaacgg taaccttaaa gatacttggg 23580
ctgctctgcc taactctacc ccaggtgctt ataaggatat gacttgggaa cagattaaac 23640
ctgttattgc tcaagctgaa gtaggggctg acccacttgc tatggttcag aataatcaag 23700
gtaatcaggc agtatctact ctggctcagg gtatgattgg tactcgttct atgcaggata 23760
acactggtag tcttaacaca gacttccttg gtactgtcac tgataatacc ccagtaggtg 23820
agattgctaa tcgtttgctg gagggtgatt ttaaaggtgc tgaccgtaac tgggtactga 23880
atcgactgaa cgatatttct caacgtggga atatgtcacc tgctatggct gctgctgtta 23940
tgcaacgtgc acagactggt gtaccagagg gttatattag tcgtgctatt gatgcagtta 24000
acccattcat taccaatgaa gcaggtaatg gtatgcgtct taatgaccgt caggtagaac 24060
agttaatcga gggtgtgaaa cggggtgaac cactggaagg tagtgttcgt aaccttggtc 24120
gtgctcaagc tatgcagaat attcaggcgg cacagactgc atatgatgct gctgtagctg 24180
cactggcaaa tacccagaat aagattaaca ccggacaaag cggtcttact gctcttttac 24240
ctgcacgtca ggaggctgtt actaaagctg ctgcaatgct ccaagctgca caggcacaag 24300
tacaggctga cccagctaac ctggctccaa gaggtttcca gtcacaggct agtgtggata 24360
gagcacgagc caaggaagat gaagaacgtg ctgcacggta cttacgtcag gcacaaggac 24420
tgcctgagta tatgcaaccc agacaaaatc ggtaatacta ataaaagccc tacggggctt 24480
ttttctttaa ctggcttact gtagagtaag ttacctatat acgatataag tttgaacagg 24540
aaacatgcta tgtcaacatt tgaccgtctg gcaggtttcg cagacagcat cactaatgct 24600
aagcaagtcg atgtctctac tgcaacctct cagaagcaag ctgcacaagg gcaaagccct 24660
ttccaattaa ccccagagaa tgcttacaat ctccagactg gacgagtagg taatcttgga 24720
atcggtgcat tcgaaccagg ttcacttcag gcagatttca ctcaagcctc cccattagaa 24780
atcattaata agtacggtaa tgagcaaggt ttacaaattc ttaatagccg agctaatgct 24840
gctgatgttg tacgtcagga cttatctaaa caacgtacta acgcagaagc atttggcgat 24900
actctttctg gtgttggtct tggtgttgct aatacccttg ggggcattgc tgctcttggt 24960
acaggcttgg tcaatgataa tgccggggca tccattgctt ccggtcttga ctggttgaat 25020
aaaggtgtac acaacctaca gtctgatgca ctgaatgctc gtcgtaatgt agtacagaac 25080
cagaacgtaa tatctgctca agagaatgag aaactctata agcaagatat tgctaaagga 25140
gaaagtgaac tcgttgcttc tctgtctcgt attggacgag atgcctatga ttctgttgct 25200
aacaccctga ctaatgacat ggctgctaca gatggcctgg ctgaaggtgt tggttcactc 25260
tttactggcg gtccacttat ccgtggtgta tctgcacttg gtaaagtaat ggtaggtggt 25320
gataaagctg ttaagggtat taccttagct gctgaattag gttatcgtcc tgcacgtatt 25380
gcacagactg ctggtcgtgt tgctgcacct gctatagcaa ttggtggtat ggaagctggt 25440
ggtgcatacc aacagactgc taatgaaatt atggaaatgc cattcagtga actggctgct 25500
aaatctcctg tataccaaca gcatattaaa gacggtctgt ctcctgaaga agcacgtcgc 25560
cagactgcat ctgaaacagg tatcactgct gctggcttaa ctgctccagt tgctgctctc 25620
actgctccac tggtatccaa gtttgaactt aacccactta aggtaggttc actggctggt 25680
gctggttcta acatgctgcg tgagactgta gaagaaggta tccagtctgg tacaagtcag 25740
tttgcacaaa acaaggcaat tcatggcaat gtagatgctg agcgtgattt gcttaaaggt 25800
gtgggtgaac aaactggtct gggtgcattg tatggcttcg gttctgctgg agttgctcaa 25860
gctcctggtg caactgttaa ggctgctggt gctgccgttg gtcctgctgt acgtactacc 25920
ctggctggtg cttctttggc tggtaaggca ctagcaaaag tagcttcacc tattactaac 25980
atcctggttc aacgtgggga agaagttgct aagcgtaatg aacaagcatc ccccgtatct 26040
gatgctacgg ttaacgctgc tgcacaagaa gctactgctc aggcagaaca agctcaagca 26100
actgttcagg aagcagtgga tgcaatggat atttctccag aagagaaagc tgctgctacc 26160
cagtatgctg ctgacttaac tcaggcaatg aagtttgacc cggtagaatt ggaacaaact 26220
aacccagcaa tccgtgaagc tgtttctggt tccactaacc gtgtagaagc tattcagaag 26280
atggctgact tggttaatac tgccgaagac cctaacgttc aaatggaagc tgcaatccag 26340
atgtatgaca atatcatgtc tatggatagc ttcattaacc gtgacccggg tgctttggct 26400
aatctggaac aaggttcccc tgctgctgca atcgtagacc aattctccgg cctgatggct 26460
aacgtacaga atacccctaa agtactccgt gcatttcgtg ccatccattc catgattcag 26520
gaacaggctg aagcgggtaa gctgaaagct actgaagaag ctaatcaagc ccaagcaaac 26580
aacattgcta tggctgctga tgtatctcca gaagtgctgg atgcagattc agtgaacatg 26640
gttctgaaac atgcaagtga aggtaagatt acactgggga atcgtcaacg tgctgccctg 26700
caatctgctg ctgccttatt acagggtgca cgagagttcg acgctaaagc agaagaacta 26760
ggcttacgtc ctcaagatat tgttagtaaa cagattaaaa ccgatgagtc tcgttctaat 26820
gaaggacagt actctgcgtt gcaacatgcg aaacgcattc gttctgcgta taactctggt 26880
aacttcgatt tggcctccgc ttatctgagc gattttatga agttcgccca gcacatgcag 26940
aataaggtcg gagcgttgaa tgagcatttg atttctggga atgcggataa gaataagtct 27000
gtccattatc aggctcttac tccctcgcgt gaatgggtac gtagtcgtac tggcctcggt 27060
gtcaatccgt atgataccaa gtctgttaag tttgcccagc aagtaggact ggaagccaag 27120
accgtagctg atattgctaa cgcacttgct acggcttacc cggagcttaa tgtttctcat 27180
gtgaaagtta cctcgttgga ttcacgtctg aacaagccag ctgcccaggt agttaaagag 27240
ttccgtcaag gtggtcttga tgccactcag tctcaacaga ctaatgaacc agtgaatcag 27300
gttgatgaaa ctcctgcact agtgcaagag gacaccacta ctgtacagcc taaagataaa 27360
ccagtggtag aaaataagac tgctcctact aaagagacta gtccaaaagc agaagaagtc 27420
tctactactg aatctgaaag agatacttat gtgaaagaaa atactatttc acaggaaact 27480
cttaataagt atggtgaccg taaagagagt ccatggctct ttgctgatgg tactgttgtt 27540
ggttctaatg gtgaccatat cagtctgtct gatgtatttg gtcatgatgg ttaccgtgat 27600
atgtctctgg atactggtgc tgtacgctcc actttctaca aaggaagtga gcaaccattt 27660
gtatcattac aactgtttga taatcagaag ctgactcctg aacaattcaa tactgttcaa 27720
tcattcgtag aagcaaacaa tgcactgatt cagattggtg tagatacgtc tgggcgtaat 27780
accaataatg tagagaagga agaagtatca ctggctcagt tgaaagcaga ctataccgtt 27840
ggtggtaaac ctttatctca aatggaaaca gtgttccctg tctacaacaa tgacaagaca 27900
ccgaaccagt tccttaaagc atttagtctt ccagaagaac ctaagtctcg taccattggt 27960
tccgaatctc ctttgactga tgttaagaaa gcactgtctt ctgctgctcg ctttgaagca 28020
tttactcaaa aagaaaataa cgctctcaca agtgatgtga ttaagcgtta ccagggctta 28080
tttgagtttg gtgaaactct gaagactact ctgtctgacc gtctggctaa gttccttgct 28140
aacaagaacg ttggtaaacg ttttgcggaa ggtactgagg ctaaccgttg ggtaggtggt 28200
aagttactta acatcgtaga gaaagatggt gacaccttta agttcaatga ccagctatta 28260
gaaactgctg tacttgctgg tctgcaatgg cgattgactg ctacacagaa tgctgctgtt 28320
aaagacatga aagacgtagc tgctattact ggtgtggaag catctctgct accagaaggt 28380
attctggctg acttcgagaa tgctcagacc ctggttgaag ctactaactc actagctcaa 28440
aagattgaat cctattgggg tttgaatcgt aatcctaatg cacctttggg gtataccaag 28500
ggtatcccaa tggcaatggc tacagagatt ctgtcttccc ttattgagat gggtgaagtt 28560
aaagagtcca tgttagatgt gtctgagatt gacccagaat ctaataagac tgttggtctg 28620
tataccattg agaagctgga tgatagcgat gccattaata aattccctac tgctattgag 28680
gaaactgtcc ttttagaacc agtagagaaa ttgtatctgg gtgatgacat tccttccgtg 28740
gctcaaactc agttacgtaa cccggctgtt aagaataccc cggaacagaa agctgctctc 28800
aaagcagaac aagctactga gtttcgtgta catatgccga tggttaattt ctatgaagca 28860
ttgggtcgtg acaacattct ggagttaatg ggtgctggtg ctcttaatcc agaattgctg 28920
aatgtgaata ctgctaagtc tctggaaggt aaaaacctgt ccgtgtccat ggcgtatgat 28980
tccttgtttg gtgtaatcaa tcaaattaag gaacaggaga atggactgga tacacctatt 29040
cactacggct acaacatgac ccgtgtaggt cgtatgcaga tgcttggtaa gaacaaccca 29100
cagtccagca aactggttcg tgaagcaatc cttccaacct tctctaccat cgacttaagt 29160
aatgaaaaca gtcaaacgtt ctctgacttc cagcttggac tggctcaggc attaggcatt 29220
aaagttcata aaatgtcccg tgaagttatg tcagatgagc tgactaaagc tctggaaggt 29280
aaattgaaac ctgctgttga tatgatggtt gagtttgata agtctggaca cttacctgct 29340
gatgcagtgg atattctgaa gacttctctg gggaatgaca aatcattcgt agctctcatg 29400
gccctgatgg aatatgcccg ttaccttaac agcgatgacc gtaccaactt taatacccca 29460
ctgtacgttg aagctgatgg tgtaaccaac ggaccaatca acgcaatggt attaatgact 29520
ggtggtaagt tcaccccaga ctggattaag aatactgcca aaggtggttt gttcttcggt 29580
aaagctggta agactatgaa cgagcatcat tcgcatgagg atagtgttga cctgtatgaa 29640
gcatccacta atagtctgca acaggcactg aatgaattgc gtaatactta tcgtaacaac 29700
gttcctgtta cgaaccagat gaagcatctc cagaagttaa tggacctgtt cattaaagac 29760
ttcaatttga atgaagatgg tactctggac ctgaaacgtg gtatcgctaa aaacccactg 29820
actattacca tctatggttc tggtgctcgt ggtattgctg gcaaaatggt aagtgcaatt 29880
actgatgtca tctatgaacg cttcagtgac gttctacaag cccgtgctgc tgaccctagc 29940
atttccccgg ctatggctat gtttggtaag gaggctgcat ctgaggccga tgctcaggct 30000
atgctggata ctttccttac ttctatggaa gcactgactg gtaatgttcc ggttatgcgt 30060
aaaggtgaat tgtctattca acaagtagag ggtgctctga ctggtaaatt agaccctcag 30120
aagtttaccg tatctggtaa tgccctgaag aacctacaag agaacatgct tcacctgttc 30180
gttgaaccaa tgcgtactgg tattcgaaat actgttggtg aaggtctgat gtactctact 30240
gagaatttgc agaaggctac tcagattcaa tcactggtat tgcaggatat gttccaacag 30300
gcagtacagg ctaaactggc agaaaaagaa aaagacccaa catggaagaa gggtgatttc 30360
ttaacacaga aagaattgaa tgaaattcag gattcattat ctccactggc tccaatgatt 30420
gagactggtt ctcagacttt ctacattgct ggtagtgaga atactgatgt tgctaaccaa 30480
gtactggcta ctaacttaga tgaccgtatg cgtgtgccaa tgagcatcta tgctccttca 30540
caggcaggtg tagcaggtat tccatttatg accatcggta ctggtgatgg catgatgatg 30600
cagacccttt ctactatgaa aggtgctccg aagaataccc tgaaaatctt tgatggtatg 30660
aacattggca ttaatgacat cactgatgca agtcgtaaag ctaatgaggc tgtatatact 30720
tcctggcagg gtaatcctat caagaatgtt tatgactcct acgctaagtt catgaagaat 30780
gtagacttca gcaaactatc tgatgaaacc aagcaagcta tcgctaagtc tgctctgaaa 30840
tatgaccagc gtgaaggtgc tactgatgac ctgttacgtg ttggtgctga acagattgaa 30900
cgtaacctac gtaacattgc tctgggtgta gatattcgtc acaaggtaat ggaccaggta 30960
caggttactg ttgaccagat ggctgctgta ggtgctcctt atgtgaacaa cggtaaaatc 31020
tctttggaag gtcttactgt agaccagcaa gtagctaagc tgaacgaact gtttgatgct 31080
gaattaaaca agcgtcgtga tgcagtacgt gctgctaaag cagaaccagt gaaagaagtg 31140
cctgctatgg aacaggtagg tcgtgtactg aagtctggtg tacgtctgct gtctaatact 31200
gccattgcta agctggctaa agagatgtct cccgaacagc aagcagtact gaaagaaata 31260
cagaagtctc tcactgctaa agactacaag gttgtttatg gtacaccttc tcaactggat 31320
gcttatgcta ttgagaagaa tattactcgc ccagcaccag aagatattga agctgctgaa 31380
gctggtaatg catatggctg gactaacttt gaagataaga ccatctactt agttacccct 31440
tccttagaga ctctggttca tgaattggta cacgcttcta cttttgaatc tgtattggct 31500
cactatgagg gtactcctaa cgaagctgtt cagaatatcg aagacctaat gaaccagttc 31560
cgtgctctgg atgttaagaa tgaatctccg gcagtacgtg aagcatatgc tgatgcactc 31620
aataccatta atggtcacct gtctaatggc tttattgaac cagcaatggc taaagctgct 31680
gctctgaatg agtacatggc ttggggttta accaaccgag aactgattag taagcagaag 31740
aaaactactg cacttaaaac tatggttcag actgtatatg aagcaatcaa acgtctggta 31800
tttggtcgta agaaagctcc agctaatgca gatgatatgt tctcaggctt actgtttaac 31860
tctgctgtag tgatgcgtgg gcaggcccct actactgctg tagctaaaga cactactctg 31920
ttccataaca aagcatatgg tgacaatact cgtctggaag agctagggaa aaccttcgat 31980
aagctaatta ctaactacat tggttcagaa ccagtacagc aagtaattcg caaaggtaag 32040
ttctctgatg cagtagtaaa tgcaactaaa ataactcgtg atgttcaggc tcatggcttc 32100
accatgaata tgcaggaaca gcgtctattt actaacattg ttgctgcact ggctaccgag 32160
gctgctatta accctgctgc tatggctcgt gcacaggaat actacacgca tgttactaag 32220
aatctgaccg tcgaagcatt tatggctgac ccagatagta ctaaccctgc tgatcgttat 32280
tatgcacagc agaagttcga taccatcatg ggtgctaaca acattgaatt tgatgcacaa 32340
ggtcgttctt ctttactacc tacctttgtt ggtttggcaa tggtaagcga agaggtacgt 32400
aaggtactgg ctgatattcc tgttatgaaa gcagataaga aatctggcaa tactgttgac 32460
gcaatgctga ctaacatcgg tactgctgca atggctagtc tgaatgctcg tgtagcaggt 32520
gatactaaag ccactaacgt acaggaaggt atggatgcac tggctcagac aatcatgcag 32580
accagtctta atgcagagtc cttctatgat tccgttgcta ctcctactgg taatgctatt 32640
gaccgtgcta atcagtatct ggtggattct attgatgtac tgtctacccg tgctctggaa 32700
ggtgctcgtg aagttgcagc taataccaag aatccactag ttaaaggtgc tgctcatgct 32760
gcacaactaa tagctgctat tgctacagag aagaatggtt caatcgtagc cgaaggtgtt 32820
atggctgcaa tgaaccaggg taaggtatgg caacctttcc atgacctggt taatgacata 32880
atcggtcgta ctaagggcaa tgcttccgta tatgatttga ttaaggcagt tcgggcacag 32940
gtgcaagctg accgtcaaca gttccgtgag catttgccta ccactatcgc atctaagttc 33000
agccgtaagc tgactaatga tgagtggaaa gcaatgcaca ctggtatggg taaaactgac 33060
ttagctgttc tgcgtgacac tatgtctctg gatgaaatcc gtgacctgct gactgaccaa 33120
aagattgttg accgtgaagt taataaactg gaagcagatt tgcagaaaca agcaggacgt 33180
aactggcctc tgattagccg taaatctaag caactggctc agtacatgat taatggtacg 33240
gttggtaata acctgctgcg taatgctact gcaatttctc gtttgctggg tgaacgtaag 33300
actacacgtt ctgttgtaga tgtagctcaa ctggataaac tgattaccct gtatgcactg 33360
gaagcaatga ataagactga ccgtgagatg ttatcttcac tggctcagac agaaattgaa 33420
ggcatggact ttgctacttc ttacctggtt ggtcagcgta aagatgaaat ggctaaagct 33480
aagtctgata gtcgtgcaac cctgaaccag tacaaagggt atattcctag tgagaataaa 33540
cagggtgtta acctgattgt tgctgaagat aatcagtttg ctgatttgat tgagaagtct 33600
tatgtgcgac tgggtactta tcagggtagc tctgctaacc gtggtccagc acgtagttac 33660
tactttgcac cagtacaggc acaggcccca ttctcacagg gtatcttaca gaacgttcgc 33720
aatactgctg gtggtgtgga ccttggtaca ggctttacta tgggaaccat ggtagctggt 33780
cgtattactg acaagccttc tgtagaccgt atcactaaag cactggctcg tggtgaacgt 33840
ggtaatgaac cactgctgcc tgtatatgat gctaatggta atgtggttgc ttacgagcaa 33900
tccgttaacc ctaatatgct gaagcacatc gagggtgaga accatcttgc tcgtgcaatc 33960
ggtgtatggc gtggtcgtca ggtagaagag gctaaggctc agcgtttcaa cgatatgcta 34020
attgagaacc tgcatagcat gtatgagaac gacattaaga tgtctgcttc taataagtcc 34080
cagtacgtta accttcttgg ttctaaactg gatccagtga ctgccgatgc tcttaaactg 34140
atgaacagtg aaacacgtca gaaagcagaa agtctgtttg gccctggtga gttgtgggta 34200
cgtcgtgaca tgctgaatga tgctcttggt tatcgtgcag cttctgttgg tgatgcctgg 34260
tctggtaatt ctcgttggtc cccagaaaca ctggatacct tcaagaaagc tatgttgggt 34320
gtctttggta accgtgccta taaatatgta atgggtggtg agaacctggt tcagaactta 34380
gttaaagaag ctaagactct gattgtggtt aagtcagtag tcgtacctgc tgttaacttc 34440
ctggctaacc tttatcagat ggttgctcgt ggtgtacctg ttaagaatat tgctaagagc 34500
atcccacaga agactgctga gattaaccag tacctgaaat ctcgtctgcg tcaggtagat 34560
gcagaagcag aattacgtgc agctaccaac ccgaaccaga ttcgtaaact taaggctgag 34620
attcagtcca tcacggatag ccataagcgg ttgagtatct ggcctctgtt ggaagcaggt 34680
gagttctctt caattgcaga tgctggtatc ggtcgtgatg atattctgat tactgaaggt 34740
aaactccacg aatacatgga taagcttgct gataagctgc ctaagtctgt acgcaacgca 34800
ggtcgttatg cacttattac taaggatact gctctcttcc agggtatcca gaaaacggtt 34860
gagtactctg actttatcgc taaagcaatc atctatgatg aactgaccca gcgtaaaggt 34920
aagactcgtg aagaagcact tggacgagta acggaagagt tcattaacta tgaccgtttg 34980
cctggtcgat tccgtgggta tatggaaagt gttggtttga tgtggttcta caactttaag 35040
attcgttctg caaaagttgc agtgtccatg attcgtaata acccagtaca tgctgctatg 35100
gctatgttgg ctccaacccc taccatgttt ggtaacgttg gtcttcctat tcaggacaac 35160
atctttagta ttgctgcaca aggcaatctt ggttactcct tcggtttcgg tcaaggctta 35220
cgtgctcata acctgaaccc atggctcaac cttactaact aaatgaaaaa gcccctcatt 35280
agaggggctt tgctttagaa tcgagaaatt cgtaattcaa gtatacgggc atacgcagac 35340
atagcatcta attgtgtgcg tagtaatgcc tggtcaaact gatccagttt aatgaatgtt 35400
tcatttgtat taataaatac ggatagatca tgtatttttt gattcagtgc acccagttca 35460
tctaatacac gaagttggtg tgctgcatat aatttagtca tgcgttctcc acatcttagg 35520
cttacgccct tcacttggaa atttaccagt ctctttaatc ttccgtaatg tttcctccga 35580
tgggaaccac aggaatgtga agtaaagttt cttagctacg taacgagtta ctcctagttt 35640
ctctgcgatt tcatcaaatg aatatgtacg aacggtattg ttgagaatgg cctcacgaac 35700
aacagaggta atagatactt gtgtcatgct attttcctta tagaattacc caatcttctg 35760
ctaatacatc agattgggat ggaacccaag gcatgtattc attatcaact gttttgatac 35820
caatccatgg gcgtggctta aggtcttcat cccatggacg ctctagttca ctactattcc 35880
actcttttgg gtcaacgtag gaaatataca tccctttgcc attccaacct ttacgggcta 35940
gtttgaagcc tgatttgaga agctctactg catgaccaaa ggtacaacct ttttctacgt 36000
catggtatgc agcttcaaat gcagctttag gtgaccagct aatatagcca cggaactgag 36060
aataaccacg aaattgaggt tggttggact tagagtctgg gtactcgact aagtaacctt 36120
cttctagtgg atcttcatct gctggtacat cccagcctcg aagtttgcag tatgtttctt 36180
tgttcattcg gaaaccattt acacacttgg ttccaatgta gttggtgaaa tggactttaa 36240
tttcttctga cataactacc tcaaatgaga aagccctcac taggagggct tgttttgttt 36300
tggcttctag agccttacta ctttgtcggg tagttcagta cctttcggta aaaactacct 36360
tacacctttg aatcagattg tacaaccacc accatcacag gtattgtttt ctgcaaaaga 36420
agccagagcc ttagcagtct cctctttctc atgcagtgcc tgacgtagca ggtaaccctc 36480
taactcccaa atcttattga atgcattttc atatgcatac tgacggccct tctgctcatc 36540
atagatagta ggatcaacac aggcagatac gccttcaact ttgaagccgt tttccaggat 36600
gataatacaa tgggttgtca gacccaggtt agccaaatca tctgccggga gtgcagagga 36660
gtcaacgaat gcagatttca ctgcatctgc accattgaaa tagtagacgc tcttaatctt 36720
agcttcgatt gcttctttac ttactgttgc cattattagc ctcatagagt ggtttgagtt 36780
tgtacttaaa catgactgcg ttgttatagg ctacgctacc tgcaaatacc aagtacttat 36840
gtcctcggtt atccgttacc tcataggcat acggagtaga aattaggtca atcattttga 36900
atgtccaatt attaatactc aaataaaaaa gtccctccga agagggactt agtagcttta 36960
acgcagctaa cgaggctagg ccagttaact attaccaccg gtgcttgtac cagcaaacag 37020
gattgatagt ccgataatag ttaactgtaa ttgagcatta tagtctgctc atactcacac 37080
cgttaggtat gttttcaata attagagtgc cggactgtct caccggcatt gtctttagag 37140
tgcaatgcaa tcaggactat gttctctatt aatgagtacc cactactgga ttcgaaccag 37200
tgaccacaga caccttgttc tctcgaagct ttcctgcgct ctaccactga gctaagtggg 37260
cataaatgag tgagtcatgc caggctttaa ctggctcctt cttcgcaatc cttacatacc 37320
cctacataat tattgcgtta agcttttttg tttaagttta aatgaaggcg ttcttgctga 37380
ctcataatag ggtgggcgtt tgacgaatga ctagttcgtt actacccatt tgccataagt 37440
acctggcgac tttggtttga ggtgggagtc cgagtcctgt acagccttac cgttcacaca 37500
ttggcagcgt atctagaata gcacttccgg caactggatt taactgttac acctcaaata 37560
ggcagtgggg gatggaattg aaccaccagt attaagtctt attcgtgcac caagcacgct 37620
agtagattac tctaccggag ggaatcgaac cctatctact tcaccagacc caccataatt 37680
cttttaattc agagtagcac tgctccatgt ttggcctttg aactccggga tgggttagtg 37740
caatgctact ttgaattggt tatcagtgaa ggattcgaac ctccggttgg agtctatagc 37800
ttaccgcagc agccctccaa tctagcggtg tcacacttag tccaaacttg aacatgtcaa 37860
gccatctcgt gcaactgata attggttccg gcgtctggac tcgaaccaga acactcatgc 37920
cgtgcacgac attagtacct acaccggaat tagttgccag ggattccacc tggctccatc 37980
tgtttttata gtcactcaga tatcgtctgg acttgttgtg gtggccggtg ctgatctccg 38040
gcataaggcg cttattcggc ggcgtcgagc ttgcttattc gcttgagcca tcctcgtgag 38100
tcagccacgt cccactgcgc atcagcctgc gcattcacca caacggaaag agcacttaac 38160
agaagatagg tctgattgcc tcggatgatt tctcagtagc ctaaatgctc ttacctgttg 38220
tgtgcccatt attaatcaca gcgggccact gcgccgaatt tgtttacaag gaatcggaag 38280
accttgctga cttataggct attacgccgc catcagaaca acatcatcgt ttgcatttat 38340
ctttgtggtc agtttctaaa aagtccgcaa agtcgcttac gaaaactatc aaagagtaca 38400
ctaagcacag gtagctaccc tgccgtcttc tccacctaca ccggctacag tgtaaacctc 38460
cgcacgttag cttaatgtac tcattgatag gatgtcagta ggtcgggggc tggattcgaa 38520
ccagtgaaag aagtagcgac catctgcaca tgcctacgtg ctaccccgac cattataata 38580
ggagggaggg gacgagtcct ccctttatac cctatattgc atgtaagacc taacaattcg 38640
atgctgtagg tagctatgct atccgacatt taacccagcc catatagggg cttatgtgat 38700
taaaatgctc aacattgagc ggttagtttg ataagggatc tgaagcctta tcccaaagat 38760
actaaccttt gggatatagc ctatcattca gaaatggccc ctatagggag aggccattga 38820
ttaacccgaa tgttgcttta cattttctgt gataacccat tcttaaaata gtctgtctga 38880
cggatggggg gccaccccta gcgtcagtgc catacggcaa agattaagtt aagaaaggtt 38940
atgataggag ttcctatctc aaaggattct aacagaaccc ttcacgatat taactactct 39000
tttttaatgc ttctacttca ccctctttat accaagcata tggtactaag attttgcagc 39060
cttctgattc aatgtgaaat gctttctttc cttcaacggg ttctccaccc atgaaagaac 39120
taacctttcc attgagccag tgaatagtaa atttgttcat attagcctca gttgaaccaa 39180
tgcacaggag agagtattcc tgcacactgg ttcggttgtt gttactctgg cggcttgtca 39240
ccaccgtgtt taccactgca tttccacagg ataatgacag tgatgataaa agctatgtat 39300
ggagccgaca cgtctataat cctgaccagt agtagcatca ggattataaa cactaccaca 39360
cctagcagca gcttctgagc cacctaccag attaactacg tttaaggcca gcgaacagtg 39420
aaggcttagt agcaggtttt tctgctggtt cttcggcctt aacttcttcc tgcacaactt 39480
cttcttgtgc ttctggttca gttactgctt cttcaggttc cggcgtagaa ctctcgttct 39540
gaccaccagt agaggtctgg gtttcttcta cctttacctc ttcagctact tcttccggtt 39600
ctggttctac cggacgatgt ttagcttctt gcgggttctt acgagaacgg cgagtcttct 39660
tctctactcc cacgggtgga gtatcttcat gaacctcttc gttgatacca acggaggctg 39720
taccatctgc attgatgaca atattgatgt caccagcaag attgatttga tcatcgacat 39780
aagcctgtac agcagcttct acttcggact ggttcaaaat gatttgcata ttacgccttt 39840
aataacttaa tgaggttctg aaaagctggg agatttacac cagcatggat tgcaccgatt 39900
gcatcagcta agtgctcatt cttattcaga agtttgccct tcttatctgt gagccagttg 39960
gcttcaggat agatggcatg agcagcacga atcatcgtgt ccttagatgc agttttatca 40020
ccgaccagtg caagtttgtt ttcaattggg gatacttcaa agattggaca acccaatgca 40080
cgaaatgcac cgattaatcc tacgcataca ccataggatt tcataccatt agcagactga 40140
gagcctaccg gtacttctac gaatactgct ttagcagatc gtaaccattc ctcacaacca 40200
agaaacaaat catgggcggc ttgtatatct ttggagttgg ttcgaacttg tttgttgtgg 40260
tcaacttcag tttgaaccag tttaagttcg acctgctcga agacgccgga ctcaatgtcc 40320
agcatcccac gagctaaacc ccaattgcgg agactaatgt ctgcaccgac aattggtatt 40380
ttcattaacg tttaccgaac agtgatttac caccagcagg agtagtacct gtgccagcac 40440
ctgcacctgg tttaggaggc aagccacctt taccattacc accagcagaa cccttggtct 40500
tatcacgagt cttgcctttg ttcttttcca accatgctgc atagaataca gccagttctg 40560
gggtcagttc tttctcagcc ttctctgctt cctgagcctc gacgactgtg accagcagtt 40620
ccggatgaaa cactttctga atctcgttta cttcacgagt ttcgtcagag tccacataac 40680
cgttgtcacc tttaacctgc ttgttttcca aaactttttc cagagcgaag gtaacagtct 40740
ggcctaccag gtcaactgga accatgacgg atttattaac ttctgcttta gcatcgaagt 40800
catagacttt aacaatcttc tcttcgaagt cagcttcggg cagttcagta ccggtagcca 40860
tcagcatcat gtcattaatg acagtgtagc caggcaagaa gtattctttg ccattcttct 40920
cgtaagttgg cttgttacct ttagcattac cagaagtgaa gtacacctga gcacggaact 40980
caccagcagg tacgccatct gagtttttca ggtcttcaat aattaactgc atccagtctg 41040
caccagaatc agctttacct acgtaagcta cttttacagt accggtgtag atatcggatt 41100
cttttgcacc gaagccacca ccaccaaggg agtcttttac agcttcaacg tttttggttt 41160
tttctttcag attactgaac aatgacatat gattttccta ataagtgatt aacaaccagg 41220
caaacgctta agcgtagtat tcagccagat ggtctaagag tttctgggca tcgttatcaa 41280
tgtaagtctc agacttatcg aacataccca ttggggaacg aagtcgttta ccaacagact 41340
tcttggttgg acgggtctgg aatacgtgct tatagcctaa gtcacgttct tcttccgtaa 41400
tttctaacat cttgttccca tacttctcca gttctttaat gtctacacgc tctgcataaa 41460
ccacggtaga gaagtaggcc tcaagcccgt tattcttcag agagcctttc actgggatga 41520
atgttttcat cacaccggca gcttcatcca gttcgtcttt agcatgggca gtaatgatta 41580
ctggcttacc aaacttaacg actttttgtt gcagcagtat cttaaagaac tgtgcaaaat 41640
caccccatgc cttttgcgtg tttgcagaag gcagaacata ctgggattcc agcatatcca 41700
tcatgaaggt tgctgagtca atgatgatac catcaacatc gtctgccatt tctccaccag 41760
gagatgcaac atcaaatgct tcccagattt ggtaagggtc ttcaatgttg taggtattga 41820
acttgttacg gaaaggcaga cgtttacctg cttcagtatt cagatagagc cagcgttcct 41880
ggttccggat gttacgcagt gatgctgatt taccactcgc tgagaatcct gcaatcagga 41940
ttagctgagt gttcatatca ttgggaatta cttcggacat tatttttcct catttaattt 42000
aggagtcctt caaaccaaag aaagactcct gtttgcttac ttaccagcga agcgtttagc 42060
cacggtgacc attacggttg cattcaactc atcatctggt aatggattag ccagtttctt 42120
attgaatgag tggacggctt gctgtacttg ggcaaaatcc caaccactgt caaccaatgc 42180
cagtgcgtac ttaatcattt gattattacg attaccggta gcaatacgac tagcgaacca 42240
acgctccaga ttatccaacg actgtacttc cttcatctgg ttctggaact gttcgttctt 42300
actagtacga ggaataaagt cacgcacgtc caacagattt gcttcaagat tgtaatgata 42360
ggaaccagtc tcacaggaca tccatttctt ggctcgctgg ttagcagact catctgtttc 42420
gaacggtagc caagacataa cgttattcat gaactctttg tattcctcag tgtcgaggtg 42480
taactcatag ttcattggaa taatcagtcg gaagcggttc tcttcatcag aatgacgctt 42540
agtggtatag gtcatgaact tatattcctt catcagttca tggcacgtat gcagtggtac 42600
tccaccatca cagtcaataa caatcatgtt aaatcctgga ataacgtttt cttcagaacg 42660
atgcccattc ttcatgtgat ggttcaccca gtgcataccg ggagcttggg ttaatacatg 42720
tagctggtca aacggtacac gttcaccaac atagtcataa gcaaagctat cactatatgc 42780
cactatcatc tcattggtgt cagtctcttt tagagtctca ccacggaaga actcaatacc 42840
ctcattgaag gtcttcttaa taatgatgtg ctgtttgtaa ccccatgctg tagcaagagt 42900
catcatctca ttacgagctg cattaccact cttatagaac ggtaatgatt ccagcaaatc 42960
agcatgagta acttctttgc ctacggaggc aatgtactta gccagcttca cataggcttt 43020
ctcacgattg agaataccct ggaatgctgc cccggattcc tctacaagca agattgcttg 43080
tttaaggtgg gacatctcaa tgaatgaact ctggtcaaca aacgccagtg ctccagccag 43140
tttaagtgct ttgaagtaac ggtgggagat ttcagcttta cgaatttctt cgtggtcagc 43200
catagcttct gcttgtttct cacaatcaat tttgtaagta atcagagcaa tacccacagc 43260
atcttctaca accatcttaa agccaaacag gtttgggtca gccagactat ggaagtggtt 43320
agcccactta ccaagtgact gtacgttatc ctgcttaatc aggttacggt agatttcttc 43380
cggagacatt gttgcatgtg ctcgtttgtc taaatgccca atggcaaaca gacaacgtcg 43440
tgcataccct gtatcaagga agtcatagaa ctggtcttcg gtctgaccac catccagtaa 43500
cttacttggc gtaccaaata gcaacaggtt agctggagtt ttaccatcca gttcttcacc 43560
acgaacactt tcagcagtgt tcttggttag cttctgctta accttaccct ggtcgtataa 43620
ttctaggaac agagttaata catctgtgtt agccaacagg tttgaaccaa tttcatcaat 43680
ctgaaggttg attgaaccac acccagccat taacagctta tgccgtagtt gtttaactgc 43740
aggtggagta cctgagtcaa acgtaaacgg atatgccccg gcacgcttat attctgcttc 43800
aactttatca aactcatcat tctggtctgt accttgtcga gcagcacgtt cgttagcaat 43860
cttccataaa cggtcatttg caatgacggg catggtgtct tccataaagc gtttacggaa 43920
gccagtcatg aacccgtctt caataatatt taccgagtga cctttaccga agccagacgt 43980
tgctaatgcc atagcgtaaa tgttgactgg taagtcacca cggtctttag tgacaatggt 44040
tgcacccatg caggatgcca ttttagccag gaagtaggct acttcgacac ggaagaatcc 44100
tctgtcgttg ttttgtgtct tgttacacag cacgtctaca atttcttcaa ttgcagggtg 44160
gtgagtgact ccggtcaggt caatactcat gatggaaaat atctctctct ttgtttgcaa 44220
atggacgcga cagggcagta cagacaacgc ttaacctgcc cctccacgac cttgatagca 44280
cctttgccac ctttttcagc catgaagata cgagcagatg ccatatcgtc aaagtttttg 44340
gtacttctgg ctccaggtac atctacctta gaagcatcag agaagtattt gaactgtggc 44400
tctgttcgcc acaactcctc gtcagtacat tcaggaattt cctcttcagg tgcattccag 44460
tacttctcaa tgaggtgaat cttctcttct acccaacgtt cagtcttctc aacagatagc 44520
aacggaatat ccttatgcat aatacgatta gcaggatagt tcggattgct attagccata 44580
tgcttcatga agtcagtgaa gatgtagtta atacggatta catcctcggt aatgatgtcg 44640
ttgtgaatcc aacggtataa gctgccttgc atcttgtgtt cattatcacg ggaaccagca 44700
acccaggaat aggttgaggt agacttaaag tcctgcaaca gaccttctgt tacgatgtcg 44760
aacttaccac cgatagtcca acccttaact accctggttc cacgctgttc aatgtagatt 44820
gggataaggt cagggttagc atcaaagtct gctttggttg ggttaatgac tactgcatca 44880
attactcgct gaggataacc tagcttcttc aatgcagtct tatgacctag cttccaggct 44940
ttctcgatag agtcatgtaa gccagtaccc attgatgtgg atacgaaatc cataacatca 45000
atagactggt cactaaaatc cacacgatgc ttcatgacga tttgcttaat gggcttaagc 45060
aatgtagtag cagatagata cttaggattg tctacgtaat cataatcatc cgttacaagc 45120
catacagcca gggccagtga tacatcatgg ttgttggtta tcttcattag taagtaatcc 45180
tgtatctgtg aacccagttc cacttaattt cacttagctg attgaagtta tacgagtaaa 45240
tttcatctcg ttcatcttta taaacaatct caatgaatga aaattcttta gtgaagtgag 45300
gtttaccatt gtttgtatgg tgccaataac taccaacggt atttacattc gtagcaggat 45360
ccggaaataa ctcactagct aacttctcct tagcaagaat acgattagcg taatgaacca 45420
gcttctcaca gtcatacagg gctttgttac caggtttacc attacccata cgggcagcag 45480
catttcgcca tagagcttta aaggcacaac cttcatcgaa ggtcatgcca agtgcttgga 45540
taatatcctc acactctgct tgatatggct cttgctcttt ccgttgaggg tgcttaactg 45600
gtactacata ccagttattc aatccaccgg agagcttagc cattatttat ccttaggaat 45660
catgtttgcc tggaactctg gttcagacat cagacccagg ttgctaagag acaggtaaac 45720
aatgtctttc aagtcaaccg atgtgtcgaa acgttgatcc agactaatct tcagtgagtg 45780
attggcacgg gccaaatctt tataggtcac ataaggttca tcagtgagca acattgtgtt 45840
catggtaagc agggaaccat catccacttt agggagttgg aagactacct gtgctgctac 45900
catccaatga tattttctga ctacagtttc tttagtactt ttggtcatgc gaatgccttt 45960
tgaattattt gatgaacttc ttctggggta gcgtgatttg gaatttcaat ctcgttagcc 46020
cacgttgggt aaaacaagga tagttcccca cccaaaccaa cttctggatg agtaatatca 46080
ggatggtctt gccagttaac ggcttcaacc aagtgcttgt tcgtgaattg caaagtatcc 46140
atgttgtcac gaatcatgaa gtactgagca tcatgaatat gaatactagg acgaatatct 46200
aacctgaact cactggctct gactttacgc ataaactctg aaccagcacg gttatttagt 46260
agacaccagc tttgtcctaa agcattacca gcagttctgc cttctgcttc tgcttcatac 46320
ggagtcttac atgtcccacg taatacttga gccagtaaag gagtacgcac tctcaaaccg 46380
aatgcggcag taacataacc agttttggca gcttcgtcta gcttagcctg aacccacatg 46440
tcgctaacta catagaggtc gtgataacgt ttctctgtag tcttagcctt ctcttctgag 46500
aagccacagt ttttcattaa gcccatatat gtaccaccat aggtaagcag gaaggttgga 46560
gccttcgagt cactacgcaa tgatttgtac ttatgctgaa tggagttaat ggattctacc 46620
gtgtcttcta tgtccggcat ctgctcacca aagtaagcat atgcacgaag actgtgacca 46680
tcataaccat cggtatatac cttgagcttc tgagggtctt tagtagttag tgcagagata 46740
cggtcttcaa ggcttgcaaa gtccagtcca caaaagatcc aacccggtgg tgcttcgaag 46800
catttcttaa tcttcttggc gtatttggag ccagacggaa tggtttgcag gtttggctca 46860
gaggcagata atctaccaga aaccgttccc cccagattga ggttaccgaa caggtagtgc 46920
catccatctg gtcccggttg tgcattacgg aaagccggga tgaatgaagt gataatctta 46980
tccacgagct tatagtcgat aagtgcattg agcagttcct taacatcttc gctctgcgtg 47040
tgtgtacgca gtgctttaat agtgtcaccg tcagtagcag gttgcttgct cttagtaaga 47100
ccaagaaccg gtaagccaat atagtcaaat aatagcttct gtagttgtgg accggagtta 47160
ggattaaact cgatgtcaca atcagcaagc gttacctgtt tcttcttgag cttagcattc 47220
ttatcatcta cccattcctg acgaagcatc agggtgaagt tcttcatgag cttagactca 47280
cgaatagttt tcagagcctg gttcctgtca gtttccatct ccttagctac ttggttaacg 47340
gtatccatat tcatgggcat accggttaac tgcatctgga ttatgtcttc acatgctggt 47400
ttaaagatgt tgttgtaaac atctaattgg tcatcagcaa tgagagtatc ccagtgcttc 47460
tcataggtgt accaggtaca caaaccgtca atgaggttgt agcgtaacag ttgctcatta 47520
ggaataagac gaatgtcttt aatgtcatcc tgagcatagt taccagcata ctcctgagcc 47580
tggtctttta gactaaggtg attaccagca caactgttag tagccaggta ggtgattaac 47640
ttagtacagt cccagttgcg tagcatgatt tccataccat gcagtaagcc ttctgtatca 47700
atcaggttat ccatgaataa ctgatagata agcacataca cgtcgtagct aatgttgtga 47760
tacatctgcc gttgagtgta cttaatgaag aactcacgaa gaagatttcg aacaacatca 47820
tttctgttga tacgcccgta tggtgcttca gtagctccag gaatcggctc gtagtccaca 47880
ttaaaggcta tgccttgtgt cttactccaa cagaacgtaa ttgttcctat acctgcgtta 47940
tagtgcttta aaccgaatgc ctcgatgtct atagccagtg gcttattcat ctcaagcagt 48000
tggtctagcc aggctttaat cttctctggg gtatctggat aagcctcaaa ctcaatgatt 48060
ccctgacctg gttctgcata ctgcccacgt atgtggttaa gtaatgcatc cataccctga 48120
gcaatcttag atttcactac aggagggtca taaaagacct gtctgtagct aggtgcatag 48180
ataaccttct gcttacccca tactgaatca caaacgtagc cgaggttagc ctctgctttt 48240
gcttctttag tcagtatttt gaagtagtca gaatcggtac agataatgta ttgagtctct 48300
gcatcatcca gtacttgttg cagttcctgc tgtatgaact ccttaatctc ccttgcaggt 48360
gtcttctttt tgcctggggc agaatgaagc tcaatgaaga tgaaatcttc catgcttagg 48420
tcattaggca gcaggtaggt tttaatcatc tcctcccttc ggatttgatg accgaggata 48480
gcaataggat acttacccgt atcttgagag gtgatgtaac gcatagcttt accttagaaa 48540
atcagatggg aagctgcata gtactcaata gaaggcagaa tcttctcata ctgtttgatt 48600
gccatagcat caccagcaag tgtccaggct gcttcacgag tacgtggcag ttccttgtac 48660
ttaccagtct ggtcctgggc taccaggcat tcaggtaaag catcacgtac atcctgatag 48720
gacatacaac cacgaaccag gcgatataca gtctggttca caaggtgtac ttccataatc 48780
aggcgactgg aggctttcag atacttgtcc attgcaggcc aaagttctgg attaagtgtt 48840
aatcgttcac cgtaggttgg aggtctggca gcattagaac gctgatagaa ctccccttga 48900
taaagaaatc cagccgatat atcaatacca ttgatacggt cgttcttatc aatgataccg 48960
tttaacacac tatcaagacg aaccagttct gctaagaaca ggtgtttgac tatctcactg 49020
ctattggcaa cttgaccaat agtcgctgaa ccaatctgag gcatatatcc tcccttagaa 49080
agttagacca ccatacttac tggcaagatt gccatagaac actactcgct tacgggcacg 49140
agacacagcg acgtaaagca gacgggcaac catatcaggt tggcgacagc ttgagaggtc 49200
atctgcatca atgaagattg tgtcgtaagt agagccttgt gatttatgga cagtacatgc 49260
atgagtagca cgtaggtctg gaatggtttc tttaagacgg aagtaaggtt cccagttctt 49320
ctctttacct agccacttaa ccaaacggtt gaagtattct gggtcagtag gtactggaac 49380
ttcacttaca ataccaccat agccaaggtc cagagtacta tcacggactt ccagttccag 49440
gtcacctgta acctgaatca tgcgagtact gctatcctgg tcgatgagtt ttacgtcttg 49500
ctcgatagac agacggtctt ccacacctaa gcgaacagca gagttagaga ctagctgctc 49560
accgatggag tattccccca cgtagccgtt agcttcacga atgtagttat tgtaattaat 49620
aacctggtca tttgtgtaag caacaatacg gctgttggtg ggttgagtga agtgactgag 49680
aaccagtttc tccatctctt ctccctgtac ccagtcaata atgcctggaa tacacttaat 49740
aggcaggaaa cctgtcttac cttccaccgt atcacgcaat tgctggtgca atgctttaag 49800
ttccggtgca tcggtacgca tctgttcagt cagatagtgt gttgggatat tacctgcata 49860
cactggagac ttattctctt taacaggcag aagctgagaa gcatcgccta caaataccag 49920
tttgcactgg tgagtacctt cacgagcata cttaagcaac tgacggtcaa tcatggatgc 49980
ttcgtctacg aagatgattt tattcttctt aatattgaat gatttagatg gtacgacgtt 50040
agcctcacct gtcttaaagt cattcttaac aatcagtcct tggaaggaat gataggtaga 50100
cgttggacgt ccagtagcct gagccagaac ttcagcagct ttgttcgtgg tcgcagtcat 50160
aacaacttcg ttatacagag gcttggttcc cataagggag caagtttcca tatatgcagg 50220
catagtgtca tcaatgaggt gcgacatcag gaatgtctta ccagtacctc ctgggccaga 50280
aattccaaat tcggtagcat tagggttgag caggaagtcg aagaactcct tcgcaaccgc 50340
ttcttgcccc ttgttcagtg gggctttggt aggtttggaa aacattaaaa tctccagtaa 50400
aaaaccctcc gtagagggtt agatgtaagg ggttaaatct gggggagtgt agtctttccc 50460
tttcataact ttcttattct cattgaagac aggttcacca tcttcaaact tagaatagtt 50520
tgagcgattg acctcagcca atgctccagg gacattcatc ccaaggaaag taccgacgcc 50580
agttgctgta acaatctggt ctgccaggga atccagtagt tctactgaat ctttgataac 50640
aatattgtta tcctttttaa gcagattacc cacatggtca attgctgccc gtaatgcatc 50700
aatttgtgag gcacgttcca ggttcttact ggtaatagcg ttaagcattt ctgctacttc 50760
ttccagatga caaccaattt gggtactaat gtttttactc gttggatttg gtactgcttt 50820
ccggaaccag gcttccgtat ttgccagcgt acaaacggat gaacttgtca tgctcactct 50880
cttggttaag gtggcgatta ttatatgcct ccagaatcaa gtctcttaca tatgagcgaa 50940
tctcataagc aggaccaact tgttccgata gccaatcagc cacatgggtt ggtaaaggac 51000
gtaatacgtt ttccatgaac gtccgacgtg tgtcggctgg ttcaatgttt agagaacgca 51060
atcgcaaagt aaccgtagtg gggtgaaccc ccaacgtctt agcgatagtt gcgagggaca 51120
gaccaacagc attcatacga atgatgtctt catcagttgc tttgcgatta cttctgaata 51180
ctttagacat agcttatttc ctcttcaaag atagcctcac tttattataa gtgaggctat 51240
ttgaataggc tattacttag cagctttgac cagcttggta gctaacatgg cttcttcttc 51300
agtcagaccc attgcagtga atgacttctt agcatggcgc ttatagtctt gcagagcatg 51360
gcctttctga tagtcagagt acgtcgtatc gttaaggatg cttcgcagat tatcagcgtg 51420
tttaccagac ggagctactt tagtggcagc agcagaacgc gtaggtttaa ctgtcttatc 51480
ctgcttaaca gctttaggtt taccaatggg cttagctgta taggtagcac gtacatcagc 51540
aatcttagct actacatcat tacccacatc gaatgcagcc caaggcttac gagctttacc 51600
acgcagttct accagaaggt cagcagcagc taacttatct tctggagttg ttgcattgag 51660
gaactcttta accagttcct gaatttgttc tttcacagta ggagccttct tactaacagg 51720
tttaaggtca gtagccttca ctggttcagc agctacagct tcatttagtg actcttccat 51780
gttcagtgct tctttactag caactgtacg gaattgttct acagaaccag atgagagatt 51840
ggtgtatttg aggttagtgc cacgatgacc accaaccata ataagtgtgg taggtgaact 51900
atggttccca acgatggcat cattaagcag tttcttacct acttctgtct tagacaggta 51960
gataccattg ttaacattat tacgttcctc ttccggtagt tcagcaataa tatgatacgc 52020
agatacacgc atcttattgg tgttgtactg aggaacagca aatacatctt caggtgctac 52080
tttaccaagg atagttacgt caccaccgaa gctacggata tagctcatgg atgctacgtg 52140
cagaccctta gagcagtcct gacgacggtc agggtctacc aggtcttcac gtacctgtac 52200
tttacagcct acccactgac ggatattgcc tgaatgacag tcaacgaata cacgtttacc 52260
atcctctgtg cctttatact tcaggcgttt caggaacagg atggaaccat catcagcaat 52320
aggtagttca gcagtttcca tgaacttcat caggtcttct actgagtgca gacggtcttt 52380
aataaccgga gccagacgct caaggaactt agtaaagccc ttgtagtctt tcagctgggc 52440
agactgacgc aagtgacgat gtaggttctc aactccgggg ataaccccat tgttggtaac 52500
agcaacaacc acttcgtctt ccttcacttc agtgtggaag ttagcatcat cagtactgat 52560
agcacccaat gcagctaact tctcagaagc agcagccagc ttatcctggt tcgtagtttt 52620
agctacttct acgtaaggaa atggcgttac atcacggctg gtgaatacaa tatgtacacc 52680
tttgatgtgc ttggcttgtt tagcaaactc acccatctca tcatcggata ctgcacgaat 52740
tgcaataggc catccctgtg caaggataca tctggtatgg gcatatccac aacgcagtac 52800
ttcattgata aagcgatcca gtgcaggatg gttgtggttt ttatcccagc cagtaaccca 52860
catcagattg tcacgtttgt cataatagcc gtccgatacc gttacttctg gttcattagc 52920
ctgtacagcc aggaaggtat ccatagcctt agacactaat gttttggttg gattgcctag 52980
cttaatatca gaaatgatgc ggtcaggttg agtacctgta ccatcctgga agaactcttt 53040
aagtttagcc ttagctacct tgaagaaacg gacgaatccg ttagtgttct tctctgcttc 53100
cagatattca gtacgcaggt ttacttctgg tgcaatgttt acttctatct ggtctttggt 53160
agttcccaga cctttggttt gtgcttcaga tacgatgcga gcaacccgtg ggtcgctctg 53220
aggatatata atcgtggagc catcaggttt ccacagtgtg agattgcgac tgtcaactgc 53280
cagtgcgatg atagaaatca gttcgatttt catcagatta gttacctttc agtttattta 53340
gacgattacg gtagagggac ttaaacccag gaatatcctc tggattcttc ttaaggtgtt 53400
taactaaact acctaagtcc agcgtctgta ggatgtcatt tttattccag ctataactga 53460
ataattcttt gtaatcatta agtattttaa agtacttatg attacgcgga ttagtcatta 53520
ttgcatatgg ctctaagtca tccactgact taatacaacc catttcaaca agtttttctt 53580
tgttttcatg tggtcgattc cgtagaaagt catatgccca ttcatactct ggtttaaaaa 53640
tcagattctg taatggtttg aatgttagac ccagtatgtc tagaatttcc cagtattctc 53700
tgtcatccag ttctatatat tccagaatac cgatgcgatg ttcagtaaca tatttcttga 53760
atcctttgct ggtaataaca gacatcagtt ccataaagcg aacatcatca atgtggatag 53820
caccacgtcg tttggccttg ttagcttcta tttgattacg gcagacaaca gtctctgctt 53880
tcatctcatc agataactca taccatttcc acatggttcc aattcgagca gtcacaggac 53940
taccagaact aatctgatta gccagtacgt agtacttagg ttgctccact tcgacgaaac 54000
cgtacttctc atggcgactt gggtcagcaa attccttctg gatatatggc ttgggttccc 54060
aatcaccatt tttataccgt acccgggtag tatcaataac tgcattcaat gaaaccagtg 54120
cattagtttt acctcctgct ttggttttat tggcagctat cgttttagcc cgtttctcag 54180
cagctatctt agcttgttct ctgcggaagt tagttggttt gtcccactca ttatattgag 54240
taaggtcaat tacccgataa cccatagcag tgaacttagc tacagcttct tctgctgcac 54300
ccttcttagg accaacgacg tgaacaaaag ctgtacgctc cagatcacct cgattgtact 54360
cagggaagta tgaaaaagag tctgcacaat cagataaacg gcgagtaact acaacgttct 54420
tggtagtaaa tatgccaata ccctgatggg agttatgata catcttaaag tagtcagtaa 54480
gtctaccctt caccaggtca acacgattac cttggttcat tatgtaacca gaccacttaa 54540
taccagaggt tttcaaagca gcaagctgtg gtaagtgtag atggcgatat actaattcct 54600
ggaatggtga gtatgtacct tcttcacgag tatccttaag agcacgcata gccttaccat 54660
acagcttctt aagacctgta tattcagggc gagctaacaa agccttattg gatagattac 54720
gccagtgttt gatatgatgc ttacgctgct tagcccacaa gctagaggac atatagcgtt 54780
gaacagttct gtctgtaaca gcaagtagga aagatggata ttcccaaaag cgagtagagg 54840
atttagaggc atattcttca acctgcttaa tagcttcagg aatcttagcc ttaatctctt 54900
tctccatacg gtctactaaa cctacgcata gagtagtaat accgtcgtct gttaacttct 54960
ggttagacag cgtttcacga ctaggagcaa tagctagagt atccggtgca gcctgaacta 55020
ctaaattgtc agcaccaatg atattcataa aattgaggat aagacctaca gcctcttcac 55080
tagctgggct ggatacaatt gggtacatta cgttaccgta acgaacgaat atgttactgt 55140
tgcccatata gcctttatac cagctatcag acatatcata tgaaccaggt tcaaatgaca 55200
tacctagtgt actcagtagg gtgtagtcac cttgttgaat attattacct tcttccgttt 55260
taacgagttt agggatacta agctcggctt taatctcacc gttaaatacg attgatgtga 55320
tgtaatgaat aaaggtattt aaatcatgtt tgcctaactg gaatttaact tccagaccag 55380
attcctcagt aggaatacca gtaacaatag gaacaatgcc cggcttaccg tcattctcaa 55440
tcgcagcttt ggctacgtta tacactgaca tctttccctg gttccacgaa gtaacctgga 55500
agctatctgt ataagcgaat ggggatttac atcccagacc aaagccacca gtaacactac 55560
tgttggactt cttagtagat gcaccataga caccataaat ggaaccaatg agttcatccg 55620
ggattccatt gccgtagtca cggaaggtaa taaagttgtc tgtatcaatg gtgatacgaa 55680
taggagtatc agtcttaccc gcttcaatat gagcatccca actgttacag atagtctcac 55740
ggactactgc taaagtagga ttgttataca gggaagtact taatacatga aagaatgccg 55800
gatcatcaga gatgccaaaa gcaatcgttt ctttgccacc cagtgtcgct gaggtagcta 55860
tttcattggt atctgcaact aacatttatt tactctccag attaaatgtt gcttacatta 55920
gaacttgttt acacttatca aaaatagctt tacgaagttc agcttcttcc cgtggttcat 55980
cattaggatg gtgacccact ggactgtttt gaaaagcatt cattaggaat aagacttgat 56040
actcggttaa accattaatt tgaatgctat acgttttctc tgaggcgata tttacattag 56100
ccattgcgtt tacctgccat tgcttgaagg acaaacacgg ccatatctga tgccatgtca 56160
tccgttatat cgttgaccgt cccataaggg agtgggccgt tctcttccca gttatctaac 56220
gtgttttgca cttcagctaa accagcctga atcatttcct tgcttggttc taaccataac 56280
ttacgctctt ctgccagtgc agcaatgcac atacataagg aagcaatatt tgctggacaa 56340
gcagcaatat caccagttac tgcaatctca ctaagtggga cagccttacc ggttttacta 56400
tgaattactt caggattaat catgaatttg ctccaggtta tgggcaggaa tagggaagca 56460
tggatagtca ccggtagggt ccagtgttac tgacacatgc agtgtatttg tcatttgggt 56520
tagcatctga gcagcattga aatcatcctg tatacaggca tatacaatgt cacccttctc 56580
accctcacac attacaggtg tgtctttaat acggtattta gcaggcataa accctcctaa 56640
aagagttagt tgaaaccaaa aaaagacctc ccggaggagg tcttaggttt acttactatt 56700
ttcttcaatg aagcgtctca tacgttcctt tgcttcatcg tgttctcgaa ttgcttcata 56760
gtcgcgagca atataataaa gacaccacaa accaatctca cttacaaaga acagaatact 56820
ggctcgaatg aactgcatat ccgtttctac tacaaaggcg actttgtatg cgtaggtcag 56880
tgttagtaag gtactaatca gcattgcgta cagagctagt tttttcattc agtaatagtc 56940
tcaccaggaa ttaacttacg gaattggata aagtgaagca ggttacctga gtacagattc 57000
ccttctttat ccatggaggt aacaccttgt tcccaggtaa ctgggttcat gttattcaca 57060
tacttctcac caaggttcat aggcgtagcc tgatgttccg ttggagaagc atgaataggt 57120
tggttttcca ctaacttagc aaataagcca aggtcttctt cgatagtaga tgggcgtcct 57180
tcaaagttgt tatatgaagc acgagcacaa cgtgcagcac ttaccttgag aagtaagcta 57240
tacacctctg tactacttgg ctcatcacga gtaattcgcc gatgtttgca gaaattgtaa 57300
gcagcactac gatcaggaaa gccaatgtaa ggtaaatgcc actcaccctg ggacagctta 57360
gatggaacac tttcattatg tgccttacgc atggctctag ccagttcacg aatctctggt 57420
tgtgcatctt tgtggtcacg cagtccatag aaattagccc agcttgttga agttaccact 57480
acacggatat gtgtgaatgg ttccagaata cggttaacaa tctgcttatg tacttgccca 57540
cgacgtaact gttcagcata cacagcagca gcagatgcag cgttatccca gataaactta 57600
gcatcctcaa tctccatagg agtgagttct tcatctgctt gcataccttt ctggttcttg 57660
ccccagtgac ttggcatcac tggctcatta cgaacctgtt cgattaactt agaggtaggg 57720
atagcacggc tacttgaagc attacggttg aatacacggt gagtcataaa ctcactgtga 57780
atgaatcggg gataaaccaa ttcaaaagtt gtgatacgag ttcctgtttc tggatgaacg 57840
gaatctgcaa taactgatgc cttaatcatt agaatgacct tagatagttg gtgataccac 57900
ttctcgtctc tgtgatcctc ttaacctgaa cataaaccag cttaaactca ctacgaggta 57960
gttcttgtaa tactgctaca ccttcagtaa gagccacaga acacgctaca gcggtttctt 58020
ctttaaagga gtacaagtct ccatacatga gggcttcata ctctttacgg agcatacaga 58080
gagcatgtct tgctttatcg cttacttctt gtgaaccagc agacatagct aagctgtata 58140
tgataaagac ttgttcaaaa acaaagtcca ataatctatc agggtttcta gtccctatga 58200
ataattctgt tgtgtaggaa cgagctttac gattgagttc atactcatcc tctatgcctt 58260
tattgtaaag aacaggattt cgttcccttt ttcttaggaa tgggatacgc attagtattt 58320
actacctctt ctataccagg ggtagtggtg agtagtgctc cttcctttga atacatcctt 58380
ggtataagga ttcttttgta tttgagccaa gtaaagatgc atttcgtcat aacacggact 58440
aagccagaac ttatctatga ttcggtctac cttagatttc agatgtggtt ctggtggaat 58500
atatgaagtg gtgttaaaag gtctactagt agtccatcct ttctgaataa gaacctgcat 58560
tgctgcataa acatcagagt taattccatt cttttccact aggtcgtgca tagttttacc 58620
taaagtaagc attgcagttt gttctagttt acttaagtcc agcccataca agatagactt 58680
accatttcgt ctgtttgaca taagagtacc tgtaattaaa aaagcccacg ggttaggtgg 58740
gcttgggtta ggagatggat aagcaatgag ctggaagtaa cttccagaag taccctctta 58800
tggatacttc ggttagtcac tctgctttac tgccaccatt ggtttgaaca acagttacat 58860
ttgtaacctg gttctgacct ttgttggaca gtgaccaaat cagtgctact acccagaata 58920
ccagtgacca gcctaatacg atattaagca caccaatacc ccacttagag gcatggcctc 58980
gtgcaaaagc aatgatagta gggataaggt atacagctaa tataaacaac ataacaagga 59040
ttgcagctaa agattccatg atggttccta tgtgaggttg agttagtttc ttcacaaata 59100
gcccagctat gttgggctat ttaggcagtt acttactcag catctttgct tgcaggagca 59160
agagctttct tcaggtaatt acgtaccgaa cgataggttt ctttggtgtc catagtacta 59220
tcatttacga atgtgatacg tgcacaatca gacagtggaa tttgttccac tcctactaca 59280
tttgcagcat taatggttac acgtacatct gaggttgcat gagttacttg aaatactgac 59340
atgttgtttc cttatttact gcttcttgca gctactggtt tgttttcctg gttcatatac 59400
ttgccattgt aattagcagg ttcttgaacc agatgaaata atacttgggc tatcccagca 59460
ccagccggga tatgcaattt cttacgacca tgatagacca gttcaagggt taagtaccct 59520
ttccatcctg gttctattac ggtattgaac actgataatg cacgtcttgc ccaggtagac 59580
ttatcgtgaa cgatagctac acaggatggg gacatgttga acttctcaat tgctgaagcc 59640
aatgtgaact taccaaaatg tcgtgatact tgctttccat caacgacctt caccattgga 59700
atcaacccaa ataagcgata gaaggtaata tcttgtttga tacgaatatc ataaccagct 59760
tcacctaatc catagcttac accatgttcg gatatcttac ggtcagggac atccagcaac 59820
tgagatgaac gatatagaga attaccatta acaaccatta tttcattcct ttagggatat 59880
gaactacctc cccaaaaggt acggtagcat tgggattatt cagaacacac cagatgatag 59940
gaatatcaaa tggcagttct tccattggac gaacatacat atcggagaat atgattgcag 60000
cggttggctt attcttaata atccactcgc gtacaggtac aaggcttgta ccaccacgac 60060
ctgtaatctt aatctcttcg aaagtatctt cttcagtgat gtctatctct tcttggataa 60120
tatcgtcgaa ggtaattaag gacatcttct taggattgaa ctggctctta acgtaggcaa 60180
cctctgagtt aaaacgcaga gagtcagcac tactaataga accagataca tcctcaaagt 60240
aggctaagtg tcgtagacgt ccttcatcat cttccagtga aggtagataa atgtcagtgt 60300
aacgacggtt aggtctggcc cacgtatagt gtgtgtcatc aatgtcggta aagaagttca 60360
tcagaacttc ctgccaagga accactggct taaggaattg ggtaataacc tcttccatgc 60420
gtccagcacc tttaccaggc atctgtccac cagccatctt ctggctctgc attgcacgta 60480
ctacgttgtt gacgacagca ccctgggact gccccgaagt gggggatttc atgtcgccag 60540
caagacctgc tccagcaccc cccgacggct tctgcctttt ctgagggttc ttcatcaggt 60600
catcgtagat gtcttcttcg acccaatcct tatattgagg gtctttgcaa ataccttcgt 60660
cgataccagt gaacttgtaa ccatcttctt ctaactgaag gttaatgaag tggtcacatg 60720
cctggttcca gacttctggg tcacggcttc cacgacggat gtcatgtaga tacgccacat 60780
gccagagttc gtgcattaat actgtttctc ttgcgtctgg acacatccag ataaagaaat 60840
ctgggttgaa atggagttct atcccatcag tacatgcagt gggacaatcc tcacgtttcc 60900
atgagaattt aagactacac agtaatgaac caaagaacgc agcagacttg cccaggaagg 60960
cttgggcctg aatacggtca tactcacgca tgagttgttc atcactaagt gtgtgctgag 61020
gaaattggtt cattgtttta cccgtgaatg tagcgagaga tattagcagc agccttacgc 61080
cattcagggg tagcctgaag atctggtaat gcattgccta ctgtacggaa gtacagaatc 61140
ttatgagtga atgttttaaa gcgttcaata tactgaagaa catcagcaaa gttttcttca 61200
gtagtcttgt tagccagatg gttaacggta gcccaacaca ggttgttatc ttctggtagt 61260
gcacaaccca tagggtcttt gactactttt tcaatcgtaa taatacgatt gtatacctga 61320
gtaaactgaa caaattctgt agccttacca gcagtgacgt gacctgcata gtagatagaa 61380
tcttcatcag gcagagcacc ttctggtaag ttcttgaggt ctttattaac aaagtcccaa 61440
gtacgaggac aacagaacgt tttgttctta tgtgctgggt cgaagtcatg caggtaacca 61500
gggttagcat gtaagaatgc aaccagacgt tcatcccatt gttgaggaat cattacatct 61560
tcaacaaaga tgtcgaagtt aagttccatt tcaaagtgaa ctacacgaga ctgcattgca 61620
gttcccagtg ggttaacaat ggcacggtca gtagctttgt taccagcaca gacaatcatc 61680
acattaggat gaagtttttt ctgaccagtc atacggtcca gaatcagttt gtatgcggca 61740
gctactacct ctttcttggc tgagttgaac tcatcaagga acaagagcca gccattatac 61800
ccttctggta attcatcccc ctcaataggg aataagtcag cgaatggaat aaattctgca 61860
cggtcaccat tacggaatgg cagaccagac aggtcttccg gggcagaagt agacagacga 61920
tggtctatca gcttcatacc aaattcttct gcaatagaac gaatgattgc agatttaccc 61980
atgcctggcg atgaagtcag gaacggtacg ttacctgctt tcaaggcacg gataataaag 62040
ttacgggctt gacgtgaatt acatacggtc aaggagtcaa tgcttgccat ttacttactc 62100
tcctaaagct aatggaatta ctgattaaga tttaatacat gggtatgctg tatcaattgc 62160
taacagaacc atgtctgatg gtacttttac tgcattaacc tttcggtcat acagaataat 62220
agtggctact ttgtcaaaga catttcctaa tttcaaatgc tcgggtacgc agttttttcc 62280
tgcttctaca cccatggtaa ccattcccat gaaataaccc tggttcacaa agtttgcatt 62340
attggttttg tagtcagcag cagattccaa cagagtttca cgacttgatg cttgagcatt 62400
gaatgacaga actgataagg ctactacagc aacagcaatt aaagttttca tttagttacc 62460
ttttaattcg ttgagttgaa gtacaccggt aggggttaat gaccacccaa gaccagtatt 62520
gaactgcact aagcccagtt tcttaagaga ctgtgcggtt ttgttattac ggttgctggt 62580
ggcagaacca ccttgaacca tacgaaggaa gattgcttgg gactgggtta atttcaaagg 62640
taatatcctt ccaggtttat tggtgttacg aatgtacttg ctgatagaca gaaggattta 62700
ccagtctcta cagcaacagc agcaggttta ccatcaggta agtgaatctt tagacatact 62760
gtttggttat agataaaacc attggcaggt tgcacatcac cgaaagttac ttcattactt 62820
tcagaacgat ttgttacttt cactcttctt ctccttcatc tgggagtggt gataaatcta 62880
cacctaaatg ctcaaaacgt ttctctaaaa cagcaatcct cttttgataa aatgacagca 62940
gttcaggaag tattcttgta cgggcttgta gtcctaaatc cgtaccattg aatgatactt 63000
tcaccatatg tggtgacttc ttcaaactag ctaattgctg tcggttgtcg ttgtattctt 63060
catacagttt ttcaaccaaa cgtagattat gaatattcat ttcttctccg gtacgaactg 63120
cattacacag ttttgattac gagggatatt caattcacat tcatgcttga gggtttgtgt 63180
tacagaattt ggagtagaac caaagtaggt attagaaata gctacaaatc ctgcacctat 63240
caaaccacct agtaagaatg agaaaaacgc aatctctttg tacatttcct ggctccagga 63300
tgtttaagtc aaaaaaaacc tcccttcaat agggaggtca aacttgaggg tttcatttca 63360
gggtttataa ccccaaataa agcaatcata cttacttgat tagataattc agatattctt 63420
ctgggtcatc aaagaaatcg tcttcagttt caatatacca atctacccaa cggtcctcca 63480
attccctatc aataaggtct tggtcagtga gggactcatc ccacatctca ataccgttag 63540
cattagcata atcgcctttg acattgttct catactggaa taggtcatag tctgctaacg 63600
cattcttaat cttaacagct tcctgaagac tatcagtctc tacatgaaat gccttcattg 63660
gcacttgggg aatgtgccat acacgtaatt tctgtgtcat ttttactctc ctatagatac 63720
aagaaagccc tcactaagga gggctataac cacaaaaaga agtactctta ttctgcaaca 63780
gtaacaggga tggttacatc gactgtaccg ttggtaacta ctacagaagt agtatcacca 63840
gcagccactg gtttaactgt cagtacgtta ccagcaatgg tagccgtagc acgagcagaa 63900
tctggagcag ttttgataga caaagtacca gcagatgaac caacgggcat agcaacaatt 63960
gttacagtct taccagcaac agcatcggca gcaacagaca gagccagtga agcaggagta 64020
gctaccagag agcttgcttc gtagtatcct acagccagca gcgactcatc ttctaccgtg 64080
gttccattag catcagtaac aatcacttta taagtaccag cagaagcagc tgttacgtca 64140
ttaagggtca atgttgcagt aatagcagat gcgttctcac ctgaatcaat atcagcgaat 64200
gctgcaccag cagtatttgc tttgaactgc catttataag taaatggtgc tttaccacct 64260
gctacatcaa catgccagtt aatatcttca ccttcgatgg cggagactac acgaggcagt 64320
ttggtagcaa tagtcagtcg tggagtagct ttcatatcaa tagagataga ctgcatatcc 64380
acgatgttgt taggccagaa gccttcttta ctagggtctt tagcagaacg tttatacagc 64440
aggtcacgaa caccatgata gataactaag ctatcagggt aagtggcatc tgggtgctca 64500
aaggttccta cttctacgga accacccggg atagaaccac tggcatccag tacagttgca 64560
acattagtgg ttttgttaaa acctactttc aaagttggca tatcaatttc ctcataagta 64620
aatagcagga ttagtataca tgacatgtaa aaaaataaac ccacctagtg gtgggttagt 64680
tgtttacaca gtagatagca aaagctatcc atcctgccat tacaaagcag cctagcgagt 64740
atgctccata acagtagcaa gcaaacttag atttgtgggc atgaactatt gtagacagaa 64800
ccagtacaga taaaactatc agtattaact gagagattac ttcaaaaggg attagcatag 64860
ttgttacctc caggcaaaaa atatgcctca ctcccgaagg aatgaggcta gtttattaac 64920
tcaacgcata gtctgtttca agaatgtcct gccagaggct gtcatccagc ttaccggcag 64980
agaactcttg tcccagtacc tgagacagga tgaagcccaa taggtcactc ttagcaatat 65040
cagacagaat ctgattgtac tgacgacgta agtcattacc gtagttagga tggcaacgga 65100
agcagtcatg tacagtcatt acagggaatg gtttcttagg cagagtgtca atcatatctg 65160
ctattacctg acggtctacc aatgcaatgg tattcgggtc aaggtagtcc agaatagaca 65220
gtgacaggaa tccagacttc tcataatgac cccatatttc catagtcatt tcctggtttc 65280
ctgcgatagc aggataattt cttggcccga tatcacatag ttcacgtaca taggcaacca 65340
gtttagggtc gaagttacaa cgacgcagca tctcacgtac caccattcca tcaatagagt 65400
gggtgatgtt agcagatagc atacgagtct tctcttcagt accctgaacc atacgagtaa 65460
tgtcgaaagg cttacctaag aagttaacag tctgtacttc aggaaccatg accttaatga 65520
cagcatggaa gttgtcaggg agtacccata cgtaacggtt ggcatccggg ttaccgcatt 65580
gcagtaagaa cttgttaagt gcccaaggac cactagcaac agtttccatt acattctcaa 65640
aagtcttgag acgaatacct tcaccgaata cttctttagg cttggcttct gagccgtaga 65700
acgcagtcat aactgcctgc ttagtgtcac tacgtttaat acgagatgct tcaccaagga 65760
tgtccaacat cttgtgatag atgactgtat aagcatcacg acgtaatggc ttaccattct 65820
ctcccatgta gttaactacg ttacacagtt cagcagcaga gcgatcacca gtcaggcaag 65880
ccagtaactg caatccggat gatgtagcat ccagggctac ggcataacca ataggcttac 65940
ctgctttcat atctttccat gcatttatac ctgcaaagaa cagagcagaa tcttcagctt 66000
catctaccag attaagcagg ttagtttcat tttcatcgaa ccaggcaata cgctcatccc 66060
atgtcttttt atccaagcca tagttattag caatatctac tttcaggtat tcacgagcgg 66120
ttaacagttg cattttatgt tccttttgct tcttggctcc ccactatgga gagcctgctt 66180
attgggatta ttacttccag gcccacttaa agcctttgta tgttttgggg ttataaatat 66240
gacctctgcc atcctttaca gggatgccgt tggctactgt tataataccc cacgttctac 66300
cacttacatg gcgtgcagca tctgctgtag tagcaaattc tttatagaat gtgccatcta 66360
ggtgataagc gataactggt tgtgcagtgg caatgttacc ttctttctta ctacgtgctc 66420
ctttatccct attctctttc caggacatta gttgaatgtt atctagagta tatggtttgt 66480
ggtcatctat acggtctaca gatggtttgt agtcttttgc atagttactc gccacataat 66540
catcccatag ttgatggata tttggttgct tatccaacca ttcatagaaa tcttctagtg 66600
aatagctggg tggtgggtgt cctcttttaa tagagtgaga ttgttgagtt agataagtct 66660
tcctgaacat acatacaggg tcggctttgt tataccttaa ggtccggcat ttacggcacc 66720
gtacctcata accgtctttg caggttgatt ttggtacgaa ctctgtgagg tcttttgttt 66780
ccccacagtc cttgcagatt ttcattagtc ctccacaagc tctttatctg cgaactctac 66840
tactgcctta ttccatgaag tgcctgcata attaatgtgg tagccacagg catatgtacg 66900
cccacgtttg tcatatttgt gagccagtga gaagttatta ccttctttgg tcactagctc 66960
cattacttct ttagctgtac ggctgtactt ctcaaaggct ttaacacgtt tctcatagtc 67020
ctgacgggtt tcaccctctt tgggtttatc catgttagga cgagagtctt ttacatagtt 67080
agcaacgtcc cagttaatgc agaggcgttg gttattcatg cggttgatat ggtcaagaca 67140
gacatccatc tcatgatggt tcttcttgag gatgatgcta cctctgcttt cgtagtaacc 67200
agtatcacgg ttacacttaa caggcttagg agcacttacg ataggcaatg ggtactggaa 67260
tgcttccagt tccatctgta catcttcact aatctcatag ataacaccga acttgtcgat 67320
ggtagggtca tagttgaagc agtcattctc tgccatctta tacagacagt cagcgatgta 67380
ctgagcatca tctgaatgac tcatgagaga gccaatcatg gtctgaatgt cagcacgttt 67440
atgtagggca atctgcacca ttgcatcaat gagcagctta taatcaattt ctaaataggc 67500
agcgaatgcc ttaaagtcaa tttcttcgga gtcctcgaat tccttcctca tgcgaggtaa 67560
gagctgattt ttattgaata gtttttcgag ttgcttctga tgttcaattg atgagtacat 67620
cgcttattct cctaattaat ttaaacaata cttgctatta gagacctggt tttatgctca 67680
ctagcacatc ggtaatacat tcttaagtag tttccatgga agttatgtct ccatgcccac 67740
gggaaggata attctacttc acaccaatta ttagggcttt catatgcccc tttaactgct 67800
tctttaatct ttggataatt accccattta tgtttatgtt tcttagctat gatctgctga 67860
attgatactg acatttcata tccttaagct gcggagcagc tattacctaa cgcttagtcc 67920
aaaaggtaat acgaatacta ttttcattac cgatgtagtc gatgcgtaca tgcaatccat 67980
taccattaaa ttcattactt ttcttctgat aaggccagac taggctagtc ataggtattt 68040
taactaaacc agatatgtga cccagcttag tacgactctt taattcgttc tgtaatgcac 68100
aatagatgtc atgtcgtgtg agtgctctgg ctctgacttg agcagaccat aattcttcat 68160
aagtcattat tagctccagt agttgtaagc atcacctaag tgtttacgcc atgcttctgg 68220
taacagtacg ttgccagtaa cattgataaa ctggtatggg tccaaatagt cattactcat 68280
aactttgaaa tcccattgtg gattagaaga tgcataggca atgaaacctt caatgaagcc 68340
atagatagta ccctctttaa tccggttacc ataaccatcc tttactggaa tggcaaagct 68400
attaccataa tgaccatagg ccatacccca gcgagcaccg tgcttcttat atgctaattt 68460
ggcagcagca ttgatatgct gcccaagtac attggtagtg aatactaaga tttctttcat 68520
ttacatgcct ctctgttata aatataagca tcgtgtgtgg tcatattgtt gttggctctg 68580
gttaattcta agtaagtaag agcatattca tggtcacgtt gttctttctt ggttcggcaa 68640
tgaccacatt tactatccca ttgggataca tggcctttac cacatttgca ttgtttgttc 68700
atttaaaagt actccatggt atggactcca agtaattaat tttgtaaata atcttacttc 68760
ttatttctcc accaacatct tgtacatgga ttaggtttaa cctttgtgct tgggttatag 68820
tagctgcaag agttctcccc ataatatttg atatttctat aaaagattta ttggattcta 68880
ctagtatctt taaattagta ttatctaagt tactccactc tttagaatga tttgctggct 68940
tatccattag gattctccac aaaatagtta tacatagctt ttactagttt ctctttaata 69000
atctcaccat gacaaggttt cggataacag aagcactgta aggctaaccc tttttcgtca 69060
atggctttat tacccagacg attaagttca tccagtacaa ctggattctt tctcatgatt 69120
tgttcattaa gccatactcg atatttggct ataacttgtt ctcttgaacc gtctttacca 69180
ataacgaatg gattacctaa tggtgagcca cgtccaatat actcacctga tttaccgtga 69240
tgtttattaa caatgataat tggacgtgta gatacaccaa gtaaagcatt ggtgtcgtca 69300
cctaatgaca taatcttcat ttgttactct ctctgaatga tgtcatatca aataaagtag 69360
cactatttag tatagtacca gctcctctct ttgatctttc tgaggcaata tctagtctat 69420
gggcactgca ttgaattact attacgtact ccttaccttc atgtttgaat ttaagttctt 69480
tatgtaggta gtcacttcgt gtctttttag tcttcatggt ttaagtacct gaaatgggaa 69540
cacaggacgt ttactacgcc actggttcat agctaattca cttgtttcag aaccatcatg 69600
taagtcatag ataaatgaga ttacatagtc tggttcttcc gggaagttct ctatagagaa 69660
tggtagacct gattccttaa ggataggaac caatggagat tcagcatcgc agtacagagt 69720
aaactcttca ttgaatgcct ggcttgtctg agatacataa tcaatattac gcatcatctt 69780
atctacatca gtaaatgtag aatcatgaac tactagaata gttttcatac ctttctcaat 69840
gtcccataga tgttattatt gacaatactc aaatcacaga aaccaatctc ttcttgtctt 69900
ccagaggata cccagatatt gttttctgtt agtttaggtt ttttagagaa tcctattaat 69960
gaggtattag cactcttata ttgacgaggt agtaatgcta tatacttaac ccagtgaggc 70020
acttgaatag tatgtttcat atattccaca gtcttgcctg taggatatgt aataatcatt 70080
tagtacctca caacgtatac tggtttatta agcctattca tgatatcaat catgtgcttg 70140
gttcctttgg atttaccatc ccagaaacat acagcagcat ctgcaaattc gcccatttct 70200
ttattcctgc ggaatccagc agatttgcca taggtatccc attcagcacg gaatacatgt 70260
atctgcatgt tttcagattt ccatagacgg aaagctaata tatctgcacc acgagccata 70320
ccacatacta attcaggatt agggtcaatc ttacctttct ccactaaatc cattaatact 70380
gtaatcatta cttgggtatt tacgaagtct ctgccaccag ctactattag tctcatttct 70440
gataaccttc tatgaagtta gtacgacgaa taagctgagt agatgcctta acagctgtct 70500
tcttgaactc atctttgtgt tggttttcca atatcttatc ccattccttc tggttccgtc 70560
gtttctgtag gatacgacta cacttcttat gactagcaac tcctcttcct ttattgcaaa 70620
tcgggcaaat accataatta gtgctgaaca acattttgct tactccttgg taatgtgatt 70680
ggcagtatag aagtcattaa tctctgattg ggtagtatca gcataaccgg atacaccggt 70740
ttctttatta acccatttat cgaagcactc actgtgtcca tgtgtactaa cttttaccca 70800
tgtactggtt ccaaagaatg tttttttctc tacttctgca ccaataccac gttcattatc 70860
tgcaaagcgt attaccttat atttagccat ttaatgcctc cattaatatt ttacgaataa 70920
cgttaacatc agactctgta ccaattaaac caacaggctt acctgtacta tctgttacaa 70980
aggatgcaat cctttccatc tctgctgtaa ttagcttgtc accactaata agacgattac 71040
ggaggtaagc ttcataagct acctctacat cttctaccgt ggttctttca ttacgagtga 71100
atggattact taatgggaat ggtaagtgga tacgtatatt ccggtcagct atcttagctg 71160
aatgaggaag tatacatact atttcattta gactcatcga ctaaccttaa tgcgtgtgac 71220
agtttcatta acataactgc atctgataac ttactagcat cactatggat gatagctcca 71280
tgattgccta gtgaatgata ttcttcactg gtttctttag cgtagttact aatagcttta 71340
gttacttcat atgcaccgta catacgacac atatactgta attgctcaat atgctcttgt 71400
tccattatct tctcctgttt aaatacatat ctaactattc aagaataaaa aatagaagcc 71460
ctcctaatgg aggactccca gttctactaa tgcaccatag attagcagtg caaacatggc 71520
tattgagata ggccagttca tttgaacaga tccgtaagta aatcgccgaa gcagtaccat 71580
atgtaccata ttacatatcc aacactggct cccttactta atacagtgat atatccgaat 71640
acagcgaatc ccctgacatc tcctttaata cgaaacattt tagtgatatt agctgcttct 71700
gaagtaagcc atacgcttaa gtagactaac gtgattaaga tagtgaaata cattttattc 71760
tcctaagatt gcagggtgat taaaggttac gatacgagct ttactttgct taagtcctga 71820
atcaatgaat tctttcagtt gttcattggt catgctgagt gctcgtttac ttgctacatt 71880
acgactacgc atacgagcaa catcttcctt ggttcccatg attaatgatt tacgcatgat 71940
ttagttgtcc acataaagtt aaaaggttta ctgttacgta caattcttac agccatttga 72000
tagccattga gttgtacaac accatagtgt cgtttctgta caccagtgtg atacctacga 72060
aagatacatt cttcaatggc atcatctatg ttagtgaata tcactggttc ctctttgcaa 72120
actcttctgc ttccttagcc cagagttgct tacgaacttc tttagctacg atggaagcaa 72180
acagacactt aacgaataca accggtaact tacagataag caccagctta gtgattaccc 72240
acttggctcc attgagaatg taaccacgta ggcgatacag cacatagcag agtattacaa 72300
acgcaatgaa gtccagcata gtcttatacc ttatgaagtt tacgtatttg tttacgagta 72360
ggtttagctg caaagaagtg gtcatagcag tgaccatttc catcttctac agatacttgc 72420
caacgagcat ttagatgcat tttactgccc caaggttgta acatttcaat caacttctgt 72480
gagttataac ggttacaaat tgtacgcata atggttactc cttcatctaa tataaatcta 72540
tatctatctg gttactgttt cagtaccctt cgtctaaaga tacccaaaaa gtagaaatag 72600
cttatcgtct ggttaaaaat aaaagactcc ccgaagggag tctagtagtt gattagaaag 72660
tcagtttacg agcatattta ttctcgtctg ctgggatgtc agcagcctct gctttcacac 72720
gacgaagttg aacttgcagg ttaatgatac cttcttcacc tggttccagg ttctgagcat 72780
attcaattaa ctgttccagc aagtcattct gtgcacaacg catagcacgg aagtcagcat 72840
tgctgctatt agtaggcagt ggctcctgag tatccagtgg aataccagta ggcaatgaga 72900
tgaatttctc atcatcagag ccttcattag ctacataacc aatgttcagc cagaactgag 72960
cttttggttt atcagcttta gcagtagaag cagcagcgtt agattgaccg aaagtgaatt 73020
tttgaataga cattttgtac tctccagata aatgattgtt tgatttatgg gtttgtccgt 73080
cggtgactaa acccaagaaa agacttagga tttcgataag aagtggattc attcttatcc 73140
tttagacgcg gagcgtcttg gtttttagaa gtagaagtaa agtagataaa tatctacttt 73200
tgactatcta tctaatcttt ataaaaggag actccgaaga gtctcccagg tgacttagtc 73260
tttagtcagg atgtcaatta ctttggaata ttctgcgtta tagatacgag 73310
Claims (4)
1.一株多重耐药性大肠杆菌噬菌体，其特征在于，该噬菌体为大肠杆菌噬菌体为RDP-EC-20155，保藏编号为CGMCC No.21992；透过电镜观察，该大肠杆菌噬菌体RDP-EC-20155有一个多面体立体对称的头部，包裹着核酸，直径68nm，有一个长160nm的尾，有尾鞘，颈部连着头部和尾部，为有尾病毒目肌肉尾病毒科。
2.如权利要求1所述的大肠杆菌噬菌体RDP-EC-20155在制备治疗大肠杆菌引起的疾病的药物中的用途。
3.一种用于防治大肠杆菌的杀菌组合物，其特征在于，包括有效量的如权利要求1所述的大肠杆菌噬菌体RDP-EC-20155。
4.一种清洁剂或消毒剂，其特征在于，包括如权利要求1所述的大肠杆菌噬菌体RDP-EC-20155。
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