CN111656188A - 试剂、分析血小板的方法及血液细胞分析仪 - Google Patents
试剂、分析血小板的方法及血液细胞分析仪 Download PDFInfo
- Publication number
- CN111656188A CN111656188A CN201980008289.2A CN201980008289A CN111656188A CN 111656188 A CN111656188 A CN 111656188A CN 201980008289 A CN201980008289 A CN 201980008289A CN 111656188 A CN111656188 A CN 111656188A
- Authority
- CN
- China
- Prior art keywords
- reagent
- scattered light
- light intensity
- blood sample
- dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 177
- 238000000034 method Methods 0.000 title claims abstract description 93
- 210000000601 blood cell Anatomy 0.000 title claims abstract description 38
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 132
- 210000004369 blood Anatomy 0.000 claims abstract description 95
- 239000008280 blood Substances 0.000 claims abstract description 95
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 72
- 210000001995 reticulocyte Anatomy 0.000 claims abstract description 48
- 239000012528 membrane Substances 0.000 claims abstract description 34
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 31
- 230000002438 mitochondrial effect Effects 0.000 claims abstract description 28
- 239000000975 dye Substances 0.000 claims description 104
- -1 MitoLite Red Chemical compound 0.000 claims description 45
- 238000001514 detection method Methods 0.000 claims description 38
- 238000004458 analytical method Methods 0.000 claims description 31
- 230000003287 optical effect Effects 0.000 claims description 31
- 210000003743 erythrocyte Anatomy 0.000 claims description 30
- 238000002156 mixing Methods 0.000 claims description 25
- 229930182470 glycoside Natural products 0.000 claims description 23
- 150000007523 nucleic acids Chemical group 0.000 claims description 19
- 102000039446 nucleic acids Human genes 0.000 claims description 19
- 108020004707 nucleic acids Proteins 0.000 claims description 19
- 239000002245 particle Substances 0.000 claims description 19
- 150000007524 organic acids Chemical class 0.000 claims description 16
- 239000000980 acid dye Substances 0.000 claims description 15
- IKEOZQLIVHGQLJ-UHFFFAOYSA-M mitoTracker Red Chemical group [Cl-].C1=CC(CCl)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 IKEOZQLIVHGQLJ-UHFFFAOYSA-M 0.000 claims description 15
- 238000004883 computer application Methods 0.000 claims description 13
- MYFATKRONKHHQL-UHFFFAOYSA-N rhodamine 123 Chemical compound [Cl-].COC(=O)C1=CC=CC=C1C1=C2C=CC(=[NH2+])C=C2OC2=CC(N)=CC=C21 MYFATKRONKHHQL-UHFFFAOYSA-N 0.000 claims description 13
- 239000012103 Alexa Fluor 488 Substances 0.000 claims description 12
- 239000002736 nonionic surfactant Substances 0.000 claims description 11
- 239000012634 fragment Substances 0.000 claims description 10
- 150000003839 salts Chemical class 0.000 claims description 10
- 150000002772 monosaccharides Chemical group 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 6
- 150000004676 glycans Chemical class 0.000 claims description 6
- 229920001282 polysaccharide Polymers 0.000 claims description 6
- 239000005017 polysaccharide Substances 0.000 claims description 6
- RUVJFMSQTCEAAB-UHFFFAOYSA-M 2-[3-[5,6-dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]benzimidazol-2-ylidene]prop-1-enyl]-3-methyl-1,3-benzoxazol-3-ium;chloride Chemical compound [Cl-].O1C2=CC=CC=C2[N+](C)=C1C=CC=C(N(C1=CC(Cl)=C(Cl)C=C11)CC=2C=CC(CCl)=CC=2)N1CC1=CC=C(CCl)C=C1 RUVJFMSQTCEAAB-UHFFFAOYSA-M 0.000 claims description 5
- XXACTDWGHQXLGW-UHFFFAOYSA-M Janus Green B chloride Chemical compound [Cl-].C12=CC(N(CC)CC)=CC=C2N=C2C=CC(\N=N\C=3C=CC(=CC=3)N(C)C)=CC2=[N+]1C1=CC=CC=C1 XXACTDWGHQXLGW-UHFFFAOYSA-M 0.000 claims description 5
- 229910052783 alkali metal Inorganic materials 0.000 claims description 5
- 238000010586 diagram Methods 0.000 claims description 5
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 150000008130 triterpenoid saponins Chemical class 0.000 claims description 5
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 4
- 150000002338 glycosides Chemical group 0.000 claims description 4
- 235000005985 organic acids Nutrition 0.000 claims description 4
- 229930002600 steroidal saponin Natural products 0.000 claims description 4
- 125000000542 sulfonic acid group Chemical group 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 3
- 210000003714 granulocyte Anatomy 0.000 claims description 3
- 238000000149 argon plasma sintering Methods 0.000 claims description 2
- 230000008774 maternal effect Effects 0.000 claims 2
- 230000002949 hemolytic effect Effects 0.000 abstract description 20
- 206010018910 Haemolysis Diseases 0.000 abstract description 15
- 230000008588 hemolysis Effects 0.000 abstract description 15
- 210000004027 cell Anatomy 0.000 description 26
- 238000010186 staining Methods 0.000 description 20
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- 238000005259 measurement Methods 0.000 description 17
- 239000003795 chemical substances by application Substances 0.000 description 15
- 210000003979 eosinophil Anatomy 0.000 description 14
- 210000004698 lymphocyte Anatomy 0.000 description 14
- 210000001616 monocyte Anatomy 0.000 description 14
- 210000000440 neutrophil Anatomy 0.000 description 14
- 238000012360 testing method Methods 0.000 description 13
- 230000005284 excitation Effects 0.000 description 11
- 238000011534 incubation Methods 0.000 description 11
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 239000012535 impurity Substances 0.000 description 9
- JVXZRNYCRFIEGV-UHFFFAOYSA-M dilC18(3) dye Chemical compound [O-]Cl(=O)(=O)=O.CC1(C)C2=CC=CC=C2N(CCCCCCCCCCCCCCCCCC)C1=CC=CC1=[N+](CCCCCCCCCCCCCCCCCC)C2=CC=CC=C2C1(C)C JVXZRNYCRFIEGV-UHFFFAOYSA-M 0.000 description 8
- 239000007850 fluorescent dye Substances 0.000 description 8
- 210000000170 cell membrane Anatomy 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- 230000004069 differentiation Effects 0.000 description 7
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- 238000012757 fluorescence staining Methods 0.000 description 6
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 6
- 235000000346 sugar Nutrition 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 5
- JLIOTPLALDYAEH-UHFFFAOYSA-M diIC18(7) dye Chemical compound [I-].CC1(C)C2=CC=CC=C2N(CCCCCCCCCCCCCCCCCC)C1=CC=CC=CC=CC1=[N+](CCCCCCCCCCCCCCCCCC)C2=CC=CC=C2C1(C)C JLIOTPLALDYAEH-UHFFFAOYSA-M 0.000 description 5
- GFZPJHFJZGRWMQ-UHFFFAOYSA-M diOC18(3) dye Chemical compound [O-]Cl(=O)(=O)=O.O1C2=CC=CC=C2[N+](CCCCCCCCCCCCCCCCCC)=C1C=CC=C1N(CCCCCCCCCCCCCCCCCC)C2=CC=CC=C2O1 GFZPJHFJZGRWMQ-UHFFFAOYSA-M 0.000 description 5
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 125000000217 alkyl group Chemical group 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 238000000386 microscopy Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000007430 reference method Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 229940073499 decyl glucoside Drugs 0.000 description 3
- NLEBIOOXCVAHBD-QKMCSOCLSA-N dodecyl beta-D-maltoside Chemical compound O[C@@H]1[C@@H](O)[C@H](OCCCCCCCCCCCC)O[C@H](CO)[C@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 NLEBIOOXCVAHBD-QKMCSOCLSA-N 0.000 description 3
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 230000002101 lytic effect Effects 0.000 description 3
- 210000003470 mitochondria Anatomy 0.000 description 3
- 125000001421 myristyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 3
- ITMCEJHCFYSIIV-UHFFFAOYSA-M triflate Chemical compound [O-]S(=O)(=O)C(F)(F)F ITMCEJHCFYSIIV-UHFFFAOYSA-M 0.000 description 3
- 125000004169 (C1-C6) alkyl group Chemical group 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 206010027540 Microcytosis Diseases 0.000 description 2
- 239000008186 active pharmaceutical agent Substances 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 238000004820 blood count Methods 0.000 description 2
- 235000011090 malic acid Nutrition 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- HEGSGKPQLMEBJL-RKQHYHRCSA-N octyl beta-D-glucopyranoside Chemical compound CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HEGSGKPQLMEBJL-RKQHYHRCSA-N 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- CIYKWVNUXJDNNK-UHFFFAOYSA-N oxazine-750 Chemical compound N1=C2C3=CC=CC=C3C(NCC)=CC2=[O+]C2=C1C=C1CCCN3CCCC2=C13 CIYKWVNUXJDNNK-UHFFFAOYSA-N 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- HBTWOEFPWHHORE-UHFFFAOYSA-M (2e)-1-hexadecyl-2-[(e)-3-(1-hexadecyl-3,3-dimethylindol-1-ium-2-yl)prop-2-enylidene]-3,3-dimethylindole;perchlorate Chemical compound [O-]Cl(=O)(=O)=O.CC1(C)C2=CC=CC=C2N(CCCCCCCCCCCCCCCC)\C1=C\C=C\C1=[N+](CCCCCCCCCCCCCCCC)C2=CC=CC=C2C1(C)C HBTWOEFPWHHORE-UHFFFAOYSA-M 0.000 description 1
- UANMYOBKUNUUTR-UHFFFAOYSA-M (2z)-1,3,3-trimethyl-2-[(2e)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indole;iodide Chemical compound [I-].CC1(C)C2=CC=CC=C2N(C)C1=CC=CC=CC1=[N+](C)C2=CC=CC=C2C1(C)C UANMYOBKUNUUTR-UHFFFAOYSA-M 0.000 description 1
- ANJZLSIIEGUCQL-UHFFFAOYSA-M (2z)-3-heptyl-2-[(e)-3-(3-heptyl-1,3-benzoxazol-3-ium-2-yl)prop-2-enylidene]-1,3-benzoxazole;iodide Chemical compound [I-].O1C2=CC=CC=C2[N+](CCCCCCC)=C1/C=C/C=C1/N(CCCCCCC)C2=CC=CC=C2O1 ANJZLSIIEGUCQL-UHFFFAOYSA-M 0.000 description 1
- VRYALKFFQXWPIH-PBXRRBTRSA-N (3r,4s,5r)-3,4,5,6-tetrahydroxyhexanal Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)CC=O VRYALKFFQXWPIH-PBXRRBTRSA-N 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- CDLMLYASVIQVPH-UHFFFAOYSA-M 3-pentyl-2-[3-(3-pentyl-1,3-benzoxazol-3-ium-2-yl)prop-2-enylidene]-1,3-benzoxazole;iodide Chemical compound [I-].O1C2=CC=CC=C2[N+](CCCCC)=C1\C=C\C=C1/N(CCCCC)C2=CC=CC=C2O1 CDLMLYASVIQVPH-UHFFFAOYSA-M 0.000 description 1
- LWWWYHFXRJVZBL-UHFFFAOYSA-M 3-propyl-2-[3-(3-propyl-1,3-benzoxazol-3-ium-2-yl)prop-2-enylidene]-1,3-benzoxazole;iodide Chemical compound [I-].O1C2=CC=CC=C2[N+](CCC)=C1/C=C/C=C1/N(CCC)C2=CC=CC=C2O1 LWWWYHFXRJVZBL-UHFFFAOYSA-M 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 239000012099 Alexa Fluor family Substances 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- FIZZUEJIOKEFFZ-UHFFFAOYSA-M C3-oxacyanine Chemical compound [I-].O1C2=CC=CC=C2[N+](CC)=C1C=CC=C1N(CC)C2=CC=CC=C2O1 FIZZUEJIOKEFFZ-UHFFFAOYSA-M 0.000 description 1
- VZBILKJHDPEENF-UHFFFAOYSA-M C3-thiacarbocyanine Chemical compound [I-].S1C2=CC=CC=C2[N+](CC)=C1C=CC=C1N(CC)C2=CC=CC=C2S1 VZBILKJHDPEENF-UHFFFAOYSA-M 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- JDRSMPFHFNXQRB-CMTNHCDUSA-N Decyl beta-D-threo-hexopyranoside Chemical compound CCCCCCCCCCO[C@@H]1O[C@H](CO)C(O)[C@H](O)C1O JDRSMPFHFNXQRB-CMTNHCDUSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- SRBFZHDQGSBBOR-STGXQOJASA-N alpha-D-lyxopyranose Chemical compound O[C@@H]1CO[C@H](O)[C@@H](O)[C@H]1O SRBFZHDQGSBBOR-STGXQOJASA-N 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 description 1
- 229940092714 benzenesulfonic acid Drugs 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 125000002704 decyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- ZQSBJPAQPRVNHU-UHFFFAOYSA-M dilC18(5) dye Chemical compound [O-]Cl(=O)(=O)=O.CC1(C)C2=CC=CC=C2N(CCCCCCCCCCCCCCCCCC)C1=CC=CC=CC1=[N+](CCCCCCCCCCCCCCCCCC)C2=CC=CC=C2C1(C)C ZQSBJPAQPRVNHU-UHFFFAOYSA-M 0.000 description 1
- XVLXYDXJEKLXHN-UHFFFAOYSA-M dioc6 Chemical compound [I-].O1C2=CC=CC=C2[N+](CCCCCC)=C1C=CC=C1N(CCCCCC)C2=CC=CC=C2O1 XVLXYDXJEKLXHN-UHFFFAOYSA-M 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000003617 erythrocyte membrane Anatomy 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- PYIDGJJWBIBVIA-UYTYNIKBSA-N lauryl glucoside Chemical compound CCCCCCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PYIDGJJWBIBVIA-UYTYNIKBSA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- PSZYNBSKGUBXEH-UHFFFAOYSA-N naphthalene-1-sulfonic acid Chemical compound C1=CC=C2C(S(=O)(=O)O)=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-N 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- GHTWDWCFRFTBRB-UHFFFAOYSA-M oxazine-170 Chemical compound [O-]Cl(=O)(=O)=O.N1=C2C3=CC=CC=C3C(NCC)=CC2=[O+]C2=C1C=C(C)C(N(C)CC)=C2 GHTWDWCFRFTBRB-UHFFFAOYSA-M 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000005856 steroid saponins Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 150000003628 tricarboxylic acids Chemical class 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/011—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells with lysing, e.g. of erythrocytes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/018—Platelets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Ecology (AREA)
- Dispersion Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
一种试剂、使用该试剂在溶血条件下区分血小板的方法及血液细胞分析仪。该试剂包括:作为溶血剂的第一试剂;以及第二试剂,该第二试剂为膜特异性染料或线粒体特异性染料。使用该试剂、方法及分析仪可以在溶血条件实现对血小板的区分和网织红细胞报警,进一步的可以对白细胞进行分类计数,从而能够快速准确地在单一通道内对血样进行分析。
Description
PCT国内申请,说明书已公开。
Claims (33)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810402750 | 2018-04-28 | ||
CN201810402750X | 2018-04-28 | ||
PCT/CN2019/084648 WO2019206298A1 (zh) | 2018-04-28 | 2019-04-26 | 试剂、分析血小板的方法及血液细胞分析仪 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111656188A true CN111656188A (zh) | 2020-09-11 |
CN111656188B CN111656188B (zh) | 2021-10-12 |
Family
ID=68293483
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201980008289.2A Active CN111656188B (zh) | 2018-04-28 | 2019-04-26 | 试剂、分析血小板的方法及血液细胞分析仪 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20210041341A1 (zh) |
CN (1) | CN111656188B (zh) |
WO (1) | WO2019206298A1 (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113959912A (zh) * | 2020-07-20 | 2022-01-21 | 深圳迈瑞生物医疗电子股份有限公司 | 抗血小板聚集干扰的白细胞检测方法、试剂及其应用 |
WO2022115982A1 (zh) * | 2020-12-01 | 2022-06-09 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析方法、样本分析仪及计算机可读存储介质 |
WO2023028835A1 (zh) * | 2021-08-31 | 2023-03-09 | 深圳迈瑞动物医疗科技股份有限公司 | 一种样本分析装置和样本分析方法 |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018148942A1 (zh) * | 2017-02-17 | 2018-08-23 | 深圳迈瑞生物医疗电子股份有限公司 | 血液细胞分析方法及血液细胞分析仪 |
CN114252386A (zh) * | 2020-09-23 | 2022-03-29 | 深圳迈瑞生物医疗电子股份有限公司 | 样本检测方法和样本分析仪 |
CN117529646A (zh) * | 2021-12-31 | 2024-02-06 | 深圳迈瑞动物医疗科技股份有限公司 | 动物网织红细胞检测方法、样本分析仪 |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4336029A (en) * | 1980-08-15 | 1982-06-22 | Ortho Diagnostic Systems Inc. | Method and reagents for quantitative determination of reticulocytes and platelets in whole blood |
WO1996004544A1 (en) * | 1994-08-01 | 1996-02-15 | Abbott Laboratories | Method and apparatus for performing automated analysis |
WO2000058727A1 (en) * | 1999-03-31 | 2000-10-05 | Bayer Corporation | Single channel, single dilution detection method |
CN1323395A (zh) * | 1998-10-20 | 2001-11-21 | 库尔特国际公司 | 鉴别网状细胞的试剂组合物和方法 |
CN1981187A (zh) * | 2004-05-14 | 2007-06-13 | 霍尼韦尔国际公司 | 便携式样本分析仪盒 |
CN101173921A (zh) * | 2006-10-30 | 2008-05-07 | 希森美康株式会社 | 试剂、试剂盒及测定方法 |
CN101490547A (zh) * | 2006-07-17 | 2009-07-22 | 海莫库公司 | 血小板的计数 |
CN101750274A (zh) * | 2008-12-17 | 2010-06-23 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法 |
CN103323582A (zh) * | 2013-06-18 | 2013-09-25 | 南京普朗医疗设备有限公司 | 一种白细胞分类溶血剂及其试剂盒 |
CN103424540A (zh) * | 2012-05-18 | 2013-12-04 | 嘉善加斯戴克医疗器械有限公司 | 一种白细胞分类试剂盒及其分类方法 |
CN106525666A (zh) * | 2015-09-14 | 2017-03-22 | 希森美康株式会社 | 血液分析装置、血液分析方法及信息处理装置 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005024472A (ja) * | 2003-07-04 | 2005-01-27 | Sysmex Corp | 幼若血小板測定装置 |
US7390662B2 (en) * | 2005-11-09 | 2008-06-24 | Beckman Coulter, Inc. | Method and apparatus for performing platelet measurement |
CN101988082B (zh) * | 2009-07-31 | 2015-04-08 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 |
JP6001425B2 (ja) * | 2012-11-26 | 2016-10-05 | シスメックス株式会社 | 血球分析方法、血球分析装置およびプログラム |
CN104749144A (zh) * | 2013-12-31 | 2015-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | 血细胞检测试剂及血细胞处理方法和识别方法 |
JP2019500395A (ja) * | 2015-12-28 | 2019-01-10 | イースタン バージニア メディカル スクール | 卵母細胞のミトコンドリアマイクロインジェクション |
EP3258274B1 (en) * | 2016-06-17 | 2019-11-06 | Sysmex Corporation | Method of controlling a blood analyzer for measuring platelets |
-
2019
- 2019-04-26 WO PCT/CN2019/084648 patent/WO2019206298A1/zh active Application Filing
- 2019-04-26 CN CN201980008289.2A patent/CN111656188B/zh active Active
-
2020
- 2020-10-27 US US17/081,712 patent/US20210041341A1/en active Pending
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4336029A (en) * | 1980-08-15 | 1982-06-22 | Ortho Diagnostic Systems Inc. | Method and reagents for quantitative determination of reticulocytes and platelets in whole blood |
WO1996004544A1 (en) * | 1994-08-01 | 1996-02-15 | Abbott Laboratories | Method and apparatus for performing automated analysis |
CN1323395A (zh) * | 1998-10-20 | 2001-11-21 | 库尔特国际公司 | 鉴别网状细胞的试剂组合物和方法 |
WO2000058727A1 (en) * | 1999-03-31 | 2000-10-05 | Bayer Corporation | Single channel, single dilution detection method |
CN1981187A (zh) * | 2004-05-14 | 2007-06-13 | 霍尼韦尔国际公司 | 便携式样本分析仪盒 |
CN101490547A (zh) * | 2006-07-17 | 2009-07-22 | 海莫库公司 | 血小板的计数 |
CN101173921A (zh) * | 2006-10-30 | 2008-05-07 | 希森美康株式会社 | 试剂、试剂盒及测定方法 |
CN101750274A (zh) * | 2008-12-17 | 2010-06-23 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法 |
CN103424540A (zh) * | 2012-05-18 | 2013-12-04 | 嘉善加斯戴克医疗器械有限公司 | 一种白细胞分类试剂盒及其分类方法 |
CN103323582A (zh) * | 2013-06-18 | 2013-09-25 | 南京普朗医疗设备有限公司 | 一种白细胞分类溶血剂及其试剂盒 |
CN106525666A (zh) * | 2015-09-14 | 2017-03-22 | 希森美康株式会社 | 血液分析装置、血液分析方法及信息处理装置 |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113959912A (zh) * | 2020-07-20 | 2022-01-21 | 深圳迈瑞生物医疗电子股份有限公司 | 抗血小板聚集干扰的白细胞检测方法、试剂及其应用 |
WO2022115982A1 (zh) * | 2020-12-01 | 2022-06-09 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析方法、样本分析仪及计算机可读存储介质 |
WO2023028835A1 (zh) * | 2021-08-31 | 2023-03-09 | 深圳迈瑞动物医疗科技股份有限公司 | 一种样本分析装置和样本分析方法 |
Also Published As
Publication number | Publication date |
---|---|
US20210041341A1 (en) | 2021-02-11 |
WO2019206298A1 (zh) | 2019-10-31 |
CN111656188B (zh) | 2021-10-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111656188B (zh) | 试剂、分析血小板的方法及血液细胞分析仪 | |
EP0483116B1 (en) | Method of classifying leukocytes by flow cytometry and reagents used in the method | |
EP2267443B1 (en) | Determination of white blood cell differential and reticulocyte counts | |
US7625712B2 (en) | Method for a fully automated monoclonal antibody-based extended differential | |
CN111602052B (zh) | 一种血液检测方法及血液分析系统 | |
WO2012147451A1 (ja) | 血液分析装置、血液分析方法、及びコンピュータプログラム | |
WO2016106688A1 (zh) | 一种有核红细胞报警方法、装置及流式细胞分析仪 | |
US9797824B2 (en) | Method for hematology analysis | |
WO2008002745A2 (en) | Method for a fully automated monoclonal antibody-based extended differential | |
JPH10282094A (ja) | 全血中の網状赤血球、赤血球及び血小板を迅速に同定及び特徴付けるための完全に自動化された方法及びそれらのための試薬組成物 | |
US5179026A (en) | Method of classifying leukocytes by flow cytometry and reagents used in the method | |
US20210033592A1 (en) | Blood analyzer and analysis method | |
JP2009080122A (ja) | 赤芽球の分類計数方法 | |
US20230296591A1 (en) | Sample analysis method, sample analyzer, and computer-readable storage medium | |
JPH07113632B2 (ja) | 白血球分析方法 | |
Ronot et al. | Assessment of cell viability in mammalian cell lines | |
EP0259833A2 (en) | Reagent and method for classifying leukocytes by flow cytometry | |
CN115201156A (zh) | 分析血液样本中红细胞的方法及血液分析系统 | |
CN115201161A (zh) | 试剂、血液细胞分析方法及分析仪 | |
CN118258993A (zh) | 用于血液分析的试剂、血液分析方法及血液分析系统 | |
CN115201153A (zh) | 血液检测方法和血液分析系统 | |
CN114270167A (zh) | 血液检测方法及血液分析系统 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |