CN105284366B - A kind of method for shortening pocket orchid fruit development period and improving seed germination rate - Google Patents

A kind of method for shortening pocket orchid fruit development period and improving seed germination rate Download PDF

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Publication number
CN105284366B
CN105284366B CN201510731703.6A CN201510731703A CN105284366B CN 105284366 B CN105284366 B CN 105284366B CN 201510731703 A CN201510731703 A CN 201510731703A CN 105284366 B CN105284366 B CN 105284366B
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China
Prior art keywords
pocket orchid
pocket
shortening
germination rate
seed germination
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CN201510731703.6A
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CN105284366A (en
Inventor
谭剑锋
刘伟
刘运权
王燕君
韩航
韩一航
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Guangzhou Scau Biotech Development Co Ltd
South China Agricultural University
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Guangzhou Scau Biotech Development Co Ltd
South China Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

Abstract

The invention belongs to field of plant cultivation, and in particular to a kind of method for shortening pocket orchid fruit development period and improving seed germination rate.The described method for shortening pocket orchid fruit development period and improving seed germination rate, is comprised the following steps：(1) when the pocket orchid plant flower by pollination comes off, the wound formed when flower comes off is closed with quick lime；(2) when the ovary of treated pocket orchid plant in step (1) starts to expand, 12~36h of developmental pocket orchid ovary is handled with 6 BA solution.The present invention handles ovary with and by 6 BA by the protection to fruit and shortens its puberty, reduces influence of the summer high temperature to pocket orchid fruit development, makes seed have embryo rate and harvest fruit ratio to significantly improve.

Description

A kind of method for shortening pocket orchid fruit development period and improving seed germination rate
Technical field
The invention belongs to field of plant cultivation, and in particular to one kind shortens pocket orchid fruit development period and improves seed germination rate Method.
Background technology
Pocket orchid (Paphiopedilum Pfitzer) refers to orchid family cypripedium, is distributed mainly on subspecies torrid areas extremely Pacific Islands, it is grown under tropical rain forest, ground is raw or grows nonparasitically upon another plant.Except evidence《Chinese Plants will》Middle record originates in 18 of China Kind, it is found that many pocket orchid species or mutation for originating in China again in succession in recent years, is distributed in the southern torrid zone with subtropical zone south edge Area and western part, it is more with Guangxi province, Guizhou Province, Yunnan Province's distribution.
Pocket orchid type is unique, and plant type is graceful, enjoys favor.China's cypripedium aboundresources, but protect and rationally open Hair utilizes and still has the problem of more.Existing more than the 100 years history of the blue artificial cultivation of pocket, and select quantity crowd More Hybrids.But in global orchid in the market, although fan is numerous, the blue yield of pocket is not high all the time, and restricts pocket Seed germination rate is low in the bottleneck of blue industry development, exactly seedling large-scale production and the problems such as stability difference.
The in vitro non-symbiosis germination of seed, it is the pocket orchid species seedling production technology generally used in current global range.The skill The implementation of art, it is that seed germination rate is low and stability is poor the problem of two maximums, which not only improves production cost, also limit The controllability of high-volume seeling industry.The maturity for generally believing pocket orchid fruit at present be influence germination rate it is mostly important because Plain (Zeng Songjun etc., 2007), and difficult sprout (Huang Wei graceful etc., 2010) of more last mature seed of full maturity seed.Pocket orchid fruit Some specific period is arrived in real development, the germination rate highest of seed, and blue next for the pocket of not of the same race or kind, different niches Say, the most suitable time of seed development is different.For example, Philippine's pocket blue (P.philipinense), paphiopedilum armeniacum, purple Germination rate highest when 90 days after point pocket blue (P.godefroye) pollination (Lee et al., 1999；Lee,2007；Juanjuan ZHANG etc., 2013)；Chrysanthemum pocket orchid (P.primulinum) most suitable period is 110 days (Lee, 2007) after pollination；Huge valve pocket is blue (P.bellatulum) most suitable period is 130 days after pollination.In addition, although seed amount is many in pocket orchid fruit, but fruit The factors such as the high temperature of growth course, seeds abortion can be caused, form embryoless seeds, can also significantly affect germination rate.
Most pockets blue florescence needs 3 months or even longer time in spring, the development of its fruit, such as in the market Most common kind evil spirit Supreme Being pocket is blue, the germination rate highest of its fruit seed at 180~220 days after pollination, and fruit development mistake Journey is just meeting the high temperature of summer, causes seed to have the decline of embryo rate, the increase of decayed fruit rate, these are all to influence pocket orchid species seedling industrialization production Problem.
The content of the invention
For overcome the deficiencies in the prior art and shortcoming, it is an object of the invention to provide one kind to shorten pocket orchid fruit development Phase and the method for improving seed germination rate, this method can effectively accelerate the development of pocket orchid fruit and seed, seed is faster reached To the stage suitably sprouted, summer high temperature is effectively reduced to fruit and the influence of seed development.
The purpose of the present invention is achieved by the following technical programs：
A kind of method for shortening pocket orchid fruit development period and improving seed germination rate, is comprised the following steps：
(1) when the pocket orchid plant flower by pollination comes off, the wound formed when flower comes off is closed with quick lime；
(2) when the ovary of treated pocket orchid plant in step (1) starts to expand, handled with 6-BA solution in development 12~36h of pocket orchid ovary；
Pocket orchid plant described in step (1) is preferably that robust plant, the sturdy straight elevators of Hua Jing, flower be big and flower pattern, color and luster Outstanding pocket orchid plant；
The blue preferably red magic Supreme Being's pocket of pocket described in step (1) is blue；
Pocket orchid plant described in step (1) should avoid getting wet after pollination before flower comes off, to prevent that flower is rotten It is rotten and cause fruit rot；
Pollination described in step (1) is preferably artificial pollination；
6-BA solution described in step (2) preferably comprises 1% (v/v) Tween-80 and final concentration of 25~100mg/L 6-BA；
(v/v) Tween-80 of 6-BA solution further preferably 1% and final concentration of 50mg/L described in step (2) 6-BA；
Described in step (2) 6-BA solution processing ovary operation be preferably：First had begun to absorbent cotton parcel swollen Big ovary, 6-BA solution is equably dropped on absorbent cotton, absorbent cotton is impregnated with 6-BA solution, after handling 12~36h hours Remove absorbent cotton；
The time of processing described in step (2) is preferably 24h；
Pocket orchid plant described in step (2) after pollination 120 days or so can fruit picking sowing, with traditional method 180 Compare within~220 days, this method can shift to an earlier date fruit picking in 2~3 months, avoid shadow of the high temperature season to pocket orchid fruit development of summer Ring；
The present invention is had the following advantages relative to prior art and effect：
(1) present invention is simple to operate, and cost is low, and 120 days or so i.e. after pollination for the pocket orchid plant after 6-BA is handled Can fruit picking sowing, compared with traditional method 180~220 days, this method can shift to an earlier date fruit picking in 2~3 months, and just avoid summer High temperature season.
(2) present invention shortens its puberty by the protection to fruit and using the ovary of 6-BA processing pocket orchid plant, makes The period that pocket orchid species is suitably sprouted significantly shifts to an earlier date, and the fruit handled through this method, has embryo rate significantly to raise, fruit harvest rate shows Write and improve, the germination rate of seed significantly improves.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
6-BA is 6- benzyls aminoadenine (6-Benzylaminopurine), molecular formula C12H11N5, molecular weight is 225.26, CAS accession number are 1214-39-7；
Embodiment 1
A kind of method for shortening pocket orchid fruit development period and improving seed germination rate, comprises the following steps：
(1) in red magic Supreme Being's pocket orchid Post flowering selection robust plant, the sturdy straight elevators of Hua Jing, flower is big and flower pattern, color and luster are outstanding Plant, carry out artificial pollination, and avoid getting wet before flower comes off；After flower comes off, i.e., come off with quick lime closing flower When the wound that is formed；
(2) when the ovary of treated pocket orchid plant in step (1) starts to expand, first opened with absorbent cotton parcel Begin the ovary expanded, with quantitative dropper by 6-BA solution (6-BA containing final concentration of 25mg/L and 1% (v/v) tween- 80) equably drop on absorbent cotton, processing removes absorbent cotton after 24 hours；
(3) 120 days or so after pollination can fruit picking sowing.
Wherein, in the fruit that the present embodiment obtains, it is 60.96% that seed, which has embryo rate, and after planting seed germination rate is 34.87%.
Embodiment 2
A kind of method for shortening pocket orchid fruit development period and improving seed germination rate, comprises the following steps：
(1) in red magic Supreme Being's pocket orchid Post flowering selection robust plant, the sturdy straight elevators of Hua Jing, flower is big and flower pattern, color and luster are outstanding Plant, carry out artificial pollination, and avoid getting wet before flower comes off；After flower comes off, i.e., come off with quick lime closing flower When the wound that is formed；
(2) when the ovary of treated pocket orchid plant in step (1) starts to expand, first opened with absorbent cotton parcel Begin the ovary expanded, with quantitative dropper by 6-BA solution (6-BA containing final concentration of 50mg/L and 1% (v/v) tween- 80) equably drop on absorbent cotton, processing removes absorbent cotton after 24 hours.
(3) 120 days or so after pollination can fruit picking sowing.
Wherein, in the fruit that the present embodiment obtains, it is 71.27% that seed, which has embryo rate, and after planting seed germination rate is 42.18%.
Embodiment 3
A kind of method for shortening pocket orchid fruit development period and improving seed germination rate, comprises the following steps：
(1) in red magic Supreme Being's pocket orchid Post flowering selection robust plant, the sturdy straight elevators of Hua Jing, flower is big and flower pattern, color and luster are outstanding Plant, carry out artificial pollination, and avoid getting wet before flower comes off.After flower comes off, i.e., come off with quick lime closing flower When the wound that is formed；
(2) when the ovary of treated pocket orchid plant in step (1) starts to expand, first opened with absorbent cotton parcel Begin the ovary expanded, with quantitative dropper by 6-BA solution (6-BA containing final concentration of 100mg/L and 1% (v/v) tween- 80) equably drop on absorbent cotton, processing removes absorbent cotton after 24 hours；
(3) 120 days or so after pollination can fruit picking sowing；
Wherein, in the fruit that the present embodiment obtains, it is 58.47% that seed, which has embryo rate, and after planting seed germination rate is 49.71%.
Comparative example 1
A kind of method for shortening pocket orchid fruit development period and improving seed germination rate, comprises the following steps：
(1) in red magic Supreme Being's pocket orchid Post flowering selection robust plant, the sturdy straight elevators of Hua Jing, flower is big and flower pattern, color and luster are outstanding Plant, carry out artificial pollination, and avoid getting wet before flower comes off.After flower comes off, i.e., come off with quick lime closing flower When the wound that is formed；
(2) when the ovary of treated pocket orchid plant in step (1) starts to expand, first opened with absorbent cotton parcel Begin the ovary expanded, and is equably dropped in the solution containing 1% (v/v) Tween-80 on absorbent cotton with quantitative dropper, after 24 hours Remove absorbent cotton.
(3) 180~220 days after pollination can fruit picking sowing.
Wherein, in the fruit that the present embodiment obtains, it is 43.45% that seed, which has embryo rate, and after planting seed germination rate is 13.87%.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification, Equivalent substitute mode is should be, is included within protection scope of the present invention.

Claims (7)

  1. A kind of 1. method for shortening pocket orchid fruit development period and improving seed germination rate, it is characterised in that comprise the following steps：
    (1) when the pocket orchid plant flower by pollination comes off, the wound formed when flower comes off is closed with quick lime；
    (2) when the ovary of treated pocket orchid plant in step (1) starts to expand, pocket orchid ovary 12 is handled with 6-BA solution ~36h；
    6-BA solution described in step (2) contains 1%v/v Tween-80 and final concentration of 25~100mg/L 6-BA；
    Described in step (2) 6-BA solution processing ovary operation be：The ovary expanded first is had begun to absorbent cotton parcel, 6-BA solution is equably dropped on absorbent cotton, absorbent cotton is impregnated with 6-BA solution, degreasing is removed after handling 12~36h hours Cotton.
  2. 2. the method according to claim 1 for shortening pocket orchid fruit development period and improving seed germination rate, it is characterised in that：
    The pocket that pocket orchid plant described in step (1) is robust plant, the sturdy straight elevators of Hua Jing, flower are big and flower pattern, color and luster are outstanding Blue plant.
  3. 3. the method according to claim 1 for shortening pocket orchid fruit development period and improving seed germination rate, it is characterised in that：
    Pocket described in step (1) is blue for red magic Supreme Being's pocket orchid.
  4. 4. the method according to claim 1 for shortening pocket orchid fruit development period and improving seed germination rate, it is characterised in that：
    Pocket orchid plant described in step (1) should avoid getting wet after pollination before flower comes off, to prevent flower from rotting Cause fruit rot.
  5. 5. the method according to claim 1 for shortening pocket orchid fruit development period and improving seed germination rate, it is characterised in that：
    Pollination described in step (1) is artificial pollination.
  6. 6. the method according to claim 1 for shortening pocket orchid fruit development period and improving seed germination rate, it is characterised in that：
    6-BA solution described in step (2) contains 1%v/v Tween-80s and final concentration of 50mg/L 6-BA.
  7. 7. the method according to claim 1 for shortening pocket orchid fruit development period and improving seed germination rate, it is characterised in that：
    The time of described processing is 24h.
CN201510731703.6A 2015-10-30 2015-10-30 A kind of method for shortening pocket orchid fruit development period and improving seed germination rate Expired - Fee Related CN105284366B (en)

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CN106386488B (en) * 2016-09-09 2018-12-21 山东省烟台市农业科学研究院 It is a kind of to improve big flower pattern pocket orchid seed germination rate and its cultural method
CN109287487B (en) * 2016-09-09 2022-05-27 山东省烟台市农业科学研究院 Seed germination rate improving method and cultivation method for paphiopedilum makino
CN107114029A (en) * 2017-04-07 2017-09-01 扬州大学 A kind of method of quickly breaking dormancy of birch-leaf pear seeds
CN110149950B (en) * 2019-06-06 2021-12-03 广西壮族自治区中国科学院广西植物研究所 Method for improving development quality of paphiopedilum albolanum seeds and test solution thereof
CN113170626B (en) * 2021-04-30 2022-05-24 江苏里下河地区农业科学研究所 Method for promoting germination of cymbidium goeringii seeds

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CN1150820C (en) * 2001-04-25 2004-05-26 四川龙蟒福生科技有限责任公司 Plant growth regulating composition with dwarfing, ripe-ness promoting and resisting functions
CN102197768B (en) * 2011-05-18 2013-01-09 中国科学院昆明植物研究所 Method for adjusting flowering period of paphiopedilum micranthum
CN103621369B (en) * 2013-10-27 2015-07-08 大连东芳果菜专业合作社 Technical method for shortening flower cultivation period in greenhouse cultivation of stone fruit trees

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