CN113016474A - Zanthoxylum nitidum seedling raising method - Google Patents
Zanthoxylum nitidum seedling raising method Download PDFInfo
- Publication number
- CN113016474A CN113016474A CN202110234858.4A CN202110234858A CN113016474A CN 113016474 A CN113016474 A CN 113016474A CN 202110234858 A CN202110234858 A CN 202110234858A CN 113016474 A CN113016474 A CN 113016474A
- Authority
- CN
- China
- Prior art keywords
- seeds
- seedling raising
- seedling
- seedlings
- zanthoxylum nitidum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000003248 Zanthoxylum nitidum Species 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000002689 soil Substances 0.000 claims abstract description 50
- 238000009331 sowing Methods 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 24
- 239000004576 sand Substances 0.000 claims abstract description 21
- 238000005507 spraying Methods 0.000 claims abstract description 20
- 238000002791 soaking Methods 0.000 claims abstract description 17
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 14
- 239000003337 fertilizer Substances 0.000 claims abstract description 14
- 238000003475 lamination Methods 0.000 claims abstract description 8
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 7
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 7
- 238000003892 spreading Methods 0.000 claims abstract description 7
- 230000007480 spreading Effects 0.000 claims abstract description 7
- 238000003306 harvesting Methods 0.000 claims abstract description 5
- 239000000758 substrate Substances 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 23
- 235000013399 edible fruits Nutrition 0.000 claims description 20
- 238000002156 mixing Methods 0.000 claims description 16
- 239000011159 matrix material Substances 0.000 claims description 12
- 239000003415 peat Substances 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 10
- 230000007613 environmental effect Effects 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 6
- 239000004202 carbamide Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000003595 mist Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 239000010455 vermiculite Substances 0.000 claims description 6
- 235000019354 vermiculite Nutrition 0.000 claims description 6
- 229910052902 vermiculite Inorganic materials 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000010451 perlite Substances 0.000 claims description 5
- 235000019362 perlite Nutrition 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 230000014284 seed dormancy process Effects 0.000 claims description 4
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 4
- 238000013517 stratification Methods 0.000 claims description 4
- 229920001542 oligosaccharide Polymers 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 244000126010 Pithecellobium dulce Species 0.000 claims 1
- 235000002194 Pithecellobium dulce Nutrition 0.000 claims 1
- 235000007891 Pithecellobium lobatum Nutrition 0.000 claims 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000001737 promoting effect Effects 0.000 abstract description 6
- 238000011161 development Methods 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000005059 dormancy Effects 0.000 abstract description 2
- 238000010030 laminating Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 15
- 239000010410 layer Substances 0.000 description 13
- 230000035784 germination Effects 0.000 description 10
- 239000006260 foam Substances 0.000 description 8
- 235000016709 nutrition Nutrition 0.000 description 7
- 230000035764 nutrition Effects 0.000 description 7
- 229930191978 Gibberellin Natural products 0.000 description 5
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 5
- 239000003448 gibberellin Substances 0.000 description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 239000004519 grease Substances 0.000 description 4
- 239000011259 mixed solution Substances 0.000 description 4
- 239000012286 potassium permanganate Substances 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 230000007226 seed germination Effects 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 241001048891 Jatropha curcas Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 241001093501 Rutaceae Species 0.000 description 1
- 208000004078 Snake Bites Diseases 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 241000949456 Zanthoxylum Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229910001562 pearlite Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 208000004371 toothache Diseases 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/28—Raised beds; Planting beds; Edging elements for beds, lawn or the like, e.g. tiles
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a Zanthoxylum nitidum seedling raising method, which comprises the following steps: s1: selecting Zanthoxylum nitidum plants as mother trees, and harvesting mature, healthy and plump seeds; s2: soaking the seeds in a sodium bicarbonate solution or a sodium hydroxide solution, laminating at a low temperature, and breaking the dormancy of the seeds; s3: the seeds after the lamination treatment and river sand are sowed with the sowing density of 20-50 g/m2Uniformly spreading the seeds on a seedbed, and not covering surface soil after sowing; or dibbling the laminated seeds in a seedling raising container filled with a seedling raising substrate, wherein the dibbling depth is 0-1 cm; s4: seedling management, keeping seedbed or seedling substrate wet, and standing for seed germinationAnd spraying fertilizer water once after two main leaves of the seedlings grow, and taking the seedlings out of the nursery for field planting when the height of the seedlings is 10-15 cm. The method can effectively shorten the period of raising seedlings of the shinyleaf pricklyash root and improve the survival rate and quality of the seedlings, and has important significance for promoting production of shinyleaf pricklyash root medicinal materials and industrial development of the shinyleaf pricklyash root medicinal materials.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine breeding and agricultural planting. More specifically, the invention relates to a method for raising seedlings of Zanthoxylum nitidum seedlings.
Background
Radix Zanthoxyli is a medicinal material of Guangxi province, is derived from dried root of Zanthoxylum nitidum (Roxb.) DC of Zanthoxylum of Rutaceae, has effects of promoting blood circulation for removing blood stasis, activating qi-flowing for relieving pain, dispelling pathogenic wind for dredging collaterals, removing toxic substance and detumescence, and can be used for treating traumatic injury, stomach ache, toothache, rheumatalgia, venomous snake bite, and external treatment of burn and scald. The Guangxi is a big province of Zanthoxylum nitidum resources and production and marketing, the Zanthoxylum nitidum plants are distributed in different climatic zones of the Guangxi, and the content of the nitidissima medicine, namely the nitidissima chloride, in the Guangxi is the highest. However, since a long time ago, the Zanthoxylum nitidum medicinal materials are always from wild resources, and unreasonable forest destruction is wasted, so that the Zanthoxylum nitidum resources are reduced year by year, and the rapid propagation and artificial cultivation research of Zanthoxylum nitidum also becomes more and more important. The Zanthoxylum nitidum is mainly used for seed reproduction, the seeds which are picked and sowed are the most orderly to sprout at the earliest, the seeds are sowed in 10 months and sprout and come out in 5 months next year, and the sprouting time is as long as 180 days. The germination limiting conditions of the Zanthoxylum nitidum seeds comprise seed coat water absorption barrier, immature embryos, germination inhibitors and the like, seed dormancy can not be broken through concentrated sulfuric acid, dilute hydrochloric acid, dilute sodium hydroxide, warm soup seed soaking and the like, and seed dormancy can be broken through low-temperature wet sand stratification treatment, gibberellin treatment and the like to a certain extent. Due to the fact that the quality of the zanthoxylum nitidum seeds is different, the germination period is long, and the germination rate is low, the work popularization of the zanthoxylum nitidum seeds is difficult to carry out, the fine variety breeding, the large-scale cultivation production and the like, and the development of the zanthoxylum nitidum resources in the traditional Chinese medicine industry and the daily chemical industry is severely restricted.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
The invention also aims to provide a radix zanthoxyli seedling raising method, which can effectively shorten the radix zanthoxyli seedling raising period, improve the seedling survival rate and the seedling quality and has important significance for promoting the production of radix zanthoxyli medicinal materials and the industrial development thereof.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for raising seedlings of Zanthoxylum nitidum seedlings, comprising the steps of:
s1: selecting Zanthoxylum nitidum plants as mother trees, and harvesting mature, healthy and plump seeds;
s2: soaking the seeds in sodium bicarbonate solution or sodium hydroxide solution, taking out, washing in clear water, low-temperature layering, and breaking seed dormancy;
s3: the seeds after the lamination treatment and river sand are sowed with the sowing density of 20-50 g/m2Uniformly spreading on a seedbed, not covering surface soil after sowing, sterilizing the seedbed before spreading, airing to dry, and watering thoroughly;
or dibbling the laminated seeds in a seedling raising container filled with a seedling raising matrix, wherein the dibbling depth is 0-1 cm, and the seedling raising matrix is spread for 1-2 days after being watered thoroughly before dibbling;
s4: and (3) seedling management, namely keeping the environmental temperature at 20-30 ℃, spraying water mist to keep a seedbed or a seedling medium moist, spraying fertilizer water for one time after the seedlings grow into two true leaves after the seeds germinate, and taking out of the nursery for field planting when the height of the seedlings is 10-15 cm.
Preferably, in S1, gather more than 2 ~ 3 years, illumination is sufficient, healthy two-side needle plant is mother tree, 9 ~ 10 months, when the shell of whole-length fruit turn into purple red by green to harvest ripe fruit, the whole-length fruit of stem that will pluck is arranged in and is ventilated, shady and cool, dry department carries out the shade 5 ~ 7d, treat the shell fracture after, it makes the pod burst open to beat, detach the fruit stalk, the shell, empty flat seed, select black beaded full healthy seed.
Preferably, in S2, the specific manner of seed soaking is as follows: and (3) soaking the seeds in a baking soda solution with the mass fraction of 1-5% for 5-8 h, taking out the seeds, and washing the seeds in clear water.
Preferably, in S2, the specific manner of seed soaking is as follows: soaking in 1-2% sodium hydroxide solution for 5-10 min, taking out, and washing in clear water.
Preferably, in S2, the specific form of the low-temperature lamination is: uniformly mixing the seeds and the wet sand according to the volume ratio of 1:3, and keeping the environmental temperature at 0-5 ℃ for low-temperature stratification for 30-50 d.
Preferably, in S3, the seedbed comprises a river sand layer with the depth of 5-10 cm, a mixed soil layer with the depth of 5-10 cm and a light matrix layer with the depth of 3-5 cm, which are sequentially arranged from bottom to top, wherein the mixed soil layer is formed by uniformly mixing river sand and garden soil according to the volume ratio of 1:1, and the light matrix layer is formed by uniformly mixing vermiculite, peat soil and perlite according to the volume ratio of 1:1: 1.
Preferably, in S3, the seedling substrate is prepared by mixing river sand and garden soil according to the volume ratio of 1:1, or is prepared by mixing vermiculite, peat soil and perlite according to the volume ratio of 1:1: 1.
Preferably, in S4, the fertilizer water is a mixed water solution of 0.1-0.5% by mass of urea and 0.1-0.5% by mass of monopotassium phosphate.
Preferably, sodium hypochlorite solution with the mass fraction of 2% is sprayed on the surfaces of garden soil and peat soil, the garden soil and peat soil are dried in the sun, and mixed aqueous solution of amino-oligosaccharin with the mass concentration of 0.5g/L and glucose with the mass concentration of 1g/L is sprayed until the surfaces are wet and do not cake.
The invention at least comprises the following beneficial effects:
firstly, soaking seeds by using a sodium bicarbonate solution or a sodium hydroxide solution to break dormancy of the zanthoxylum nitidum seeds, so that the emergence rate and the emergence uniformity of the zanthoxylum nitidum seeds are improved; the survival rate of the seedlings is increased by matching a shallow sowing mode of broadcasting or dibbling with a proper seedbed or seedling raising substrate; the growth of the nursery stock is effectively promoted by frequently applying the fertilizer water, and the quality of the nursery stock is improved.
Compared with the conventional seedling raising method, the method can effectively shorten the seedling raising period of the shinyleaf pricklyash root and improve the survival rate and quality of the seedlings, and has important significance for promoting production of shinyleaf pricklyash root medicinal materials and industrial development of the shinyleaf pricklyash root medicinal materials.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is a schematic representation of a Zanthoxylum nitidum fruit of the present invention;
FIG. 2 is a schematic view of a Zanthoxylum nitidum seed of the present invention;
FIG. 3 is a schematic view of a Zanthoxylum nitidum seedling of the present invention;
FIG. 4 is a schematic representation of a Liangmianzhen seedling Nanning nursery of the present invention;
fig. 5 is a schematic view of the jatropha curcas nursery of the present invention.
Detailed Description
The present invention is further described in detail below with reference to examples to enable those skilled in the art to practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.
Yulin field test:
in 2018, seedlings of Zanthoxylum nitidum are cultivated in a Dachow colored rural area (Dongding longitude 110 degrees, 17 '52.20' and northern latitude 22 degrees, 39 '26.06').
< example 1>
Step 1: collecting Zanthoxylum nitidum in a five-color garden in 2018, 10 and 3 days, spreading and drying in the shade in the room as shown in figure 1, knocking fruit pods by a wood stick for threshing after 10 and 8 days, manually picking out impurities such as fruit stalks, fruit shells and the like, and screening empty and shriveled grains to obtain clean seeds, wherein the weight of the seeds in hundred grains is 3.22g as shown in figure 2.
Step 2: soaking 0.8kg of clean seeds in 10L of 2% sodium bicarbonate solution for 8h, taking out, washing in clear water for 3 times, uniformly mixing the seeds and wet sand according to the volume ratio of 1:3, filling into a foam box, sealing, placing the foam box in a refrigerator at 5 ℃ for low-temperature lamination for 30d, and taking out and sowing in 8 days of 11 months.
And step 3: in the seedling raising shed in 26 days in 10 months and at 30m2The method comprises the steps of building 4 seedling culture ponds on a seedling culture field, wherein each pond is 1m wide, 10m long and 30cm deep, paving a layer of river sand with the thickness of 10cm at the bottom of each pond, paving a layer of mixed soil with the thickness of 10cm and the volume ratio of garden soil being 1:1 in the middle of each pond, paving a layer of mixed light matrix with the thickness of 5cm on the surface layer and the volume ratio of vermiculite, peat soil and perlite being 1:1:1, spraying 0.1% potassium permanganate solution for disinfection, airing for 11 months and 5 days, and then watering the seedbeds thoroughly. Sowing in 8 days in 11 months, uniformly scattering the stacked seeds on a seedbed, covering no surface soil after sowing, then building a plastic film arched shed with the height of 30cm on a seedling pool, keeping the environmental temperature at 20-30 ℃, and spraying water mist for 10min at 10 am every day in the shed to keep the seedbed moist. After the seeds germinate, after the seedlings grow out of two true leaves, spraying a leaf fertilizer once, wherein the leaf fertilizer is a mixed solution of 0.5% of urea and 0.1% of monopotassium phosphate.
45d after sowing, 23 days 12 months, the field emergence rate is checked to be 68%, and the seedlings are shown in figure 3. After 3 months and 8 days 2 months after sowing, the detected seedling rate is 59%, the total number of seedlings is about 1.5 ten thousand, the average plant height is 7.5cm, and the two-side needle seedling nursery is shown in figure 5. The technology shortens the production cycle of the Zanthoxylum nitidum seedlings from 1 year to 4 months in the conventional method, namely, from 10 and 3 days in 2018 to 2 and 8 days in 2019, and improves the germination rate of the seeds from 0-10% in a natural state to 68%.
< example 2>
Step 1-2 the same as example 1 except that step 3:
and step 3: in the seedling raising shed in 26 days in 10 months and at 30m2Building 4 seedling raising ponds on the seedling raising field, wherein each pond is 1m wide, 10m long and 30cm deep, pretreating garden soil and peat soil, spraying a sodium hypochlorite solution with the mass fraction of 2%, drying in the sun, spraying a mixed aqueous solution of amino-oligosaccharide with the mass concentration of 0.5g/L and glucose with the mass concentration of 1g/L until the surfaces are wet and do not cake, paving a layer of river sand with the thickness of 10cm at the bottom of the pond, paving a layer of mixed soil with the thickness of 10cm and the volume ratio of the river sand to the garden soil of 1:1 at the middle of the pond after the pretreatment is finished, and paving a layer of vermiculite, peat soil and pearl soil with the thickness of 5cm on the surface layerSpraying 0.1% potassium permanganate solution on the mixed light matrix with the volume ratio of the pearlite being 1:1:1 for disinfection, airing for 11 months and 5 days, and then thoroughly watering the seedbed. Sowing in 8 days in 11 months, uniformly scattering the stacked seeds on a seedbed, covering no surface soil after sowing, then building a plastic film arched shed with the height of 30cm on a seedling pool, keeping the environmental temperature at 20-30 ℃, and spraying water mist for 10min at 10 am every day in the shed to keep the seedbed moist. After the seeds germinate, after the seedlings grow out of two true leaves, spraying a leaf fertilizer once, wherein the leaf fertilizer is a mixed solution of 0.5% of urea and 0.1% of monopotassium phosphate.
And 4, checking that the field emergence rate is 72% 45d after sowing and 23 days in 12 months. 3 months after sowing, 2 months and 8 days, the checked seedling rate is 63 percent, the total number of seedlings is about 1.6 ten thousand, and the average plant height is 7.8 cm.
The method is characterized in that garden soil and peat soil are pretreated, so that the method has a direct inhibiting effect on various pathogenic bacteria, simultaneously activates plant cells, is decomposed and utilized by microorganisms and serves as nutrients for plant growth, and a plastic arched shed can keep warm and moisturize, maintain stable seedling culture environment and is beneficial to seedling growth.
< comparative example 1>
Steps 1 and 3 are the same as in example 1 except that step 2:
soaking 0.3kg of clean seeds in gibberellin solution with the concentration of 200mg/L for 18h, fishing out, putting in clear water, washing for 3 times, uniformly mixing the seeds and wet sand according to the volume ratio of 1:3, putting in a foam box, sealing, placing the foam box in a refrigerator with the temperature of 5 ℃ for low-temperature lamination for 30d, and taking out and sowing in 11 months and 8 days. And 3, broadcasting and raising seedlings in 1 seedling raising pond.
And 4, checking that the field emergence rate is 9% 45d after sowing and 23 days in 12 months. 3 months after sowing, 2 months and 8 days, the checked seedling rate is 11 percent, the total seedling number is about 0.1 ten thousand plants, and the average plant height is 7.4 cm.
The reason is that the seed surface of the Zanthoxylum nitidum has a layer of grease, the seed shell is hard and compact in structure, gibberellin cannot remove the grease, the seed coat cannot be softened, and the effect of promoting seed germination cannot be achieved, so that the seed germination speed is still slow, and the germination time is still long.
< comparative example 2>
Step 1-2 the same as example 1 except that step 3:
and 3, sowing and raising seedlings in 1 seedling raising pond, uniformly scattering the stacked seeds at the depth of 2cm of a seedbed, and covering surface soil after sowing.
And 4, checking that the field emergence rate is 26% 45d after sowing and 23 days in 12 months. 3 months after sowing, 2 months and 8 days, the checked seedling rate is 30%, the total number of seedlings is about 0.75 ten thousand, and the average plant height is 7.0 cm.
This is because the sowing depth is large, the air permeability in the matrix is poor, and the germination of the Zanthoxylum nitidum seeds is not facilitated.
Field test of Nanning:
the seedlings of the Zanthoxylum nitidum are cultivated in Guangxi medicinal plant gardens (east longitude 108 degrees 22 '11.21', north latitude 22 degrees 51 '30.42') of Nanning city in 2018.
< example 3>
Step 1: and harvesting Zanthoxylum nitidum fruits in the plant garden in 2018, 9 and 22 days, spreading and drying in the shade in the room as shown in figure 1, knocking a fruit pod by a wood stick to thresh the seeds after 9 and 25 days, manually picking out impurities such as fruit stalks, fruit shells and the like, and screening empty and shriveled grains to obtain the seeds with the total weight of 12.4kg and the hundred-grain weight of 3.51g, namely the total grain number of about 35.3 ten thousand grains as shown in figure 2.
Step 2: soaking 3kg of clean seeds in 50L of 1% sodium hydroxide solution for 10min, taking out, washing with clear water for 5 times, mixing the seeds and wet sand uniformly according to the volume ratio of 1:3, placing in a foam box, sealing, placing the foam box in a refrigerator at 5 ℃ for low-temperature lamination for 40d, and taking out and sowing for 5 days in 11 months.
And step 3: and (3) leveling the ground in a seedling raising shed within 10 months and 26 days, mixing river sand and garden soil according to the volume ratio of 1:1, spraying 0.1% potassium permanganate solution for disinfection, and airing until the soil is dry. And (3) filling the mixed soil into a 10cm nutrition pot in 11 months and 1 day, putting the mixed soil to the ground of a seedbed in order, and then installing an atomizing nozzle on the seedbed to thoroughly water the nutrition pot. Sowing in 5 days after 11 months, dibbling the laminated seeds on the surface soil of each nutrition pot, then building a shading net with the height of 50cm on the seedlings, keeping the environmental temperature at 20-30 ℃, and spraying water mist for 10min at 10 am and 6 pm in a shed every day to keep the seedbed moist. After the seeds germinate, after the seedlings grow out of two true leaves, spraying a leaf fertilizer once, wherein the leaf fertilizer is a mixed solution of 0.5% of urea and 0.1% of monopotassium phosphate.
45d after sowing, 20 days 12 months, the field emergence rate is checked to be 75%, and the seedlings are shown in figure 3. After 3 months and 5 days 2 months after sowing, the detected seedling rate is 65%, the total number of seedlings is about 5.5 ten thousand, the average plant height is 7.9cm, and the two-side needle seedling nursery is shown in figure 4. The technology shortens the production cycle of the Zanthoxylum nitidum seedlings from 1 year of the conventional method to 4 and a half months, namely, from 22 days in 9 months in 2018 to 5 days in 2 months in 2019, and improves the germination rate of the seeds from 0-10% in a natural state to 75%.
< example 4>
Step 1-2 the same as example 1 except that step 3:
and step 3: leveling the ground in a seedling raising shed in 26 days after 10 months, simultaneously pretreating garden soil, spraying a sodium hypochlorite solution with the mass fraction of 2%, drying in the sun, spraying a mixed aqueous solution of amino-oligosaccharin with the mass concentration of 0.5g/L and glucose with the mass concentration of 1g/L until the surface is wet and does not cake, after the pretreatment is finished, mixing river sand and garden soil according to the volume ratio of 1:1, spraying a potassium permanganate solution with the mass concentration of 0.1%, disinfecting, and drying in the sun until the garden soil is dry. And (3) filling the mixed soil into a 10cm nutrition pot in 11 months and 1 day, putting the mixed soil to the ground of a seedbed in order, and then installing an atomizing nozzle on the seedbed to thoroughly water the nutrition pot. Sowing in 5 days after 11 months, dibbling the laminated seeds on the surface soil of each nutrition pot, then building a shading net with the height of 50cm on the seedlings, keeping the environmental temperature at 20-30 ℃, and spraying water mist for 10min at 10 am and 6 pm in a shed every day to keep the seedbed moist. After the seeds germinate, after the seedlings grow out of two true leaves, spraying a leaf fertilizer once, wherein the leaf fertilizer is a mixed solution of 0.5% of urea and 0.1% of monopotassium phosphate.
45d after sowing, 20 days in 12 months, and the rate of emergence in the field is checked to be 77%. After 3 months and 5 days after 2 months, the checked seedling rate is 68 percent, the total number of seedlings is about 5.8 ten thousand, and the average plant height is 8.2 cm.
The method is characterized in that garden soil is pretreated, so that the garden soil has a direct inhibition effect on various pathogenic bacteria, simultaneously activates plant cells, is decomposed and utilized by microorganisms and serves as a nutrient for plant growth, and the plastic arched shed can keep warm and moisturize, maintain stable seedling culture environment and facilitate seedling growth.
< comparative example 3>
Steps 1 and 3 are the same as in example 1 except that step 2:
soaking 3kg of clean seeds in a gibberellin solution with the concentration of 200mg/L for 18h, fishing out, washing in clear water for 5 times, uniformly mixing the seeds and wet sand according to the volume ratio of 1:3, filling into a foam box, sealing, placing the foam box in a refrigerator with the temperature of 5 ℃ for low-temperature lamination for 40d, and taking out and sowing in 5 days of 11 months.
And 4, checking that the field emergence rate is 5% 45d after sowing and 20 days 12 months after sowing. 3 months and 5 days after sowing, the detected seedling rate is 9 percent, the total seedling number is about 0.8 ten thousand plants, and the average plant height is 7.5 cm.
The reason is that the seed surface of the Zanthoxylum nitidum has a layer of grease, the seed shell is hard and compact in structure, gibberellin cannot remove the grease, the seed coat cannot be softened, and the effect of promoting seed germination cannot be achieved, so that the seed germination speed is still slow, and the germination time is still long.
< comparative example 4>
Step 1-2 the same as example 1 except that step 3:
and dibbling 3kg of the stacked seeds at the depth of 2cm of soil in each nutrition pot, and backfilling the covering soil to be level.
And 4, checking that the field emergence rate is 28% 45d after sowing and 20 days 12 months after sowing. After 3 months and 5 days after sowing, the checked seedling rate is 25%, the total number of seedlings is about 2.1 ten thousand, and the average plant height is 7.1 cm.
This is because the sowing depth is large, the air permeability in the matrix is poor, and the germination of the Zanthoxylum nitidum seeds is not facilitated.
The number of apparatuses and the scale of the process described herein are intended to simplify the description of the present invention. Applications, modifications and variations of the present invention will be apparent to those skilled in the art.
While embodiments of the invention have been disclosed above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.
Claims (9)
1. The Zanthoxylum nitidum seedling raising method is characterized by comprising the following steps:
s1: selecting Zanthoxylum nitidum plants as mother trees, and harvesting mature, healthy and plump seeds;
s2: soaking the seeds in sodium bicarbonate solution or sodium hydroxide solution, taking out, washing in clear water, low-temperature layering, and breaking seed dormancy;
s3: the seeds after the lamination treatment and river sand are sowed with the sowing density of 20-50 g/m2Uniformly spreading on a seedbed, not covering surface soil after sowing, sterilizing the seedbed before spreading, airing to dry, and watering thoroughly;
or dibbling the laminated seeds in a seedling raising container filled with a seedling raising matrix, wherein the dibbling depth is 0-1 cm, and the seedling raising matrix is spread for 1-2 days after being watered thoroughly before dibbling;
s4: and (3) seedling management, namely keeping the environmental temperature at 20-30 ℃, spraying water mist to keep a seedbed or a seedling medium moist, spraying fertilizer water for one time after the seedlings grow into two true leaves after the seeds germinate, and taking out of the nursery for field planting when the height of the seedlings is 10-15 cm.
2. The Zanthoxylum nitidum seedling raising method according to claim 1, wherein in S1, Zanthoxylum nitidum plants which are grown for 2-3 years or more, sufficiently illuminated and healthy are harvested as mother trees, and after 9-10 months, mature fruits are harvested when the fruit shells of the Zanthoxylum nitidum turn from green to purple, and the harvested whole-length fruits are placed in a ventilated, shady and dry place for shade drying for 5-7 d, and after the fruit shells crack, the fruit shells are knocked to burst the fruit pods, so that the fruit stems, the fruit shells and the empty and flat seeds are removed, and the full and healthy seeds in the shape of black beads are screened out.
3. The radix zanthoxyli seedling raising method according to claim 1, wherein in S2, the seed soaking is specifically performed by: and (3) soaking the seeds in a baking soda solution with the mass fraction of 1-5% for 5-8 h, taking out the seeds, and washing the seeds in clear water.
4. The radix zanthoxyli seedling raising method according to claim 1, wherein in S2, the seed soaking is specifically performed by: soaking in 1-2% sodium hydroxide solution for 5-10 min, taking out, and washing in clear water.
5. The Zanthoxylum nitidum seedling raising method according to claim 1, wherein in S2, the specific manner of low-temperature stratification is: uniformly mixing the seeds and the wet sand according to the volume ratio of 1:3, and keeping the environmental temperature at 0-5 ℃ for low-temperature stratification for 30-50 d.
6. The radix zanthoxyli seedling raising method of claim 1, wherein in S3, the seedbed comprises a river sand layer with a depth of 5-10 cm, a mixed soil layer with a depth of 5-10 cm and a light matrix layer with a depth of 3-5 cm which are sequentially arranged from bottom to top, the mixed soil layer is formed by uniformly mixing river sand and garden soil according to a volume ratio of 1:1, and the light matrix layer is formed by uniformly mixing vermiculite, peat soil and perlite according to a volume ratio of 1:1: 1.
7. The Zanthoxylum nitidum seedling raising method of claim 1, wherein in S3, the seedling raising substrate is prepared by mixing river sand and garden soil according to a volume ratio of 1:1, or is prepared by mixing vermiculite, peat soil and perlite according to a volume ratio of 1:1: 1.
8. The Zanthoxylum nitidum seedling raising method of claim 1, wherein in S4, the fertilizer water is a mixed aqueous solution of urea 0.1-0.5% by mass and potassium dihydrogen phosphate 0.1-0.5% by mass.
9. The Zanthoxylum nitidum seedling raising method according to claim 6 or 7, wherein sodium hypochlorite solution with a mass fraction of 2% is sprayed on the surface of garden soil or peat soil, and dried in the sun, and mixed aqueous solution of amino-oligosaccharide with a mass concentration of 0.5g/L and glucose with a mass concentration of 1g/L is sprayed until the surface is wet and does not cake.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110234858.4A CN113016474B (en) | 2021-03-03 | 2021-03-03 | Seedling raising method for radix zanthoxyli seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110234858.4A CN113016474B (en) | 2021-03-03 | 2021-03-03 | Seedling raising method for radix zanthoxyli seedlings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113016474A true CN113016474A (en) | 2021-06-25 |
| CN113016474B CN113016474B (en) | 2023-09-08 |
Family
ID=76465765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110234858.4A Active CN113016474B (en) | 2021-03-03 | 2021-03-03 | Seedling raising method for radix zanthoxyli seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113016474B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986437A (en) * | 2012-12-14 | 2013-03-27 | 茂名市天一农业科技发展有限公司 | Method for cultivating radix zanthoxyli artificially |
| CN105940893A (en) * | 2016-04-25 | 2016-09-21 | 广西壮族自治区药用植物园 | Quisqualis indica seedling rapid propagation method |
| CN112262712A (en) * | 2020-10-23 | 2021-01-26 | 广西南药康园投资有限责任公司 | High-yield cultivation method for radix zanthoxyli |
-
2021
- 2021-03-03 CN CN202110234858.4A patent/CN113016474B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986437A (en) * | 2012-12-14 | 2013-03-27 | 茂名市天一农业科技发展有限公司 | Method for cultivating radix zanthoxyli artificially |
| CN105940893A (en) * | 2016-04-25 | 2016-09-21 | 广西壮族自治区药用植物园 | Quisqualis indica seedling rapid propagation method |
| CN112262712A (en) * | 2020-10-23 | 2021-01-26 | 广西南药康园投资有限责任公司 | High-yield cultivation method for radix zanthoxyli |
Non-Patent Citations (5)
| Title |
|---|
| 孙世荣;蒋水元;胡永志;李虹;林钻煌;李锋;: "两面针繁殖技术研究" * |
| 时群等: "两面针容器育苗技术" * |
| 赖茂祥;林钻煌;卢栋;覃青云;李永华;黄云峰;胡琦敏;: "两面针规范化生产标准操作规程(SOP)" * |
| 黄宝优;黄雪彦;董青松;韦莹;韦坤华;缪剑华;: "两面针生态种植技术规程" * |
| 黄海连;林伟国;姜成厚;黄演福;余生;王金桥;: "苦玄参种苗繁育技术操作规程(SOP)" * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113016474B (en) | 2023-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105638025B (en) | A method of rolling into a ball flower nursery | |
| CN105684738A (en) | Sunlight greenhouse tomato culture method | |
| CN101695250A (en) | Quick breeding method for Phoebe chekiangensis seedling | |
| CN1711825A (en) | Water floatation seedling method of cotton | |
| CN105746224A (en) | Greenhouse seedling cultivating method for kalopanax septemlobus seeds | |
| CN110036907B (en) | Application of combination of chemical mutagenesis method and negative pressure method in polyploid breeding of Chinese fringetree | |
| CN102318546B (en) | Method for growing container seedlings of cork oak in winter | |
| CN113491223A (en) | Method for breeding thesium Chinese seeds | |
| CN107926715A (en) | A kind of eggplant or/and the engrafting and cultivating method of capsicum or/and tomato | |
| CN105453753B (en) | Method for treating fritillaria cirrhosa seeds and fritillaria cirrhosa cultivation method | |
| CN104642000B (en) | Manglietia lucida seed germinating and seedling method | |
| CN110226440B (en) | Method for growing seedlings of Nitraria tangutorum bobr in open field | |
| CN106888948A (en) | A kind of seed propagation method of first hat rattan | |
| CN110476764B (en) | Industrial paris polyphylla seedling raising method | |
| CN106665304A (en) | Jujube tree hybrid seed rapid seedling method based on soilless culture | |
| CN107736210B (en) | High-efficiency breeding method for beautiful millettia root seedlings | |
| CN106489477B (en) | A kind of Ecology Chinese podophyllum root method for culturing seedlings | |
| CN113767803B (en) | Rapid seedling raising method based on xanthoceras sorbifolia seed grading | |
| CN115152354A (en) | Method for efficiently breeding precious medicinal plant taxus chinensis | |
| CN113016474B (en) | Seedling raising method for radix zanthoxyli seedlings | |
| CN100499997C (en) | Ainsliaea fragrans champ breeding method | |
| GB2609995A (en) | Cultivation method of seedling from seed of wild ancient tea tree and method for preparing seedling tea | |
| CN210168584U (en) | Double-deck cultivation big-arch shelter that encircles | |
| CN101473792A (en) | Tissue culture of Ypsilandra thibetica and planting method | |
| CN110915539B (en) | Technology for raising seedlings and forestation of streblus streblumea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20210625 Assignee: Guangxi Weisen Biotechnology Group Co.,Ltd. Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS Contract record no.: X2023980045127 Denomination of invention: Method of Seedling Cultivation for Double Faced Needle Seedlings Granted publication date: 20230908 License type: Common License Record date: 20231101 |
|
| EE01 | Entry into force of recordation of patent licensing contract |