CN113080000B - Method for improving florescence regulation and control of paphiopedilum callosum double-flower rate - Google Patents

Method for improving florescence regulation and control of paphiopedilum callosum double-flower rate Download PDF

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CN113080000B
CN113080000B CN202110378693.8A CN202110378693A CN113080000B CN 113080000 B CN113080000 B CN 113080000B CN 202110378693 A CN202110378693 A CN 202110378693A CN 113080000 B CN113080000 B CN 113080000B
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paphiopedilum
callosum
flowering
water
rate
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CN113080000A (en
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曾宋君
尹玉莹
吴坤林
房林
李琳
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South China Botanical Garden of CAS
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South China Botanical Garden of CAS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/60—Flowers; Ornamental plants
    • A01G22/63—Orchids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25—Greenhouse technology, e.g. cooling systems therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a method for improving callusA method for regulating and controlling the rate and the flowering period of paphiopedilum bisulture comprises the following steps of injecting gibberellin solution into the base of paphiopedilum callosum plants with 4-5 leaves before flowering. The invention adopts Gibberellin (GA) on the basis of improving the cultivation technology 3 ) The injection method can greatly improve the double-flower rate of the paphiopedilum callosum, the flowering phase is also advanced by 1-2 months, and the flowering rate and the flowering regularity are improved. The method has no report of regulating and controlling the paphiopedilum florescence by adopting a gibberellin injection method at home and abroad, can fill the blank at home and abroad, and has a positive promoting effect on promoting the development of the paphiopedilum industry in China. The technology is feasible and has high application value.

Description

Method for improving florescence regulation and control of paphiopedilum callosum double-flower rate
Technical Field
The invention belongs to the technical field of plant culture, and particularly relates to a method for improving the florescence regulation and control of a paphiopedilum callosum double-flower rate.
Background
Paphiopedilum, also called luodiaolan, xian pedunculan, etc., is a general name of Paphiopedilum (Paphiopedilum) belonging to Paphiopedilum of the family orchidaceae. Paphiopedilum has extremely high ornamental value due to unique flower shape, gorgeous flower color and lasting ornamental florescence, is popular in the market and is a very rare medium-high grade flower in the international flower market. However, paphiopedilum is one of the most endangered plant species in the world due to the destruction of ecological environment and excessive mining, and all wild species are listed in International trade convention on endangered wild animal and plant species (CITES) appendix I and are prohibited from trading. In recent years, the paphiopedilum industry in China develops rapidly, but the development of the paphiopedilum industry is restricted due to the reasons of small number of flowers, inconsistent flowering periods, low flowering rate and the like.
The native southeast Asia regions such as Vietnam and Thailand of Paphiopedilum callorum (Paphiodilum callosum) are one of Paphiopedilum plants which have high ornamental value and are widely cultivated in original Paphiopedilum species, and are also important parents of Paphiopedilum variegatum, wherein in a natural state, although the inflorescence has two flower primordia, the Paphiopedilum has 1 flower in most cases, the other flower degenerates, and the flowering phase is mostly spring before summer.
The invention content is as follows:
the invention aims to overcome the defects in the prior art and provides a method for improving the florescence regulation and control of paphiopedilum callosum, which comprises the following steps: gibberellin solutions were injected at the base of paphiopedilum callosum plants with 4-5 leaves before flowering.
The concentration of the gibberellin solution is preferably 25-75 mg/L.
The injection is specifically as follows: the injection is administered once every two weeks for 5-6 times, and each plant is administered with 0.3-0.5 ml.
The start time of the injection is preferably 8-9 months.
Preferably, the cultivation and management of the paphiopedilum callosum plant: the method comprises the steps of hardening seedlings of paphiopedilum callorum aseptic seeding, transplanting the paphiopedilum callorum aseptic seeding in a water curtain greenhouse, wherein a culture substrate is water moss, natural illumination and temperature are adopted, the water curtain greenhouse is designed to be 25 ℃, a fan is started, the water curtain is started in running water at 27 ℃, the temperature is 10-32 ℃ all the year round, the relative air humidity is 70-90%, watering and moisturizing are carried out, fertilization is started 1 month after transplanting, 5-10 Kangduo slow-release fertilizers (about 0.5 g for each 10 grains) are applied to each pot, more than 1 No. 3000-adulterated liquid is sprayed once a week, pot changing cultivation is carried out after 6-7 months, fertilization is started 1 month after pot changing, more than 10-20 Kangduo slow-release fertilizers (about 0.5 g for each 10 grains) are applied to each pot every half year, more than 1 No. 3000-adulterated liquid is sprayed once a week, and flowering again, and more than 2-2000-3500-adulterated liquid is sprayed once a week after planting 1 year.
The invention adopts Gibberellin (GA) on the basis of improving the cultivation technology 3 ) The injection method can greatly improve the double-flower rate of the paphiopedilum callosum, the flowering period is also advanced by 1-2 months, and the flowering rate and the flowering uniformity are improved. The method has no report of regulating and controlling the paphiopedilum florescence by adopting a gibberellin injection method at home and abroad, can fill the blank at home and abroad, and has a positive promoting effect on promoting the development of the paphiopedilum industry in China. The technology is feasible and has high application value.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the following description is given with reference to specific examples, but the present invention is not limited thereto.
Example 1
1. Cultivation and management of paphiopedilum callosum: sterile seeding seedlings with the height of 5-6 cm of Paphiopedilum callorum are planted by adopting Israel imported water moss when the seedlings are taken out of bottles in 9-10 months, and a planting vessel is a 1.7-inch white plastic cup. During planting, placing a paphiopedilum callosum sterile seeding culture bottle in a greenhouse for hardening seedlings for 2 weeks, taking out the paphiopedilum callosum sterile seeding culture bottle, cleaning an attached culture medium, wrapping roots of the paphiopedilum callosum sterile seeding seedlings by using water moss which is soaked in water for more than 24 hours and squeezed to dry, planting the paphiopedilum callosum sterile seeding culture bottle in a 1.7-inch white plastic cup, placing the paphiopedilum in a water curtain greenhouse for cultivation, naturally irradiating and heating, starting a fan when the water curtain greenhouse is designed to be 25 ℃, starting water flow of the water curtain when the temperature is 27 ℃, starting the water curtain at 10-32 ℃ all the year, and ensuring the relative humidity of air to be 70-90%. During cultivation management, watering is carried out for moisture preservation, and after 1 month, the survival rate can reach more than 95%. Fertilizing is started 1 month after transplanting, 5 Kangduo (nitrogen, phosphorus and potassium are 14-12-14) slow-release fertilizers with fertilizer preservation for 180 days are applied to each pot, 4000 times of liquid of No. 1 general fertilizers (nitrogen, phosphorus and potassium are 20-20-20) with water-soluble balance fertilizers is sprayed once a week until the leaves drip.
After 7 months, i.e., 5 months in the next year, 2.5 inch white plastic cups are used for pot change cultivation. Fertilizing is started 1 month after changing the pot, 10 Kangduo (14-12-14 of nitrogen, phosphorus and potassium) slow-release fertilizers with fertilizer conservation for 180 days are applied to the pot every half year, 4000 times of liquid of No. 1 universal fertilizer (20-20-20 of nitrogen, phosphorus and potassium) with water-soluble balance fertilizer flower majority is sprayed once a week until the leaf surfaces drip water. After planting for 1 year again, i.e. spraying 2000 times of No. 2 fertilizers (nitrogen, phosphorus and potassium are 10-30-20) for more flowers once a week from 5 months of the next year until flowers bloom, and spraying the fertilizers to the leaf surfaces for each time until water drops.
2. Gibberellin injection treatment and experimental results for paphiopedilum callosum: in 9 months, mature plants of vigorous growth of paphiopedilum callosum with 4-5 leaves were selected for Gibberellin (GA) 3 ) Solution injection treatment, 30 strains each were treated with no gibberellin solution as a control. When in injection, a 1ml injector is adopted to inject the plant base part, GA 3 The solution concentration is 25mg/L, 0.3ml is injected into each plant, and the injection is performed once every two weeks for 6 times continuously. Tong (Chinese character of 'tong')After the injection treatment, the flowering rate is 95% and the double-blossom rate is 75% in the next 3 months of the flowering phase, while the control without injection has the flowering rate of 3-4 months, the flowering rate of 80% and the double-blossom rate of 3%.
Example 2
1. Cultivation and management of paphiopedilum callosum: sterile sown seedlings of Paphiopedilum callosum (Paphiopedilum callosum) 6-7 cm in height are planted by adopting Israel imported water moss when the seedlings are delivered from bottles in 9 months, and a planting container is a 1.7-inch white plastic cup. During planting, placing a sterile seeding culture bottle of paphiopedilum callosum in a warm shed for hardening seedlings for 1 week, taking the sterile seeding of paphiopedilum callosum out of the culture bottle, cleaning an attached culture medium, wrapping the root of the sterile seeding of paphiopedilum callosum by using water moss which is soaked for more than 24 hours by using water and squeezed to dry, planting the sterile seeding in a 1.7-inch white plastic cup, placing the white plastic cup in a water curtain greenhouse for planting, and culturing under natural illumination and temperature, wherein when the water curtain greenhouse is designed to be 25 ℃, a fan is started, and when the temperature is 27 ℃, water curtain running water is started, the temperature is 10-32 ℃ all year round, and the relative humidity of air is 70-90%. During cultivation management, watering is carried out for moisture preservation, and after 1 month, the survival rate can reach more than 95%. Fertilizing is started 1 month after transplanting, 8 Kangduo (nitrogen, phosphorus and potassium are 14-12-14) slow-release fertilizers with fertilizer preservation for 180 days are applied to each pot, 3500 times of liquid of water-soluble balance No. 1 general fertilizers (nitrogen, phosphorus and potassium are 20-20-20) is sprayed once a week until water drops on leaf surfaces.
After 6 months, namely 3 months next year, 2.5 inches of white plastic cups are adopted for pot change cultivation. Fertilizing is started 1 month after changing the pot, 10 Kangduo (nitrogen, phosphorus and potassium are 14-12-14) slow release fertilizers with fertilizer conservation for 180 days are applied to the pot every half year, 3500 times of liquid of No. 1 general purpose fertilizers (nitrogen, phosphorus and potassium are 20-20-20) with water-soluble balance fertilizers is sprayed once a week until the foliage drips. After planting for 1 year, 3500 times of liquid of No. 2 fertilizers (with nitrogen, phosphorus and potassium of 10-30-20) which have more flowers are sprayed once every week from 3 months in the next year until flowers bloom and the leaves drip water every time.
2. Gibberellin injection treatment and experimental results for paphiopedilum callosum: selecting vigorous growing paphiopedilum callus plant with 4-5 leaves for Gibberellin (GA) at 8-9 months 3 ) Solution injection treatment, 30 strains each were treated with no gibberellin solution as a control. At the time of injection, the blood is collectedPlant base, GA, was injected with a 1ml syringe 3 The solution concentration is 50mg/L, 0.4ml is injected into each plant, and the injection is performed once every two weeks for 6 times continuously. After the treatment by injection, the flowering period of 3 months next in flowering period is 98%, and the double-blossom rate is 80%, while the control without injection is 3-4 months in flowering period, 82%, and 3%.
Example 3
1. Cultivation and management of paphiopedilum callosum: sterile seedlings of Paphiopedilum callosum (Paphiopedilum callosum) with the height of 5-8 cm are planted by adopting Israel imported water moor when the seedlings are taken out of bottles in 10 months, and a planting container is a white plastic cup with the size of 1.7 inches. During planting, after the sterile seeding culture bottle of paphiopedilum is placed in a warm shed and is hardened for 1 week, the sterile seeding of paphiopedilum is taken out from the culture bottle, an attached culture medium is cleaned, the roots of the sterile seeding of paphiopedilum are wrapped by water moss which is soaked for more than 24 hours by water and squeezed to dry, the sterile seeding of paphiopedilum is planted in a 1.7-inch white plastic cup and is placed in a water curtain greenhouse for planting, the natural illumination and the temperature are realized, the water curtain greenhouse is designed to be 25 ℃, a fan is started, when the temperature is 27 ℃, the water curtain flowing water is started, the temperature is 10-32 ℃ all year round, and the relative humidity of air is 70-90%. During cultivation management, watering is carried out for moisture preservation, and after 1 month, the survival rate can reach more than 95%. Fertilizing is started 1 month after transplanting, 10 slow release fertilizers with fertilizer conservation of Kangduo (nitrogen, phosphorus and potassium are 14-12-14) are applied to each pot for 180 days, 3000 times of a water-soluble balance fertilizer No. 1 general fertilizer (nitrogen, phosphorus and potassium are 20-20-20) is sprayed once a week until water drops on leaf surfaces.
After 6 months, i.e., 3 months in the next year, 2.5 inch white plastic cups are used for pot change cultivation. Fertilizing is started 1 month after the pots are changed, 20 good Kangduo (nitrogen, phosphorus and potassium are 14-12-14) slow-release fertilizers with fertilizer preservation capacity for 180 days are applied to the pots every half year, 3000 times of a water-soluble balance fertilizer flower No. 1 general fertilizer (nitrogen, phosphorus and potassium are 20-20-20) is sprayed once a week until the leaves drip. After planting for 1 year again, 3000 times of fertilizer No. 2 (nitrogen, phosphorus and potassium are 10-30-20) for more flowers is sprayed once a week from 3 months of the next year until flowers bloom, and the fertilizer is sprayed to the leaf surfaces every time until water drops.
2. Gibberellin injection treatment and experimental results for paphiopedilum callosum: selecting vigorous generation of paphiopedilum callosum with 4-5 leaves in 8 monthsCarrying out Gibberellin (GA) 3 ) Solution injection treatment, 30 strains each were treated with no gibberellin solution as a control. When injecting, the plant base part, GA, is injected by adopting a 1ml injector 3 The solution concentration is 75mg/L, 0.5ml is injected into each plant, and the injection is performed once every two weeks for 5 times continuously. After the treatment by injection, the flowering period is 2 months next, the flowering rate is 100%, and the double flowering rate is 85%, while the control without injection has the flowering period of 3-4 months, the flowering rate is 85%, and the double flowering rate is 5%.

Claims (2)

1. A method for improving the florescence regulation and control of the double-blossom rate of paphiopedilum callosum is characterized by comprising the following steps: injecting gibberellin solution into the base of the paphiopedilum callosum plant with 4-5 leaves before flowering;
the concentration of the gibberellin solution is 25-75 mg/L;
the injection is specifically as follows: injecting once every two weeks, continuously injecting for 5-6 times, and injecting 0.3-0.5ml per plant;
the start time of the injection is 8-9 months.
2. The method according to claim 1, wherein the cultivation and management of paphiopedilum callosum plants: the method comprises the steps of hardening seedlings of paphiopedilum callorum aseptic seeding, transplanting the paphiopedilum callorum aseptic seeding in a water curtain greenhouse, using water moss as a culture medium, naturally illuminating and maintaining the temperature, starting a fan when the water curtain greenhouse is designed to be 25 ℃, starting the water curtain running water when the temperature is 27 ℃, keeping the temperature at 10-32 ℃ all year round, keeping the relative air humidity at 70-90%, watering and moisturizing, starting fertilizing 1 month after transplanting, applying 5-10 granules of Kangduo slow-release fertilizer to each pot, spraying 1 # 3000-.
CN202110378693.8A 2021-04-08 2021-04-08 Method for improving florescence regulation and control of paphiopedilum callosum double-flower rate Active CN113080000B (en)

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CN102124876B (en) * 2010-11-10 2012-06-27 天津滨海国际花卉科技园区股份有限公司 Method for adjusting and controlling flowering phase of dendrobium nobile
CN102197768B (en) * 2011-05-18 2013-01-09 中国科学院昆明植物研究所 Method for adjusting flowering period of paphiopedilum micranthum
CN106472231B (en) * 2016-10-20 2020-04-21 中国科学院华南植物园 Method for introducing and domesticating paphiopedilum armeniacum in south China
CN106489483A (en) * 2016-10-20 2017-03-15 中国科学院华南植物园 A kind of method of Helen's pocket orchid in situ conservation

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