CN106386488B - It is a kind of to improve big flower pattern pocket orchid seed germination rate and its cultural method - Google Patents
It is a kind of to improve big flower pattern pocket orchid seed germination rate and its cultural method Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Cultivation Of Plants (AREA)
Abstract
Big flower pattern pocket orchid seed germination rate and its cultural method are improved the present invention relates to a kind of.More specifically, the present invention provides a kind of method for improving big flower pattern pocket orchid seed germination rate, this method comprises the following steps: (1) carrying out artificial pollination in big flower pattern pocket orchid Post flowering；(2) it 180 days to 240 days acquisition seeds and is seeded on seed germination medium after pollination, to obtain sprout by seed.The present invention also provides a kind of cultural method of big flower pattern pocket orchid, the cultural method improves seed germination rate using the method for above-mentioned raising germination rate.Production flower diameter can have using the method for the present invention and be greater than 12cm, even up to 15 to 16cm big flower pattern pocket is blue, and inflorescence is complete, petal is intact, and appreciation effect is good, and it is high to sow germination rate, the tissue-cultured seedling speed of growth is fast, and survival rate can shorten to cultivation period 3 years up to 85% or more.
Description
Technical field
The invention belongs to flower cultures and horticultural field, are related to the breeding of pocket orchid and cultural method, more specifically, the present invention
It is related to the breeding cultivation method of the especially big flower pattern pocket orchid of pocket orchid.
Background technique
Pocket orchid is in great demand in International Flower market, while being also an Endangered taxa of orchid family.Apricot yellow, the Paphiopedilum micranthum in China
Zeng Yong obtains world orchid exhibition whole audience main champion, obtains more or less a hundred U.S. orchid and opens up awards.Blue a total of 79 wild species of pocket, wherein about 1/
3 originate from China, are the endangered species of wild fauna and flora species uniquely protected by international trade pact (CITES) in orchid family.China's pocket is blue
Flowers market demand gap is big, and price is prohibitively expensive, and 100 to 150 yuan of price of common kind single plant, rare kind single plant price can
Up to thousands of members.2012, China produced the only tens of thousands of strains of pocket orchid per year, and pocket orchid yield increases 10 times in recent years, and annual product is not all for answering
It asks.
However, pocket orchid is also an Endangered taxa of orchid family although pocket orchid is in great demand in International Flower market.Pocket orchid species
Son does not have endosperm, needs to sprout with mycosymbiosis in the natural environment, and germination rate is extremely low, and pocket orchid tissue cultures are states
The bottleneck of border pocket orchid industry development, there are proliferation rates it is low, browning is serious the problems such as.Pocket orchid is commercialized volume production technology in the world at present
It does not set up still, breeds still using aseptic seeding as main means, but there is germination rates extremely low, the low problem of planting percent, and not
Of the same race and kind pocket orchid seed collecting time phase difference is totally different, and most suitable kinds of culture medium is also different.In addition, pocket orchid facility cultivation is deposited
In poor quality, the disadvantage that growth cycle is long, the speed of growth is slow, even 4 to 5 years are needed from bottle seedling to blooming.
The pocket orchid of China mainland lacks the pocket orchid species of independent intellectual property right at present, and seedling depends on import Taiwan,
More lack the cultural method and reproduction technique of science.Pocket orchid tissue cultures are the bottlenecks of international pocket orchid industry development, there is proliferation
The problems such as rate is low, browning is serious.China's pocket orchid science research input is obviously insufficient at present, is primarily present following several respects problem.China
Research about pocket orchid focuses primarily upon initial species conserving culture, such as paphiopedilum armeniacum, Paphiopedilum micranthum, band leaf pocket is blue, huge valve pocket is blue,
Homochromy pocket orchid, mountain of papers pocket orchid etc., the blue most several flower diameter of pocket are less than 10cm, and the commercial variety about Hua Jing greater than 12cm is ground
Study carefully less.However, compared with the small flower pattern of initial species or multi-pattern type pocket orchid, although the blue ornamental value of the big flower pattern pocket of commodity is wanted
It is much higher, but this kind of pocket orchid is cenospecies, ploidy is complicated, often can only obtain seldom seed, it is difficult to large-scale production benefit
With, and seed germination rate flower pattern smaller than initial species or multi-pattern type pocket orchid are lower.
In particular, although the meat pie series pocket in big flower pattern pocket orchid is blue with relatively high ornamental value and with higher
Cross compatibility, but since its hybrid seed is incomplete without endosperm or embryonic development, germination rate is extremely low and former in its natural state
Bulb melting brown rate is higher, so that seed germination rate, planting percent and quality problem are especially prominent.
Therefore how with rapid breeding, breeding and the big flower pattern pocket orchid of cultivating high quality such flower plant scientific research is had become
The emphasis of project has far deep realistic meaning in its research and development.
Summary of the invention
The purpose of the present invention is studying the links of the big breeding of flower pattern pocket orchid and cultivation, to cultivate the high-quality pocket having
Orchid, and the pocket orchid speed of growth and yield rate are improved, it effectively reduces cost, promotes the market competitiveness, be the large-scale production of pocket orchid
Theoretical direction and technical support are provided.
For seed germination rate is low, browning is serious, planting percent is low, production is all present in big flower pattern pocket orchid in the prior art
The technical problems such as the phase is long, quality is low, the present invention provide a kind of side for improving big flower pattern pocket orchid seed germination rate in first aspect
Method, described method includes following steps: (1) carrying out artificial pollination in big flower pattern pocket orchid Post flowering；(2) 180 days extremely after pollination
240 days acquisition seeds are simultaneously seeded on seed germination medium, to obtain sprout by seed.
In some preferred embodiments of first aspect present invention, it is 1/ that acquired seed, which is sowed in formula,
The seed of 4MS+1mg/L 6-BA+0.1mg/L NAA+1g/L peptone+25g/L banana+5.5g/L agar+1g/L active carbon is sprouted
It sends out on culture medium.
The present invention additionally provides a kind of cultural method of big flower pattern pocket orchid in second aspect, and the method is using the present invention the
Method described in one side improves seed germination rate.
The seed germination rate of big flower pattern pocket orchid can be significantly improved using the method for the present invention, reduce or eliminate browning degree,
To provide technical support to expand growth scale and shortening production cycle.In some preferred implementations of second aspect of the present invention
In mode, also strict control Plant technique condition includes but is not limited to humidity, temperature, illumination and water and fertilizer condition, improves great Hua
The kind shoot survival percent of type pocket orchid improves the quality of big flower pattern pocket orchid product, to be further expansion growth scale and shortening
Production cycle provides technical support.
Detailed description of the invention
Fig. 1 is the photo of green meat pie series pocket orchid；
Fig. 2 is the photo of red meat cake series pocket orchid；
Fig. 3 is change curve of the meat pie class pocket orchid seed germination rate with the time after pollination；
Fig. 4 is tissue culture shoot survival percent of the meat pie class pocket orchid under different relative humidities；
Fig. 5 uses survival rate in the case where different substrates to change with time when being the orchid tissue culture transplantation of seedlings of meat pie class pocket；
Fig. 6 is to use the change of leaf growth total amount at any time in the case where different substrates when strong sprout changes basin in meat pie class pocket orchid
Change；
Fig. 7 a is the photo for the meat pie class pocket orchid potted flower product planted using contrast method；
Fig. 7 b is the photo for the meat pie class pocket orchid potted flower product planted using the method for the present invention.
Specific embodiment
As described above, the present invention provides a kind of method for improving big flower pattern pocket orchid seed germination rate in first aspect,
In some preferred embodiments, described method includes following steps:
(1) artificial pollination is carried out in big flower pattern pocket orchid Post flowering；With
(2) (such as 180,210,240 or 270 days) obtain seed and are seeded in seed 180 days to 270 days after pollination
On germination medium, to obtain sprout by seed.
The present inventor has found existing big flower pattern on the basis of furtheing investigate the crossbreeding and sowing of big flower pattern pocket orchid
The main reason for pocket orchid seed germination rate is low at least two.
The present invention by further investigation discovery, influence the most critical of the seed germination rate of the especially big flower pattern pocket orchid of pocket orchid because
Element is the maturity of the seed for sowing.The prior art although also selection have certain maturity sowing only sow,
It does not unify the understanding to the maturity of pocket orchid species for being suitable for sowing, some thinks to come after self-pollination 300 to 360 days abundant
The seed for expanding capsule is best, such as CN201510565855.3 discloses a kind of meat pie series pocket orchid crossbreeding and its seedling
Rapid propagation method, wherein using capsule is sufficiently expanded as seed in 300 to 360 days after pollination；Some, which thinks to be obtained from, awards
The seed of 140~150 days fruit pods is advisable after powder, for example, CN201410514229.7 disclose pocket orchid seed germination medium and
Cultural method, wherein using the seed for the fruit pod pollinated 140~150 days；Some thinks the fruit obtained from 110~120 days after pollination
More preferably, such as the seed collected using 110 to 120 days after pollination of CN201310742625.0 is for sowing for the seed of pod；Have
Think that summer high temperature can be encountered using the seed harvested at 180~220 days after pollination, so that seed is caused to have the decline of embryo rate, it is rotten
Fruit rate increases, it is proposed that discloses one kind using 120 days or so the seeds harvested after pollination, such as CN201510731703.6
The method for shortening pocket orchid fruit development period and improving seed germination rate, it is believed that magic Supreme Being's pocket orchid harvests at 180~220 days after pollination
Seed can encounter summer high temperature, so that seed be caused to have the decline of embryo rate, decayed fruit rate increases, it is therefore proposed that 120 days left sides after pollination
It the right side can fruit picking sowing.But the present inventor has been found that after study, the seed development stage of big flower pattern pocket orchid is to influence seed
The key factor sprouted after aseptic seeding, seed development initial stage, tender seed it is close it is raw on the placental tissue of fruit pod, it is difficult
To separate, the browning quickly being seeded on culture medium, only a small amount of seed has sprouting ability.Seed development excessively at
It is in sepia when ripe, dry to loosen, the germination rate of sowing is also very low, it may be possible to because kind skin is reached maturity, it is difficult to breaking dormancy,
Need ultrasonic broken skin or chemical substance treatment that could promote to sprout.It is blue for big flower pattern pocket, use harvesting in 120 days after pollination
Seed germination rate is very low, and some is even without sprouting, and there are breed differences for the kernel maturing of suitable sowing, for meat
For cake series pocket blue (referring to Fig. 1 and 2), the seed with harvesting in 240 days after pollination is best.
The present inventors have additionally discovered that it is blue relative to small flower pattern pocket, such as initial species pocket orchid, such as paphiopedilum armeniacum, Paphiopedilum micranthum, band
(the flower diameter of these pockets orchid are respectively less than 12cm, and ornamental value is poor) such as leaf pocket orchid, huge valve pocket orchid, homochromy pocket orchid, mountain of papers pocket orchids, quotient
Although the blue ornamental value of the big flower pattern pocket of product wants much higher, this kind of pocket orchid is cenospecies, and ploidy is complicated, can only often be obtained
To seldom seed, it is difficult to which large-scale production utilizes, and seed germination rate flower pattern smaller than initial species or multi-pattern type pocket are blue
It is lower.But the problems of big flower pattern pocket orchid in the prior art can largely be overcome using the method for the present invention.
Then, in some preferred embodiments, the big flower pattern pocket orchid is the pocket orchid species that flower is greater than 12cm, more preferably F1
For big flower pattern pocket orchid species, further preferably seed is sprouted, seedling survives, quality problem is more serious and the production cycle is long
Meat pie series pocket orchid species (Paphiopedilum Pacific Shamrock).
In some preferred embodiments, the seed be after pollination 210 days to 240 days obtain seed (such as
210,220,230 or 240), 240 days seeds obtained more preferably after pollination.
The present invention is not particularly limited Pollination Modes, but in some preferred embodiments, using manually awarding
The mode of powder, the artificial pollination can may be artificial hybridization pollination or both pollination sides for artificial pollination self
The combination of formula.But, in order to avoid trait segregation, more preferably artificial hybridization is pollinated.
In some preferred embodiments, the seed germination medium is 1/4MS+1mg/L 6-BA+0.1mg/L
NAA+1g/L peptone+25g/L banana+5.5g/L agar+1g/L active carbon.
The present invention has found that the composition of the seed germination medium of big flower pattern pocket orchid is the another of influence germination rate through overtesting
A key factor.There are many kinds of the basal mediums sprouted in the prior art for pocket orchid species, for example, MS, NDM, spend precious No. 1,
VW culture medium, wherein the component further added is even more multifarious.In addition, it is generally believed that containing a large amount of in banana pulp
Organic matters such as amino acid, the hormone and enzyme and more complicated natural complex of ingredient, the main component of peptone are amino acid, because
This is provided to provide various amino using the main purpose of banana puree and peptone in the seed germination medium of pocket orchid
Acid while does not both use under normal circumstances.But find after study, suitable 6- is added on the basis of 1/4MS
BA, NAA, agar and active carbon, and add suitable peptone simultaneously and banana muddy flesh can to significantly improve big flower pattern pocket blue
Seed germination rate.
The present invention additionally provides a kind of cultural method of big flower pattern pocket orchid in second aspect, and the method is using the present invention the
Method described in one side improves seed germination rate.
In some preferred embodiments, sowing 100 days to 150 days (such as 100,110,120,130,140 or
150 days) after, the sprout for having developed blade is transferred in strong seedling culture base and is cultivated to obtain tissue-cultured seedling, the strong seedling culture base
Formula be 1/4MS+4mg/L 6-BA+0.2mg/L NAA+25g/L banana+5.5g/L agar+1g/L active carbon.The present inventor
It finds through overtesting, can be grown well in the case where not adding any peptone in strong seedling culture base, thus real
The purpose of existing strong sprout.
In some preferred embodiments, when the plant height of the tissue-cultured seedling is 4cm to 6cm, tissue-cultured seedling is transferred to
After natural light lower refining seedling 20 days, tissue-cultured seedling is transplanted in the cultivating container equipped with bark matrix, and with the progress of following condition
Maintenance: relative humidities are 60% to 80%, more preferably 80%.If relative humidity is lower than 60%, it will to big flower pattern
The plant strain growth of pocket orchid causes unfavorable irreversible influence, it is therefore desirable to need strictly relative humidity after practicing transplantation of seedlings
Control is 60% or more.Temperature condition can be 18 DEG C to 28 DEG C of daytime, 15 DEG C to 18 DEG C of night；Illumination condition is 10000lux
To 15000lux；Water and fertilizer management condition is to sprinkle profoundly water within every 5 to 7 days once, and individually or alternately application N:P:K is 30:10:10
With the compound fertilizer of 20:20:20, extension rate is preferably 3000 times, and frequency of administration is that 2 to 4 weeks are primary, and method of application is poured for root zone
Filling and/or foliage-spray.
In some preferred embodiments, it carries out once changing basin when strain width is equal to or more than 9cm, and in strain width
Carried out when equal to or more than 22cm it is secondary change basin, change the matrix that basin uses to be the sphagna for being 1:1 by mass ratio and bark group
At, and conserved with following condition: relative humidities are 60% to 80%, more preferably 80%；Temperature condition is
18 DEG C to 28 DEG C of daytime, 15 DEG C to 18 DEG C of night；Illumination condition is 10000lux to 15000lux；Water and fertilizer management condition is every 7
The compound fertilizer primary, application N:P:K is 20:20:20 was sprinkled profoundly water to 10 days, extension rate is 3000 times, and frequency of administration is every 2 weeks
Once, method of application is root-zone irrigation and/or foliage-spray.
In some preferred embodiments, it is secondary change basin after and the progress flower forcing in 6 months before Target Flowering,
Conserved during flower forcing with following condition: relative humidities are not less than 50% more preferably 80%；Temperature condition is
25 DEG C to 27 DEG C of daytime, 16 DEG C to 18 DEG C of night；Illumination condition is 10000lux to 15000lux；Water and fertilizer management condition is every 7
The compound fertilizer primary, application N:P:K is 10:30:10 was sprinkled profoundly water to 10 days, extension rate is 3000 times, and frequency of administration was 4 Mondays
Secondary, method of application is root-zone irrigation and/or foliage-spray.
In some preferred embodiments, the bark matrix used when transplanting after white silk seedling is pine bark, preferably
Loose squama, the more preferably size of bark are 3mm to 7mm.In other preferred embodiment, the base that is used when changing basin
Bark contained in matter is pine bark, and preferably loose squama, the more preferably size of bark are 10mm to 20mm.
Practice seedling after transplant when, the culture medium that Baijunling dilution washes root can be used, cleaning while into
Row disinfection.
The present inventor also found that the survival rate of big flower pattern pocket orchid species seedling is low in the prior art and product quality is owed through overtesting
It is good also practice seedling with seedling bottle outlet after used culture substrate it is closely related, if use bark as matrix after practicing seedling and/or
Change after basin use the combination of sphagna and bark as matrix can significantly big flower pattern pocket orchid bottle seedling survival rate.
In a specific embodiment, the method for the present invention includes selection, breeding method, aseptic seeding and the group of kind
Culture, tissue-cultured seedling field planting are knitted with Screening matrix, the management of basin seedling and blooming period management technology etc., can specifically include following steps: (a)
Select the big flower pattern pocket orchid species with objective trait；(b) in pocket orchid species Post flowering, the plant of healthy mature is selected to carry out people
Work selfing or hybridization pollination；(c) seed in fruit pod is seeded on culture medium, strong sprout and culture of rootage is carried out after sprouting；(d)
Training tissue culture seedling, disinfection and field planting；(e) basin, light temperature, water and fertilizer management technology are changed；(f) low temperature flower forcing and florescence fertilizer and water management skill
Art.
In a more specific embodiment, the Huang of 3 years mature seedlings, flowers big (diameter is greater than 12cm) can choose
Color, green and red cultivar take the blue series of America pocket as the F1 generation new product of main parents breeding if meat pie series pocket is blue
Kind, it is broadly divided into safflower and green spends series.The pocket orchid species Post flowering of healthy mature is selected, artificial autocopulation is carried out or hybridization is educated
Kind.When pollination, remove the lip of Parent first, choose lower male parent flower powder with needle, be placed in maternal column cap, because pollen load has
Toughness substance, therefore, pollen grain are easy to be sticked on column cap.The fruit pod of selection pollination after ripening in 180 days to 270 days, production
Aseptic seeding culture medium is formulated as 1/4MS+1mg/L 6-BA+0.1mg/L NAA+1g/L peptone+25g/L banana+5.5g/L
Agar+1g/L active carbon.White seed in fruit pod is seeded on the culture medium made.After sowing 30 days to 40 days, kind
Son starts to sprout, and the dotted tissue of white occurs, and turns green, green bud point occurs.After sowing 100 days to 150 days, it can will develop
The sprout of blade is transferred to strong seedling culture base, is formulated as 1/4MS+4mg/L 6-BA+0.2mg/L NAA+25g/L banana+5.5g/L
Agar+1g/L active carbon.When plant height is 4cm to 6cm, tissue-cultured seedling band bottle is transferred to after being cultivated 20 days under natural light and is practiced
Seedling (the white silk seedling time is preferably selected to carry out in or so November), takes out plant, cleans root culture with 1000 times of dilutions of Baijunling
Base is transplanted in 1.7 cun of transparent nutritive cubes, and matrix is bark.Maintenance management condition: humidity is to determine tissue-cultured seedling transplanting survival rate
An important factor for, optimum humidity is 60 to 80%, when humidity reaches 60%, can the blue ground sprinkler nearby of potting pocket, be sprayed,
Cascade ventilation；18 DEG C to 28 DEG C, 15 DEG C to 18 DEG C of night of temperature condition daytime；Big flower pattern pocket orchid light control 10000lux extremely
15000lux can carry out light control in 75% shading net shading using obscurity.Water and fertilizer management condition: bark is base
Matter needs to pour for general 5 to 7 days primary (matrix 8 at dry can moisturizing), and watering will irrigate every time.Concentration of fertilizer is 3000 times, battalion
Health can apply fertilizer once every 2 weeks for a long time, and root zone can be poured using liquid fertilizer and sprays leaf surface.The nutrition of big flower pattern pocket orchid is raw
For a long time based on nitrogenous fertilizer and balanced fertilizer, commonly using N:P:K is 30:10:10 and/or 20:20:20 compound fertilizer.1 year, plant width will be conserved
1.5 cun of plant greater than 9cm change basin into 2.5 cun of transparent nutritive cubes, and after maintenance 1 year, 2.5 cun plant of the strain width greater than 22cm are changed
For basin into 3 cun of transparent nutritive cubes, matrix is sphagna: bark=1:1.Maintenance management condition: 18 DEG C to 28 DEG C of daytime, night 15
DEG C to 18 DEG C.When summer reaches 28 DEG C, can reinforce divulging information and take cooling measure, when winter is down to 15 DEG C, carry out heat preservation or
Heating；Relative humidity 50 to 80% when humidity is greater than 80%, can reinforce ventilation to promote air to flow, humidity is lower than 50%
When, it ground sprinkler, spraying, cascade can divulge information near potting pocket orchid；The intensity of illumination of big flower pattern pocket orchid may be controlled to
10000lux to 15000lux can use obscurity to carry out shading control for 75% shading net shading in the case of necessary
System.Water and fertilizer management condition: sphagna adds bark when being matrix, pours within general 7 to 10 days primary.Watering will irrigate every time, and rainy day is not
Watering.Concentration of fertilizer is 3000 times, and fertilising in vegetative growth phase 2 weeks is primary, and fertilising in reproductive stage 1 month is primary, can be used
Liquid fertilizer pours root zone and sprays leaf surface.Pocket orchid growth period based on nitrogenous fertilizer and balanced fertilizer, can specifically use N:P:K for 30:
10:10 and/or 20:20:20 compound fertilizer.The flower forcing date be Target Flowering before 6 months, day night temperature be controlled in 25 to 27 DEG C/
16 to 18 DEG C of progress flower forcing processing, intensity of illumination 10000lux to 15000lux.Florescence reduces fertilising, can be with fertilising one in every 4 weeks
It is secondary, P fertilizer is properly increased, can specifically use N:P:K for 10:30:10 compound fertilizer, root zone can be poured using liquid fertilizer and sprays leaf
Surface.
Can have following advantage etc. using method of the invention: 1, pocket orchid filial generation diameter is greater than 12cm, or even reaches
To 15 to 16cm.2, aseptic seeding germination rate is high, and the tissue-cultured seedling speed of growth is fast.3, bottle seedling matrix is bark, and meat pie series pocket is blue
Bottle seedling survival rate up to 85% or more.4, shorten cultivation period by 3 years.5, inflorescence is complete, and petal is intact, appreciation effect
It is good.
Embodiment
Following embodiment is used to further illustrate essentiality content of the invention.Technical solution and embodiment according to the present invention
Description, perhaps same domain technical staff on the basis of the present invention can also to technical solution of the present invention carry out it is some modification and
It improves.Therefore, the modification and improvement done on the basis of without departing from main technical schemes of the present invention should belong to the present invention and be wanted
Seek the range of protection.Bark is the loose squama of 5mm when the bark matrix used when transplanting after white silk seedling in following embodiment, and changes basin
When the bark matrix or matrix that use contained in bark matrix be 15mm pine squama.
Embodiment 1
The meat pie series pocket orchid species (referring to Fig. 1 left figure) of healthy mature are selected, artificial hybridization breeding is carried out.When pollination,
The lip for removing Parent first is chosen lower male parent flower powder with needle, is placed in maternal column cap, because pollen load has goo
Matter, therefore, pollen grain are easy to be sticked on column cap.The fruit pod of 30 days to 300 days different maturity of selection pollination, is sowed respectively
On seed germination medium, culture medium prescription is 1/4MS+1mg/L6-BA+0.1mg/L NAA+1g/L peptone+25g/L fragrant
Any of several broadleaf plants+5.5g/L agar+1g/L active carbon, and germination rate is counted after 60 days.
By the statistical result of Fig. 3 as it can be seen that the germination rate of meat pie series pocket orchid and seed maturity have direct relation.Pollination
Seed is not sprouted on 120 days inoculation mediums afterwards, 240 days seed germination rate highests after pollination, after pollination more than 300 days seeds because
It is excessively mature, it is difficult to sprout.Therefore, the selection sowing time has a major impact seed germination rate.
Embodiment 2
Using the tissue-cultured seedling of meat pie series pocket orchid species described in embodiment 1, after bottle outlet, cultivate in 1.7 cun of transparent nutrition
Alms bowl, matrix are bark.Different maintenance humidity management conditions are arranged: humidity is 60% and 80%.After culture 200 days, statistics is survived
Rate.For test result as shown in figure 4, meat pie pocket orchid resistance is poor, survival rate is low.When relative humidity is 80%, meat pie tissue-cultured seedling is survived
Rate is up to 79%.When relative humidity is 60%, meat pie tissue culture shoot survival percent is 51%.Therefore, relative humidity is more suitable for for 80%
The growth of tissue-cultured seedling bottle outlet.
Embodiment 3
Using meat pie series pocket orchid species described in embodiment 1, seed sprouting is carried out in the essentially identical method of embodiment 1
Rate test, the difference is that using following seed germination medium.As a result as follows.
As can be seen from the above table, using 1/4MS+1mg/L 6-BA+0.1mg/L NAA+1g/L peptone+25g/L banana+
5.5g/L agar+1g/L active carbon can significantly improve the seed germination rate of big flower pattern pocket orchid as seed germination medium.
Embodiment 4
Using meat pie series pocket orchid species used in embodiment 1 as test material, the tissue-cultured seedling band bottle of plant height 4 to 6cm is shifted
After cultivating 20 days under to natural light, plant is taken out, root culture medium is cleaned with 1000 times of dilutions of Baijunling, transplants in 1.7 cun
In transparent nutritive cube, matrix is respectively bark, zeolite: bark: coconut palm block=2:1:2, fruit tree branch, water plant and coconut palm block, respectively at 64
It, 167 days, 250 days, after 365 days count survival rate.
Maintenance management condition: humidity 60 to 80%；18 DEG C to 28 DEG C, 15 DEG C to 18 DEG C of night of temperature condition daytime；Illumination
10000lux to 15000lux carries out shading control in 75% shading net using obscurity.Water and fertilizer management condition: general 5 to 7
It is poured once, and matrix 8 just answers timely moisturizing at dry.Watering will irrigate every time, and rainy day does not water.Concentration of fertilizer is 3000 times,
Fertilising in vegetative growth phase 2 weeks is primary, pours root zone using liquid fertilizer and sprays leaf surface and alternately apply fertilizer, wherein vegetative growth phase is with nitrogen
Based on fertilizer and balanced fertilizer, specially alternately application N:P:K is 30:10:10 and 20:20:20 compound fertilizer.
As seen from Figure 5, pocket orchid tissue-cultured seedling based assays the result shows that, using bark as matrix survival rate highest, cultivate 6 months
Survival rate reaches 82%, followed by fruit tree branch and zeolite: bark: coconut palm block=2:1:1 afterwards, and survival rate is up to 72%, still, fruit tree branch
Matrix in the course of cultivation, easily causes fungi and bacterium infection, and fungoid lime color mustiness object occurs in getting up early, and bacterium occurs in the later period
Property the water stain shape object of grey will improve pocket orchid disease (such as stem rot) probability as matrix.
Embodiment 5
Using meat pie series pocket orchid species described in embodiment 1 as test material, 1.7 cun plant of the strain width greater than 9cm is selected to change basin
Into 2.5 cun of transparent nutritive cubes, matrix treatments are respectively sphagna, bark, sphagna: branch=2:1, sphagna: bark=1:1, water
Tongue fur: bark: sheep dung=1:1:1.
Maintenance management condition: 18 DEG C to 28 DEG C of daytime, 15 DEG C to 18 DEG C of night；Humidity 50 to 80%；Illumination requirement
10000lux to 15000lux controls illumination in 75% shading net shading using obscurity.Water and fertilizer management condition: every 7 days extremely
It sprinkles profoundly water within 10 days primary.Concentration of fertilizer is 3000 times, and fertilising in vegetative growth phase 2 weeks is primary, fertilising one in reproductive stage 1 month
It is secondary, root zone is poured using liquid fertilizer and sprays leaf surface combination fertilization mode, pocket orchid vegetative growth phase with nitrogenous fertilizer and it is balanced it is fertile based on,
The alternately compound fertilizer that application N:P:K is 30:10:10 and 20:20:20.
As seen from Figure 6, sphagna: when bark=1:1 is matrix, the leaf growth amount of meat pie is maximum, is grown by 4.9cm
It is the matrix species for being most suitable for seedlings growth in meat pie class for other matrix to 65.83cm.Secondly, sphagna: tree
Skin: sheep dung=1:1:1 increment is also larger, and addition organic matter is conducive to pocket orchid leaf growth.Both matrix are better than pure
Sphagna and bark, and the effect that bark is added in matrix is better than branch.
Embodiment 6
The mature seedling in 3 years of meat pie series pocket orchid species described in selection example 1.The pocket orchid plant of healthy mature is selected,
Artificial autocopulation's pollination is carried out in Post flowering.When pollination, remove the lip of Parent first, choose lower male parent flower powder with needle, places
In maternal column cap, because pollen load has stickum, therefore, pollen grain can be sticked on column cap.Selection pollination 120 days after at
Ripe fruit pod makes aseptic seeding culture medium, is formulated and lives for MS+1mg/L6-BA+0.1mg/L NAA+5.5g/L agar+1g/L
Property charcoal.Seed in fruit pod is seeded on the culture medium made.After sowing 30 to 40 days, seed starts to sprout, and occurs white
Color dot shape tissue, and turn green, there is green bud point, measures germination rate at this time.
After sowing 100 days to 150 days, the sprout for having developed blade can be transferred to strong seedling culture base, be formulated as MS+4mg/L
6-BA+0.2mg/L NAA+25g/L banana+5.5g/L agar+1g/L active carbon.
Band bottle, which is transferred under natural light cultivate, when will grow to plant height 4 in strong seedling culture base to 6cm carries out experienced seedling for 20 days.
After practicing seedling, plant is taken out, cleans root culture medium with 1000 times of dilutions of Baijunling, transplanting is saturating in 1.7 cun
In bright nutritive cube, matrix is sphagna.Maintenance management condition: humidity is 60 to 80%, attached in potting pocket orchid when humidity is lower than 60%
Nearly ground sprinkler, spraying, cascade ventilation；18 DEG C to 28 DEG C, 15 DEG C to 18 DEG C of night of temperature condition daytime；Intensity of illumination is controlled
For 10000lux to 15000lux, shading control is carried out in 75% shading net using obscurity.Water and fertilizer management condition: bark base
Matter 8 is at moisturizing when dry (generally require and pour for 5 to 7 days once).Watering will irrigate (rainy day does not water) every time.Concentration of fertilizer is
3000 times, fertilising in vegetative growth phase 2 weeks is primary, pours the fertilization mode that root zone combination sprays leaf surface using liquid fertilizer.Pocket orchid battalion
For a long time based on nitrogenous fertilizer and balanced fertilizer, specifically used N:P:K is the compound fertilizer of 20:20:20 for health.
1.5 cun plant of 1 year, plant width greater than 9cm will be conserved and change basin into 2.5 cun of transparent nutritive cubes, after maintenance 1 year, strain
2.5 cun plant of the width greater than 22cm change basin into 3 cun of transparent nutritive cubes, and matrix is bark.Maintenance management condition: optimum humidity
It is 60 to 80%, when humidity is lower than 60%, ground sprinkler, spraying, cascade ventilation near potting pocket orchid；Temperature condition daytime 18
DEG C to 28 DEG C, 15 DEG C to 18 DEG C of night；Using obscurity 75% shading net by pocket orchid light control 10000lux extremely
15000lux.Water and fertilizer management condition: bark matrix 8 at it is dry when moisturizing (generally require pour for 5 to 7 days primary).Watering will pour every time
(rainy day does not water) thoroughly.Concentration of fertilizer is 3000 times, and fertilising in vegetative growth phase 2 weeks is primary, pours root zone using liquid fertilizer and combines
Spray leaf surface.Pocket orchid growth period is specific to commonly use the compound fertilizer that N:P:K is 20:20:20 based on nitrogenous fertilizer and balanced fertilizer.
1.5 cun plant of 1 year, plant width greater than 9cm will be conserved and change basin into 2.5 cun of transparent nutritive cubes, after maintenance 1 year, strain
2.5 cun plant of the width greater than 22cm change basin into 3 cun of transparent nutritive cubes, and matrix is sphagna: bark=1:1.Maintenance management item
Part: 18 DEG C to 28 DEG C of daytime, 15 DEG C to 18 DEG C of night.When temperature is higher than 30 DEG C, cooled down by reinforcing ventilation, winter is low
When 15 DEG C, using heat preservation or heating measure；Humidity 50 to 80% when humidity is greater than 80%, reinforces ventilation and air is promoted to flow,
When humidity is lower than 50%, ground sprinkler, spraying, cascade ventilation near potting pocket orchid；Pocket blue streak shines using obscurity 75%
Shading net control in 10000lux to 15000lux.Water and fertilizer management condition: sphagna add bark be matrix when, pour one within 7 to 10 days
It is secondary.Watering will irrigate (rainy day does not water) every time.Concentration of fertilizer is 3000 times, and fertilising in vegetative growth phase 2 weeks is primary, and reproduction is raw
Fertilising in long-term 1 month is primary, and fertilising pours root zone using liquid fertilizer and sprays leaf surface progress.Pocket orchid vegetative growth phase with nitrogenous fertilizer and
Based on balanced fertilizer, specifically used N:P:K is the compound fertilizer of 30:10:10 and 20:20:20.The flower forcing date is before Target Flowering 6
A month, day night temperature was controlled in 25 to 27 DEG C/16 to 18 DEG C progress flower forcing processing, intensity of illumination 10000lux to 15000lux.
Florescence reduces fertilising, and fertilising in every 4 weeks is primary, properly increases P fertilizer, and specifically used N:P:K is the compound fertilizer of 10:30:10, passes through liquid
Fertilizer pours root zone and sprays leaf surface progress.
Under this method operation, hybrid seed aseptic seeding is not sprouted or germination rate is extremely low, and pocket orchid species is extremely immature (white
Color group's dress) or mature (brown particles shape), be difficult to sprout.Matrix is sphagna after tissue-cultured seedling bottle outlet, and survival rate is lower than 40%.
Middle seedlings matrix is bark, and root system development is good, but blade is not stood upright and minus green, and growth cycle is long, and Flowering quality is poor, Hua Jing little
In 10cm, pattern is shallow (referring to Fig. 7 a).
Embodiment 7
The mature seedling in 3 years of meat pie series pocket orchid species described in selection example 1.The plant for selecting healthy mature, is opening
Hua Hou carries out artificial autocopulation's pollination.When pollination, remove the lip of Parent first, chooses lower male parent flower powder with needle, be placed in mother
On this column cap, because pollen load has stickum, therefore, pollen grain can be sticked on column cap.Fruit pod after selection pollination 240 days
Seed (seed is half ripe, is in light yellow graininess) is obtained, seed germination medium is made, is formulated as 1/4MS+1mg/L 6-
BA+0.1mg/L NAA+1g/L peptone+25g/L banana+5.5g/L agar+1g/L active carbon.By the white seed in fruit pod
It is seeded on the culture medium made.After sowing 30 to 40 days, seed starts to sprout, and the dotted tissue of white occurs, and turns green,
There is green bud point, measures germination rate at this time.
After sowing 100 days to 150 days, the sprout for having developed blade is transferred to strong seedling culture base, is formulated as 1/4MS+
4mg/L 6-BA+0.2mg/L NAA+25g/L banana+5.5g/L agar+1g/L active carbon.
Band bottle, which is transferred under natural light cultivate, when will grow to plant height 4 in strong seedling culture base to 6cm carries out experienced seedling for 20 days.
Through white silk seedling after, take out plant, wash root culture medium with 1000 times of dilutions of Baijunling, then transplant in
In 1.7 cun of transparent nutritive cubes, matrix is bark.Maintenance management condition: humid control is 60 to 80%, when humidity is lower than 60%,
Ground sprinkler, spraying, cascade ventilation near potting pocket orchid；18 DEG C to 28 DEG C, 15 DEG C to 18 DEG C of night of temperature condition daytime；Pocket
Blue streak impinges upon 10000lux to 15000lux, carries out shading control in 75% shading net using obscurity.Water and fertilizer management condition:
Bark matrix 8 at it is dry when moisturizing (generally require pour for 5 to 7 days primary).Watering will irrigate (rainy day does not water) every time.Fertilizer is dense
Degree is 3000 times, and fertilising in vegetative growth phase 2 weeks is primary, is combined using liquid fertilizer pouring root zone and sprays leaf surface.Pocket orchid growth period with
Based on nitrogenous fertilizer and balanced fertilizer, specifically used N:P:K is the compound fertilizer of 20:20:20.
1.5 cun plant of 1 year, plant width greater than 9cm will be conserved and change basin into 2.5 cun of transparent nutritive cubes, after maintenance 1 year, strain
2.5 cun plant of the width greater than 22cm change basin into 3 cun of transparent nutritive cubes, and matrix is sphagna: bark=1:1.Maintenance management item
Part: 18 DEG C to 28 DEG C of daytime, 15 DEG C to 18 DEG C of night.When temperature is higher than 30 DEG C, cooled down by reinforcing ventilation, winter is low
When 15 DEG C, using heat preservation or heating measure；Humidity 50 to 80% when humidity is greater than 80%, reinforces ventilation and air is promoted to flow,
When humidity is lower than 50%, ground sprinkler, spraying, cascade ventilation near potting pocket orchid；Pocket blue streak shines using obscurity 75%
Shading net control in 10000lux to 15000lux.Water and fertilizer management condition: sphagna add bark be matrix when, pour one within 7 to 10 days
It is secondary.Watering will irrigate (rainy day does not water) every time.Concentration of fertilizer is 3000 times, and fertilising in vegetative growth phase 2 weeks is primary, and reproduction is raw
Fertilising in long-term 1 month is primary, and fertilising is combined using liquid fertilizer pouring root zone and sprays leaf surface progress.Pocket orchid vegetative growth phase is with nitrogenous fertilizer
And based on balanced fertilizer, specifically used N:P:K is the compound fertilizer of 20:20:20.
The progress flower forcing in 6 months before Target Flowering, day night temperature is controlled in 25 to 27 DEG C/16 to 18 DEG C during flower forcing
Carry out flower forcing processing, intensity of illumination 10000lux to 15000lux.Florescence reduces fertilising, and fertilising in every 4 weeks is primary, properly increases P
Fertilizer, specifically used N:P:K are the compound fertilizer of 10:30:10, pour root zone combination by liquid fertilizer and spray leaf surface progress.
Under this method operation, select to carry out sowing sprouting when seed half ripe (being in light yellow graininess 240 days after pollination)
Rate is high, and emergence is neat.It uses survival rate and growth speed of the basal medium for 1/4MS when high, adds peptone in culture medium
Germination rate can be improved with banana.
Matrix is bark after tissue-cultured seedling bottle outlet, and survival rate may be up to 85% or more, even up to 100%.Middle seedlings matrix
For sphagna: when bark=1:1, root system development is good, and blade stands upright, is blackish green, wide and thick, substantially reduces nutrient growth week
Phase, Flowering quality is good, and for Hua Jing up to 12 to 16cm, pattern is gorgeous and beautiful (referring to Fig. 7 b).
The quantity of blooming for the big flower pattern pocket orchid cultivated by the method for the invention is more, and appreciation effect is good.Filial generation germination rate
Height, bottle seedling high survival rate, big seedling leaf stand upright, are blackish green, wide and thick, large flower and brilliant color, and the florescence is up to two half a months, production cycle
It drops to 3 years.This technology is applied to big flower pattern pocket orchid breeding and production, while shortening the production cycle, guarantees product quantity and matter
Amount provides technology and guarantees for big flower pattern pocket orchid breeding and the production of potted flower.
Claims (19)
1. a kind of method for improving big flower pattern pocket orchid seed germination rate, which is characterized in that described method includes following steps:
(1) artificial pollination is carried out in big flower pattern pocket orchid Post flowering；With
(2) it 180 days to 270 days acquisition seeds and is seeded on seed germination medium after pollination, to be obtained by seed
Sprout；
Wherein, the seed germination medium is 1/4MS+1mg/L 6-BA+0.1mg/L NAA+1g/L peptone+25g/L fragrant
Any of several broadleaf plants+5.5g/L agar+1g/L active carbon.
2. the method according to claim 1, wherein the big flower pattern pocket orchid is the pocket orchid product that flower is greater than 12cm
Kind.
3. according to the method described in claim 2, it is characterized in that, the big flower pattern pocket orchid is the big flower pattern pocket orchid species of F1 generation.
4. according to the method described in claim 2, it is characterized in that, the big flower pattern pocket orchid is meat pie series pocket orchid species.
5. method according to claim 1 to 4, which is characterized in that the seed is that 210 days extremely after pollination
240 days seeds obtained.
6. method according to claim 1 to 4, which is characterized in that the seed is to obtain for 240 days after pollination
The seed taken.
7. method according to claim 1 to 4, which is characterized in that the artificial pollination be pollination self and/
Or hybridization pollination.
8. method according to claim 1 to 4, which is characterized in that the artificial pollination is hybridization pollination.
9. a kind of cultural method of big flower pattern pocket orchid, which is characterized in that the method uses any one of claims 1 to 8 institute
The method stated improves seed germination rate.
10. according to the method described in claim 9, it is characterized in that, blade will have been developed after sowing 100 days to 150 days
Sprout be transferred in strong seedling culture base and cultivate to obtain tissue-cultured seedling, the formula of the strong seedling culture base is 1/4MS+4mg/L 6-BA
+ 0.2mg/L NAA+25g/L banana+5.5g/L agar+1g/L active carbon.
11. according to the method described in claim 10, it is characterized by:
When the plant height of the tissue-cultured seedling is 4cm to 6cm, after tissue-cultured seedling is transferred to natural light lower refining seedling 20 days, by tissue-cultured seedling
Be transplanted in the cultivating container equipped with bark matrix, and conserved with following condition: relative humidities be 60% to
80%；Temperature condition be 18 DEG C to 28 DEG C of daytime, 15 DEG C to 18 DEG C of night；Illumination condition is 10000lux to 15000lux；Water
Fertile management condition is to sprinkle profoundly water within every 5 to 7 days the compound fertilizer primary, application N:P:K is 30:10:10 and/or 20:20:20, dilution
Multiple is 3000 times, and frequency of administration is that 2 to 4 weeks are primary, and method of application is root-zone irrigation and/or foliage-spray；
It carries out once changing basin when strain width is equal to or more than 9cm, and carries out when strain width is equal to or more than 22cm secondary
Basin is changed, the matrix that basin uses is changed to be the sphagna for being 1:1 by mass ratio and bark forms, and conserved with following condition:
Relative humidities are 60% to 80%；Temperature condition be 18 DEG C to 28 DEG C of daytime, 15 DEG C to 18 DEG C of night；Illumination condition is
10000lux to 15000lux；Water and fertilizer management condition is to sprinkle profoundly water within every 7 to 10 days once, and application N:P:K is answering for 20:20:20
Hefei, extension rate are 3000 times, and frequency of administration is that once every 2 weeks, method of application is root-zone irrigation and/or foliage-spray；
It is secondary change basin after and the progress flower forcing in 6 months before Target Flowering, conserved during flower forcing with following condition:
Relative humidities are not less than 50%；Temperature condition be 25 DEG C to 27 DEG C of daytime, 16 DEG C to 18 DEG C of night；Illumination condition is
10000lux to 15000lux；Water and fertilizer management condition is to sprinkle profoundly water within every 7 to 10 days once, and application N:P:K is answering for 10:30:10
Hefei, extension rate are 3000 times, and frequency of administration is 4 weeks primary, and method of application is root-zone irrigation and/or foliage-spray.
12. according to the method for claim 11, which is characterized in that the relative humidity item that the maintenance of tissue-cultured seedling container culturing uses
Part is 80%.
13. according to the method for claim 11, which is characterized in that change the relative humidities that basin maintenance uses as 80%.
14. according to the method for claim 11, which is characterized in that conserved during flower forcing the relative humidities that use for
80%.
15. method described in any one of 1 to 14 according to claim 1, it is characterised in that:
The bark matrix used when transplanting after hardening is pine bark；And/or
Bark contained in the matrix used when changing basin is pine bark.
16. according to the method for claim 15, which is characterized in that the bark matrix used when transplanting after hardening is pine
Squama.
17. according to the method for claim 15, which is characterized in that used when it is characterized in that, being transplanted after hardening described
The size of the bark of bark matrix is 3mm to 7mm.
18. according to the method for claim 15, which is characterized in that bark contained in the matrix used when changing basin is pine
Squama.
19. according to the method for claim 15, which is characterized in that the ruler of bark contained in the matrix used when changing basin
Very little is 10mm to 20mm.
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| CN201811313552.2A CN109287486B (en) | 2016-09-09 | 2016-09-09 | Paphiopedilum seed germination rate improving method and paphiopedilum cultivation method |
| CN201610811368.5A CN106386488B (en) | 2016-09-09 | 2016-09-09 | It is a kind of to improve big flower pattern pocket orchid seed germination rate and its cultural method |
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| CN107326044A (en) * | 2017-07-28 | 2017-11-07 | 黔西南州绿缘动植物科技开发有限公司 | A kind of transgenosis spends the blue implantation methods of pocket in vain |
| CN110089428B (en) * | 2019-04-10 | 2022-07-08 | 福建省农业科学院作物研究所 | Paphiopedilum hybrid seed sterile seeding and seedling raising method |
| CN110149950B (en) * | 2019-06-06 | 2021-12-03 | 广西壮族自治区中国科学院广西植物研究所 | Method for improving development quality of paphiopedilum albolanum seeds and test solution thereof |
| CN110881478B (en) * | 2019-11-07 | 2021-04-13 | 中国林业科学研究院林业研究所 | Method for promoting germination of seeds of denatolum and paphiopedilum by using ceriferous fungi |
| CN115039655A (en) * | 2022-07-18 | 2022-09-13 | 中国科学院昆明植物研究所 | Standardized potted flower cultivation method for foliage begonia |
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| CN101569285B (en) * | 2009-06-02 | 2011-10-26 | 中国科学院华南植物园 | Method for cross breeding and seedling propagation of paphiopedilum |
| CN101558744B (en) * | 2009-06-04 | 2011-11-23 | 贵州省亚热带作物研究所 | Method for breeding test-tube plantlets of Paphiopedilum henryanum Braem seeds |
| CN102283114B (en) * | 2011-06-23 | 2016-01-13 | 中国科学院华南植物园 | The broad-spectrum culture that orchid aseptic seeding becomes seedling breeding method with test tube and adopts |
| CN103416294A (en) * | 2013-08-06 | 2013-12-04 | 广西壮族自治区中国科学院广西植物研究所 | Concolor paphiopedilum crossbreeding method and seedling breeding method thereof |
| CN105052750A (en) * | 2015-09-08 | 2015-11-18 | 广西壮族自治区农业科学院花卉研究所 | Crossbreeding and seedling rapid propagation method of paphiopedilum pacific shamrock |
| CN105052751A (en) * | 2015-09-08 | 2015-11-18 | 广西壮族自治区农业科学院花卉研究所 | Method for determining optimal picking period of capsules of paphiopedilum concolor |
| CN105284366B (en) * | 2015-10-30 | 2018-01-26 | 华南农业大学 | A kind of method for shortening pocket orchid fruit development period and improving seed germination rate |
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| CN106386488A (en) | 2017-02-15 |
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