KR102125235B1 - 닭 대장균 예방 및 치료를 위한 신규 박테리오파지 및 이를 이용한 항균조성물 - Google Patents

닭 대장균 예방 및 치료를 위한 신규 박테리오파지 및 이를 이용한 항균조성물 Download PDF

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KR102125235B1
KR102125235B1 KR1020140019121A KR20140019121A KR102125235B1 KR 102125235 B1 KR102125235 B1 KR 102125235B1 KR 1020140019121 A KR1020140019121 A KR 1020140019121A KR 20140019121 A KR20140019121 A KR 20140019121A KR 102125235 B1 KR102125235 B1 KR 102125235B1
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김태환
정태성
김기세
김채현
정청환
이종암
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주식회사 고려비엔피
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Abstract

본 발명은 닭 대장균 예방 및 치료를 위한 신규 박테리오파지 및 이를 이용한 조성물에 관한 것으로, 보다 상세하게는, 항생제를 대체하기 위해 양계에서 패혈증, 기낭염, 전안구염, 관절염, 수란관염 및 대장균성 육아종증 등의 여러 질병을 일으키는 대장균을 특이적으로 사멸시키는 박테리오파지 및 이를 유효성분으로 포함하는 닭 대장균증을 치료 또는 예방하는 조성물에 관한 것이다.

Description

닭 대장균 예방 및 치료를 위한 신규 박테리오파지 및 이를 이용한 항균조성물{New Bacteriophage for Prevention and Treating the Chicken Escherichia coli and Antibacterial Compositions Containing the Same}
본 발명은 닭 대장균 예방 및 치료를 위한 신규 박테리오파지 및 이를 이용한 조성물에 관한 것으로, 보다 상세하게는, 항생제를 대체하기 위해 양계에서 패혈증, 기낭염, 전안구염, 관절염, 수란관염 및 대장균성 육아종증 등의 여러 질병을 일으키는 대장균을 특이적으로 사멸시키는 박테리오파지 및 이를 유효성분으로 포함하는 닭 대장균증을 치료 또는 예방하는 조성물에 관한 것이다.
대장균은 조류 및 포유동물의 장내 정상 세균총으로 존재하면서, 대장균 그 자체 혹은 2차감염으로 내장이나 소화기관에 병변을 형성하거나, 다른 세균 및 바이러스와 혼합감염하여 다양한 질병을 발생시킨다. 닭에서 증상으로는 패혈증, 기낭염, 전안구염, 관절염, 수란관염 및 대장균성 육아종증 등의 여러 병형을 나타낸다.
닭 대장균은 전세계 어디나 분포되어 있는 세균으로서, 건조에 강하여 계분, 깔짚, 토양, 먼지, 부화기내의 솜털 등에 오염되어 입을 통한 감염, 호흡기감염이 많고, 복막염 및 수란관염이 발생하면 계란으로 직접 전염되는 난계대전염이 되며 난각에 부착되어 종란 속으로 침투한다. 특히, 사육환경이 나쁘고 환기가 불량하면 자체 내에서 흔히 발생하며 계사 내에 암모니아가스로 호흡기계의 기낭염 및 심낭염이 나타난다.
대장균의 주요 증상은 6∼10주령의 닭에서 흔히 발생하며, 종란에 대장균이 이행되었을 경우에는 부화율이 떨어지고, 초생추에서도 발생하여 제대염을 일으키는 경우도 있어 증상은 다양하다.
대장균성 패혈증은 주로 10주령 이하의 닭에서 발생하여 폐사율은 5∼20%에서 50%까지 이르기도 하고, 병아리는 백색설사 또는 황녹색의 설사와 배가 불룩하게 부어오른다.
대장균과 관련된 질환을 살펴보면, 먼저, 종란에 대장균이 이행되어 부화율이 떨어지고 중지란이 발생하며 난황 흡수가 좋지 않고 제대염을 일으키기도 하고, 배가 불룩하게 부어오르면서 부화 후 2 ~ 3일이면 폐사하게 된다.
또한, 관절활막염은 만성으로 경과하면 관절이 붓고 다리를 절름거리게 된다.
또한, 수란관염 및 복박염: 복강내의 기낭에 대장균이 침입하면 기낭염에서 복막염 및 수란관염으로 진행되, 수란관이 붓고 염증이 생겨 때로는 폐사한다.
또한, 장관점막에 출혈 및 궤양이 생기며 출혈성장염 발생할 수 있다.
또한, 대장균성 육아종증은 소장, 맹장 및 간에 사마귀 모양의 육아종 병변이 특징 발견되기도 한다.
한편, 박테리오파지는 1917년 d'Herelle에 의하여 처음 발견되었고, 숙주세포인 세균에 감염되어 숙주세포의 생체기능을 이용 증시하는 바이러스로 자연계에 널리 분포하고 있다. 박테리오파지는 파지(phage)라고도 하며, 숙주간 높은 특이성을 갖는 특정 미생물만 감염할 수 있는 바이러스이다. 그러므로, 동물바이러스나 식물 바이러스와는 다르고, 동물세포나 식물세포는 감염할 수 없다. 동물 바이러스와 마찬가지로 숙주가 없으면 증식할 수 없음. 반면, 숙주만 있으면 쉽게 증식된다.
박테리오파지는 같은 속 (genus) 내에서만 감염 할 수 있다. 그러므로 리스테리아 파지는 대장균이나 젖산균에 감염할 수 없으며, 리스테리아 속 내에서 종이나 혈청형에 따라 감염 가능한 숙주균이 결정된다. 이를 숙주균주 스펙트럼 (spectrum)이라고 함. 숙주균주 스펙트럼이 넓은 파지일수록 더 많은 수의 균주를 사멸 할 수 있다.
박테리오파지의 증식경로를 설명하면 다음과 같다. 박테리오파지는 미생물의 세포 표면에 위치한 특정 수용체 (receptor)를 인식하고 흡착한다.
유전자를 세포 안으로 주입시키고 미생물 내부의 중합효소를 이용해 복제, 전사 및 번역반응을 진행한다.
1개의 단일 박테리오파지는 40-100여 개로 증식되어 미생물 밖으로 나오게 되며, 이때 미생물은 사멸하게 됨. 이런 과정을 거쳐 나온 박테리오파지를 ‘용균성 파지 (Lytic phage)’라고 명명한다.
어떤 박테리오파지들은 유전자가 세포 안으로 주입 된 후 미생물 게놈 (chromosome)에 삽입되고, UV나 항생물질의 자극을 받기 전 까지 숙주 미생물과 함께 존재한다. 이런 박테리오파지를 ‘용원성 파지 (Lysogenic phage)’ 라고 명명한다.
UV나 항생제의 자극을 받게 되면 용원성 박테리오파지의 유전자는 숙주균의 게놈에서 분리되고, 미생물의 중합효소를 이용하여 복제, 전사 및 번역 과정을 거쳐 숙주균의 세포막(벽)을 파괴하며 밖으로 나온다.
FDA를 포함한 많은 과학자들은 용원성 파지의 유전자가 숙주균주의 게놈에 직접 연관성을 갖게 되므로, 이로 인한 파지 내성을 유도 할 수 있다는 점을 고려해, 용균성 파지만을 식품 첨가제로 허가한다.
본 발명자는 대장균을 선택적으로 사멸시키는 파지를 발견하여, 이를 닭을 비롯한 가축의 항균 조성물로서 이용하고자 본 발명을 하게 되었다.
상기와 같은 종래기술의 문제점을 해결하기 위하여, 본 발명은 닭의 대장균에 대하여 특이적 사멸능을 갖는 신규 박테리오파지를 제공하는 것을 목적으로 한다.
본 발명의 다른 목적은 상기 박테리오파지를 유효성분으로 포함하는 닭의 대장균 관련 질병의 예방 또는 치료용 조성물을 제공하는 것이다. 구체적으로 항생제로 사용되는 예방 또는 치료용 조성물을 제공하는 것이다.
상기와 같은 목적을 달성하기 위해 본 발명은 대장균에 특이적 사멸능을 갖는 균주기탁번호(KCTC18269P)의 박테리오파지 ECBP1 또는 균주기탁번호(KCTC18270P)의 박테리오파지 ECBP2를 제공한다.
또한, 본 발명은 상기 박테리오파지를 유효성분으로 포함하는 대장균에 의해 유발되는 닭 대장균증의 예방 또는 치료용 항균조성물을 제공한다.
또한, 본 발명에서 상기 균주기탁번호 (KCTC18269P)의 박테리오파지 ECBP1는 서열번호 1을 가지며, 균주기탁번호(KCTC18270P)의 박테리오파지 ECBP2는 서열번호 2를 가지는 것을 특징으로 한다.
또한, 본 발명은 상기 박테리오파지를 유효성분으로 포함하는 닭 대장균증의 예방 또는 치료용 항생제를 제공한다.
또한, 상기 박테리오파지의 유효농도는 104 ~ 1012 PFU/㎖ 인 것이 바람직하다.
또한, 본 발명은 상기 박테리오파지를 유효성분으로 포함하는 닭 대장균증의 예방 또는 치료용 사료첨가제 또는 음수 또는 분무를 통해 공급하는 액제 또는 분말제제 또는 동결건조제제를 제공한다.
본 발명의 신규 박테리오파지는 닭의 대장균에 특이적 사멸능을 가지며, 내산성 및 내열성이 뛰어난 특성을 갖는다. 따라서, 본 발명의 박테리오파지는 닭 대장균증의 예방 및 치료에 효과적으로 사용될 수 있으며, 식염수, 증류 등과 희석하여 사용할 수 있고, 통상의 다른 제제와 혼합하여 투여 할 수 있다. 양계 대장균을 효과적으로 사멸 또는 억제함으로서 닭에서 효과적으로 관련된 질병을 억제할 수 있다.
도 1은 (도1)ECBP1(왼쪽), ECBP2(오른쪽)의 전자현미경 사진이다. 형태적학으로 정이십면체의 머리와 수축성이 없는 꼬리를 갖는 Podoviridae에 속한다.
도 2는 ECBP1, ECBP2 의 단백질 전기영동 결과를 나타낸 것이다.
도 3 은 ECBP1, ECBP2 의 RFLP 사진을 나타낸 것이다. ECBP1, ECBP2의 genomic DNA를 EcoR1 enzyme을 이용하여 digestion한 후 그 패턴을 확인한 결과, 서로 다른 패턴을 보였다.
도 4 는 ECBP1, ECBP2 의 내산성 실험 결과를 나타낸 것이다.
도 5는 ECBP1, ECBP2 의 내열성 실험 결과를 나타낸 것이다.
도 6은 ECBP1 유전자 지도 모식도를 나타낸 것이다.
도 7은 ECBP2 유전자 지도 모식도를 나타낸 것이다.
도 8은 공격접종을 위한 LD50 실험에서 닭의 간과 심장의 종대 및 염증을 나타낸 것이다.
도 9는 공격접종을 위한 LD50 실험에서 닭의 소장 닭의 소장 충출혈(A) 부검소견 및 간에서 대장균 재분리(B)를 나타낸 것이다.
본 발명은 대장균에 특이적으로 감염되어 사멸시키는 능력을 가진 신규 대장균 박테리오파지 ECBP1, ECBP2에 관한 것이다.
이하, 실시예를 통하여 본 발명을 상세히 설명하나, 이들이 발명의 내용을 제한하는 것은 아니다.
대장균 박테리오파지는 전자현미경 관찰결과 형태학적으로 수축성이 없는 꼬리를 가지며 모양은 정이십면체, 외피는 없는 podoviridae에 속하는 박테리오파지이다.
대장균을 선택적으로 사멸시키는 파지로 ECBP1는 게놈크기가 69.8kb로 T7-like RAN polymerase와 62개의 hypothetical protein, 2개의 tRNA가 encoding되어 있는 유전자구조로 유전자 분석 결과, 형태학적 결과와 동일하게 podoviridae로 확인되었다.
ECBP2는 게놈크기가 77.3kb로 portal protein, major head protein, tail fiber protein 및 DNA manipulation에 관여하는 단백질, 100개의 hypothetical protein, 1개의 tRNA가 encoding되어 있는 유전자구조로 유전자 분석 결과, 형태학적 결과와는 달리 unclassified phages로 확인되었다.
대장균에만 특이적으로 감염되어 사멸시키며 다른 병원성 균주인 황색포도상구균, 살모넬라균과 슈도모나스균을 이용하여 plaque assay를 실시하여 특성을 확인해본 결과, 다른 병원성 균주에는 용균성이 없는 것으로 나타났다.
대장균에 특이적인 사멸능력을 갖는 박테리오파지 ECBP1는 균주기탁번호(KCTC18269P), 박테리오파지 ECBP2는 균주기탁번호(KCTC18270P)로 2014년 1월 17일에 한국생명공학연구원 미생물자원센터에 기탁하였다.
본 박테리오파지 ECBP1, ECBP2는 충남지역의 양계장 인근 생활하수에서 분리한 박테리오파지로 E. coli 표준균주와 함께 박테리오파지 표준 분리법(double-layer plaque assay)에 따라 실험하여 순수분리 하였다. 분리된 파지를 이용하여 양계장에서 분리한 대장균을 이용하여 항균능력을 확인한 결과 분리된 파지는 양계 병원성 대장균에 특이적으로 감염되어 사멸능력을 갖고 있다. 전자현미경에 의한 형태학적으로는 podoviridae에 속하는 것으로 확인되었으며 유전자 분리 RNase와 DNase 처리 후 전기영동 한 결과, DNase 처리한 경우에는 핵산이 분해되어 밴드를 확인할 수 없었으며, RNase를 처리했을 때는 밴드를 관찰 되어 파지 모두 DNA를 가지고 있음을 확인하였다.
ECBP1, ECBP2의 pH 7.5의 파지 생존을 100%로 하여 pH 안정성을 확인한 결과, 생존율이 약 80% 이상으로 산성과 알카리성에도 비교적 안정한 파지로 확인되었다.
ECBP1, ECBP2의 60℃에서 1시간 처리 전의 파지 생존을 100%로 하고 60℃에서 1시간 처리 후 안정성을 확인한 결과, ECBP1 생존율이 약 75%, ECBP2 약 85% 이상으로 가온조건에서도 안정성을 확인하였다. 내산성, 내열성을 갖는 특징은 이를 이용한 다양한 대장균 질병 제어 제품을 생산, 개발하는데 있어 많은 장점을 가질 수 있다.
항생제 내성이 확인되었거나 민감성이 확인된 닭 대장균을 확보하여 박테리오파지와 반응하여 용균가능 여부를 확인한 결과 ECBP1, ECBP2 모두 양계 대장균에 대하여 용균반을 형성하며 양계 대장균의 생육을 억제하는 것이 확인 되었다.
일반적으로 ECBP1, ECBP2 박테리오파지 배양은 숙주 대장균이 배양되고 있는 배양용 배지에 0.01~0.1MOI(Multiplicity of Infection) 농도로 접종되며 일반적으로 30℃에서 배양하고 획득되는 박테리오파지의 수율은 109PFU/㎖ 이상으로 높은 수율로 배양되어 산업성과 경제성이 높다.
< 실시예 1 : 박테리오파지의 대장균 용균 실험>
본 발명의 박테리오파지는 대장균을 숙주 미생물로 하는 Bacteriophage ECPB1, Bacteriophage ECPB2이다. 원주는 2011년 생활하수, 양계농장 하수에서 분리된 Bacteriophage ECPB1, Bacteriophage ECPB2주로서, E. coli을 특이적으로 용균하는 박테리오파지이다.
이의 확인을 위해, 다음의 실험을 시행하였다. 상기 박테리오파지를 희석 완충액(100mM NaCl, 50mM Tris-HCl(pH7.5), 10mM MgSO4, 0.01% gelatin)으로 용해시키고 E.coli이 포함된 0.7% 연한천에 혼합한다. 이를 한천배지에 도포하고 고체화시킨 후 30℃에서 24시간 배양하면 용균반이 나타난다.
E.coli 용균반을 형성되고, Staphylococcus aureus, Staphylococcus sp21, Staphylococcus sp22, S.typhimurium KOLASB3-1, S.typhimurium CCARM0277, Salmonella sp.21, Salmonella sp.22, P.aeruginosa 에 대해 용균반을 형성하지 않는다.
< 실시예 2 : 대량배양 조건 확립>
5L fermenter(KBT-250)를 이용한 배양 조건을 확립하였다. 박테리오파지 배양을 위한 최적의 배지를 선택하기 위해 LB(Luria Bertani broth), TSB(Tryptic Soy broth)를 사용하여 배양 비교실험 결과 생산성에는 큰 차이가 없어 일반적으로 대장균을 배양하는 LB를 사용하여 대량생산 조건을 확립하였다.
박테리오파지 생산을 위한 배양조건은 배양온도 30℃, aeration: 2.5~3 교반 수 150rpm, pH는 6.8~7.4을 기본 조건으로 하여 생산 수율을 확인하였다(표 1).
< 박테리오파지 host 균주 배양 및 MOI 에 따른 수율 >
구 분 Titration
접종시간 별
(host 균주 배양)
(파지 O/N 배양) 		8시간 배양(OD1.69) 2.4x10 9 PFU/㎖
6시간 배양(OD1.55) 1.3x10 10 PFU/㎖
4시간 배양(OD1.50) 4.1x10 9 PFU/㎖
MOI
(host 8시간 배양)
(파지 O/N 배양) 		0.01 9.3x10 9 PFU/㎖
0.1 3.2x10 10 PFU/㎖
1 3.8x10 10 PFU/㎖
숙주 대장균 배양 4~6시간 배양하여 OD(600nm) 1~1.5정도 배양하여 박테리오파지 접종 했을 때가 박테리오파지 생산 수율이 높게 나타났다. 박테리오파지 접종량은 0.1~0.01MOI(Multiplicity of Infection)가 되도록 접종하고 overnight 배양한 경우가 생산성이 높았고 생산된 박테리오파지 titration 결과 109PFU/㎖ 이상 이었다. 5L Fermenter의 배양조건을 기초하여 1ton/batch 대량생산 조건을 확립 하였다.
<실시예 3 : 대장균에 감염하는 박테리오파지의 분리, 순수분리>
박테리오파지 배양은 일반적으로 대장균을 배양하는 LB(Luria bertani ; tryptone 10g, yeast extract 5g, NaCl 10g 전체부피 1L)를 사용하여 생산 조건을 확립하였다. 양계장 주변 채취한 하수 20ml를 50ml tube에 옮겨 대장균(OD600=1.5) 5ml, LB broth 10ml 넣고 30~27℃에서 16~18시간 배양 후 4,000rpm에서 20분간 원심분리 후 0.2㎛ filter를 이용하여 여과하였다. 여과한 액은 LB palte에 대장균(OD600=1.5) 배양액 100㎕를 0.7% 연한천 LB배지에 넣어 잘 섞어 부어준 후 30분 이상 굳히고 그 위에 채취하여 배양한 하수 시료 5㎕씩 떨어 뜨려 30~27℃에서 18~24시간 배양하고 용균을 확인하였다.
용균반이 생성되었으면 순수분리를 위하여 용균반을 채취하여 LB broth 4ml에 대장균 10㎕를 넣고 배양한 후 4,000rpm에서 20분간 원심분리하여 상층액을 0.2㎛ filter를 이용하여 여과한 후 위와 같은 방법으로 연한천 배지에서 다시 용균반을 생성하는 실험을 3회정도 반복하여 순수 분리하였다. 순수분리된 박테리오파지 용균반에 SM buffer(Nacl 100mM, MgSO4 8mM, Tri-HCl 50mM)를 넣고 상온에서 4시간 이상 정치하여 박테리오파지를 회수하였다. 회수된 박테리오파지는 4,000rpm에서 20분간 원심분리 후 0.2㎛ filter를 이용하여 여과한 상층액을 냉장보관 또는 글리세롤이 50%가 되도록 하여 냉동보관하였다. 이러한 방법으로 생산된 박테리오파지의 농도는 109PFU/㎖이상이었다.
<실시예 4 : 형태관찰>
배양하여 순수분리한 박테리오파지를 DNase I(150unit), RNase(1ug/㎖), 0.2% chloroform이 되도록 첨가하여 실온에서 30분간 반응한 후 0.5M NaCl에 녹여 4℃에서 한 시간 이상 정치하였다. 6000~8000g, 4℃에서 10분간 원심 분리한 뒤 상층액에 PEG6000을 10% 농도가 되도록 넣은 다음 완전히 녹인 후 4℃에서 한 시간 이상 정치하였다. PEG 처리한 박테리오파지를 10,000g, 4℃에서 15분간 원심분리한 후 상층액을 버리고 SM buffer에 원심분리 pellet을 재부유하여 실온에서 15분정도 정치하였다. 이와 같이 분리된 박테리오파지를 CsCl gradient를 이용하여 25,000rpm, 4℃, 2시간 원심분리하여 고농도로 순수 분리된 박테리오파지(ECBP1, ECBP2)를 정제하였다.
정제된 박테리오파지를 400 mesh copper grid에 8㎕ 떨어뜨려 2분간 반응시간 후 건조시키고 1% uranyl acetate호 10초간 반응하여 염색시키고 건조하였다. 건조가 완료된 샘플을 120kV TEM(전자투과현미경)으로 관찰하였다. 전자투과현미경 사진은 도 1이다. 분리정제된 박테리오파지는 전자투과현미경 관찰결과 형태학적으로 비수축성 꼬리와 정이십면체의 podoviridae에 속하는 것으로 확인되었다(도 1 참조)
< 실시예 5 : SDS - PAGE 를 이용한 단백질 특성 분석>
PEG 침전과 CsCl Gradient를 통해 순수 분리된 박테리오파지를 이용하여 SDS 전기영동을 실시한 결과를 도 2에 나타내었다. 1은 ECBP1 host 균주, 2는 ECBP1, 3은 ECBP2 host 균주, 4는 ECBP2를 나타낸 것이다. ECBP1은 5개의 major band(32kDa, 35kDa, 38Kda, 40kDa, 70kDa)와 9개의 minor band 패턴을 보인다. ECBP2는 2개의 major band(37kDa, 55kDa)와 12개의 minor band 패턴을 보인다. ECBP1과 ECBP2는 서로 다른 단백질 패턴을 보였다.
< 실시예 6 : 유전자 분석>
핵산 추출 방법은 0.45ml phage suspention에 4.5㎕ DNase I(1mg/ml), 2.5㎕ RNase(10mg/ml)을 혼합한 후, 37℃에서 30min 동안 Incubation을 한다. 여기에 23㎕ 의 10% SDS과 2.25㎕의 protease K(20mg/ml)를 추가하여, 혼합한 후, 37℃에서 30min 동안 incubation한다. 동량의 PCI solution 첨가하고, 2~3min간 4℃에서, 1500g/5min 조건으로 vortexing을 실시한다. 수층의 것을 새 Tube로 옮기고, 동량의 Chloroform 첨가하여, 6000g/5min 조건으로 vortexing을 실시한 후, 수층의 것을 새 tube로 옮긴다. pH5.2 약 45㎕의 1/10 3M Potassium acetate를 추가하고, Add 2.5 volume의 100% EtOH를 추가한 후, RT에서 20min간 14000rpm/20min (4℃)조건으로 실시하였다. 70% EtOH로 2~3회 세척하고, 약간 건조 후 TE buffer(10mM Tris, 1mM EDTA, pH8.0) 로 pellet을 녹인다.
박테리오파지의 Genotype을 확인하기 위해, 박테리오파지의 핵산을 분리하여 Lamda maker를 size marker로 하여 전기영동 한 결과, 분자량 size marker 23kb 위쪽에 위치하는 밴드를 관찰하였다(도 3 참조).
RNase와 DNase 처리 후 전기영동 한 결과, DNase 처리한 경우에는 핵산이 분해되어 밴드를 확인할 수 없었으며, RNase를 처리했을 때는 밴드를 관찰 되어 파지 모두 DNA를 가지고 있음을 확인하였다(도 3 참조).
< 실시예 7 : ECBP1 유전자 지도 모식도>
ECBP1을 shotgun 시퀀싱을 통해 전체 유전자를 분석하였다. 총 69,855bp 길이를 가지는 double-stranded DNA phage로 42.74%의 GC contents와 총 82개의 ORFs중 구조 및 전사에 관련 있는 단백질 중 특히 RNA 전사에 가장 중요한 영향을 끼친다고 알려진 T7-like RAN polymerase를 확인하였고 이외에 62개는 hypothetical protein으로 annotation되었고 2개의 tRNA가 encoding 되어 있다. 유전자 분석결과 형태학적 결과와 동일하게 podoviridae 로 확인되었다. Sanger’s institute의 DNA plotter를 이용한 모식도이다(도 6 참조).
< 실시예 8 : ECBP2 유전자 지도 모식도>
ECBP2를 shotgun 시퀀싱을 통해 전체 유전자를 분석하였다. 총 77,315bp 길이를 가지는 double-stranded DNA phage로 42.42%의 GC contents와 총 120개의 ORFs중 형태발생에 관여하는 portal protein, major head protein과 tail fiber protein과 DNA manipulation 관련인자를 확인하였다. 이외에 100개의 hypothetical protein으로 annotation되었고 1개의 tRNA가 encoding 되어 있다. 유전자 분석결과 형태학적 결과와는 달리 unclassified phages로 확인되었다. Sanger’s institute의 DNA plotter를 이용한 모식도이다(도 7 참조).
< 실시예 9 : 내산성 - pH 변화에 따른 박테리오파지 안정성 확인 >
ECBP1, ECBP2의 pH 7.5의 파지 생존을 100%로 하여 pH 안정성을 확인한 결과, 생존율이 약 80% 이상으로 산성과 알카리성에도 비교적 안정한 파지로 나타났다(도 4 참조).
< 실시예 10 : 내열성 확인 - 온도 안정성 조사>
ECBP1, ECBP2의 60℃에서 1시간 처리 전의 파지 생존을 100%로 하고 60℃에서 1시간 처리 후 안정성을 확인한 결과, ECBP1 생존율이 약 75%, ECBP2 약 85% 이상으로 안정한 파지로 나타났다(도 5 참조).
< 실시예 11 : 다른균주에 대한 감염력 조사>
다른 병원성 균주를 이용한 특성을 분석한 결과는 다음의 표 2와 같다.
Figure 112014016300346-pat00001
< 실시예 12 : 분리 대장균의 병원성 실험- LD 50 실험 >
양계 농장에서 분리한 대장균이 닭 병원성 대장균인지 확인하기 위하여 7일령 SPF 병아리를 이용하여 접종 실험을 하였다. 분리된 대장균을 병아리에 108CFU/㎖량으로 복강 접종하였다.
대장균을 접종한 병아리에서 백색설사, 배가 볼록하게 부어오르는 등 육안적인 병증을 확인하였고, 부검 소견에서는 대장균증에 의한 복막염, 심낭염, 간 섬유소 유착, 간 종대, 소장 충출혈 및 가스 생성 등이 확인되어 대장균증 감염이 의심되었다.
폐사계 간에서 대장균 재분리가 되어 닭대장균증에 의한 폐사가 확인되었고 농장에서 분리한 대장균이 병원성 닭대장균임이 확인되었다.
< 실시예 13 : 공격접종을 위한 LD 50 실험 >
공격접종을 통한 닭 대장균증 예방에 관한 항생제 대체효과를 살펴보고자 실험을 진행하였다. 실험동물은 7일령 SPF 병아리를 그룹당 10마리 사용하고, 대장균 접종은 대조군, 107, 108, 109, 1010CFU/㎖로 접종하여 관찰하였다.
접종 3일 후 설사 및 폐사 발생가 발생하였고 부검소견상 닭대장균증의 임상 증상이 발견되었다. 각 그룹별 폐사계를 확인한 결과 LD50 108CFU/㎖으로 결정되었다(도 8 및 도 9 참조).
< 실시예 14 : 유효성 평가 실험>
박테리오파지 최상의 투여방법 확인을 위해 동물 실험은 3차까지 진행 하였으며 1차 동물실험에서는 1종(파지명: ECBP1)의 박테리오파지 접종량은 1㎖이었으며, 파지 음수투여 후 바로 109LD50으로 1㎖ 공격접종을 하였다. 실험 결과 대조군, 실험군에서 폐사율의 차이가 없었다. 이는 파지가 위산에 의해 저해되고 1종의 파지를 접종하여 효율성이 낮고 공격접종량이 많아 부작용이 발생한 것으로 판단된다.
2차 동물실험에서는 2종(파지명: 14, 16)의 박테리오파지를 각각 0.5㎖씩 접종하였고, 파지 음수투여 후 109LD50으로 1㎖을 공격접종하였다. 실험 결과 대조군, 실험군에서 폐사율의 차이가 없었다. 이는 파지가 위산에 의해 저해되고 공격 접종량이 많이 부작용이 발생한 것으로 판단된다.
3차 동물실험에서는 2종(파지명:ECBP1, 2)의 박테리오파지를 각각 0.25㎖씩 접종하고 파지 음수투여 후 109LD50으로 0.2㎖을 공격접종하였다. 실험 결과 대조군에서는 100% 폐사, 실험군에서 50~62.5% 폐사가 발생하였다. 이는 파지의 접종 투여량을 1, 2차 실험의 1/4~1/2로 줄이고, 공격 접종량도 1/5로 줄여 접종에 의한 부작용을 줄여 효과적으로 박테리오파지가 대장균을 억제한 것으로 보여진다. 또한, 박테리오파지가 위산에 의해 저해되는 것을 막기 위해 위산 중화제와 박테리오파지 보호제를 이용하여 투여한 것이 시너지 효과를 나타낸 것으로 보인다(표 3).
Activity를 보존하기 위한 최상의 투여방법 확립 및 파지 유효성 동물실험
구분 1일후 2일후 5일후 6일후 7,8, 9일후 결과
10 5 PFU /㎖ 5/8 5/8 5/8 5/8 5/8 62.5% 폐사
10 6 PFU /㎖ 4/8 4/8 5/8 5/8 5/8 62.5% 폐사
10 7 PFU /㎖ 4/8 4/8 5/8 5/8 5/8 62.5% 폐사
10 8 PFU /㎖ 2/8 2/8 3/8 4/8 4/8 50% 폐사
control 3/8 5/8 8/8 - - 100% 폐사
본 발명에서 파지는 단순히 치료제만으로 전적으로 사용될 수 없는 부분이 많고, 그래서 박테리오파지를 사료 혹은 음수에 첨가하여 사육환경에서 병원성을 지닌 특정 세균의 절대적인 총균수를 줄임으로서 생산성을 향상 시키고자 하는 것이다. 또한, 이러한 환경에서 박테리오파지에 저항성 있는 세균이 등장하더라도 병원성 약해져 기회 감염의 수준을 낮추는데 의의가 있다.
이러한 관점에서 검토하여 보면 음수로 적용하는 데는 대조군과 폐사율에서 38 ~ 50% 정도의 차이는 상당히 큰 차이로 판단되며 음수 외에 사료첨가제 형태 또는 혹은 분무형태의 환경개선제로 병행하면 사양환경을 개선함으로써 얻어지는 2차 효과를 바라볼 수 있다. 또한, 박테리오파지는 파지 단독으로 사용으로 사용할 수 있고 질병이 있을 경우엔 항생제등과 함께 사용함으로서도 시너지 효과를 볼 수 있다.
상기에 제시된 실시예는 예시적인 것으로 이 분야에서 통상의 지식을 가지는 자는 본 발명의 기술적 사상을 벗어나지 않는 범위에서 제시된 실시예에 대한 다양한 변형 및 수정 고안을 만들 수 있을 것이다. 이러한 변형 및 수정 고안에 의하여 본 발명의 범위는 제한되지 않는다.
기탁기관명 : 한국생명공학연구원
수탁번호 : KCTC18269P
수탁일자 : 2014117
기탁기관명 : 한국생명공학연구원
수탁번호 : KCTC18270P
수탁일자 : 2014117
<110> KBNP, INC <120> New Bacteriophage for Prevention and Treating the Chicken Escherichia coli and Antibacterial Compositions Containing the Same <130> P-2014-0001 <160> 2 <170> KopatentIn 2.0 <210> 1 <211> 69855 <212> DNA <213> Artificial Sequence <220> <223> Escherichia phage KBNP21(ECBP1), complete genome <400> 1 ttagcaaaga tgctaccgta aggaatagta gtggtaatcc taaccatccg gactattact 60 ctagtatagc acgcatcatc accagaaagc cccatcttac aggataactt acacaattaa 120 gctcttaagg atattaaatg aaacttacta agataggtta tgctgatgga cctatttcca 180 ctacccatac aaccttacaa gaccttgtac ctggtagact cactcctatt atcctagtac 240 cagatgatca gactctacac gaagttaaag gattagcttt actactaaat aaggtgacta 300 ttgtagaagt tcttgaacca ggaaaggaat taccgtggtc ctcctacatg tactccccct 360 ggcatgtgaa tacggataag tacactctta cctttggtat taccagtgaa ctgcaaactc 420 ctcacttggt atatgcttcc tcctatttaa ctgcttataa gtaggatagc tatcatattt 480 ttgcatatga aaagtagcat agttatgtat ggctacagtg gtgtggctgg ggctacaccc 540 ttacactcaa ccatcccccc cggtatgttg acggtgtagc cactacccta ccccacctta 600 cacgttacac atgctccgca tgttatggac atgtcgtcct atcatcaact attggagcta 660 tcatcatgac tactcaagct actgtacgta tgaccgcagg aactttactt ggtactgtta 720 actcagctgc tactactgtt gcagatacat tcggtacagc aactaaagca gtaggtatgc 780 ttaattcata tgttagtacg atggcagaga aacaagccat tcgtactaaa ttagaaatgc 840 atacctttgt taataaactg gcagaggaaa cagctatgac tgagactctg cgtaagaaaa 900 gtatcgagga attctgcaag gattcagaga acgctcgaat ctataacgca gaatattcca 960 aagtaattga catcctgact aaagactaag tcacatagga gactcttcgg agtctccttt 1020 tataaacatt agatagatag tcaaaagtag ataactatct actttggaga tagataatgg 1080 ctaagtacat ttgcataaaa tcattaacta aagatgttcc tgtaggaaca gaactggtag 1140 gaaaatacca ctcaccagga agattcattc ttactgaaga tagtaaatta aagtgtgtat 1200 caggaacagg accacgttgg tacaaagatg accaactacc tcttgcaggt gctttatggg 1260 tatggcaatg ccaagaccct attaaagctt tagaacgcta tgagaaagct gttcgtgccc 1320 atgaaatgct tggtgctcaa gacccggaag attggcctgg aattgagaaa gagtatgaat 1380 ctgctaaggc agaattgctt aatcatttgt attaaaaacc aagaccgcag gcgttgtctg 1440 caagacaacg acgctccgcg tcttatggat gatgtaaatg catccactaa tcattgaact 1500 tttatccctt tgtttgggtg ttctcaccga cgagttcacc cgtaaatcac ataaatctgg 1560 agagtacaaa atgtctattc aaaaattcac tttcggtcaa tctaacactg ctgcttctac 1620 tgctaaaact gataaaccaa aagctcagtt ctggctgaac attggttatg tagctaacga 1680 aggctctgat gatgagaaat tcatctctct gcctactggt attccactgg atactcagga 1740 gccactgcct actaatagca gcaatgctga cttccgtgct atgcgttgtg cacagaacga 1800 cttgctggaa cagttaattg agtatgctca gaacctggaa ccaggtgaag aaggtatcat 1860 aaacctgcaa gttcaacttc gtcgtgtgaa agcagaggct gctgacatcc cagcagacga 1920 gaataaatat gctcgtaaac tgactttcta atcaaccaat agactccctt cggggagtct 1980 tttattttta actagacgat aagctatttc tactttttga gtatctttag acgaagggta 2040 ctgaaacagt aaccagatag atatagattt atattagaag aggaattaac catgttagac 2100 ttcatcgctt tctgtgtaat cttctacttt ctgggttgga agttacgtca taaatggttg 2160 cttattgcta aactgccatt cgttactatc gagtgtctca ttgctagtat taaacacaag 2220 aaagcaatga ctcagtacta caagcaacaa gcagaagagt ttgcaaagag gaaccagtga 2280 tattcactaa catagatgat gccattgaag aatgtatctt tcgtaggtat cacactggtg 2340 tacagaaacg acactatggt gttgtacaac tcaatggcta tcaaatggtt gtaagaattg 2400 tacgtaagaa taaacctttt aactttatgt ggagtactaa atcatgcgta aatcattaat 2460 catgggaacc agagaagacg tgcaaaagat gaaagaacgt ctggttgcta agaaacagac 2520 gtctgaacca gtacgtaaga ttgttacctt caaccattca tgtatcaagt aactaggagt 2580 cctccattag gagggcttct attttttatt cctgaatagt tagatatgta tttaaatagg 2640 agaaataacc ttgaatgaaa ttgtttgtat acttcctcaa tcagctaaga tagctgacag 2700 gaatatccgt atccacttac cctttccatt aagtaatcca ttcactcgta atgaaagaac 2760 cacagtagaa gatgtagagg tagcttatga agcttacctc cgtaatcgtc ttattagtgg 2820 ggacaaacta attacagcag agatggaaag gattgcatcc tttgtaacag atagtacagg 2880 caagcctgtt ggcttaattg gtacagagtc tgatgttaac gttattcgta aaatattaat 2940 ggaggcatta aatggctaaa tataaggtaa tacgctttgc agataatgaa cgtggtattg 3000 gtgcagaagt agagaaaaaa acattctttg gaaccagtac atgggtaaaa gttagtacac 3060 atggacacag tgagtgcttc gataaatggg ttaataaaga aaccggtgta tccggttatg 3120 ctgatactac ccaatcagag attaatgact tctatactgc caatcacatt actaaggaat 3180 aaacaaaatg ttgttcagca ctaattatgg tatttgccca atttgcaata aaggaagagg 3240 agttgctaat cataagaagt gtagtcgtat attacagaaa caacggaatc agaaagaatg 3300 ggataaggtg ctgaataatc agcataaaga agagaatcaa cagatggctg ttaaagcatc 3360 tactcaacgt attcgccgta ttaactatat acaggggtat cagaaatgat tattgcatat 3420 cctacaggca agactgtgga atatatgaag catactattc aagtgcctca ctgggttaag 3480 tatatagcat tactacctcg tcaatataag agtgctaata cctcactaat aggattctct 3540 aaaaaaccta agctaacaga aaacaatatc tgggtatcct ctgggagaca agaagagatt 3600 ggtttttgtg atttgagtat tgtcaataat aacgtttacc taacgctgga gaaagtagca 3660 tgaatattga aaattatgaa atcactttag taacagcaga cagtaaaact gtgattaaca 3720 aacaattaaa taatgaccct gagatgctta actgggttgc agagcaattt gctgatgtta 3780 actcagcaca ggtaacccta cggaatatgt caggaaaagt agtagcattt acaggcaagg 3840 agccagtatg aaaactattc tagtaatcca tgattctacg tttactgatg tagataagat 3900 gatgcgtaat attgattatg tatctcagac aagccaagca ttcaatgaag agtttactct 3960 gtactgcaat gctgaatctc ctctggttcc tatccttaag gaatcaggtc taccattctc 4020 tacagagaac ttcccggaag aaccagacta tgtaatctca tttatctatg acttacatga 4080 tggttctgaa actagtgaat tagctatgaa ccagtggcgt agtaaacgtc ctgtgtttgc 4140 atttcaggta cttaaaccat gaagattatg tcattaggtg atgacaccaa tgctttactt 4200 ggtgtatctg cacgtccaat tatcattgtt aataaacatc acggtaaatc cggtgagtat 4260 attgggcgtg gctcaccatt aggcaatcca ttcgtcattg gtaaacatgg aaccagagag 4320 caagtaattg ctaagtataa agtctggtta caagaacaga ttgataaagg taatccagtt 4380 gtactggatg aacttaatcg tctgggtaat aaagccattg atgagaaagg attagcctta 4440 cagtgcttct gttatccaaa accatgtcat ggtgaggtta ttaaagagaa gctagtaaag 4500 gctatgtata actactttgt agaaaatcta aaaggataat ttatgaaaga aatcttagta 4560 ttcactacta atgtacttgg acagcataac aatgctgctg ccaaattagc ttataagaaa 4620 cacggtgctc gctggggcat ggcctatggt cattatggta atagctttgc cattccagta 4680 aaggatggtt atggtaaccg tattaaagaa ggtgctatct atggcttcat tgaaggcttt 4740 attgcatatg catcttctaa tccacaatgg gatttcaaag taatgggaga taactttctg 4800 gacccatacc tgttcaataa tgtcactggc aatgtgctat tgccagaagc atggcataaa 4860 tacttaggta atgcttacaa ctattggagt taataatgac ttatgaagaa ctatggtctg 4920 ctcaagtcag agccagagca ctcacacgac acgatatcta ttgtgcatta cagaacgaat 4980 taaagagtcg tactaagctg ggtcacatat ccggcttagt taaaatacct atgactagcc 5040 tagtctggcc ttatcagaag aaaagtaatg agtttaatgg taatggtctg catgtacgca 5100 tcgactacat cggtaatgaa aacagtattc gtattacctt ttggactaag cgttagttaa 5160 tggctgctcc gcagcttttg gttattaatc acatttagga gaataagcga tgtactcatc 5220 cattgaacat cagaagcaac tcgaaaaact attcaataaa aatcagctct tgcctcgcat 5280 gaggaaggaa ttcgaggact ccgaagaaat tgactttaag gcattcgctg cctatttaga 5340 aattgattat aagctgctca ttgatgcaat ggtgcagatt gccctacata aacgtgctga 5400 catccaaact atgattggtt ctctaatgag tcactcagat gatgctcagt acatcgctga 5460 ctgtctgtat aagatggcag agaatgactg cttcaactat gaccctacca tcgacaagtt 5520 cggtgttatc tatgagatta gtgaagatgt acagatggaa ctggaagcat tccagtaccc 5580 attacctatc gtaagtgctc ctaagcctgt taagtgtaac cgtgatactg gttactacga 5640 aagcagaggt agcatcatac ttaagaagaa tcatcatgag atggatgtct gtcttgatca 5700 catcaaccgc atgaacaatc aacgtctctg cattaactgg gacgttgcta actacgtaaa 5760 agactcccgt cctaacatgg ataaacccaa agagggtgaa acccgtcagg actatgagaa 5820 gcgtgttaaa gcctttgaga agtacagccg tacagctaag gaagtaatgg agttagtgac 5880 caaagaaggt aataacttct cactggctca caaatatgac aaacgtgggc gtacatatgc 5940 ctgtggctac cacatcaact atcagggaac cagttacaac aaagctgtac tggagttcgc 6000 tgataaggag ttagtaaatg aagactaaca ttggctcata tgagctatgg gttaatgaag 6060 aatgcgttta ctccggtaca tatgtgaagt gcttatactt tgaaaagctt tacaagcttc 6120 aaaaccctga gagtaaaacc attatttata aactatctgc cgacgtagta actggctaat 6180 ccatccaata agcaggctct ccatagtggg gagccaagaa gcaaaaggaa cataaaatgc 6240 aactgttaac cgctaaacaa tacctgaaag tagatatcgc caataactat ggtctggata 6300 aaaagacatg ggatgagcgt attgcctggt tcgatgagaa cgaagctaac ctgcttaatc 6360 tggtagatga agctgaagat tctgcactgt tctatgctgg tgtaaatgca tggaaagata 6420 tgaaagcagg taagcctatt ggttatgccg tagctctgga tgctacatca tctggcttgc 6480 aattactggc ttgtctgacg ggtgaccgct ctgctgctga actgtgtaac gtagttaatt 6540 acatgggtga gaatggtaag ccattacgtc gtgatgctta tacagtcatc tatcacaaga 6600 tgttggacat ccttggtgaa gcatctcgta ttaaacgtag tgacactaag caggcagtta 6660 tgactgcgtt ctacggctca gaagccaagc ctaaagaagt attcggtgaa ggtattcgtc 6720 tcaagacttt tgagaatgta atggaaactg ttgctagtgg tccttgggca ttgaacaagt 6780 tcttactgca atgtggtaat ccagatgcca accgttacat atggatactt cctgacaact 6840 tccacgctgt cattaaggtc atggttccag aagtacagac tgttaacttc ttaggcaaac 6900 cgttcgacat tactcgtatg gttcagggta ctgaagagaa gactcgtatg ctctctgcta 6960 acatcaccca ctctattgat gggatggtgg tacgtgagat gctgcgtcgt tgtaacttcg 7020 accgtgactt agtggaagct gtgcgagaac tctgcgatga aggtccatca gaatatgggg 7080 agattgcagg taatctggag aaggtacaag agctatggag ccattatgag aagtctggct 7140 tcttatcttt atccatcctg gactatcttg acccatgtac tattgcttat gtagaccgtc 7200 aggtagtagc agatatgatt gacactctgc ctaagaaacc attccctgta atgactgtac 7260 atgactgctt ccgttgccat cctaactacg gtaatgacct gcgtcgtcag tacaatcaga 7320 tcctgtctga tattgctaag agtgacctac tgggcttcat cctgtctcag gtactggggc 7380 aagagttctc tgctggtaag ctggatgaca gcctctggca ggacattctt gaaacagact 7440 atgcgttgag ttaataaact agcctcattc cttcgggagt gaggcatatt ttttgcttgg 7500 aggtaacaac tatgctaatc ccttttgaag taatctctca gttaatactg atagttttat 7560 ctgtactggt tctgtctaca atagttcatg cccacaaatc taagtttgct tattactact 7620 atggagcata ctcactaggc tgctttgtaa tggcaggatg gatagctttt gctatctact 7680 gtgtaaacaa ctaaccctcc taacggaggg tttatttttt tacatgtcat gtatactaac 7740 tctgctattt acttatgagg aaattgatat gccaactttg aaagtaggtt ttaacaaaac 7800 cactaatgtt gcaactgtac tggatgccag tggttctatc ccaggtggtt ccgtagaagt 7860 aggaaccttt gtacacccag atgccactta ccctgatagc ttagttatct tccatggtgt 7920 tcgtgacctg ctgtataaac gctctgctaa agatccttct aaagaaggct tctggcctaa 7980 caacatcgtg gatatgcagt ctatctctat tgatatgaaa gctactccac gactgactat 8040 tgctaccaaa ctgcctcgtg tagtctctac tatcgaaggt gaagacatca actggcacgt 8100 tgatgtagca ggtgggaaag caccatttac ttataaatgg caattcaaag ctgatactgc 8160 tggagcagca ttcgctgata ttgattcagg tgagaacgaa tccgctgcta ctgcaacact 8220 gactcttaat gacgtaacag ctacttctgc tggtacttac aaagtgattg ttactgatgc 8280 taatggaacc acggtagagg atgagtcact attagctgta ggctattacg aagcaagttc 8340 actggtagct actcctgatt cgctggctct gtctgttgct gctgatacaa ctgctggtaa 8400 gactgtaaca attgttgcta tgcctgttgg ttcatcttct ggtgctttat ctatcaagac 8460 tgctccagat tctggtcgtg ctacggctac cattgctggt aacgtactga cagttaagcc 8520 agtggctgct ggtgatacta cttctgtagt agttaccaat ggtacggtag atataactat 8580 ccctgttact gttgcagaat aagggtattc tttgtttggg gttataaacc ctgagataaa 8640 accctcaagt ttgacctccc tattgaaggg aggtcttttt ttgacttaag catcctggag 8700 ccaggaaatg tacaaagaaa ttgcattctt ctcattctta ttaggtggtt taataggtgc 8760 aggatttgta gctatttcta atacctactt tggttctact ccaaattctg taacgcaaac 8820 cctcaagcat gaatgtgaat tgaatattcc tcgtaatcaa aactgtgtaa tgcagttcgt 8880 accggagaag aaatgaatat ccacaatcta catttggttg agaaactgta tgaagaatac 8940 aacaacaacc gacaacaatt agctagtttg aagaagtcac cacatatggt gaaagtatca 9000 ttcaatggta cggatttagg accacaagcc cgtacaagaa tacttcctgg actactgtcc 9060 ttttatcaaa ggagggttgc tgttttagag aaacgctttg aacatttagg tgtagattta 9120 tcaccacttc cagatgaagg agaagaggga tgaaagtaac aaatcgttct gagaacaatg 9180 aggtaacttt cggtgatgtg gagcctgcta atggttttat ttataaccag acagtgtgtt 9240 taaagattca cttacctgat ggtaaacctg ctgctgttgc tgtagaaact ggtaaatcat 9300 tctgtctatc tgcaagtaca ttcgtaacac caataaacct ggaaggatat tacctttgaa 9360 attaacacag tcccaagcaa tcttccttcg tatggttcaa ggtggctctg ccaccagcaa 9420 ccgtaataac aaaaccgcac agtctcttaa gaagctgggc ttagtacagt tcaatgctgg 9480 tcttgggtgg tcattaaccc ctatcggtgt acttaaactc aatgaattaa aaggtaacta 9540 aatgaaaact ttatttaaag gtattgcagt agctgcactg atagctttgg ctccaaatgc 9600 acaagctatt gaaccagaac caattttgga aggtgctcag gcttatctgg acaacaccca 9660 tgatgagttc ggacaaggat tcttcatggg cagtatgatc tcttacatcg agaacacaaa 9720 taactgtgtt ccagatggta tgaagtattc agacattcta cctaagattg ccaaagtagt 9780 tatttatgac tctgcaatcc tcaaactgaa gaatacatct caaattgttg tgtactcagt 9840 acacaaggcg tatccctgca ctaaatctta atcagtaatc ccattagctt taggagagta 9900 agtaaatggc aagcattgac tccttgactg tatgtaattc acgtcaagct cgtaacttta 9960 ttattcgtgc cctgaaagca ggtaacgtac cattcctgac ttcatcacct ggcatgggta 10020 aatctgcaat cattcgttct attgcagaag aatttggtat gaagttgata gatcatcgtc 10080 tgtctacttc tgccccagaa gatctttctg gtctaccatt ccgtaatggt gaccgtgcag 10140 agtttattcc attcgctgat ttattcccta ttgaagggga tgaagtacca gaaggttata 10200 atggctggct cctgttcctt gatgagttca actcagctaa gaaagaagta gtagctgctg 10260 catacaaact aattctcgac cgtatgactg gtcagaaaaa acttcatcct aatgtgatga 10320 ttgtctgtgc tggtaacaaa gctactgacc gtgccattgt taatcctctg ggtactgcaa 10380 tgcagtctcg tgtggttcac tttgaaatgg aacttaactt cgacatcttt gttgaagatg 10440 taatgattcc tcaacaatgg gatgaacgtc tggttgcatt tctacatgct aacccaggtt 10500 atctgcatga cttcgaccca gcccataaga acaaaacatt ctgttgccct cgtacttggg 10560 actttgttaa caaagacctt aagaatcagc cggaaggtgc tcttcctgat gaagattcta 10620 tctactatgc aggtcacgtt actgctggta aggctacaga atttgttcag tttacccagg 10680 tatataatcg tatgattacg attgagaagg tagtcaaaga ccctatgggt tgtgcactac 10740 cagaagataa caacctgtgt tgggctaccg ttaaccatct agctaacaaa actactgaag 10800 agaacttcgc tgatgttctt cagtacatcg aacgctttaa aacgttcacc cataagattc 10860 tgtacttccg tacagtaggc agaacattac cagaacttca ggctactcct gaatggcgta 10920 aggctgctgc taatatctct cgttacattc acggataaaa caatgaacca atttcctcag 10980 cacacactta gtgatgaaca actcatgcgc gaatatgacc gtattcaggc gcaggcgttt 11040 ctcggacgca gtgctgcctt ctttggttca ttactatgta gtcttaaatt ctcatggaaa 11100 cgtgaggatt gtcccactgc atgtactgat gggatagaac tccatttcaa cccagacttc 11160 tttatctgga tgtgtccaga tgcaagggaa acagtattaa tgcatgaact atggcatgtg 11220 gcatatctac atgacatccg tcgtggaagc cgtgacccgg aagtctggaa ccaggcatgt 11280 gaccacttca ttaaccttca gttagaggag gatggttaca agttcactgg tattaatgaa 11340 ggcatttgca aagaccctca atataaagga tgggtcgaag aagacatcta cgatgacctg 11400 atgaagaacc ctcagaaaag gcagaagccg tcagggggtg ctggagcagg tcttgctggc 11460 gacatgaaat cccccacttc gggacagtcc cagggtgctg tcgtcaacaa cgtagtacgt 11520 gcaatgcaga gccagaagat ggctggtgga acaatgcccg gtaagggtgc tggtcgtatg 11580 gaagaggtta ttacccaatt ccttaaacca gtggttccat ggcaagaagt actcatgaac 11640 ttctttaccg acattgatga cactcactat acgtgggcca gacctaaccg tcgttacact 11700 gacatttatc taccttccct ggaagatgat gaaggacgtc tacgacacct agcctacttt 11760 gaggatgtat ctggttctat tagtagtgct gactctctgc gttttaactc agaggttgcc 11820 tacgttaaga gtcaattcaa tcctaagaag atgaccctaa tcaccttcga cgatgttatc 11880 caggaagaaa tagacatcac tgaagaagat actttcgaag agattaagat tactggacgt 11940 ggtggtacaa acctggaacc agtacgcgaa tggattatta agaataagcc aaccgctgca 12000 atcatattct ctgatatgta tgttcgtcca atggaagaat tgccatttga tattcctatc 12060 atctggtgtg ttctgaataa tcctaatgct accgtacctt ttggggaggt agttcatatc 12120 cctaaaggaa tgaaataatg gttgttaatg gtaattctct atatcgttca tctcagttgc 12180 tggatgtccc agaccgtaag atatccgagc atggtgtaag ctatggatta ggtgaagctg 12240 gttatgatat tcgtatcaaa caggatatta ccttctatcg cttatttggg ttgattccaa 12300 tggtgaaagt cgttgatgga ggtcaagtat cacgccatct aggtaagttc gcgttggcat 12360 ctgcaattga gaagttcaac atgtccccat cctgtgtagc tatcgttcac gataagtcta 12420 catgggcaag acgtgcatta tctgtgttca ataccgtaat agaaccagga tggaaagggt 12480 atttgactct tgaactggtc tatcatggtc gtaagaaact acatatcccg gctggtgctg 12540 ggatagctca agtattattt catctggttc aggaacctgc taattacaat ggcaagtata 12600 tgaaccagga aaacaaacca gtggctgcta gaccttcaaa ataaaaggct atccggctta 12660 atcaaggaaa caacatgtca gtatttcaag taactcatgc aacctcaaat ctacgtttaa 12720 ccattaatgc tgcaaatgta gttgcagtgg aacagattcc actgtctgat tgtgcacgta 12780 ttacgcttgt aaatgaaggt actttggata ccaaagaaag ctatcgttca gtacgcaatt 12840 acctgaaaaa agctcttgct cctgcaagca aagacgctga gtaagtagct gcctaaatag 12900 cccagcatag ttgggctatt tgagaagaaa ctaactcaac ctcacatagg aaccatcatg 12960 gaatctttag cagcaatcct tgttttctta ttcatattag ctgtatacct tatccctact 13020 atcattgctt ttgcacgagg ccatgcctct aagtggggta ttggtgtact caatatcgta 13080 ttaggctggt cattagtatt ctgggtagta gcactgattt gggcactgtc taataaaggt 13140 cagaatcaag ttacaaacgt aactgttgtt caaaccaata gtggcagtaa aacagagtaa 13200 ctaacctaag catcattgca tagtggtgct tgtggaagtt acttccagct cattgcttat 13260 ccatctccta acccaagccc acctaacccg tgggcttttt tatttacagg tactcttatg 13320 tcaagcagac aaaatggtaa gtctatcctg caaggactgg acttaagtaa gctggaacaa 13380 actgcaatgc ttactttagg taaaaccata catgaccaag tggaaatgga tgggattagt 13440 tctgatgtct atgcaaaaat gcaggctctt attcagaaag gatggcccag cagagtattt 13500 aatataccta catacattcc cccagaaccc catctgaaat ctaaggtaga ccgaatcata 13560 gataagttct ggcttaaccc atgtggagat gacatgcatc tttacctggc tcaaatacaa 13620 aagaatcctc gtactaagga tgtgtttaaa agtaagagca ctacccatca ctacccatgg 13680 tatagaaggg gtagtaaata ctaatgcgta tcccattcct aagaaaaagg gaacgaaatc 13740 ctgttcttta taataaaggc atagaggatg agtatgaact caaccgtaaa gctcgttcct 13800 acacaacaaa actattcatg gggactaaca accctgatag attattggac tttgtttttg 13860 aacaagtctt tatcatatac agcttagcta tgtctgctgg ttcacgagaa gtaagtgata 13920 aggcaagaca tgctctctgt atgctccgta aagagtatga agccctcatg tatgaagact 13980 tgtactcctt taaagaagaa accgctgtag catgttctgt ggctcttact gaaggtgtag 14040 cagtattaca agaactaccc cgtagtgagt ttaagctggt atacgttcag gttaagagaa 14100 tcacagagac aagaagtggt atcactaact atctaaggtc attctaatga ttaaggcatc 14160 agtaattgca gattccgttc atccagaaac aggaacccgt atcacaacct ttgaattggt 14220 ttatccccga ttcattcaca gtgagtttat gactcaccgt gtattcaacc gcaatgcttc 14280 aagtagccgt gctattccta cctctaagtt aatcgaacag gttcgcaatg aaccagtgat 14340 gccaagtcac tggggcaaga accagaaagg tatgcaagca gatggagaac tcactcctat 14400 ggagattgag gatgctaagt ttatctggga taacgctgca tctgctgctg ctgtgtatgc 14460 tgaacagcta cgccgtgggc aagtacataa acagattgtt aaccgtattc tggaaccctt 14520 cacacatatc cgtgtagtgg taacctcaac tagctgggct aatttctatg gactgcgtga 14580 ccacaaagat gcacaaccgg agattcgtga actggctcaa gcaatgcgta aggcacatga 14640 agaaagcaca ccaagagcat taccgtatgg gcaatggcat ttgccatata ttgctcatat 14700 tgaccgtgtt ggggcttaca atttctgcaa acgtaatcga attacacgcg atgaaccaag 14760 tgatgcagaa gtgcatggac tacttctcaa ggtaagtgct gcacgctgtg ctcgtgcttc 14820 ctataacaac tttgagggac gtccctctac tatcgaagaa gatcttggct tattcgctaa 14880 gttagtggaa aaccaaccta ttcatgcttc accaacggaa catcaagcta cgcctatgaa 14940 ccttggtgag aagtatgtga ataacatgaa cccagttacc tgggaacaag gtgttacatc 15000 catggataaa gaagggaatc tgtactcagg taacctgctc cactttatcc aattccgtaa 15060 attaattcct ggtgagacta ttactgaatg aaaaaactag ctctatacgc aatgctaatt 15120 agtaccctac taacactgac ctacgcatac aaagttgcct ttgtcgtaga aacagatatg 15180 cagttcattc gagctagtat tctgttcttt gtaagcgaga ttggtttatg gtgtgtttac 15240 tactttgctc gtgactacga agcaattcgt gagcaagaag aagtgaaaaa acaaatgata 15300 cgatttgtag aacaaaatcg taagtaaacc taagacctcc tccgggaggt ctttttttgg 15360 tttcaattaa ctctttcagg agggcatatg cctgctaaat accgtatcaa agacacaccc 15420 gtaatgtgtg agggtgagaa gggcgacatt gtatatgcct gtatccagga tgatttcaat 15480 gctgctcaga tgctaaccca aatgacaaat acactgcatg tgtcagtaac actggaccct 15540 accggtgact atccatgctt ccctattcct gcccataacc tggagcaaat ccatgattaa 15600 ccctgaagta attcatagta aaaccggtaa ggctgtccca ctcagtgaga ttgcagtaac 15660 tggtgatatt gctgcttgtc cagctaacat tgcctctcta tgcatatgca tcgctgcact 15720 agcagaggaa cgtaagttat ggctggaacc aagcaaggaa atgattcagg ctggtttagc 15780 tgaagtacaa aacacgttag ataactggga agagaacggc ccactaccat atggaacggt 15840 caacgatata acagatgaca tggcatcaga tatggctgtg tttgtattac aagcaatggc 15900 aggtaaacgc aatggctaat gtaaatatcg cctcagagaa aacgtatagc attcagatta 15960 atggtttaac cgagtatcag gtcttattcc taatgaatgc ttttcaaaat agtcctgtag 16020 gtcaccatcc taatgatgaa ccacgggaag aagctgaact tcgtaaagct atttttgata 16080 agtgtaaaca agttctaatg taagcaacat ttaatctgga gagtaaataa atgttagttg 16140 cagataccaa cgaaatagct acctcagcga cactgggtgg caaagaaaca attgcctttg 16200 gcatctcaga tgactcggca ttcttccatg tattaagtac ttccctgtat aacaatccta 16260 ctctggcagt agttcgtgag actatatgta acagctggga tgctcatatt gaggcaggta 16320 aaactgatac ccctattcgt atcaccattg atacagacaa ctttattacc ttccgtgatt 16380 acggtagtgg tattccagat gaactcattg gttccattta tggtgtctat ggtgcatcta 16440 ctaagaaagc caacattagt gttactggtg gctttggtct gggatgtaaa tctccattcg 16500 cttatacaga tagcttccag gttacttcat ggaaccaagg aaagatgtct gtatataacg 16560 tagctaaggc tgcgattgag aatgatggta agccgggtat tgtccctatt gttaccaata 16620 tacctactga ggaatccggt ctggaagtta aattccagtt aggcaaacat gatttaaata 16680 cctttattca ttacatcaag tcaatcgtat ttaacggtga gattaaagct gagcttagta 16740 tccctaaact cgttaaaaca gaagaaggta atagtattca acaaggtgac tacactttac 16800 tgaatacgct gggcatgtca tttgaacctg gttcatatga tatgtctgat agatggtatc 16860 agggctatat gggtagcagt aacatatacg ttcgctacgg taatgtaatg tacccaattg 16920 tatccagccc agctagtgaa gaagctgtag gtcttatcct caacttcatg aatattattg 16980 gtgctggcaa tttagtagtt caggctgcac cagacacctt agctattgct cctagtcgag 17040 aaacactgtc taaccagaag ttaaccgacg atggtattac tactctatgc gtagatttag 17100 tagaccgtat ggagaaagag attaaggcta agattcctga agccattaag caggttgaag 17160 aatatgcctc taaatcctct actcgctttt gggaatatcc atctttcttg ggtgctgtta 17220 cagatagaac tgttcaacgc tatatgtctt ctagtttatg gactaaacaa cgtaagcatc 17280 acataaagca ctggcgtaac ttatccaata aggcgttatt agctcgtcct gaatatgcag 17340 gtcttaagaa gctgtatggt aaggctatgc atgctcttaa ggatactcgt gaggaaagta 17400 catactcccc attctcagaa ttagtatatc gccatctgca tttacctcgg cttgctgctt 17460 taaaaacctc tggtattaag tggtctggtt acataatgaa tcaaggcaac cgtgttgact 17520 tggtaaaagg taaacttact gactacttta ggatatacaa taactctcac caaagtattg 17580 gcatatttac taccaagaac gttgtagtta cccgacgttt atctgactgt gcagactctt 17640 tctcatactt ccctgaatac aatcgaggtg acctggagcg tacagctttt gttcatgtcg 17700 ttggtcctaa gaaaggtgca gcagaggaag ctgtagctaa gttcactgct atgggttatc 17760 gggtgattga ccttactcaa tataatgagt gggacaaacc aactaacttc cgcagggaac 17820 aagctaagat agctgctgag aaacgagcca agacaatagc tgccaataaa accaaagcag 17880 gtggtaaaac caatgctctg atttcattga atgcagttct tggtgctacc caggtacgta 17940 ataaaaatgg ggattgggaa cccaggccgt atattcagaa agaatttgct gacccaagtc 18000 gtcatgagaa atatggtttc gttgagatag aacagcctaa gtactacgta ctagccaatc 18060 aggttggttc tggtagtcct gtaactgctc gaattggaac catgtggaaa tggtatgagt 18120 tgcctgatga gatgaaagca gagactgttg tctgtcgtaa tcaaatcgaa gctaacaagg 18180 ccaaacgacg aggtgctatc cacattgatg atgttcgctt tagtgaattg atgtctgtta 18240 ttaccagcaa aggcttcaag aaatatgtta ctgaacatcg catcggtatt cttgaatacg 18300 taggactgga tgacagggaa tactgggaaa ttctagacat actgggtcta accttcaaac 18360 ccttacagaa tctgattttc aaaccagagt atgaatgggc atatgacttt ttacgaaatc 18420 gaccacacga caacaaagaa aaacttgttg aaatgggttg tattaagtca gttgatgact 18480 tagagccata tgtaaaactg gttaatcccc gtaaccataa gtactttaaa gtacttaatg 18540 attacaaaga attattcagt tatagctgga ataaaaatga catcctacag acgctggact 18600 taggtagttt ggtcaaacac cttaagaaga atccagagga tattcctggg tttaagtccc 18660 tctaccgtaa tcgtctaaat aaactgaaag gtaactaatc tgatgaaaat cgaactgatt 18720 tctatcatcg cactggcagt tgacagtcgt aaccttacat tgtggaagcc cgatggctcc 18780 acgattgtat atcctcagag cgacccacgg gttgctcgca ttgtatctga agctcaaact 18840 aaaggtctgg gaactaccaa agaccaaata gaagtaaaca tcgcaccaga agtaaacctg 18900 cgtactgaat atctggaagc agagaagaac actaacggat tcgtccgttt cttcaaggta 18960 gctaaagcta aactcaaaga gttctttcag gatggtacag gtgttccacc tgaccgtatt 19020 atttctgata ttaagctggg taatcctact aagacactgg tgtctaaagc tatggatacc 19080 tttctggctg tacaggccaa tgaaccagaa gtaactgtaa cagatggttg ttatgacaaa 19140 cgtgacaatc tgatgtgggt tactggctgg gacaaagacc ataaccatcc tgcactggtt 19200 cgttttatta acgacgtaat gggttgtggg tatgaatata ctaacaccat gctcaaacag 19260 gaatggcctg ttgcagttcg tgcagtatct gatgatgaaa tgggtgagtt tgctaaacaa 19320 gccgaacaca tcaaaggggt tcatgttgta ttcaccagtc gtaaacatac cccaccacct 19380 tacattgaag taactaaagt tacgaaccag gataagctgg ctgctgcctc ggagaaacta 19440 gctgcattgg gtgctattag tactgatgac gctaacttcc acaccgatgt gaaggaagat 19500 gaagtggttg ttgctgttac caacaatggg gttatccccg gagttgagaa cctgcaacgt 19560 cacttacgtc agtctgccaa gctgaaagac tacaagggct ttactaagtt ccttgaacgt 19620 ctggctccgg ttattaaaga ccgtctgcac tcagtagaag acctgatgaa gttcatggaa 19680 actgctgaac tgcctattgc cgatgatggt tcaatcctgt tcctcaaacg ccttaagtct 19740 agtggtatag aaaacggtaa acgtgtattc gttgactgtc actcgggtaa cattcgtcaa 19800 tgggtaggct gtaaagtgca ggtacgcgaa gacctggtag accctgaccg tcgtcaggat 19860 tgctctaacg gtctgcacgt agcatccatg agctatctac gtggcttcgg tggtaatgtg 19920 accatccttg gtaaagtagc accggaagat gtatttgctg ttcctcagta cagcaccaat 19980 aaaatgcgtg tatctgcata tcatattatt gctgaactac cggaagagga acgtaataat 20040 gttaataatg gtatctacct gtctaagaca gaagtaggta agaaattgct taatgatgcc 20100 atcgttggga accatagctc acctaccaca cttattatgg ttgggggtca ttatggtact 20160 aacctcaaat acactaatct cacatctggt tctgtagaac aattccgtac agttgctagc 20220 aaagaagcac tgaacatgga agagtcactg aatgaagctg tagctgctga accagtgaag 20280 gctactgacc ttaaacctgt tattaagaag gctcctactg tgaaagaaca aatccaggaa 20340 ctggtcaaag agttccttac tgcaacaacc ccagaagata agttagctgc tgctgacctt 20400 ctggtagaac tgcgtggtaa agctcgtaag ccttgggctg cattcgatgt gggtaatgat 20460 gtagtagcta agattgctga tgtacgtgct acctatacag ctaagcctat tggtaaacct 20520 aaagctgtta agcaggataa gacagttaaa cctactaaat ctaagcctgc tattaatagt 20580 actaatgcca acatcatcag aggttatcta gcagatagtg gtatgtctga ttatcagaaa 20640 gcccactcca ttcatgacct gaaacgtgca gctaagaagt cttatgctgc tatgggtctt 20700 actgaagaag agtgcaaagc cattgataag ctgaagcacc accttaagta atagcctgtt 20760 caaatagcct cacttataat aaagtgaggc tatctttgaa gaggaaataa gctatgtcta 20820 aagtattcag aagtaatcgc aaagcaactg atgaagacat cattcgtatg aatgctgttg 20880 gtctgtccct cgcaactatc gctaagacgt tgggggttca cccaaccaca gtcactttgc 20940 gattgcgttc tctaaacatt gaaccagccg acacacgtcg gacgttcatg gaaaacgtat 21000 tacgaccttt accaacccat gtggctgatt ggctgtcaga acaagttggt cctgcttatg 21060 agattcgctc atatgtaaga gacttgattc tggaggcata taataatcgc caccttaacc 21120 aagagagtga gcatgacaag ttcatccgtt tgtacgctgg caaatacgga agcctggttc 21180 cggaaagcag tagcaaatcc aacaagtaag aatattagta cccagattgg ttgtcatctg 21240 gaagaagtat gtgaaatgtt agatgagttg accccatcct gtgaggatag tatgcaagta 21300 ttagccgatg cacaggctgc tttgagtaaa ttatctctgc tgatgaaaac aaatcacatg 21360 gctattttcc cagcgaatag caatcgctta gctttgctgg attcactggc agaccagatt 21420 gttacagcaa ctggagtcgg tacttttctt gggatgaatg tccctggagc attggctgaa 21480 gtcaatcgct caaactattc taagtttgaa gatggagaac ctgtcttcaa tgggaacatg 21540 aaagttatga aagggaaaga ctacactccc ccggatttaa ccccttacat ctaaccctct 21600 acggagggtt ttttactgga gattttaatg ttttccaaac ctaccaaagc cccactgaac 21660 aaggggcaag aagcggttgc gaaggagttc ttcgacttcc tgctcgaccc taatgctacc 21720 gaattcaata ttagtggccc agggggaact ggcaagacat tcctgatgtc gcacctcatt 21780 gatgacacta tgcctgcata tatggaaact tgctccctta tgggaaccaa gcccctatat 21840 aacgaagttg ttatgactgc gaccacgaac aaggctgctg aagttctggc tcaagctact 21900 gggcgtccaa catctaccta tcattccttc cagggattga ttgttaagaa tgactttaag 21960 actggtgagg ctaatgtcgt accgtccaaa tcattcaata ttaagaagaa caaaatcatc 22020 ttcgtagacg aagcatccat gattgaccgt cagttactta agtatgctcg tgaaggtact 22080 caccagtgca aactggtatt tgtaggcgat gcttctcagc ttctgcctgt taaagagaat 22140 aagtctccag tgtatgcagg taatatccca acacactatc tgactgaaca gatgcgtacc 22200 gatgcaccgg aacttaaagc attgcaccag caattgcgtg atacggtaga aggtaagaca 22260 ggcttcctgc ctattaaatg tattccaggc atcattgatt gggtacaggg ggaagagatg 22320 gagaaactgg ttctcagtca cttcactcaa cctactaata gccgtattgt tgcttacaca 22380 aatgaccagg ttattaatta caataactac attcgtgaag ctaatggcta cgtgggtgag 22440 tactccattg gtgaacagct agtctctaac tctgctattc gcttaggtgt ggataatcgt 22500 ctgtctatcg agcaagacgt aaaactcatt gaccaggata gcagtactcg catgattcca 22560 gttacagatg acctggaact ggaagttcgt gatagtactc tggaccttgg ttatggtggt 22620 attgtaagtg aagtcccagt acctaccgac ccagaatact tcaaccgttt ggttaagtgg 22680 ctaggtaaag agaagaactg ggaaccctac ttccgtctta aagaaaccat tccagaccta 22740 cgtgctactc atgcatgtac tgtccataaa tcacaaggct ctacttacga cacaatcttc 22800 attgatgcag atgacctctc aagctgtcgc caacctgata tggttgcccg tctgctttac 22860 gtcgctgtgt ctcgtgcccg taagcgagta gtgttttatg gcaatcttgc cagtaagtat 22920 ggtggtctaa ctttctaagg gaggatatat gcctcagatt ggttcagcga ctattggtca 22980 ggttgccaat agcagtgaga tagtcaaaca cctgttctta gcagaactgg ttcgtcttga 23040 tagtgtgtta aacggtatca ttgataagaa cgaccgtatc aatggtattg atatatcggc 23100 tggatttctt tatcaagggg agttctatca gcgttctaat gctgccagac ctccaaccta 23160 cggtgaacga ttaacactta atccagaact ttggcctgca atggacaagt atctgaaagc 23220 ctccagtcgc ctgattatgg aagtacacct tgtgaaccag actgtatatc gcctggttcg 23280 tggttgtatg tcctatcagg atgtacgtga tgctttacct gaatgcctgg tagcccagga 23340 ccagactggt aagtacaagg aactgccacg tactcgtgaa gcagcctgga cacttgctgg 23400 tgatcctatg gcaataaaac agtatgagaa gattcttccc tctattgagt actatgcagc 23460 ttcccatctg attttctaag gtaaggctat gcgttacatc acctctcagg atacgggtaa 23520 gtatcctatt gctatcctcg gtcatcaaat ccgaagggag gagatgatta aaacctacct 23580 gctgcctaat gacctaagca tggaagattt catcttcatc gaacttcatt ctgcccccgg 23640 caagaagaag actcctgcaa gggagattaa ggagttcata cagcaggagt tgcaacaagt 23700 actggacgat gcagagactc aatacattat ctgtaccgat tctgactact tcaaaatact 23760 gactaaagaa gcaaaagcag aggctaacct cggctacgtt tgtgattcag tatggggtaa 23820 gcagaaggtt atctatgcac ctagctacag acaggtcttt tatgaccctc ctgtagtgaa 23880 atctaagatt gctcagggta tggatgcatt acttaaccac atacgtgggc agtatgcaga 23940 accaggtcag ggaatcattg agtttgaggc ttatccagat accccagaga agattaaagc 24000 ctggctagac cagttgcttg agatgaataa gccattggct atagacatcg aggcattcgg 24060 tttaaagcac tataacgcag gtataggaac aattacgttc tgttggagta agacacaagg 24120 catagccttt aatgtggact acgagccgat tcctggagct actgaagcac catacgggcg 24180 tatcaacaga aatgatgttg ttcgaaatct tcttcgtgag ttcttcatta agtacactca 24240 acggcagatg tatcacaaca ttagctacga cgtgtatgtg cttatctatc aattattcat 24300 ggataacctg attgatacag aaggcttact gcatggtatg gaaatcatgc tacgcaactg 24360 ggactgtact aagttaatca cctacctggc tactaacagt tgtgctggta atcaccttag 24420 tctaaaagac caggctcagg agtatgctgg taactatgct caggatgaca ttaaagacat 24480 tcgtcttatt cctaatgagc aactcttacg ttacaacctc attgatggtt tatgtacctg 24540 gtacacctat gagaaacact gggatactct cattgctgat gaccaactag atgtttacaa 24600 caacatcttt aagccagcct gtgaagatat tatccagatg cagttaactg gtatgcccat 24660 gaatatggat accgttaacc aagtagctaa ggagatggaa actgacagga accaggctct 24720 gaaaactatt cgtgagtcta agctcatgaa gaactttacc ctgatgcttc gtcaggaatg 24780 ggtagatgat aagaatgcta agctcaagaa gaagcaggta acacttgctg actgtgatat 24840 cgagtttaat cctaactccg gtccacaact acagaagcta ttatttgact atattggctt 24900 accggttctt ggtcttacta agagcaagca acctgctact gacggtgaca ctattaaagc 24960 actgcgtaca cacacgcaga gcgaagatgt taaggaactg ctcaatgcac ttatcgacta 25020 taagctcgtg gataagatta tcacttcatt catcccggct ttccgtaatg cacaaccggg 25080 accagatgga tggcactacc tattcggcaa cctcaatctg gggggaacgg tttctggtag 25140 attatctgcc tctgagccaa acctgcaaac tattccgtct ggctccaaat acgccaagaa 25200 gattaagaaa tgcttcgaag cacccccagg ttggatcttt tgtggactgg actttgcaag 25260 ccttgaggac cgtatctcag ctttaactac taaagaccct aataaattgc gtgtgtatac 25320 tgacgggttc gatgggcact ccctcagagc taaattttat ttcggcgagc aaatgccgga 25380 tatagatgat tctgtggaaa gcatcaactc tattcagaaa aaatataaag ccttacgtag 25440 tgaatcgaaa gctcccactt tcttattgac ttacggtggg acttatatgg gcttgatgaa 25500 aaactgcggt ttcccggaag ggaaggctaa gttaatcgaa tccagatacc atgaaatgta 25560 tacggttagt gatgcctggg ttcaagctaa gctagacgat gctgccaaaa ctggttatgt 25620 tactgccgca ttcggtttga gagtgcgtac tcctttactg gctcaagtat tacgtgggac 25680 atgtaagact ccgtatgaag cagaagcaga aggcagaact gctggtaatg ctttagggca 25740 aagctggtgt ctactaaata accgtgctgg ttcagagttt atgcgtaaag tcagagccag 25800 tgagttcagg ttagatattc gtcctagtat tcatattcat gatgctcagt acttcatgat 25860 tcgtgacaac atggatactt tgcaattcac gaacaagcac ttggttgaag ccgttaactg 25920 gcaagaccat cctgatattg ctcacccaga agttggtttg ggtggggaac tatccttgtt 25980 ttacccaacg tgggctaacg agattgaaat tccaaatcac gctaccccag aagaagttca 26040 tcaaataatt caaaaggcat tcgcatgacc aaaagtacta aagaaactgt tgtcagaaaa 26100 tatcattgga tggtagcagc acaggtagta ttccaacttc ctaaagtgga tgatggttcc 26160 ctgcttacca tgaacacaat gttgctcaca gatgaacctt acgtgaccta taaagatttg 26220 gctcgtgcca atcactctct gaaaatcagt ctggaccagc gtttcgacac ttcagttgac 26280 ttgaaagaca tcgtttatct gtctcttagc aacctgggtc tgatgtctga accagagttc 26340 caggcaaaca tgattcccaa ggagaaataa tggctaagct ctccggtgga ttgaataact 26400 ggtatgtagt accagttaag caccctcaac ggaaagagca agagccatac caagcagagt 26460 gtgaggatat tatccaagca ctgggcatga ccttcgatga aggttgtgcc tttaaagctc 26520 tatggcgaaa tgctgctgcc cgtatgggta atggtaaacc tggaaacact gctgtttacg 26580 atgcagagaa gctggttcat tatgctaatc gtattcttgc taaggagaag ttagctagtg 26640 agttatttcc ggatcctgct acgaatgtaa ataccgttgg tagttattgg caccatacaa 26700 acaatggtaa acctcacttc actaaagaat tttcattcat tgagattgtt tataaagatg 26760 aacgagatga aatttactcg tataacttca atcagctaag tgaaattaag tggaactggg 26820 ttcacagata caggattact tactaatgaa gataaccaac aaccatgatg tatcactggc 26880 cctggctgta tggctattgc atgacgagta actaattatg aaacagagga ttgcccatga 26940 caggctttta cagcttgtta gttatgaccc tatttccggg atttttactc gtaggaatac 27000 cggaaaggta tctggttacc taatgaagag tggttacgtt caactccgtg tggatagtgt 27060 gttgtactat gggcatatcc ttgcatggtt ctatgtgcac ggtgtatggc ctacggatag 27120 aattgaccat aaggacaata ttcgccatca caactggata gataacctca gagaagcgac 27180 ccacaagcag aataaccaga gtgctgtttt atctaaaaca aacacatctg gatttaaggg 27240 ggtatccttt tcaaagaatt taggtaaata cagagcaact atttgggtta acagtaaacc 27300 aattacatta ggttttacag atgacccaag agaagctgct gttctctatg atgaagctgc 27360 tataactcat tatggtgagt ttgctaaaac taataagcaa ttgggactgt tatgaaactt 27420 accaacaaac acgacgttag tcttgcacta gctgtatggc ttgtaacgga tgattatgat 27480 tatgtagaca atcctaagta tctgtctgtt actaccttgc ttaagcccat taagcaaata 27540 gtcatgaagc atcgtgtaga tcttagtgac cagtcaattg atgttatgga tttcgtctcc 27600 acatcaatgg gtactggttt acatgattct atcgagaagg cctggaagct gggtcataag 27660 actgcattga aaaagttggg ttatcctcaa cgagtaattg atgcagtagt cattaaccca 27720 accaaagcag actttgatgc taaccctgac cttatcccaa tctacattga acagcgtgga 27780 accaggatag ttaagggttg gactatcggt ggtaagttcg acatcgtaac agaaggtctg 27840 ctgcaggact ttaagtctac ctcaacctat tcctgggttg ctggttcccg tgatgatgaa 27900 cataagatgc aaggcagctt gtatcgttgg attcacaacg acatcattac cgaagatgta 27960 atccgtatta actacatctt cactgacttc atgaaacaca tggctaatag caatccgaac 28020 tatcctgcta atcgtattat gcataaggat attccgttgc tatctgtcga gaaaactgaa 28080 cgttgggtag aagagaagat tcacctcatt gaaaagtact ggaatgcacc tgaagaggaa 28140 attcctgaat gtactgatga ggagttgtgg cgaacagagc cacagttcaa atacttctct 28200 gatgcttcta aggtagatgt acctggagcc agaagtacca aaaaatttga cgatatggca 28260 tctgctcgta tcttcatggc tgaaaaaggt ggcaagggtg ctatcaaggt cgtggagggg 28320 caggttaagc gttgtctata ctgccctgtc gcgtccattt gcaaacaaag agagagatat 28380 tttccatcat gagtattgac ctgaccggag tcactcacca ccctgcaatt gaagaaattg 28440 tagacgtgct gtgtaacaag acacaaaaca acgacagagg attcttccgt gtcgaagtag 28500 cctacttcct ggctaaaatg gcatcctgca tgggtgcaac cattgtcact aaagaccgtg 28560 gtgacttacc agtcaacatt tacgctatgg cattagcaac gtctggcttc ggtaaaggtc 28620 actcggtaaa tattattgaa gacggcttca tgactggctt ccgtaaacgt tttatggaag 28680 acaccatgcc cgtcattgca aatgaccgtt tatggaagat tgctaacgaa cgtgctgctc 28740 gacaaggtac agaccagaat gatgagtttg ataaagtcga agcagagtat aaacgtgctg 28800 gagcatatcc gtttacgttt gactctggta ctccaccagc agttaaacag ctacgacata 28860 agctgttaat ggctgggtgt ggttcaatca acctacagat tgatgaaatt ggttcaaacc 28920 tgttggctaa cacggatgta ttaactctgt tcctggaatt atatgaccag ggtaaggtta 28980 aacagaagtt aaccaagaac actgctgaaa gtgttcgtgg tgaagaactg gatggtaaga 29040 ctccagctaa cctgttgctg tttggcacgc caagtaagct actagatggt ggtcagaccg 29100 aagaccagtt ctatgacttt ctggatacag ggtatgcacg tcgttgctta tttgccattg 29160 ggcatttaga taaacgagca catgcaacaa tgtccccaga agaaatctac cgtaacctga 29220 ttaagcagga taacgtacag tctctgggta agtgggctaa tcacttccac agtctggctg 29280 atccaaactt gttcggcttt aagatggttg tagaagatgc tgtgggtatt gctctgatta 29340 cttacaagat cgattgtgag aaacaagcag aagctatggc tgaccacgaa gaaattcgta 29400 aggctgaaat ctcccaccgt tactttaaag ctcttaagct ggctggagca ctggcatttg 29460 ttgaccaaag ttcattcatt gaaatgtctc atcttaaaca agcaatcttg cttgtagaag 29520 aatccggggc agcattccag ggtattctca atcgtgagaa agcctatgtg aagctggcta 29580 agtatatcgc ttctgtaggt aaagaagtga ctcatgctga cttactggag tcgttgccgt 29640 tctataagag tggcaatgca gctcgtaatg agatgatgac tcttgctaca gcatggggat 29700 acaaacagca catcatcatt aagaaaactt ttaatgaagg tattgagttc ttccgtggag 29760 agactctgaa agagactgac atcaatgaga tgatagtggc ctatagtgat agctttgctt 29820 atgactacat tggtgaacgt gtaccgttcg accagttgca tgtattaacc caagctcccg 29880 gtatgcactg ggtaaaccat cacatgaaga acgggcatcg ttccgaagag aacgttattc 29940 caggatttaa catgattgtt attgactgtg atggtggagt accactgcat acgtgccatg 30000 aactgatgaa ggaatataag ttcatgacct ataccactaa acgtcattct gatgaagaga 30060 accgcttccg actgattatt ccaatgaact atgagttaca cctcgacact gaggaataca 30120 aagagtttat gaataacgtt atgtcttggc taccgttcga aacggatgaa tctgctaacc 30180 agcgagccaa gaaatggatg tcctgtgaga ctggttccta tcattacaat cttgaagcaa 30240 atctgttgga cgtgcgtgac tttattcctc gtactagtaa gaacgagcag ttccagaacc 30300 agatgaagga agtacagtcg ttggataatc tggagcgttg gttcgctagt cgtattgcta 30360 ccggtaatcg taataatcaa atgattaagt acgcactggc attggttgac agtggttggg 30420 attttgccca agtacagcaa gccgtccact cattcaataa gaaactggct aatccattac 30480 cagatgatga attgaatgca accgtaatgg tcaccgtggc taaacgcttc gctggcaaat 30540 aagcaaacag gagtctttct ttggtttgaa ggactcctaa attaaatgag gaaaaataat 30600 gtccgaagta attcccaatg atatgaacac tcagctaatc ctgattgcag gattctcagc 30660 gagtggtaaa tcagcatcac tgcgtaacat caggaaccag gaacgctggc tctatctgaa 30720 cactgaggca ggtaaacgtc tacctttccg taacaagttc aatacctaca acatcgaaga 30780 cccataccaa atctgggaag catttgatgt tgcatctcct ggtggagaaa tggcagatga 30840 tgttgatggt atcatcattg actcagcaac ttttatgatg gatatgctgg aatcccagta 30900 tgttctgcct tctgcaaaca cgcaaaaggc atggggggat tttgcacagt tctttaagat 30960 actgctgcaa caaaaagtcg ttaagtttgg taagccagta atcattactg ctcatgctaa 31020 agacgaactg gatgaagctg ctggtgtgat gaaaacgttc atcccagtga aaggctctct 31080 gaagaataac gggcttgaag cctacttctc tacagtggtt tacgcagaac gtgtagacat 31140 taaagaactg gagaagtatg gaaacaagat gcttgaaatt acggaggaag aacgtgattt 31200 aggctataaa catgtattcc agacccgtcc aaccaagaag tctgttggta aacgacttcg 31260 ttctcctatg ggtatgttcg ataagtccga gacttacatt gataacgatg cccagaaact 31320 cttagaccac ctggctgaat actacgctta agcgtttgtc tggttgttaa tcacttatta 31380 ggaaaatcat atgtcattgt tcagtaatct gaaagaaaaa accaaaaacg ttgaagctgc 31440 taaagactct ctaggtggtg gtggcttcgg tgcaaaagaa accgatatct acactggtac 31500 tgtaaaagta gcttacgtag gcaaagctga ttctggtgca gactggatgc agttaattat 31560 tgaagacctg aaaaactctg atggcgtgcc tgctggcgag ttccgtgctc aggtgtactt 31620 cacttctggt aatgctaaag gcaacaagcc gacttacgag aaaaatggta aagagtactt 31680 cctgcctggc tacactgtca ttaacgacat gatgctgatg gctactggta ctgaactgcc 31740 tgaagcagac ttcgaagaga agattgttaa agtctacgac tacgacgcta aagcagaagt 31800 taataaatct gtcatggttc cagttgacct ggtaggccag actgttacct tcgctctgga 31860 aaaagttctg gaaaacaagc aggttaaagg cgacaacggt tatgtagact ctgacgaaac 31920 tcgtgaagta aacgagattc agaaagtgtt tcacccggaa ctgctggtca cagtcgtcga 31980 ggctcaggaa gcagagaagg ctgaaaaaga actaacccca gaactggctg tattctatgc 32040 agcatggctg gaaaagaaca aaggcaaaac tcgtgacaag actaagagtt ctgctggtgg 32100 taatggtaaa ggtggcttgc ctcctaaacc aggtgcaggt gctggcacgg gtactgctcc 32160 tgctggtggt aaatcactgt ttggtaaacg ttaatgaaaa tcccaattgt cggtgcagac 32220 attagtctcc gcaattgggg tttagctcgt gggatgctgg acattgagtc cggcgtcttc 32280 gagcaggtcg aacttaaact ggttcaaact gaagttgacc acaacaaaca agttcgaacc 32340 aactccaaag atatacaagc cgctcacgat ttgtttcttg gttgtgagga atggttacgg 32400 tctgctaaag cagtattcgt agaagtacca gtaggctctc agtctgctaa cggtatgaaa 32460 tcctatggcg tatgcgtagg attaataggt gcatttcgtg cattgggttg tccaatcttt 32520 gaagtatccc caattgaaaa caaacttgca ctggtcggtg ataaaactgc atctaaggac 32580 acgatgattc gtgctgctca tgccatctat cctgaagcca actggctcac agataagaag 32640 ggcaaacttc tgaataagaa tgagcactta gctgatgcaa tcggtgcaat ccatgctggt 32700 gtaaatctcc cagctttcca aaacctcctt aatttaataa aggcataaca tgcaaatcat 32760 tttgaaccag tccgaagtag aagctgctgt acaggcttat gtcgatgatc aaatcaatct 32820 tgctggtgac atcaatattg tcatcaatgc agacggtaca gcttccgttg gtatcaacga 32880 agaggttcat gaagatactc cacccgtggg agtagagaag aaaactcgtc gttctcgtaa 32940 gaacccacag gaagctaaac atcgtccggt agaaccagag ccggaagaag tagttgaaga 33000 ggtaaaggta gaagaaaccc agacctctac tggtggtcag aacgagagtt ctacgccgga 33060 acctgaagaa gaagcagtat ctgaaccaga agcacaagaa gaagttgtgc aggaagaggc 33120 taaggctgaa gaaccagcag agaaacctgc tactaagcct tcactgttcg ctggccttaa 33180 acgtagttaa tctggtaggt ggctcagaag ctgctgctag gtgtggtagt gtttatagtc 33240 ctgatgctac tactggtcag gattatagac gtgtcggctc catacatagc ttttatcatc 33300 actgtcatta tcctgtggaa atgcagtggt aaacacggtg gtgacaagcc gccagagtaa 33360 caacaaccga accagtgtgc aggaatactc tcccctgtgc attggttcaa ctgaggctaa 33420 tatgaacaaa tttactattc actggctcaa tggaaaggtt agttctttca tgggtggaga 33480 acccgttgaa ggaaagaaag catttcacat tgaatcggaa ggatgcaaaa tcttagtacc 33540 gtatgcttgg tataaagaag gtgaagtaga agcattaaaa aagagtagtt aatatcgtga 33600 agggttctgt tagaatcctt tgagatagga actcctatca taacctttct tatcttaatc 33660 tttgccgtat ggcactgacg ctagggttta tgcccatccg tcaggtagac tattttaaga 33720 atgggttatc acagaaaatg taaagcaaca ttcgggttaa tcaatggcct ctccctatag 33780 gggccatttc tgaatgatag gctatatccc aaaggttagt atctttggga taaggcttca 33840 gatcccttat caaactaacc tctcaatgtt gagcatttta atcacataag cccctatatg 33900 ggctgggtta aatgtcggat agcatagcta cctacagcat cgaattgtta ggtcttacat 33960 gcaacatagg gtataaaggg aggactcgtc ccctccctcc tattataatg gtcgaggtag 34020 cacgtaggca tgtgcagatg gtcgctactt ctctcgctgg ttcgaatcca gcccccgacc 34080 tattgacatc ctatcaatga gtacattaag ctaacgtgcg gaggtttaca ctatagctgg 34140 tgtaggtgga gaagacggca gggtagctac ctgtgcttag tgtactcttt gatagttttc 34200 gtaagcgatt atgcggtttt ttagaaacga accaataaca taaatgcaaa cgatgatgtt 34260 gttctgatgg cagcctaata agccaagcgt cagtcgggag tgagtcgtcc tgattatcaa 34320 acgaccatgg agtgtcctcg tccgtgtatt agaaacgggg agttaatcaa tggtgtgtga 34380 tagctcaaat aggtagagcg tgaggctaca gtgctgaatg gtttgtgggt tcgattccca 34440 cccatgccta cagtccagac gatatctgag tgactataaa aacagatgga gccaggtgga 34500 atccctggca attaattccg gtgtaggtac tagtgtcgtg cacggcataa gtgttctggt 34560 tcgagtccag acgccggaac caattatcag ttgcacgaga tggcttgata tgttcaagtt 34620 tggactaagt gtgacaccgc tagattggag agctgctgcg gtaagctata gactccaacc 34680 gggggttcga atccctcact gataaccaat tcaaagtagc attgcactaa tccatcccgg 34740 agttcaaagg ccaaacatgg agcagtgcta ctttgaatta gaaagattat gggggtaagt 34800 cgtaaccata cgacggcgta agtaccagat gagacttctg gacgccccta ccagatttgg 34860 ggtgtaacag ttaaatccag ttgccggaag tgctattcta gatacgctgc caatgtgtga 34920 acggtaaggc tgtacaggac tcggactccc gcctcaaacc aaagtcgcca ggtacttatg 34980 gcaaacgggt agtaacgaac tagtcattcg tcaaacgccc accctattat gagtcagcaa 35040 gaacgccttc acttaaactt aaacaatata agcttaacgc aataattatg taggggtatg 35100 taaggattgc gaagaaggag ccagttaaag tctggcatga ctcactcatt tatgcccact 35160 tagcttagac ggggaaagca accgactaat aatcggaagg tcactggttc aaatccagta 35220 gtgggtactt attaatagag aacatagtcc tgattgcatt gcactctaaa gacaatgccg 35280 gtgagacagt ccggcactct aattattgaa aacataccta acggtgtgag tatgagcaga 35340 ctataatgct caattacagt taactattat cggactatca atcctgtttg ctggtacaag 35400 caccggtggt aatagttaac tggcctagcc tcgttagctg cgttaaagct actaagtccc 35460 tcttcggagg gactttttta tttgagtatt gatgaacgca atcatgctta agtacaaatt 35520 caaaccactc tatgaggcta ataatggcaa cagtaagtaa agaagcaatc gaagctaaga 35580 ttaagagcgt ttactatttt aatggtgctg atgcagtgaa atctgcattc gttgatcctt 35640 ctgcactccc ggcagatgat ttggctaacc tgggtctggt aacctattgt gttatcattc 35700 tggaaaacaa ttttaaagtt gaaggtgtat ctgcttgcgt agacccaact atctatgatg 35760 agcagttagg tcgccagtat gcatatgaaa atgcattcaa taagatttgg gaattagagg 35820 gctacctact acgtcaggca ctgcatgaga aggaagagac tgctaaagct ttggcttcct 35880 ttgcagaaaa caatacctgt gatggtggtg gttgtacaat ttgattcaaa ggtgtatggt 35940 agtttttacc gaaaggtact gaactacccg acaaagtagt aaggctcatg aagccaaaac 36000 aaaacaagcc ctcctagtga gggctttctc atttgaggta gttatgtcag aagaaattaa 36060 agtccatttc accaactaca ttggaaccaa gtgtgtaaat ggttttccga tgagcaaaga 36120 aacatactgc aaacttcgtg gttgggatgt accagcagat gaagacccgc tagaagaagg 36180 ctacttagtc gagtatccag actctaagtc caaccatccc caattccgtg gctatattag 36240 ctggtcacct aaagctgcat tcgaagctgc ataccgtgac gtagaaaaag gttgtacctt 36300 tggtcatgca gtagagcttc tcaaatcagg ctttagaatg acccgtaaag gttggaacgg 36360 aaaaggaatg tatatcacct tagtatctgg tgaaaattgg gccatggata aacatgaaaa 36420 taccgtttgt gagaaacggg attggctggg tattaaaacc gttgataacc agtttatgcc 36480 atgggttcca tctcaatcag atgtattagc agaagattgg gtgttagctc agtaaaaata 36540 aaagcccctc attagagggg ctttttcatt tagttagtaa ggttgagcca agggttcagg 36600 ttgtgggcac gtaagccttg accgaaaccg aaggagtaac cgagatttcc ctgtgcagca 36660 atactaaaga tgttgtcctg aataggcaga ccaacgttac caaacatggt aggggttgga 36720 gccaacatag ccatagcagc atggactggg ttattacgaa tcatggacac tgcaactttt 36780 gcagaacgaa tcttaaagtt atagaaccac atcagaccaa cactttccat atacccacgg 36840 aatcgaccag gcagacggtc atagttaatg aactcttccg ttacacgccc cagtgcttct 36900 tcacgagtct tacctttacg ctgagtcagt tcatcataga tgattgcttt agcaataaag 36960 tcagagtact caaccgtctt ctgaataccc tggaagagag cagtatcctt agtgataagt 37020 gcatagcgac ctgcgttacg aacagactta ggcagcttat cagccagctt ttccatgtat 37080 tcgtggagtt taccttcagt aatcaggatg tcatcacgac caataccagc atctgcaatt 37140 gaagagaact caccagcttc caacagaggc cagatactca aacgtttatg gctatcagag 37200 atggactgaa tctcagcctt gagtttacga atctggttcg ggttagtagc tgcacgtaat 37260 tctgcttctg catctacctg acgcagacga gatttcaggt actggttaat ctcagcagtc 37320 ttctgtgaaa tgctcttagc aatgttctta accggtacac cacgagcaac catctgataa 37380 aggttagcca ggaagttaac agcaggtacg actactgact taaccacaat cagagtctta 37440 gcttctttaa ctaagttctg aaccaggttc tcaccaccca ttacatactt ataggcacgg 37500 ttgccaaaga cacccagcat agctttcttg aaggtatcca gtgtttctgg tgaccaacgg 37560 gaattaccag accaggcatc acctacagaa gctgcacgat aacccagagc atcgttgagc 37620 atgtcacgac gtacccataa ttcacccgga ccaaacaaac tttctgcttt ctgacgggtt 37680 tcactgttca tcagcttaag tgcatcggca gttactgggt ccagtttaga accaagaagg 37740 ttaacgtact gggacttatt agaagcagac atcttaatgt cgttctcata catgctatgc 37800 aggttttcaa tcagcatatc gttgaatcgc tgagccttag cttcttccac ctgacgacca 37860 cgccatacac cgattgcacg agcaagatgg ttctcacctt caatgtgctt cagcatgtta 37920 gggtcaatgg attgctcata agcaaccaca ttaccattgg catcatatac aggcagcagt 37980 ggttcattac cacgttcacc acgagccagt gctttagtga tacggtctac ggaaggcttg 38040 tcagtgatac gaccagctac catggttccc atagtaaagc ccgtaccgag atctacacca 38100 ccagcagtat tacgaacgtt ctgtaagata ccttgcgaga aaggagcctg tgcctgtact 38160 ggtgcaaagt agtagctacg tgctggtcca cgattagcag agctaccctg ataagtacca 38220 agacgtacat aggatttctc aatcaaatca gcaaactgac tatcttcagc aacaatcagg 38280 ttaacaccct gcttgttctc gctagggata taccctttgt actggttcaa ggttgcacga 38340 ctatcagact tagctttagc catttcatct ttacgctgac caaccagata agaagtagca 38400 aagtccatgc cttcaatttc tgtctgagcc agtgaagata acatctcacg gtcagtctta 38460 ttcattgctt ccagtgcata caaagtaatc agtttatcca actgagctac atctacaaca 38520 gaacgtgtag tcttacgttc acccagtaaa cgagaaattg cagtagcatt acgtagcagg 38580 ttgttaccaa ccgtaccttt aatcatgtac tgagccagtt gcttagattt acgattaatc 38640 agaggccagt ttcgtccagc ttgtttctgc aaatctgctt ccagtttatt aacctcacgg 38700 tcaacaatct tttggtcagt cagcaagtca cggatttcat ccagagacat agtgtcacgc 38760 agaacagcta agtcagtttt acccatacca gtatgcattg ctttccactc ttcattagtc 38820 agcttacggc tgaacttaga tgcgatagtg gtaggcaagt gttcacggaa ctgttgacgg 38880 tcagcctgta cctgtgcacg tactgcctta atcaaatcat atacagaagc attgctctta 38940 gtacgtccga ttatgtcatt aaccaggtca tggaaaggtt gccatacttt accctggttc 39000 attgcagcca taacaccttc ggctacgatt gcaccattct tctctgtagc aatagcagct 39060 accagttgtg cagcatgaac agcacctttg accaatggat tcttggtatt agctgcaatt 39120 tcacgagcac cttctaaagc acgggtagac aatgcatcaa tagagtccac caaatactgg 39180 ttagcacggt cgatagcatt accggtagga gtagcaacgg aatcatagaa ggattctgcg 39240 ttaagactgg tctgcatgat tgtctgagct aatgcatcca taccttcctg tacgttagtg 39300 gctttagtat cacctgctac acgagcgttc agactagcca ttgcagcagt accgatgtta 39360 gtcagcattg catcaacagt attgccggat ttcttatctg ctttcatgac aggaatatca 39420 gccagtacct tacgtacctc ttcacttacc attgccagac caacaaaggt aggtaataag 39480 gaagagcgac cttgagcatc aaactcaatg ttattagcac ccatgatggt atcgaacttc 39540 tgctgtgcat aataacggtc agcagggtta gtactatccg ggtcagccat aaaagcttca 39600 acagtcaggt tcttagtaac atgtgtgtag tattcctgtg cacgagccat agcagctggg 39660 ttaatagcag cctcagtagc cagtgcagca acaatgttag taaagaggcg ttgttcctgc 39720 atattcatgg tgaagccatg agcctgaaca tcacgagtta ctttagttgc attcactact 39780 gcatcagaaa acttaccttt tcgaattact tgctgtactg gttctgaacc gatgtagtca 39840 gtaatcagct tatcaaaagt cttacctaac tcttccagac gagtattgtc accatatgct 39900 ttgttatgga acagggtagt gtctttggcc acagcagcag taggagcttg tccacgcatt 39960 actacagcag agttaaacag taggccagag aacatatcat ctgcattagc cggagctttc 40020 ttacgtccaa ataccagacg cttgattgct tcatatacag cctgaaccat agctttaagt 40080 gcagtggttt tcttctgctt accaatcaat tcacggttgg ttaagcccca tgccatgtac 40140 tcattcaatg cagcagcttt agccattgct ggttcaatga agccattaga caaatgacca 40200 ttaatggtat tgagagcatc agcatatgct tcacgtactg ccgggggttc attcttaaca 40260 tccagagtac ggaactggtt catcaggtct tcaatgttct ggactgcttc attaggagtg 40320 ccttcatagt gagccagtac agattcaaaa gtagaagcat gaaccaattc atgaaccaga 40380 gtctctaaag aaggagtaac taaatagatg gtcttatcat caaagttagt ccagccgtat 40440 gcattacctg cttcagcagc ttcaatatct tccggtgctg ggcgagtaat attcttctca 40500 atagcgtagg catccagttg agaaggcgta ccataaacaa ccttgtagtc tttagcagca 40560 agggatttct gtacttcttt caatactgct tgctgttctg gagacatctc tttagccagc 40620 ttagtgatag cagtattgga caacagacgt acaccagact tcagtacacg accaacctgt 40680 tccattgccg gtacttcttt agctggttct gctttagcag cacgtactgc atcacgacgc 40740 ttatttaatt cagcatcaaa cagttcgttc agctttgcta cttgctggtc tacagtcaga 40800 ccttcaagag aaattttacc gtcgttcaca tatggagcac caacagcagc catctggtca 40860 acagtgactt gtacctggtt cattaccttg tggcgaatat ctacacccag agcaatgtta 40920 cgcaggttac gttcaatctg ttcagcacca acacgtaaca ggtcatcagt agcaccttca 40980 cgctggtcat attccagagc agacttagcg atagcctgtt tggttttatc agacagtttg 41040 ctgaagtcta cattcttcat gaacttagcg taggagtcat aaacattctt gatagggtta 41100 ccctgccagg aagtatatac agcctcatta gctttacgac ttgcatcagt aatgtcattg 41160 ataccaatgt tcataccatc aaagattttc agggtattct tcggagcacc tttcatagta 41220 gaaagggtct gcatcatcat gccatcacca gtaccgatgg tcataaatgg aatacctgct 41280 acacctgcct gtgaaggagc atagatgctc attggcacac gcatacggtc atctaagttg 41340 gtagccaata cttggttagc aacatcagta ttctcactac cagcaatgta gaaagtctgg 41400 gaaccagtct caatcattgg agccaatgga gataatgatt cctggatttc attcaattct 41460 ttctgtgtta aaaaatcacc cttcttccat gttgggtctt tttctttttc tgccagtttc 41520 tcctgaacac gttgctggaa catgtcttgc agaaccagag attgaatctg ggtagctttc 41580 tgcaagttct cagtagagta catcagacct tcacccacgg tatttcggat accagtacgc 41640 attggttcaa cgaacagatg gagcatattc tcctgcaagt tcttcagagc attacctgat 41700 acagtaaact tctgagggtc taacttacca gtcagagcac catctacctg ctgaatagac 41760 aactcacctt tacgcataac cggaacatta ccagttagtg cttccataga agtaaggaaa 41820 gtatccagca tagcctgagc atcagcctca gacgcagcct ccttaccaaa catagccata 41880 gctggggaaa tgctagggtc agcagcacgg gcttgtagaa cgtcactgaa tcgttcatag 41940 atgacatcag taattgcact aaccattttg cctgcaatac cacgagcacc agaaccatag 42000 atggtaatag tcagtgggtt tttagcgata ccacgtttca ggtccagagt accatcttca 42060 ttcaaattaa agtctttaat gaacaggtcc attaacttct ggagatggtt catctggttc 42120 ataacaggaa cgttgttacg gtaagtacta cgtaattcat tcagtgcttg ttgcagacca 42180 ttagtggatg cttcatacag gtcaacgctg tcctcatgcg aatgatgctc gttcatggtc 42240 ttaccagctt taccgaagaa caaaccacct ttggcagtat tcttaatcca gtctggggtg 42300 aacttaccac cagtcattaa taccattgcg ttgattggtc cgttggttac accatcagct 42360 tcaacgtaca gtggggtatt aaagttggta cggtcatcac tgttaaggta acgggcatat 42420 tccatcagag ccataagagc tacgaatgat ttatcactac ccaaagaagt cttcagaata 42480 tccactgcat cggcaggtaa gtgtccagac ttatcaaatt caaccatcat atcaacggca 42540 ggtttaagtt tgccttccag agctttagtc agctcatctg acataacttc acgggacatt 42600 ttatgaactt tgatacccaa tgcctgagcc agtccaagct ggaagtcaga gaacgtttga 42660 ctgttttcat tacttaagtc gatggtagag aaggttggaa ggattgcttc acgaaccagc 42720 ttactggatt gtgggttgtt cttaccaagc atctgcatac gacctacacg ggtcatgttg 42780 tagccgtagt gaataggtgt atccagtcca ttctcctgtt ccttaatttg attgattaca 42840 ccaaacaagg aatcatacgc catggacacg gacaggtttt taccttccag agacttagca 42900 gtattcacat tcagcaattc tggattaaga gcaccagcac ccattaactc cagaatgttg 42960 tcacaaccca atgcttcata gaaattaacc atcggcatat gtacacgaaa ctcagtagct 43020 tgttctgctt tgagagcagc tttctgttcc ggggtattct taacagccgg gttacgtaac 43080 tgagtttgag ccacggaagg aatgtcatca cccagataca atttctctac tggttctaaa 43140 aggacagttt cctcaatagc agtagggaat ttattaatgg catcgttatc atccagcttc 43200 tcaatggtat acagaccaac agtcttatta gattctgggt caatctcaga cacatctaac 43260 atggactctt taacttcacc catctcaata agggaagaca gaatctctgt agccattgcc 43320 attgggatac ccttggtata ccccagaggt gcattaggat tacgattcaa accccaatag 43380 gattcaatct tttgagccag tgagttagta gcttcaacca gggtctgagc attctcgaag 43440 tcagccagaa taccttctgg tagcagagat gcttccacac cagtaatagc agctacgtct 43500 ttcatgtctt taacagcagc attctgtgta gcagtcaatc gccattgcag accagcaagt 43560 acagcagttt ctaatagctg gtcattgaac ttaaaggtgt caccatcttt ctctacgatg 43620 ttaagtaact taccacctac ccaacggtta gcctcagtac cttccgcaaa acgtttacca 43680 acgttcttgt tagcaaggaa cttagccaga cggtcagaca gagtagtctt cagagtttca 43740 ccaaactcaa ataagtcctg ataacgctta atcacatcac ttgtgagagc gttattttct 43800 ttttgagtga atgcttcaaa gcgagcagca gaagacagtg ctttcttaac atcagtcaaa 43860 ggagattcag aaccaatggt acgagactta ggctcttctg gtagggaaaa tgctttaagg 43920 aactggtttg gtgtcttatc attattatag acagggaaca ctgtttccat ttccgataaa 43980 ggtttaccac caacggtata gtctgctttc agttgagcca gtgatacttc ttccttctca 44040 acgttattgg tattacgttc agacgtatct acaccaatct gaataagtgc attgtttgct 44100 tcaacgaaag attgaacagt attgaattgt tcagcagtca gtttctgatt atcaaacagt 44160 tgcagagata caaatggttg ctcactacct ttatagaagg tagagcgaat agcaccagta 44220 tccagagaca tatcacgata accatcatgc ccaaatacgt cggacaagct gatatggtcg 44280 ccattagaac caaccacagt accatcagca aacaaccaag ggctttctct acggtcacca 44340 ttcttattaa gagtttcctg tgaaatagta ttttctttca cataagcatc tctttcagat 44400 tcagtagtag agacttcttc tgcttttgga ctagtctctt tagtaggagc agtcttattt 44460 tctaccactg gtttatcttt aggctgtaca gtagtggtgt cctcttgcac tagtgcagga 44520 gtttcatcaa cctgattcac tggttcatta gtctgttgag actgagtggc atcaagacca 44580 ccttgacgga actctttaac tacctgggca gctggcttgt tcagacgtga atccaacgag 44640 gtaactttca catgagaaac attaagctcc gggtaagccg tagcaagtgc gttagcaatg 44700 tcagctacgg tcttagcttc cagtgctact tgctgggcaa acttaacaga cttggtatcg 44760 taaggattga caccgaggcc agtacgacta cgtacccatt cacgggaagg cgagagagcc 44820 tgataatgaa cagacttatt cttatccgca ttccccgaaa tcagatgctc attcaacgct 44880 ccgaccttat tctgcatgtg ctgggcgaac ttcataaaat cgctcagata agcggaggcc 44940 aaatcgaagt taccagagtt atacgcagaa cgaatgcgtt tcgcatgttg caacgcagag 45000 tactgtcctt cattagaacg agactcatcg gttttaattt gtttactaac aatgtcttga 45060 ggacgtaagc ctagttcttc tgctttagcg tcgaactctc gtgcaccctg taataaggca 45120 gcagcagatt gcagggcagc acgttgacga ttccccagtg taatcttacc ttcacttgca 45180 tgtttcagaa ccatgttcac tgaatctgca tccagcactt ctggagatac atcagcagcc 45240 atagcaatgt tgtttgcttg ggcttgatta gcttcttcag tagctttcag cttacccgct 45300 tcagcctgtt cctgaatcat ggaatggatg gcacgaaatg cacggagtac tttaggggta 45360 ttctgtacgt tagccatcag gccggagaat tggtctacga ttgcagcagc aggggaatct 45420 tgttccagat tagccaaagc acccgggtca cggttaatga agctatccat agacatgata 45480 ttgtcataca tctggattgc agcttccatt tgaacgttag ggtcttcggc agtattaacc 45540 aagtcagcca tcttctgaat agcttctaca cggttagtgg aaccagaaac agcttcacgg 45600 attgctgggt tagtttgttc caattctacc gggtcaaact tcattgcttg agttaagtca 45660 gcagcatact gggtagcagc agctttctct tctggagaaa tatccattgc atccactgct 45720 tcctgaacag ttgcttgagc ttgttctgcc tgagcagtag cttcttgtgc agcagcgtta 45780 accgtagcat cagatacggg ggatgcttgt tcattacgct tagcaacttc ttccccacgt 45840 tgaaccagga tgttagtaat aggagaagct acttttgcta gtgccttacc agccaaagaa 45900 gcaccagcca gggtagtacg tacagcagga ccaacggcag caccagcagc cttaacagtt 45960 gcaccaggag cttgagcaac tccagcagaa ccgaagccat acaatgcacc aagaccggtt 46020 tgttcaccca cacctttaag caaatcacgc ttagcatcta cattaccttg aattgccttg 46080 ttctgtgcaa actgacttgt accagactgg ataccttctt ctacagtctc acgcagcatg 46140 ttagaaccag caccagccag tgaacctacc ttaagtggat taagttcaaa cttggatacc 46200 agtggaccag taagagcagc aaccggagca gttaagccag cagcagtaat acccgtttca 46260 gatgcagtct ggcgacgtgc ttcttcagga gataaaccgt ctttaatatg ctgttggtat 46320 acaggagatt tagcagccaa ttcactgaat ggcatttcca taatttcatt agcagtctgt 46380 tggtatgcac caccagcttc cataccacca attgctatag caggtgcagc aacacgacca 46440 gtagctaatg cagtacgtgc aggacgtgaa cctaactcag cagctaaggt aatgccttta 46500 acagctttat caccacctac cattacttta ccgagtgcag atacaccacg aatcaatgga 46560 ccgccggtaa agagagaacc aacaccctca gccagaccat cagtagcagc catgccattg 46620 gacaaggtgt tagcaacaga atcgtaagca tctcgaccaa tgcgagacag ggaagcaacg 46680 agatcacttt ctcctttagc aatatcctgc ttatagagtt tctcgttttc ctgtgcagat 46740 attacgttct ggttctgtac tacattacga cgagcattta atgcatcaga ctgtaggttg 46800 tgtacacctt tattcaacca gtcaagaccg gaagcaatgg ttgccccggc attatcattg 46860 accaagcctg taccaagagc agcaatgccc ccaagagtat tagcaacacc aagaccaaca 46920 ccagaaaggg tatcaccaag tgcttcacca tatgtacgac tcttagttaa atcacgacga 46980 acagcattag ctgcattgac acgagcattg agaatgtcca ttccttgctc attgccatac 47040 ttgtttatga tttcaagagg agaggcatta gtaaaatctg cctgaaaaga acctgggtca 47100 aatgcaccag cacctaagtt accagcacgg cctgcctgta attggtaagc gttctgtggg 47160 gttagttgga aggggctttg cccttgttca gcttgtttcc gagaggttgc agtagagaca 47220 tcgacttgct tagcagtagt gatgctgtct gcgaaacctg ccagacggtc aaatgttgac 47280 atagcatgat tcctgttcaa acttatatcg tataagaaga ttcactgtac agtaagtgaa 47340 tgtaagagaa aagccccgga tggggcttta ttagtatgta agattttatc gaggttgcat 47400 atagggaggt aaaccctctg ctaattttcg ataacgttta gctcgttctt catcttcacg 47460 actacgttca cgttctactg aagcattaga acggaagttc tgtggaacca gatttccctg 47520 gtcagctacc acagtattaa cagcagcatc taatgcagct cgtgcccttg ctaactcagc 47580 ctcacgagca gggatagtag cagcaagccc gctttgccca gcagctacac gacgacgagt 47640 atcagcaagt aaggcttctg cttgtgttac ctggttctga gcattagcaa tattaccctg 47700 tacctgggta agaacattat cacgagcagt agcttcaatg ccttccccac gacgtactga 47760 acgtgctagt tctctggcaa cggtatcatc aatacgaata cctccaccac cttcattgga 47820 gatgaacgga ttaagtgcat caagtgctcg actaatcata ccttccggta cgttggtagt 47880 agactgctta agaatctcag cagctacagc aggagactgc ccggattctt gcataacatc 47940 attgagtcga gcaatcatcc atcctttttc agaaccttta aaagcatcct taagaagacg 48000 gtctgctact tcaccaatag tagagttgtc acgtaatgca cgagcataat tagcagtaat 48060 ggttccagtg ttatcttcag actgacgtgc agcaactgta ttgcctgcca tgtttgagac 48120 tacttgctga gtctgtaact gatcaagggg tgctcctact tctgcctgtg caattaactg 48180 gcgtgcttca ctccacggca tatccttata accacctaca cctaagtcag gcaatgcagc 48240 ccaggtctta gaaaggttac cattcttacg gtcattgaag attgcttctg ctattttgtc 48300 ctgtacttca ggagtcattt gctgattacg ccaatctgaa ccaagcactt taggggcata 48360 gtcttccaga gtagctttat taatctgaaa tgcacctaca ggagaatgac cttgtgacgg 48420 gttattaatc atactctgtt gatgattagt aacctcacct agtgtcatct cagtaatagg 48480 cttatcagta gcagcaaagg tataggttgc atcataagga gaaccagaac gagtaccagc 48540 agttcccgga gcagagccag aaggagacgt agcgtaagta cctgggaatc tctggtttac 48600 acctcgcatg attgcagcac gtacacctgg agaagcattc tgcatagcag actctgccaa 48660 tgtaagagca tccataggag tggaagcact acggaaaata tccgtcagta taccggatgc 48720 agcttgtgaa tcagcatcat tacgttgagc cacacccaaa tcaaagcggt tctgtgcttg 48780 gttaatagcc tgaccacctt gcccttgaag acggctcagc atatccagtt gttggtcggc 48840 agggagtgca gataatgcat cacgggtttg gtccaatgca gtattaatac ggttctggtc 48900 accagactga taagcctcag acaataaacg aatagctgga gaagcattat ctaatgcaga 48960 gtcagtattc tgtaaacgac caaaacgata agcattgtag tcattaattc cctgctgacc 49020 ttgttgagtc agaagagtac ttgctcgctc atccagattc tgcagggtat gttggtttac 49080 gagtgaaggg tcaaccccct ggaacaatgc accggatgcc agagcattac gatactcagt 49140 agggtcttga tactgcatag cattcatcat gacggcattg ccagcctcct gcttggcagc 49200 gttctggaag ttacccagtg catcacttaa gccggaggtg gcgttaccaa tcatgttgcc 49260 aaatgtgcga atgctgtcac caactccaga gaagttaggt gcatcaacat tacgccatgt 49320 aatttgagcc atgatggttt cctattaacg agttagctta ttagctgcaa tgtaagcatc 49380 agctgaagac tggtcacggt tctctgcaac agcacgacta cgagcacggt cttccagtgc 49440 agtgttataa gacttaatct ggttgttcag gttagtgtta gtaacactct tagcaaagtt 49500 caactggtct ttggctaact tattggcctg gaaaccacta tagataccag ctaaggaacc 49560 aagtgcaccg agtcctagtt ggaaggtggg tacattcata cccaactgat tagctgaacc 49620 agacaagaag gaagtagggg aggctactcc tgtacctgca ttcataccaa tagctgcacc 49680 cggattgtag ttcatagctg gggtattaaa attctgattg ttattagaca tccaagacat 49740 agcagctggc tgtggggttt ggttgcctgt taagaatgac aaatccatag ggattctcct 49800 gttaaacaag gtcagtatta agagtcatat cagagtagct tccaatcatg tttaaagaca 49860 tgtcagctat atccgaacca gtcatgagag tacgtgagag gaaagaatcc atcgactcca 49920 ttgacacaaa ttgcataggg tcaattacac cctgcccagc agtaccaaac atctcttcat 49980 actgcttatt gattgccatc atatcagtat tgtactgctg cattactttc tctgctttct 50040 gaatagtagc agccgtagat gcattaatat actggctaat accattacct actgaactgg 50100 taagctgcat gatgttctga gcattcatca tttcactggc taaggtagat aaggatgaac 50160 cagtagatag agcagtacca acattcatag ctaccattga agcaacagca gtaatgataa 50220 aacctagctt atcaccaaag agtgaagtgg atactttagt gataatagat accagaatca 50280 ttgcagcaat ggcgtttgct acagcaccta ctattacagc agctaatcca acaaaaccga 50340 gtgacgcccc aactgctcca tatgccccaa gtatacctgc accaccagta cccatagtga 50400 atacggatac aacgacagca accacaacaa ccacaatctt aaaggcagat gtttgatacc 50460 acttctgctt aaccttctta tatgagttca tcactaagta ggagcaggca gtggatagct 50520 gagtacttcg aatcagtgac attgaacgat agatgttagt atgtagtgga ataatgaacc 50580 cactctcctc tgcatcaccc attgcttcgg caacatcaat atgcactgac ttattcttat 50640 ataccctgtt attgtgattt agacccagta ctctaagttt acggtaagtg ttattaccat 50700 cctgccaaag cagttcatac tcctgcatag aatagaatgt agtggtaacc tctaacttct 50760 tagctgaacc ggagttacta gcagttcgta tgttcttgcg agtaagagtt atatcccctg 50820 catacctggc tcgaagttgt ccttgcttag caccagacca tgcttgccct gagtgggtag 50880 tttcagatac gtaattccaa ccaatggtca tgtcatactt gtaatgctta ttactatgta 50940 ccctaaattc tctcttaggt atgactgggt attctggcag tggtggagga gttccaatag 51000 tgtgcccttc agtcctattc caccattcta catatgcatc aactgcatca ttagctgcct 51060 gataacctgc aatcactgcc tccaaggttg gataggttgg gtcaggtggg aatgcttcag 51120 tagccatctg aaagaaacgg tagatgtatt ccttagcagt atcttcagga gtattaagag 51180 atacgccaaa tgtgccataa atgtactgaa tatcacctat atcatcattc ttcttaagct 51240 ctgttactac cttatcaatt tttccaccgg tagctttata aagagctttc ttacagtatg 51300 gatagattgg gtcattctct acccattgtt tatcgttcct gattggaata aatggataga 51360 atcgattatc ggtagcctca gtatcgaaca gtgagtctag tgcaacgtta ccagagttct 51420 gcttatagat aagcatctta ggtgtaccaa cagcagtcat ctctgtatct gtacgagtag 51480 tctgggagga atacttacga accagtgtct ctgtggttgt agtaattgta tcagtacgag 51540 taacaccacc acctatatca actacgttgg tagttacagt agtgctggat tgaatctcac 51600 caaacttttt atgagtcatt accctgtcag atacaactac agttacatca tcgggtttct 51660 cagtgaatcc tttacggtaa accttaacgt aagagttcca gttatccaca cttggttcag 51720 tgacagtatt gtcttcatct ggtctaccat cagagtaaac agagtgagta tgtatggtct 51780 tagtaaggtt agttgtatta gctgttgtat cacttgatat ggtagtccat agcaaagtgg 51840 aaggtaagtc accctcacta tcatagacag tagtcggtcc tactaccggt ggattggtag 51900 agggagattt atagaaagag taatcagcat agagatacaa agcacctggt tcaaagttag 51960 taggtgtgaa tttaatagta gaaccaccat ccagggaggt catagtaatt tcattagtat 52020 cttcattgat gtctatgtcg aatcgttcca tgattcgact aggagcattt tcataaagat 52080 attgggcaca ccattgctca aagtcagcaa agccaatttc tgctgcctga acatagacag 52140 actctccagc aggtggtgta atttgaccct ctataacagt agggtcaatc ttagccaata 52200 cacccaacga agaaccagcc ataccaactt ctgaatcata gtggttctta ctccagctgg 52260 agaacaatcg catacgaata ccaggtccat tcagataact atcagagata gtgtctgcca 52320 tagtgaatcc tgtattggaa acaatgttac cgatgactac agtcttcata tagttaggac 52380 gtttgtgtat atcccctgcc atgttataga cagaggatgc tacgtatact ttagtcttcc 52440 cactgaataa gcccatatta gttcagcccg ttgttagtct tcagcttggt caaaatggta 52500 tcaatacttg cgttagtgaa accattagga ggattcaagc cttcatcaat agtcttctgt 52560 gtaatccatg catcagtaaa caacttagat gctttgactt ctgcatcacg ttggtaagaa 52620 gtaatctgtt gagagtacaa ctctttctgt ttacctacag aaccagtaac agtagcacca 52680 tcactacgag tatccagtgt ctgtgcacgt tgtgcttctg tctgctcagt aagcaattta 52740 agctgttgag gtaacatctg attagcatta aacaatgcag cacaatacgt ctcagactca 52800 gtagcaatct tcatcttagt aagagcgtat tcactcttag cagacaatgc ctgaatctta 52860 gccagtacaa actgagcctt agaagtagcc agttgaacac gagcagtaac tgcctgaatc 52920 tgtgccatag cagcagccca gtatgcctgg tcacgtccaa gtaagaactg aacagcatta 52980 ctcatgcatg attccatcat agcaatgtat gctttggtat attcaccacc agtaatacgg 53040 ttagctttaa actcagcttt aaggtgattg tgagcagatt ccattaatgc atcaaacgta 53100 ccactgcctc ctacttcacg ggtagtaaga gattcattgg ttattttagt aatagcccca 53160 aagattggag aatcatctcc accagggata tcccattcag gaccagacat atcaatatca 53220 ggaagggtaa agtcatcacc cttagttaac tcttctagga gtcggttagc ttctacctca 53280 gcagaacaag acataatcat tcctcttggt tcaaaaagaa acggcccacg gagttacccc 53340 agtgagccgt gttgaactgt agcttatacg ttaatcgtta aggctaccag cagcaatctg 53400 tgcttgagcc agctgagcca attcagcttc agtcaacgga ggcagtactt caatggagaa 53460 ctcacgtgct tctgttgcac gaatgtccgg taagccattc ttacctttac gagtagtaat 53520 gttaatgaac ttacgttctt taaggaactc gtagatacag tacggaatat gataaccatt 53580 gtcggttact tcaccgaacg gaacaaactt acgtacagta cccatatatt cgttagctac 53640 ggtgataatt tcacccggca ggtctttctt cttagggtca aggttctgga tacgtacacg 53700 aatcagtcga gtctgttctg cacgaatctt ctgacccagg gtcatcttct taacaccagc 53760 ttcctgctta gcacccaatg ggttaacagc agcttcttct actactggtt catcttttac 53820 ttgtgcagct tcaatcttct cacgaagctt ttcaacggaa atgttgttag agaacttaat 53880 attcatcaac gttgcacgtt gcttaagaac ttcaagttcg ctaggcattg caatatcgtt 53940 aacggtatct tcgttgccct gtacgttcac ttctacgtca gtggtcggtt tatcgttaat 54000 gctcatgttc atatttcctg tggttcattt agttttatta agagggggac ttatgtcccc 54060 cttgttttat ttcggactat tacagaggag caacagtctt aatcagagcc agacgttctg 54120 gacgtttaac caggatacca tagtaccact tgatagaact gaagccagtt tcgccatacg 54180 ggtcattacg gtcagcagtt tctttacccg gcatcttagt catgatggtg aacttaacag 54240 acttaccatc agtctggaag ccaatggtag agaaggagtc atcaccaact accagcatcg 54300 ggaatacgtc gtagtgttcc tgaccagata ccatagaggt acggtaaccc gggttagtag 54360 taacctgagc accagcacct gcccaatgca gcatctctgg aacctggatg atacggaact 54420 tatcaataca accaatttca ccattcatca gagtaccagc atcagcatag tgctgaactt 54480 cgatgaatgc tttattacca aacaggtctt tcatcgcttt cagttctgga accagttcag 54540 aaccaacgta cattacacga gtaccaccga gtactttggt atctgtcagt ttagaaccag 54600 tgatgatagt agtctgggtc ggggtacggt tctcagtaag aatctggtca agacgcatca 54660 ggttcttata agaaactaca gacggggtag aaccttcacc agtaatggta gcatcagaga 54720 cggcagcacc tgcatacagt acagtaccag cagcagccag caggtctttc tggagaacag 54780 cttcagtcaa ctgtacagca ccgttcatca gttcacgaga caggtgttct ttcagttggt 54840 catcagaatc aaagtccagg gactcttgag taaattcgta gaagaagccg aatttatgaa 54900 tagagccttc acgagccaga cgagtaaagc ctacacggtt aacacgacca ccattctctg 54960 acagcagagg cagtttagaa gtgatgttac caacgtcttt ggaagagcca taaaggttac 55020 cgttaacgat ggtagcacca ttagcatcaa taccctggtc gttaatgttc ttatcgtcga 55080 gcaaaggaac atactcgtac accttaacag tcttaccgta gttcttcggc atgttaacgg 55140 tgttagccag aggcataaaa tactggtctt tacgagactg aataatagcc ttcttcagcc 55200 agtaataggt attcatctgg tcggaaccag caccatcaat gctagatttc tgaccgtcaa 55260 ttggagcgtt atagtttaac atatcatctc attcctgttt aaagactacc cggtactggg 55320 agtttagcga aatcttcatc actcatagcg agtgggttta caataggcgt tgctttacgt 55380 gtagcagccc gattaaggga agctgcttta gcttgctcac tgttagccag agtctgctta 55440 ggttgtgcta cacgcactac cggctgaact gctggtttaa ctgcttgtac tggttcaggc 55500 ttagacactt ggttaaatgc tccctgctgt gcaagaaggt taccaacgta gttataagcc 55560 tgaataaacg gagtacctac cggaatctga cctaacacct gaagacgatt cacttcatta 55620 gcaatggtgt cataaatacc attctcacgc tgttcgtgaa tggtgtggag taagccacga 55680 ttctgataaa gagcatcttt actggcagca tcccatgtcg agctaataac acctaatgta 55740 gcttgtcctt cttgagtaga ctttaagtcg tcaatttcgg ttgcaaaatc tgcttcggtg 55800 tctgtaacac ggtgtttgcc accctggtag ttaatttcct cttccggatt aaagtccaga 55860 ggatctgtac cagagtcttt caacaacttt ttaatggctt caggattctt cttatccagg 55920 tcaatcagga aagaaagttt ttcctcatcc attaaaccgt tgttctgtaa catcagcatt 55980 accttacggt acggctgaag ttcttgcatc ttacgagtat agttagcacc catctgcatc 56040 aggctaatgg cctcctccgg tgaacgggga gtaatcattt tgccgttagc tttaaaagga 56100 gccatcaact tctcataacc ttccttatag ttgaagtcag caggcagacc ttcagactgt 56160 ttgccttctt ctttctgttc ctggcctggt tcagcagtag aaggttcagc ttcagtaatc 56220 ggcttaccgt tactatcaac ttctgtgtca acaactttat catcaacttt atcagaagtt 56280 aaagaatttg ctgaatcatc ttcatctgcg tcagtttcgg gggaggtttc ttcggttggt 56340 aggtcttcaa cagcaggggt gtcaatttct tcatcaggag tctgtacgcc attggtttct 56400 gggttgttct gagtggaagt atcttcctca gcaataatag ctggggcttc catattcaga 56460 atctcatcat ccgacattgc gagaatgtcg gaagctgtgg ttgcagcttc cgtagtcata 56520 ggaaatatct ccggttaatt attcgtcttc gggttcagca cgaactgcat cgagttcttc 56580 ttctacctga agaataacgt cagcttcatt ttcacccata cgaatggcga ggtcgagcca 56640 acgacgtaag tgaccagcag cttgagccat gttaagtgca tctgcacggt tattcggttc 56700 aagcagtggg tcaccagact cctgcacgta acgtgcacaa tcttctacac agaactgtcg 56760 aaggattact ttacggaaca gtggattctc cagaagttta cgtacatcct ctgcgtgtgc 56820 aactgcacct ttagcagctt ccagtcgatg ttccagtcct gcgattgttg attctttact 56880 catgtgaagg tctgccttat aagttcatac caagagccga tgctgggtct tggctagggt 56940 cataaaattg ggaactaaga gaataagtcg ggtcttgctg tgcagctaag tcacgttcct 57000 gtaaagagtt cccgttagtc aaagcgttat atccgacagc agcagatatg ttgggggtag 57060 tttcaccttc tttagtaggt gttgtcaacg ccttagttat ttgaaggttc tggtttcctt 57120 gagattgtgc tttttgtttt tccatatcac gagcatgttt agtaccagat tcctgttcca 57180 gataatccag gtctttaagg tcaccactag aaatagcttc tttagcctta gcattattga 57240 gtgcaatctt acttttcaac tcttcattct caagctgtgc tttttgaatt gccaattgct 57300 taagctgttc ttccataggg tctggttgtg gtcgccaggt acgtaattca tgagcaaggt 57360 ctggcatacg cttaagttct gcaattttag ctacgagaga taatgtaata gtctggtcaa 57420 ctgtattacc taacgtttga accatgaagc tcaagtcctg agatttctgg ttatcaattt 57480 cagcagtatt aatatcaacc tcaatatcga aattaccttt aaggtcttca cggttaattt 57540 ctacgtactg ttcattggta atacgtacta cttccttctc agataagaat acggcattca 57600 ttgcacaaat cttagtacca atgtctgcca tacccttagc taatcgacga aggattgcca 57660 tctcacgttt ggatgctgca tcgagtgcac cacgaatacc agcagccaca tctccataag 57720 ctgcaccagt tacacctcca gagaatgctt taacaccagt tagtgcttct gcttcctggt 57780 tctgcatctg agtcataact attgccgact gaggtaactc agggaactta tgttccataa 57840 tggcctgact agggttgccc tgcattgggt tatactcata gtcttgtccg tcatcatatc 57900 gacgtcggtt cagagtatct aacatccctt taggataacc acgctgcccg tttgcacttc 57960 gacctaacag gtcaatcatc ccacgcatag ttgcaccaag aattgcttgg ttatctccta 58020 acaattctgc atctgcttca ccgtagagtt cacgtttacg tggcatgtat ggaacaacta 58080 ccaaaggtaa cttaccatca gggaatgggt tcttctccat acgaataaga gtagaaccaa 58140 tccaggtagc aacaatcggt tccagtgaac catcatcatt aatgtcgtaa aatccccagt 58200 attcataagc gactactttc ttacgtagtg catctttaaa ctggaagtca ccaggagttt 58260 tactttcatg gtctgggtca gtcataggac tggaactttc ccagtcaatc ttatccaaat 58320 tgtgataacg gtctttgttc ttcatgaggt ctgctttgca cgtctcaaac gagataactg 58380 catacagagc cttgtccaaa tcaccattac aactcgggtc aataactacg ttattaggat 58440 taagcatttc aacagtaggt ctgttaacca gtgccttctc tacctctacc tcagtaactc 58500 cagtctggat tgcataagta gcttctccag tttcattgaa gtagttaaca gcttccttaa 58560 tatcttccgg cattgtttcg tcatactcac ggggattttc tgcctgaagc tgtaaagcct 58620 gctgaagaat atctgcttgt tcctggttct caattggata caactggaag actggtgttt 58680 ctgttttaat cttaacggtc ttacgttccc aaccaatacg ggcaatacca gtaccatcat 58740 ctacgacact atgtacgtaa tcatccacca gttttacttt attaagctgg gtacggaatt 58800 ggtagttaag aactaattca ttctgtcgtg cagctaactc atcctcaaaa gtaacagggg 58860 ttaccttaaa gagtttatta gatgagagaa atggttcaga taatggtgca taacgccact 58920 ctgcctgacg gcgaaccagt ctaggttgga cttgtgagcg tcctttaacc tttgggggtt 58980 tagccttgcc tttgacttcc atcaagtcat tccactcacg aatctgagcc atgattgcat 59040 cgtgagcagg tttagctgat tccaaatcac ccttcagtaa ttggatactt ggttccttct 59100 tccagtccgt taacttctct gattgagccg ggtctggtaa aggcttaaaa gtgtcttggt 59160 gttccatagt tattcctgtt caaaaagttt acggtcagcc tgaatctgtt tacccaactc 59220 aattagttgg ctgtcacgga gtctaacagt tgcccggagt tcttcaacca aacgtctgcc 59280 ttcttcaaga ctgttgtcga gtctggctgc atggcttgca agacttctgc actcaaaggt 59340 tccggcttcg gcttgacgtt tatataccga tgctcgtctt tcagactgtt gcatccggct 59400 gtcataatca ctgctaacgc gagcaagctc gcttgcgtaa ttactctcag ccgtctgcaa 59460 ccgggaagtg agtaacccga cttcgtaact atggtttctt tggagagcat tgtatttgtc 59520 ctgtaatttt tgtagtgcct tctgatcttc aaccttttgg gcatcccact tcttttgaac 59580 agtagactgt ccattggaat taccccaaaa gtaaatggtg gccccgagga ccaccaccag 59640 aagataaggc caacctttag agattagcat cttcattaca tgcctccagt ggcacattgc 59700 ctattcggtt agctacccaa ccatacgtaa agtctggcat gtttaatgag gtgtagtggt 59760 taagttgttt agcatcaagt aacttaatca ttacctgaca tgctgcaact ttgcctcgtt 59820 ttttctgtaa tgctttatat gcattaacag tacttgtacc gactttacca tcaacctgta 59880 ttttagggta gtctttgcca tcacgggaca tttcattaag agattgctgc aaccacttag 59940 ccggacgggt tacacccgta ttaacaccag catctaccag cttatgtgtt acagcaggag 60000 atatatcagc gaaggctacg aagttaggct taagtacgta atcatcaatg tatatctcag 60060 cagccatctc tttagataaa cccttcatag aaccatccca gccatactca gtagccagaa 60120 cttctttgtg agatttagct acagcttggg taataccatg attggtttca ccacctgggt 60180 cacgaggatt atttacatat cccccttcca tataaaacac tgcccccagg atagcagcga 60240 caactcctcc cactgcacca cctttcgtag caagtttctg tttagctttc atgtgaatct 60300 tcctttgatc ggaatcgtat taatctgcct acaatattta aggcaaacag cgcgattgca 60360 atattggaac catggggaat atcttccagg atgtgacgag gtaatcctgt cagcattggt 60420 tgaatgatat ctatggtaga aaacataatt aacccgagcg tactaatctg gatggatgcc 60480 cacttccagc atcttttcca gttaggtact aactctactt ttctttttag cctgcgaacc 60540 atgcgaatat ctcccgtctt gcagcagcca atacaccaat aattgcacct gccccagccc 60600 acacccactt accaaaaatt ccagcaccaa caactttatg cttaatggtg atgaactctt 60660 caatagtagg ttcattctta gctaagctat cctccacatt ttttagtcta ttgcctatgt 60720 cattgattga atcccgtagt tctaccagag tttcctccag cttctcacga tcctgcctgt 60780 cccttgtctg gttctcaaac agagtcttca atcgttcctc cagtctgact agaagcagtt 60840 cacctgattc attcataaaa ttaccgtagt gatgtagtta gattccccaa cagggccata 60900 tcataagatg actcttagat aatacttagt atgatgggat acatccagac atagtgtctg 60960 gtagcaaccc atggtggatg gtattcagtt aggtatgttt aacatatccg ggcaagtaat 61020 taccctaaca gcagtatcgg ttagggtaat gcaaacagta actctatata agaaaaggcc 61080 caaactgggc cttagtttta attaaatcaa tccgtaaact gcattgtgta ttacgacgcc 61140 cccaacaact gaagaaacat taaaattaat ttcagctttg taggctcctt gtggaacgca 61200 atccaccaaa gcataaaagt tgaacgtgtt taccactggg atgatatatc ctatggaatc 61260 cgcaacagca tttccttgct ggtccaggaa tctaattgac gccagggtgg ttgttgtatt 61320 tttagcccaa aaaccaagct gaaaataacg acccggttca caatcaatga ttgtttgagt 61380 gaagttagct gctgcatctg ctgatggtac agataaaaca aaagaagttg taaacattaa 61440 atcgttagga acatcgtttg ctgtagtagc agtgacagca cctgttccag tttgagagag 61500 tgtccagtta gatgtatttc caaactgtcc tgagccattg ctaatactat taatataagg 61560 gcttacaatg cttcctagat tccagttagt tgtaataaag cctgcacgag ggaagcaccc 61620 tctagacatt actttcttgc tagagccacc tatcaactgt cttgtggcaa atccagtagc 61680 aatcgttgtg ttaccgtaga ggggcaatga gcagttattt aaactaacta ccccgtcacc 61740 attactagca ataggcactg tactgtagcc ttccggaagc agaatagtag aatctttaat 61800 actgaggcgt gagcttccac tcacagtaaa gcctacaaag ctctgcccag gttgatactc 61860 aatattaccg ttagcaaata ctagggtagc attatcactt aatgccactg ctggatcgaa 61920 gtagcctgcc tttttacctg ctggtaatga gcatgaatca aagatgaact gcccattctc 61980 aaaagtaaat ggacccccat tatcaaccat ccagcagtgg ttgaatttta taacctcacc 62040 agtattagcc ggggaattaa agattacaga gttagtaaaa ctgcgagaaa tagtgcactt 62100 atcaaataga atacggtatg cattatcttt aaactcaata cctgcggtag atataaaccc 62160 acagttagat atacgtaatt ctgctatctc ccctccagta ctactaccca aacacatctt 62220 tcgagtacct ttacctacaa aggaaatacc tgttaatagg gcagtagcat taatggcatc 62280 acgagctgtg taatccgtat tagagttgat tatggttaag taatactctc ctgttacacc 62340 ttccagttcc gtaagggtac acttattagc tcctactaaa gcccccttag ctacatccag 62400 taagataggt gtactgatag tccctgatgt aggtacaaga caatcgtaac cggcagagtt 62460 taccttgtta atgaatgggg caatatcccc cgtaccagta taacctgcat catataccgt 62520 agcagtacct tcaaagaact tacgcttcca gaccacacct gcaccggtga aagttacaac 62580 ttcatctaca ggagtagtat taggaaccgc aatcataata cccccaccaa cggaagagcc 62640 tgagtatgaa tctagcatta cactagaccc tactgttgct ccgacagaag ccagggcagt 62700 tacgtcaggt acactaccaa ttaaggtcat accatcctga gatgccaact gagcacgcaa 62760 attaggatcc gtttgagact tccagtccgt gttgccaaca gggttggtcc cttcaacaat 62820 agttacgggt agagaaccta agtaggtata accaatacca ttatgcatca aaagctcatt 62880 tcgagtattt actactaagc ccgtagtaaa tgaatcagat aagcatacaa actctctacg 62940 tgcagcagcc agttcaccca aatcaactgt acctgctgaa tgaaccaaga ctgcatgttc 63000 actgagactt acagcaatgg ttcctgctgg aagtacaggc aatgagtaag cacgttgagt 63060 agtcttgtca taaatgactt tgtatccacc caagtctact cctacagaaa agtagaccac 63120 ttcattctgc ttaataccaa acttacgggc aatggcttgc ttgttggtaa gaataccagt 63180 ggaaccttta ccgccttgac tgaacatctc gttcatacga acctctttgt ttttctatta 63240 aaggtcactt agtgaccctt tgcttaaata atgttaattg gaataaggga aactgtaatg 63300 ttacccggaa cgtaagtgag taaatcccct actacagatt taattctgac attagttggt 63360 agtgtaggga gcttgtatga cttctgctga gaagctacat acacaaccac tttaccattc 63420 aagtaggtaa cagtatccgt atctaaaatt atctcagaat cccgtaagtt gaatccgcga 63480 gctacttcct ggattgttct atcagaaaca taaattattt cttgattgcc cccaatagta 63540 ataattaccc tatcatcggt tacaagtggt tctgtcagga agattctctt attctctgca 63600 tcgacagtgt atgacttatt gggcaaacct ttatacaatc ttgccccatt cttatacaca 63660 gcaggtacat caatgaaagc gaatgggata tctataacct gctcacctcc tacagcatta 63720 ccaaaattgt ataaccagtt aacaaccttc cagttatcaa tagcaacagt atctggggaa 63780 gcaggcacac ctgtaaggaa cataataact tcgtcacctt cttccagttc ctgagctaat 63840 ttaatctcag tatcaccagt agaatatgtg aaacccttcc cattgagctg tgtgaatccc 63900 cctactgtaa taaaaggtat gccaacggta tcaaactcaa ctggtataga agtttcccct 63960 ccagcagagg tatacccaac aactatatag gtatttgtat tagtggactc actaccactt 64020 gaggtcatgg atacttgtac ccaggaacct gtagctgccg ggtcatccgg tatgattcca 64080 gatttatcat catcaacata taagtagtcg gtaatggttc ttttattact acggttcctg 64140 ccataaatgc gtgcatactt tgttttcaga gggagtgatt taagttctat ttcagactgt 64200 acaccaataa ccatcccgta ctgatttaag aaatcataca ggagtttaag attacctaag 64260 ttacagtaga cagtacgaac cacatggtaa gcattaccaa gtagtgcatc taccattggg 64320 tcattagaac tgttctgaca gtaaggatta gaccaatcct tctttgcatc aaaggggttg 64380 tgtgcgttca tcgccatcct cgttgttcaa atttagtttc ggtaattgaa atacttccat 64440 tagccagatc gtaatcaaca acctcaccac agatgctttc atatgtttgc agatactctg 64500 ctgccttagc attagcttcc ggagtattca aaccagtgtg gtaacgataa cccacccagt 64560 tatgaagtgc cgtcattaac gtatctgcta agtcaacttc ctgttcttca tcacccgtaa 64620 gtactgggtg cttagcttga taagttacgt taagtgcctc aaagtgtcga ggtcgcatac 64680 actgaatagt atcaggccga ggggtgaaaa ttgcatgagg atctgagtcg tcgttcagtc 64740 tacgtcgatt ccctgagtta tcaaacacat ttaggatttt aataacgtca tcctggaaag 64800 gtttcatgaa cccatccatg atgtatggat attgaatctc aagagtaggc ttagtaaatc 64860 tggagtaagc atatttggat tgaaggggat aatcagtacg accttccttc atctccacaa 64920 tgcagctatt agtcctcaga gggaaacgac tatgcagtcg taccagtcct tcattaatag 64980 cagccaatat ctctggctta ctatctggtt caatatctaa tctgtcatca gtgactgcac 65040 cagtaccttt taaggtactt agtgccaagc cacggtatac ttccgataat ttctgcatgg 65100 ttcctcacac aatgtatgaa cgtagggggt taacactatc ttcttcttca tcatcccaca 65160 ttgggtcacc cttattatcc accatagtca tacctgcttg tggtttccaa ggattgagat 65220 aacccagcat ggagatagta tcaatacagt catccttacc tttgattcca ttaatggtag 65280 ctagtttaat ctggcccatg aacagaccca taatagttga atctctcaac tcttccggga 65340 agtacatctt accagctttg aaccatggaa ctaccaggtt aaagcgtgac agcttagagg 65400 ttacaggacg tatacccggc ttaccacctt cagaagatgc aaagttaaag aagacattac 65460 ggttaatcat ttctttctga aggagcgaaa tgaatccacc ttgctgccct gtgatttcga 65520 caccaacgtt ttgtggttgg tattcttgaa ccagacggaa caggtcatca aagtttttat 65580 ccataagctg acgattagcc acaccatcaa cccagaacca atctccatta gaactataag 65640 cccaaactga tatgacacta tagtcactgg tctgtttctc cgatgtagca aagtcagttg 65700 taatataata gttgtagcag gacttcatcc ttaatagttg ctgtctgcta taccatctaa 65760 tttcactatc ctgaaccagt ctctcatctt cagagctaat acggagcata agttcctgat 65820 agaagcctgc cagcttaccc gtcttaactg ccatgtcata ttgggctttg atgtagtcgt 65880 aggagaaacg gtcatcccat gcaccttgaa attcttccct actacaaggg aacttctcac 65940 acacaggcca tacgttgaca tcccatgcac cagactctac tgcttcaatg ataatatctt 66000 ccttattaaa gggagtacca ttgaagatta ctttacgacg ggtaggatca agagcatggt 66060 tcacaccttt atagacggta tccttaatag cttccatact cgtcttggag ttagcatcac 66120 catcactaat caagtcatcc agtacgcata atgttggacg tttaccatat atcttcgtac 66180 cacgaagacc tgtcttagca ccaaacagct taacacctag acgatgtcct tctacattac 66240 gaaactctaa caggttatcc gtaaaggtag cttcaggtat ccattgctga aggaactcac 66300 tattcttgta acgaaactca atgttcttac gtgcagattt agcaccgtta tccattgagt 66360 cagatacata aatcattcct tctactttac ccaggcttgg taaatgcccg aacactgcca 66420 ggaacaatgt aaagtattcc atgaatacag cagtcttacc tgcaccacgg aagcacagat 66480 taactacata ctggttcggg ctaatcatct tatccaacat tttcaggtga actggaggtg 66540 ttttgttgga ttcaccctct ttaccattaa ccaacttaat aaagttggca aaggtaagag 66600 caaactgact aggaacatag ttggaagaat taaggtggga gtaatccacc tggtctagcc 66660 attcatccag ttcctgctta attaactcag acatctgtaa tgtcctcatc tgcacgtact 66720 agtttagaac cagcaacctc tttggtaggt acaccactat tgattgcatt aatttgctgc 66780 tcagctaatg cagcaagtgt tgccttgagg tcagttaatc cagagttctc tctcaagtcc 66840 agattgatat tcgttacctg gtcttttggt ttagctaagt gggtaaggat agagttagct 66900 gcatcacatc ttaccttctc acttgctgct gtagtcatca gttcaacctg aacattgatt 66960 gccttctgat aattatcctg gttcacaatc cacactggaa ccaaactttg ttccatgatg 67020 aggttaacta acttacccct gtgataagca gatacataag cactaatatc tttctcgctg 67080 gttcctcgtg caacaagttc tgcttgtcgg ttagggaatg tcttgaagta ggcttcctta 67140 ttggaataac ccatgtgttt ataagtcaca tactgaactg cattcatgta gtcctgtgtc 67200 ttaaacttac cttccttcat tacaccagag taggagataa agttttcacg gaaggactca 67260 gcaaccagtt ggtcttgggt tatgttgttg atcgtgtcta ccaactcctg ggtcacactg 67320 tttttgaagt tagcaggtaa ggcattaaca atctgctgct tagttagttc actcatactt 67380 atctcgctac aaagttatgt ctattaaaga agattctttt cttggggtga gttacccctt 67440 gaagaaacga taccatacag ttacacttta gtcatgatat agaaaaagtt catgatacta 67500 aatagtatgt aatgttccga tgaccctatt cccaaaagga gtaacctttg agaatctata 67560 tgaaagcccc agactctagt gacaagtact tcgaactaga ccacactaag attggcttcc 67620 tttcagatga tattatcatt acacatgatg aagacaccta ccatgaatta gctgggatga 67680 tgctgttaga gaatcgtcca ggcattaaag tcttagaacc aagagaaact tactctttag 67740 ataaaacaat ttcatctctt gtgttcttca caaaattacc tcatagtgtc tccgtcaggc 67800 gaagcaaaga cagtgttcgg atgacggtga cgatgtgatt agtcatgccg cctagtagac 67860 agtggcggta ctcgtaaaga gttaataact tctacagtca gggttgcccg gctcctttcc 67920 tacctagtag gctaacgata cacatgtatc tagggtgatg aactgacccg tgattgaaaa 67980 cggtaagggg tagagtcgag aggctctacc ccatttcttt ttctggagga atgcaattat 68040 gagcatctac gcttttgata ttgatattac cggagtactt cataagcaag gaccttatct 68100 acttggggaa ccagaaacag taagtaagtt cattgctccc tggcaatctg tgttcttacc 68160 taactgtgaa gagtcccatg agattgttgg ctgtcttttc ttatcaaacc tacaactcaa 68220 tactgagata cttaccgaac caaaggtgta tgacctcact cgttatgacg gattcaatga 68280 gttcttccct ggtatacgtt tctataaaat acccggttgg gaattgatag ttgaccataa 68340 cgtctttaat aaaacacttt ccatttactt cagaccactt tcctaaagga aggagaatca 68400 atcatgaaat tcataatgac catccccagt tttgctgaaa gggagtctat gtatacaaat 68460 gacttctctt tagaagaagt catgaatagt agcccccaac atgtaatcct agttcctacc 68520 ttagaggact accatgaagt aaggggccag ttattcttag caggtaatga ccgtaaggtt 68580 gtatgtattg tcccaccttc cgtctactcc tttagtgatg catatgacgc tctacctttc 68640 atgatggtat ccgatgaata ccaatgggat agtcgccata agaatgaaga ataccattac 68700 tactatgtaa ggaagtaagt tatgcacttc ttcggtttaa gtagtcgtag tacatgtccc 68760 cttaatctgg gagtaggtaa atccccctgg ttgggaataa gtaaacccat gaccatgtac 68820 tatgaaccct gtaaagaagc agatgagttc ataggaacct gtctaatgtt aggtgcacct 68880 attaccttta ttaagataaa tacttccatc gaagttccag aggatgtcgc tgtatacatc 68940 ttctcctcca gtgaacctat ggtagctact actcttacag gcagactcat taccctctat 69000 ccttcctcat atttatctag cgacggaagc tactaatggt tatcagtaaa atttattcag 69060 agctacctgg ggatgaatac acccaccata aagaattaac taacctggat gctgatgtac 69120 tcatactggt tccagacgaa cagtccttgc atgaactaca aggattactc ttcttataca 69180 ataagaattc ggaagtacat ctacttgaac caggaacacc gatgtcctgg cagggtagct 69240 tcagtccttg gcatctcaat acggacaagt acatactatc cttcgaggat aacaccccaa 69300 tctataagga aagacaatga aactaaacac atcaccctat ccggtgcagt tagaagtagt 69360 actcgaccgg gataccttca ttaaaaagta caagaaactt aaagggtatg agccagattt 69420 ggaaggttgt aaggggtata ctacatactc tgataataag gttcttatgg gcatcttctc 69480 agaccccttg ccaacgctta tccatgaagt aaaccacttc tgcttatggg tattcgatta 69540 tattggtatg cctataaaca gtagtaatag tgaagcgtac tgttactaca tggatagcat 69600 tcttgaacag gtactgaaga atgaacgtta aactattagg tatgaaaggt actgacttac 69660 tgcactattc ctctgaacca gggaaagcaa tcattcacat tatgtcctac ggaacctcaa 69720 cgctaataat ggagccagga gagttagcag actttgcaca gggacataag ctaattacta 69780 acagtaatgc agagatagtt atcctgcaaa atggtgagtc agtattcata ccatacggtg 69840 attatgccta tgtat 69855 <210> 2 <211> 77315 <212> DNA <213> Artificial Sequence <220> <223> Escherichia phage KBNP135(ECBP2), complete genome <400> 2 cgcactgcgt gcaacgctga aaaagaaaga acataaagaa agtattaaaa gaataataat 60 aacatcaatt atcaatacca atctaattaa tacatacatc attaatccac ccataatcat 120 tacattcaga atcatttaca ttcaaaccgg gccaacctgc aaggcagttg gtcagaacta 180 tggggcgttg caatggttgc ttactggtat taatagcttc gatataatca agcctttact 240 agttcgtcat cagggctggc ctttcatccc ttctcaacat gagtaagaga aatacgggta 300 ggaaatcact ggttagatat acatgggtaa ataatgcatg tctaatatca atgggtaggg 360 tatttatggg taggcatgcc actgatagca ggtagggcaa ggcttagagc ttaaaggctg 420 attgatttcc taaagacgat caccagcaga ataacccttt tttatttgag gactccccct 480 ttttaacaat tcgccgcaga gcgcctagcc ccactcggtc aaatcagaat tttaaacaga 540 tatatataag gttgaatatg aatctaatta aagtatttag acggatgaag tgtaagcaca 600 attactatcc tcttcgtaaa gcttacgggg atgaaagaat acatggtctc tacctatcaa 660 catggaagtg taaggaatgt ggtaagttga gattcagtac atggatggat agaccttaat 720 ggaaataatc ttttggttag taatagtctt ctgtttattc atctgcttct ctatagcaag 780 atgtatcaag tatgactaat gacatggcct taacatgagg gtgcctttat gacatgccct 840 ttacttgaac gtagtgaact acttcagcgt acacgggttt atacatgggt gggcctttag 900 atgaatacgc ctttatgggt tgacccttac tcagaggaga acaaatatgt ttctagactt 960 tttatcagag ttgattcctt actttcgcac tgagacatgt gcattcaaag ttgaagaaga 1020 tggggacttt gaagtaataa cttgtttggg tagattggta gacctagacg acgatgaaga 1080 ttttgatgtg acaatagatt tgagttcatt gggttggttg tacatgagat tttgcataac 1140 ctacaaaccc gagacttata aagattatcg ttataccact gaaggagaga atgatgaata 1200 acaatatgac aggaactaat acactgaatg caatctatga gaacatggga tgtaaaggcg 1260 aggttgttta caatggtaat ctccgtcctt tatcagagga cactctctat ctgaatcgtg 1320 ttcatgaaac cactggaggt aaatcccgag ttgagatggt aaaacaattg gctcatgatt 1380 ctgtgtatgg gtatgctcct tcaacccgtg aattatggga aggtttgatt cctcgtacag 1440 aagctaaaga tgtgaaagat tatttcactg tagcaaagga agttaaacgt aagcttcctt 1500 ccaatctaga agttgttccg tatgtatctg acaaacatgc acacctgccg gattatgccc 1560 gtgagatgat tgagatgatt gaagaagccg agtatgaagc tctgaataat tatctcaatt 1620 caaaagacca atggtctcgt aatgtagtaa ttgatgaaga aggtaatgaa gtagttacgt 1680 gggtgcatgt tagtgaacaa gctctggacc ccgagcttga agaatacaaa aaatttttgg 1740 cgtcctcttc ttttgatgta gagaatgaca agtccgttcc agttacttgg aaaggtgaaa 1800 cggtagctct ccagactttg ttcaacctac acgtaggtga tgtagactaa taaaggtgtt 1860 gcctccttca gtgggaggct ttttgttata acgataatgt agaaggagta tacaatgttt 1920 ggagaaaaag gagaactcac gcctacacag gtagctgagg ttacaggaag attacccggt 1980 gtaactgatg aattggaaag ccgtggtatg gagccaagca cagggaagtt ccaaaaggga 2040 aataagttcg gacgacccaa aggtagtaga ggtaagttga cacagctcat gttggaccga 2100 gttgctgcaa gtcatctgtc ccctgatgaa gtgctgattc aaatttatga agacccaaat 2160 attccacctg atcttagatt caaagctgca agtaaagttg cagatctggt atatccgaag 2220 gcagcttctg ttgagattaa gattgaggat gagaatgtaa ttaacgagga ttcgttgaac 2280 gcacaaatca aggacttctt ggctggagca ttaggtacag atgtgctacc tccagaggac 2340 tctgaggacg ctgaaaagga ggaggaagcg gacgagagtt aattaaagga aagggttccc 2400 cttctagtaa agcgctagga gatttgttac cctgtatgaa gaggggaggg ttcttttcgc 2460 gagggatctc tccctttttt attttcaatt ggaggaaaca tgtcattaga taaagaaata 2520 tatgaagcac taactggaga caagcttacg acagaggaga aaaaggctct gcttgaaatg 2580 ctgaaggata gagatcaatg gagaaagtat aataggattc tagctttcaa agcataccag 2640 tttcagaaag agttttatgc agcaggattg aagcatcgct ttagattctt gtgtgcagcg 2700 aaccgtgttg gtaaatctta cagcgaggca tacgagtttg cttgtcatgt cacaggtcgc 2760 tatccaacgt ggtggacagg atatagattt aatcgaccca tattggcttg ggcagtaggg 2820 attacagggg actctacaag aaaggtattg caaaaggaat tgtttggtac accaatcgga 2880 aaggacacca acctaatagg aacaggagtt attcctcgtg atgcaattgt catagacaca 2940 attgaacgag atggtaataa attacaaatc gtacaaatca agcaccaaaa cgctagaggt 3000 gaattcgatg ggctgagtac gttagaattc cgttctaccc aacagggtga acacacatta 3060 atgggtgcta cagtggacta tatttggctg gacgaagaag atccttacga aagtatggca 3120 attttctctc agtgcgttac acgtacactt actactaaag gtttagttac tattacagca 3180 acaccagaga acggtttaac agagctggtg gacaagttta tgaaaggtga aggggacgag 3240 aacacaggtt ctctgtattt ccagaacgct tcttggtggg atgcacatgt cgatataggt 3300 ggacacatct ctgaacaaga tattaaagat atgactgaag gtattccagc gtggcaatta 3360 gaaatgcgct ctaaaggtat gcctctgttg ggttcaggtt tgatttacga tgtatctgat 3420 gattcaatta agtgtgaccc gtttgaaatt cctgagacat ggaaacgcgt atgtgccatt 3480 gatattggta ttgatcaccc gacggcagct gtatggtcag catacgatgc gaacaccgat 3540 acaatctacg tttatgactg ctataaagaa agcggcttca ctccagtgta tcatgctcct 3600 gcaattaacg gacgtggaca gtggattcca gtgatactac ctcatgatgc ggacaatacc 3660 gagaagggta gtggtgcttc tgtagctcaa ttctataaaa atgcaggtgt taacgtacaa 3720 agtgagacct tccataacaa gatcggaatg gatggtaaga agaacttctt tgtggagccg 3780 ggaattactg atattcgtga acgtatgatg tcaggtcgat tcaaaatctt taatactacc 3840 gcatgtgcta agctctttga agagaaagca cgttaccatc gaaaagttgg taagattatt 3900 aaagaacatg atgaccttat ggatgcaatg cgatattcag cgtgttctgt tacacacaga 3960 ggtcggtcta agcatgacgc tagctatggt agctcgtcgc tttatcaaga aaatattaac 4020 agatggaatt cgagttacta aatacaagtg tttctagtga gggttaagtc agttattgcc 4080 ctcattagct tatggtatca gcatactgac atgctgacgt gaggggagtg caacggcccc 4140 tcttagtaac tcattccaag ctatcaccga gggagagatt agatggctaa gaataaattt 4200 agtgaagata ttttggatga actgcgcgtt gaccttcaac gtcgattcta ttatgctcaa 4260 ggttatgtag acattgctgt aaaaggctat gctcgtgaag cttgggaata cttctatggt 4320 aacttacctg ctccagttac agatggtagc tctagttggg tagaccgtac agtatgggaa 4380 tcagtaaacg gtacattgca agatatcatt aacgtattct gttcaggcga tgaagcggtg 4440 acgtttgttg cggacaacca agcagattct gatgcagctg atgtagcaac taagcttgtc 4500 aaccaaatca tgcttcgtga taatccggga tataacatca tttcctcagc ggctcaggaa 4560 tgtctggtta cacgtaactc ttttatcaaa tactattggg atgaacaaac ctcaactcaa 4620 actgaagagg cagagggcgt tccaccggaa gctcttgctg catatgttca aggtatggaa 4680 gcaggtggtt tacaagattt agaaatcttt accactgaaa atgaagatgg tacggttgac 4740 gtcaaggtca cgtacaaaca aactgttaaa cgtgttaaag ttgaatatgt tccttcagag 4800 caaatctttg ttgatgaaca tgcaacctct tttgcagatg ctcagtattt ctgccatcgc 4860 acacgtaagt ctaaagatga attacttgct cttggtttcc ctaaagacga aatcgaagca 4920 ttcaacgatt ggactgacac aatggatacc actcaatcaa ctgttgcttg gtcacgtact 4980 gactggagac aggacattga tgcagatatt ggtgtagata ctgaagatgt atcttctatg 5040 gtgtgggtct atgaacacta tattcgtact ggtgtactag ataagaataa agaatctaaa 5100 ttgtaccaag taatccaagc aggcgagcac atcctttcct gtgaagaagt taccagtatt 5160 ccttttgtta ctttctgtcc ttatccgatt ccgggttcct tctatggtca atccgtgtat 5220 gacattacta aggatattca agacctccgt acagctttag tacgtggtta tattgataac 5280 gttaacaatg ccaactatgg tcgctataag gcgatagcag gcacgtacga ccgtcgttct 5340 ttgctagaca accgtccggg tggtgtagta gaaatggagc gtcaggatgc gattgatctc 5400 ttcccatacc acaatctacc tcagggtatt gatggtctgt tgggtatgtc tgatgaattg 5460 aaagaaactc gtacaggtgt tactaaactt ggcatgggta ttaaccctga tgtatttaag 5520 aatgataatg cgtatgcaac tgttggctta atgatgaatg ctgcacagaa ccgtctccgt 5580 atggtgtgtc gtaacattgc acataacggt atggttgagt taatgcgtgg tatttataac 5640 cttatcagag aaaatggtga agtcccttta caggtacaga cccctcgtgg tatggtagaa 5700 gttaatccta aacaattacc agctcgtcac aatctacaag ttgttgtagc tatctctcct 5760 aatgagaaag cagaacgtgc acagaaactg attagtctga agcaattgat tgctgctgat 5820 gctcaacttg cacctctctt tactctggaa caagaccgtt atatgactgc tcagatcttt 5880 gaactgatgg gaattaaaga tactcataaa taccttatgc ctctggaact gtatcaaccg 5940 cctcagcctt ctccgatgga aatcctccag ctcgagatga ctaaagctca ggttgagaac 6000 gtacaggctt cttctcagaa gatgattgct gatgcattcg accagcgtga acgtactaca 6060 ttcgaacagc agaaagctgc ggatgacttt agtttacgtc aggaagaact gcaattcaag 6120 caagaaaatg cagcagacca tatgactctt gagaaccgta aggaagacaa ctcagctact 6180 cttgaacaag ctaagcataa gttagctctg ctccagcaac aggttcgtca gtacgaatca 6240 gtattgaaag aactacagtt gaccatgagt catcaagttg atttggagaa gattgaccag 6300 caagctcaca ttcaggataa aactttggaa ctccaaaaga aagaagcaaa tgaaactaag 6360 aaagaacaac aaagttctct caaagattca cgtattccta atagaaagct tggtagtaga 6420 aaataaggac acggaatgaa tactagacat aaatttaacg aagagcatgc cgccgctttg 6480 gcggctctca atcataacgg cattcttgac gcagcattag aggcattaaa agaaacactt 6540 atgagagata tcgtacttac cgacgttcat gagtccaaga aacgcgaaga gtgttatcaa 6600 aagtataaaa tggttggtag cttaaaagag gtcataaaag ctgctatcaa tagtgcagga 6660 gaataatcct aatggcaaat gaatttattg acccaaacga tttcaaatca ttccttcagt 6720 tagatgactt aaaaggttcc ttcaaagaag accttagtcg attagaatct gaacaagaac 6780 cagaagtgga cattgacgat gaattcccgg aagatgccgg tgaccagttc gacattgatt 6840 ctgacattct gggcaaaggc gatagcctag cgttcgaaga tgaagacgga tgggatgatt 6900 cggaagagga aggtgaagaa gacgaaggtg attctgacag cgacgctgat gaagtggatg 6960 tggaagatga gccaagtgat gcagatcacg atgatgctgc tgacagtgac tacgaagatg 7020 gcgatgcttt cgatgtagat tatgaaactg ttatcaccct tcctgatggt cgtgagatga 7080 ctattgaaga gttatcaaat ggctatgtta ctggctctga tatgactgaa cgggaatctg 7140 ttcttcagcg tcatgttgaa gcttttgaag agcgtgtagg tggtttagcg gatgtattag 7200 aattagcatc cttagaagca gaccgagtga ttgaagatta caacggtttt gattgggaca 7260 agctagcagt tgaagatcca caagcctatg ttgaaaacaa acgcttttta gagaaataca 7320 ctgctcgtcg tcaacagctt gaacaggctc aggtcaaaat taagcaagat gcacaagcaa 7380 aggaacaaca agccttccaa gccaaatgta ctgagtgtgt taatgtattg aaacgtgaga 7440 ttcctaattg ggatgacagc gtttatcaga atctgatgca atatgcaatt gatctcggtg 7500 ctactgaaga agaagtcttg aaagagaacc gtccgtcaat cttccttgct ctgcacaaag 7560 cataccaatt tgataaaggt aagcagcagg tgatggctaa gattaaacgt cccggtgctc 7620 cacgtaaggt tgtgaagtcc gatgcttcta agtctcgttc ttccaatacg ccagacaacg 7680 ctaaggttgc caaggcattt tcagagggtc gtattcgtca cgaagatgca tttaagtatc 7740 tcgtagatta atcgacaaat tattttttaa tagtatagga gctaacataa catggcaaat 7800 ccaactttat ttgtatctta cgaccagaat ggtaaaaaac tttcttttgc taactggatt 7860 tctgttctgt ccccgcagga taccccgttt gtgtctatga ctggtaaaga gtctatcaac 7920 cagactattt tcagctggca gaccgatgca ctggcaagtg ttgatgctaa caacgcacac 7980 gttgaaggct ctcgcgctga agacggtgaa atgaaaccga ccgttatcaa atctaacgtt 8040 actcagattc tgcgtaaagt agttcgtgta tctgataccg ctaacaccac cgctaactac 8100 ggtcgtggtc gtgaactgat gtatcagctg gagaagaaag gtaaagagat taaacgtgac 8160 ctcgagaaaa tcttgctgtc cggtcaggct cgtaccgacg ttctggctga ccagtatctg 8220 actaactcag cagctgatcc tgctcagggt ggcctgaatg acactcatgc agctcgtaag 8280 actggtgctt tccagttcct gtgtgctcac ggcggtctga ctggtacgaa cgttaacaaa 8340 actgttgatg gcccggctga cccggatact ggtgcagtaa ctgttaaagt tgctaagaac 8400 gcatctaacc cggttgctaa catcggcttt gatgaagcag atatctttga tatgaccctt 8460 cagctatata ccgcaggttc tgaagcagac atcatcatga ttaaccctgc tcatgcagca 8520 atctttgcta agctgcaaga aaactccgtt ggctctcgtc agcgtatctt cgaaaacacc 8580 aagcagttca tctacgaagt aaatagcatc actgacccgc tgggccagag ctataagatc 8640 gtagttaacc gttggatgcc tgctgatgca gtttacttct tccgcagctc tgactggact 8700 cagatggtcc ttcgtgctcc gaagcgtact gaactggcta aagatggttc ttacgagaag 8760 tggatgatcg agatggaagt tggtctgcgt caccgcaacc cgtatgcttc tggcgtactg 8820 ttcaccgctg cgggaaaggc gggagcgtaa cagttgacag tgtaactgtt agtcctgcgt 8880 ctgttgctag tcttcagacg ggggctactc ggcagttcac tgcaagcgcg gagaaaagtg 8940 atggttctac cgaaacaact ggctttactt ggtcagtgac aggaggtggt tctatcactc 9000 agtctggttt gtatactgct ccgagtgcgg ctcaatccac tgatgtgtcc attactgcaa 9060 cattagaaga tgtttctggt acagcaacca tttctgaaat tgttgctcct gcccctacca 9120 tcgtatctcg tggtccaatc gcagataagg taggcggttc tactacagct ttcactacaa 9180 tgtttaccgt taccaattca gctgcttcta actatacttt cgtagtaact cctccgggag 9240 ctggttctgt aagtgctgcc ggtcttctga ccttggctga tgctgcttct ggtgctgtaa 9300 gtgttaaagc cacccactct acggtttcga gtgttacggc tacttgtgaa tttacaggtg 9360 taactccaaa agagtaaaac tataaaggct gccttcgggt ggccttttct tgttttaaga 9420 cctatacaag ccagtgtgta atgtatattc tttgtctaca tacaactagg aggacaatct 9480 atggcgatgc cagacgttca aaacccaatt aatacctacg gatggcttaa gaaggctgtg 9540 tccctctggg ctgaccgtga tgaccctgaa tttgttaatc aaattccaaa ctttattaac 9600 tttgcagaaa aggaaatcta tcgtaactta cgtattcctt ctctggaaaa agaagtatat 9660 ttagatatta aagatggtgt ggcttttatc ccaccagact tcttagaagc acaatggatt 9720 atgagagctt cgaatgggat gatgttccga gcaacctctc ctgaagaact tgattggatg 9780 aaaagaaata actctattaa cccatccaac tggaatcaag gtgatgtgat gtttgctcgt 9840 ttaggtagtc gctttatgtt ctacccactc attgatgcag acactcctat ttatcctgat 9900 gatggagcac ctgagattcc tgctgataat gctgtggttt tgagttatta tgctgaccct 9960 ccagaattcc atgaagacga agatacaagt gctattctga ctattgctcc tgaattactt 10020 ctctatttta ctctacgtca tgcttctttg tttgttcaag atgataatgg agtgcagaag 10080 tggtctgctc ttggtaaagc aattctagat gaaattgttg agcagagtaa gaaagcagaa 10140 tactcagggt cacctcttgt aatccctcga accattagcc gtcttaatag ctctcgtgaa 10200 gtgtacggca ttcgtagaaa cagataagga gaacctatga tcgtttataa caaccaagaa 10260 cctaacgcag taaataacgt aggtcaattt ggtgcaacag aaggctctat tggagcttac 10320 aaacaagcag ctgagtatgc tgctgatgct aagtattggg cgctcttagc tgaatctaaa 10380 tttggtacag ttgatgacct tatcgctgaa gtagagaggc tctacaagca aggtatcctg 10440 ttacagcaag acattgaaga cttgaagcaa gactttaaag atcaagatgc tcgactgatg 10500 caactgattg cacagactaa cgcagcagta gctaacgcta acaactccat tgctttgatt 10560 aaccagaaac tgattgaagt tgatcagcag cttgacattc tgttggctat gaaagttaca 10620 gtgactacac ttcctccggg ttctgaagct actggctctt tcaacaacca gactggcgaa 10680 atcaagcttg ggattcctga aggtgagccg ggtcgtgatg gctccgttac tgatttaaat 10740 acagcaccat ctggaactcc tgttcttggt gatatcggtt tctatgtaga tgcggatgac 10800 aataaagttt acaaaactac tcttagtgat attgctaacc tgatcccttc tgtccgttct 10860 atcaaagtta atgatggtcc agaacaaact ggagatgtag ctctcactct taataaaaac 10920 acggttggct taggtaatgt tttgaacgtt gctcaatata gtcgccaaga gattaatgat 10980 aagtttgata aaacaactaa aacataccaa tcgaaagctg aagctgattt agatgcccaa 11040 taccgacagg taggagaaaa ggttttggtg tgggactcaa cgaagtatga attctatact 11100 gttgcggctg ataagaccct tactccagtt aaaacagaag gccgtatcct aactgttaac 11160 tctcgtgctc ctgattcaac tggtaacatt gacattacca ttccaacagg aaacccttct 11220 ctatatcttg gtgagatggt gatgttccct tatgacccaa cgaagaatgt ctcatatccg 11280 ggagttcttc ctgctgatgg tcgtctggta tcaaaagagt ctgcttcaga tttaggtcca 11340 tcccttgtca gcggacaact ccctgttgtt tctgaaacag agtggcaatc tggttctcgt 11400 caatacttct cttggggtaa attggcagat ggtataactg atgcagattc cactaacttt 11460 attaatattc gtctgccaga ttggacagga ggagaagcaa tacgttctcc agattctgta 11520 aaagatagtg catataaagg taatgttcaa agtcaggtcc cttatgtagt cactataaat 11580 aaccaagctc ctgacgaaat cacaggtaac gtcaacctct ccagatctat cttaggtgct 11640 gctgcttctg gtgcaaactc tgatataaca tctctcgcag ggcttacaac agcgctttct 11700 gtcactcagg gtggaacagg tgcaaagaat cccaatgatg ctaaggtaaa tttagattta 11760 gacagaatag agcaaggttc tggggagact aagatcttat ctccaaataa agaaacctac 11820 atgtttgttg ataataacgg atggggttgt taccgtacca cggctgggtt ggtcggtact 11880 gtagctttgt cagttgagag agggggcact ggagcaacag atgttgctag tgcaagagat 11940 aatctaggac ttaaatctct ttctacatta gatattgttc cagtagcaaa aggcgggaca 12000 ggtgctagtg atgctgcgga ggctaggtct aacttgggtt taggttctgt tgctactctt 12060 agtaatgtac caatctccag tggtggtaca ggtgctggtg atgctgctgg tgcccgaagc 12120 aacattgggg cgctgagtaa cactccagca aacacaggta cgggcggtac tggtgcgcga 12180 gtccaacatg ccgccggaag tggtctgttt actttagaca tgtttaactg ttactggtat 12240 atgcagccag aggatactaa cttctgggtt gcccacggtg tgtcatatgc aggagctggt 12300 ggagaagcct cagattacgg tcgtgtatct tacgcaataa aggttgcaga tggaacaaca 12360 aaatatgtcc attgtcttac aaacaaaaat accattattg atacaggtgg atttatcaaa 12420 gcggcatctc cggtagttaa tatttatacc gatggtcgat atgaaacgaa tgatgaatct 12480 gatggggtta gtgtcatccg gaagggtatt ggtgaatatc tgattactgg ttgcctcggt 12540 cttaactctg atgcaagatg gggtggggtt gatggggggt ttgaaatccc aacagatcgc 12600 aataagcagc ctaggatttg gctggattat gaggttaaag aagatggttc tattttagtt 12660 aaagcctatc acagaaccca tccaacctct ccggtttttg ctagaaatga gatagaaggg 12720 ttttctgatg gagatcctat tgacatacct aaagatgcat tcatctctgt tcgtgttgaa 12780 atgccgtcta agtaaattta agaatgccct cttcggaggg ctttatggag gattataaat 12840 gtctagagaa ttaatgccca aatctgggat aatgatgcct catgttgtta ttaaccgaga 12900 tgctgctgtt gtaggtgttt caactgttga tggtgtagtt ggtgctgtca acctaacgaa 12960 taagtatttg cagaagacgg atgctgaaaa cacctacatg actaaagctg atggagctac 13020 taaagtttat gttgcagatt ctattgctcc tattatggaa ggtgctctat ttaaagctga 13080 cccattcgta gctaatgatg tacctttccg tgctgctggt tctaatggtg tagaatctgt 13140 tgacatgatt aaagtaacac cagagaatac tatcaagatt ggcagttatg cttcttctgt 13200 tcaaggtgta gaggtccaca ctgctggtcg tcttcaagtt gtagatttga atgatggtgg 13260 tgtagaaact aaatacccta tctactctaa acgatatcgt cctgagattg atgaacttcc 13320 ctttgcagcg attggttctt atgtcaagga ctctaagggt cgcaccattg gtgtaaatcg 13380 tactggtatc aactctgata tcaaacagct cactcagaaa gtaaccttta cacaacctgt 13440 aactgttcct gatgcagttg gagattatga tgcagtaact ttgagacagc ttcgtaatag 13500 tggtggtggt tctggtggtc ctaccatgag cggtatctcc aactttggta ttggtgattt 13560 ccatcttcgt gacagtcgtg ctttcattcc tgcatttgaa gttgtgtctg atgggcagct 13620 tttaaaccgc gctgactatc cagatctgtg ggcttatgca caattattgt caccaatcga 13680 ggactctttg tggctttctg acaagttcaa gcgtgggaga tatagtaaag gggatggttc 13740 tactaccttc cgtgttcctg ataggaatgg cgttcaggct ggttctattc gtgctttata 13800 tgctcgcggt gatggtggtt cttcttctgc taacggagat atttttgaat cagcagcacc 13860 aaacattaca gctgatgtga cctcgtattc ttcaacctca tactcacagg tatttgggat 13920 aactcatggt gctatgtatg tagacaacag cactttccca actggtgatg gtgatgctat 13980 cccttcaact ggaaacattg ccatcaaagg tcgattcaat accttacacc tagacgcatc 14040 gcgatcaaac tcaatttatg gtgcatcatc tgatgaaatc atgggtcgta actttgttgg 14100 tgtttggact attcgtgctc atggtggttt tactgctgct aatacttctt ggtctgttat 14160 taacagcgac tcgacagagc ctcctagcgg tacaccgatt actggtggtt tagtttcttc 14220 taaatactta gttggaggtg ttgataagta tcggtcttct ttccaattgc aagggtctaa 14280 tgaagttgat ttatcaacta gaattaccac cataaatgat aaatattcca tcggagctgc 14340 aacttgggat tttaaattag atggtaaact tcttttcaac aagagtctaa ctcctaatgg 14400 aacaggaaac gctcctggaa atacctactt aactctagct aatacttgga tgagttcttc 14460 atactctgga tatgtagggt ttgttggtgg tggggctggg gtttctaatg ggggttggag 14520 aaactttatt tctttagggg ctctaatctt cccagactct aaccatccat ccgctgttat 14580 ttctcaagtt tatgactatg accttcctac aggaaatcaa cctaatggag acattgtcag 14640 aaatacctat ttcagtgctg agagctacga cattaccttc gggaacaaca gtgggactac 14700 taattacatc ttcagtaaat ctccagtctc tgacgaacga ctcaagcata gcattaagga 14760 agaagggaca gccacagctc tttctaactt aaacaagatg gagtataaaa cctttatcta 14820 taattatgat gagaaagcaa ctgtaaggcg tgggtttatt gcccagcaac ttgaagctat 14880 tgaccctcaa tatgttagga agtataaaac tttcaaaggg actgataccc tagctttaga 14940 tgagaatgtg ttgcttttag atgcaattgc tgcaatccaa gaacttacca aaaaggttga 15000 agttctagaa gcgaagttag cagagaagta atctaatagc cccttcgggg gctttagtag 15060 taagtctgtt caagaagtaa tgaaactact atatcacaaa aggaatacac ataggagata 15120 cgaagatgaa aatgatgaaa caaatcagtt atgggttagc ttgtgttgct accggttttg 15180 ctgtgtcagt ggctgttcct gttaacgcag tgcatcctct ggtaacacaa cctgcaccag 15240 ttgagaaatt gagtgcatca tgtaagcagc gtacaattca gaataatctt gtggttattt 15300 gtccggactc tgattctagt gctctgagaa atatgcttca gtaatttagg gctgactgga 15360 aaaggaggtg aaaaatggat aatgtcgcat tgccaccgat acaactgagc ttaagcccag 15420 aagggatgga attcatcatg aagcatgaag gtatgagaac tcgtgcttac caagattccg 15480 caggtatctg gacgatatgt gttggggcta cgcgcgatat gaatgggtat ccagttagac 15540 agggattaac gtattctata gaggattgtc tagctctgct agatagagac acacaagact 15600 ctgttcgtgc cactcagaaa aatattaggg ttcctttgct aacccacgag tttgatgctc 15660 tcacctcttt caattttaat gtaggaggaa cagctctatc aacttctaag ctaaggaaag 15720 tgatcaacgg agatgctaag ggtgatgttt actcagagtt cttacgttgg gataagataa 15780 cagtcagagg tgtaaaacaa cagagtccgg gacttcacaa ccgaagagtt gcagaagcag 15840 acctatacac cataggtaaa tattaatgga ggtgcctttt ggctattgaa actacagcgg 15900 tagtaactac cgatttaaat cccctgtacc ctcgtgacag ggactacatc tacgaagggg 15960 cggctcaact ccgcctcatt aaacaagtgt tgattaatac attccctaat atcactgaac 16020 cagtgaacct gtcctctgaa tcctttaagg ctttgtcaga gaagatcaca tttactggcg 16080 atgcaatgga tgttggtaat ctgatgatta agaacctaac tccgggtaca ggtgataaag 16140 atgctgtaac caaaggtcag atggaagctt tcatgttgaa ttggatgcag aataaacttt 16200 atcgtattgg tgcttactat atcactgaag aagatatcaa ccccggtgat tctagctctc 16260 tcggctttgg ctcttgggct aaagttactg gtgttgttat gggtactggt gttgtcaacc 16320 ccgatggatc tgttcctaac gcccagcgtg ttgagttcca agcaggcggt acaggtggtc 16380 gtgtgttcaa tacagttcgt gctgataaca tgcctctgac gacaatcaac ggttctagtt 16440 tccaagtaag ttcttactct cacgcacacg gtattacgat gggtcgtggt gatgctagtg 16500 gtcacaatag caaccctaac tggtatagtc cgggtggtgg ttatactcag acaactgata 16560 caaatactca ctcacactca atctctggtt ctgttagttt tggttatgat gatatctctc 16620 gtcagcctat taacacttta ccaccgttcc gagcagcaca catttggaga cgtattagct 16680 aaggaggtag catggctctc tatccgatta aatcattagg cgcagttggg gttatcgctg 16740 accaagcacc tactgactta gcaccaaatg ctttcactaa cgctattaat gctcggtttg 16800 ttgagcagag agtctttaag acggggggta atgcccctct ttcttatgta gacgaagata 16860 aagatctgac tcctctctcg ttcctttcaa tgcctttcga ttattatagc gcaggtacta 16920 gcttccttgt ggtaggtaca gataagaaac tgtataaact taccgatgaa agtttaactg 16980 atatcagccg taaggttgct acagctacga agaaagcatc agcgacaatt aagatttatc 17040 cagtggtctc tagtatttcc cctaaagaga cttctgtatc catgaacttt aaccagacca 17100 agattcttga agttgaagtt aaaccagtag atgcacagaa tgctaaccta gtttgggaag 17160 tgagtaactc ttcttatggt agtatcgagg ttgaccctac tgattctaag aaagctacac 17220 tgacttctaa atcagttgaa ggtaatctag ttgttacggt cagtacagct gacgattcta 17280 tctcagctca gattgcagtt aacatcatcg atggtgattc tggtatttat ctgagtcaag 17340 atacactgac tatgcgtaaa ggtggtactt ctactcttac agctatcaca ggtaaacctt 17400 ctgtaacttg gacttcttct aacccatctt tgatttcggt ttctcctaat gctaacacta 17460 agacggttgt tcttacagct cttggagaag gacaggcaac tatcaccgct gacaatggga 17520 caaagactgc atcttgtgta gtaacagcga ttcctcagat tgatagtatc tcattaagcc 17580 agacagatgt tcaaatgagt cgtggtactc agtatatctt gacagcaact gttagcccag 17640 ctaatgctcc taaccaagca atcacttgga cttcttctaa taccaatatt gctacagtaa 17700 gtggtacagg tacagaagct accatcactg gtttgcttgc aggttatacg gagattacag 17760 cagtgacagc agagggaagt cgttcagcag tttgtactgt tcgagttgac ctatcctctc 17820 gtttggcaag tagggctatg tcagcaatgg ctatcactac agaagctcct gtagaagaac 17880 cggttgttga gtcagaagaa gttacttatt ttgacactga tacaattggt attgatacgt 17940 cagggatgtc agaagggaat aacttctacg actactccaa cgttatggac ttggaaggat 18000 ttggtagagc agcaatgctt gctaatgatc ctcctctcac tggagttact cttgatgtta 18060 ttgatgcttc tcttgatgta ggtgaagaaa ttgttatcac agctacagca tctcctactg 18120 gaagttacac ttacaagtgg acggttgata agagtggtta tgtttctact acctctacaa 18180 ctaaccctac tttgaaactt actgctattc gtaagggtga gattaaggtg acttgtactg 18240 taagccagat ggtccagaaa gactatgatg cgtttgaaga ttatccttgg tatcatgcga 18300 tcatctctaa ctgtgcagta gcaactactc actacgaaac tcctcaggtt aaggaattcg 18360 attctgaata cttcactgat cttccgggat ggggtgaaca gacagttgtt gatgcagatg 18420 gcaacccttc tgttaagaag tataactgga agtgtgaacg agtaagggct tttaataata 18480 ggttgtttgc tctgaatatg agagagtcta acgcttctgg tgttactact cattatcctc 18540 ttcgtttacg ttggtctaac tttgcagagg aaaacaaggc tcctgagctt tgggatgatt 18600 atgcatacga ccgagcagta acttctgacc tagcagctaa catcgtagga cagacagaag 18660 ctcttgagaa tggttacgca gggtatattg acttggctga ctctaacggt agtttgttag 18720 atgttcttcc tctgaaagat tacctgttcg tgtatactga gtttgaaacc tacattggtt 18780 ctcctactaa caacacctat cagcctttga tgtttaagaa gctgtttaac gattcaggta 18840 tccttgctcc tgaatgtgtg gtggaagttg agggtggtca ctttgtagta actcagaacg 18900 acgtcattct acataatggt gcttctaaga aatctattgc ttctaaccga gtgaagaaca 18960 tgctcattaa tgagatttgt ctggtgaacc caattgctac acgagttcat cttcatcagg 19020 ataagaaaga agtgtgggtt ctttatgtag gtccgggtga acctaaagaa agttttgctt 19080 gtactaaggc agctgtatgg aactacgagt ttgatacttg gtctttccgt acgattcctt 19140 actcttactg tatcggtctg gttgatcctc ctgttctgga acgtggtcct atctgggctg 19200 atttccaaga gatcacttgg gatgaccctt cgattgacaa actggtgtgg agaaaagatg 19260 caactaactt ccgtcagcga gttacaatca taggttcttt cttgaaagga ttttaccaag 19320 ttgatatagg agctttggat tatatctatg acagaacaaa tgatgtggtt atagagaagc 19380 ctctggaaat gagactagag agaacgggga ttgattttga taacgtcact aatgaatgga 19440 atcagaaaca cattaaccga tttagacctc aagtaacagg aacagggact tatacttttg 19500 aagcaggtgg tagtcaattc tctaatgagt atggacacaa ccactctact aaagagttta 19560 gggttggtgt agatcgtcac gtagcagtca gactgaacca tccataccta ttctataatg 19620 ttatagataa tgatgttaac agtaatgcgt ctatgaatgg tttaaccata gagtttgctg 19680 ttggcggtcg aagataacaa ctagggagct tcggctccct tttattgttt ataattggag 19740 gtatttatgg gtgctggagg ttttcgtaag aatactggaa gaaacaacac cacacttccc 19800 tacaacgttg ggtttctaaa caatgtagag gatacggata aatataacgt tgcagtttat 19860 gatgaattac agaaagtcag tacagcaacc aatcaaatgt tccaagcaat cgacgagatt 19920 catgatgaga tagatgttcg tattaaagct cttcatgcaa tgaatctaca gttcgatgaa 19980 cttgagaacc gaattactac agagattgaa acagcaattg ctgatatcca aacacagatg 20040 ggtaatctat ccactgaaga tatttgggat agaagcacta atcctcctac taaattggat 20100 ggtacagttg caggtttcaa aacttctatt gaaggtaatg acttaaagat tcagacagta 20160 caagggattg tgaacgaaca gggagaggag attgctttag ttcaaacaga acttactgct 20220 cagggtactc agattgctga agtggatggt aaggttacta ccgtacagaa ctctctttct 20280 caatacatga gattagacag ctatgaagct acatggggtg tcaactcttc tgttaacggt 20340 cagtatgctg gagttaaact tactaacaac ggtacgaata gtcaattcca agtaacagct 20400 aacaagttta ttgtcggtga tggtaactca ggtaacacac ctttcgtatt tgaaggcggt 20460 agggctagga tggagttcgc tgatattaag aacgtcaaca ttaccacagc tcagattgct 20520 aatgctcgta ttcagtttgc tcagattgat aacgtgtgga ttactgacgg tcagattgct 20580 aaccttactg ctaacaagat tactgctggt tctatgagtg gttctaattg gagacttaca 20640 gttggtggag attttgttat gggtggtaca ggtggggcac agctgtggat gaatggtaac 20700 aggattgatt tctacgatgg tggtggtgct ttgagaatta gaatagggag ttggtaatat 20760 ggcgtatgga atatggtgtc aaccagtggg gccgggtcca gctactccgg ctgctttgtt 20820 tatagatagt aatttaacat ttccacaata tctaggggct tttagtatat ccccttcttg 20880 gagtgcaagg aatgcctctt ttactgtcgg ttcctttgac ggacaaggtg agttgcttat 20940 tgttcctact agtcggttga ttcaagatta ttatgttggt acagacctga tgccaagctt 21000 cagcactgtc actggcatag gaacctctgg tcagactgtt aatataaata tagacccagc 21060 tggtgcgagt ggtaacaaca accgtaactt ccccatgagc tttcaagcct ataaactctg 21120 gcctagaggt aataggagtt atggtatcac tttctctaac tcagctgact tcttctctat 21180 ctctgatagt ggagttgttg gtcaatgcac ttgggcatac agaggtggtg ttggtgacgg 21240 cttcactgtt cctaatgctg gacctaatgc tctggttttt gccaattgga gtcagggagg 21300 tgtgtgtgtt ggttataatc caagtaatag tcaaatgatt attactcaca atagatctaa 21360 tcaatctggt aataacagag gggcttctgt tggagacatg cgaatagcag tatttgccag 21420 tggtgtagga gttcctgagc acaatggtgg cctaaacatc tattcaccgg ggactacttc 21480 ttgtgtcttt tctacctaca gaactccttt tgccataaac agattcttcc cttctagtgg 21540 aggaggtact ggattaggta ttcctatgat gtgccttaat actactaatg gagctataac 21600 tggtcaaggt gggggttgga tgtgggttca tgaaagaaca catacaatgg agaatggtac 21660 tgttggtaca ggttttggac ctctggttgc taactggaca gatcaatatc caatcaatgt 21720 aaatataaat ttgaacttca catacccaat gctagaaggt tctttaatat acgcttaagg 21780 aggatttatg acaatacgca tgggagtgtc cgagctagac cctatggtcc agcaagcttc 21840 taaaaagtac ggcattcctg ctgctcttat ccatgcagtt attcagaggg agtctagtgg 21900 tagaccttct gttgcatcag gtacagggcc ggtaggttta atgcaaataa gtaaagggct 21960 ggctaaggat tatggctaca aactagaaga tcgtctagac cctgctaaga atattgatat 22020 gggtgctagg tatatcagag acaacttaaa agcttttgat ggtgatgtga agaaagctat 22080 ggtgggatat tctgaaggga ctggtggagc aaaacaaatg ttcgctggta agaaaggatt 22140 cacacctcag gctttggatt ctatgaacaa taagaacttt cttcctttct atacagctaa 22200 tgatgctgct ggtcaggttg ctaacccgat gggtgcttta cagtcagtac agccagtgaa 22260 tcagttctct cctgaattgg ttactagaga aggtgctgct aatgctccta ctcaggctga 22320 gattgctgct gctaagacag gtgctcttgg tggctcccct actgagttct attctgatga 22380 tgtagaggaa gctggagaag atcagaagcc acaacagaac tttggtaatg ctgcactagc 22440 tgctgctgca atgttagcgg gtaaggctcc taagtctcaa acagttcaga gagcacatgc 22500 accaactcaa gcaggttctc aatacagccc acatacacaa gccgttctta gacgtttggc 22560 aagtgttggt gttattaaat aataaggagg acgtatgtct ttattcgggt ctgcttctaa 22620 gactaaaact acagtggctc catcttctca ggttgatgct ctgttaaaac agattgtagc 22680 taatgctcag aatatgaata gcaatgatgc aggtttcatc tctcagcaat tggctggtct 22740 caatcctaat caacaagctg ctctttctaa cctagctaac tcctctaccc tgaaccagtt 22800 gagtggtatg tatactcctc gtacacagca gggcttagat cagatgaata ctctggctca 22860 gaagtattct gctctaggta acaactctat tggtgctggt gatgttaata acttcagttc 22920 tggacttaat aactcagctc ttgctcagtc aactggtaaa gcagcttcta atgtatctct 22980 cggtaatggc tctgactcag gttctctccg tcgtgcttct gctcaaggtg ctgctttgaa 23040 ctctgctaat actaatctgt ctcgtagcat ctctggcaag aatttgggtg ttaatgcgct 23100 ccagtctaat aactctttcc aacaaggatt gcttaatgct cagactggat tagctggaca 23160 gaatttgaat cttggtgctc aaggtacaca agctgctcaa caggctattc agaaccaact 23220 tactgctggt aatctacagc aacagcaaga tcaagcacaa gctgacctta attggcagaa 23280 cgctattggt agtcaacaat atgggtggaa tcaactcaac aaccaactga atgtattaaa 23340 ctcagtaagt cctatggctg gttacacaat tactaacaaa ggagccgctc ctaatacaac 23400 tcaacagctt cttggagctg gtgtcactgg tctaggtatt gcaggtcggt taggtgcatt 23460 cactcctagt caacaaacta cgaatgcttg gaactcctac aatgcttctg gtggtcaatc 23520 tggtatggct ggtcctatga ttggtgggtc taacctttcc aactcttctg gtcagagtaa 23580 ctggttgtct acagctggga gtaatatttt aggtggtgtt ctcggagcct tcggggcgaa 23640 ctaaggagga gttatgggtt tatttggtgg agggggtagc acgtctactt ccaaaatgac 23700 aagacctgaa tatattcaaa attatattga ccaactagtc aatcaggtga ataacacaag 23760 ttcaggcgat tatgtttata aagacaatgt aggttttaat cagaaccaaa ctgaggctct 23820 gaataacctt tctcaatcag gggcactagg tgctctctct tctcagtaca tgggtgctgc 23880 tcaatctggt ctaggttatc ttgaccaagc taatactggt tacaacaatc tgactaattc 23940 aggtcaaatc actggagaac agattggtgc tttggctggt caactctatg atgatgacgc 24000 tgttcaggct gctattacag ctaataacga acagacccag caagacttgg ctcgtaatgc 24060 tcttcctcaa ttggctcaac agtatgctgg tcagcaaggt tcaggtgcac gtatggctaa 24120 atcttttgct cagggtgatg ctctgaatca gatgcagaat acagcaacca atattactaa 24180 cagtgcttat gactctgctg tcaatcaggc tcagagtatc ctgtctggta atagacaatc 24240 tcaggcttcc gctctaagtg gtctctctgg tatcggtaac actatgtcta accttggtac 24300 tcaggcaggt caactttctc aacagcagat gcagaatcaa tgggcagcag gtttgcaaca 24360 gcaacaacag caacagtctg aattggacaa tgcttatact aatgctcaga atgcagccaa 24420 ttggggctat caagacatta ataaccaact cggtgcagca ggcgtactca atggtgcttt 24480 agggacaacg actactaata aagtttcagg tggtggaagt ggtttcttag gtggtgctat 24540 gagtggcgca gcagcaggct cttcctttgg accttggggt gcgttagcag gtggggtcat 24600 tggtgggttg gctagttctt aattaggaga ttaattatgc aacaaacatc ttacgcgtat 24660 cttcgtagtc aagacccgcg caatcagatt tatggtgatc gtcagagagg ggttacggcc 24720 tctccttaca tgtctagacc tgctggtcca gctgctgacc tttctatgta tcagcgtcct 24780 gctgtacctg ctggtttgga tgaacaagta caacaagctc aggctgatca acaggcaatg 24840 gtcaatccgg gattcaagca acgtttgtat gaacagcaag gcatttctgt ccctaccagt 24900 ttggctggta ttgacgctgg tattaatccg ggtgaagaag gctctatgat acctcctgat 24960 gtagctcctg cttctagtcc tcttccggtt actgctcctg ctaaaccaat tgaagagtca 25020 gataaagaat ttgcccctga acaagtacaa caggcacaag gtgtgatgaa tactatcgct 25080 tctggagttc ctcagggtga aggtatgagc ggtgctactc gtgctgctga ccaagaacgt 25140 gttaaccaag atatccttaa agttgcacaa tctaagaacc cagcagaagc ttggaagact 25200 atgcagaaac aacctttcta tcagaactct agtttctata ctggtttgat gggtgtaggt 25260 ctttctatta tgtctggtaa gtctccgatt gaagcattcc agattggttc tggtatggca 25320 tctcaggatg aagctaaaca acagttggag ttgaaccgtg ataacctgat tgaacaaggt 25380 tactctcaag attctgtagc tgctgctata gcttctggtg atccttcgat gttgaagatg 25440 cgtcagatta gtcctcagga gaagatggct cttgctgctc aggatgaagc tcgtgctaat 25500 caagagtggg atagacgtca acagattcaa gaccagtcgg ttcgtgaacg tgctgctgaa 25560 actcgtcgtg ctgctgaaga acgtgccgat gcacagtttg ctaaacagaa agagcttatt 25620 ggctatcgtg atcaggttaa agcagaccgt gcagaacaag ctgctcagag cttcaacttc 25680 agtacgaaag agcttcgtaa cgttcagaat actgctgaag gctctgttgc taaacagtgg 25740 gcagaaaaag cagctctgtt taaacagtct cacaaagact tggatttggc agacaaagcc 25800 attaaagatg gtgattatca atctgctcgt accgcttaca tgcaggctat catgaactcc 25860 gcaagggctg aaattggtgc taaccgctct ctgcaagcag atgacttatc ccactttgca 25920 gaagacccgt caatctttgt taagactggt aacacttggt ctctcaaagc tggttatcgt 25980 ccttctgatg cagctctgtc ttatgctcgt aagcaggcaa cagttggtgc ttcttccgct 26040 gataagggtg ttctggaatc taagaaagct acaattgatg cttatattgg ttctggcatg 26100 gataagaagc gtgcaactgc tgtggttaac cgtggtatcc cttctggtgg tttctatgac 26160 ccatacaaga cttttgatgc agaagctgat gaagctgttg gtggtgcagc aactaatccc 26220 aaacttaatg gtgctctgtc ttctaatgtt gattggatga ttcagtaaga cctaagtcct 26280 atctcttata atgtatatag gaggtaggac atttttattt ataaggagaa attatggcta 26340 acttaaaacc gtgggaagaa gcttctaaaa gccctgcgta tttgaacgcc tcccctgagc 26400 aacagtctca gatgcgagaa caatataaag cagcaggtgg aacaattcct gaggcggctc 26460 cagctccgaa acaagaagaa caaccgggat taattggtga ctttgctcaa ggtgtgtcca 26520 atgctattca atcaggtggt gctaatattg cagctagtgc tgctagaggt attggtggat 26580 tagttgagat tggagagaat ctaactggtt cacagacaag ctatggtaag cgtattcaag 26640 agaactctaa gaatttatta gaagaacgtc tctctaagat tgaagatggt attgggaagt 26700 cagctggtca gtatgctggt gtcgctgctg atatcggttt gacagttgct aaccctgcta 26760 tagctgctgc tgttattgca gctcgtgaga caggccgtgc ttatgcagac caaactccag 26820 aagagggtga agagaagtct gttcttgatg ctggtttagt tggtggtgct aactttgcag 26880 ctcagaagat tcttccgggg atgacaggca ctgcttctac tactctaggt cgtgttggtc 26940 agagtgtagc atctaatgca gtggctggtg ctaaaggtgg tgctcttgta ggtgctgctg 27000 aagcacagaa caaatatggt gatgacacta ctcttgctaa tattattgaa ggtgctgtag 27060 aagaaggtgc gatgggcgct gcttatggtg gggctattgg tggggttcat ggattgatca 27120 gtcgtcctag tcctcaagta tttgaccgta ctccagagca tgttaaaact gatattgccg 27180 ctgaagatga agcgatacgt agtgctacta atgctgatga attacgtgaa gcgtattcta 27240 aagctgacaa tgctaacact gcttctgccc ttaaccttct ggacgagaat ggtttccgtg 27300 ttactgatgc tgtggctatg gattctcctg ctgctcagcg tatcctgaac actgaccaaa 27360 gtcgtgctga gaaggctgct accattctgg aagatcgttc taacgttccg ggtttgaacc 27420 ctcttgctgc tcctaacaag cgtcaaggta aagcgaacgc tgataacgaa cacaaccaga 27480 aacaagccgt agcaatgaaa gatgcgatgg ctgactatac gaaagacaac actaagtctc 27540 tatctgacgt actggacaat cttgaccgtg aattaacatc tgctcgtgct agtggtgact 27600 ttactggtag tgctatggat attaagaatc gtactcaagc tgaccgtgac ttcattgatg 27660 cttacaaaac tttctataac gaagctaacc gattcaaagc tcgtgatggt gaagacttca 27720 acaacttcat cgagaaagca actgaactac agaagctagc tgataatgtt tctccagaga 27780 tgaagactgc tattggtggt ttgaagaaac tcaaaggtat gcctgaaggt ttcaacccaa 27840 tccaagatgc tttcactctg aataacacag ctaagttaat gagtaaccaa gaccgtggtt 27900 ggacctctct aactgctgac ggtttcaaag aagctgctcc tccggttctg attaagaatc 27960 cacttggtgc tgctaaacat gttctcaacc gtttccgttc tgaccgtgct cgctctcaac 28020 gtgaaggcca acaggcggct aacagcgaag ctattcgttc tcttgctcgt agtgatatgg 28080 aagtaactcg tgctcgtgct caggctgatg aagctcgtgc tgcgatggag aaagaacctg 28140 aagttgatga cattaaattt agaactcaaa atgacccgga aatcgaaccg gttcgggagc 28200 cttctgctac tactgtgacg gaagaaattt cctctcctca aacctctcct ttaactgctc 28260 gtgatttggt cgcacaaaga cgtgctttga aggaagctca agctagacgt caggaagccg 28320 aagctagtaa gattcaggaa gagccaactc ccgagccaga gccacaagaa ccagttacta 28380 cagcgagaga tttagttgct caacgtcgtg ctgtttcaga agctcgtaaa cgtcaagaag 28440 cagaagaggc cagcaaggct gctgagagcg cccccgagga accaactgtt caagcagagc 28500 cattgcctac ccgtgctcct aaagctcctg aggttgaggc tgagcctgtt aaaccggcta 28560 ctggaatcga acgtctggct gctatgtcaa gtgctgctcg tcaagctact aaagaccgtg 28620 cagatcgctt tgctcgttct ctgtatacgg caggtgcgaa ggctaaagct acagctgaaa 28680 acttcctttc ttataaaggt gacccgaaag agttgatgcg tcgtattcgt caggaagacc 28740 aagcgaacaa ctccgaacgt catgctgaga tggctcgtaa ccaacttgct tctcaacagg 28800 gtattgctgc tgctaagtca caacgtgttc gttcttcgtt tgctgattgg gttaaggaac 28860 gtggtctgcc ttctgatatt gctaccaagg cattacgtgc tgaggagaaa ggtattggtg 28920 ggaacgtgac tagtttggat tcactgaagc gtaggtctga acgtctgtat cagaaacaac 28980 gtgatgatga atttaacaaa ctatatgagg aagctcttgc agagaataag aagattgaca 29040 aagctgatgc ccctaaactg gttgaccaga agaaagagtt acacagtgaa atcaatacac 29100 ttcttgatgc agagccatta cgtccagctc agaaagatgc tatgcgtaag atgatggaag 29160 attttgtaga tgggaagttt aaagcttcag agagggctgg aagggaagat gctttggaag 29220 tgggacagat gaaggatata tgggatggct ttatcaacac ctataaccgt gaagttaaca 29280 tgttcaacaa agctaataag aactctcagt acgaagcagc agctaaacac ctgcaagcac 29340 gtcaggaacg tcttgatacg ctccgtaaca gggctaaggc tcgtagtgat gccaaggcag 29400 cggtagagac agaaaggcag tctctcgaag ctatagcggc tcagaaatcg gagatggaga 29460 aactgatgaa agatctgcca gagtcagtac gtgaagctca ggaaggtcgt acgctgaaac 29520 agttagcagc tcaccatgag aagaactctc ctgtaccacc agagcgtttc cgtagtatca 29580 ttgaacgtat ccacaacgca gagagtgatt acatcgaccg taaacgtcga ctcactgaag 29640 cggaagaaga tgctcgtgaa gctaagtgga ataagatgta tgagcaagcg gaagagatga 29700 accgtaagtt cgatattgac tctcgtgtac agcgtgataa gaagctcaaa gaagctcagg 29760 aagcagagaa tgaccgtatg gcagtacaac gtcaacgtga tgacattcac aagcgcctga 29820 tgcgttctat ggaggataag ggtattccta aggaagatgc agaaatcttt gcaggttctt 29880 atatggataa ccgttactct cttctggaga aacctatgac tcctactgag caccagaatg 29940 ctcgctctcg tattgaatct gatatctcta agtttgctaa ggattttgag aagatgacac 30000 caatcgagaa agatattgct aaggcaactg gtgatatgga tgggaaggta gaggtggatg 30060 cagacacttc gaaagctatc gaagatgcga agaaggtaga ttctgagttg aagaaacttc 30120 aggatgaaga gttggaaatg gagaaagttc gttcggcgtt gccggaagat gaaagatctc 30180 aagcctcctc tgatatactg gaaagtatta agaagcagga agatgacttt gctaacaaag 30240 cagagaaagc ttttatcgaa gggaaagact tgcaggagct ggctagtgta gcggaggttc 30300 tagatagggt tcatggtgct gacaaagctg gtaataaccg tcgctttgtg cgtgctttac 30360 aaacggctgc ggagaacaag aaacaatatg gagataatcc agtggcttgg tttagtgctg 30420 atgattactc tgctattgcg aaacttggtg ctgcttcagc gggtggtaac aaatctagag 30480 cactggagaa aatctttggt tctactgctg aacaagctaa agggaaactt ttagggcggg 30540 atgatgtagc gaagattaga catgtgattg aagagaatcc tgatgttaag attccttctt 30600 acaagacaac tactcgacaa gagtatttac agttcttgga gaagtataat gacgagggag 30660 agttgaaagt caaacgtggt tctctgtctg acaagttgga gagaggtaag cgtcaagcta 30720 aactccgtgt tagagttcga gctaaggagt aaacaagaaa agcccccaac ctgtaaaaag 30780 gaagggggct tctttatttc tagatataga ttcgacattt tgatatcaaa cctttatcac 30840 ttctcttgcc atatctacta tacagtaggg gtgtgtcctt gctcttaata gaaaccatta 30900 gaacaaagag aaaacccaag gctattagaa tccatcgaat tcatcataca tcccaatttt 30960 cttcttcgaa gtcaatcata agacctccta gattagatca taatacttaa taatatcaga 31020 gagcttaaac tctcgaatgt cattcttgtc attgtcccaa gcggtcatta tccaacaagg 31080 tttgccccca ttatgataac gggaagaagc aactgaggtt cgtataggaa caacggagcg 31140 gtctgcaatt tcacctctcc aattacgata gctaaagcga agagtttgtg tcgtagatac 31200 ttgatcattt gtattttcct tgttcataga ttcaaagact tccttctctt caggagtctc 31260 tagatttttt aaattcgcgt cttgcatcat tctcttcctt agttttaatc ttatggcaag 31320 gttctttaca caagacttga aagccatcta cctcaaccaa agctctctca atgatttcat 31380 cccaagatgc ttcatggtcc actggaacaa taggattaat gtggtcaaca gctacttcct 31440 taagaggaga gtcattacca cacatggcac acttgtagtg cattgccagc ttgccagtct 31500 tagggttgat cttacgttct gtctgagctt ctttgagagc atcaaacata ggcttccact 31560 ttctagtcag agaacgaatc cctgacttga tgaaggaatt ataacgtgct tcagtccact 31620 ggtttgaaca tcttgttttc tcagccttcg ccataaccta catccatatc agcgtcttcc 31680 caaatacgtt tgttaatacg atgtgattct ttttcagcac gagggatacg tccagtacga 31740 cgatcaaatg cttgatgttt aatcttctgg tctttacgag actcacgatt agaggaattt 31800 tcatcatacg aacgatagtc aaatttaccc attataatat ccttattacc aagttttatc 31860 taaccatgac cattcaaaga ggtcaccttc agcttgtccg acgaagagca atcgagcatt 31920 ttctagcatc ttagcttctg cttcttcctc gaacctctta tagtattcca ttcgtataat 31980 gtgataaaga gtacacttat cgaatacctt ctcaataagt ttgattgcag ccttgctacc 32040 aataccttta cgacgcataa ctaggttgcc accccacatc atttcaactt tctcaccaca 32100 tccggggatg ttgtctgtac tgtccccaat caacatctga tagaagaagt tctgccatgc 32160 tacttcctct gatacataat gaggaggtac ttctttctgt cgttcaccac aagcccagcg 32220 ataatgccat ccgggagtga ttaacaaatc cttatcacga gtacagatga tagtgttctt 32280 ctcaatacgt tgtcgttcag cgagtgcatc atctgcttct ctaccaaaca cctcaacgta 32340 ctgataatgt tctttgatgt aatcactaac gttctgccaa tgatgtggcc tttctcccgg 32400 aggtcggtta gctttgtaac cttgttgctg agcaatatca aatcggaagt taccaccttc 32460 actaaaatag aagacagttc tttcacaacc agctcttgag atgattacat caatcttatg 32520 ttggagacgt tcaatcaaga atccataatc aactggaaca cgacctgtgt ctagaaaagg 32580 atgctcttgt gtaagagcac caacctcata ccgtagaaca tctccgtcca caatcgctac 32640 ttctggataa gatatatcac tcatgatttc ctccatggtg tcggataagg tgacaattag 32700 cacagagcat gacatattta tctaactctg ccttaagttt aggccagtac cgatagtatg 32760 aagggttttc gtcttttgtt tgttcatcca gatgatggaa ctgcataaca caaggtgggt 32820 attctttttt acaatctaag caaacacctc ccatgtattc tattgctttt atcttaagat 32880 ctctatttgc cttattagca acctcttgcc gtctttttct attggcagca taccaagctc 32940 ttttcttttc tgcggtacaa atcttacaag ttccagtcac acccttagtg cacaccttat 33000 tggggtgata ttctgttagg gctttttcaa ccccacaaac gttacacacc ttagtatctg 33060 catccacaat tgctacttca ggatagatta catcaggcat agagatctct ccgtgtctgt 33120 gcaatactgt actctaaata ctccactgcc tcttctacag aaccatcatt aaagagaaga 33180 taggttgaaa ggttcgtgcc ttcaatatag gaacgagagt ctccttcaaa agtacatcca 33240 tcccgataaa tctgaattac tcgaacatcg aaatgctttt ccagttcttc aacttctgct 33300 ttgaatccac catcagagaa aacgataaca tcgttctgtc ctagcagttg ttgttgttga 33360 atgctgctaa cagcaagttt acccatgcag gtatccccaa acattggctt cagatagttc 33420 tcactagttg aaatcatcaa atctcgaact gacttaccgc cccattcagg acggttatgt 33480 tctttccact cacggtcgtt gtacagctcc atgaactcat gaagagggag acctaatgca 33540 gcagcagcca ctcgatacat aggctcctta aaagccttta catgaggtct aaactcatcg 33600 ttaccgagtg tcttctctaa ccaagaatta gcgattgtat ctttaccaca acccggtggt 33660 ccattcagaa ttactactaa tggtttactc attattgaat catcccttga agatcttctt 33720 tatcaaagtg cttctctact accgcttcat acagatcacg attctctgca taacggcgat 33780 agaaagttcc gtgtgaaatt cccagctcct gctggatgtg atcatgaggt attccaagga 33840 agagcataga gaacatgctt gccattttct tggtatccat aatatcactt aagtcttgga 33900 agaagaaaaa gattctagat tccaagaatg ggtctccagt agagtcatgc ttatccatcg 33960 caatctctaa aggactttcc cacgagttca tctcaggagg tctaaacata tcagcctctg 34020 gaatagagta gatacgtcgg tctccaactg tctgacggtt agaacgaatt gaccagatga 34080 tattcttaac acgataagtc atgtgctcaa tatctttttc atcagtgagc agaagttctt 34140 cttgctttgc aaacacaaaa cgccaagcgt tcagtaacac ttcctgagca acatcctctg 34200 cttcgtgata atctttaatg tcatacataa aatacttatg cacaactttc cagttctccc 34260 gatagaattc atcgaaggtt ggataatact taataggaat gccatgaata cgatagcgtg 34320 taggtgttgt ctttttcttc ataagtcgtc cttaacttac tttacattaa actacgcaga 34380 ataaatacat agccgtttca gggcaaggga tggctgtctg aatgccactc actttaatcc 34440 acatatcctc ttcagagtca taataggccc aataggattg atctccatct acaaagaact 34500 taaaggttcg tccccaaatc tggttgttat actcccatac tgcatgaaat tgagtaacac 34560 cttctggtat tgtaagttcc gctctgatca ttgtatccct ctcttcttga gagctttcag 34620 taaaccatta tggttagcag cacaggttgt gtagttgtcc tgataccact ctaatgttgt 34680 tgcaaatgcc actccctgtg cactctgaag caaagggaga tcaacatcac attcagcttt 34740 taggtttgtt ggtaagggca ctagctgcct gctgttgtcg ctcgttgaac aagctgacaa 34800 attcatcgct agcacacaca ttgtaaaaaa ctggcttagt cttttcataa tgcatctctt 34860 tgtaaatagt ttctgtgttc ttcttccagt ttgataaacc agttagaact ttctgagcta 34920 cttcatcagc agccttagct ctctctatct ctatctcttg ctgtttcttc tcttggtcaa 34980 gagcattact atcatttcga tattgctcaa acttcatacc accaaagaaa accagagctg 35040 taaagattaa gaagccgata ttattttgaa gcttttccat tttaactcca acgaaaaaag 35100 ccacccgaag gtggctaagc tattacaagg agagattcat tagaaagggc tgtcatcttc 35160 ttcaaaggta gattgcggct cttcattaat agtagggata cttggagtcg cgtctttctg 35220 ttcagtgtca gaagcgcgag taacttcacg accggccaga ggaatgtttt tagcatcgtt 35280 agcttgagtc agaaggttac gttggaatgg aaacatagct tgataagcag ccaagttggc 35340 gtcagtttca gcgtacggtt cgaagaagat aaggtcgcta cgaggagcac ctactgcatc 35400 acgatacttg gtcgggattg gagtgatgct cttgatggag tttttcttct caccagcttt 35460 gttggtgtaa gagttaacta gaatgttgac aggctgacca agcagcatat ctttgaacca 35520 acccaaatca cctttcagtg cttgaacact tgggtcgatc attttaatta ggttcagaat 35580 accagagttc ttagctcgtg ggtttacatc gaaggattta aactgacaag caggacgtgg 35640 gtccaatgcc ttaccatcac tatctgtacc agttgcgtca acaccaatca gttcaaatgc 35700 caagtccata cggaaagctg ggttcttagg agtaactgct ttggtcttag ggtcaatcca 35760 agggtcacga ttgtgaacac ctaaacctac tacacgaaca attcgtgctt cgtactcgcc 35820 atcagggatt acatcgtagt tggatttacg ggatacagac agttcaggga tcatagacat 35880 attaatattt ccttatataa atttaagatt gttgctctct ttgagcggtt ggtatactat 35940 atacattaca cctatacaga taaggttcta ttatactata acctttttgg tagtagataa 36000 cagtaagtag taatgtatat atggtatagt tagtaaaact attgcacggg accgacgact 36060 ataaggcgcg gacaaccacc tctcataagt ttgttagaaa ttgagagact accggaaggg 36120 caggagacac ctgcgagata aaaatagaga attaaggaaa tccttgattt aggttgagga 36180 ctcctaggac tttgattctc tattaaaatc aaaagaaaac taattaactt accattcaat 36240 aaactgattt agattagtat acttaacttc tctctgaaac tgaagattag attctctctg 36300 tttagtaaga agattaagtt catcttcctt tcttttatca aactcttttc taatctcttg 36360 cagagacaca gctctgtaat caatttgttc catagaagta caaaagtatc tccaatcttt 36420 gatagtagag ttatgagtat gagcatagat attatacttt cctctcattt catcagagtg 36480 aataggaata tgagttaccc aaaactcttt gtacttataa tcaccactga tgtgtctaaa 36540 gactttcatc atatcaggga gaagttctaa tctgtcatga tttcctaaga caagatgttt 36600 ctctcctttt acttcagcga tgatatcaag ggcatacttt tcaaacaccc aatcaccggt 36660 aaagaaagtt ttatcacgtt tagtaacatg gtcaaacgct gctaagatag ctttgtcgtg 36720 ttcttcaata ctactaaacc tcttcctaaa gctacagatg ttacgatggc ctaggtgacc 36780 atcacctgca aaataaactt cagccattct cgtatgtgtc ctcataatat gagtagttga 36840 aggattcagc tttcgtttca gcctcctcca tatcgtctgt ttccagaatc actctccagc 36900 cagaaccgtt aggatattca gctccctcat ctagccagac tttgtatcgt ttagattgtg 36960 caaccatcgc agatgtaatc tccactaaga gactcttgta ggagtcgaga ttgctcatcg 37020 gcatacagag catcaatttc ttccttgagc tttgcaagtt ctacgaagtt tatcaattga 37080 ccataatccg tcgcatcagt gacgaattga aacaaacctt taccttcagc tttaacaata 37140 tactctttag acattataag atctcccttt gtttgctgtt aatgaccgtc atcgctgtga 37200 ctggattgtt tgttccaaga tattccagaa cagggacacc cttagtcggt ttatcagcct 37260 ctgtgtaatt cttaaccttg ccaccaagaa ccgctagaga agcctgagct tccgcttcat 37320 catagtgaat tcggtcaact gttttctttc caatcctata agtgtgaacg taaatcattt 37380 aacttcctca atatcaacta attcaaaact tcctatttct tcaagcttaa taatctcttt 37440 aaccttctca acagctttaa tagggttttc ttcttctaca actttacacc ctgatacttc 37500 aagactgcct gttccacgaa ggtataaatt caacttataa actactttgt agtatttcat 37560 cgtgctccca atttctttca taatatccag agttgtagtt tagggcgcat ttctcggcct 37620 cttctatgtc atctgtgttt aggatattaa cccaaccacc aacttcatca aaaccctctt 37680 cttcttcatt aaaccatacg ctgtatttct tcatcttgat aagactctcc cccaatcctt 37740 gatgtaacag cgaccgtcaa cccactttgt atctgactta aattcttgag acagtctcac 37800 ttcacaatct tctctactgg catcccctcc gatatcaatc agatacagga atagggctat 37860 acctaaagcc caaagaatta acttcttcat cgtatcacca gaggctcttc agagtataca 37920 ggagaggcta tttctaccgg aggtgctgct ctaaggtcat taagccttac gcccttcagc 37980 ataccatcta ggtggtccat tttagggata cctacaggga agtcatcaat ccaaacatcc 38040 acaaagaaac ccaagctctg ggtgacggaa tccttctgaa caccgttgca aaagataaca 38100 ggaatatcta gacttgctgc atctgctaga agctcctcat ttgaataccc attgggttga 38160 gcaaaacgat aggtgacaat acgtacatca tgtccacgag atttcataag acctacaatg 38220 tgggtccaca tctctttgtc cactgtaaaa gtatcatcat aatctaaacc aatattcata 38280 ttagcctctt agacaagaaa agccacccga aggtagctta tttattttgt gtttccagct 38340 cgataatacg aaccttcaat ccattgattt ctgcatcagc agcttctaac ttaatcaaac 38400 tttcttcagc gttagcttca atctggataa cagcagtagt cagttcatac aaagcttgac 38460 gtaaggtatc cttacccata gagtgaatct gtgcaaggag gtgttgttta ccttcagatg 38520 tttccagagt ttcttttaat tctttagagg ttagagtcac tattaattcc tttcgtagat 38580 tttgagaact gtaccaccat cttcttcaat ctgagcaacg acttcatcgc gttcttcatc 38640 agaataaact agttggttat gaaatttacc agtggagtct tgataataga tattccactt 38700 tggagcaggt ttacgaatca ggtccatcat ttggctttct cctctcgttc tacccatgtt 38760 tgaaataatt caccgtaatc tttggcgtct tcaaaacgcc cttcatcctg tgcgagcttc 38820 tgttgattca agcaccattc agaacttttt acttcttcca cttccttgtc tccttaaaaa 38880 gaactaatca ataagactag ttcatttcgt aataccattc aactcttcca ctgaactaat 38940 cactacttct ccaccacaaa gaggttaact tcaatgaaag tttcgtattg tgttgactcc 39000 tgcttacggg tgtagtagaa atgacgacct tctaatgcac ctccgtactg agtggcacat 39060 cttttaacca tctcttttat cacatctata actgatacgc ctttccacgg acgtaaccca 39120 tgagtaggaa tttctgctgt gcaggttact tgtgtataac ctacatcttg tagaggtgtc 39180 tcaagaatca gctttgccca agcatcaaaa ttaagcttgg ggttatattt aacttcagtt 39240 ttgatatcaa tcattagtga gtatgattcc agtttaatcc aatcttacaa tcagatgcaa 39300 gaggacattc catcttgaga tattcccctg ctttacggac acactcttca gtgagagtgc 39360 gcagaatatt aacatcatca cgatgtacag tgaactggcc ttcatccatt cagttattcc 39420 caaattactt cgggtgtcgg actatatcat aacagcatag ctaatttgct gttgttggcg 39480 cttcgagggt ttccctgtta accccctact cccttaggat agtctctaca ctttccactt 39540 ccgaatccat ctacaaccga ttccgaagct cacaccaaac ttctctgcaa gatgtacacc 39600 agagcattct gtttctagcc aatagagcct tgcagcctct tgcctatctc catttcttcc 39660 agtgtttgat tctattgcat gttctgaacc ttcaagcatt tctaagtgct caagattgga 39720 acaagctcta ttcttgcatt tgtgatttat ttcataaccg tcaggaatag aaccaaaaga 39780 ttcttcccaa acatgcctgt gatacatgac ccatttgcca tctattactt ttctgaaata 39840 accattttta ttaagtttgt gtgagatagg gatgatacac cctgtttcgg ttgtttccaa 39900 aacaagagtt ttcttagcca tatcatctcc taatagctta gctcggtatt gccctcaact 39960 taatggtagg gtttcaccga gttcaccaac ttttacatgc acaatgaaag tttatgcatg 40020 tcgataacct tcgcacaacg aacaccatca cgctcaatcc atttattgag gaatagcatt 40080 gcatacttca taacaatcgc cccagcgctc tgtaagagcg tatttaaggc tttatggacc 40140 ataacctttc cagttgcatc tcttctcatt gtaatacgac gtccatccac accgtagatg 40200 tatccacttt gtgctttctc ggtcatacgt tcaataagga ttgcaataca aggattctct 40260 gctaagaatc ttgctctcat aatagcccct tcgtcagagc caccacctac gatacttcct 40320 agtttagcgt cacctgcccc atagttaaag gcatatataa acgtcttagc gttatcgcga 40380 gtaggaagac cagcaagttt ttggttatgt gagtgaatat ctccttctaa aagaatatct 40440 ctgtacataa cagcagaagc tagaccacgt tcagcttctc gtttcttagc tgggttgttt 40500 tctcgctctg cttcagcgag catctcacga cattctttaa taaggtagtg agctagcatc 40560 ctcagctcaa gaccagagcc atcgtaacct aagaagattt cacgaccagc aggtacatag 40620 aacttatagg cacctacagg tttgtctttc ttgcccggtt ttcccacttc gtagataatg 40680 ttagtacctt ttcgtacgta acacccatca gggatatggt tggtaatgat gaaaggatag 40740 ttatgactat cgtctgagga atagtcagat tgaaaaagac tgcggcactc gtgaccaaac 40800 aaaccgcgag aaggaatatt aacaacaata cggtgtttaa aacgaaatgt cggagtagcg 40860 cagctattag cttcggcgga caacttacca tcttctcgtt ccttgtctat aagaccatta 40920 atcaagccaa gcctgtgagc cgctacaaca cgtctcttaa gcaaacctcc cacaccccct 40980 agctcacccc caagaccttc ttcaagcgtc tcctcgaggt ttgtaggagc tgtaggccac 41040 ttgtttgaca tggctaaagc tttcttaatt tcatcaaagg ttacaggctt cttcctgttc 41100 ggattcaagc ctaaaccact gacgtaggct ttcatgtagt tgacagtgtg ataacggaag 41160 tgtttatcca agaaccgacg aatgtcagca tcaatcatct cagctgtctg tttttcttta 41220 gcgtttcctc gcatccaagc agaataccaa gcagcatatg tgccatactt cttcatcgct 41280 ttacggattt cgtaagtatt ccaaggtttc ttggatgtgt tccactctgg aggaatgaag 41340 ccttcatcca tcaaggcttc tttgatacgt gcatctttcg atgggtcgaa gtctacatac 41400 tcaacgcaag taaacgcccc accgatatct tctcttttaa gaccaactct gtcacaataa 41460 agctgaggcc acttctgtaa agcacctgac ttcttaaaag gtttcgaata agttgaacca 41520 tccacccgca ttttggggag gagaggaatc agttctttat caatattgac aataatctct 41580 ttgagaaggt gaacaaacca ccgagcgttt cttctgtgga agttgacgcc atgcttcgcc 41640 tgctttgaga ttatatcagc gactgtcatc tccgttatga aaggtaactt atcagggtta 41700 tccatcaccc actccttata gttattgata tattaaatca gtgaacgctt ggttatcctc 41760 aacgatatct aactgttcgt aggattccgt tagttcatcc acataaaaga ctgctggaat 41820 atcttctgct cgctgtctac gagcttctct tcgttcgtta ctgttcctct gaacctgtgc 41880 aagtttcata ccccacacct gctcatacaa ctcaggatac ttatcactaa ccactacaaa 41940 gtttcgagct ggttggtttc ggttaatctt cgctgttaat tcaataaact gacgaaactc 42000 ttgaatggag aggttgtcat gaatattgtc ccatttgaga acgcagtgac gttctaggcg 42060 ttgaggtact ggcataactt actccactgt cgggaaaggt ttcagattgt tcatagctgt 42120 acgaacatca tcacagattt cagggtcacg ataagagagg aatttaccat ccttaccacg 42180 aatcattaca ttcgtccgtg ctgttttatt aggcaataaa gaaatccctc caataagatg 42240 gttggtaggg tgttgtcctt gattaacctt gtagtcatga gtctgagcgt caccattaaa 42300 gagatgatca cgaatttgga ttaagctaat cttcatatta tgcaccaaag ttagggaaag 42360 gtttcagatt ttcaatagac tcagcaatac gcttaggaac agccttatca cggaaggaga 42420 tgaacttgcc atcaggacca cgaagggcta cattagtacg agctgttgtt ttgttaaact 42480 gaatcatctc aacagagctt gtaacatttt ctacaggaaa agtatagctc tcagttgtgt 42540 tcagagaagg gaggaaatca gttacagtga tgtaagttac tttagacata tttattacct 42600 taggttacat aaaagaaaag gccacccgaa atgggtagcc ttatattaac ttgagcttaa 42660 agctcctttt ctagacttga ctccgcctgc tgattttcca gcacgatctc cacttttgcc 42720 gccgttcgag cgatttccag agattgattc aacacgtaag ttacttgacg cattagaacc 42780 tcccgacttg attgatcgtt tatgtccaac gtctttacct cggacagcag cttttccatc 42840 gcgcttttca acgatgcgac gtgcacggtg gcgctgagca tctccgctct tgctaccaac 42900 acctgtctcc ccacgtttga ttgcagttct tcgttcttgt gcataatcac gtttcgcctt 42960 atcggtcatc ataactcctt aatatatcag tgtacactaa ggagtataca ttatgtttat 43020 gatttttcag attccattgc tttaacaata gctttacaga aacctgaacg aacaatatca 43080 tccgattcgc catctactag agcggcaaat tcaaataact ggtgacggta gatgaaatca 43140 gtagcccaat caagaccact ctcaccacgc atgtcgtgct ggttagtgtc ccccatgcag 43200 aacatctgag aggtttcacc aagacgtgtc atgattgaat atgcttcatc aggagtagtg 43260 ttctggaact catcaacaat cacgatagca tcgttgaacg agcgaccacg tagatattcc 43320 agaggtacga actcaatatt accattacct acttgacatt cgtagaaacc tttaccgtag 43380 cgctgagtaa taacatcaac gattggcatt aggtatggtt caaacttctc acgaagccca 43440 ccgggaagta atccaagact attacccata ccaacagcag gtcggcttag gataatcttc 43500 tcaatcttcc cactcttcag agcatcaatc actgtagaag tgattacgaa ggtcttacca 43560 caaccagcag gagcatctgt gaatacaacc tgtttggttt taattgcatc aagcagctca 43620 ctctggaatt ggttctgagg tactaccggc ttagcaagaa ctacttcttc aaattcccgc 43680 tttcctttct tggtgacgag agcttcacga cgtaattctc tccgtgacgc tttccgagat 43740 tctctacgac tcatttccta cctccgtgtt cttcatataa gaagctacag gatttacact 43800 ctgagcaatg gatacctata accttaattt cgacaaactt cttattcttc cctttagtcg 43860 gggagccttt ggcaagctcc cgtattactt tcgagttcaa atggagaaga gttgattcac 43920 atttaaaaca tttcagattg atttgtcctt atgtaaacgg tatacttcag tagccataca 43980 agcaatacgc tcgtcatgtg ggagatgcat accagtagag ccgttccatc cttcgaactc 44040 tgaatcaaag aatccaacag gctcgggata catctcttgc aaagtggtta agtcatcagc 44100 cacttgttgc catttgtcat cagaccagat tgggtcatcc aaccaataat acagatagga 44160 atggataagc atctgcgttc tgtgtcgttt aatcacttca gcaggactaa tctcgtttcc 44220 gttaacagtc ctaaatgtat tatcattaat cattccaaaa ccctttcttc taaatcgtaa 44280 aagatttgat ttgtcccatt attctcaaca agcactgttc cattatctaa cagggtagca 44340 acttgggaat aaggaatgtt ctttaacatc agagcattaa tggtcggctt atttttgaag 44400 taagcgacag gctcaacata gaaggaaatc ccacgacttc ctttaattgg agtgctagaa 44460 gatagaaccc aaattgtttc tggcttgtct atttcctttt caacaccaac ctcaacaagt 44520 gagtcagcag cgaaacctac aggacgacta gaagagccaa gtgcccgaat aaatcctggc 44580 aattttgaca tgttagacca actaccagtg ttttcattaa acacaggtct tacgtctcca 44640 tatgcccaaa ccagattatc atagtcacgg gctatgtatt taacccaatc aggaacatca 44700 tactcccttc cttcaaaaat aactttcatt tagataacct tgtgcttata gatgttaggg 44760 ttgaagtagt gcaaccagtt tatatattgt ttctgttcga ggtagtgcct gcaatcatga 44820 caaaaagcat gacccaaacc tgtttcctca ttcagtttga aattgataag aagcttctgc 44880 tttcgacaga agctacatgc tttagtgggc ttcacgtaat gcatcccaag agattggata 44940 tattttacga agctcttcat caattgttga tgcgaacact cgtacttcgt actgagcatg 45000 gtctgacagt cgctgacgac acaactctga ccaaccgtat agagaaccag tccataccca 45060 ctcagtgatc ataccctgag gtaatacgaa tcgagcttgt tctggagcaa tgaacttaat 45120 caattcatcg tacgactcaa gagctgtttc atatgctcgg ttaatcagaa ctagtgcaga 45180 gttttgtgct cctgtggtga ggagattccc tgcacctgca tggaggtccg aaggtctttc 45240 aaaaatctct ttagggatga agagttcaac tgctccatct ttataccgcc gggacatttc 45300 gttccacgag aatccaactt ggtgtttatt gagttgtcta gcaattgcaa tgggagcctt 45360 acaccgaaag gtgatttgag ggtgtcgaaa aggagtcaca tgacgttccc gagctaagaa 45420 tttaatcaac cgctcgtcac gttcgttcag atcaccatct cgtgtcacac caaaggaaac 45480 ccttgctgca ttgaccacag atttgtcgga acccatgtgc tctaccagtg ttgcttttaa 45540 aaaatcagac atacttcaac ttctccttgt tgtaaatttc ttctgcctct tcgcaagaag 45600 caaaagttcc taaatgtata ttcttacctt tgacgccgat aatggctcga tatttatcac 45660 cagaagctgt cttacgaaga tacacacctc taggaagatt tcctatcttg ttatcaacat 45720 cgtactgcct tctgttatga cagttagtag atggattcac atctcttagg ttatcaattg 45780 agtcattaag tttgttctgg tcaatgtggt caatttcaaa ctcagggaat tttccatgaa 45840 caaagaacca agcaagacgg caagctctgt agcttttccc ttcatgctca atgtaacgat 45900 aacctagaga agatacatac ccagcttgat ttcctttaac ccttcgacct tttgtgttct 45960 tccagaagaa gtctccagtc ttagggttgt attccaagtc gtcttgtatc atttcttact 46020 taatagtgaa tctttttata aatcaggttg cttccacttt ctgtttcaat ccctttctgg 46080 attgtaactt tagaagcacc ggtattagca atttttaaag ctcgcttaaa ccggcgagcc 46140 tctttccgtg tggagtatgt tgagaagcta gggattgtgc tatcaaaatt aaaccactta 46200 actacccaaa aagtaatcca aggtttcttt tccatattaa atacctttca gagccgtgtc 46260 taaaaatgca cggagattag attcagtttg catacctgat aaactataat gagcaccact 46320 attcttatca gggacgacaa aggctgggat actgcgaaca ccgtattcaa tggctcgctc 46380 tcgaccatta ccttcagaga tatttacttc ttcaacttct acactgtagt cagccacaac 46440 cttcttaaac acaggagcca tagctgcaca tggagcacat gaattactat aaaacttaat 46500 tactttactc attctattcc ttgttaataa acaggttgat atgacgctgg gtagcaggaa 46560 caacaataaa agatttatac tgaccttttg ctaagtcatg agcaatcttc tttgtctgac 46620 caagacccat cacacagcca ctaacgataa tggcatgtgc ttgtaattct tcaggtaggt 46680 ttagtttcac tctttagatt ccttatagca tacaaagaat aataacaaga ggtagtgtta 46740 tagcaaaatg taccagaaca acactaccta gcttgatagc tttaacatcc tcttctgctg 46800 ggttttcttt aattgctaaa tttcgagctt tatatataac agtaccaact attaacgacc 46860 acacggccca cacaatcagt gaaatcatat caaaccctta tgcagtcaac aggtggagaa 46920 ggaaaggaat gacaacagag aaaattacca gagcaatgct gccatgttta atgggtttag 46980 catcattcgg attttcttta agcatctgtt tctgagccag ataaacgata ccaccaacaa 47040 gaattgccca cacagaccat acgattgctg aaaacatatt cacatcctta taaagaagtt 47100 cgccccacct cttaaacaaa gagacagggc ggaggtttga ttagtaagac acgtcaccac 47160 tagagtggga catgttgctc ttgacgaagt aacagaactt gccaagacct tcattaattt 47220 cttgaagcat actctggtct actggatagg tgcacaagtc tgaatcaaac tcgtccatct 47280 tttcaatgat acacatcaga gtcatgcaca tgtcggaaag cataccgtcc atgtcgagat 47340 cagcactacc ttctctgaac caagcacaac taagatagct actagctgtt gcatccactt 47400 tgatacagtg ataacgcatt cgctcagcta ctcggtctcc tgtttcacga gcatagtctt 47460 ctaattcatc gaacatacca tgaacacctt ggaaaccagc tccagagacg ttccagtggt 47520 aattctttgc tttgatgtgt agaagctcaa ggttagcctg cacaccgcat aggatgtgct 47580 ctagactgtt accgctgatc agtttattca ttccctcaag acgagagata gaggagtcag 47640 ttgtagagaa tgggttacta gaacccactc gtgttagttc aggtaagtcg aagacattat 47700 tatgcattag gagtctctcc ttctaagttg gttgaattgg ctacgttacg agcaatgttg 47760 tccatgacct gctgggtaat aacaatgcaa ttctgtgggt ctacagactt agaacgcccc 47820 atttcagggt cataaacctt aacacgtttg gctgttgagc cagtaacttg caacactttg 47880 agaagataac cttgatttcc aatagaccat gcaacaacat cacgattctt gatataacga 47940 ccaagcatat cacgatgata gaactgctgc ataactttag ctttgctaat ctcttcagat 48000 ggcggggcca ctttactcaa atctaacata actctctcct gtttcataca atattcttat 48060 actgtatata catttgagag ttatcatatc tactaccaac tagtaataaa ccttgctgga 48120 tatgaatcca gcactgttct ggatattttc aatccaacga tgaatctcca ccttttcagc 48180 gattcctaaa gcttttgtag cttttatagt tttcttagca agacgagcct cttgtcttgt 48240 gttgaataat tgaatacaat caccaccaag agctttgtta gcccatttag tacgaactgc 48300 ccataattta tccattagta atgtaccttt ccgctgatat agaaacgacc tgatttaaag 48360 tcttgagctt tgtggatagt gatcttatta attgtgcttt cacaacttaa caagaacttc 48420 ttatgagcgc gagcttcttg acgggtattc ctaataccca ttgactctgt accactgctc 48480 cagtgaatac gaactaccca acgtcttttc attgttccaa actccattat ctgtcaaccg 48540 atttagggac aaactgcatt acacagacct gattacgagg gagatttatt tcacaagcgg 48600 ctcttctcgt ctcaatctta acgctagaat ctggaccaac gttagatata aatactccca 48660 tagcaaatgc cccaactaca aaaccaatag ctaagtaagt agctttttct tctttcatct 48720 tggttataca cctttagatg caactacacc ttcagagtta ttataacgac ccttcactgc 48780 ataggagtgt tcccctgcat gagagtgctc aaacatcacc atctgaccaa cacgcatacc 48840 agcacgaact cgtaagctgt ggttgtgagt aacgttatgg aattcaaagg ttagcttact 48900 gctattaaac cctgcatcag cccaaccagc atgaaggtgg ttcaaaccac aacgagccat 48960 tgaggagcgg agtacaaaca ggcttgagat attatctgga aggttaaact cttccatcga 49020 gtgagctaag aagaactgtc ccggagtgat aacgatacca tcttcaggga tttttacttc 49080 tttccacttg aggttttctt tagcatcaat gtcaacaatg ccgtcagcct gttcttcaac 49140 aaggatagta tcaccaatac ggatatccac gctagcagcg ttaacatatt tatgctccgc 49200 atctaaaaag ccattatcaa taacgctgtg taattcttga gaacctaaaa gactcatgag 49260 tttctatttc cttgtacaga tgatgccaaa ttgttaatca tgttgcgagc tgtttcacga 49320 gatagtgtaa cacctgctaa gttgttatga agctcaccag atagttcacg agcagcagct 49380 cgaagttctg caccaagtgc agtgtcagag tgagccagac cgttacgacg accgacaggt 49440 cgaggagtat tgttagcatc attagctaaa gatggaaaat caatacgagc agcttcacga 49500 gccgctatcg ctgctactcg atcttccgta ggggttggca aatcaccaac ttctacacgt 49560 tctatatcac cagcacatag accctgaaca actgagctta ctgtttccca tgaacgatga 49620 gaacgctgta aatcacccat tgacggagaa ggaataggac gagcagttcc cccttgaaca 49680 tgccatgtac ggttgtgggt gtccgtccca gaagaacgtg cactaccaaa actaccatta 49740 aaagtattac gagggtccat tgtagaaggg gctgcgaatc ttgggacttc ctgttgccaa 49800 gagtcacgtc tgttggttcc cggagtccaa ggttgtgtga ttgcttcagc accgatagtg 49860 gttaatccac tagaccattc tgtctgtagg ttaacttcct cgtccagata actacgaccc 49920 ctttcctgag gaatgtttaa tatagtttcc atcatcttca gggtataatg acgacgctga 49980 ttctgatata aatttacacc agccataggt ccaactaaat cactaatcag gtgaacttct 50040 ggctcaccga aagctcccat tgcaaggtgc atatattcgg tagaactgct ataaactgca 50100 acagttgctt caggccactt cttgtgaacg tacttcattt cacgactaga taccacagga 50160 atgtcacgag tgtatgacat tgcaaacaaa ccttcgtttg acacatacag attaccaaga 50220 gtcataaacc cttgacagat catagggaag cgacggctta aagtatccat atccaaaggt 50280 agattaatca tcatctggat tttaacttga tcaatctcaa tttccagaat ctcttggaaa 50340 gtgaaaagcg cttcagcata ggttgtcccg ttaacaggct gaattagtcg agatgtaact 50400 tgaagcccct tactctctgt ccaagacaag aagttgtcaa ggaacagtgc agcattactt 50460 acgttagcag ggctgttttc ctgacgagtg ttccaacgga atcccccttg aactaggaag 50520 atatcaacat cattcggagt tcgattttgg aagaacgtat cacggatagc accacctgca 50580 atgacaggat agataccatc agggactacg ttatctctga tcaaattacg aattaggtca 50640 agccctacac tgagggcttc tccttcttga aggttcaatt cagacaatgg aatcttaatc 50700 atttctacat tttccagttg agagatgggg gattcttata atcaaatcct gccatttggc 50760 aaagacacat ataaacctta actgttaagt taacgtcttg ctcacaatat gtgaacatgt 50820 cttcagtgaa tgcatcccat gcttcttcct gctcaccata atcacccttc agctcaccca 50880 gaaattcgcc ccattgcttg aggccgtgac cacgagggcc atgtcttggg tcgcctttgg 50940 gatattctgg attgtctcca atacggtcag gcttgagata acgactcagg caaagtgtat 51000 cgaaaacatt gaagtggaaa ggtattacat gagagtagaa tttaaagaga gctggaaggt 51060 cgaagtcgat aatgttatga cctacaacta catcagcttc tttcagtttg tgaagagctt 51120 cttcaatttg gtgagggcga tacccccacc gttcacctgt aagagcatca aatatccaga 51180 tacaccagac attttggatg ccctcataga gattatcacc ctcgatatcg aaggcgaata 51240 ttttctttac caaggtcgtg cctccccgtc ttgtgtgagc agagaagctg catcctcacc 51300 attggtcaag acaaacggat tagcttcatc aacttcatga tcttctcgtt ctacaagtcg 51360 acctgtttca ttgatgtact tggtgtagca aacacctgtc tgaccatagt tacgctcttt 51420 gaggagtcgt atgattgaca agttcttgcc taacccatca gcttgcttat ctcgttcaaa 51480 accaatgatg cactgacacc aacgcattag tgaacgtgaa ccagtgaact gaacctcttt 51540 cacctgtcca ccttcttcgt gaggagcacc acctgccgga gcattcaagt gagataatac 51600 aaaagcagtg aagtctaatt ccttacacat accagcaagc tctgaggcaa tcttactgat 51660 ttctgtattc acttcagatg gggttaggtg agagactagc gcagtgatgt tatcaagaat 51720 gataaactta cactgattct cgactaccca gaagcgaata cactgcttaa tatcatccca 51780 ctcattctga ccgaagttat catacaagaa gaatttatca gcataacgaa gtgcttcatc 51840 acgtagctga gattcctcat attcaacatc aggtcggtgg aaaggtacag atgctgactt 51900 acctgcaatg tttttaacag acatgcctac cttctcttcc aagaagaagc caccaccatt 51960 aaacccatgt tcaaggcaaa gccatgctac cagttcgtgt gcgatcaggg ttttacctga 52020 accaacacca ccaccgattg caaccatctc tccccaacga ataccgaagg tcatttcatt 52080 cagagttttc cacggataag gaatacccca ctccgctttc ttcagagcat cttctaaaca 52140 atcaaagaca gtaacagcac cagcaggtga ttcccgcttc gctgaatatt tcagattgtt 52200 gaataactct tgacctcgac cagcgagtaa catgtcattt gcatctttga tttccttctc 52260 ttgaccattg taagtgaagg ttcctttcgg aatacgagca aatttaacat gggggtataa 52320 tgcacgacct ttagttaatg caatctcacc tgcatcatca ttgtccatgc agagcacaat 52380 ctcctcaaaa ccattcaaga agtcacggtt acgtgagaga acggaagaga ttgaacctgc 52440 accaaacggt agagatacac acgcaggttt gatgtttgta gcgttcgtaa acgcagtgag 52500 cacatggaag ccagacatac aagagagtgg gtcttcaaag atgaagagct tcttgttgta 52560 cacgtcacca cgttgtgctt gagccatacc gaataggtca gcttctttaa cactgccgat 52620 gtaatagaat ttcttgctgt ccagaagctt aacttcataa ccaacaatgt ttccatcacg 52680 ttcacgaggg aagaaataac tatcaggagt ttgcccatcc gtctgactta aaccgatacg 52740 acattcatat cgttcagaga cagctttagg aatcaaacga cgagtaagct cgccctgagg 52800 tagctctaag caatcgtcta agatctctcg gagttcttca ggggatagtt ctttcttctc 52860 attcggtgta ggagttgttc ccttttcgaa aacttcataa ctgccacaac gattacaact 52920 gccccactct tcaccggact cttcgtttcg gaagagaatc atgtgattgt gggtcttatc 52980 acgaccatga gagatacatt ccttacaacc tgtatctcct aggatgatct ttgacatgct 53040 ctatcctcct tggaattcct tgagcagact tcttcaataa aacgaacgta tttcttccca 53100 cacatcatct tgaagacttt taaaaccctg taaagctgat tgggttctca tgataaaatc 53160 ttcagaatgt ttattgcggt caagcatact gacaaactta tcataacggt taatcttcca 53220 ccgtaacttt aagtaatcag actcaagctt tctcagcttc tgtcgctcaa ttactgtcat 53280 cctgttcacc tgccagatac gctgtccaat caccagattc aaccagaaca tcttgttctt 53340 cgtcgatatg ataaatccac gcttccacag tttcaccact atccagcact actggaatta 53400 gtgtacgatt atagaaggta tatgggttgt ctttaccacg ataaccttcc agaccgtcat 53460 atggaccttc cagaccagct tgagttgttt caaacacttc cacacgaaca ggcttaccat 53520 tactgtcatg ttccagagat acacttggga agtatgctgt gcaatactga tacaggttac 53580 agttgtttac tgtccaccct tcaccaatag acacaccacc tgcacgagcg ttgatagcgt 53640 ggttgtgttg acctttacga agagaaccat acgttgcaac taatacttta tcagacatta 53700 cattaccttt tgtttagaat ttcttttgta caagaagtcc cgttacacac gagaattgtt 53760 tgagttacag aatcgacaac cgttacatgg ctgccacatt gagttataat ttgacctctc 53820 cctgaatctt tagagatgca ttcatcagcg gcgattgctg atacgctgaa taaggagagg 53880 aggagtaaaa tctttttcat gactgtttac caatcgtata ggaaaccaga atatccggag 53940 aaaatgattg atcgaacatc tttttcagag attagttcaa aactcttctt aacagcttcc 54000 acaagatcac tgttcgacaa ttctttttcc ttgaaccaat actcttcaaa aatatcagag 54060 aaggtgtagg ttcgatatct ctccaagtca taataatggt ctactgcata gttaataact 54120 ctgctgatag agctagaagc cacagggaaa agggagctgt gctccataaa caggcaccag 54180 cgattaaaga gtggtatgtc ttcatctttc agaagactct ctagctcttc ctttgcctct 54240 ttcttatgct tctcaaagag gtttttggaa tactctacgt ctaagatacg acttttaaac 54300 atatccagtt tattatcaac catttacaac ctccagttaa attcgaacta gcctcctctt 54360 tcgaagaggc taaagcaaat tattctactt cgaagtgttt gatcacacca gtcaacacat 54420 cagattggaa tgtacctacc aactccactc ctcggaagaa gttaacactt gttccaagcg 54480 attcaaaacg ctctgcttga atatctagag attgaatatt accttctgag tttactgtac 54540 gaaacatgat tgtgtattta ttcacagagt tgttactccc ttatcttctt gtgcaattgc 54600 gatgatacgc tcaacacatt ccattgaata tttatcacct cgtttaagga aggtgacaat 54660 tgcagagaac tgattaccaa tcattctctt gtttgtgatt ccaaactctt cacagagttg 54720 gttaatagct ttacgagaac caacaatcag aggttctttt tgttcggtca ttccacgact 54780 gatcaaatct tcgatagaca acattacaaa ttccttttgt tttttttaat tacagagtta 54840 ctgctacttc actaccttca acagttacag actctaactt acgagggtta ttaatctgtg 54900 ctggatggac tggaacgttc caaccactgc ggtagaagtt acgaccatcg cagaacagaa 54960 tgacattggc agacattgca ttaggttgca ggttgttgta aacatgacga atatttgcaa 55020 tccagtaacc acgagacaaa gtataaccag taggtgcatt acgaatagtt tgagtattca 55080 tctgaccttc cagttgcatt tcttcaggaa ccacatcaac tgaatcaacc ggttgtgcta 55140 cttggccttc cagtgcaaag ttttcatcgt cagcactttc actgtcagca accaactcac 55200 cgtctccgtc ttcgattgga gcttcaacag gagtctggta aactacttta tactgaggag 55260 tatagagagg gttttgagct ttaacaaact caaggatgtt gtgacggtag atatccagag 55320 ttgtgccagt taggcccggt gctgtattaa cttccagtac ccatgcttct tgacctttgg 55380 tgatgatatc aactgcaccg aaatctagac ccattgattc aacagctttc actgcgttga 55440 tcagagaggc atcgttcgga acgtctgtga aggaacttga gtaaacccaa ccagtgtgat 55500 ggttacgaac atcttcacga tagttagggt cttcacgata accgttacga cgaatctttt 55560 tctgaacata ggtgatagca cctttgaaca catggacacg ccattcacga cgtgggcctt 55620 gaatgccttt agtgtacagt tcagctcgac caagaccatc acccggttct acaacaacaa 55680 tgcccgaacc agagtgacca gtcagctcat ggcgctcata cacaatatta ccagtgtttg 55740 accactcttg agcagtctct tgaacatctg tgtattccac agttttaaca cctgcttggc 55800 ccatcacgct gaaagcacga cgtttgttcg ctgccatatg taaggctgcg gtacggttaa 55860 ggacggttgc agaaccaaag aatgattcag gatagcgacg gttcccgtag ttaacaacca 55920 catcaccttc acgagaacgc cagcggctat tctctgaaag aagacccatc acgttagcat 55980 tgatgcgttc atcacggata gtatcactga tagcagatac cagagcgtta acagaatcag 56040 acgggccata cggaagaaca cggatacgag tagtcatttt agataccttt tagtttaaga 56100 atttcgttac gacaggattg aattttaact tcgattgccc aatactcggc gaacatttcg 56160 tcttcttggt catcgtttgg tgaatcaatt aggttgaaag caaacggata cagctttcgc 56220 tgctccgtca ccatttgttg aagtcggagc tgttgatact ccagcattgt caggtgaggc 56280 ttagctccct tgcttgacat gaggctcaac tttaatcccc ttcattttca cgtagtcagc 56340 caacacgttg ttaataacgg ttggaaaagg gatttcaact ggagagtcaa ccagcttccc 56400 aataccttct tgcatccact tctgaatggc tcgaaggtta tcacatcctg tgcttgtcat 56460 tgggatacgt cgagtagtta caacgttatc caacatcaat ttacgaactt ccataacttc 56520 acctcttaat gtttaccttt acagtaatct gaattacaga tcaccctgtt ttctttgttt 56580 agtgagcaaa tttctgcatc acgccacgga atttgtttat aacagactgc acaaaaccca 56640 attgaagtcc agagggcttt tgtgaactca gctgttcgtt gtccgtcgta actgacaact 56700 gtttttcgga gagttgtttg cttttctgga aatgtctccg ctttctcagc gggcacctcc 56760 tttggggcat tctttggacc ataaacagag atggcatagg tatcgccacc acgtttttcc 56820 aaataagagt ttggaacatc agacaattca atgtcaccag aacgtttagc tgagctgacc 56880 attacaatca tccggctaag catacgctga tcaacacgaa ctgctacttc agggcggcgt 56940 gggttagttg tgtcaactgc tttagtccaa atgtaacctt cagacaatgt agtgttagtc 57000 actttgtctt tgatttcttt ggcacagaca taacaaatgt cttcaccacg cagtttactc 57060 atttcattat caaactcgaa atcagtttta acattaccac acacagcaca gacacccaaa 57120 ggaatgtctt catcttcttc agatggtttg gagagtgctt cgaaacaggc cggatgccaa 57180 tatgcttggt aagcgaacac agctttctta gcatctttcc aatcaatgtg cttatcacaa 57240 ccaccacaat caccgtgaga atgtgattcc cagaacttac gagtgacttc tacaccattc 57300 ttagtgatga aggagtcttc taagtcaaaa gggatatcat catcaaaatc agcggtggtg 57360 ctttgaggct ccttatcggt agcgtcaaca aacagccctg aagttagtac gaggcgaatc 57420 atactgtcca cttcggagat acaagcaact gtcccgcgat acactacctt tgggttttcg 57480 agagccttca agtactcact cttggctaca ttatgaacat caccctcaac atattcttga 57540 gcagcatcat cataaatata accaaccatc ttaccacggt cagtgttgtt gtaagcatgg 57600 aactcgtgag ggatgatatc cacacgacca tcacgacgat atggaagacc acgatcggca 57660 gccagtttat tctgatcttc aatcgcttta cgacggcgaa tgtctccgct tgtcacgccg 57720 ggcttgtcgt ctttgtttcc gtaaatgtcg cttgtgctgc tgcttgattt gtttgtctgt 57780 tggctctgat atagtgagga gttcgagtaa tagccgcccc agcgactcgt taccgtaaaa 57840 accggaagct tgtgctccac ctcttgagtc agagtcatac ggttgccaga caaatcaaag 57900 atgtattcag ttcccacttt acactcaaag tgatcattga tacggttctt gtgtgacttg 57960 gaacgattaa gaatccacat caacatatct tcttcagaag caccaaacca atccagacca 58020 caacgggcca gatggaaagg acgttcatcg ttacgaatga tatgcagagt attgtctttg 58080 gcgtcatgcc agattaaagt gaacgcacca tccaacttct ggatagtctc tgctgcacca 58140 atggtgttga ttgaatgaca gatgttttcg ctatcaactt cgaagttctt atggtcaggt 58200 aacagctctt ggtccatcaa tgtaccattg tgaaccagag taatgtcacc atgagagaac 58260 gggtgagcat ttcgagcatt gacagcgcca cgagtggcat gtcggctatg accaacaatc 58320 cacgctggag gaactacata gttcgtaagc cccttagtaa tattcgtgta ctctgtctga 58380 agcatgaaag cataagaagg aagtgcttct ttataagaaa acactcttgc atcactttta 58440 cgctgaccga acacaccagt tgagtgctga ccacggaaga ctccggcata cagaagctgg 58500 ttgaatacgt ctaagtctgt ggaggtcatg ttgcccccag ctaatacgat accacaaatt 58560 ttctaatcca cctgaagggt aaaccctttc atctaattaa cctttaaatg cgccctttat 58620 cagagcgccc attgtattac agcagtcgtt ctaattgcat ttccagatgg tctggaatat 58680 aaccagtgac gttatcatca ctgctacccg attcacgcat caatgcaaac atgtgacgaa 58740 tttcacgaac cgttgcgatt cgagtgcttt caatctgagg ggtgtaatag gtcatgatct 58800 cttcatagac agcgttggca ttaccacgaa catatcccaa gaaaccatag aagtcaccca 58860 tatcacgcaa gttgataagg acttcaccat cagggccagt ctgttgtaca cgaacacgac 58920 caaccagagt aacagaatgt tcacgacaga acagaatatc acgagccaaa cgataaccac 58980 cagtcaggtc atcatcatta ggtgtgtagt ccagaggcag atactgaatc ataggagcga 59040 agcgtagctc attcagtttc tcaacatatg cttcattgtc caaatctgca tactcaacag 59100 ccagtttctt aagaaccaag aagcggttga ccagattaag gagttggctt ttcttccact 59160 ttggctctga gatacgaaat tcaacagaac caaactgagc cagaggtaat aggttaaggg 59220 aggtgtactt gtcccagcta ttaacaagag tattgaagaa ggcttcacca tctcggttga 59280 acgctgagga agcattcatt acctgtgctt gaacaacagc aaaggcagga cagaagttgc 59340 tacgataacg atgatatcca gaacatttga acatcatctt ttcatagaac gtccatccaa 59400 gaattacttt cttcaggata ttactatcag cgtcacgcat atccatatga acgtgagtag 59460 agcagcgcca agtcccttca gcttcagagt cttgtactgc ttcactcaga ttgttaatag 59520 cactaagcaa ttctacaccg ttgtaaggcc gtgcacaaac catctcacag ccatcacgaa 59580 gtgaaccatc ttcagtacag acccaaagat ctgcatcaat ttcagaggtt ccttcaactt 59640 ccagctcgat gccaatacca cactgaaaag cgatgcttgg gtgcggctca gccagacgag 59700 cattacggta tccaaaaact tgtgctacgg ttgtcataac gtttccacca actctacatt 59760 atatgatgac agatctgaag aaatatgctt gaacttctcc agaaggttta aagattgacg 59820 accattgtcg ccgttaccag cagaaagatc aacttgacct acaaaagccc ctttgtagta 59880 aactttacca tcaacagggt taatatagat gaagcgagta accagacctt caaagtcagg 59940 gttgaacagg ttatacacca gctctcctgg aatcgaacgt cccattgaag gaggtgcacc 60000 acgcagcttg ttccccatca gacccttagc tcgctgacga acagggttga tatgagtcca 60060 gcttaccatg ttgttaccat gttgcagata gcccacatct ggagattcca gaatcaggct 60120 ctgatcaaac acaggaatac cctgaacaac ctgtcgctca aactgccctt cggagttctt 60180 agaccagcgt tcaacttcaa aggttaaggc catttcggct tcgtgagaga agtcgtcttc 60240 cccaaagcgg tttggttggg ttacgttaag cacatacaga gggagattaa tccctcgttc 60300 tgcgcgttgg gcaatccatg ttccgcaata ataatgctga aaatctctta acaggctcac 60360 gataacacct tagataatct gaagttgaga ggagaactta tccgcttctt taacatcatt 60420 gctgttaatg atgttgtgaa gcttttcgat aggcaaacgg gagtgcagag acatgaggat 60480 ttccatgtca cagccctgaa taaccttatc aacttgatcg taaacaaact tacgagtgga 60540 ctgttcacca atccagaagt tcgacagaga acgatattca ataccgtaat ctttataacg 60600 aatcgccgat gccttaccgt aaagttcttt acgacgagtg tccttgtcca tcacaaccgc 60660 tggaagtgac aggaagtaat cgcacagcac acccagcatc atctgagtct gacgactaat 60720 ttccagtgta ccagtaacac cgaggtgaac gtgacctcca gcggtacgca gacctttgtc 60780 tgtcgctacc ggagatgggt ttttctgacc agtgaaggcg ttgaaatcag gagtacagcc 60840 aaatacgaag gctgacgggt ggaatgattc cagctcttca gcagtataca catgagagct 60900 aatcccaagc gcagcttcca taccacgttc atggagaact ttgttggtca gatcaatacc 60960 acgctggatg ttatcatcaa acgcttcaaa ccctttctgt gggttaatat caaattcagc 61020 cagaacgttg tcttcttgaa gacgaacatc aggggccaga tcgattttgt tatctttaga 61080 acagcccagc ttaccagcca cagaggtgat aacaccagaa gcagtacgaa caaacatttc 61140 agggtcagaa ccaataagga aagcacgctt agtcattgta atactccaca agttcatcaa 61200 aaagggaaac aacacgggca ccagcttctt ctggtgtcat tgaggaaggt actgcttgca 61260 tcaagcgttc aataaaactg cgaacagtaa ttctgtccat acgctgatct aatacttcac 61320 gtttatggcc tgcacacatt gaccagagac gtaaccactc actcatactt tcataatagt 61380 cagggtcttg gtcacgacga cgggccatac tacgaagaac tccgtcatta tggtttcgta 61440 cataaccatc tctgttagcg tcaataccaa atatattctg aacccatacg ttttcagaaa 61500 attcatcagt tgaacagacc agtggcaaaa atgcgattgc actcatttca ccggtaaaat 61560 aggtcgctgc tgattcatca tcagtatcca catcatgtga ataatcccaa cgaccaaaga 61620 agttacgact taaaccagct aagcgtaaag ctaagccgat tcgttgtcgc ttgagtaact 61680 cttccttgtc ttgaacgcct tcctgaagga actccattcc cataaaatcc ccatccagat 61740 aactccggag ggtcattaat ccagtaaaca ccttgtctgc tggatggtct cgaaggttca 61800 cggcaactga gttcaccgaa gtgacagaga acatgttttt ataacaagac attgagttaa 61860 aaatgtctgt tgcttcaact aaagctcttt cgatatcttt agctggtgtg ctataaaagt 61920 tgtgaggcca gaagttaata actccaattt ctgaagttct atcacgaagg cttgagagat 61980 tgatactgtc gccattaatg tctgtaacag aatacatatg ctgacagttg aaagtaccaa 62040 aacaagctcg ccctctgaac actgactcac caaagtcctc tgatggtgtt accggagcag 62100 tcgatgcgga gattgacata ttcgcctcta tttattgaag aataattttt ccaaaatgtt 62160 ttcccactct tctgagggac gtactgtata attactacct gtcagtccat tggacatact 62220 actgataatg ttatacatgt tgtagcttcg agctgaagct gtttcattgt tccagttata 62280 accgaacaca attttagcaa aagatgtttc agattcagcc acctctcgga taaattcaac 62340 caagagtgac ttttctgtga tgattgggcg ggtatcttcc gacagatggc aatattgagg 62400 caaaaggatt ggcaagaaca tcgagttgtt cactcgacaa tcacgaacac cttcactgcg 62460 atagatcaga ctggagaagt cttcatcacg caggtggcct tcaccttcag tgtattcaca 62520 ctgtcgccat ttggtctggc gaggctcaat catcgtacta ccaacaccca ctgtatacag 62580 agatgcttgg aagatacagg aatcgtaatc attccctgtt aaagcaacac gttcgttacc 62640 gaatacattc actgaagagt tgatcagtcg gctgaatgca agagaggcta aaggagacca 62700 gcccttctta tacatcttgt tcaacatttc atacacagca gatttttcat catctgtata 62760 gaatgtgcga ttcaacatca gatagaacag agcacgatca gcgggacagc catcagtgca 62820 aacacccatg aaccccactt catgccacac cagacatgag tcagagtctt tgtccataaa 62880 tgctgcaaag gtttccataa caacacgatc agtgatgtca ggacggttgt tttcttctgc 62940 ccatgtttcc acatgagaag gaggacgagg gtcttcattt tctaaccctt cacggaaata 63000 ctgagtagcg atatattcag tatcaccata acgtcttaca ccgtgagctg gaagatgtgt 63060 tccggagttc cagatatact tgatttcaga cataggcaac atattctcaa gaacgttctc 63120 ttcagcagtg ccttgacgaa ccatcatatt tttactccac accttgatgc ggatattcca 63180 gccgtagttg ctgtcatacg tgttgtagta gtcaggatca tcgtcttcat catcttcatc 63240 ataatcacca ccctgatagt aacctgcttt aaggaaacca taacaaggag gccagacggt 63300 tacaacatct tctgattctt tccagctttg aagtgttaca gggaacatct tttacctctt 63360 tactctgcta ccagaaggcc cagagatttg cgaatgtagg caatgctacg gaagtttcgg 63420 tagttttctt ggaagcgcat tttttcgaat tctgcttcct gaagttctac ctctgctgca 63480 ttgtatcctg ccacagccaa actcaccaca tcctgaggta aggtattacg acgacgaata 63540 agatatttat tcgcctttaa cttgctttgt gcacgagcca ttttggcctt tgccgccttg 63600 atgccatgag caatccagag caattgctgc tccaaatcat tctcgtccat gccacccaaa 63660 tcatttgcta ctttatgcag cgtagacagg ttcacatggc ctttgatggg gcgtaggttc 63720 ataacttctc ctgtttatca cattgattgg taagccagtt ataagaccct ctgttgttct 63780 tgcttaattc acaagataca aaggaagtca cacgggcaaa ccccacggtg gctaagcaaa 63840 taaagattgc tgccaataag aacttaactt tcaacttatt atccatacaa cctcttcttt 63900 gaacttaaaa aaggactctc ctaagagagc caagctttaa ggccaattaa atagatatca 63960 cccaatatct gtggataacc tgctcttaaa atattctatc cgacggatgt tcgaggactg 64020 ttcctctccc tagcatcggc cccatacggg taagattaaa ttaagtttag gttagttgag 64080 ccagaatagc tcacatagcc ctctgatact cacaagggct atagatgtta ctctgtggct 64140 ccgtcgaagc gtaggattgc tgttggaaca ggaatcacaa ccatgtcaat ctctaaacgg 64200 aagagatggc ctgtgcttag ctgaactgct gttaccggtt tagcgatacc accaacagat 64260 gctgagggga ttcgaataaa gaaatccccc ggatattccg ttgcacgaaa tacatctccc 64320 ggtgacatat cctttacttg ggtaattgat tcaggagggg ttttattagc aatttgcatt 64380 acagaccttt cttagcacga tctttggcag ttttgccagt gcggatacgg tgggaaacat 64440 taaccagctc gccgccttcg acgatggtca cgcttttgtt tttcgcaata tcaaacacag 64500 acttctctgt accacccaga cgaatctgac gcggttcact ttgggtctga gttgacttgg 64560 attcaaactg gaacggctga cggatggcat caattgcgct aactttagac atgtttttat 64620 taccttaaat taaagagaga ttaaacaacg aattggaacg cttcgtatcc aaacttgtac 64680 gagaaaccgt gcttgaactt cactacacga ccacgatcac ggaaagcttt cagacgactg 64740 ctgaacccag ccatcggctc agggtcaata gggctgcgaa tcttcatagc tacagagtgc 64800 agcttgaagc gcttaccagt gctacaggcc atcatcacgt actccatacg agtagtccag 64860 atgttaagca ccatgcttgt tttctccgct gaccaaccag tcttgcgggc gatttcctta 64920 tggatgttaa gttccaaagg aagtgggaat tgcttgttga atgaagccgg ataaagcttg 64980 accagaagat caatgttttc tttatacgtt aagtaagcca atttcttgct tacgttgggg 65040 aatttcccag ccatttttga agctccaaaa cagcgccgaa aagagaggcg tcatagtagc 65100 taaagggagg agagaagagg gcttatttac ttgccttttt ctgtccgtta atgtaccaga 65160 taactgaacc atcaggctgt cgttgaccga agcctttcaa gttaaccatt tcttcttcag 65220 agtaggcacg ttttctcact gattttctcc tgtcagtaga aggtgtgtgt agagtcgtcg 65280 ttcatagata accaaacgaa gaagacaatc acacagaaag caataatacc gagagttgca 65340 aggattatag tgttagctaa tgtgatatct aacactcttt gcaggtacat aaagaattgt 65400 atgataccac ataaccctag cacgacccca ataacaataa ggagccagta cgtgcagaga 65460 aggactgcat ccaatagtga tacatacttt ggatcatctt tatgtttcac taatcttctc 65520 cactaaatcc agagttgccg attcaaatac aacaccaact ggcatattgg atgccatatt 65580 acgaacatat ccactctgca tcgacacagc tcggtggtat tcacaaccat tagagctttt 65640 gtttactttc atgtaaatgg tttgttcttt atcttctttc aaagtaaaga aagagccgat 65700 agaaacacct gccaccatta gaggaatatc cgcaatacga gttttgttaa tggtcagagc 65760 cattgcttac tccttaatag ttgcttcagc tttgatagga ataacagcaa catctttacg 65820 aggcgctgtc gctcggcctg tgttaagaca ggctacgagt ctgtgctcat ccgcaccttt 65880 aatgcagagt cgagttgaga actcggtaga cagcaagatg tataaatcac catcttcact 65940 gttaggatac ataaagcaat ccccaagtcg caaagcactg aggggtattg aatttttacc 66000 tgctggcatg ttaactttca tttcatcacc tttatttgtg agagtttacg aactcttcaa 66060 gaacattaac tgcatctcga tatgcattgg taactttata ccagtgatca gtaccgaatg 66120 cactcggttt gacgattcta accagacgac ggtgataacg cactgagtag actagattct 66180 cataagtcac cctcagttcg tttttgcgtt ccatctgaat agatttaagg tgtcgctttg 66240 ttttccagac tgttaccggc ataaccctct taccttgttg taaagtatgg accaatttat 66300 gaaagaactc tcggaagaat gctttcataa atagggggag gggacgaatc ctcccatatc 66360 ctattaatta atgttgcatg taagacctaa caatccgatg ctgtagtgga tagcgtaaaa 66420 cgcttaccgg cattaaaccc agcccatgag gggcttatgt gattaaaatg ctcaacattg 66480 agcggttagt tgataggaga tctgaagtcc tatctcaaag gactctcgta agaatccttc 66540 acgatattac ttagtaataa agactgtcac cttatccatt acccagccta tcaacataag 66600 gaaaggtaag aagaggataa agatcagcat aaaaggccag aagaatatta ccgtccaaat 66660 agaatattca tcaggcttac cactccatga ggaatggaag aagttgtaga gtgtattggt 66720 tttgaagcac catttaacta caatggctgc taaccaccaa ccaataacat aaatcaggag 66780 gacatacgct gctgtaatca ttcaccgttc cattgatagt tgcgagttac cacgtttccg 66840 tggataagga agtgctggcg gcgaaggata tctttaacgt tgtagtaggc agcgttaccg 66900 agatggcctt ctttaataag ccaacgaaga ttacggatat caaccaacag catttttact 66960 tgctgttggc atgaagtttg agctgctgtt tcaagtagag ctttcgtatc agaataatga 67020 ccggacatat caccactcct tacctgttgt tgtcacagag ccaccacaca gctcaccacg 67080 ggagatgaag tcataacgtg accaatcacc ttgggtagcc atatcacgct ttgcataagc 67140 cttctcgtca acagcaccgc cattggaaca agaagcacca gcataacgca tatcaatttc 67200 accactagga atcttctccc cgctgtgcca acgggaaaca cagacacctt ctaccgataa 67260 tgcacaccat ccatcaggac gatgctgtac gcagcctgta agagctacac aggcgataaa 67320 gatacaggtt aaggtaatta caccacccca gctcatgatc cctcctgaag cgccttacgg 67380 cggctatctc gccaagccat ccaagtgtag attttgagtt cttctctttc ttcttcactg 67440 aacaaacgag gagtgaagat atcatcaaac catttattaa actcaggggt catataccct 67500 cctacgttga ttggtagccc caagatgccg tatcttatcc aacgacccca tacagaccta 67560 taggctattg tatggcgccg aaggagtacg caagcatctt agggacatat ggtgcaccct 67620 acaggattcg aacctgtaac caacgactta gaaggtcgct gctctatcca ttgagctaag 67680 ggtgcttgaa ttgtttataa ggagaaagac ttcttacaat ccttatcttc aaaagtgtag 67740 tagcctgttg tatcagggaa gtaggcatac tttgtttctt tgccatcagt agccatgaca 67800 cgattaccag aagttgtttc tacaaacaga agcccatctc ttaccatacc attagattct 67860 ttgataccag ttaagctatg ctctttaaga gtgcattgct gaacactctg atacttaact 67920 ccaatctctg cactattaac agagatgata ccaatggttg tcattagtag taaggttgag 67980 atgataaaat gcttttgttt cacattaacc tctggattta tttaactgaa gatacgcaag 68040 gccacatcgt gatagatatg accggggact ttgtgctgtt ctattttcaa acagcgttta 68100 cagacacgag atacacgaga ctctttcgct tcaactggag tgtctttagc gtgagtgata 68160 tgtaagtttt cagtgatcac ccactcgtgt ttacatccag aatcttcggg ctttttctta 68220 cgaaagaaca taacacctct tagatcaaat ctgttttctt gtgcttacca cacactttac 68280 agcgggagta gatacgcaac ccttgctgct ctcctccagc aatacgatgt gtaaccttta 68340 gttcataagt agaaagatct tcccactcat ggaaatgaaa gaaacgtttg aaccatttaa 68400 tcataacgat tccttagatt atgtcacgat gactaacttc accacacact ttacactgga 68460 tatcgtaacg tacgcccctt gaatctccac agatcagcgg acgggtggca ataatcttcc 68520 aatcatgaca atgaaagaca cgctttatcc acgtaatcat attaaacaca cgccggtagt 68580 tcaattaact gaggagcacg acaaggacca tcttcataca caccaaccaa cgttggcatg 68640 taaggcccag cgcactggta ttcgtcgaac agttcgttat agttaggctt ccacttccaa 68700 gcccacacat aacccattcg atcaatggca atgtaacgag tgttcagagg aacctgcatc 68760 atcacatcac caactgctat tactttcgta ataactgctg ctcggaaatt tgtcttcatg 68820 gcgctttaat ctcaatctga acattgacaa caaatacagt atggtttgca ctgtacaccg 68880 aagtgcctat gattggcttg tcatcactga ggatttcaac agcgtgttca aggttgatct 68940 tcagatgaag acgccctgat tccatgaaga aatcaccagc acgtatctca ccgaacttgg 69000 ctggcacacc gatagagggt atcagtatgt cagtgcgacc tttacggtca aataaacgat 69060 taaacatatc aacctctctt gttagtttaa gtaagagtta tcccttaatt aaaacaacaa 69120 aggccacccc gaagggcagc ctcttattaa gcactacgca gtttgttatt caagaagcga 69180 tcttctgcat tatataggaa tgcatagaat aattcttttg ctacatcccg atcgcaattg 69240 agacatttag aaacctgtaa caacaggcca tcgcgactgt taggatggtc aattatcgca 69300 agacgaataa tctcacggat accaacatca tgctcagccg ccatagaacc aatcaccttg 69360 acgcttacgg atgttagtgg tcagacgaag catggcagac tcaatcttca gagcaccatt 69420 tgctactgca tccatacgtt caggacggtt catacgtttg gttgtctttt tcataacgtt 69480 ctctctttgt taatggaggc aggattgcca gttaatataa agggaagccc tacacgttaa 69540 ggcttccgct tgtacaaact gtcgagaact ctctacccct agattgttct ctatcccaaa 69600 ccgatacata cccctacata tcggaatgcc attcgaaaat taaacaggtt ttagtctatc 69660 accggatgtt tgagacaagt ctcgccctag cggtgagcca tatggcattg agattatagt 69720 taattgagga acatcctcac atagccctct gataaacaca agggctataa gtcgatgtta 69780 cttattagtt agtcgaacgt taggcacaag tttaactgct gctgttttca tctctccaat 69840 gaccttaccg gtagagagat taacaaaata agcagtgact tcaccaccac aagctgtacg 69900 catataataa ctgcttccag ttctgtcaaa ctgaaaaacg tctcccggct ttacatcacc 69960 aagagttgtg acgctttgct ttttatcaat atcaatcttc atttaaccac caatattcgt 70020 tacttgtaca ttaggtacag agataacttc atgatcttca ggtatctgga acaaagtccc 70080 ctgaccaaga ccaacgacac ggcgcatacc ctgcacatag gtgtctgtct ttaggtagag 70140 gtgttcagtg ttattttgta atcggaatac gtcacccgga cgtagatcgc tgagcttttt 70200 agtaacaact aaagatggga gattaatttt cattacttac tctcgatagc aattttgtat 70260 tcatacttag agacttcagc gtccgcagaa accttaagta tatcagaggt gttgtttaat 70320 tgcattgccg atgaatattg agatgatacc ttcatccaga aaaaggtgtc accatcgggg 70380 ttttgaaact tgaagatgtc cccattagag aggtcaccga aagttaactt attttgttta 70440 ggcttgggga tgatttcgaa attcattttc atttgattaa caccaaatca gtacgacgtt 70500 cgaaggaagg ggaagactca ccattccact gaacaagata acagagaaca ccatcaatta 70560 ttgttctctg acgaataaca cctgtaatag ttccaccaac aaggcgaacg gtgtcgccaa 70620 tggtaatttt aacctttgag cgcatctcct gtttcctcat tctggcgttt aatttcttca 70680 gtgagttgct tattgagatc ttgaatacgc ttaatctccc aacccaaaga agccaaacga 70740 tcattacgtg cttcgatttg ctcctgcatc tttgccagct taagtcgagt agcatcatta 70800 aagtcttcct ctagcttaag aaggaactcg taacaacgct tccagcttgc tgatttattt 70860 cttaaaataa taaatcctag agtagaaatc gttacagtga gtacgaaaag aaatttataa 70920 acctctaaag tcatatcttt atcctcttaa agtaagatat tataagaaat attatttaga 70980 atttattatt tctaaactta atttctatca ataattttca gaggtctata tacagtcatt 71040 acctctggtt ccgaatcact accacttttt gcagagaaaa tcataagtag gtgatttata 71100 aaggaattta gtttgccaat cttggcacct atgaatcagt cacgggcact attattgaac 71160 actaaatcac cacaatacat aggattggta taccactttg gcaccccttc actcttactt 71220 tgaaagtgtg taccgctgta caaaggcaca taatctttct tcagtgcacg tcttgcaagc 71280 cgctcgctct ctctgattga cttcttagtc agagggttat catcacgaat cagctttaca 71340 taacgcttaa aaggtttctc cttctttaat aagacgggta tccactcgaa ctggtttcgt 71400 tggtttataa cctgctcggc agtcagtttt cgttgtgaca ttctgtttat tacggtatcc 71460 gctactaatg ctttgcaagc ggttggctga ttccctgcct cataataaat agtcaatgtc 71520 aaaagtagct gggctgtcgt tatcagaact tactaccccc ttgttttacg ggcataaaaa 71580 agccccatcc gaagacgggg cgacctgaaa gagtgaacat gacctcactc atttaagaat 71640 cctcgcaaag actcttaaaa aagcggcctc gttaaaggcc cggtcaaatg accatccaat 71700 agggattgaa ccatgccaat gattcacaaa gggagttccc tttaggcatc actagctacg 71760 caaaaagccg cacaaaggcg gcagacacaa agacgctcaa gaagagcgtc atagtagttg 71820 actactggta cagagaagag gcttacttca ctctcgcttc cttgatgggg tcaatgtatt 71880 tttcaactcc ccatttatgc tgactatcag cgtagaaaac atccggtgca cactggcttg 71940 tcttagggaa agcctgcggg tctttcgtgc tacccttaaa cttctgttta ccacgggtgc 72000 gaccattaca gaagatttct aacttcttct cttcttcggt tttagctccc ccgctctgta 72060 acaaagaacg tttatgatca gctaacaaca acgcatcagc acgatggaca cgatggccta 72120 cacgttttgc cacactggaa ggaacatggg attcagcctc agcctttgca gcacgacgtt 72180 ttaaactgcg cttactgacg tgattggttg ccttcgactt ctctttctcc agttcagaga 72240 tagaaaaaca acgcccgccc ttcatgctga ttttagattt agccacaatc ttattccttt 72300 tagtgataag ttatatcggg caagcccatc aaggcgatag gcttgaacaa ataacttaat 72360 caagtaaacc gaatgacttc caactgcgta gcaggattgt accgtcatcc atagttacac 72420 ataattttag tagtttctta ttggcactac tcaccgttgt aattcttccg gttgtgcatt 72480 tacctttatt gctgtaaaga tatacaacac gacggcctaa cagtcgggaa ggagctttct 72540 taaattctga gatgttagtg atcataatgc ttgatacctt tgaatgtgtt caatacgggt 72600 agattgagca aaggacgctt taacagccat tttcctaact tcttctttac gctgattggt 72660 gtgtacagta tcccaatggt tgttatcacg ttggcgctgt aatatcttac tgcatttgcg 72720 atggtttccc gtgctgcgat gtttctcaca aataggacac ttgccaaaat tgctactgaa 72780 tatcattttc tcaatcccaa ctttcttaag atacggccta acgttgtttc ttgcgtgata 72840 atatagctga tcgcttcacg ttccaacgtt tcgatcttca ttggcgacgg ataccattca 72900 ccggtaatca cacatttaca ctcaacgcca tactttttag ataggcggta atgttgaccg 72960 ttgacaaggt ttctataaat cgtggtatgc gtaattaaca tgatcatcac ctttgattaa 73020 attctttgat taatgcactg aatcaatgca ctgataaaag aaaaggcgct acccgttaag 73080 atagcgcctt atgtttattc aaattgtctt acttatgctg cctgacgtgc tgccataatc 73140 tcagccgctt tacgcaatac ttcttcctct tcttctttcg cagcttgttc ggtacgggta 73200 gcaaccgttg aagagatacg accgcgcagg ctttctaact tctcctgcat tttggcaatg 73260 gtggattcca tttcatcggc atacgaatcc agtgattcca gttccttgct tgatgcgttc 73320 atcagataat catcagagat cgctaccgtt gaagccgtga ctttcttgat gagtgagtca 73380 aaagacttct taaccttgcc tacttcttca ctcagggcaa tagtattatc actggtagcg 73440 ccgttacgtt catcagccaa taattttgct tcaatacgga ttgcatttaa gcgttcaacc 73500 aaagcgtaaa tatcttggtt aaaatcttcc ttgtttttcg ccgggatttg caggtgtgcc 73560 gccacgttgt cattgcaacc cttgtaaaag ttagggttat ctacttcaat ttcacgacct 73620 aacgcaaagt tttcgaaagc gttcgaccag cgagcgcgat tctgttccat tttggcctta 73680 atttgcgctt ctttcgcatc ttctttcgct ttcgcttcac cttccagatt aatccacttt 73740 ttacgaccgt ttgccgcatc ggtgttaaac ttgacaggag tcaggaaagt gattgcagtg 73800 gcgatcacat ccatatcacg aaaacgctcg gttaacagaa aatcgaccgc cgcctgcaat 73860 acgtctaact ttttaccgtt gctgcggaag tgctgcaaat gacctaacag aaaattagaa 73920 acatcttgct taatcgccgc gttattgcta cgaatgttgt tagccagtgc gttaaaacca 73980 gactctttta ccagattaaa aattttagcc atgatattca cctttcaatt gtaagggcac 74040 tatgccccgc tctctatgag ctttttaagt cgccattgac ttatcaatat ccactcattg 74100 ttaagtaatt attgataagt ggggcggtct tatcagaagt taaccttgcc gcccctttat 74160 ctgtgatgtt tcatctggta tctttaatta gtcgctataa acgaagccga cctgatagcg 74220 atgatcaatc atcacaataa ggcagtgcgt ccgactctaa ttagttactt tattgtaacc 74280 gtcggcaata tcgtctactt tgctttcgct ctcgcctatc cttttatact aactctcaat 74340 cgctaaccat cgcccactac gggaggttat tgcgaaagag tcattagccg tctaatattc 74400 cgacgtttta tatctttcgg caagtttact acataacccg actatgtact ttcatcttat 74460 gatgaatctc aagccgttca catcgtaaac gttcacacta aaccttattg ttaaatagca 74520 atttgccact ttttaaccac tccccgccgc tctcaattga gcgttaattc gtcggacttg 74580 cctaccctga ttaacttctt tcactgtagc accatgttag cgatggccgc cgctaccctt 74640 aaccgtgggc gattagctta agcctttgcg tgtactcaat gaatccgttt atcagtggtg 74700 acttttccag taagggaatg taaaactgtt taccctgata ttttagggag ttttacgccg 74760 ttttatctgg tatgctgtcc gatggttatt atcttccagc atatttaacc gatataggag 74820 aatgtttaaa ttgttaaaga gcggtaaatc tatcaacgtg ctttctcacg ttatgcaact 74880 gttagccgtt gttacgcttt tcactgaagc ggtgatttgc tcaagtgatg ctcattatgg 74940 accatgttgt ttttgccgtc aatcactttt ttaatctttt tcgtcgttcc cgctattggg 75000 aggatatatc aacccgcttt attgggttga agattaagcg atgaggctta acccatgatt 75060 catattgtta aagagcgttt agccttttat agtgtctgct aatcactaca tcattaaagg 75120 cttacttgtt cattaacggg gcgcctaagc ccgaactacc cgctgcacta tgtgccacct 75180 ttcccgcgat acgttgattt gggaactagt taacgttgtt ttgttaactt gtggttagta 75240 tggactacct taaccacgtt tgcaacctct tttttatcat cttcttttat tcttttccct 75300 tatctcgcgt gaatctatgc gaaagggcaa gatgataagt ctgatttgtc ttttaggaat 75360 ctagtgcgct ttcaagcatt gaccatttga aagtatcgaa caaatgaggc ttattttgtt 75420 gctcatcgtc cgcatgttca cgtatgaatc aggaatcaat tctagtggtc aactaagcgc 75480 cttatagtaa tgacgttccc gccacctgta agacaaatca tatttttaaa gagcgggatt 75540 tttcaagtca tcgctgacta tgctcactat atatcaggct ttttccggtg tcaatactct 75600 ttttcaagta ttttcacttt ctagcctgat ttttaaagag cggggcaatt tcttgccttg 75660 tatgttttac atcgtacccg atgaaatgaa gtatacatta atcagatgag gttgcaatag 75720 ggtaaagcaa attattttaa actttttcac tatagggagg taaaagagcg ggcgcacgtg 75780 atacatcata ggaaacggct tgtaaagcga taaaaagcgg cctgaaacgc actgtagcga 75840 gctaaaaggg agtaaggtat acaatcacct tagaaaagaa aaaagcctca tagcgaggct 75900 tataagaagg gtttaaaaca tcagatagat tatgaccata ggcatgacca cggcacaata 75960 aagaaggaag atcagtccta tggatttaag cattacccat agccgatagt aaaacaccca 76020 gcaggagaac gaccagaaaa aaccctacaa ccgttttaat gatgaattta ataaaccaca 76080 tagccataaa cctctttaat taaaggatgt aagaataaag catatcagcg acgaataacg 76140 ccactgtaac gacgaataag caccgccaaa aataatcatt ccccatttat atcaccctca 76200 atcagcccat gatttagcag gttagcaagt gataatccac cgtcaaaata tatagaacga 76260 aaatcatcgc gggtaatttc aaaatcagca aaggctagcc aatctttaag aggctgatat 76320 tccatcctgc aaaaagtgat aacaggtaac gttatctgac catcaagtgt gttaaacatg 76380 atagattgct cgccaacaaa acaagaaaag ccgtttgata atttaacttc tttaatagcc 76440 attatttata ctccaaaaaa tagattattt acttactagc cttaatgcag ttggctaaat 76500 taacgtgaac ggtataaaca gcgttatcag cgttattata aacggtagtc gcaagcattg 76560 attcgttcat cgggccataa ctgaccagat tcgtgacagc tttcgcatgg ctgtaacgtt 76620 gtacagtgcc aaatgtaact agttcaggat cgttatcagt acgcttgaga actagatcac 76680 tgttaggctg attgtctagt gttttggtac taacgaccag accatcacgg gaaacgttac 76740 tgaatttaac ttttaacacg atttgattgg tgtcactatc cgcaccgtta caaatcagcc 76800 aacctttatt gatggaacta gcgaaaacgt cagcggcatt agttgcagga gcaggactag 76860 cgatagcctt atcagaaaga gaagttacca gtgtactaac accgatcgcg gcgactagcg 76920 cataacttgc cagattaata gcctgcatta ctttcgccat ttttgttact ccttaggtag 76980 aatcaattaa ctattgccaa ctatataata aaaggaaaga gcgacaacta tttattttta 77040 gctttcttat aactcactga atcttaaaga aatttaattt actaacttct ctcattcagg 77100 gcatagctta ctacttctat tgttacttta taacataacg ccagaccgac ggggagaacg 77160 tgccgcataa acagacctac ggggcaacgg tacaacgtaa ccagcacggg ggaaggataa 77220 acgggtaacc tataatgtat gttgttacat gatgctattg ttagcggctt gcggggataa 77280 tataccaatc aaataattaa cccatcaaac tatca 77315

Claims (6)

  1. 서열번호 1로 표시되는, 대장균에 특이적 사멸능을 갖는 균주기탁번호 (KCTC18269P)의 박테리오파지 ECBP1.
  2. 제1항의 박테리오파지를 유효성분으로 포함하는 대장균에 의해 유발되는 닭 대장균증의 예방 또는 치료용 항균조성물.
  3. 제2항에 있어서,
    상기 박테리오파지의 유효농도는 104 ~ 1012 PFU/㎖ 인 것을 특징으로 하는 닭 대장균증의 예방 또는 치료용 항균조성물.
  4. 삭제
  5. 제1항의 박테리오파지를 유효성분으로 포함하는 닭 대장균증의 예방 또는 치료용 조성물.
  6. 제1항의 박테리오파지를 유효성분으로 포함하는 닭 대장균증의 예방 또는 치료용 사료첨가제 또는 음수 또는 분무를 통해 공급하는 액제 또는 분말제제 또는 동결건조제제.
KR1020140019121A 2014-02-19 2014-02-19 닭 대장균 예방 및 치료를 위한 신규 박테리오파지 및 이를 이용한 항균조성물 KR102125235B1 (ko)

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