CN102197768B - Method for adjusting flowering period of paphiopedilum micranthum - Google Patents
Method for adjusting flowering period of paphiopedilum micranthum Download PDFInfo
- Publication number
- CN102197768B CN102197768B CN 201110129170 CN201110129170A CN102197768B CN 102197768 B CN102197768 B CN 102197768B CN 201110129170 CN201110129170 CN 201110129170 CN 201110129170 A CN201110129170 A CN 201110129170A CN 102197768 B CN102197768 B CN 102197768B
- Authority
- CN
- China
- Prior art keywords
- paphiopedilum micranthum
- flowering
- paphiopedilum
- micranthum
- adjusting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000519045 Paphiopedilum micranthum Species 0.000 title claims abstract description 46
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 29
- 241000196324 Embryophyta Species 0.000 claims abstract description 18
- PVRBGBGMDLPYKG-UHFFFAOYSA-N 6-benzyl-7h-purine Chemical compound N=1C=NC=2N=CNC=2C=1CC1=CC=CC=C1 PVRBGBGMDLPYKG-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 12
- 239000003448 gibberellin Substances 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims description 19
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 2
- 239000005556 hormone Substances 0.000 abstract description 6
- 229940088597 hormone Drugs 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 abstract 2
- 230000012010 growth Effects 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 241000519406 Paphiopedilum Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000003375 plant hormone Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 241001553178 Arachis glabrata Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241001164374 Calyx Species 0.000 description 1
- 241001516485 Cypripedium Species 0.000 description 1
- 235000019738 Limestone Nutrition 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 241000358848 Orchidantha maxillarioides Species 0.000 description 1
- 241000519419 Paphiopedilum armeniacum Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015709 bud dormancy process Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000006028 limestone Substances 0.000 description 1
- 239000003226 mitogen Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
Images
Landscapes
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for adjusting flowering period of paphiopedilum micranthum. The method for adjusting flowering period of paphiopedilum micranthum comprises the steps of treating a grown plant of paphiopedilum micranthum with gibberellins GA3 and 6-benzylpurine 6-BA of different concentrations; when the gibberellins GA3 is used for treatment, the concentrations are respectively 100mgL-1, 200mgL-1 and 400mgL-1; and when the 6-benzylpurine 6-BA is used for treatment, the concentrations are respectively 100mgL-1, 200mgL-1 and 400mgL-1. According to the market demand, different hormones of different concentrations are used for adjusting the flowering rate, the flowering time, the flowering period and the length of scape, and the method speeds up flowering of the paphiopedilum micranthum by 54 to 70 days. The flowering period adjusting method of paphiopedilum micranthum disclosed by the invention is characterized by high operability, obvious control effect and the like, can provide operable technical support for commercial flower production of paphiopedilum micranthum.
Description
Technical field
The invention belongs to biological technical field, particularly, relate to a kind of method of adjusting flowering period of paphiopedilum micranthum.
Background technology
Paphiopedilum micranthum (
Paphiopedilum micranthum) be the orchid family Paphiopedilum (
Paphiopedilum) plant, concentrate ground and the North Vietnam such as the Yunnan be distributed in China, Guizhou, Guangxi, be born in the In Limestone Area of height above sea level 400～1700m and shrub in ground or the sylvan life rock crevice; Have several at the most bar horizontal walk walk stem, diameter 1.5-2.5mm, 3-5 piece of leaf, spend gorgeous greatly, scape 10-30cm, the dark cryptomere of lip, ellipticity is spherical.The characteristics such as Paphiopedilum micranthum has colored shape peculiar, and pattern is gorgeous, and the florescence is long, have very high ornamental value and cultivation value, be called as " silver-colored pocket " or " teenage girl " (Ban Xiuzhi, 2009) at gardening circle, with paphiopedilum armeniacum and be called " golden boy and jade girl ", it is the treasure in the orchid.
In recent years, owing to the demand of market to cypripedium increases day by day, carry out Paphiopedilum micranthum industrialization cultivation production and will become the important channel of meeting the need of market.Flowers consumption habit according to China, the consumption of potted flower mainly concentrates on the major holidays such as New Year's Day, the Spring Festival, the natural florescence of Paphiopedilum micranthum is generally in the 3-5 month, therefore, take effective artificial measures to come the florescence of Paphiopedilum micranthum is carried out Artificial Control, make flowering time be advanced to New Year's Day, Spring Festival, will become one of key link in the production of Paphiopedilum micranthum commercialization standard.
In the research of florescence control, the opening that the utilization of plant exogenous hormone has demonstrated multiple ornamental flower has facilitation effect, in the prior art there are no the report of the method for adjusting flowering period of paphiopedilum micranthum.
Summary of the invention
The object of the present invention is to provide a kind of method of adjusting flowering period of paphiopedilum micranthum, utilize the gibberellin (GA of variable concentrations
3) and 6-benzyl purine (6-BA) respectively the Paphiopedilum micranthum plant that grows up is processed, with advance flowering period 54-70 days of Paphiopedilum micranthum, flowering rate can reach 93%, for fitly providing Paphiopedilum micranthum commodity potted flower that the florescence control key technology of easy operating is provided in batches at New Year's Day, Spring Festival.
In order to realize above-mentioned purpose of the present invention, the invention provides following technical scheme:
The method of adjusting flowering period of paphiopedilum micranthum is with Paphiopedilum micranthum
Paphiopedilum micranthumAdult plant is material, utilizes the gibberellin GA of variable concentrations
3Respectively the adult plant of Paphiopedilum micranthum is processed with 6-benzyl purine 6-BA, described gibberellin is processed and is adopted GA
3100mg L
-, GA
3200mg L
-1, GA
3400mg L
-1Described 6-benzyl purine 6-BA processes and adopts 6-BA100mg L
-1, BA200mg L
-1, BA400mg L
-1
Compared with prior art, excellent benefit of the present invention is:
The present invention with Paphiopedilum micranthum (
Paphiopedilum micranthum) the healthy adult plant be material, utilize the gibberellin GA of variable concentrations
3Respectively the adult plant of Paphiopedilum micranthum is sprayed processing with 6-benzyl purine 6-BA, gibberellin is processed and is adopted GA
3100mg L
-, GA
3200mg L
-1, GA
3400mg L
-16-benzyl purine 6-BA processes and adopts 6-BA100mg L
-1, BA200mg L
-1, BA400mg L
-1Studied hormone and concentration flowering rate, the scape of Paphiopedilum micranthum stretched out 3cm to the impact of the time of blooming, florescence and flowering quality.The result shows, the 6-BA of variable concentrations, GA
3Process and all can be shifted to an earlier date 54-70 days the flowering stage of Paphiopedilum micranthum; GA
3The time of blooming after the processing significantly processes in advance than 6-BA, GA200 mg L
-1Processing the shortest is 131 ± 27 days; But 6-BA processes from flowering rate and the neat degree of blooming, BA100 mg L
-1The flowering rate of processing is up to 93%, and flowering time only front and back differed 2-5 days; From the florescence, BA400 mg L
-1Process and GA100 mg L
-All but kind of blooming period prolonging is 3 days; From other proterties of blooming, the scape length that gibberellin is processed significantly is longer than the processing of contrast and the basic element of cell division.The present invention can be according to market demand, regulate flowering rate, flowering time, florescence and the scape length of Paphiopedilum micranthum with the variable concentrations of hormon, the advantages such as its method has strong operability, and regulating effect is remarkable, the commodity peanut that can be Paphiopedilum micranthum produces exercisable technical support is provided.Can when production application, suitably regulate and process hormone and concentration level according to the product demand time, so that the Paphiopedilum micranthum commodity that meet time needs flower to be provided.
Description of drawings:
Fig. 1 is that hormon is processed the impact on flowering rate.
Embodiment:
The below further specifies essentiality content of the present invention with embodiments of the invention, but does not limit the present invention with this.
Embodiment 1:
1, materials and methods:
1-1 test material and design:
Choose 112 strain Paphiopedilum micranthum healthy adult plant, cultivating in leaf mould, queen closer and bark ratio is in the cultivation matrix of 1:1:1, the daily mean temperature in cultivation greenhouse is 18 ℃, plant is carried out the processing of hormon variable concentrations, the control group fresh water spraying, totally 7 kinds of processing are processed 16 strains (seeing Table 1) for every kind
Measure with ruler that the blade of every strain plant is long and blade is wide, and Calculate Leaf Area (leaf area=blade length * blade wide * leaf-area coefficient 0.684), according to the leaf area of plant with 0.2L/m
2The amount of spraying spray on the blade of Paphiopedilum micranthum the plant hormone of above-mentioned concentration is disposable with micro-sprayer, the time of spraying is on September 3rd, 2009.
1-2 Ce Liang ﹑ observes and statistical analysis:
Record every strain plant processing afterwards to the time that scape stretches out 3cm, scape stretches out 3cm to the time of flowering, and flowering is long to the time of withering and scape, lip diameter, the flower shape facilities such as size of calyx sheet.Adopt at last the SPSS13.0 statistical analysis software to carry out data analysis.
2, results and analysis:
The 2-1 hormon is processed the impact on Paphiopedilum micranthum plant blossom rate:
Hormon concentration is processed the impact different (Fig. 1) on the Paphiopedilum micranthum flowering rate, according to can finding out of adding up among the figure, compares with control group, and the flowering rate of 6-BA processed group is respectively 6-BA100mg L
-1: 93%, 6-BA200mg L
-1: 88%, 6-BA400mg L
-1: 88%; And GA
3The flowering rate of 3 concentration of processed group is along with the increase of concentration, and flowering rate is lower, is respectively GA
3100mg L
-: 88%, GA
3200mg L
-1: 69%, GA
3400mg L
-1: 44%; The flowering rate of control group is 81%.Can be found out by above result, the flowering rate of 6-BA processed group is significantly higher than control group, and GA
3Processed group is (100 mg.L when concentration is low
-1) higher (χ of flowering rate
2=41.111, P＜0.001)
The 2-2 hormon is processed the impact on flowering period of paphiopedilum micranthum:
As can be seen from Table 2, all HORMONE TREATMENT plant begin all to have shifted to an earlier date 54～70 days than control group is remarkable to the time of blooming from processing, wherein GA
3Approximately processed 45 days in advance than 6-BA to taking out the required time of Roripa 3cm after processing, but it takes out Roripa 3cm and significantly be longer than the 6-BA processing to the time of blooming, and the concentration of processing is higher, required time is longer, even is longer than control treatment; And 6-BA compares GA to the time of blooming after processing
3Processing approximately prolongs 10-15 days, but its flowering rate and the regularity of blooming all are better than GA
3Process, the flowering time that 6-BA processes only differed 2-5 days, and the flowering time that GA processes then differed 15-27 days; 6-BA processes and reaches 23 ± 2 days at 400 mg/L lower florescences of concentration, compares photograph (CK) significant prolongation 3 days, and gibberellin is processed under 100mg/L concentration, and the florescence also can reach 23 ± 5 days, and other concentration are processed does not have significant difference compared with the control.
The 2-3 hormon is processed the bloom impact of proterties of Paphiopedilum micranthum:
After the length of the length of the scape length of the Paphiopedilum micranthum of different disposal, flower diameter, left and right sides sepal and wide, middle sepal and the statistical analysis relatively such as wide, as can be seen from Table 3, compared with the control, scape length has significant difference, wherein, and GA
3Process that the scape of (100mg/L, 200mg/L and 400mg/L) is long to be respectively 17.2,18.9 and 21.3cm, significantly be longer than 14.8,14.6 and 13.7cm that 6-BA processes (100mg/L, 200mg/L and 400mg/L), control treatment is 14.8cm, and processing with 6-BA does not have significant difference.The lip diameter of all processing does not all have significant difference, GA
3The side sepal size of processing is slightly larger than 6-BA and processes.
3, technical scheme:
Plant hormone has important effect in growth and development of plants, many test card clear-cells mitogens can be induced the orchid flower bud differentiation, can promote cell division, inducing cell enlarges, remove the apical dominance of plant growth, promote lateral bud to form and growth, reduce chlorophyllous decomposition, postpone leaf senile.The Main Function of gibberellin is the longitudinal growth that promotes cell, thereby plays an important role in the elongation of stem.Can cause the elongation of internode of whole strain plant, impel the biennial plant Blooming, break the dormancy of seed and other organs, stratification; Suppress ripe, the lateral bud dormancy, aging, stem tuber forms.
To sum up, the present invention grows up plant culture in daily mean temperature is 18 ℃ cultivation greenhouse with Paphiopedilum micranthum, and cultivation matrix is leaf mould, queen closer and bark=1:1:1, uses the GA of variable concentrations
3It is processed September with 6-BA, can promote advance flowering period, but GA
3The florescence of processing more shifts to an earlier date, and early the time (such as New Year's Day) can be utilized GA for flower if need
3(200 mg L
-1) Paphiopedilum micranthum processed to reach need the flower requirement; If for taking time evening (such as the Spring Festival), can utilize 6-BA(400 mg L
-1) the Paphiopedilum micranthum plant is processed, to reach higher flowering rate (88%) and open neat degree, obtain simultaneously the long flowering time (23 ± 2d).The present invention can suitably regulate when production application and process hormone and concentration level according to the product demand time, so that the Paphiopedilum micranthum commodity that meet time needs flower to be provided.
Claims (1)
1. the method for adjusting flowering period of paphiopedilum micranthum is with Paphiopedilum micranthum
Paphiopedilum micranthumAdult plant is material, optional gibberellin GA
3Spray with the adult plant of its a pair of Paphiopedilum micranthum of 6-benzyl purine 6-BA, a concentration is only adopted in a processing, and described gibberellin is processed and adopted GA
3100mgL
-1Or GA
3200mgL
-1Or GA
3400mgL
-1Described 6-benzyl purine 6-BA processes and adopts 6-BA100mgL
-1Or 6-BA200mgL
-1Or 6-BA400mgL
-1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110129170 CN102197768B (en) | 2011-05-18 | 2011-05-18 | Method for adjusting flowering period of paphiopedilum micranthum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110129170 CN102197768B (en) | 2011-05-18 | 2011-05-18 | Method for adjusting flowering period of paphiopedilum micranthum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102197768A CN102197768A (en) | 2011-09-28 |
| CN102197768B true CN102197768B (en) | 2013-01-09 |
Family
ID=44658960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110129170 Active CN102197768B (en) | 2011-05-18 | 2011-05-18 | Method for adjusting flowering period of paphiopedilum micranthum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102197768B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102440155B (en) * | 2011-10-17 | 2012-10-31 | 东莞市农业种子研究所 | Method for regulating and controlling the blooming period of paphiopedilum |
| CN102726225B (en) * | 2012-05-25 | 2013-09-11 | 北京林大林业科技股份有限公司 | Florescence control method for cymbidium hybridum |
| CN103749174B (en) * | 2013-12-31 | 2015-09-23 | 泉州市泉美生物科技有限公司 | Spathiphyllum kochii florescence control method |
| CN105284366B (en) * | 2015-10-30 | 2018-01-26 | 华南农业大学 | A kind of method for shortening pocket orchid fruit development period and improving seed germination rate |
| CN105850626A (en) * | 2016-03-25 | 2016-08-17 | 镇远县醉美果业有限公司 | Flowering-phase management method for honey peaches |
| CN111279917B (en) * | 2020-04-03 | 2022-05-13 | 潍坊科技学院 | Flowering phase regulation and application method of male sterile line of wild shallots and cultivated shallots |
| CN113080000B (en) * | 2021-04-08 | 2022-09-02 | 中国科学院华南植物园 | Method for improving florescence regulation and control of paphiopedilum callosum double-flower rate |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1711844A (en) * | 2005-07-15 | 2005-12-28 | 中国科学院长春应用化学研究所 | Plant auxin composition for advancing flowering period of kaffir lily |
| CN1957668A (en) * | 2005-11-02 | 2007-05-09 | 中国林业科学研究院林业研究所 | Method for regulating and controlling blooming period of spring dendrobium stem |
| CN101015253A (en) * | 2007-02-16 | 2007-08-15 | 北京林业大学 | Florescence combined regulation and control method of macranthus Hui orchid |
| CN101627690A (en) * | 2009-08-05 | 2010-01-20 | 中国科学院昆明植物研究所 | Method for regulating and controlling anthesis of paphiopedilum armeniacum |
| CH700447A1 (en) * | 2009-02-23 | 2010-08-31 | Helmut Weber | Stable, upright stand for e.g. orchid, has plug-in sleeve attached to base of pot, where sleeve plunges rod, orchid fastened with massive blooms, and intermediate layer rotated in direction of new bloom drive |
-
2011
- 2011-05-18 CN CN 201110129170 patent/CN102197768B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1711844A (en) * | 2005-07-15 | 2005-12-28 | 中国科学院长春应用化学研究所 | Plant auxin composition for advancing flowering period of kaffir lily |
| CN1957668A (en) * | 2005-11-02 | 2007-05-09 | 中国林业科学研究院林业研究所 | Method for regulating and controlling blooming period of spring dendrobium stem |
| CN101015253A (en) * | 2007-02-16 | 2007-08-15 | 北京林业大学 | Florescence combined regulation and control method of macranthus Hui orchid |
| CH700447A1 (en) * | 2009-02-23 | 2010-08-31 | Helmut Weber | Stable, upright stand for e.g. orchid, has plug-in sleeve attached to base of pot, where sleeve plunges rod, orchid fastened with massive blooms, and intermediate layer rotated in direction of new bloom drive |
| CN101627690A (en) * | 2009-08-05 | 2010-01-20 | 中国科学院昆明植物研究所 | Method for regulating and controlling anthesis of paphiopedilum armeniacum |
Non-Patent Citations (2)
| Title |
|---|
| 曾宋君等.国产兜兰属植物的引种和栽培.《中国野生植物资源》.2010,第29卷(第2期),53-58. * |
| 黄建等.不同激素处理对蝴蝶兰开花的影响.《浙江农业科学》.2009,(第3期),493-394、499. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102197768A (en) | 2011-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102197768B (en) | Method for adjusting flowering period of paphiopedilum micranthum | |
| CN103651121B (en) | A kind of bletilla differentiation, strong seedling culture base | |
| CN100998298B (en) | Method for accelerating the advancing blossom of big-bract shell dendrobe by utilizing plant growth regulator | |
| CN102224789B (en) | Flowering method of peony in autumn | |
| CN102511326B (en) | Method for stunting potted osmanthus fragrans | |
| CN104206226B (en) | A kind of liquor-saturated gold tree-like breeding method of preserved fragrant grape high quality fruit | |
| CN102598984B (en) | Cultivation method for promoting pot-cultured tree peony to blossom on National Day | |
| CN102972435B (en) | Stem dormant bud germination accelerating agent and germination accelerating method thereof | |
| CN109924074A (en) | A method of south grape off-season production is improved into flower quality | |
| CN103988780B (en) | A kind of method of direct induction Moth orchid indefinite bud | |
| CN103098639A (en) | Bulbil type konjac rapid propagation and seed production method | |
| CN103609318B (en) | Method for regulating chrysanthemum flowering phase | |
| CN106171983A (en) | A kind of cultural method of sword-leaved cymbidium test tube flowering | |
| CN106818476B (en) | The efficient quick propagation method of natural Triploid Lacquer Tree | |
| CN102550257A (en) | Method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulator | |
| CN105123373B (en) | Improve the method for cultivating seedlings of Cenxi switch oil tea afforestation drought-resistant ability | |
| CN115500167B (en) | Method for inducing butterfly orchid pedicel to germinate and bloom in advance by using triiodobenzoic acid | |
| CN103609351A (en) | Method for regulating chrysanthemum flowering phase | |
| CN106359085A (en) | E.urophylla*E.saligna rooting culture medium and application thereof | |
| CN103650811A (en) | Regulation and control method for three-dimensional-picking tea garden flower-less branch picking crown layers | |
| CN104871954B (en) | A kind of breezing and producing method of red Chinese toon pollination liquid and red Chinese toon seed | |
| CN107410005A (en) | A kind of spinach cenospecies and its selection | |
| CN103125364A (en) | Introduction and domestication propagation method and application of ottelia acuminate | |
| CN105075833A (en) | Method for stereo-cultivation of shade-tolerant flowers on live stems of big elaeis guineensis trees | |
| CN111528007A (en) | Application of kinetin in treatment of industrial hemp plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| OL01 | Intention to license declared |