CN111902545B - 尿液样本保存及dna提取的组合物及方法 - Google Patents

尿液样本保存及dna提取的组合物及方法 Download PDF

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CN111902545B
CN111902545B CN202080000356.9A CN202080000356A CN111902545B CN 111902545 B CN111902545 B CN 111902545B CN 202080000356 A CN202080000356 A CN 202080000356A CN 111902545 B CN111902545 B CN 111902545B
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certain embodiments
urine sample
days
concentration
dna
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CN111902545A (zh
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吴扬
刘刚
吕宁
陈一友
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Hangzhou New Horizon Health Technology Co Ltd
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Hangzhou New Horizon Health Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/493—Physical analysis of biological material of liquid biological material urine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus

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  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
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  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
CN202080000356.9A 2019-01-03 2020-01-03 尿液样本保存及dna提取的组合物及方法 Active CN111902545B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2019070276 2019-01-03
CNPCT/CN2019/070276 2019-01-03
PCT/CN2020/070292 WO2020140975A1 (en) 2019-01-03 2020-01-03 Compositions and methods for urine sample storage and dna extraction

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CN111902545A CN111902545A (zh) 2020-11-06
CN111902545B true CN111902545B (zh) 2024-09-06

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US (1) US20220081705A1 (https=)
EP (1) EP3906319A4 (https=)
JP (1) JP2022516175A (https=)
KR (1) KR20210111250A (https=)
CN (1) CN111902545B (https=)
AU (1) AU2020205015A1 (https=)
BR (1) BR112021012224A2 (https=)
CA (1) CA3119928A1 (https=)
SG (1) SG11202106788UA (https=)
TW (2) TW202233846A (https=)
WO (1) WO2020140975A1 (https=)

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CN114574486B (zh) * 2020-12-01 2024-04-16 中国科学院大连化学物理研究所 作用于OPLAH的siRNA、DNA及构建物和应用
CN112592961A (zh) * 2021-01-07 2021-04-02 山东科硕生物技术有限公司 一种核酸样本保存液及其制备方法和应用
US20220315992A1 (en) * 2021-04-06 2022-10-06 ID Match, LLC Method for identification of urine drug testing sample
CN115521996A (zh) * 2021-10-25 2022-12-27 杭州诺辉健康科技有限公司 用于鼻咽癌诊断的试剂和方法
CN114935562B (zh) * 2022-04-11 2025-06-27 吉林大学 一种基于金纳米团簇超分子组装体的荧光探针及其在全氟辛磺酸检测中的应用
CN114959111B (zh) * 2022-05-07 2024-10-18 英科新创（苏州）生物科技有限公司 用于快速检测猫瘟病毒的试剂或试剂盒、其应用及检测方法
CN114875022B (zh) * 2022-05-31 2024-01-19 圣湘生物科技股份有限公司 尿液保存液、保存方法和尿液保存管
KR102690427B1 (ko) * 2022-06-29 2024-07-31 주식회사 어큐진 Dna, rna 동시추출 키트 및 이를 이용한 dna, rna 동시추출 방법
CN115363637A (zh) * 2022-08-23 2022-11-22 宁波海尔施基因科技股份有限公司 一种唾液样本采集卡及利用该采集卡采集唾液样本的方法
CN115901401B (zh) * 2023-01-09 2023-05-26 深圳市森盈生物科技有限公司 一种p16免疫细胞化学染色试剂盒及染色方法
US20250034547A1 (en) * 2023-07-25 2025-01-30 Promega Corporation Compositions and methods for the purification and concentration of nucleic acids from large-volume samples
CN116891849B (zh) * 2023-09-11 2023-11-17 成都斯马特科技有限公司 一种快速从鼻咽拭子中提取核酸的试剂盒及提取方法
CN119710095B (zh) * 2025-02-21 2025-07-01 浙江清华长三角研究院 检测hpv基因型的组合物、试剂盒及方法

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TWI766225B (zh) 2022-06-01
KR20210111250A (ko) 2021-09-10
EP3906319A1 (en) 2021-11-10
TW202233846A (zh) 2022-09-01
WO2020140975A1 (en) 2020-07-09
TW202039839A (zh) 2020-11-01
EP3906319A4 (en) 2022-11-16
SG11202106788UA (en) 2021-07-29
BR112021012224A2 (pt) 2021-09-28
AU2020205015A1 (en) 2021-06-03
JP2022516175A (ja) 2022-02-24
CN111902545A (zh) 2020-11-06
CA3119928A1 (en) 2020-07-09
US20220081705A1 (en) 2022-03-17

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