CN113166770A - 重组大肠杆菌系统及其构建方法和其在合成α-1,2-岩藻糖基化寡糖中的应用 - Google Patents

重组大肠杆菌系统及其构建方法和其在合成α-1,2-岩藻糖基化寡糖中的应用 Download PDF

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CN113166770A
CN113166770A CN201980006916.9A CN201980006916A CN113166770A CN 113166770 A CN113166770 A CN 113166770A CN 201980006916 A CN201980006916 A CN 201980006916A CN 113166770 A CN113166770 A CN 113166770A
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gdp
alpha
gene
fucosyltransferase
fucose pyrophosphorylase
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黄锦
薛晓舟
李玉
秦慧民
佟毅
李义
陈博
李凡
安泰
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Cofco Nutrition and Health Research Institute Co Ltd
Jilin COFCO Bio Chemical Co Ltd
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Cofco Nutrition and Health Research Institute Co Ltd
Jilin COFCO Bio Chemical Co Ltd
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Abstract

涉及基因工程技术领域，提供了重组大肠杆菌系统及其构建方法和其在合成α‑1,2‑岩藻糖基化寡糖中的应用。该重组大肠杆菌系统具有α‑1,2‑岩藻糖基转移酶基因和GDP‑岩藻糖焦磷酸化酶基因。通过将α‑1,2‑岩藻糖基转移酶基因和GDP‑岩藻糖焦磷酸化酶基因转化到大肠杆菌中，构建基因工程菌，以表达α‑1,2‑岩藻糖基转移酶和GDP‑岩藻糖焦磷酸化酶，并将表达的α‑1,2‑岩藻糖基转移酶和GDP‑岩藻糖焦磷酸化酶用于酶法合成α‑1,2‑岩藻糖基化寡糖的。

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PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201980006916.9A 2019-11-15 2019-11-26 重组大肠杆菌系统及其构建方法和其在合成α-1,2-岩藻糖基化寡糖中的应用 Pending CN113166770A (zh)

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CN2019111195059 2019-11-15
CN201911119505 2019-11-15
PCT/CN2019/120873 WO2021093022A1 (zh) 2019-11-15 2019-11-26 重组大肠杆菌系统及其构建方法和其在合成α-1,2-岩藻糖基化寡糖中的应用

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CN113166770A true CN113166770A (zh) 2021-07-23

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Cited By (1)

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CN115466707A (zh) * 2022-06-27 2022-12-13 天津科技大学 一种生产2′-岩藻糖基乳糖的乳酸乳球菌及其应用

Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
CN113337554A (zh) * 2021-05-28 2021-09-03 华东理工大学 一种体外多酶级联催化合成岩藻糖基化乳糖的方法
CN113789341B (zh) * 2021-09-16 2022-10-11 陕西理工大学 BmSPI38的同型串联多聚体及其构建方法和应用

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KR101648352B1 (ko) * 2015-11-09 2016-08-16 서울대학교산학협력단 푸코실락토오스 생산용 재조합 대장균 및 이를 이용한 푸코실락토오스의 생산방법
CN108611384A (zh) * 2012-08-20 2018-10-02 中央研究院 寡糖的大规模酶合成的方法
CN109554385A (zh) * 2018-07-24 2019-04-02 石家庄葛兰德生物科技有限公司 一种基因工程菌制备2-岩藻糖基乳糖的方法

Patent Citations (3)

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CN108611384A (zh) * 2012-08-20 2018-10-02 中央研究院 寡糖的大规模酶合成的方法
KR101648352B1 (ko) * 2015-11-09 2016-08-16 서울대학교산학협력단 푸코실락토오스 생산용 재조합 대장균 및 이를 이용한 푸코실락토오스의 생산방법
CN109554385A (zh) * 2018-07-24 2019-04-02 石家庄葛兰德生物科技有限公司 一种基因工程菌制备2-岩藻糖基乳糖的方法

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115466707A (zh) * 2022-06-27 2022-12-13 天津科技大学 一种生产2′-岩藻糖基乳糖的乳酸乳球菌及其应用

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